Team:SDU-Denmark/Tour46

From 2014.igem.org

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<font color="3397FE">"It always seems impossible until it's done." – <b>Nelson Mandela</b></font>
<font color="3397FE">"It always seems impossible until it's done." – <b>Nelson Mandela</b></font>
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<h1 class="groupTitel Bronze">5/5 Bronze Requirements</h1>
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<h1 class="groupTitel Bronze">6/6 Bronze Requirements</h1>
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   <p class="resultTitel">Team registration</p>
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   <p class="resultTitel">Register the team, have a great summer, and plan to have fun at the Giant Jamboree.</p>
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   <p class="resultText"><a class="dialogLink" href="https://igem.org/Team.cgi?id=1088">The team</a> applied for participation on the 26<sup>th</sup> of March and on the 12<sup>th</sup> of April we received the following message from the iGEM Headquarters: “Welcome to iGEM 2013 ! Your iGEM 2013 team application was accepted by iGEM Headquarters on 2013-04-12 13:07:54 and your team registration fee has been received.”</p>
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   <p class="resultText">Our team has been registered, we have had the best summer, and we sure plan to have lots of fun at the Giant Jamboree!</p>
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   <p class="resultTitel">Complete Judging form</p>
   <p class="resultTitel">Complete Judging form</p>
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   <p class="resultText">
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<a class="dialogLink" href="https://igem.org/2013_Judging_Form?id=1088">Our Judging form</a> was completed the 4<sup>th</sup> of October.</p>
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<a class="dialogLink" href="https://igem.org/2014_Judging_Form?id=1475">Our Judging form</a> was completed the 17<sup>th</sup> of October.</p>
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<div class="resultGroup">
<div class="resultGroup">
   <p class="resultTitel">Make a Team Wiki</p>
   <p class="resultTitel">Make a Team Wiki</p>
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   <p class="resultText">As you can hopefully see and feel, we have made a user friendly and intuitive wiki.</p>
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   <p class="resultText">We sure did, you are currently on it.</p>
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   <p class="resultTitel">Present a poster and a talk at the iGEM Jamboree</p>
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   <p class="resultTitel">Present a poster and a talk at the Giant iGEM Jamboree</p>
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   <p class="resultText">As of today (28<sup>th</sup> of october 2013) we are looking forward to and planning for a visit to the World Championship Jamboree in Boston to present our project for the other teams.</p>
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   <p class="resultText">We are looking forward and planning to attend the Gigant Jamboree in Boston, where we will present our project with a talk and a poster.</p>
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<div class="resultGroup">
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   <p class="resultTitel">Document a new standard BioBrick/Device used in your project</p>
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   <p class="resultTitel">Clearly attribute work done by the students and distinguish it from work done by others</p>
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   <p class="resultText">Our favorite Device this year is our <a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088016">BBa_K1088016</a>, because it seems likely that this Device makes our strain capable of producing rubber.</p>
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   <p class="resultText"> In the section; <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour14">Attributions</a> we describe all the help we have received and in the section; <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour30">Process</a> we describe how we divided the main responsibilities between us, team members.</p>
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<div class="resultGroup">
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  <p class="resultTitel">Document a new standard BioBrick/Device used in your project and submit it to the Registry</p>
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  <p class="resultText">Our BioBricks can be found in the section; <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour45">Submitted Parts</a>. Our favorite BioBrick is <a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475001">BBa_K1475001</a>, OneProt as basic part.</p>
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   <p class="resultTitel">Experimentally validate a BioBrick/Device of your own design and construction to work as expected</p>
   <p class="resultTitel">Experimentally validate a BioBrick/Device of your own design and construction to work as expected</p>
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   <p class="resultText">We have validated several of our BioBricks and Devices through experiments, see Submitted Parts for more info.</p>
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   <p class="resultText">We have validated that our BioBricks <a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475005">BBa_K1475005</a>, <a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475006">BBa_K1475006</a> and <a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475000">BBa_K1475000</a> work as expected.</p>
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<div class="resultGroup">
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   <p class="resultTitel">Document the characterization of this part</p>
   <p class="resultTitel">Document the characterization of this part</p>
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   <p class="resultText">All our characterization documentation has been uploaded to Parts Registry and much of it is available at our Characterization Results page.</p>
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   <p class="resultText">All our characterization documentation has been uploaded to Registry of Standard Biological Parts and is also available in our section; <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour40">Results</a>.</p>
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   <p class="resultTitel">Submit this new part to the iGEM Parts Registry</p>
   <p class="resultTitel">Submit this new part to the iGEM Parts Registry</p>
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   <p class="resultText">We have submitted several new parts to the Parts Registry, see <a href="https://2013.igem.org/Team:SDU-Denmark/Tour53">Submitted Parts</a> for more info.</p>
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   <p class="resultText">We have successfully submitted these <a href="https://2014.igem.org/Team:SDU-Denmark/Tour45">parts</a></p>
