Team:Heidelberg/AwesomeSheet/Purifications

From 2014.igem.org

– Purifications

	A	B	C	D	E	F	G	H	L	M	N	P	Q	R	S	T	AB	AD	AF	AG	AH	AI	AJ
1		präID
2	ID		MidnightDoc-ID	Date	Time	Eperimentator	Source		Method	Content				Solvent	V	c	left	Related Stock	Comment	2beUsed	Origin	Related sequenzing
3							Typ	ID		Type	ID	Plasmid-ID	Name		µl	ng/µl	V/µL		Comment			Barcode	Result

4	O1	O1		06-08	15:30	Jakob, Jan	Growth	37C2	Qiagen miniprep kit	Plasmid	1	1	BBa_J61002-BBa_J23110		50.0	242	48.0	11	lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA		D2014-4-19D	BF8244	ok
5	O2	O2		06-08	15:30	Jakob, Jan	Growth	38C2	Qiagen miniprep kit	Plasmid	2	2	BBa_J61002-BBa_J23118		50.0	419	45.6	12	lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA		D2014-4-21B	BF8245	ok
6	O3	O3		06-08	15:30	Jakob, Jan	Growth	39C2	Qiagen miniprep kit	Plasmid	3	3	BBa_J61002-BBa_J23100		50.0	239	43.8	13	lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA		D2014-4-17D	BF8246	ok
7	O4	O4		06-07	23:00	Silvan, Jakob and Max W.	Growth	40C1	Qiagen miniprep kit	Plasmid	4	4	pSB1C3-BBa_C0012		50.0	185	40.6	10	was accidantially switched		D2014-2-2N	BF8250	was prevoiously thought to be BBa_I15016
8	O5	O5		06-08	15:30	Jakob, Jan	Growth	41C1	Qiagen miniprep kit	Plasmid	5	5	pSB1C3-BBa_E0240		50.0	248	36.9	14	lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA		D2014-2-24B	BF8248	insertion before prefix leading to additional EcoRI site
9	O6	O6		06-07	23:00	Silvan, Jakob and Max W.	Growth	42C1	Qiagen miniprep kit	Plasmid	6	6	pSB1C3-BBa_R0010		50.0	119	37.6	8			D2014-3-4G	BF8249	ok
10	O7	O7		06-07	23:00	Silvan, Jakob and Max W.	Growth	43C1	Qiagen miniprep kit	Plasmid	7	7	pSB1C3-BBa_I15016		50.0	145	41.1	7	was accidentially switched		D2014-3-17E	BF8247	was previously thought to be BBa_C0012
11	O8	O8		06-08	15:30	Jakob, Jan	Growth	44C1	Qiagen miniprep kit	Plasmid	8	8	pSB1C3-BBa_I15017		50.0	193	37.6	15	lysis was performed in the centrifuge tube leading to a potentially huge loss of DNA		D2014-3-17G	BF8251	ok
12	O12	O12		06-07	23:00	Silvan, Jakob and Max W.	Growth	10C1	Qiagen miniprep kit	Plasmid	12	12	pUC19		50.0	216	48.0	9			O12-2
13	O12-2	O12	s/vSuyTkAuEqcHYkHK6	06-04			Extern	Invitrogen		Plasmid	12	12	pUC19		50.0	0	28.0
14	O13	O13		06-12	16:00	Jakob	Growth	10	Qiagen miniprep kit	Plasmid	13	13	pSB1C3-pKH6insert		50.0	102	47.0	16				BF8252, BF8253	ok, ok
15	O14	O14		06-12	16:00	Jakob	Growth	11	Qiagen miniprep kit	Plasmid	14	14	pSB1C3-pKH7insert		50.0	95	48.0	17				BF8253, BF8254	ok, ok
16	O15	O15		06-12	16:00	Jakob	Growth	12	Qiagen miniprep kit	Plasmid	15	15	pSB1C3-pKH18insert		50.0	125	48.0	18				BF8254, BF8255	negativ, araC, negativ, Shewanella violacea
17	O12-3	O12		06-18	15:00	Jakob	Purification	O12	Dilution	Plasmid	12	12	pUC19		1,000.0	1	1,000.0
18	O12-4	O12		06-18	15:00	Jakob	Purification	O12-3	Dilution	Plasmid	12	12	pUC19		1,000.0	0	1,000.0
19	O16	O16		06-22	0:40	Silvan	Growth	52C1	Qiagen miniprep kit	Plasmid	16	16	pGBMCS-SortA		50.0	59	41.6	19			O12-2	II2247	ok
20	O17	O17		06-22	0:40	Silvan	Growth	53C1	Qiagen miniprep kit	Plasmid	17	17	pHYRSF1		50.0	327	46.2	20			O12-2	II2232	ok
21	O18	O18		06-22	0:40	Silvan	Growth	54C1	Qiagen miniprep kit	Plasmid	18	18	pHYRSF1-02		50.0	263	45.1	21			O12-2	II2233	ok
22	O19	O19		06-22	0:40	Silvan	Growth	55C1	Qiagen miniprep kit	Plasmid	19	19	pJJDuet30		50.0	297	49.2	22			O12-2	II2234	Primer hat an 2 Stellen gebunden, sieht aber ok aus
23	O20	O20		06-22	0:40	Silvan	Growth	56C1	Qiagen miniprep kit	Plasmid	20	20	pSFBAD09		50.0	60	45.8	23			D2014-4-19D	II2235	ok
24	O22	O22		06-25	12:00	Maggie, Silvan	Growth	57C1	Qiagen miniprep kit	Plasmid	22	22	pFastBac-mDNMT1		50.0	234	48.9	24			O22-0
