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Team:NYMU-Taipei/modeling/m1
From 2014.igem.org
Purpose
Introduction: Growth Curve
A growth curve for a population of bacteria illustrates some of the dynamics that affect the population size over time.
Four distinct phases are recognized:
The lag phase:
The curve remains at a plateau. During this time, bacteria adapt to their new environment, store nutrients and prepare for binary fission.
The logarithmic phase:
This phase is also called the exponential growth phase: the population of bacteria enters an active stage of growth, the mass of each cell increases rapidly, and the number of bacteria doubles.
The stationary phase:
At this stage reproductive and death rates equalize, the population enters another plateau.
Models and mathematic equations
Three well known growth models (Logistic, Gompertz, and Richartz)are used in this work. Characteristic model parameters (such as lag phase (λ), maximal growth rate (µ-max slope), stationary phase (A-max growth value)) are derived from our experimental data. Bootstrap and cross-validation techniques are used for estimating confidence intervals of
all derived parameters.
The aim is to integrate the experimental data into different growth models and to compare the models using statistical methods (AIC and maximum likelihood were used). We believe, and many scientists do, that model selection is the most important part in model-experiment based research-The right data with the right model.
Logistic Model: $$\begin{align} y(t)&=\frac{A}{1+\exp\left(\frac{4\mu}{A}(\lambda-t)+2\right)} \end{align}$$ Gompertz Model: $$\begin{align} y(t)&=A.\exp\left[-\exp\left(\frac{\mu.\exp(1)}{A}(\lambda-t)+1\right)\right] \end{align}$$ Richartz Model: \begin{align} y(t)&=A.\left[1+\nu\exp\left(1+\nu+\frac{\mu}{A}(1+\nu)^{1+1/\nu}(\lambda-t)\right)\right]^{-1/\nu} \end{align} $\nu$ is a shape parameter (in the richartz model only)
pH model
Existed model is \begin{align} \frac{dpH}{dP}&=k(pH-pH_{min}) %pH&=pH_{min}+\left(pH_{0}-pH_{min}\right)^{-k(P-P_{0})} \end{align} However, this model is not appropriate for our experimental data; we used linear regression model instead. \begin{align} \label{eq:reg} pH=\alpha + \beta OD +\epsilon \end{align} where $\epsilon$ is a random error or noice (which helps to capture a measurement error and other unknown factors). And it is assumed to be Gaussian (normal distribution function), $\epsilon = N(\theta,\sigma^{2})$, with mean $\theta$ and constant variance $\sigma^{2}$. $\alpha$ and $\beta$ are model parameters.
Result and model validation
Our experimental data are implemented in the three proposed models. AIC is used to measure the performance of the models and the result shows that Logistic and Richartz models are approximately the same, but slightly different from Gompertz. However, using 95% confidence interval all of them are appropriate to fit the given data.
The validation test (plot of the residuals), as Figure 2, shows that the simulation result obtained from bootstrap samples is suitable to estimate the model parameters.
Using our experimental data as initials samples, we applied Bootsrap statistical to sample empirical data. The simulated result is presented as Figure (1). And, the estimated model parameters (mu(µ), lamda(λ), A) are summarized as Table 1.
$$\textbf{Table 1: Estimated Model Parameter}$$
Reference
