Team:SDU-Denmark/Tour33

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Latest revision as of 21:42, 17 October 2014

Edible coli

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Edible coli

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SOPs

We used a lot of different Standard Operating Procedures (SOPs) during our project written by last years SDU iGEM team. It is very important that every person in the lab uses the same SOP to make sure that the experiments does not depend on the person executing them, but only on the methods used. Any changes made to the SOPs were agreed upon by the entire team, to make sure that everyone knew when and why a Standard Operating Procedure was changed. Below you can explore all our SOPs:

iGEM2013 SOP0001 v01 - ON culture of E. coli
iGEM2013 SOP0002 v01 - Table autoclave
iGEM2013 SOP0003 v01 - Bacterial freezing stock
iGEM2013 SOP0004 v01 - Primer Design
iGEM2013 SOP0005 v01 - USER primer design
iGEM2013 SOP0006 v01 - PCR protocol for USER cloning
iGEM2013 SOP0007 v01 - excision and ligation of PCR product in USER cloning
iGEM2013 SOP0008 v01 - preparing primers
iGEM2013 SOP0009 v01 - TSB transformation
iGEM2013 SOP0010 v01 - Phusion PCR
iGEM2013 SOP0012 v01 - Restriction Digest
iGEM2013 SOP0013 v01 - Nano-drop
iGEM2013 SOP0014 v01 - Gel purification
iGEM2013 SOP0016 v01 - LB and LA media
iGEM2013 SOP0018 v01 - Agar plates with Cm
iGEM2013 SOP0019 v01 - Plasmid Miniprep
iGEM2013 SOP0020 v01 - Agarose gel DNA
iGEM2013 SOP0021 v01 - MyTaq Colony PCR
iGEM2013 SOP0022 v01 - Speedy Vac
iGEM2013 SOP0024 v01 - Growth experiment
iGEM2014 SOP0011 v01 - WesternBlotting
iGEM2014 SOP0015 v02 - Ligation
iGEM2014 SOP0017 v01 - Fast digest

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-<h3> Introduction to modelling </h3>
+<h3>SOPs</h3>
 <p>
+<span class="intro">We used a lot</span>
+ of different Standard Operating Procedures (SOPs) during our project written by last years SDU iGEM team. It is very important that every person in the lab uses the same SOP to make sure that the experiments does not depend on the person executing them, but only on the methods used. Any changes made to the SOPs were agreed upon by the entire team, to make sure that everyone knew when and why a Standard Operating Procedure was changed.
+Below you can explore all our SOPs:
-Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nullam eget sapien eget magna tincidunt laoreet. Nunc nec euismod justo. Nulla facilisi. Proin dignissim, est nec tempus auctor, lorem ex elementum leo, non molestie odio risus ut velit. Nulla dapibus ex at enim mollis pellentesque ut vitae purus. Suspendisse potenti. Suspendisse a mattis neque. Nunc purus leo, gravida eu congue nec, vestibulum nec erat. Pellentesque venenatis nisl urna, elementum commodo eros auctor eu. Cras sit amet iaculis ex. Phasellus sed est dictum nisl semper varius eu in eros. Proin varius imperdiet ornare. Sed placerat lacus enim, vel efficitur lacus viverra a. Nullam facilisis metus vel odio dignissim fermentum. Sed dapibus turpis urna, vel euismod augue varius id. Curabitur et nibh faucibus metus blandit pellentesque eget ut sem.
+<br>
+<br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/ef/SDU2013_SOP0001_v01.pdf" target="_blank">iGEM2013 SOP0001 v01 - ON culture of <span class="specialWord">E. coli</span></a><br>
+<a class="pdfFile"href="https://static.igem.org/mediawiki/2013/5/5b/SDU2013_SOP0002_v01.pdf" target="_blank">iGEM2013 SOP0002 v01 - Table autoclave</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/88/SDU2013_SOP0003_v01.pdf" target="_blank">iGEM2013 SOP0003 v01 - Bacterial freezing stock</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/5/59/SDU2013_SOP0004_v01.pdf" target="_blank">iGEM2013 SOP0004 v01 - Primer Design</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/f/f2/SDU2013_SOP0005_v01.pdf" target="_blank">iGEM2013 SOP0005 v01 - USER primer design</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/73/SDU2013_SOP0006_v01.pdf" target="_blank">iGEM2013 SOP0006 v01 - PCR protocol for USER cloning</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/a/a1/SDU2013_SOP0007_v01.pdf" target="_blank">iGEM2013 SOP0007 v01  - excision and ligation of PCR product in USER cloning</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/6/6e/SDU2013_SOP0008_v01.pdf" target="_blank">iGEM2013 SOP0008 v01 - preparing primers</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/71/SDU2013_SOP0009_v01.pdf" target="_blank">iGEM2013 SOP0009 v01 - TSB transformation</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/9/93/SDU2013_SOP0010_v01.pdf" target="_blank">iGEM2013 SOP0010 v01 - Phusion PCR</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/74/SDU2013_SOP0012_v01.pdf" target="_blank">iGEM2013 SOP0012 v01  - Restriction Digest</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/c/cd/SDU2013_SOP0013_v01.pdf" target="_blank">iGEM2013 SOP0013 v01 - Nano-drop</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_SOP0014_v01.pdf" target="_blank">iGEM2013 SOP0014 v01 - Gel purification</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/2/2c/SDU2013_SOP0016_v01.pdf" target="_blank">iGEM2013 SOP0016 v01 - LB and LA media</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/ed/SDU2013_SOP0018_v01.pdf" target="_blank">iGEM2013 SOP0018 v01 - Agar plates with Cm</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/85/SDU2013_SOP0019_v01.pdf" target="_blank">iGEM2013 SOP0019 v01 - Plasmid Miniprep</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/b/b2/SDU2013_SOP0020_v01.pdf" target="_blank">iGEM2013 SOP0020 v01 - Agarose gel DNA</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/1/15/SDU2013_SOP0021_v01.pdf" target="_blank">iGEM2013 SOP0021 v01 - MyTaq Colony PCR</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/5/56/SDU2013_SOP0022_v01.pdf" target="_blank">iGEM2013 SOP0022 v01 - Speedy Vac</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/4/4e/SDU2013_SOP0024_v01.pdf" target="_blank">iGEM2013 SOP0024 v01 - Growth experiment</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2014/4/44/IGEM2014_SOP0011_v01_WesternBlotting.pdf" target="_blank">iGEM2014 SOP0011 v01 - WesternBlotting</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2014/e/e6/IGEM2014_SOP0015_v02_Ligation.pdf" target="_blank">iGEM2014 SOP0015 v02 - Ligation</a><br>
+<a class="pdfFile" href="https://static.igem.org/mediawiki/2014/c/c4/IGEM2014_SOP0017_v01_Fast_digest.pdf" target="_blank">iGEM2014 SOP0017 v01 - Fast digest</a><br>
+<br><br>
-Suspendisse tempus, augue vitae egestas elementum, erat felis cursus diam, bibendum pellentesque augue mauris eget ante. Pellentesque condimentum felis at lacinia ornare. Suspendisse ut mauris ligula. Nulla in feugiat lacus, at finibus erat. Fusce consectetur justo ac est elementum, in vulputate tellus pellentesque. Nunc a enim nunc. Ut malesuada massa nec hendrerit varius. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus.
 </p>
-<center> <a href="https://2014.igem.org/Team:SDU-Denmark/Tour34">Enzyme database</a> </center>
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+</html>
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