25	O23	O23		06-25	12:00	Maggie, Silvan	Growth	58C1	Qiagen miniprep kit	Plasmid	23	23	pRSFDuet-1-mDNMT1(731-1602)		50.0	191	44.8	25			O23-0	II2225, II2227, II2228, II2229	ok, ok, ok, ok
26	O24	O24		06-25	12:00	Maggie, Silvan	Growth	59C1	Qiagen miniprep kit	Plasmid	24	24	pIT77		50.0	52	45.2	26			O24-0	II2236	ok
27	O25	O25		06-25	12:00	Maggie, Silvan	Growth	60C1	Qiagen miniprep kit	Plasmid	25	25	pIT29		50.0	78	46.8	27			O25-0	II2237	ok
28	O26	O26		06-25	12:00	Maggie, Silvan	Growth	61C1	Qiagen miniprep kit	Plasmid	26	26	pIT21		50.0	62	46.0	28			O26-0	II2238	ok
29	O27	O27		06-25	12:00	Maggie, Silvan	Growth	62C1	Qiagen miniprep kit	Plasmid	27	27	pCL20		50.0	41	43.8	29			O27-0	II2239	ok
30	O28	O28		06-25	12:00	Maggie, Silvan	Growth	63C1	Qiagen miniprep kit	Plasmid	28	28	pAU07		50.0	71	43.0	30			O28-0	II2240	ok
31	O29	O29		06-25	12:00	Maggie, Silvan	Growth	64C1	Qiagen miniprep kit	Plasmid	29	29	pVS41		50.0	115	0	31			O29-0	II2241	ok
32	O30	O30		06-25	12:00	Maggie, Silvan	Growth	65C1	Qiagen miniprep kit	Plasmid	30	30	pVS07		50.0	152	46.7	32			O30-0	II2242	ok
33	O31	O31	-	06-26	1:00	Silvan	Growth	66C1	Qiagen miniprep kit	Plasmid	31	31	pRARE			75	0.0	33			D2014-4-21B	II2224	ok, aber falsche Richtung
34	O5-2	O5		06-28	13:00	Constantin	Purification	O5	Dilution	Plasmid	5	5	pSB1C3-BBa_E0240		248.0	1	239.0
35	O18-2	O18		06-28	13:00	Constantin	Purification	O18	Dilution	Plasmid	18	18	pHYRSF1-02		102.0	1	99.0
36	O13-2	O13		06-28	13:00	Constantin	Purification	O13	Dilution	Plasmid	13	13	pSB1C3-pKH6insert		326.0	1	0		discarded
37	O17-2	O17		06-28	13:00	Constantin	Purification	O17	Dilution	Plasmid	17	17	pHYRSF1		262.0	1	260.0
38	O19-2	O19		06-30	16:10	Jan	Purification	O19	Dilution	Plasmid	19	19	pJJDuet30		297.0	1	296.0
39	O20-2	O20		06-30	16:15	Max	Purification	O20	Dilution	Plasmid	20	20	pSFBAD09		59.5	1	56.5
40	P4	P4		07-01	17:00	Charlotte, Constantin	Gel	7-2	Gel extraction	PP	4		pSB1C3_NpuDnaE-N_FLAG-NpuDnaE-C		50.0	14	50.0
41	P1	P1		07-01	17:00	Charlotte, Constantin	Gel	7-3	Gel extraction	PP	1		NpuDnaE-C_pSB1C3-FLAG_6His-BsaI-ccdB		50.0	9	50.0
42	P2	P2		07-01	17:00	Charlotte, Constantin	Gel	7-4	Gel extraction	PP	2		ccdB_NpuDnaE-C-6His-BsaI_BasI-NpuDnaE-N		50.0	40	50.0
43	P3	P3		07-01	17:00	Charlotte, Constantin	Gel	7-5	Gel extraction	PP	3		NpuDnaE-N_BsaI-ccdB_pSB1C3		50.0	12	50.0
44	P5	P5		07-01	17:00	Charlotte, Constantin	Gel	7-6	Gel extraction	PP	5		NpuDnaE-C_pSB1C3-FLAG_BsaI-ccdB		50.0	20	50.0
45	P7	P7		07-01	17:00	Charlotte, Constantin	Gel	7-7	Gel extraction	PP	7		pSB1C3_SspDnaE-N-taataat_Flag-SspDnaE_C		50.0	45	50.0
46	P9	P9		07-01	17:00	Charlotte, Constantin	Gel	7-8	Gel extraction	PP	9		ccdB_SspDnaE-C-BsaI_BsaI-SspDnaE-N		50.0	65	50.0
47	P10	P10		07-01	17:00	Charlotte, Constantin	Gel	7-9	Gel extraction	PP	10		SspDnaE-N_ccdB-BsaI_taataat-pSB1C3		50.0	64	50.0
48	P12	P12		07-01	17:00	Charlotte, Constantin	Gel	7-10	Gel extraction	PP	12		ccdB_SspDnaE-C-6His-BsaI_BsaI-SspDnaE-N		50.0	52	50.0
49	P8	P8		07-01	17:00	Charlotte, Constantin	Gel	7-11	Gel extraction	PP	8		SspDnaE-C_pSB1C3-Flag_BsaI-ccdB		50.0	27	50.0
50	P6	P6		07-01	17:00	Charlotte, Constantin	Gel	7-12	Gel extraction	PP	6		ccdB_NpuDnaE-C-BsaI _BasI-NpuDnaE-N		50.0	53	50.0
51	P11	P11		07-01	17:00	Charlotte, Constantin	Gel	7-13	Gel extraction	PP	11		SspDnaE-C_pSB1C3-Flag_6His-BsaI-ccdB		50.0	31	50.0
52	O36	O36		07-03	23:00	Lisa	Growth	130C1	Qiagen miniprep kit	Plasmid	36	36	pSB1AK8-BBa_I712074		50.0	58	0	50			D2013-5-6N	II2226	RFC23 (missing the final G in the prefix and the primary T in the suffix)
