Team:SDU-Denmark/Tour54

From 2014.igem.org

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<html>
 
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<h3> Manuscript </h3>
 
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<html>
 +
<h3> Sharing the message </h3>
 +
<p class='intro'>
 +
<font color="3397FE">"When you can't make them see the light, make them feel the heat." – <b>Ronald Reagan</b></font>
 +
</p>
<p>
<p>
 +
<span class="intro">To present a scientific project</span> is very educational. It requires a good understanding of the project and might lead to a better understanding of the details. Furthermore response and questions from listeners can be very helpful, as they give an idea of the understanding of the subject as well as show new aspects and reflections on the project.<br>
 +
We think that it is important to practice presentation skills and use all the benefits a presentation might lead to. Therefore we participated in different outreach events, where all team members have practiced their individual presentation skills at least once.<br>
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We spend much time on outreach, but we can conclude that the benefit, especially in the form of response, had a big impact on our project.
 +
</p>
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<i> <b> Boy walks into a burger joint and orders a cheeseburger. </b></i>
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<br>
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<table style="width:800px">
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<a class="popupImg alignRight" style="width:150px" target="_blank" href="https://static.igem.org/mediawiki/2014/d/d4/2014SDUevents4.jpg" title="iGEM meet-up in London.">
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  <tr>
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  <img src="https://static.igem.org/mediawiki/2014/d/d4/2014SDUevents4.jpg" style="width:150px" />
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    <td style="width:50px">1.</td>
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iGEM meet-up in London.
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    <td style="width:400px"> <b>Burger with or without bacteria? </b></td>
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</a>
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  </tr>
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-
  <tr>
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    <td style="width:50px"> </td>
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-
    <td style="width:400px">
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<u>Burger mama:</u> <br>
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”Hello there, kid.” <br> <br>
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<u> Boy: </u><br>
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<h4>iGEM meet-up in London hosted by YSB (1<sup>st</sup> of September - 2<sup>nd</sup> of September)</h4>
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”Hello there, burger mama.” <br><br>
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<p>
 +
<span class="intro">We were very happy to participate in</span> the iGEM meet-up in London. Here we got the first opportunity to tell others about our project and discuss our policies and practices with other iGEM teams. Additionally, there were a lot of friendly participants who helped by filling out our questionnaire and giving us valuable feedback.
 +
</p>
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<u>Burger mama:</u> <br>
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<br>
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”What can I do for you today, mister?”
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<u> Boy: </u><br>
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<h4>Study Trade Fair (11<sup>th</sup> of September)</h4>
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”I would like one juicy cheeseburger, please.” <br><br>
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<p>
 +
<a class="popupImg alignRight" style="width:150px" target="_blank" href="https://static.igem.org/mediawiki/2014/d/d5/2014SDUevents6.jpg" title="Study Trade Fair.">
 +
  <img src="https://static.igem.org/mediawiki/2014/1/15/2014SDUevents9.PNG" style="width:150px" />
 +
Study Trade Fair.
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</a>
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<u>Burger mama:</u> <br>
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<span class="intro">We participated in the annual</span> Study Trade Fair at our university to raise awareness about the iGEM competition, our project, promote our outreach events in October, to get more answers to our questionnaire and to promote the recruiting for next year’s iGEM-team. The day was a great success. We met many interested students who wanted to hear more about iGEM in general and about our project. In addition, we were able to practice our presentation skills.
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”Okay then, coming right up. Do you want it with or without bacteria?” <br><br>
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</p>
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<i> (Boy looks confused into camera – CHOICE: 1.1 or 1.2) </i> <br><br></td>
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<br>
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  </tr>
+
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<tr>
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<h4>Old-iGEM Meet-up (18<sup>th</sup> of September)</h4>
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    <td style="width:50px">1.1</td>
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<a class="popupImg alignRight" style="width:150px" target="_blank" href="https://static.igem.org/mediawiki/2014/5/58/2014SDUevents7.PNG" title="Old-GEM meet-up.">
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    <td style="width:400px"> <b>With: </b></td>
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  <img src="https://static.igem.org/mediawiki/2014/3/34/2014SDUevents13.PNG" style="width:150px" />
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</tr>
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Old-GEM meet-up.
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<tr>
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</a>
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<u>Burger mama:</u> <br>
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-
”Well, I couldn’t really prevent your burger from containing bacteria either, since there are
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bacteria everywhere. If we imagined a bacteria free or sterile burger, this would be ruined
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already after your first touch and breath on it, since bacteria is a big and important part of
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the environment on your skin and in your 
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-
<span class="sourceReference">mouth</span>
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<span class="tooltip">
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-
  <span class="tooltipHeader">Source:</span>
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    Microbiology online: Bacteria.
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<a href="http://www.microbiologyonline.org.uk/about-microbiology/introducing-microbes/bacteria" target="_blank">(Link)</a>
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<p>
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</span>.
+
-
<br><br>
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In addition to this, you would have to lose the cheese in cheeseburger. The cheesemaking
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process, apparently, involves fermentation of milk, which is performed by lactic acid
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bacteria. These converts milk sugar into lactic acid. When the lactic acid accumulates, the
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structure of milk proteins changes and this causes texture of the <span class="sourceReference">product.</span>
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<span class="tooltip">
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  <span class="tooltipHeader">Source:</span>
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    European Food Information Council, 1999: Lactic acid bacteria – their uses in food.
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<a href="http://www.eufic.org/article/en/artid/lactic-acid-bacteria/" target="_blank">(Link)</a>
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<span class="intro">We invited all former SDU-iGEM participants</span>
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</span><br><br>  
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to a meet up… and it was a success! They heard about our project and responded to our presentation with valuable feedback. Our supervisors had arranged and prepared dinner after our presentation, which gave us a chance to discuss the responses. Thank you to all of them for coming and making this a cozy evening.<br>
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At least somebody told me so.” <br> <br>
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</p>
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<u>Boy:</u> <br>
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<br>
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”I could never lose the cheese!”<br><br>
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<i>(Boy gets burger) </i><br><br>
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<i> To 1.1.1 </i><br><br>
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<h4>Bioscientific dissemination (30<sup>th</sup> of September)</h4>
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</td>
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<p>
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  </tr>
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<span class="intro">Bioscientific dissemination is an event</span> for students by students. It is an event where
-
<tr>
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students get the chance to present their individual, bachelor and master projects to get a great response
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    <td style="width:50px">1.2</td>
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and practice presentation skills. The iGEM SDU-Denmark 2014 project was presented by one of our team members, and he got constructive
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    <td style="width:400px"> <b>Without: </b></td>
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criticism as well as a great experience.
