Team:Heidelberg/AwesomeSheet/Samples

From 2014.igem.org

– Samples

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O
1		Name	Experimantor	Date taken	Time taken	original OD600	Related Growth	Related Induction	Description	POI		POI 2		POI 3
2										Name	MW	Name	MW	Name	MW
3											kDa		kDa		kDa

4	27	6u	Jan	06-08	14:36	1.08	356c1-2	23	Uninduced
5	28	6:1 1/2	Jan	06-08	17:52	1.96	356c1-2	23	1.5 h after induction with 0.4 mM IPTG	H6-GB1-NpuDnaEN	20.4	H6-GB1	8.5	NpuDnaEN	11.9
6	29	6:3h	Jan	06-08	19:23	2.7	356c1-2	23	3 h after induction with 0.4 mM IPTG	H6-GB1-NpuDnaEN	20.4	H6-GB1	8.5	NpuDnaEN	11.9
7	30	6:ON	Jan	06-08	10:00	11	356c1-2	23	16 h after induction with 0.4 mM IPTG	H6-GB1-NpuDnaEN	20.4	H6-GB1	8.5	NpuDnaEN	11.9
8	31	7u	Jan	06-08	14:36	0.96	357c1-2	23	Uninduced
9	32	7:1 1/2	Jan	06-08	17:30	1.6	357c1-2	23	1.5 h after induction with 0.4 mM IPTG	H6-NpuDnaEC-IgG	15.5	H6-NpuDnaEC	6.3	IgG	9.3
10	33	7:3h	Jan	06-08	18:55	2.4	357c1-2	23	3 h after induction with 0.4 mM IPTG	H6-NpuDnaEC-IgG	15.5	H6-NpuDnaEC	6.3	IgG	9.3
11	34	7:ON	Jan	06-08	10:00	10.4	357c1-2	23	16 h after induction with 0.4 mM IPTG	H6-NpuDnaEC-IgG	15.5	H6-NpuDnaEC	6.3	IgG	9.3
12	35	8u	Jan	06-08	14:36	1.03	358c1-2	23	Uninduced
13	36	8:1 1/2	Jan	06-08	19:23	2.1	358c1-2	23	1.5 h after induction with 0.4 mM IPTG	Strep-NpuDnaEC-eGFP-H6
14	37	8:3h	Jan	06-08	20:50	3.27	358c1-2	23	3 h after induction with 0.4 mM IPTG
15	38	8:ON	Jan	06-08	10:00	2.15	358c1-2	23	16 h after induction with 0.4 mM IPTG
16	39	9u	Jan	06-08	14:36	0.96	359c1-2	23	Uninduced
17	40	9:1 1/2	Jan	06-08	17:52	1.88	359c1-2	23	1.5 h after induction with 0.4 mM IPTG
18	41	9:3h	Jan	06-08	19:23	2.79	359c1-2	23	3 h after induction with 0.4 mM IPTG
19	42	9:ON	Jan	06-08	10:00	4.7	359c1-2	23	16 h after induction with 0.4 mM IPTG
20	43	10u	Jan	06-08	14:36	1.61	360c1-2	23	Uninduced
21	44	10:1 1/2	Jan	06-08	17:52	2.44	360c1-2	23	1.5 h after induction with 0.4 mM IPTG
22	45	10:3h	Jan	06-08	19:23	3.42	360c1-2	23	3 h after induction with 0.4 mM IPTG
23	46	10:ON	Jan	06-08	10:00	6	360c1-2	23	16 h after induction with 0.4 mM IPTG
24	47	11u	Jan	06-08	14:36	1.32	361c1-2	23	Uninduced
25	48	11:1 1/2	Jan	06-08	17:30	2.3	361c1-2	23	1.5 h after induction with 0.4 mM IPTG
26	49	11:3h	Jan	06-08	18:55	3.15	361c1-2	23	3 h after induction with 0.4 mM IPTG
27	50	11:ON	Jan	06-08	10:00	6	361c1-2	23	16 h after induction with 0.4 mM IPTG
28	51	pJJDuet30, pSFBAD09				4.4	353C1	19
29	52	pJJDuet30				12.4	354c1	21
30	53	pSFBAD09				3.2	355c1	21
31	54	pSB1AK8-BBa_I712074-2ZSspH6:GFP				23.4	372c1	25
32	55	pSB1AK8-BBa_I712074-2ZSspH6:GFP				30.4	352c1	18
33	56	pSBX4C5-2ZSspH6:GFP				18	299c1
34	57					7.4	300c1
35	58					10.2	351c1	18
36	59	GFP-His control				16.6
37	60	Inc GFP #2				10.4
38	61	Silvan 66				28.8
39	62	AK Npu Silvan					263C1-3	28	37°C	GFP	27.7