53	O35	O35		07-03	23:00	Lisa	Growth	129C2	Qiagen miniprep kit	Plasmid	35	35	pSB1C3-2ZSspH6		50.0	66	0		discarded		A35
54	O35-2	O35		07-03	23:00	Lisa	Growth	129C3	Qiagen miniprep kit	Plasmid	35	35	pSB1C3-2ZSspH6		50.0	106	29.1	47			A35	II2230, II2243	ok, ok
55	O35-3	O35		07-03	23:00	Lisa	Growth	129C4	Qiagen miniprep kit	Plasmid	35	35	pSB1C3-2ZSspH6		50.0	106	0		discarded		A35	II2231, II2244	ok, ok
56	O35-4	O35		07-03	23:00	Lisa	Growth	129C5	Qiagen miniprep kit	Plasmid	35	35	pSB1C3-2ZSspH6		50.0	56	0		discarded		A35
57	O35-5	O35		07-03	23:00	Lisa	Growth	129C6	Qiagen miniprep kit	Plasmid	35	35	pSB1C3-2ZSspH6		50.0	117	0		discarded		A35
58	O16-2	O16		07-04	12:45	Silvan	Growth	52C2	Qiagen miniprep kit	Plasmid	16	16	pGBMCS-SortA		50.0	77	50.0				O12-2
59	O23-2	O23		07-04	12:45	Silvan	Growth	131C1	Qiagen miniprep kit	Plasmid	23	23	pRSFDuet-1-mDNMT1(731-1602)		50.0	514	47.0	40	discarded		O23
60	O38	O38		07-06	23:19	Max H.	Growth	153C1	Qiagen miniprep kit	Plasmid	38	38	pSB1AK8-BBa_I712074-BBa_I15016		50.0	38	50.0	34			S38
61	O39	O39		07-06	23:19	Max H.	Growth	154C1	Qiagen miniprep kit	Plasmid	39	39	pSB1AK8-BBa_I712074-BBa_I15017		50.0	58	50.0	35			S39
62	O40	O40		07-06	23:19	Max H.	Growth	155C1	Qiagen miniprep kit	Plasmid	40	40	pSB1AK8-BBa_I712074-BBa_E0240		50.0	51	50.0	36			S40
63	O41	O41		07-06	23:19	Max H.	Growth	156C2	Qiagen miniprep kit	Plasmid	41	41	pSB1AK8-BBa_I712074-S-BBa_J70390		50.0	68	50.0	37			S41
64	O37	O37		07-06	23:19	Max H.	Growth	142C1	Qiagen miniprep kit	Plasmid	37	37	pSB1A2-BBa_S03520		50.0	174	50.0	38			D2013-5-7J
65	P18	P18		07-06	23:20	Jan	PCR	36	PCR purification kit	PP	18		BBa_E0040_EcoR1_Spe1		30.0	38	3.3
66	O32	O32		07-07	22:00	Carolin	Growth	157C1	Qiagen miniprep kit	Plasmid	32	32	pSB1C3-2ZNpuH6		50.0	78	0		discarded		A32#2
67	O32-2	O32		07-07	22:00	Carolin	Growth	157C2	Qiagen miniprep kit	Plasmid	32	32	pSB1C3-2ZNpuH6		50.0	124	0	39	discarded		A32#2	II2245, II2246	insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part, insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part
68	O32-3	O32		07-07	22:00	Carolin	Growth	157C3	Qiagen miniprep kit	Plasmid	32	32	pSB1C3-2ZNpuH6		50.0	66	0		discarded		A32#2
69	O33	O33		07-07	22:00	Carolin	Growth	158C1	Qiagen miniprep kit	Plasmid	33	33	pSB1C3-2ZNpu		50.0	96	39.6	42	use as O32		A33	II2248, II2249	insertion in the prefix, additional His after Flag, bad sequencing
70	O33-2	O33		07-07	22:00	Carolin	Growth	158C2	Qiagen miniprep kit	Plasmid	33	33	pSB1C3-2ZNpu		50.0	66	34.7	42b	discarded		A33	II2252	additional His after flag, insertion in the prefix
71	O33-3	O33		07-07	22:00	Carolin	Growth	158C3	Qiagen miniprep kit	Plasmid	33	33	pSB1C3-2ZNpu		50.0	71	35.9	42c	discarded		A33	II2253	additional His after flag, insertion in the prefix
72	R18	R18		07-05	18:00	Jan	Gel	13-2	Gel extraction	Restriction	18		pSB1C3-BBa_E0240 (X/P)		20.0	14	15.7
73	R19	R19		07-05	18:00	Jan	Gel	13-3	Gel extraction	Restriction	19		pSB1C3-BBa_I15016 (X/P)		20.0	9	15.7
74	R20	R20		07-05	18:00	Jan	Gel	13-4	Gel extraction	Restriction	20		pSB1C3-BBa_I15017 (X/P)		20.0	14	15.7		O2
75	R21	R21		07-05	18:00	Jan	Gel	13-5	Gel extraction	Restriction	21		BBa_J61002-BBa_J23100 (S/P)		20.0	18	15.7
76	R38	R38		07-05	18:00	Jan	Restriction	38	PCR purification kit	Restriction	38		pSB1AK8-BBa_I712074 (S/P)		20.0	12	13.1
77	O43	O43		07-08	17:13	Silvan	Growth	164C1	Qiagen miniprep kit	Plasmid	43	43	pSB1C3-BBa_I746907	H2O	50.0	39	23.4	46			D2014-3-12I	II2250, II2251	,
78	O29,30,31	P		07-08	17:13	Silvan	Growth	161C1	Qiagen miniprep kit	Plasmids				H2O	50.0	58	50.0				O30
79	O29,30,31-2	P		07-08	17:13	Silvan	Growth	161C2	Qiagen miniprep kit	Plasmids				H2O	50.0	91	50.0				O30