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  </tr>
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</p>
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  <tr>
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<br>
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<u>Burger mama:</u> <br>
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”Well, we just give it some time in the autoclave then, to sterilize this bad boy.”<br><br>
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<i> (Autoclaving burger) </i> <br><br>
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<i> (Boy grabs burger and reaches his mouth with it – Burger mama takes it out of his hands
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instantly) </i> <br><br>
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”Well, you can’t eat this now, since you contaminated it with your hands and breath.” <br><br>
+
-
<i>(*BOOM* to the classroom)</i> <br><br>
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<u>Professor:</u> <br>
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”Did you know that bacteria are found everywhere. Relatively few are bad for your health
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and can cause diseases – most are thought to be harmless and some even necessary for
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your body’s function. Bacteria are considered microorganisms and can’t be seen with the
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human eye. The human body consists of approximately 10 times as many bacterial cells as
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human cells – many of these are found lining the gastrointestinal tract. The surface of the
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human body, like the intestines, mouth and skin, is thus covered inside and out with millions
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of microorganisms, known as our normal body flora, which in fact helps to protect us from
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infectious
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<span class="sourceReference">microorganisms</span>
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<span class="tooltip">
+
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  <span class="tooltipHeader">Source:</span>
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    Microbiology online: Bacteria.
+
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<a href="http://www.microbiologyonline.org.uk/about-microbiology/introducing-microbes/bacteria" target="_blank">(Link)</a>
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<h4>Sundhedsmekka: An exhibition for medical students (1<sup>st</sup> of October)</h4>
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</span>.<br><br>
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<p>
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At the same time, the air we breathe is full of bacteria as well. Largely by stirring up material
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<a class="popupImg alignRight" style="width:300px" target="_blank" href="https://static.igem.org/mediawiki/2014/5/51/2014SDUevents14.jpg" title="Sundhedsmekka.">
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from the floor, left by previous persons’ attendance, a person can add about 37 million
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  <img src="https://static.igem.org/mediawiki/2014/5/51/2014SDUevents14.jpg" style="width:300px" />
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bacteria to the air every hour, just by his or her mere presence in a
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Sundhedsmekka.
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<span class="sourceReference">room</span>
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</a>
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<span class="tooltip">
+
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  <span class="tooltipHeader">Source:</span>
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-
    Gershon, E.: With you in the room, bacteria counts spike. Yale News, 2012.
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<a href="http://news.yale.edu/2012/03/28/you-room-bacteria-counts-spike" target="_blank">(Link)</a>
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-
</span>.<br><br>
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In addition to this, the manufacturing of many food products involves the activity of different
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microorganisms. Cheesemaking involves fermentation of milk by lactic acid bacteria, and a
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yeast called <i>Saccharomyces cerevisiae</i> causes the expansion of dough for making bread, by
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its fermentation of sugar to
+
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<span class="sourceReference">carbon oxide gas</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
    European Food Information Council, 1999: Lactic acid bacteria – their uses in food.
+
-
<a href="http://www.eufic.org/article/en/artid/lactic-acid-bacteria/" target="_blank">(Link)</a>
+
-
</span>.<br><br>
+
-
So for instance this burger, you were just trying to buy, could never be made without
+
-
containing bacteria.” <br><br>
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<u>Boy:</u> <br>
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<span class="intro">Sundhedsmekka is a yearly event,</span>
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”Oh, nasty shit. But I understand – bacteria are kind of awesome then!”<br><br>
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where associations and event groups with interests that concerns medical students can promote themselves. Originally, it was a medical student who started the first iGEM team from SDU. Since then the iGEM teams representing SDU have at least had one medical student on their team. In spite of this, most medical students at SDU have not heard of iGEM, and even synthetic biology is an unfamiliar field to most medical students.
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<i>(*BOOM* to burger joint)</i> <br><br>
+
By participating in Sundhedsmekka our iGEM team got a chance to explain the concept of the iGEM contest and our own project. It was a great success to talk to all the many interested students and in connection with the conversations; we invited people to attend our “Quiz and Presentation night”, which had premiere in the following week.
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<i> To 1.2.1 </i><br><br>
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-
</td>
+
-
</tr>
+
-
<tr>
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</p>
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    <td style="width:50px">1.1.1</td>
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    <td style="width:400px"> <b>French fries with or without DNA?* </b></td>
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-
  </tr>
+
-
  <tr>
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<u>Boy:</u> <br>
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”Oh, burger mama. I forgot to order some french fries with my burger.”<br><br>
+
-
<u>Burger mama:</u> <br>
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<br>
-
”Coming up, boy. But do you want them with or without DNA?”<br><br>
+
-
<i>(Boy looks confused into camera – CHOICE: 1.1.2 or 1.1.3)</i> <br><br>
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<h4>Quiz and Presentation night at the Student House (6<sup>th</sup> of October)</h4>
 +
<p>
 +
<a class="popupImg alignRight" style="width:150px" target="_blank" href="https://static.igem.org/mediawiki/2014/1/17/2014SDUevents8.jpg" title="Quiz and Presentation night at the Student House.">
 +
  <img src="https://static.igem.org/mediawiki/2014/3/3c/2014SDUevents10.PNG" style="width:150px" />
 +
Quiz and Presentation night at the Student House.
 +
</a>
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</td>
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<span class="intro"> The Student House is a gathering place</span> for students and locals, which is located in the center of Odense.
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</tr>
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The purpose of the Student House is to have a forum where university students can come to study or to  
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take part in social events. Volunteers are responsible for the café area, where different events
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<tr>
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take place frequently. Our team arranged a Microorganism Quiz and Presentation Night at the Student
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    <td style="width:50px">1.1.2</td>
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House where around fifty people participated. Our presentation was about the main concepts of iGEM and
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    <td style="width:400px"> <b>With: </b></td>
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synthetic biology and additionally an overview of our own project.
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  </tr>
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The presentation was followed by our quiz about microorganisms. Every question had a preamble
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  <tr>
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concerning microorganisms but the question usually was a digression from the subject. Our idea with the  
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    <td style="width:50px"> </td>
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event was that the questions should apply to anyone and it ended up being a great success for this reason.<br><br>
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    <td style="width:400px">
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</p>
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<u>Burger mama:</u> <br>
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”Well, good choice there boy. I’m not sure I really could make you any french fries without
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DNA, since french fries are made of potatoes and potatoes are tubers from a tuberous crop.
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Plants consist of cells and every cell contains DNA, which makes french fries full of DNA, I
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think?!”<br><br>
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<i>(Burger mama shows french frie → potato → potato plant)</i><br><br>
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-
 
