Team:SDU-Denmark/Tour30

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Revision as of 17:00, 17 October 2014 by Ulrikasimone (Talk | contribs)

Process

From idea to bricks

On the 20th of March, the team was assembled, and we had our first meeting on the 25th, where some of us met each other for the first time. A lot of expectations had to be matched, and we spent the next few weeks getting to know each other through team building.

In the beginning, the biggest process was to come up with an idea. Several brainstorming methods were used, but the list of ideas suddenly became endless, and an abatement process had begun! At our first introduction to the wet lab work, at the BioBrick workshop at DTU on the 2nd to 4th of May, we had cut the list down to just four ideas, which was discussed with the other two Danish teams attending the workshop; the University of Copenhagen and the Technical University of Denmark. A few days after the workshop, we finally decided on the project: Edible coli!

The next few weeks, we spent researching about how to design our system and the possibilities of our idea. The idea slowly evolved, and we decided on which bricks to use from the iGEM Registry of Standard Biological Parts and which bricks to make ourselves.

Mid July, we finally began working in the wet lab, starting out with crash course, organized by our instructor. Since then, we have been working day and night, both in- and outside the laboratory. We have all learned a lot during the summer, and had some diversity by all being, more or less, part of both the wet and dry lab process. Throughout the project, we have all helped each other, but the main responsibilities have been divided as follows:

Wet lab

  • Plasmid constructions: Martin, Camilla, Anne, Ulrika, Signe, Victoria, Jens Jakob, Daniel and Sarah (the whole team)
  • USER cloning: Daniel
  • FACS:Signe
  • Growth experiments: Martin, Victoria and Signe
  • Worm experiment: Martin and Jens Jakob
  • Western blots: Victoria
  • Dry lab

  • Modelling: Sarah and Jens Jakob
  • Primer design: Daniel and Ulrika
  • Protein design: Camilla
  • Sequence analysis: Jens Jakob and Daniel
  • Policy and practices: Anne, Signe and Victoria
  • Video adventure: Daniel and Ulrika
  • Wiki design: Sarah