80	O29,30,31-3	P		07-08	17:13	Silvan	Growth	162C1	Qiagen miniprep kit	Plasmids				H2O	50.0	116	50.0	45			O29
81	O29,30,31-4	P		07-08	17:13	Silvan	Growth	162C2	Qiagen miniprep kit	Plasmids				H2O	50.0	112	50.0				O29
82	O42	O42		07-09	15:00	Silvan	Growth	165C1	Qiagen miniprep kit	Plasmid	42	42	pSB1A2-BBa_I719005		50.0	205	45.1				D2013-5-15N
83	R44	R44		07-08	21:00	Charlotte	Gel	18-3	Gel extraction	Restriction	44		pSB1C3-2ZSspH6 (BsaI)				-6.0
84	R45	R45		07-08	21:00	Charlotte	Gel	18-2	Gel extraction	Restriction	45		pSB1C3-2ZNpuH6 (BsaI)				-6.0
85	R46	R46		07-08	21:00	Charlotte	Gel	18-4	Gel extraction	Restriction	46		BBa_E0040_EcoR1_Spe1 (E/S)				-4.0
86	R44-2	R44		07-09	21:00	Charlotte	Restriction	44	PCR purification kit	Restriction	44		pSB1C3-2ZSspH6 (BsaI)	H2O	#N/A	#N/A	#N/A
87	R45-2	R45		07-09	21:00	Charlotte	Restriction	45	PCR purification kit	Restriction	45		pSB1C3-2ZNpuH6 (BsaI)	H2O	#N/A	#N/A	#N/A
88	R46-2	R46		07-09	21:00	Charlotte	Restriction	46	PCR purification kit	Restriction	46		BBa_E0040_EcoR1_Spe1 (E/S)	H2O	#N/A	#N/A	#N/A
89	P18-2	P18		07-10	19:30	Lisa	PCR	44	PCR purification kit	PP	18		BBa_E0040_EcoR1_Spe1		30.0	80	17.5
90	R52	R52		07-10		Silvan	Restriction	52	PCR purification kit	Restriction	52		pSB1C3-2ZNpu (X/P)		#N/A	#N/A	#N/A
91	R53	R53		07-10		Silvan	Restriction	53	PCR purification kit	Restriction	53		pSB1C3-2ZNpu (X/P)		#N/A	#N/A	#N/A
92	R54	R54		07-10		Silvan	Restriction	54	PCR purification kit	Restriction	54		pSB1C3-2ZNpu (X/P)		#N/A	#N/A	#N/A
93	R55	R55		07-10		Silvan	Restriction	55	PCR purification kit	Restriction	55		pSB1AK8-BBa_I712074 (S/P)		#N/A	#N/A	#N/A
94	R56	R56		07-12	14:00	Lisa + Consti	Gel	21-2	Gel extraction	Restriction	56		pSB1C3-2ZSspH6 (BsaI)	H2O	30.0	12	26.7
95	R57	R57		07-12	14:00	Lisa + Consti	Gel	21-3	Gel extraction	Restriction	57		pSB1C3-2ZNpuH6 (BsaI)	H2O	30.0	18	26.8
96	R58	R58		07-12	14:00	Lisa + Consti	Gel	21-4	Gel extraction	Restriction	58		BBa_E0040_EcoR1_Spe1 (E/S)	H2O	30.0	35	23.8
97	O47	O47		07-12	14:35	Silvan	Growth	178C1	Qiagen miniprep kit	Plasmid	47	47	pSB1AK8-T7-2ZNpu		50.0	346	47.0	54			S47#3	II2263, II2265	,
98	P24	P24		07-12	18:05	Silvan	PCR	57	PCR purification kit	PP	24		BBa_746907_BsaI=EcoR1_His6_BsaI=Spe1		30.0	135	27.0
99	P25	P25	x/YYEs6gNX8ewyXfWGv	07-12	17:00	Silvan	Oligo		Annealing	PP	25		BsaI=EcoRI_null_BsaI=SpeI		100.0	973	97.0
100	O48	O48		07-14	20:30	Silvan	Growth	189C1	Qiagen miniprep kit	Plasmid	48	48	pSB1AK8-T7-2ZNpu:(GFP-H6)		50.0	284	49.0				G48	II2264, II2266	,
101	O45	O45		07-15	1:00	Lisa	Growth	181C1	Qiagen miniprep kit	Plasmid	45	45	pSB1C3-2ZNpuH6:GFP	EB	50.0	10	0		vertauschaungen!		S45#4
102	O45-2	O45		07-15	1:00	Lisa	Growth	181C2	Qiagen miniprep kit	Plasmid	45	45	pSB1C3-2ZNpuH6:GFP		50.0	7	0		vertauschaungen!, really bad, repeat, probaBIy not enough bacteria..		S45#4
103	O45-3	O45		07-15	1:00	Lisa	Growth	181C3	Qiagen miniprep kit	Plasmid	45	45	pSB1C3-2ZNpuH6:GFP		50.0	11	0		vertauschaungen!		S45#4
104	O44	O44		07-15	1:00	Lisa	Growth	182C3	Qiagen miniprep kit	Plasmid	44	44	pSB1C3-2ZSspH6:GFP		50.0	20	0		vertauschaungen!		S44#4
105	O44-2	O44		07-15	1:00	Lisa	Growth	182C4	Qiagen miniprep kit	Plasmid	44	44	pSB1C3-2ZSspH6:GFP		50.0	9	0		vertauschaungen!		S44#4
106	O44-3	O44		07-15	1:00	Lisa	Growth	182C5	Qiagen miniprep kit	Plasmid	44	44	pSB1C3-2ZSspH6:GFP		50.0	5	0		vertauschaungen!		S44#4
107	O45-4	O45		07-15	16:30	Lisa	Growth	181C1	Qiagen miniprep kit	Plasmid	45	45	pSB1C3-2ZNpuH6:GFP	EB	30.0	177	0		vertauschaungen!		S45#4
