Team:SDU-Denmark/Tour35

From 2014.igem.org

(Difference between revisions)
Line 15: Line 15:
K<sub>m,B</sub>=0.013; From E.coli
K<sub>m,B</sub>=0.013; From E.coli
-
<h3> 1.1.1.85 </h3>
+
<h3> 1.1.1.85 - 3-isopropylmalate dehydrogenase</h3>
-
 
+
<h4>2-S-2-isopropylmalate + NAD<sup>+</sup> <font size="5">&#8594;</font> 4-methyl-2-oxopentanoate + NADH + H<sup>+</sup></h4><br>
-
<p>
+
<h5> Model submitted by SDU-Denmark 2014: </h5> <br>
-
 
+
<b> Notes: </b> <br>
-
2-S-2-isopropylmalate + NAD<sup>+</sup> <font size="5">&#8594;</font> 4-methyl-2-oxopentanoate + NADH + H<sup>+</sup> <br>
+
fjdlafjd. <br>
<img src="https://static.igem.org/mediawiki/2014/7/7b/SDU20141_1_1_85_example.PNG" width="123" height="47" > <br>
<img src="https://static.igem.org/mediawiki/2014/7/7b/SDU20141_1_1_85_example.PNG" width="123" height="47" > <br>
V<sub>max</sub>=69; From E.coli <br>
V<sub>max</sub>=69; From E.coli <br>
K<sub>m</sub>=0.321; From E.coli <br>
K<sub>m</sub>=0.321; From E.coli <br>
 +
<br>
-
</p>
 
Back to Enzymes page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour34">Enzymes</a> <br>
Back to Enzymes page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour34">Enzymes</a> <br>
Back to modelling page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour33">Modelling</a>
Back to modelling page: <a href="https://2014.igem.org/Team:SDU-Denmark/Tour33">Modelling</a>

Revision as of 18:26, 9 September 2014

1.1.1.3 - homoserine dehydrogenase

L-aspartate-4-semialdehyde + NAD(P)H+H+ homoserine + NAD(P)+


Model submitted by SDU-Denmark 2014:

Notes:
We considered the rate function as if it was on the form: A B . We do not look at NAD(P)H+H+ and NAD(P)+.
Rate function:

Vmax,f=2.8; From Glycine Max
Vmax,r=0.24; From Glycine Max
Km,A=0.17; From E.coli
Km,B=0.013; From E.coli

1.1.1.85 - 3-isopropylmalate dehydrogenase

2-S-2-isopropylmalate + NAD+ 4-methyl-2-oxopentanoate + NADH + H+


Model submitted by SDU-Denmark 2014:

Notes:
fjdlafjd.

Vmax=69; From E.coli
Km=0.321; From E.coli

Back to Enzymes page: Enzymes
Back to modelling page: Modelling