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Team:SDU-Denmark/Tour33
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<h3>SOPs</h3> | <h3>SOPs</h3> | ||
<p> | <p> | ||
| - | <span class="intro">We used a lot | + | <span class="intro">We used a lot</span> |
| - | Standard Operating Procedures (SOPs) during our project written by last years SDU iGEM team. It is very important that every person in the lab uses the same SOP to make sure that the experiments does not depend on the person executing them, but only on the methods used. Any changes made to the SOPs were agreed | + | of different Standard Operating Procedures (SOPs) during our project written by last years SDU iGEM team. It is very important that every person in the lab uses the same SOP to make sure that the experiments does not depend on the person executing them, but only on the methods used. Any changes made to the SOPs were agreed upon by the entire team, to make sure that everyone knew when and why a Standard Operating Procedure was changed. |
Below you can explore all our SOPs: | Below you can explore all our SOPs: | ||
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/71/SDU2013_SOP0009_v01.pdf" target="_blank">iGEM2013 SOP0009 v01 - TSB transformation</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/71/SDU2013_SOP0009_v01.pdf" target="_blank">iGEM2013 SOP0009 v01 - TSB transformation</a><br> | ||
<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/9/93/SDU2013_SOP0010_v01.pdf" target="_blank">iGEM2013 SOP0010 v01 - Phusion PCR</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/9/93/SDU2013_SOP0010_v01.pdf" target="_blank">iGEM2013 SOP0010 v01 - Phusion PCR</a><br> | ||
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/74/SDU2013_SOP0012_v01.pdf" target="_blank">iGEM2013 SOP0012 v01 - Restriction Digest</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/74/SDU2013_SOP0012_v01.pdf" target="_blank">iGEM2013 SOP0012 v01 - Restriction Digest</a><br> | ||
<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/c/cd/SDU2013_SOP0013_v01.pdf" target="_blank">iGEM2013 SOP0013 v01 - Nano-drop</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/c/cd/SDU2013_SOP0013_v01.pdf" target="_blank">iGEM2013 SOP0013 v01 - Nano-drop</a><br> | ||
<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_SOP0014_v01.pdf" target="_blank">iGEM2013 SOP0014 v01 - Gel purification</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_SOP0014_v01.pdf" target="_blank">iGEM2013 SOP0014 v01 - Gel purification</a><br> | ||
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/2/2c/SDU2013_SOP0016_v01.pdf" target="_blank">iGEM2013 SOP0016 v01 - LB and LA media</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/2/2c/SDU2013_SOP0016_v01.pdf" target="_blank">iGEM2013 SOP0016 v01 - LB and LA media</a><br> | ||
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/ed/SDU2013_SOP0018_v01.pdf" target="_blank">iGEM2013 SOP0018 v01 - Agar plates with Cm</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/ed/SDU2013_SOP0018_v01.pdf" target="_blank">iGEM2013 SOP0018 v01 - Agar plates with Cm</a><br> | ||
<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/85/SDU2013_SOP0019_v01.pdf" target="_blank">iGEM2013 SOP0019 v01 - Plasmid Miniprep</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/85/SDU2013_SOP0019_v01.pdf" target="_blank">iGEM2013 SOP0019 v01 - Plasmid Miniprep</a><br> | ||
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/1/15/SDU2013_SOP0021_v01.pdf" target="_blank">iGEM2013 SOP0021 v01 - MyTaq Colony PCR</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/1/15/SDU2013_SOP0021_v01.pdf" target="_blank">iGEM2013 SOP0021 v01 - MyTaq Colony PCR</a><br> | ||
<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/5/56/SDU2013_SOP0022_v01.pdf" target="_blank">iGEM2013 SOP0022 v01 - Speedy Vac</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/5/56/SDU2013_SOP0022_v01.pdf" target="_blank">iGEM2013 SOP0022 v01 - Speedy Vac</a><br> | ||
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/4/4e/SDU2013_SOP0024_v01.pdf" target="_blank">iGEM2013 SOP0024 v01 - Growth experiment</a><br> | <a class="pdfFile" href="https://static.igem.org/mediawiki/2013/4/4e/SDU2013_SOP0024_v01.pdf" target="_blank">iGEM2013 SOP0024 v01 - Growth experiment</a><br> | ||
| - | <a class="pdfFile" href="https://static.igem.org/mediawiki/ | + | <a class="pdfFile" href="https://static.igem.org/mediawiki/2014/4/44/IGEM2014_SOP0011_v01_WesternBlotting.pdf" target="_blank">iGEM2014 SOP0011 v01 - WesternBlotting</a><br> |
| - | <a class="pdfFile" href="https://static.igem.org/mediawiki/ | + | <a class="pdfFile" href="https://static.igem.org/mediawiki/2014/e/e6/IGEM2014_SOP0015_v02_Ligation.pdf" target="_blank">iGEM2014 SOP0015 v02 - Ligation</a><br> |
| - | <a class="pdfFile" href="https://static.igem.org/mediawiki/ | + | <a class="pdfFile" href="https://static.igem.org/mediawiki/2014/c/c4/IGEM2014_SOP0017_v01_Fast_digest.pdf" target="_blank">iGEM2014 SOP0017 v01 - Fast digest</a><br> |
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Latest revision as of 21:42, 17 October 2014
SOPs
We used a lot
of different Standard Operating Procedures (SOPs) during our project written by last years SDU iGEM team. It is very important that every person in the lab uses the same SOP to make sure that the experiments does not depend on the person executing them, but only on the methods used. Any changes made to the SOPs were agreed upon by the entire team, to make sure that everyone knew when and why a Standard Operating Procedure was changed.
Below you can explore all our SOPs:
iGEM2013 SOP0001 v01 - ON culture of E. coli
iGEM2013 SOP0002 v01 - Table autoclave
iGEM2013 SOP0003 v01 - Bacterial freezing stock
iGEM2013 SOP0004 v01 - Primer Design
iGEM2013 SOP0005 v01 - USER primer design
iGEM2013 SOP0006 v01 - PCR protocol for USER cloning
iGEM2013 SOP0007 v01 - excision and ligation of PCR product in USER cloning
iGEM2013 SOP0008 v01 - preparing primers
iGEM2013 SOP0009 v01 - TSB transformation
iGEM2013 SOP0010 v01 - Phusion PCR
iGEM2013 SOP0012 v01 - Restriction Digest
iGEM2013 SOP0013 v01 - Nano-drop
iGEM2013 SOP0014 v01 - Gel purification
iGEM2013 SOP0016 v01 - LB and LA media
iGEM2013 SOP0018 v01 - Agar plates with Cm
iGEM2013 SOP0019 v01 - Plasmid Miniprep
iGEM2013 SOP0020 v01 - Agarose gel DNA
iGEM2013 SOP0021 v01 - MyTaq Colony PCR
iGEM2013 SOP0022 v01 - Speedy Vac
iGEM2013 SOP0024 v01 - Growth experiment
iGEM2014 SOP0011 v01 - WesternBlotting
iGEM2014 SOP0015 v02 - Ligation
iGEM2014 SOP0017 v01 - Fast digest