108	O44-4	O45		07-15	16:30	Lisa	Growth	181C2	Qiagen miniprep kit	Plasmid	44	45	pSB1C3-2ZSspH6:GFP		30.0	219	0		vertauschaungen! previously O45-5, use 44-8		S45#4	II2255, II2254	no sequencing, we realized that "ist glaube ich Ssp_DnaE. hat funktioniert", besides that ok
109	O45-6	O45		07-15	16:30	Lisa	Growth	181C3	Qiagen miniprep kit	Plasmid	45	45	pSB1C3-2ZNpuH6:GFP		30.0	92	0		vertauschaungen!		S45#4
110	O45-5	O44		07-15	16:30	Lisa	Growth	182C3	Qiagen miniprep kit	Plasmid	45	44	pSB1C3-2ZNpuH6:GFP		30.0	167	0		vertauschaungen! previously O44-4, use 45-8		S44#4	II2257, II2256	no sequencing, we realized that this is npu (O45), has a mutation A1197del
111	O44-5	O44		07-15	16:30	Lisa	Growth	182C4	Qiagen miniprep kit	Plasmid	44	44	pSB1C3-2ZSspH6:GFP		30.0	88	0		vertauschaungen!		S44#4
112	O44-6	O44		07-15	16:30	Lisa	Growth	182C5	Qiagen miniprep kit	Plasmid	44	44	pSB1C3-2ZSspH6:GFP		30.0	166	0		vertauschaungen!		S44#4
113	R61	R61	x/MRmcQNuEBtR8vQfuH	07-14	23:00	Max W	Oligo		Annealing	Restriction	61		pSBX-T7	T4Ligase Buffer	100.0	407	46.0		cycler run 20
114	P30	P30	s/3cByz57n4waxPDtB8				Distibution	2014		PP	30		pSB1A3			25	-12
115	P31	P31	s/HuGEqnxD4fRJekJnM				Distibution	2014		PP	31		pSB1C3			25	-25
116	P32	P32	s/qMGcNDnLGXXDM2e2o				Distibution	2014		PP	32		pSB1K3			25	-25
117	P33	P33	s/jhioKQSAgk9otaes2				Distibution	2014		PP	33		pSB1T3			25	-25
118	O50	O50	s/wnu2JTHbc5gqv4h3A	07-15	18:00	Jan	Extern	O2	from SYNtheSYS	Plasmid	50	50	pSB4A5-BBa_J04450			157	-12.74
119	O51	O51	s/SHQw48zRpXSsXpDBA	07-15	18:00	Jan	Extern	O17	from SYNtheSYS	Plasmid	51	51	pSB4C5-BBa_J04450			529	-5.67
120	O52	O52	s/yjpzG9aJGnavCw7jQ	07-15	18:00	Jan	Extern	O3	from SYNtheSYS	Plasmid	52	52	pSB4K5-BBa_J04450			102	-17.8
121	O53	O53	s/xK8jaWZ8EBRrD6vJJ	07-15	18:00	Jan	Extern	O12	from SYNtheSYS	Plasmid	53	53	pSB1A2-BBa_I13507			218	-30.7
122	O49	O49	s/xjP79KGLhyNgMWQLb	07-15	18:00	Jan	Extern	O27	from SYNtheSYS	Plasmid	49	49	pSB1A3-BBa_J04450			132	0
123	O54	O54	s/jTBLe2Z37PN669Ti2	07-15	18:00	Jan	Extern	O28	from SYNtheSYS	Plasmid	54	54	pSB1K3-BBa_J04450			76	-26.3		concentration not realistic, see Gel 36
124	O65	O65	s/RseaZHbrsPG9KSpL2	07-15	18:00	Jan	Extern	O15	from SYNtheSYS	Plasmid	65	65	pSB1C3-BBa_J04450			321	0.0		doesnt exist
125	O64	O64	s/gCzWyGy7AuyfqJbkB	07-15	18:00	Jan	Extern	O16	from SYNtheSYS	Plasmid	64	64	pSB1T3-BBa_J04450		50.0	269	38.8
126	O64-2	O64	s/uqoZiCdHqbkddkxGq	07-23	11:11	Jan	Extern	O16b	from SYNtheSYS	Plasmid	64	64	pSB1T3-BBa_J04450		50.0	137	35.4
127	O64-3	O64	s/YeXamSC7qXWnA2j8L	07-23	11:11	Jan	Extern	O16'	from SYNtheSYS	Plasmid	64	64	pSB1T3-BBa_J04450		50.0	149	36.5
128	R62	R62		07-16	18:00	Caro	Gel	27-2-2,27-3-2	Gel extraction	Restriction	62		pSB1A2-BBa_I13507 (X/P)	EB	30.0	8	-6.0		estimated
129	R63	R63		07-16	18:00	Caro	Restriction	63	PCR purification kit	Restriction	63			EB	30.0	2	12.3
130	R64	R64		07-16	18:00	Caro	Restriction	64	PCR purification kit	Restriction	64		0,	EB	30.0	6	7.5
131	R65	R65		07-16	18:00	Caro	Restriction	65	PCR purification kit	Restriction	65		pSB1K3 (E/P)	EB	30.0	19	21.5
132	R66	R66		07-16	18:00	Caro	Restriction	66	PCR purification kit	Restriction	66		pSB1T3 (E/P)	EB	30.0	8	21.6
133	R67	R67		07-16	18:00	Caro	Gel	26-2-1,26-3-1	Gel extraction	Restriction	67		pSB4A5-BBa_J04450 (E/P)	EB	30.0	44	20.7
134	R68	R68		07-16	18:00	Caro	Gel	26-7-1,26-8-1	Gel extraction	Restriction	68		pSB4C5-BBa_J04450 (E/P)	EB	30.0	38	20.7
135	R69	R69		07-16	18:00	Caro	Gel	26-4-1,26-5-1,26-6-1	Gel extraction	Restriction	69		pSB4K5-BBa_J04450 (E/P)	EB	30.0	23	20.8
136	R70	R70		07-16	18:00	Caro	Gel	26-9-1,26-10-1	Gel extraction	Restriction	70		pSB1A3-BBa_J04450 (E/P)	EB	30.0	33	21.4