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<u>Boy:</u> <br>
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”Yeah, that’s what I thought too.”<br><br>
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<i>(Boy gets french fries)</i> <br><br>
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-
 
+
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<u>Burger mama:</u> <br>
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”But kid, can you tell me what exactly DNA is?”<br><br>
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<i>(Boy looks confused into camera – CHOICE – 1.1.2.1 or 1.1.2.1)</i><br><br>
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-
 
+
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</td>
+
-
</tr>
+
-
 
+
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<tr>
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    <td style="width:50px">1.1.2.1</td>
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    <td style="width:400px"> <b>Yes: </b></td>
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  </tr>
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  <tr>
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<u>Boy:</u> <br>
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”DNA stands for deoxyribonucleic acid. It is a macromolecule found in every cell, forming
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a living organism. DNA encodes genes, a given cell and organism’s characteristics, by the
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sequence of four different bases; Adenine, Thymine, Guanine and Cytosine. It’s also the
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genetic inheritance of every cell and living organism, since these genes can be passed on to
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the next
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<span class="sourceReference">generation</span>
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<span class="tooltip">
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  <span class="tooltipHeader">Source:</span>
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    Berg, J., Tymoczko, J.L., and Stryer, L.: Biochemistry, Seventh Edition.
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W.H.Freeman & Co Ltd, 2011
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</span>.”<br><br>
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<u>Burger mama:</u> <br>
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”Well, that is truly awesome! You’re a clever fella, huh?!”<br><br>
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-
<i> To 2.</i><br><br>
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-
 