40	63	pSB1AK8-BBa_I712074-2ZSspH6:GFP					372C2	28	37°C	GFP	27.7
41	64	pSB1AK8-BBa_I712074-2ZSspH6:GFP					372C3	28	28°C	GFP	27.7
42	65	pSBX4C5-2ZSspH6:GFP					300C2	28	37°C	GFP	27.7
43	66	pSBX4C5-2ZSspH6:GFP					300C3	28	28°C	GFP	27.7
44	67	C+	Jan	08-27			536C1-1	39	3ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 50% power on ice, centrifuged at 18000 rcf for 10 min, supernatant	H6-NpuDnaE^C-sfGFP^C-H6	25.7	H6-NpuDnaE^C	20.7	sfGFP^C-H6	5
45	68	C*+	Jan	08-27			537C1-1	39	as sample 67	H6-NpuDnaE^C SN->AG-sfGFP^C-H6	25.7	H6-NpuDnaE^C*	20.7	sfGFP^C-H6	5
46	69	N+	Jan	08-27			538C1-1	39	as sample 67	H6-sfGFP^N-NpuDnaE^N-H6	20.4	NpuDnaE^N-H6	12.7	H6-sfGFP^N	7.7
47	70	N*+	Jan	08-27			539C1-1	39	as sample 67	H6-sfGFP^N-C->G NpuDnaE^N-H6	20.4	NpuDnaE^N*-H6	12.7	H6-sfGFP^N*	7.7
48	71	sfGFP+	Jan	08-27			540C1-1	39	as sample 67, FCS: ~1µM	H6-sfGFP-H6	28.5
49	72	sfGFP+	Jan	08-27			540C2-1	39	as sample 67	H6-sfGFP-H6	28.5
50	73	C+ Pellet	Jan	08-27			536C1-1	39	3ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 50% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS	H6-NpuDnaE^C-sfGFP^C-H6	25.7	H6-NpuDnaE^C	20.7	sfGFP^C-H6	5
51	74	C*+ Pellet	Jan	08-27			537C1-1	39	as sample 73	H6-NpuDnaE^C SN->AG-sfGFP^C-H6	25.7	H6-NpuDnaE^C*	20.7	sfGFP^C-H6	5
52	75	N+ Pellet	Jan	08-27			538C1-1	39	as sample 73	H6-sfGFP^N-NpuDnaE^N-H6	20.4	NpuDnaE^N-H6	12.7	H6-sfGFP^N	7.7
53	76	N*+ Pellet	Jan	08-27			539C1-1	39	as sample 73	H6-sfGFP^N-C->G NpuDnaE^N-H6	20.4	NpuDnaE^N*-H6	12.7	H6-sfGFP^N*	7.7
54	77	sfGFP+ Pellet	Jan	08-27			540C1-1	39	as sample 73	H6-sfGFP-H6	28.5
55	78	sfGFP+ Pellet	Jan	08-27			540C2-1	39	as sample 73	H6-sfGFP-H6	28.5
56	79	C-	Jan	08-27			536C1		5 ml, as sample 67	H6-NpuDnaE^C-sfGFP^C-H6	25.7	H6-NpuDnaE^C	20.7	sfGFP^C-H6	5
57	80	C*-	Jan	08-27			537C1		5 ml, as sample 67	H6-NpuDnaE^C SN->AG-sfGFP^C-H6	25.7	H6-NpuDnaE^C*	20.7	sfGFP^C-H6	5
58	81	N-	Jan	08-27			538C1		5 ml, as sample 67	H6-sfGFP^N-NpuDnaE^N-H6	20.4	NpuDnaE^N-H6	12.7	H6-sfGFP^N	7.7
59	82	N*-	Jan	08-27			539C1		5 ml, as sample 67	H6-sfGFP^N-C->G NpuDnaE^N-H6	20.4	NpuDnaE^N*-H6	12.7	H6-sfGFP^N*	7.7
60	83	sfGFP-	Jan	08-27			540C1		5 ml, as sample 67	H6-sfGFP-H6	28.5
61	84	sfGFP-	Jan	08-27			540C2		5 ml, as sample 67	H6-sfGFP-H6	28.5
62	85	C- Pellet	Jan	08-27			536C1		5 ml, as sample 67	H6-NpuDnaE^C-sfGFP^C-H6	25.7	H6-NpuDnaE^C	20.7	sfGFP^C-H6	5
63	86	C*- Pellet	Jan	08-27			537C1		5 ml, as sample73	H6-NpuDnaE^C SN->AG-sfGFP^C-H6	25.7	H6-NpuDnaE^C*	20.7	sfGFP^C-H6	5
64	87	N- Pellet	Jan	08-27			538C1		5 ml, as sample73	H6-sfGFP^N-NpuDnaE^N-H6	20.4	NpuDnaE^N-H6	12.7	H6-sfGFP^N	7.7
65	88	N*- Pellet	Jan	08-27			539C1		5 ml, as sample73	H6-sfGFP^N-C->G NpuDnaE^N-H6	20.4	NpuDnaE^N*-H6	12.7	H6-sfGFP^N*	7.7
66	89	sfGFP- Pellet	Jan	08-27			540C1		5 ml, as sample73	H6-sfGFP-H6	28.5