137	R71	R71		07-16	18:00	Caro	Gel	26-11-1,26-12-1	Gel extraction	Restriction	71		pSB1K3-BBa_J04450 (E/P)	EB	30.0	21	21.2
138	R72	R72		07-17	18:00	Caro	Gel	28-2-2,28-3-2	Gel extraction	Restriction	72		pSB1A2-BBa_I13507 (X/P)	EB	50.0	5	-15.3
139	O23-3	O23		07-17	18:45	Maggie	Purification	O23	Dilution	Plasmid	23	23	pRSFDuet-1-mDNMT1(731-1602)		191.0	1	160.0		missing
140	O49-2	O49		07-17	18:45	Maggie	Purification	O49	Dilution	Plasmid	49	49	pSB1A3-BBa_J04450		131.5	1	126.5
141	R61-2	R61		07-18	1:50	Silvan	Purification	R61	Dilution	Restriction	61		T7prom	H2O	100.0	4	87.24419514
142	P36	P36		07-20	15:00	Maggie	Gel	32-3-2.2kb,32-3-2.2kb	Gel extraction	PP	36		pSB1A3_SortAc-H6_Smt3		30.0	24	29.0		342
143	P35	P35		07-20	15:00	Maggie	Gel	32-6-778bp,32-7-778bp	Gel extraction	PP	35		ccdB_Smt3-TEVc-BsaI_SortAc-H6-RFC10suffix		30.0	3	29.0		778
144	P34	P34		07-20	15:00	Maggie	Gel	32-9-342bp,32-10-342bp	Gel extraction	PP	34		Smt3_RFC10prefix_TEVc-BsaI		30.0	58	29.0		2207
145	P36-2	P36		07-20		Maggie	Gel	34-1-2.2kb,34-3-2.2kb	Gel extraction	PP	36		pSB1A3_SortAc-H6_Smt3		30.0	24	29.0
146	P35-2	P35		07-20		Maggie	Gel	34-4-778bp,34-5-778bp	Gel extraction	PP	35		ccdB_Smt3-TEVc-BsaI_SortAc-H6-RFC10suffix		30.0	32	29.0
147	P34-2	P34		07-20		Maggie	Gel	34-6-342bp,34-7-342bp	Gel extraction	PP	34		Smt3_RFC10prefix_TEVc-BsaI		30.0	57	29.0
148	O59	O59		07-20	12:00	Caro	Growth	205C4	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O		144	0				L59#3	II2258	bad sequencing
149	O58	O58		07-20	12:00	Caro	Growth	194C1	Qiagen miniprep kit	Plasmid	58	58	pSBX1C3-BBa_I13507	H2O		100	0				L58	II2259	bad sequencing
150	O46	O46		07-20	12:00	Caro	Growth	175C1-1	Qiagen miniprep kit	Plasmid	46	46	pSB1C3-BBa_S03518	H2O		104	0				D2014-3-21G
151	O57	O57		07-20	12:00	Caro	Growth	203C1	Qiagen miniprep kit	Plasmid	57	57	pSBX1A3-BBa_I13507	H2O		130	0				L57#3	II2260	bad sequencing
152	O36-2	O36		07-20	12:00	Caro	Growth	180C2-2	Qiagen miniprep kit	Plasmid	36	36	pSB1AK8-BBa_I712074	H2O		122	0.0				O36
153	O59-2	O59		07-20	12:00	Caro	Growth	195C1	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O		76	0.0				L59	II2261	bad sequencing
154	O58-2	O58		07-20	12:00	Caro	Growth	204C2	Qiagen miniprep kit	Plasmid	58	58	pSBX1C3-BBa_I13507	H2O		134	0				L58#2	II2262	pre insert insert containing ecoRI site
155	O22-0		x/8hoekt4dMb7JGqjEL	06-23	12:00	Maggie, Silvan	Extern	Bashtrykov		Plasmid	22	22	pFastBac-mDNMT1				0.0
156	O23-0		x/tDBSAGTGnyvvt7Yha	06-23	12:00	Maggie, Silvan	Extern	Bashtrykov		Plasmid	23	23	pRSFDuet-1-mDNMT1(731-1602)				0.0
157	O24-0		s/jbP73zoHFnALQE7MF	06-23	12:00	Maggie, Silvan	Extern	Mootz		Plasmid	24	24	pIT77				0.0
158	O25-0		s/aEM4DJSRXsAcMeANd	06-23	12:00	Maggie, Silvan	Extern	Mootz		Plasmid	25	25	pIT29				0.0
159	O26-0		s/LsHLEn8gCMyhaQg2a	06-23	12:00	Maggie, Silvan	Extern	Mootz		Plasmid	26	26	pIT21				0.0
160	O27-0		s/zoGDXDeT9Rj2RJ5gm	06-23	12:00	Maggie, Silvan	Extern	Mootz		Plasmid	27	27	pCL20				0.0
161	O28-0		s/XzdfJqK3fgcXdKH53	06-23	12:00	Maggie, Silvan	Extern	Mootz		Plasmid	28	28	pAU07				0.0
162	O29-0		s/ajbhi77D2HWANBKqr	06-23	12:00	Maggie, Silvan	Extern	Mootz		Plasmid	29	29	pVS41				0.0
163	O30-0		s/CsQ3wGyhTFiLRGJPG	06-23	12:00	Maggie, Silvan	Extern	Mootz		Plasmid	30	30	pVS07				0.0
164	O55	O55		07-22	16:30	Silvan	Growth	211C2	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	310	-6.9	56			A55	II2267, II2268	,
165	R73	R73		07-23	1:40	Jan	Gel	36-2-1	Gel extraction	Restriction	73		pSB1A3-BBa_J04450 (E/P)	EB	30.0	6	23.5		22.28081059
166	R74	R74		07-23	1:40	Jan	Gel	36-3-1	Gel extraction	Restriction	74		pSB1C3-BBa_I15017 (E/P)	EB	30.0	11	25.0		24.53769559
