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Team:NYMU-Taipei/notebook/labnotes
From 2014.igem.org
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<div id='labnote'> | <div id='labnote'> | ||
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<div class='note-content notenow' id='nt1-cont'> | <div class='note-content notenow' id='nt1-cont'> | ||
<h1>introduction :P introduction :P introduction :P introduction :P </h1> | <h1>introduction :P introduction :P introduction :P introduction :P </h1> | ||
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<p>under construction</p> | <p>under construction</p> | ||
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<div class='note-content' id='nt2-cont' style='display:none;'> | <div class='note-content' id='nt2-cont' style='display:none;'> | ||
<h1>lab note of circuit in the control part</h1> | <h1>lab note of circuit in the control part</h1> | ||
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<p>under construction</p> | <p>under construction</p> | ||
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<div class='note-content' id='nt3-cont' style='display:none;'> | <div class='note-content' id='nt3-cont' style='display:none;'> | ||
<h1>lab note of circuit in the communication part</h1> | <h1>lab note of circuit in the communication part</h1> | ||
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<p>under construction</p> | <p>under construction</p> | ||
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<div class='note-content' id='nt4-cont' style='display:none;'> | <div class='note-content' id='nt4-cont' style='display:none;'> | ||
<h1>lab note of circuit in the cohesion part</h1> | <h1>lab note of circuit in the cohesion part</h1> | ||
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<p>under construction</p> | <p>under construction</p> | ||
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<div class='note-content' id='nt5-cont' style='display:none;'> | <div class='note-content' id='nt5-cont' style='display:none;'> | ||
<h1>lab note of circuit in the completion part</h1> | <h1>lab note of circuit in the completion part</h1> | ||
| - | < | + | <h2>Parts from iGEM took kit</h2> |
| - | < | + | <h3>Out line</h3> |
| - | <p>part | + | <p>For each part to be used in testing circuit:<br>BBa_J23100 , BBa_E1010, BBa_B0015 |
| - | <p> | + | </p> |
| - | < | + | <img src=''><p>circuit 圖</p> |
| - | < | + | <p>After transforming these plasmids into our competent cell, DH5a, we would take 3 steps to examine if we got the right product:</p> |
| - | < | + | <ol> |
| - | < | + | <li>Colony PCR and electrophoresis: Directly picking up colonies on designated antibiotic plate and do colony PCR with iGEM provided primers (VF2&VR), and then do electrophoresis to check the length of plasmid to confirm if the kit had been correctly transformed into E. coli.</li> |
| - | + | <li>Plasmid PCR and electrophoresis: Colony PCR may not end up copying the right place, for there is extra DNA in the cell that is not the one of our interest. Therefore, we also check the length of the plasmid we which we transformed.</li> | |
| + | <li>Digestion and electrophoresis: After checking the length, we use EcoR1/Spe1 and Xba1/Pst1, and electrophoresis, to see if our constructed plasmid can be successfully digested.</li> | ||
| + | </ol> | ||
<h2>experiment1 date: 0707~0712</h2> | <h2>experiment1 date: 0707~0712</h2> | ||
<p>under construction</p> | <p>under construction</p> | ||
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<p>under construction</p> | <p>under construction</p> | ||
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<div class='note-content' id='nt6-cont' style='display:none;'> | <div class='note-content' id='nt6-cont' style='display:none;'> | ||
<h1>lab note of function test in phage</h1> | <h1>lab note of function test in phage</h1> | ||
Revision as of 17:45, 28 September 2014
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introduction
control
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cohesion
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function test
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introduction of labnote
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