Team:SDU-Denmark/Tour45

From 2014.igem.org

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<h3>Submitted parts</h3>
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<p>
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<span class="intro">Below is a list</span> of the parts we submitted to the parts registry. To see sequencing, characterization etc. click the part names to go to the parts registry page.<br><br>
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</p>
<p>
<p>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475001">BBa_K1475001</a></span><br>
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475001" target="_blank">BBa_K1475001</a></span><br>
<span class="intro">OneProt:</span>
<span class="intro">OneProt:</span>
The coding region of our self-designed nutritional protein. OneProt
The coding region of our self-designed nutritional protein. OneProt
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<br><br>
<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475002">BBa_K1475002</a></span><br>
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475002" target="_blank">BBa_K1475002</a></span><br>
<span class="intro">fat-2:</span>
<span class="intro">fat-2:</span>
This part contains only the coding region of the fat-2 desaturase from
This part contains only the coding region of the fat-2 desaturase from
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<br><br>
<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475003">BBa_K1475003</a></span><br>
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475003" target="_blank">BBa_K1475003</a></span><br>
<span class="intro">TetR(N):</span>
<span class="intro">TetR(N):</span>
the frequently used tet repressor from <i>Escherichia coli</i>
the frequently used tet repressor from <i>Escherichia coli</i>
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   <div class="accordionTitel">Regulatory devices</div>
   <div class="accordionTitel">Regulatory devices</div>
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   <div class="pane current">
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   <div class="pane">
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475004">BBa_K1475004</a></span><br>
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475004">BBa_K1475004</a></span><br>
<span class="intro">Pcons-TetR(N)-term:</span>
<span class="intro">Pcons-TetR(N)-term:</span>
the tet repressor from <i>Escherichia coli</i> is expressed
the tet repressor from <i>Escherichia coli</i> is expressed
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475008">BBa_K1475008</a></span><br>
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475008">BBa_K1475008</a></span><br>
<span class="intro">Pcon-LacI-term:</span>
<span class="intro">Pcon-LacI-term:</span>
Lac repressor protein derived from <i>Escherichia coli</i>
Lac repressor protein derived from <i>Escherichia coli</i>
without LVA degradation tag, controlled by a constitutively active promoter.
without LVA degradation tag, controlled by a constitutively active promoter.
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</div>
 
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<br>
 
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</div>
</div>
   <div class="accordionTitel">Constitutively active production devices</div>
   <div class="accordionTitel">Constitutively active production devices</div>
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   <div class="pane current">
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   <div class="pane">
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475000">BBa_K1475000</a></span><br>
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475000">BBa_K1475000</a></span><br>
<span class="intro">p(tetR)-OneProt-term:</span>
<span class="intro">p(tetR)-OneProt-term:</span>
OneProt is a self-designed protein designed to meet the
OneProt is a self-designed protein designed to meet the
recommended ratio of indispensible amino acids to a human diet according to  
recommended ratio of indispensible amino acids to a human diet according to  
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the world health organization (WHO). It also contains the recommended ration
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the world health organization (WHO). It also contains the recommended ratio
of indispensible to dispensible amino acids.
of indispensible to dispensible amino acids.
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 +
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</div>
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<div class="accordionTitel">Regulable production devices</div>
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  <div class="pane current">
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475005">BBa_K1475005</a></span><br>
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<span class="intro">Pcon-TetR(N)-term-p(TetR)-GFP-term:</span>
 +
GFP controlled by a TetR, pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet
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repressor protein is inhibited by aTc hereby inducing the expression of GFP.
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<br><br>
<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475009">BBa_K1475009</a></span><br>
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<span class="intro">P(TetR)-LIMS1-term:</span>
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475006">BBa_K1475006</a></span><br>
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Limonene synthase from <i>Citrus limon</i> regulated by
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<span class="intro">Pcon-TetR(LVA)-term-p(TetR)-GFP-term:</span>
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the tet promoter.
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GFP controlled by a TetR+LVA pTet
 +
expression system. The Tet repressor protein binds to the Tet promoter, the tet
 +
repressor protein is inhibited by aTc hereby inducing the expression of GFP.
<br><br>
<br><br>
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<span class="intro"><a class="dialogLink" href="http://parts.igem.org/Part:BBa_K1475010">BBa_K1475010</a></span><br>
 
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<span class="intro">P(TetR)-dxs-LIMS1-appY-term:</span>
 
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the limonene generator with tet promoter. The
 
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limonene generator consists of deoxyxylulose phosphtae synthase (dxs) from
 
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<i>Escherichia coli</i>, limonene synthase (LIMS1) from <i>Citrus limon</i>
 
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and appY from <i>Escherichia coli</i>.
 
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</p>
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<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475007">BBa_K1475007</a></span><br>
 +
<span class="intro">Pcon-LacI-term-p(LacI)-GFP-term:</span>
 +
GFP controlled by the lac repressor and lac promoter system.
 +
<br><br>
 +
 +
 +
<span class="intro"><a href="http://parts.igem.org/Part:BBa_K1475012">BBa_K1475012</a></span><br>
 +
<span class="intro">Pcon-LacI-p(LacI)-GFP-term:</span>
 +
GFP controlled by a LacI, p(LacI) system in which
 +
the LacI and p(Lac) sequence overlaps.
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</div>
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</div>
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</p>
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<br><br>
</html>
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{{:Team:SDU-Denmark/core/footer}}
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Latest revision as of 01:45, 18 October 2014

Edible coli
iGEM logo
Introduction
Edible coli
Process
Results
Policy and practices
Future

Submitted parts

Below is a list of the parts we submitted to the parts registry. To see sequencing, characterization etc. click the part names to go to the parts registry page.

Basic parts
BBa_K1475001
OneProt: The coding region of our self-designed nutritional protein. OneProt contains the recommended ratio of all the indispensible amino acids to a human diet. The protein is also compliant with the recommended ratio of indispensible to dispensable amino acids.

BBa_K1475002
fat-2: This part contains only the coding region of the fat-2 desaturase from Caenorhabditis elegans, the natural gene consists of three exons.

BBa_K1475003
TetR(N): the frequently used tet repressor from Escherichia coli (Part:BBa_C0040) modified by removing the LVA rapid degradation tag.
Regulatory devices
BBa_K1475004
Pcons-TetR(N)-term: the tet repressor from Escherichia coli is expressed form a constitutively active promoter. A terminator prevents transcription of genes downstream of the repressor.

BBa_K1475008
Pcon-LacI-term: Lac repressor protein derived from Escherichia coli without LVA degradation tag, controlled by a constitutively active promoter.
Constitutively active production devices
BBa_K1475000
p(tetR)-OneProt-term: OneProt is a self-designed protein designed to meet the recommended ratio of indispensible amino acids to a human diet according to the world health organization (WHO). It also contains the recommended ratio of indispensible to dispensible amino acids.
Regulable production devices
BBa_K1475005
Pcon-TetR(N)-term-p(TetR)-GFP-term: GFP controlled by a TetR, pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.

BBa_K1475006
Pcon-TetR(LVA)-term-p(TetR)-GFP-term: GFP controlled by a TetR+LVA pTet expression system. The Tet repressor protein binds to the Tet promoter, the tet repressor protein is inhibited by aTc hereby inducing the expression of GFP.

BBa_K1475007
Pcon-LacI-term-p(LacI)-GFP-term: GFP controlled by the lac repressor and lac promoter system.

BBa_K1475012
Pcon-LacI-p(LacI)-GFP-term: GFP controlled by a LacI, p(LacI) system in which the LacI and p(Lac) sequence overlaps.



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