167	R75	R75		07-23	1:40	Jan	Gel	36-4-1	Gel extraction	Restriction	75		pSB1K3-BBa_J04450 (E/P)	EB	30.0	3	22.4		2
168	R76	R76		07-23	1:40	Jan	Gel	36-5-1	Gel extraction	Restriction	76		pSB1T3-BBa_J04450 (E/P)	EB	30.0	8	24.0		is it really all 1T3 ? 22.4462776250127
169	R77	R77		07-23	1:40	Jan	Gel	36-6-1	Gel extraction	Restriction	77		pSB4A5-BBa_J04450 (E/P)	EB	30.0	10	23.7		25.39364231
170	R78	R78		07-23	1:40	Jan	Gel	36-7-1	Gel extraction	Restriction	78		pSB4C5-BBa_J04450 (E/P)	EB	30.0	9	23.5		25.35095579
171	R79	R79		07-23	1:40	Jan	Gel	36-8-1	Gel extraction	Restriction	79		pSB4K5-BBa_J04450 (E/P)	EB	30.0	11	24.0		25.02719503
172	R80	R80		07-23	1:40	Jan	Gel	36-9-2,36-10-2	Gel extraction	Restriction	80		pSB1A2-BBa_I13507 (X/P)	EB	30.0	9	-1.5		24.4047619
173	R77-2	R77		07-23	1:40	Jan	Gel	36-6-2,36-7-2,36-8-2	Gel extraction	Restriction	77		pSB4A5-BBa_J04450 (E/P)	EB	30.0	8	28.0		23.81907308
174	R83	R83		07-24	18:00	Jan	Gel	38-2-1,38-3-1	Gel extraction	Restriction	83		pSB1T3-BBa_J04450 (E/S)	EB	30.0	10	24.4		nanodrop did measure 75 ng/µL 13.943342776204
175	R84	R84		07-24	18:00	Jan	Gel	38-4-1,38-5-2	Gel extraction	Restriction	84		pSB1T3-BBa_J04450 (E/P)	EB	30.0	10	28.0		13.94
176	O55-2	O55		07-24	18:38	Silvan	Growth	209C2	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	86	48.0	57			A55	II2269
177	O55-3	O55		07-24	18:38	Silvan	Growth	211C1	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	292	48.0	58			A55	II2270
178	O55-4	O55		07-24	18:38	Silvan	Growth	212C1	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	198	27.8	59			A55#2	II2271
179	O49-3	O49		07-24	23:00	Silvan	Growth	217C1	Qiagen miniprep kit	Plasmid	49	49	pSB1A3-BBa_J04450	EB	50.0	110	48.0	62			O49
180	O50-2	O50		07-24	23:00	Silvan	Growth	218C1	Qiagen miniprep kit	Plasmid	50	50	pSB4A5-BBa_J04450	EB	50.0	107	48.0	61			O50
181	O51-2	O51		07-24	23:00	Silvan	Growth	219C1	Qiagen miniprep kit	Plasmid	51	51	pSB4C5-BBa_J04450	EB	50.0	311	25.5	63			O51
182	O52-2	O52		07-24	23:00	Silvan	Growth	220C1	Qiagen miniprep kit	Plasmid	52	52	pSB4K5-BBa_J04450	EB	50.0	53	47.8	64			O52
183	O53-2	O53		07-24	23:00	Silvan	Growth	221C1	Qiagen miniprep kit	Plasmid	53	53	pSB1A2-BBa_I13507	EB	50.0	129	32.5	60			O53
184	O54-2	O54		07-24	23:00	Silvan	Growth	222C1	Qiagen miniprep kit	Plasmid	54	54	pSB1K3-BBa_J04450	EB	50.0	103	48.0	65			O54
185	O66	O66		07-24	23:00	Silvan	Growth	224C1	Qiagen miniprep kit	Plasmid	66	66	pSB3T5-BBa_J04450	EB	50.0	129	30.5	67			D2014-4-8D
186	O64-4	O64		07-24	23:00	Silvan	Growth	227C1	Qiagen miniprep kit	Plasmid	64	64	pSB1T3-BBa_J04450	EB	50.0	85	24.5	66			O64-2
187	R85	R85	x/gfsFTzZFZNTLMd4RX	07-25	20:00	MaxW	Oligo		Annealing	Restriction	85		T7RBS_BioBrick_v2	T4L	1,000.0	32	999.0		cycler run 27, prev thought to be
188	R86	R86	x/sLge6jeo2xtv3ptz5	07-25	20:00	MaxW	Oligo		Annealing	Restriction	86		T7RBS-IGEMHD_v3	T4L	1,000.0	52	980.0		cycler run 28
189	R87	R87	x/wnWjSFKNHbx3ghRsj	07-25	20:00	MaxW	Oligo		Annealing	Restriction	87		AssemBIyIntN-linker	T4L	1,000.0	10	1,000.0		cycler run 29
190	R88	R88	x/Fbi9JGJKiYiRADvWx	07-25	20:00	MaxW	Oligo		Annealing	Restriction	88		AssemBIyIntC-linker	T4L	1,000.0	10	1,000.0		cycler run 30
191	O57-2	O57		07-26	14:50	Jan, Max H	Growth	234C1	Qiagen miniprep kit	Plasmid	57	57	pSBX1A3-BBa_I13507	EB	50.0	91	0	68	empty(to be used further)		L57#5	FG1165, FG1198	sequenzing starts ok, then mixture?, ok
192	O58-3	O58		07-26	14:50	Jan, Max H	Growth	235C1	Qiagen miniprep kit	Plasmid	58	58	pSBX1C3-BBa_I13507	EB	50.0	136	47.8	69			L58#3
