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Team:SDU-Denmark/Tour23
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| - | <p> | + | <h3>System design</h3> |
| - | + | <p class='intro'> | |
| - | + | <font color="3397FE">Think big – safe the world!</font> | |
| - | + | ||
</p> | </p> | ||
| + | <h4>Original thought</h4> | ||
| + | The original design of the system making up the Edible coli, is a K12 MG1655 strain of <i>Escherichia coli</i>: | ||
| + | <ol> | ||
| + | <li> <u>Excreting cellulases for the degradation of cellulose to glucose:</u><br> | ||
| + | Cellulose (C<sub>6</sub>H<sub>10</sub>O<sub>5</sub>)<sub>n</sub> consists of β-1,4 linked D-glucose units. For the Edible coli to gain nutrients | ||
| + | from cellulose in the form of glucose units, the β-glucosidic bonds in-between must be broken by | ||
| + | hydrolysis. For this degradation, three enzymatic activities are needed by the enzymes, collectively | ||
| + | known as cellulases: Endoglucanase, exoglucanase and β-glucosidase. Endoglucanase hydrolyses | ||
| + | internal β-1,4 glucosidic bonds in the cellulose fiber, while exoglucanase hydrolyses the external | ||
| + | bonds, releasing cellobiose disaccharides. The cellobiose disaccharides are then cleaved by β- | ||
| + | glucosidase into two glucose molecules | ||
| + | <span class="sourceReference">each.</span> | ||
| + | <span class="tooltip"> | ||
| + | <span class="tooltipHeader">Source:</span> | ||
| + | Lynd, L.R., et al.: Microbial Cellulose Utilization: Fundamentals and Biotechnology. Microbiology and Molecular Biology Reviews, 2002. Vol. 66:3, p. 506-577. | ||
| + | <a href="http://mmbr.asm.org/content/66/3/506.long" target="_blank">(Link)</a></span> | ||
| + | </li> | ||
</html> | </html> | ||
{{:Team:SDU-Denmark/core/footer}} | {{:Team:SDU-Denmark/core/footer}} | ||
Revision as of 22:02, 14 October 2014
System design
Think big – safe the world!
Original thought
The original design of the system making up the Edible coli, is a K12 MG1655 strain of Escherichia coli:- Excreting cellulases for the degradation of cellulose to glucose:
Cellulose (C6H10O5)n consists of β-1,4 linked D-glucose units. For the Edible coli to gain nutrients from cellulose in the form of glucose units, the β-glucosidic bonds in-between must be broken by hydrolysis. For this degradation, three enzymatic activities are needed by the enzymes, collectively known as cellulases: Endoglucanase, exoglucanase and β-glucosidase. Endoglucanase hydrolyses internal β-1,4 glucosidic bonds in the cellulose fiber, while exoglucanase hydrolyses the external bonds, releasing cellobiose disaccharides. The cellobiose disaccharides are then cleaved by β- glucosidase into two glucose molecules each. Source: Lynd, L.R., et al.: Microbial Cellulose Utilization: Fundamentals and Biotechnology. Microbiology and Molecular Biology Reviews, 2002. Vol. 66:3, p. 506-577. (Link)