+
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</td>
+
-
</tr>
+
-
 
+
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<tr>
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    <td style="width:50px">1.1.2.2</td>
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    <td style="width:400px"> <b>No: </b></td>
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  </tr>
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-
  <tr>
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<i>(*BOOM* to the classroom)</i><br><br>
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<u>Professor:</u> <br>
+
-
”Did you know that DNA stands for deoxyribonucleic acid? It’s a macromolecule, found
+
-
in every cell of every living organism. It consists of sugar, phosphate and four different
+
-
bases; Adenine, Thymine, Guanine and Cytosine. These four bases are found bound to each
+
-
other in pairs; A-T and G-C. The DNA molecule is shaped like a double helix, in which two
+
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phosphorous sugar backbones are held together by the base pairs, like steps on a ladder. The
+
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sequence of the bases encodes genes, the specific cell and organism’s characteristics, which
+
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can be inherited to the next
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<span class="sourceReference">generation</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
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-
    Berg, J., Tymoczko, J.L., and Stryer, L.: Biochemistry, Seventh Edition.
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-
W.H.Freeman & Co Ltd, 2011
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-
</span>.<br><br>
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So since most food products are made from living organisms, all vegetables and meat for
+
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instance, many food products will contain DNA. It would be impossible to avoid eating DNA.”<br><br>
+
-
 
+
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<u>Boy:</u> <br>
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”Cool!”<br><br>
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<i>(*BOOM* to burger joint)</i><br><br>
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<i>To 2. </i> <br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
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    <td style="width:50px">1.1.3</td>
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    <td style="width:400px"> <b>Without: </b></td>
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  </tr>
+
-
  <tr>
+
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    <td style="width:50px"> </td>
+
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    <td style="width:400px">
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<u>Burger mama:</u> <br>
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”But why not?”<br><br>
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<i>(Boy looks confused into camera – CHOICE: 1.1.3.1, 1.1.3.2, 1.1.3.3 or 1.1.3.4)</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
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    <td style="width:50px">1.1.3.1</td>
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    <td style="width:400px"> <b>Unhealthy: </b></td>
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  </tr>
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-
  <tr>
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<u>Burger mama:</u> <br>
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”I don’t think that juicy cheeseburger is any better.”<br><br>
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<i> To 1.1.3.5 </i> <br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
 
+
-
<tr>
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    <td style="width:50px">1.1.3.2</td>
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    <td style="width:400px"> <b>Disgusting: </b></td>
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  </tr>
+
-
  <tr>
+
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<u>Burger mama:</u> <br>
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”Well, you’re a picky young man, huh?!”<br><br>
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<i> To 1.1.3.5 </i> <br><br>
+
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</td>
+
-
</tr>
+
-
 
+
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<tr>
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    <td style="width:50px">1.1.3.3</td>
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    <td style="width:400px"> <b>Dangerous: </b></td>
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  </tr>
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-
  <tr>
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<u>Burger mama:</u> <br>
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”Wopwopwop, I don’t really believe that’s entirely true.”<br><br>
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<i> To 1.1.3.5 </i> <br><br>
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-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
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-
    <td style="width:50px">1.1.3.4</td>
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    <td style="width:400px"> <b>Don't know?: </b></td>
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  </tr>
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-
  <tr>
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<u>Burger mama:</u> <br>
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”Young man, you can’t go on in life, being so indecisive and indifferent. Just some useful
+
-
advice from your old burger mama.”<br><br>
+
-
<i> To 1.1.3.5 </i> <br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">1.1.3.5</td>
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    <td style="width:400px"> <b>DNA </b></td>
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  </tr>
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  <tr>
+
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    <td style="width:50px"> </td>
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    <td style="width:400px">
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<i>(*BOOM* to the classroom)</i><br><br>
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-
<u>Professor:</u> <br>
+
-
”Did you know that every living organism consists of one or more cells, and that every cell
+
-
contains DNA?<br><br>
+
-
DNA stands for deoxyribonucleic acid. It is a macromolecule consisting of sugar, phosphate
+
-
and four different bases; Adenine, Thymine, Guanine and Cytosine. These four bases are
+
-
found bound to each other in pairs; A-T and G-C. The DNA molecule is shaped like a double
+
-
helix, in which two phosphorous sugar backbones are held together by the base pairs, like
+
-
steps on a ladder. The sequence of the bases encodes genes, the specific cell and organism’s
+
-
characteristics, which can be inherited to the next
+
-
<span class="sourceReference">generation</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
    Berg, J., Tymoczko, J.L., and Stryer, L.: Biochemistry, Seventh Edition.
+
-
W.H.Freeman & Co Ltd, 2011
+
-
</span>.<br><br>
+
-
So these french fries, you were just trying to buy, actually contain a whole lot of DNA, like
+
-
most other food products, all vegetables and meat for instance. French fries are made of
+
-
potatoes and potatoes are tubers of a potato plant. Plants are considered living organisms,
+
-
and thus consists of cells that all contain DNA. It would be impossible for you to order french
+
-
fries without DNA.”<br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Oh! I see. DNA doesn’t do any harm. I would like some french fries anyways then.”<br><br>
+
-
 
