Team:IvyTech SouthBend IN

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<h2>Introduction</h2>
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<h1 >WELCOME TO iGEM 2014! </h1>
 
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<p>Your team has been approved and you are ready to start the iGEM season!
 
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
 
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:IvyTech_SouthBend_IN&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
 
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<a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN"style="color:#000000">Home </a> </td>
 
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<a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/Team"style="color:#000000"> Team </a> </td>
 
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<a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/Project"style="color:#000000"> Project</a></td>
 
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<a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/Modeling"style="color:#000000"> Modeling</a></td>
 
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<h4>The rapid detection of coliphage in water</h4>
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<a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN/Attributions"style="color:#000000"> Attributions </a></td>
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<span class="intro">The need for rapid detection</span> Of coliphage is something that has been present for years. Coliphage is fecal-borne viruses. These can be present in virtually any body of water in any country in the world. The present method of coliphage detection takes at least 24 hours once the sample arrives at the lab. It requires a lab setting with delicate equipment and highly trained personnel. We are striving to create a handheld device that takes 1-3 hours and can be performed by anyone.
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<span class="intro">We plan</span> to use freeze-dried E. coli cells in a hand held device. The [potentially] contaminated water will be introduced in controlled volumes and lyse (burst) the cells. The amount of virus present will be indicated with a colorimetric reading.
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<span class="intro">We have constructed</span> a plasmid which we have transformed into a strain of <span class="specialWord">Escherichia coli</span>. This plasmid consists of a promoter, a mutant lacZ operon, and a terminator. The enzyme fragment produced by the lacZ operon is released upon lysis and seeks out its complement in vitro (outside of the cell.)The enzymatic activity of this complementation reacts with our indicator, chlorophenol red (CPRG) to produce a color change that is directly proportional to the amount of viruses present.
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<a class="popupImg alignLeft" style="width:340px" target="_blank" href="https://static.igem.org/mediawiki/2013/f/fd/SDU2013_Introduction_2.png" title="The chassis of our production system will be the bacteria <i>E. coli</i>.">
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<i>E. coli</i> is used as a catalyst for the detection of the T4/T7 coliphage virus.
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<span class="intro">This is</span>
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something that can be used both in industrialized countries and developing nations with equal value and opportunity for everyone
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<span class="intro">The team working on this project</span> consists of four undergraduate students pursuing degrees of both biotechnology and nanotechnology. Furthermore, our team was guided 2 supervisors from the Ivy Tech Community College staff; Professor Twaddle and Professor Arisio
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<li><a href="https://2014.igem.org/Team:IvyTech_SouthBend_IN">Home</a> </li>
 
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=IvyTech_SouthBend_IN">Official Team Profile</a> </li>
 
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<span class="intro">We would like to profusely thank</span> the SDU-Denmark team who provided the template for our wiki page. We could not have done it without them
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<p>There are a few wiki requirements teams must follow:</p>
 
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
 
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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Revision as of 15:44, 12 August 2014

Edible coli
iGEM logo
Introduction
Edible coli
Process
Results
Policy and practices
Future

Introduction

The rapid detection of coliphage in water

The need for rapid detection Of coliphage is something that has been present for years. Coliphage is fecal-borne viruses. These can be present in virtually any body of water in any country in the world. The present method of coliphage detection takes at least 24 hours once the sample arrives at the lab. It requires a lab setting with delicate equipment and highly trained personnel. We are striving to create a handheld device that takes 1-3 hours and can be performed by anyone.

We plan to use freeze-dried E. coli cells in a hand held device. The [potentially] contaminated water will be introduced in controlled volumes and lyse (burst) the cells. The amount of virus present will be indicated with a colorimetric reading.

We have constructed a plasmid which we have transformed into a strain of Escherichia coli. This plasmid consists of a promoter, a mutant lacZ operon, and a terminator. The enzyme fragment produced by the lacZ operon is released upon lysis and seeks out its complement in vitro (outside of the cell.)The enzymatic activity of this complementation reacts with our indicator, chlorophenol red (CPRG) to produce a color change that is directly proportional to the amount of viruses present.

E. coli is used as a catalyst for the detection of the T4/T7 coliphage virus.

This is something that can be used both in industrialized countries and developing nations with equal value and opportunity for everyone

The team working on this project consists of four undergraduate students pursuing degrees of both biotechnology and nanotechnology. Furthermore, our team was guided 2 supervisors from the Ivy Tech Community College staff; Professor Twaddle and Professor Arisio

We would like to profusely thank the SDU-Denmark team who provided the template for our wiki page. We could not have done it without them

Next chapter

Next chapter

Dig deeper

End of tour

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