67	90	sfGFP- Pellet	Jan	08-27			540C2		5 ml, as sample73	H6-sfGFP-H6	28.5
68	91	C_SN	Caro	08-30			536C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
69	92	C*_SN	Caro	08-30			537C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
70	93	N_SN	Caro	08-30			538C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
71	94	N*_SN	Caro	08-30			539C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
72	95	CN_invivo__SN	Caro	08-30			552C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
73	96	sfGFP_SN	Caro	08-30			540C2-4		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
74	97	C_Pellet	Caro	08-30			536C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
75	98	C*_Pellet	Caro	08-30			537C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
76	99	N_Pellet	Caro	08-30			538C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
77	100	N*_Pellet	Caro	08-30			539C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
78	101	CN_invivo__Pellet	Caro	08-30			552C2-2		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
79	102	sfGFP_Pellet	Caro	08-30			540C2-4		20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
80	103	CN	Jan	08-29			552C3	40	as sample 67	H6-sfGFP-H6	28.5	H6-NpuDnaE^C	20.77	NpuDnaE^N-H6	12.7
81	104	CN Pellet	Jan	08-29			552C3	40	as sample 73	H6-sfGFP-H6	28.5	H6-NpuDnaE^C	20.77	NpuDnaE^N-H6	12.7
82	105	C+N	Jan	08-28					Mixed Sample 67 and 69
83	106	BI21DE3	Charlotte	8/26/2014		2	6C1-9		BI21DE3: background control
84	107	pSB1AK8-BBa_I712074-2ZSspH6:GFP	Charlotte	8/24/2014			510C2
85	108	pSB1C3-BBa_I746907	Charlotte	09-02			591C2		GFP control
86	109	pSB1AK8-T7-2ZNpu:(GFP-H6)	Charlotte	09-02			263C1-7
87	110	pSB1AK8-BBa_I712074-2ZSspH6:GFP	Charlotte	09-02			510C2-2
88	111	BI21(DE3)	Charlotte	09-02			6C1-10
89	112	pSB1C3-BBa_I746907	Charlotte	09-02			591C2		Pellet
90	113	pSB1AK8-T7-2ZNpu:(GFP-H6)	Charlotte	09-02			263C1-7		Pellet	GFP-H6_circ
91	114	pSB1AK8-BBa_I712074-2ZSspH6:GFP	Charlotte	09-02			510C2-2		Pellet
92	115	BI21(DE3)	Charlotte	09-02			6C1-10		Pellet
93	116	C+N SN	Caro	30-8					Mixed Sample 91 and 93
94	117	C+N SN	Caro	30-8					Mixed Sample 92 and 94
95	118	C+N Pellet	Caro	30-8					Mixed Sample 97 and 99
96	119	C+N Pellet	Caro	30-8					Mixed Sample 98 and 100
97	120	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	Jan	09-18	5:39	2.64	597C1-8	54	took 2ODml from Liquid culture, spun down at 18000rcf for 5 min removed supernatant stored at -55°C
98	121	pSBX1K3-LiLaLy(H6)	Charlotte	09-19			733C2		took 2ODml from Liquid culture, spun down at 18000rcf for 5 min removed supernatant stored at -55°C
99	122	Bl21(DE3)	Charlotte	09-22			6C1-14		background control
100	158	Lin. Xylanase	Charlotte	09-24			840C1-3
101	159	Circ. Xylanase	Charlotte	09-24			611C1-2
102	160	Lin. Xylanase + His	Charlotte	09-24			841C1-3
103	161	Circ. Xylanas + His	Charlotte	09-24			563C1-2
