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Team:SDU-Denmark/Tour42
From 2014.igem.org
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| - | + | The pTet expression system and limonene synthase construct is evolved around one thing: the OneProt. | |
| + | We have made the pTet-OneProt construct in order for us to synthesize a nutritional protein with the | ||
| + | correct ratio between the essential amino acids and the correct ratio between essential and non-essential | ||
| + | amino acids in between. The device is found as <a href="http://parts.igem.org/Part:BBa_K1475000"> Bba_K1475000.</a><br><br> | ||
| + | |||
| + | The protein is self-designed, so we wanted to test if the protein were expressed in <i>E. coli</i> K12 MG1655, by | ||
| + | the use of Western Blotting. The western blot was blottet with <i>E. coli</i> K12 MG1655 wild-type and <i>E. coli</i> | ||
| + | expressing OneProt at different OD measures. | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | The protein has 3xFLAG tag and since bonds are showing, OneProt is expressed. However, from this | ||
| + | western blot, we cannot see if the protein has been cut, just that it is expressed. | ||
<br><br><br> | <br><br><br> | ||
</p> | </p> | ||
Revision as of 02:49, 17 October 2014
OneProt
The pTet expression system and limonene synthase construct is evolved around one thing: the OneProt.
We have made the pTet-OneProt construct in order for us to synthesize a nutritional protein with the
correct ratio between the essential amino acids and the correct ratio between essential and non-essential
amino acids in between. The device is found as Bba_K1475000.
The protein is self-designed, so we wanted to test if the protein were expressed in E. coli K12 MG1655, by
the use of Western Blotting. The western blot was blottet with E. coli K12 MG1655 wild-type and E. coli
expressing OneProt at different OD measures.
The protein has 3xFLAG tag and since bonds are showing, OneProt is expressed. However, from this
western blot, we cannot see if the protein has been cut, just that it is expressed.