+
-
<i>(*BOOM* back to burger joint. Boy gets french fries)</i><br><br>
+
-
 
+
-
<u>Burger mama:</u> <br>
+
-
”There you go, boy.”<br><br>
+
-
 
+
-
<i> To 2</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">1.2.1</td>
+
-
    <td style="width:400px"> <b>New burger? </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Burger mama:</u> <br>
+
-
”Would you like a new burger? – You will have to pay double though, of course.”<br><br>
+
-
 
+
-
<i>(Boy looks confused into camera – CHOICE: 1.2.2 or 1.2.3)</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">1.2.2</td>
+
-
    <td style="width:400px"> <b>Yes: </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Burger mama:</u> <br>
+
-
”Sensible decision, kid. It would have been absolutely gross, if you decided to be a stingy fella
+
-
and eat this nasty bastard.”<br><br>
+
-
 
+
-
<i>(Boy gets new burger)</i><br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Yeah, this is better!”<br><br>
+
-
 
+
-
<i> To 1.1.1*</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">1.2.3</td>
+
-
    <td style="width:400px"> <b>No: </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Burger mama:</u> <br>
+
-
”Okay then. That’s your decision. I’m just glad it isn’t me who have to eat that nasty
+
-
bastard!”<br><br>
+
-
 
+
-
<i>(Boy touches burger and throws it out)</i><br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Oh, I won’t eat it either. This is a flabby excuse for a cheeseburger. I changed my mind,
+
-
burger mama – can you get me a new one, please. Extra cheese then, if I have to pay
+
-
double!”<br><br>
+
-
 
+
-
<u>Burger mama:</u> <br>
+
-
”All right, kid. That’s a deal.”<br><br>
+
-
 
+
-
<i>(Boy gets burger)</i><br><br>
+
-
 
+
-
<i> To 1.1.1*</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">2.</td>
+
-
    <td style="width:400px"> <b>GMO </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<i>(Scared woman runs past boy and burger mama in burger joint)</i><br><br>
+
-
<u>Scared woman:</u> <br>
+
-
”Save me, save me. Save all of us! The GMO is coming. It’s following me. It’s taking me.
+
-
Aarrh!”<br><br>
+
-
 
+
-
<i>(Scared woman disappears/runs out of burger joint again. Burger mama looks confused at
+
-
boy)</i> <br><br>
+
-
 
+
-
<u>Burger mama:</u> <br>
+
-
”What the f***! That was weird. I don’t even know what this GMO is. Do you?”<br><br>
+
-
 
+
-
<i>(Boy looks confused into camera – CHOICE: 2.1 or 2.2)</i> <br><br>
+
-
 
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">2.1</td>
+
-
    <td style="width:400px"> <b>Yes: </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Sure. GMO stands for Genetically Modified Organism. Genetic modification can serve to  
+
-
induce several new qualities into an organism by introducing new genes to its native DNA
+
-
material. Genetic modification is for instance used on crops in farming to ensure a high yield
+
-
production. GMOs also include microorganisms. Even if genetically modified microorganisms
+
-
were to escape into nature, they can be programmed with sophisticated <i>kill switches</i>, making
+
-
them unfit to survive outside a controlled environment. Ironically, different microorganisms
+
-
produce antibiotics by fermentation and are therefore used in the medical industry – these
+
-
microorganisms are then genetically modified to yield maximum
+
-
<span class="sourceReference">production</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
    Nielsen , J.: Betydningen af systembiologi for industriel bioteknologi. Biozoom, 2007. Vol. 2, p. 1-3.
+
-
<a href="http://www.biokemi.org/biozoom/issues/514/articles/2284" target="_blank">(Link)</a>
+
-
</span>.”<br><br>
+
-
 
+
-
<u>Burger mama:</u> <br>
+
-
”Kid, you really amaze me with all that brain juice flowing from you!”<br><br>
+
-
 
+
-
<i>To 3</i> <br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">2.2</td>
+
-
    <td style="width:400px"> <b>No: </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<i>(*BOOM* to the classroom)</i><br><br>
+
-
<u>Professor:</u> <br>
+
-
”Did you know that GMO stands for Genetically Modified Organism? Genetic modification
+
-
is a gene technology, which is used to change the genetics and thus the properties of an
+
-
organism, either by removing genes or introducing new genes to its native DNA material.  
+
-
In this way, genetic modification can be used to induce several new characteristics into an
+
-
organism, for instance crops in farming, to ensure a high yield
+
-
<span class="sourceReference">production</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
    GMO Compass, 2006: GM Microorganisms Taking the Place of Chemical Factories.
+
-
<a href="http://www.gmo-compass.org/eng/grocery_shopping/ingredients_additives/36.gm_microorganisms_taking_place_chemical_factories.html" target="_blank">(Link)</a>
+
-
</span>.<br><br>
+
-
GMOs can also be microorganisms. Even if genetically modified microorganisms were to
+
-
escape into nature, they can be programmed with sophisticated <i>kill switches</i>, making them
+
-
unfit to survive outside a controlled environment. Genetically modified microorganisms
+
-
are especially used in the medical industry. Different microorganisms produce antibiotics
+
-
by fermentation – these microorganisms are then genetically modified to yield maximum
+
-
production. Novo Nordisk, a Danish pharmaceutical company, also uses a modified fungus,
+
-
the <i>Aspergillus oryzae</i>, in the production of their main commodity;
+
-
<span class="sourceReference">insulin</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
    Novo Nordisk: Use of gene technology at Novo Nordisk.
+
-
<a href="http://www.novonordisk.com/old/press/environmental/er98/bioethics/useofgenetechnol.html" target="_blank">(Link)</a>
+
-
</span>.”<br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Wow. I actually got diabetes – so I’m kind of fond of the genetically modified
+
-
microorganisms at the moment!”<br><br>
+
-
 