104	162	Lin. Xylanase + His + Ext.	Charlotte	09-24			923C1
105	163	Circ (destr. Ext.) Xylanase + His	Charlotte	09-24			598C1-2
106	164	BL21-6	Charlotte	09-24			6C1-16
107	165	Circ Xylanase. His (2)	Charlotte	09-24			611C1-2
108	166	Lin Xylanase (2)	Charlotte	09-24			841C1-3
109	167	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	Charlotte	09-25			436C1-11
110	168	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	Charlotte	09-25			596C1-7
111	169	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	Charlotte	09-25			597C1-9
112	170	pSBX1K3-LiLaLy(H6)	Charlotte	09-25			733C2-2
113	Dnmt1 #5	pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6, pRARE	Silvan	09-30			828C1-3		Dnmt1_Sort w/o linker
114	Dnmt1 #6	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT), pRARE	Silvan	09-30			978C1-1/2		Dnmt1_Intein_rigid linker
115	Dnmt1 #7	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT), pRARE	Silvan	09-30			1001C1-1/2		Dnmt1_Intein_flex linker
116	Dnmt1 #8		Silvan	09-30			1002C1-1/2		Dnmt1_Sort late rigid linker
117	175	360 - BL21 ctrl	Jakob	09-30
118	176	360 - N	Jakob	09-30
119	177	360 - C	Jakob	09-30
120	178	360 - N*	Jakob	09-30
121	179	360 - C*	Jakob	09-30
122	180	360 - NC	Jakob	09-30
123	181	360 - NC	Jakob	09-30
124	182	360 - sfGFP*	Jakob	09-30
125	183	360 - sfGFP	Jakob	09-30
126	184	000 - NC	Jakob	09-30
127	185	000 - NC	Jakob	09-30
128	186	030 - NC	Jakob	09-30
129	187	030 - NC	Jakob	09-30
130	188	060 - NC	Jakob	09-30
131	189	060 - NC	Jakob	09-30
132	190	120 - NC	Jakob	09-30
133	191	120 - NC	Jakob	09-30
134	192	180 - NC	Jakob	09-30
135	193	180 - NC	Jakob	09-30
136	194	240 - NC	Jakob	09-30
137	195	240 - NC	Jakob	09-30
138	196	360 - NC	Jakob	09-30
139	197	360 - NC	Jakob	09-30
140	198	480 - NC	Jakob	09-30
141	199	480 - NC	Jakob	09-30
142	200	600 - NC	Jakob	09-30
143	201	600 - NC	Jakob	09-30
144	202	Xyla Lin + His (purified)	Consti	10-03
145	203	Xyla Circ + His (purified)	Consti	10-03
146	204	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	Charlotte	10-03			1044C1-2
147	205	pSBX1K3-2Zv2Npu:LaLys+may1	Charlotte	10-03			1046C1-2
148	206	pSBX1K3-2Zv2Npu:LaLys+sgt2	Charlotte	10-03			1047C1-2
149	207	pSBX1K3-2Zv2Npu:LaLys+sho1	Charlotte	10-03			1048C1-2
150	208	pSBX1K3-2Zv2Npu:LaLys+sho2	Charlotte	10-03			1049C1-3
151	209	pSBX1K3-2Zv2Npu:LaLys+flexlinker	Charlotte	10-03			436C1-13
152	210	pSBX1K3-2Zv2Npu:LaLys+ord1	Charlotte	10-03			1114C1
153	211	pSBX1K3-2Zv2Npu:LaLys+ord3	Charlotte	10-03			1115C1
154	212	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	Charlotte	10-03			436C1-14
155	213	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	Charlotte	10-03			596C1-8
156	214	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	Charlotte	10-03			597C1-10
157	215	pSBX1K3-2Zv2Npu:LaLys+may1	Charlotte	10-03			1044C1-3
