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Team:SDU-Denmark/Tour35
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We considered the rate function as if it was on the form: <b> A <font size="5">⇌</font> B </b>. We do not look at NAD(P)H+H<sup>+</sup> and NAD(P)<sup>+</sup>. <br> | We considered the rate function as if it was on the form: <b> A <font size="5">⇌</font> B </b>. We do not look at NAD(P)H+H<sup>+</sup> and NAD(P)<sup>+</sup>. <br> | ||
<b> Rate function: </b> <br> | <b> Rate function: </b> <br> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54" > (click picture for copyable formula with constants inserted.)<br> | + | <a href="https://2014.igem.org/Team:SDU-Denmark/Tour36"><img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54" > (click picture for copyable formula with constants inserted.)<br> |
V<sub>max,f</sub>=2.8; From Glycine Max <br> | V<sub>max,f</sub>=2.8; From Glycine Max <br> | ||
V<sub>max,r</sub>=0.24; From Glycine Max <br> | V<sub>max,r</sub>=0.24; From Glycine Max <br> | ||
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We considered the rate function as if it was on the form: <b> A <font size="5">→</font> B </b>. We do not look at NAD<sup>+</sup>, NADH and H<sup>+</sup>. <br> | We considered the rate function as if it was on the form: <b> A <font size="5">→</font> B </b>. We do not look at NAD<sup>+</sup>, NADH and H<sup>+</sup>. <br> | ||
<b> Rate function: </b> <br> | <b> Rate function: </b> <br> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/7/7b/SDU20141_1_1_85_example.PNG" width="123" height="47" > (click picture for copyable formula with constants inserted.)<br> | + | <a href="https://2014.igem.org/Team:SDU-Denmark/Tour37"><img src="https://static.igem.org/mediawiki/2014/7/7b/SDU20141_1_1_85_example.PNG" width="123" height="47" > (click picture for copyable formula with constants inserted.)<br> |
V<sub>max</sub>=69; From E.coli <br> | V<sub>max</sub>=69; From E.coli <br> | ||
K<sub>m</sub>=0.321; From E.coli <br> | K<sub>m</sub>=0.321; From E.coli <br> | ||
Revision as of 18:33, 9 September 2014
1.1.1.3 - homoserine dehydrogenase
L-aspartate-4-semialdehyde + NAD(P)H+H+ ⇌ homoserine + NAD(P)+
Model submitted by SDU-Denmark 2014:
Notes:
We considered the rate function as if it was on the form: A ⇌ B . We do not look at NAD(P)H+H+ and NAD(P)+.
Rate function:
(click picture for copyable formula with constants inserted.)Vmax,f=2.8; From Glycine Max
Vmax,r=0.24; From Glycine Max
Km,A=0.17; From E.coli
Km,B=0.013; From E.coli
1.1.1.85 - 3-isopropylmalate dehydrogenase
2-S-2-isopropylmalate + NAD+ → 4-methyl-2-oxopentanoate + NADH + H+
Model submitted by SDU-Denmark 2014:
Notes:
We considered the rate function as if it was on the form: A → B . We do not look at NAD+, NADH and H+.
Rate function:
(click picture for copyable formula with constants inserted.)Vmax=69; From E.coli
Km=0.321; From E.coli
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