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Team:SDU-Denmark/Tour43
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| - | + | <h3>Fatty acids</h3> | |
<p> | <p> | ||
| - | + | We wanted to clone ∆9, ∆12 & ∆15 fatty acid desaturases into separately plasmids by the use of USER | |
| + | cloning. We wanted to use USER cloning to save some time and to ease the cloning process. Ahead of this | ||
| + | cloning, we needed the desaturases separately.<br><br> | ||
| + | |||
| + | We tried running several USER PCRs on ∆9 desaturase iGEM part but all were unsuccessful.<br><br> | ||
| + | |||
| + | We received the ∆12 desaurase (FAT-2) which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which | ||
| + | was successful. Thus we cloned it into a PSB1C3 plasmid and can now be found in parts registry under | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>(LINK). Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br> | ||
| + | |||
| + | We tried to run a colony PCR on the bacterium <i>Synechocystis sp.</i> PCC6803 in order for us to get the ∆15 | ||
| + | desaturase. Unfortunately, this was unsuccessful.<br><br> | ||
| + | |||
| + | After several failed attempts to run USER PCRs, we realized that we needed to prioritize and continue | ||
| + | without cloning ∆9, ∆12 & ∆15 into the separate plasmids, with the use of USER cloning. | ||
</p> | </p> | ||
Revision as of 12:19, 15 October 2014
Fatty acids
We wanted to clone ∆9, ∆12 & ∆15 fatty acid desaturases into separately plasmids by the use of USER
cloning. We wanted to use USER cloning to save some time and to ease the cloning process. Ahead of this
cloning, we needed the desaturases separately.
We tried running several USER PCRs on ∆9 desaturase iGEM part but all were unsuccessful.
We received the ∆12 desaurase (FAT-2) which originated from Caenorhabditis elegans. We ran a PCR which
was successful. Thus we cloned it into a PSB1C3 plasmid and can now be found in parts registry under
BBa_K1475002(LINK). Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.
We tried to run a colony PCR on the bacterium Synechocystis sp. PCC6803 in order for us to get the ∆15
desaturase. Unfortunately, this was unsuccessful.
After several failed attempts to run USER PCRs, we realized that we needed to prioritize and continue
without cloning ∆9, ∆12 & ∆15 into the separate plasmids, with the use of USER cloning.