158	216	pSBX1K3-2Zv2Npu:LaLys+sgt2	Charlotte	10-03			1046C1-3
159	217	pSBX1K3-2Zv2Npu:LaLys+sho1	Charlotte	10-03			1047C1-3
160	218	pSBX1K3-2Zv2Npu:LaLys+sgt4	Charlotte	10-03			1018C1-1
161	219	pSBX1K3-2Zv2Npu:LaLys+flexlinker	Charlotte	10-03			1049C1-4
162	220	pSBX1K3-2Zv2Npu:LaLys+ord1	Charlotte	10-03			1114C1-2
163	221	pSBX1K3-2Zv2Npu:LaLys+ord3	Charlotte	10-03			1115C1-2
164	222
165	223
166	224
167	225
168	226
169	227
170	228
171	229
172	230
173	231
174	232
175	233
176	234
177	235
178	236
179	237
180	238
181	239
182	240
183	241
184	242
185	243
186	244
187	245
188	246
189	247
190	248
191	249
192	250
193	251
194	252
195	253
196	254
197	255
198	256
199	257
200	258
201	259
202	260
203	261
204	262
205	263
206	264
207	265
208	266
209	267
210	268
211	269
212	270
213	271
214	272
215	273
216	274
217	275
218	276
219	277
220	278
221	279
222	280
223	281
224	282
225	283
226	284
227	285
228	286
229	287
230	288
231	289
232	290
233	291
234	292
235	293
236	294
237	295
238	296
239	297
240	298
241	299
242	300
243	301
244	302
245	303
246	304
247	305
248	306
249	307
250	308
251	309
252	310
253	311
254	312
255	313
256	314
257	315
258	316
259	317
260	318
261	319
262	320
263	321
264	322
265	323
266	324
267	325
268	326
269	327
270	328
271	329
272	330
273	331
274	332
275	333
276	334
277	335
278	336
279	337
280	338
281	339
282	340
283	341
284	342
285	343
286	344
287	345
288	346
289	347
290	348
291	349
292	350
293	351
294	352
295	353
296	354
297	355
298	356
299	357
300	358
301	359
302	360
303	361
304	362
305	363
306	364
307	365
308	366
309	367
310	368
311	369
312	370
313	371
314	372
315	373
316	374
317	375
318	376
319	377
320	378
321	379
322	380
323	381
324	382
325	383
326	384
327	385
328	386
329	387
330	388
331	389
332	390
333	391
334	392
335	393
336	394
337	395
338	396
339	397
340	398
341	399
342	400
343	401
344	402
345	403
346	404
347	405
348	406
349	407
350	408
351	409
352	410
353	411
354	412
355	413
356	414
357	415
358	416
359	417
360	418
361	419
362	420
363	421
364	422
365	423
366	424
367	425
368	426
369	427
370	428
371	429
372	430
373	431
374	432
375	433
376	434
377	435
378	436
379	437
380	438
381	439
382	440
383	441
384	442
385	443
386	444
387	445
388	446
389	447
390	448
391	449
392	450
393	451
394	452
395	453
396	454
397	455
398	456
399	457
400	458
401	459
402	460
403	461
404	462
405	463
406	464
407	465
408	466
409	467
410	468
411	469
412	470
413	471
414	472
415	473
416	474
417	475
418	476
419	477

Team:Heidelberg/AwesomeSheet/Samples

From 2014.igem.org

– Samples

Awesome Sheet PA Gels Dnmt1 Methylation Assay Blots Waste SURPREMErun Strains Stocks Samples Purifications Plate Reader Marker LIGHTRun Enzymes Competent cells Induction Growth 3AL AGels AssmProd Assembly GoldenGate Parts Submission Parts PCR products PCRs Plasmids Plates Primer Res SL Trafos