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Team:SDU-Denmark/Tour45
From 2014.igem.org
Submitted parts
Below is a list of the parts we submitted to the parts registry. To see sequencing, characterization etc. click the part names to go to the parts registry page.
Basic parts
BBa_K1475001
OneProt: The coding region of our self-designed nutritional protein. OneProt contains the recommended ratio of all the indispensible amino acids to a human diet. The protein is also compliant with the recommended ratio of indispensible to dispensable amino acids.
BBa_K1475002
fat-2: This part contains only the coding region of the fat-2 desaturase from Caenorhabditis elegans, the natural gene consists of three exons.
BBa_K1475003
TetR(N): the frequently used tet repressor from Escherichia coli (Part:BBa_C0040) modified by removing the LVA rapid degradation tag.
OneProt: The coding region of our self-designed nutritional protein. OneProt contains the recommended ratio of all the indispensible amino acids to a human diet. The protein is also compliant with the recommended ratio of indispensible to dispensable amino acids.
BBa_K1475002
fat-2: This part contains only the coding region of the fat-2 desaturase from Caenorhabditis elegans, the natural gene consists of three exons.
BBa_K1475003
TetR(N): the frequently used tet repressor from Escherichia coli (Part:BBa_C0040) modified by removing the LVA rapid degradation tag.
Regulatory devices
BBa_K1475004
Pcons-TetR(N)-term: the tet repressor from Escherichia coli is expressed form a constitutively active promoter. A terminator prevents transcription of genes downstream of the repressor.
BBa_K1475008
Pcon-LacI-term: Lac repressor protein derived from Escherichia coli without LVA degradation tag, controlled by a constitutively active promoter.
Pcons-TetR(N)-term: the tet repressor from Escherichia coli is expressed form a constitutively active promoter. A terminator prevents transcription of genes downstream of the repressor.
BBa_K1475008
Pcon-LacI-term: Lac repressor protein derived from Escherichia coli without LVA degradation tag, controlled by a constitutively active promoter.
Constitutively active production devices
BBa_K1475000
p(tetR)-OneProt-term: OneProt is a self-designed protein designed to meet the recommended ratio of indispensible amino acids to a human diet according to the world health organization (WHO). It also contains the recommended ratio of indispensible to dispensible amino acids.
p(tetR)-OneProt-term: OneProt is a self-designed protein designed to meet the recommended ratio of indispensible amino acids to a human diet according to the world health organization (WHO). It also contains the recommended ratio of indispensible to dispensible amino acids.
Regulable production devices
BBa_K1475005
Pcon-TetR(N)-term-p(TetR)-GFP-term: GFP controlled by a TetR, pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.
BBa_K1475006
Pcon-TetR(LVA)-term-p(TetR)-GFP-term: GFP controlled by a TetR+LVA pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.
BBa_K1475007
Pcon-LacI-term-p(LacI)-GFP-term: GFP controlled by the lac repressor and lac promoter system.
BBa_K1475012
Pcon-LacI-p(LacI)-GFP-term: GFP controlled by a LacI, p(LacI) system in which the LacI and p(Lac) sequence overlaps.
Pcon-TetR(N)-term-p(TetR)-GFP-term: GFP controlled by a TetR, pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.
BBa_K1475006
Pcon-TetR(LVA)-term-p(TetR)-GFP-term: GFP controlled by a TetR+LVA pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.
BBa_K1475007
Pcon-LacI-term-p(LacI)-GFP-term: GFP controlled by the lac repressor and lac promoter system.
BBa_K1475012
Pcon-LacI-p(LacI)-GFP-term: GFP controlled by a LacI, p(LacI) system in which the LacI and p(Lac) sequence overlaps.