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   <p class="resultTitel">Describe one or more ways in which the environment, security, safety and ethics and/or ownership and sharing have been taken into consideration in the design and execution of your project</p>
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   <p class="resultTitel">Articulate at least one question encountered by your team beyond the bench, and describe how your team considered the(se) question(s) within your project</p>
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   <p class="resultText">Throughout this wiki, we hope to have made it clear what impact this project could have on the <a class="dialogLink" href="https://2013.igem.org/Team:SDU-Denmark/Tour23">environment</a>, the <a class="dialogLink" href="https://2013.igem.org/Team:SDU-Denmark/Tour34">safety</a> and the <a class="dialogLink" href="https://2013.igem.org/Team:SDU-Denmark/Tour63">ethics</a> of this project, and how we have taken this into consideration. </p>
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   <p class="resultText">Our major question is whether people will accept genetically modified microorganisms as a food source. To approach this, we have made a questionnaire, ethical considerations, a business plan, interviewed experts, made a wide outreach and an informative and interactive video adventure. For more details, visit our section; <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour50">Practices and Policy</a>. </p>
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<h1 class="groupTitel Gold">3/3 Gold Requirements</h1>
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<h1 class="groupTitel Gold">2/1 Gold Requirements</h1>
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<div class="resultGroup">
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   <p class="resultTitel">Improve the function of an existing BioBrick/Device</p>
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   <p class="resultTitel">Improve the function OR characterization of an existing BioBrick Part or Device</a>
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  <p class="resultText">We have improved the function of the LVA tagged LacI BioBrick <a class="dialogLink" href="http://parts.igem.org/Part:BBa_C0012">BBa_C0012</a>, by removing the LVA tag and submitted this part to the registry <a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1088018">BBa_K1088018.</a>
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<p class="resultText">We have improved the characterization of the TetR with LVA-tag, BioBrick <a class="dialogLink" href="http://parts.igem.org/Part:BBa_C0040">BBa_C0040</a>, by making an expression system with GFP and characterizing it by FACS (Fluorescence Acivated Cell Sorting) and SDS-PAGE (SDS Polyacrylamide Gel Electrophoresis) analysis. Furthermore we have improved the characterization of <a class="dialogLink" href="http://parts.igem.org/Part:BBa_J45999:Experience">BBa_J45999</a> by incorporating IMS (Iron Mobility Spectroscopy) analysis.</a>
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   <p class="resultTitel">Help another registered iGEM team in modeling or simulating their system</p>
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   <p class="resultTitel">Help another registered iGEM team</p>
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   <p class="resultText">We have helped the Edinburgh iGEM team with the modelling of their system. <a href="https://2013.igem.org/Team:Edinburgh/Collaboration" title="">Edinburgh's description of our mutually beneficial collaboration.</a></p>
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   <p class="resultText">We have helped the ETH-Zurich iGEM team and the Imperial College iGEM team. For more details, see <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour31">here</a></a>
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  <p class="resultTitel">Describe an approach that your team used to address at least one of these questions. Evaluate your approach, including whether it allowed you to answer your question(s), how it influenced the team’s scientific project, and how it might be adapted for others to use (within and beyond iGEM)</p>
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  <p class="resultText">We have described and evaluated our approaches at <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour50">Practices and Policy</a>.</a>
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<h3>iGEM Prizes</3>
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<h4>Best Policy & Practice advance</h4>
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<p>
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Our main goal was to investigate whether people would eat genetically modified bacteria. To do this, we have successfully accomplished to:
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<ul>
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<li>Make a <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour50">questionnaire</a>: we got answers from mainly 4 different countries (Denmark, UK, Argentina and Ghana)</li>
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<li><a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour51">Interview</a> experts in Ghana.</li>
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<li>Make a <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour53">business plan</a> to the implementation of our project.</li>
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<li>Make <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour52">ethical considerations</a> about GMOs as a food resource.</li>
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<li>Do a wide <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour54">outreach</a>.</li>
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<li>Make an informative and interactive <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour55">video adventure</a>.</li>
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</ul><br>
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<h4>Best supporting software</h4>
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We made a mockup to a <a class="dialogLink" href="https://2014.igem.org/Team:SDU-Denmark/Tour24#database">modelling database</a>. This database should help other teams with their modelling and ideally it should be included to the Registry, in order to make a "Registry of Modelling". One of the major time consumptions during this kind of modeling is finding rate equations and searching for parameters. This time consuming process reoccurs each year. We therefore propose that we make a common database for modelling, that is incorporated into the iGEM website.<br><br>
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<h4>Best new basic part</h4>
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<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475001">BBa_K1475001</a> and <a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475002">BBa_K1475002</a>
<br><br>
<br><br>
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<h4>Best new composit part</h4>
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<a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475006">BBa_K1475006</a> and <a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475000">BBa_K1475000</a>
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Latest revision as of 03:26, 18 October 2014

