Team:SDU-Denmark/Tour35

From 2014.igem.org

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<h3> Enzymes </h3>
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<h3> 1.1.1.3 </h3>
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  <th>EC Number</th>
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  <th> Pathway</th>
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  <th>References</th>
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  <td><a href="https://2014.igem.org/Team:SDU-Denmark/Tour36">1.1.1.3</a></td>
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  <td>Methionine</td>
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  <td><a href="http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.1.1.3">BRENDA</a></td>
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  <td><a href="https://2014.igem.org/Team:SDU-Denmark/Tour36">1.1.1.85</a></td>
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  <td>Leucine</td>
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  <td><a href="http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.1.1.85">BRENDA</a></td>
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  <td><a href="https://2014.igem.org/Team:SDU-Denmark/Tour36">1.1.1.95</a></td>
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  <td>Serine</td>
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  <td><a href="http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.1.1.95">BRENDA</a></td>
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  <td><a href="https://2014.igem.org/Team:SDU-Denmark/Tour36">1.5.1.2</a></td>
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  <td>Proline</td>
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  <td><a href="http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.5.1.2">BRENDA</a></td>
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L-aspartate-4-semialdehyde + NAD(P)H+H<sup>+</sup> <font size="5">&#8652;</font> homoserine + NAD(P)<sup>+</sup> <br>
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<img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54" > <br>
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V<sub>max,f</sub>=2.8; From Glycine Max <br>
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V<sub>max,r</sub>=0.24; From Glycine Max <br>
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K<sub>m,A</sub>=0.17; From E.coli <br>
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K<sub>m,B</sub>=0.013; From E.coli
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<h3> 1.1.1.85 </h3>
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2-S-2-isopropylmalate + NAD<sup>+</sup> <font size="5">&#8594;</font> 4-methyl-2-oxopentanoate + NADH + H<sup>+</sup> <br>
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<img src="https://static.igem.org/mediawiki/2014/7/7b/SDU20141_1_1_85_example.PNG" width="123" height="47" > <br>
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V<sub>max</sub>=69; From E.coli <br>
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K<sub>m</sub>=0.321; From E.coli <br>
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</p>
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Back to Enzymes page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour35">Enzymes</a> <br>
Back to modelling page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour32">Modelling</a>
Back to modelling page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour32">Modelling</a>

Revision as of 17:57, 9 September 2014

Edible coli
iGEM logo
Introduction
Edible coli
Process
Results
Policy and practices
Future

1.1.1.3

L-aspartate-4-semialdehyde + NAD(P)H+H+ ⇌ homoserine + NAD(P)+

Vmax,f=2.8; From Glycine Max
Vmax,r=0.24; From Glycine Max
Km,A=0.17; From E.coli
Km,B=0.013; From E.coli

1.1.1.85

2-S-2-isopropylmalate + NAD+ → 4-methyl-2-oxopentanoate + NADH + H+

Vmax=69; From E.coli
Km=0.321; From E.coli

Back to Enzymes page: Enzymes
Back to modelling page: Modelling

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