+
-
<i>(*BOOM* to burger joint)</i><br><br>
+
-
 
+
-
<i>To 3</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">3.</td>
+
-
    <td style="width:400px"> <b>Lemonade produced by GMO? </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Boy:</u> <br>
+
-
”Burger mama, I think I got some thirst going on. Can you hand me a lemonade as well,
+
-
please?”<br><br>
+
-
 
+
-
<u>Burger mama:</u> <br>
+
-
”Of course, boy. But can your lemonade be produced by GMO?<br><br>
+
-
 
+
-
<i>(Boy looks confused into camera – CHOICE: 3.1 or 3.2)</i><br><br>
+
-
 
+
-
 
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">3.1</td>
+
-
    <td style="width:400px"> <b>Yes: </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Boy:</u> <br>
+
-
”Well, since GMOs can produce antibiotics, they could as well produce my lemonade, I
+
-
guess. I think I’ve heard that genetically modified microorganisms produce lots of food
+
-
additives, vitamins and flavors as well. I see lemonade contains the additive E330, citric acid,
+
-
which is part of many microorganisms metabolic pathway. To make an economically viable
+
-
production, a given microorganism should just be modified for an optimized metabolic flux of  
+
-
the
+
-
<span class="sourceReference">citric acid?</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
    Sauer, M., et al.: Microbial production of organic acids: expanding the market. Elsevier, 2008. Cell Press, vol. 26:2, p. 100-108.
+
-
<a href="http://awe.mol.uj.edu.pl/~allel/s6/pliki/mbPrz_seminaria/microbial%20production.pdf" target="_blank">(Link)</a>
+
-
</span>”<br><br>
+
-
 
+
-
<u>Buger mama:</u> <br>
+
-
”I’ve heard that citric acid can be produced by the mold, <i>Aspergillus niger</i>.”<br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Well, there you go! Can I have my lemonade then?”<br><br>
+
-
 
+
-
<u>Buger mama:</u> <br>
+
-
”Mmhmm.”<br><br>
+
-
 
+
-
<i>(Boy gets lemonade)</i><br><br>
+
-
 
+
-
<i>To 4</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">3.2</td>
+
-
    <td style="width:400px"> <b>No: </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<i>(*BOOM* to the classroom)</i><br><br>
+
-
 
+
-
<u>Professor:</u> <br>
+
-
”Did you know that many food products, for instance this lemonade you were just trying to
+
-
buy, contain additives that in cases are produced by genetically modified microorganisms?”<br><br>
+
-
<i>(Zooming in on lemonade declaration)</i><br><br>
+
-
”E330, citric acid, can be produced by the mold, <i>Aspergillus niger</i>, and E300, ascorbic acid,
+
-
or better known as vitamin C, can be produced by several bacteria, <i>Gluconobacter oxydans</i>
+
-
as an example. Different products might already be part of the microorganisms metabolism,
+
-
and the microorganisms have then been genetically engineered for an optimized metabolic
+
-
flux to be economically viable
+
-
<span class="sourceReference">producers</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
    Hancock, R.D.: Recent Patents on Vitamin C: Opportunities for Crop Improvement and Single-Step Biological Manufacture. Recent Patents on Food, Nutrition & Agriculture, 2009. Vol. 1, p. 39-49.
+
-
<a href="http://www.northsearegion.eu/files/repository/20131027214538_UK-Enclosures30.pdf" target="_blank">(Link)</a>
+
-
</span>
+
-
<span class="sourceReference"></span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
    Sauer, M., et al.: Microbial production of organic acids: expanding the market. Elsevier, 2008. Cell Press, vol. 26:2, p. 100-108.
+
-
<a href="http://www.northsearegion.eu/files/repository/20131027214538_UK-Enclosures30.pdf" target="_blank">(Link)</a>
+
-
</span>.<br><br>
+
-
It is advantageous to use genetically modified microorganisms for industrial production of
+
-
several additives, vitamins and flavors, because of their rapid growth, and in most cases, easy
+
-
cultivation. The microbial production is also more environmentally friendly than conventional
+
-
methods, since the microorganisms don’t need harsh chemicals, high temperatures or
+
-
pressures, and thus uses less
+
-
<span class="sourceReference">energy</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
  GMO Compass, 2006: GM Microorganisms Taking the Place of Chemical Factories.
+
-
<a href="http://www.gmo-compass.org/eng/grocery_shopping/ingredients_additives/36.gm_microorganisms_taking_place_chemical_factories.html" target="_blank">(Link)</a>
+
-
</span>.”<br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Oh!”<br><br>
+
-
 
