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Team:SDU-Denmark/Tour43
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<h3>Fatty acids</h3> | <h3>Fatty acids</h3> | ||
<p> | <p> | ||
| + | <a class="popupImg alignRight" style="width:400px" target="_blank" href="https://static.igem.org/mediawiki/2014/5/53/2014SDUfattyacids2.png" title="Fish normaly provides a great amount of essential fatty acids."> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/9/92/2014SDUfattyacids1.png" style="width:400px" /> | ||
| + | Fish normaly provides a great amount of essential fatty acids. | ||
| + | </a> | ||
| + | |||
<span class="intro">We wanted to clone</span> ∆9, ∆12 & ∆15 fatty acid desaturases into separate plasmids by the use of USER | <span class="intro">We wanted to clone</span> ∆9, ∆12 & ∆15 fatty acid desaturases into separate plasmids by the use of USER | ||
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cloning, we needed the desaturases separately.<br><br> | cloning, we needed the desaturases separately.<br><br> | ||
| - | <span class="intro">We tried running several</span> | + | <span class="intro">We tried running several USER PCRs</span> on ∆9 desaturase iGEM part but all were unsuccessful.<br><br> |
| - | <span class="intro">We received the ∆12 | + | <span class="intro">We received the ∆12 desaturase (FAT-2)</span> which originated from <i>Caenorhabditis elegans</i>. We ran a PCR which was successful. Thus we cloned it into a PSB1C3 plasmid and it can now be found in parts registry under |
| - | was successful. Thus we cloned it into a PSB1C3 plasmid and can now be found in parts registry under | + | |
<a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br> | <a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a>. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.<br><br> | ||
| - | <span class="intro">We tried to run</span> | + | <span class="intro">We tried to run a colony PCR</span> on the bacterium <i>Synechocystis sp.</i> PCC6803, with Phusion polymerase, in order for us to get the ∆15 |
desaturase. Unfortunately, this was unsuccessful.<br><br> | desaturase. Unfortunately, this was unsuccessful.<br><br> | ||
Latest revision as of 03:27, 18 October 2014
Fatty acids
Fish normaly provides a great amount of essential fatty acids.
We wanted to clone ∆9, ∆12 & ∆15 fatty acid desaturases into separate plasmids by the use of USER
cloning. We wanted to use USER cloning to save some time and to ease the cloning process. Ahead of this
cloning, we needed the desaturases separately.
We tried running several USER PCRs on ∆9 desaturase iGEM part but all were unsuccessful.
We received the ∆12 desaturase (FAT-2) which originated from Caenorhabditis elegans. We ran a PCR which was successful. Thus we cloned it into a PSB1C3 plasmid and it can now be found in parts registry under
BBa_K1475002. Afterwards we tried running USER PCR on FAT-2 which was unsuccesful.
We tried to run a colony PCR on the bacterium Synechocystis sp. PCC6803, with Phusion polymerase, in order for us to get the ∆15
desaturase. Unfortunately, this was unsuccessful.
After several failed attempts to run USER PCRs, we realized that we needed to prioritize and continue
without cloning ∆9, ∆12 & ∆15 into the separate plasmids, with the use of USER cloning.
