Team:SDU-Denmark/Tour27

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Latest revision as of 23:16, 17 October 2014

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1.1.1.3 - homoserine dehydrogenase

L-aspartate-4-semialdehyde + NAD(P)H+H⁺ ⇌ homoserine + NAD(P)⁺

Model submitted by SDU-Denmark 2014

Notes/Assumption:
We do not include NAD(P)H+H⁺ and NAD(P)⁺ in the model.

The reaction is on the form: A ⇌ B
A: L-aspartate-4-semialdehyde
B: Homoserine

Rate function:

(click picture for copyable formula with constants inserted)

Constant	Value	Comment	Reference
V_max,forward	2.8	Value obtained from Glycine Max	Brenda
V_max,reverse	0.24	Value obtained from Glycine Max	Brenda
K_m,A	0.17	Value obtained from E. coli	Brenda
K_m,B	0.013	Value obtained from E. coli	Brenda

Back to Enzymes page: Enzymes
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-<h3> 1.1.1.3 - homoserine dehydrogenase</h3>
+<h3>1.1.1.3 - homoserine dehydrogenase</h3>
 <h4>L-aspartate-4-semialdehyde + NAD(P)H+H<sup>+</sup> <font size="5">&#8652;</font> homoserine + NAD(P)<sup>+</sup> </h4><br>
-<h5> Model submitted by SDU-Denmark 2014: </h5> <br>
+<h5>Model submitted by SDU-Denmark 2014 </h5> <br>
-<b> Notes: </b> <br>
+<span class = intro>Notes/Assumption: </span> <br>
-We considered the rate function as if it was on the form: <b> A <font size="5">&#8652;</font> B </b>. We do not look at NAD(P)H+H<sup>+</sup> and NAD(P)<sup>+</sup>. <br>
+We do not include NAD(P)H+H<sup>+</sup> and NAD(P)<sup>+</sup> in the model. <br> <br>
-<b> Rate function: </b> <br>
+<span class = intro>The reaction is on the form: </span> A <font size="5">&#8652;</font> B <br>
-<a href="https://2014.igem.org/Team:SDU-Denmark/Tour37"><img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54"></a> (click picture for copyable formula with constants inserted.)<br>
+<b> A: </b> L-aspartate-4-semialdehyde <br>
-V<sub>max,f</sub>=2.8; From Glycine Max <br>
+<b> B: </b> Homoserine <br> <br>
-V<sub>max,r</sub>=0.24; From Glycine Max <br>
+<span class = intro>Rate function:</span>  <br><br>
-K<sub>m,A</sub>=0.17; From E.coli <br>
+<a href="https://2014.igem.org/Team:SDU-Denmark/Tour28"><img src="https://static.igem.org/mediawiki/2014/3/3c/SDU20141_1_1_3_example.PNG" width="265" height="54"></a>  &nbsp; &nbsp;(<b>click picture</b> for copyable formula with constants inserted)<br><br>
-K<sub>m,B</sub>=0.013; From E.coli <br>
-<br>
 <br>
-Back to Enzymes page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour35">Enzymes</a> <br>
+<style type="text/css">
-Back to modelling page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour34">Modelling</a>
+.tg  {border-collapse:collapse;border-spacing:0;border-color:#ccc;}
+.tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-color:#ccc;color:#333;background-color:#fff;border-top-width:1px;border-bottom-width:1px;}
+.tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-color:#ccc;color:#333;background-color:#f0f0f0;border-top-width:1px;border-bottom-width:1px;}
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+<col style="width: 86px">
+<col style="width: 61px">
+<col style="width: 236px">
+<col style="width: 91px">
+</colgroup>
+  <tr>
+    <th class="tg-e3zv">Constant</th>
+    <th class="tg-e3zv">Value</th>
+    <th class="tg-e3zv">Comment</th>
+    <th class="tg-e3zv">Reference</th>
+  </tr>
+  <tr>
+    <td class="tg-031e">V<sub>max,forward</sub></td>
+    <td class="tg-031e">2.8</td>
+    <td class="tg-031e">Value obtained from Glycine Max</td>
+    <td class="tg-031e">Brenda</td>
+  </tr>
+  <tr>
+    <td class="tg-031e">V<sub>max,reverse</sub></td>
+    <td class="tg-031e">0.24</td>
+    <td class="tg-031e">Value obtained from Glycine Max</td>
+    <td class="tg-031e">Brenda</td>
+  </tr>
+  <tr>
+    <td class="tg-031e">K<sub>m,A</sub></td>
+    <td class="tg-031e">0.17</td>
+    <td class="tg-031e">Value obtained from E. coli</td>
+    <td class="tg-031e">Brenda</td>
+  </tr>
+  <tr>
+    <td class="tg-031e">K<sub>m,B</sub></td>
+    <td class="tg-031e">0.013</td>
+    <td class="tg-gmhj">Value obtained from E. coli</td>
+    <td class="tg-gmhj">Brenda</td>
+  </tr>
+</table>
+<br> <br>
+<span class = intro>Back to Enzymes page:</span> <a href="https://2014.igem.org/Team:SDU-Denmark/Tour25">Enzymes</a> <br>
+<span class = intro>Back to modelling page:</span> <a href="https://2014.igem.org/Team:SDU-Denmark/Tour24">Modelling</a>
+<br><br><br>
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