+
-
<i>(*BOOM* to burger joint)</i><br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Well, burger mama. I got really thirsty from all this talk about bacteria, DNA and GMO. I
+
-
could really use that lemonade anyway.”<br><br>
+
-
 
+
-
<u>Burger mama:</u> <br>
+
-
”You got it!”<br><br>
+
-
 
+
-
<i>(Boy gets lemonade)</i><br><br>
+
-
 
+
-
<i>To 4</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">4.</td>
+
-
    <td style="width:400px"> <b>Boy and friend - iGEM </b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Boy:</u> <br>
+
-
”Thanks a lot for the service and talk, burger mama.”<br><br>
+
-
 
+
-
<u>Burger mama:</u> <br>
+
-
”Thank you. Enjoy your food and come again soon.”<br><br>
+
-
 
+
-
<i>(Boy waves at burger mama and friend arrives)</i><br><br>
+
-
 
+
-
<u>Friend:</u> <br>
+
-
”What’up, bro? What took you so long?”
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”I’ve had the craziest time ordering. Burger mama made me consider a lot of weird stuff
+
-
regarding my food.”<br><br>
+
-
 
+
-
<u>Friend:</u> <br>
+
-
”Like what?”<br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Whether I wanted it to contain bacteria, DNA and be produced by GMO. And then there was  
+
-
this hysterical man, running around afraid of GMO!”<br><br>
+
-
 
+
-
<u>Friend:</u> <br>
+
-
”Arh, GMO. Ha, that’s funny. You know, it makes me think of this friend of mine. He’s doing
+
-
some project at the moment – have you heard about iGEM before?”<br><br>
+
-
 
+
-
<i>(Boy looks confused into camera – CHOICE: 4.1 or 4.2)</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">4.1</td>
+
-
    <td style="width:400px"> <b>Yes:</b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Friend:</u> <br>
+
-
”Oh, cool. It sounds really exciting! You know, my friend I told you about, he’s part of the
+
-
SDU iGEM team 2014. They’re trying to make a bacteria that utilizes an otherwise non-
+
-
degradable source for humans, like cellulose, to produce a wonder protein, containing the  
+
-
required ratio of essential amino acids for humans, and to produce essential fatty acids, like
+
-
omega-3 and omega-6. It’s some of the optimal nutrition a human needs. At the same time,
+
-
they will make it taste good! It’s an Edible coli!”<br><br>
+
-
 
+
-
<u>Boy:</u> <br>
+
-
”Wow. That sounds truly amazing!”<br><br>
+
-
 
+
-
<u>Friend:</u> <br>
+
-
”If you like, we can go find the SDU iGEM team 2014?”<br><br>
+
-
 
+
-
<i>(Boy looks confused into camera – CHOICE: 5. or 6.)</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">4.2</td>
+
-
    <td style="width:400px"> <b>No:</b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Friend:</u> <br>
+
-
”iGEM stands for International Genetically Engineered Machine. It’s a worldwide
+
-
synthetic biology competition, founded by MIT, Boston. The competition initially aimed at
+
-
undergraduate university students, but has now expanded to include a high school division
+
-
and an entrepreneurship division. The goal of every team is to design and build their own
+
-
biological system and operate them in living cells – a
+
-
<span class="sourceReference">genetically engineered machine</span>
+
-
<span class="tooltip">
+
-
  <span class="tooltipHeader">Source:</span>
+
-
  Homepage of iGEM: Synthetic Biology – based on standard parts.
+
-
<a href="parts. http://www.igem.org/
+
-
Main_Page" target="_blank">(Link)</a>
+
-
</span>
+
-
.<br><br>
+
-
If you like to hear more, we can go find the SDU iGEM team 2014?”<br><br>
+
-
 
+
-
<i>(Boy looks confused into camera – CHOICE: 5. or 6.)</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">5.</td>
+
-
    <td style="width:400px"> <b>Skipping:</b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<u>Friend:</u> <br>
+
-
”Okay. Well, if you change your mind and want to hear more about iGEM and the SDU team
+
-
2014 another time, you can always find them here.”<br><br>
+
-
 
+
-
<i>(Link to <a href="https://www.igem.org/Main_Page">iGEM</a>, <a href="https://2014.igem.org/Team:SDU-Denmark">wiki</a>, <a href="https://www.facebook.com/iGEMSDUDenmark">Facebook</a>, <a https://twitter.com/iGEMSDU2014">Twitter</a>, <a href="http://instagram.com/igem.sdu.2014">Instagram</a>)</i> <br><br>
+
-
 