Judging criteria

"It always seems impossible until it's done." – Nelson Mandela

6/6 Bronze Requirements

Register the team, have a great summer, and plan to have fun at the Giant Jamboree.

Our team has been registered, we have had the best summer, and we sure plan to have lots of fun at the Giant Jamboree!

Complete Judging form

Our Judging form was completed the 17th of October.

Make a Team Wiki

We sure did, you are currently on it.

Present a poster and a talk at the Giant iGEM Jamboree

We are looking forward and planning to attend the Gigant Jamboree in Boston, where we will present our project with a talk and a poster.

Clearly attribute work done by the students and distinguish it from work done by others

In the section; Attributions we describe all the help we have received and in the section; Process we describe how we divided the main responsibilities between us, team members.

Document a new standard BioBrick/Device used in your project and submit it to the Registry

Our BioBricks can be found in the section; Submitted Parts. Our favorite BioBrick is BBa_K1475001, OneProt as basic part.

4/4 Silver Requirements

Experimentally validate a BioBrick/Device of your own design and construction to work as expected

We have validated that our BioBricks BBa_K1475005, BBa_K1475006 and BBa_K1475000 work as expected.

Document the characterization of this part

All our characterization documentation has been uploaded to Registry of Standard Biological Parts and is also available in our section; Results.

Submit this new part to the iGEM Parts Registry

We have successfully submitted these parts

Articulate at least one question encountered by your team beyond the bench, and describe how your team considered the(se) question(s) within your project

Our major question is whether people will accept genetically modified microorganisms as a food source. To approach this, we have made a questionnaire, ethical considerations, a business plan, interviewed experts, made a wide outreach and an informative and interactive video adventure. For more details, visit our section; Practices and Policy.

3/3 Gold Requirements

Improve the function OR characterization of an existing BioBrick Part or Device

We have improved the characterization of the TetR with LVA-tag, BioBrick BBa_C0040, by making an expression system with GFP and characterizing it by FACS (Fluorescence Acivated Cell Sorting) and SDS-PAGE (SDS Polyacrylamide Gel Electrophoresis) analysis. Furthermore we have improved the characterization of BBa_J45999 by incorporating IMS (Iron Mobility Spectroscopy) analysis.

Help another registered iGEM team

We have helped the ETH-Zurich iGEM team and the Imperial College iGEM team. For more details, see here

Describe an approach that your team used to address at least one of these questions. Evaluate your approach, including whether it allowed you to answer your question(s), how it influenced the team’s scientific project, and how it might be adapted for others to use (within and beyond iGEM)

We have described and evaluated our approaches at Practices and Policy.


iGEM Prizes

Best Policy & Practice advance

Our main goal was to investigate whether people would eat genetically modified bacteria. To do this, we have successfully accomplished to:


Best supporting software

We made a mockup to a modelling database. This database should help other teams with their modelling and ideally it should be included to the Registry, in order to make a "Registry of Modelling". One of the major time consumptions during this kind of modeling is finding rate equations and searching for parameters. This time consuming process reoccurs each year. We therefore propose that we make a common database for modelling, that is incorporated into the iGEM website.

Best new basic part

BBa_K1475001 and BBa_K1475002

Best new composit part

BBa_K1475006 and BBa_K1475000