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Team:SDU-Denmark/Tour27
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| - | <h3> 1.1.1.3 - homoserine dehydrogenase</h3> | + | <h3>1.1.1.3 - homoserine dehydrogenase</h3> |
<h4>L-aspartate-4-semialdehyde + NAD(P)H+H<sup>+</sup> <font size="5">⇌</font> homoserine + NAD(P)<sup>+</sup> </h4><br> | <h4>L-aspartate-4-semialdehyde + NAD(P)H+H<sup>+</sup> <font size="5">⇌</font> homoserine + NAD(P)<sup>+</sup> </h4><br> | ||
| - | <h5> Model submitted by SDU-Denmark 2014 | + | <h5>Model submitted by SDU-Denmark 2014 </h5> <br> |
| - | < | + | <span class = intro>Notes/Assumption: </span> <br> |
We do not include NAD(P)H+H<sup>+</sup> and NAD(P)<sup>+</sup> in the model. <br> <br> | We do not include NAD(P)H+H<sup>+</sup> and NAD(P)<sup>+</sup> in the model. <br> <br> | ||
| - | < | + | <span class = intro>The reaction is on the form: </span> A <font size="5">⇌</font> B <br> |
<b> A: </b> L-aspartate-4-semialdehyde <br> | <b> A: </b> L-aspartate-4-semialdehyde <br> | ||
<b> B: </b> Homoserine <br> <br> | <b> B: </b> Homoserine <br> <br> | ||
| - | < | + | <span class = intro>Rate function:</span> <br><br> |
| - | <a href="https://2014.igem.org/Team:SDU-Denmark/Tour28"><img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54"></a> (click picture for copyable formula with constants inserted | + | <a href="https://2014.igem.org/Team:SDU-Denmark/Tour28"><img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54"></a> (<b>click picture</b> for copyable formula with constants inserted)<br><br> |
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| + | <th class="tg-e3zv">Constant</th> | ||
| + | <th class="tg-e3zv">Value</th> | ||
| + | <th class="tg-e3zv">Comment</th> | ||
| + | <th class="tg-e3zv">Reference</th> | ||
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| + | <td class="tg-031e">V<sub>max,forward</sub></td> | ||
| + | <td class="tg-031e">2.8</td> | ||
| + | <td class="tg-031e">Value obtained from Glycine Max</td> | ||
| + | <td class="tg-031e">Brenda</td> | ||
| + | </tr> | ||
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| + | <td class="tg-031e">V<sub>max,reverse</sub></td> | ||
| + | <td class="tg-031e">0.24</td> | ||
| + | <td class="tg-031e">Value obtained from Glycine Max</td> | ||
| + | <td class="tg-031e">Brenda</td> | ||
| + | </tr> | ||
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| + | <td class="tg-031e">K<sub>m,A</sub></td> | ||
| + | <td class="tg-031e">0.17</td> | ||
| + | <td class="tg-031e">Value obtained from E. coli</td> | ||
| + | <td class="tg-031e">Brenda</td> | ||
| + | </tr> | ||
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| + | <td class="tg-031e">K<sub>m,B</sub></td> | ||
| + | <td class="tg-031e">0.013</td> | ||
| + | <td class="tg-gmhj">Value obtained from E. coli</td> | ||
| + | <td class="tg-gmhj">Brenda</td> | ||
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Latest revision as of 23:16, 17 October 2014
1.1.1.3 - homoserine dehydrogenase
L-aspartate-4-semialdehyde + NAD(P)H+H+ ⇌ homoserine + NAD(P)+
Model submitted by SDU-Denmark 2014
Notes/Assumption:
We do not include NAD(P)H+H+ and NAD(P)+ in the model.
The reaction is on the form: A ⇌ B
A: L-aspartate-4-semialdehyde
B: Homoserine
Rate function:
(click picture for copyable formula with constants inserted)| Constant | Value | Comment | Reference |
|---|---|---|---|
| Vmax,forward | 2.8 | Value obtained from Glycine Max | Brenda |
| Vmax,reverse | 0.24 | Value obtained from Glycine Max | Brenda |
| Km,A | 0.17 | Value obtained from E. coli | Brenda |
| Km,B | 0.013 | Value obtained from E. coli | Brenda |
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