+
-
<i>The end</i><br><br>
+
-
</td>
+
-
</tr>
+
-
 
+
-
<tr>
+
-
    <td style="width:50px">6.</td>
+
-
    <td style="width:400px"> <b>Sure. Let's go:</b></td>
+
-
  </tr>
+
-
  <tr>
+
-
    <td style="width:50px"> </td>
+
-
    <td style="width:400px">
+
-
<i>(Fast forward from SDU main entrance to microbiology lab – presentation of the team)</i><br><br>
+
-
<i>The end</i>
+
-
</td>
+
-
</tr>
+
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<h4>Article in SUND&HED</h4>
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<span class="intro">SUND&HED is a university newspaper</span> directed to health science and medical students. We wrote an article
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about iGEM and our project, which will be published in late October.
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Latest revision as of 03:10, 18 October 2014

Edible coli
iGEM logo
Introduction
Edible coli
Process
Results
Policy and practices
Future

Sharing the message

"When you can't make them see the light, make them feel the heat." – Ronald Reagan

To present a scientific project is very educational. It requires a good understanding of the project and might lead to a better understanding of the details. Furthermore response and questions from listeners can be very helpful, as they give an idea of the understanding of the subject as well as show new aspects and reflections on the project.
We think that it is important to practice presentation skills and use all the benefits a presentation might lead to. Therefore we participated in different outreach events, where all team members have practiced their individual presentation skills at least once.
We spend much time on outreach, but we can conclude that the benefit, especially in the form of response, had a big impact on our project.


iGEM meet-up in London.

iGEM meet-up in London hosted by YSB (1st of September - 2nd of September)

We were very happy to participate in the iGEM meet-up in London. Here we got the first opportunity to tell others about our project and discuss our policies and practices with other iGEM teams. Additionally, there were a lot of friendly participants who helped by filling out our questionnaire and giving us valuable feedback.


Study Trade Fair (11th of September)

Study Trade Fair. We participated in the annual Study Trade Fair at our university to raise awareness about the iGEM competition, our project, promote our outreach events in October, to get more answers to our questionnaire and to promote the recruiting for next year’s iGEM-team. The day was a great success. We met many interested students who wanted to hear more about iGEM in general and about our project. In addition, we were able to practice our presentation skills.


Old-iGEM Meet-up (18th of September)

Old-GEM meet-up.

We invited all former SDU-iGEM participants to a meet up… and it was a success! They heard about our project and responded to our presentation with valuable feedback. Our supervisors had arranged and prepared dinner after our presentation, which gave us a chance to discuss the responses. Thank you to all of them for coming and making this a cozy evening.


Bioscientific dissemination (30th of September)

Bioscientific dissemination is an event for students by students. It is an event where students get the chance to present their individual, bachelor and master projects to get a great response and practice presentation skills. The iGEM SDU-Denmark 2014 project was presented by one of our team members, and he got constructive criticism as well as a great experience.


Sundhedsmekka: An exhibition for medical students (1st of October)

Sundhedsmekka. Sundhedsmekka is a yearly event, where associations and event groups with interests that concerns medical students can promote themselves. Originally, it was a medical student who started the first iGEM team from SDU. Since then the iGEM teams representing SDU have at least had one medical student on their team. In spite of this, most medical students at SDU have not heard of iGEM, and even synthetic biology is an unfamiliar field to most medical students. By participating in Sundhedsmekka our iGEM team got a chance to explain the concept of the iGEM contest and our own project. It was a great success to talk to all the many interested students and in connection with the conversations; we invited people to attend our “Quiz and Presentation night”, which had premiere in the following week.


Quiz and Presentation night at the Student House (6th of October)

Quiz and Presentation night at the Student House. The Student House is a gathering place for students and locals, which is located in the center of Odense. The purpose of the Student House is to have a forum where university students can come to study or to take part in social events. Volunteers are responsible for the café area, where different events take place frequently. Our team arranged a Microorganism Quiz and Presentation Night at the Student House where around fifty people participated. Our presentation was about the main concepts of iGEM and synthetic biology and additionally an overview of our own project. The presentation was followed by our quiz about microorganisms. Every question had a preamble concerning microorganisms but the question usually was a digression from the subject. Our idea with the event was that the questions should apply to anyone and it ended up being a great success for this reason.

Article in SUND&HED

SUND&HED is a university newspaper directed to health science and medical students. We wrote an article about iGEM and our project, which will be published in late October.


Next chapter

Next chapter

Dig deeper

End of tour

Want more? Go to iGEM.org!

If you want to learn more about Synthetic Biology you should visit iGEM's homepage, which contains loads on the subject. You can even visit other teams' wikis and take a look at what they accomplished. Or maybe join a team yourself?

A tour for everyone

Click here to begin the interactive wiki-tour

Take a quick glance at our project or dig deeper to get a complete walkthrough of the wiki. Or do something in-between, the choice is yours.

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