Team:SDU-Denmark/Tour14

Attributions

"If you want to become a great chef, you have to work with great chefs" - Gordon Ramsay

Sponsors

We would like to thank our sponsor The University of Southern Denmark, for funding our iGEM project. Especially we would like to thank dean Henrik Pedersen and the Faculty of Science at University of Southern Denmark - we are truly grateful for having this amazing possibility.

Laboratory support

We would like to thank Associate Professor, Ph. D. Jakob Møller Jensen and the Microbiology group and also the rest of the Department of Biochemistry and Molecular Biology for letting us use their lab, providing laboratory equipment and helping us to do our work.

• Our instructors Academic Assistant Tina Kronborg, post doc Ann Zahle Andersen, stud.cand. Patrick Rosendahl Andreassen, stud.cand. Andreas Kjær and stud.cand. Thøger Jensen Krogh have helped us the entire summer, when crying for help. We are really grateful for all your help, in every situation.
• Ph.D. fellow Maria Storm Mollerup helped us with general questions in the lab.
• Academic assistant Eva Maria Sternkopf Lillebæk helped with western blot and provided us with a Bacillus subtilis strain.
• Ph.D. fellow Sabrina Brøner helped us getting doxycyclin.
• Post doc Anders Boysen helped with western blot.
• Medical Laboratory Technician Simon Rose introduced us to lab safety and behavior and helped us with our safety form.
• From the University of Copenhagen professor, Ph.D., head of Section for Molecular Plant Biology, vice head of Copenhagen Plant science Centre Poul Erik Jensen who sent us a Synechocystis sp. PCC6803 strain.
• Stud.Bsc.Sc. Kristian Davidsen from DTU provided us with USER polymerase.
• Professor, Ph.D. Nils Joakim Færgeman helped us design a GC experiment and the toxicity essay on Caenorhabditis elegans.
• Ph. D. fellow Eva Bang Harvald provided us with Caenorhabditis elegans and information about what to do.
• Professor Dr. rer. nat. habil. Olaf-Georg Issinger helped out with western blot gels.
• Ph.D. fellow from KU (University of Copenhagen) Nana Cecilie Halmsted Kongsholm helped with ethics.
• Postdoctoral fellow at the Medical Research Council Julius Fredens provided us with a plasmid containing FAT-2 originating from Caenorhabditis elegans.
• The SDU iGEM team 2013 for great inspiration for the making of our wiki.

Events support

• Student house Odense arranged an event where we could talk about iGEM and synthetic biology and hold a quiz night.
• Syddanske studerende let us promote our own project and iGEM by having us at the study trade fair.
• IMCC (International Medical Cooperation Committee) let us promote our own project and iGEM by having us at the study trade fair and at sundhedsmekka.
• The former SDU iGEM members who met with us and heard about our project and what thoughts we were having.

General support

• DTU (Technical University of Denmark) had arranged a crash course in the lab for Danish iGEM teams and introduced us to primers, USER cloning and the design of the team wiki.
• KU (University of Copenhagen) arranged an ethics workshop for Danish iGEM teams.
• YSB (young synthetic biologist) arranged and held the UK iGEM meet-up and allowed our team to join the meet up and told us more about synthetic biology and had arranged different workshops.
• Everyone all over the world, who has answered our questionnaire about GMO and what they think about it being a food resource.
• Jane Fornitz from the company Ibsing & Fornitz ApS introduced us to the different types of personalities and how to work together with different personalities - this has been a great help for the teamwork.
• Doctor Yaa Difie-Osei from the National Biosafety Committee, Ghana and Professor George Armah from the Noguchi memorial institute for medical research helped us with our human practices regarding outreach and ethical considerations.

Technical support

• Post doc Ann Zahle Andersen helped us with programs used for modelling.
• Stud.cand. Patrick Rosendahl Andreassen, stud.cand. Andreas Kjær and stud.cand. Thøger Jensen Krogh have helped us with sequencing results.
• Stud.cand. Thøger Jensen Krogh helped us designing our team wiki.

Modelling

• Stud.cand. Nicky Cordua Mattsson helped us with the modelling of our system.
• Post doc Ann Zahle Andersen helped us all the way with our model.

Litterature support

• MicroBEnet: Microbial Myths: Common misconceptions about microbes (w/ some extra focus on those in the built environment), 2011. (Link)
• Marris, C.: Public views on GMOs: deconstructing the myths. EMBO reports, 2001. Vol. 2 p. 545-548. (Link)
• Gaskell, G., Stares, S., Allansdottir, A., Allum, N., Corchero, C., Fischler, C., Hampel, J., Jackson, J., Kronberger, N., Mejlgaard, N., Revuelta, G., Schreiner, C., Torgersen, H., and Wagner, W.: Europeans and Biotechnology in 2005: Patterns and Trends. Final report on Eurobarometer 64.3, 2006. P. 57. (Link)
• Hancock, R.D.: Recent Patents on Vitamin C: Opportunities for Crop Improvement and Single-Step Biological Manufacture. Recent Patents on Food, Nutrition & Agriculture, 2009. Vol. 1, p. 39-49. (Link)
• Sauer, M., Porro, D, Mattanovich, D., and Branduardi, P.: Microbial production of organic acids: expanding the market. Elsevier, 2008. Cell Press, vol. 26:2, p. 100-108. (Link)
• GMO Compass, 2006: GM Microorganisms Taking the Place of Chemical Factories. (Link)
• Berg, J., Tymoczko, J.L., and Stryer, L.: Biochemistry, Seventh Edition. W.H.Freeman & Co Ltd, 2011.
• European Food Information Council, 1999: Lactic acid bacteria – their uses in food. (Link)
• Microbiology online: Bacteria. (Link)
• Gershon, E.: With you in the room, bacteria counts spike. Yale News, 2012. (Link)
• Nielsen , J.: Betydningen af systembiologi for industriel bioteknologi. Biozoom, 2007. Vol. 2, p. 1-3.(Link)
• Novo Nordisk: Use of gene technology at Novo Nordisk. (Link)
• Homepage of iGEM: Synthetic Biology – based on standard parts. (Link)

• WHO/FAO/UNU Expert Consultation: Protein and Amino Acid Requirements in Human Nutrition. United Nations University, 2002. No. 935, p. 164. (Link)