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Team:SDU-Denmark/Tour35
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<h3> 1.1.1.3 </h3> | <h3> 1.1.1.3 </h3> | ||
| + | L-aspartate-4-semialdehyde + NAD(P)H+H<sup>+</sup> <font size="5">⇌</font> homoserine + NAD(P)<sup>+</sup> | ||
<h5> Submitted by SDU-Denmark 2014: </h5> <br> | <h5> Submitted by SDU-Denmark 2014: </h5> <br> | ||
<b> Notes: </b> <br> | <b> Notes: </b> <br> | ||
We considered the rate function as if it was on the form: <b> A <font size="5">⇌</font> B </b>. We do not look at NAD(P)H+H<sup>+</sup> and NAD(P)<sup>+</sup>. <br> | We considered the rate function as if it was on the form: <b> A <font size="5">⇌</font> B </b>. We do not look at NAD(P)H+H<sup>+</sup> and NAD(P)<sup>+</sup>. <br> | ||
<b> Rate function: </b> <br> | <b> Rate function: </b> <br> | ||
| - | |||
<img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54" > <br> | <img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54" > <br> | ||
V<sub>max,f</sub>=2.8; From Glycine Max <br> | V<sub>max,f</sub>=2.8; From Glycine Max <br> | ||
Revision as of 18:21, 9 September 2014
1.1.1.3
L-aspartate-4-semialdehyde + NAD(P)H+H+ ⇌ homoserine + NAD(P)+Submitted by SDU-Denmark 2014:
Notes:
We considered the rate function as if it was on the form: A ⇌ B . We do not look at NAD(P)H+H+ and NAD(P)+.
Rate function:
Vmax,f=2.8; From Glycine Max
Vmax,r=0.24; From Glycine Max
Km,A=0.17; From E.coli
Km,B=0.013; From E.coli
1.1.1.85
2-S-2-isopropylmalate + NAD+ → 4-methyl-2-oxopentanoate + NADH + H+
Vmax=69; From E.coli
Km=0.321; From E.coli
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