193	O59-3	O59		07-26	14:50	Jan, Max H	Growth	236C1	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	EB	50.0	132	32.6	70	to be used further		L59#5	FG1167, FG1177, FG1199	frameshift, two fucking huge insertions, ok,longest,
194	O61	O61		07-26	14:50	Jan, Max H	Growth	238C1	Qiagen miniprep kit	Plasmid	61	61	pSBX4A5-BBa_I13507	EB	50.0	108	0	71	to be used further		L61#3	FG1169	starts ok then mixture
195	O62	O62		07-26	14:50	Jan, Max H	Growth	239C1	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	EB	50.0	570	37.3	72	to be used further		L62#3	FG1170	ok
196	O63	O63		07-26	14:50	Jan, Max H	Growth	240C1	Qiagen miniprep kit	Plasmid	63	63	pSBX4K5-BBa_I13507	EB	50.0	101	47.8	73	discarded		L63#3	FG1187	bad sequencing
197	O57-3	O57		07-26	14:50	Jan, Max H	Growth	234C2	Qiagen miniprep kit	Plasmid	57	57	pSBX1A3-BBa_I13507	EB	50.0	83	47.8	74	discard		L57#5	FG1173	bad sequencing
198	O58-4	O58		07-26	14:50	Jan, Max H	Growth	235C2	Qiagen miniprep kit	Plasmid	58	58	pSBX1C3-BBa_I13507	EB	50.0	49	46.8	75			L58#3	FG1175	ok
199	O59-4	O59		07-26	14:50	Jan, Max H	Growth	236C2	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	EB	50.0	112	25.5	76			L59#5	FG1178	ok
200	O61-2	O61		07-26	14:50	Jan, Max H	Growth	238C2	Qiagen miniprep kit	Plasmid	61	61	pSBX4A5-BBa_I13507	EB	50.0	47	0	77	discarded		L61#3	FG1181	bad sequencing
201	O62-2	O62		07-26	14:50	Jan, Max H	Growth	239C2	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	EB	50.0	326	47.8	78			L62#3
202	O63-2	O63		07-26	14:50	Jan, Max H	Growth	240C2	Qiagen miniprep kit	Plasmid	63	63	pSBX4K5-BBa_I13507	EB	50.0	55	0	79			L63#3	FG1171	no sequenzing
203	O57-4	O57		07-26	14:50	Jan, Max H	Growth	234C3	Qiagen miniprep kit	Plasmid	57	57	pSBX1A3-BBa_I13507	EB	50.0	76	47.8	80	discard		L57#5	FG1174	bad sequencing
204	O58-5	O58		07-26	14:50	Jan, Max H	Growth	235C3	Qiagen miniprep kit	Plasmid	58	58	pSBX1C3-BBa_I13507	EB	50.0	34	0	81	to be used further		L58#3	FG1166	bad sequenzing
205	O59-5	O59		07-26	14:50	Jan, Max H	Growth	236C3	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	EB	50.0	133	47.8	82			L59#5	FG1179	ok, short read
206	O61-3	O61		07-26	14:50	Jan, Max H	Growth	238C3	Qiagen miniprep kit	Plasmid	61	61	pSBX4A5-BBa_I13507	EB	50.0	68	0	83	discarded		L61#3	FG1182	bad sequencing
207	O62-3	O62		07-26	14:50	Jan, Max H	Growth	239C3	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	EB	50.0	214	43.1	84			L62#3
208	O63-3	O63		07-26	14:50	Jan, Max H	Growth	240C3	Qiagen miniprep kit	Plasmid	63	63	pSBX4K5-BBa_I13507	EB	50.0	40	47.8	85	to be used further		L63#3	FG1188	bad sequencing
209	O65-2	O65	s/Csi2jWhAjfYrFvcWQ	06-01	15:30				Distribution	Plasmid	65	65	pSB1C3-BBa_J04450			0	0.0
210	O65-3	O65	s/q6QmqRNBd4AcT6Z4j	06-01	15:30				Distribution	Plasmid	65	65	pSB1C3-BBa_J04450			0	0.0
211	O65-4	O65	s/bYEeipaiNwsmYDJG3	06-01	15:30				Distribution	Plasmid	65	65	pSB1C3-BBa_J04450			0	0.0
212	O65-5	O65	s/Dbq95DeScbMpSi5EW	06-01	15:30				Distribution	Plasmid	65	65	pSB1C3-BBa_J04450			0	0.0
213	O65-6	O65	s/Kmjb927eeqrLKE68G	06-01	15:30				Distribution	Plasmid	65	65	pSB1C3-BBa_J04450			0	0.0
214	R94	R94		07-27	11:40	Anna, Jakob	Gel	43-2-1	Gel extraction	Restriction	94		pSB1T3-BBa_J04450 (E/X)	EB	30.0	8	30.0		8.316666667
215	R94-2	R94		07-27	11:40	Anna, Jakob	Gel	43-3-1	Gel extraction	Restriction	94		pSB1T3-BBa_J04450 (E/X)	EB	30.0	8	14.3		249.5
216	R95	R95		07-27	11:40	Anna, Jakob	Gel	43-4-1	Gel extraction	Restriction	95		pSB1T3-BBa_J04450 (E/P)	EB	30.0	6	30.0		5.766666667
217	R95-2	R95		07-27	11:40	Anna, Jakob	Gel	43-5-1	Gel extraction	Restriction	95		pSB1T3-BBa_J04450 (E/P)	EB	30.0	6	25.2
218	R96	R96		07-27	11:40	Anna, Jakob	Gel	43-6-2	Gel extraction	Restriction	96		pSB1A2-BBa_I13507 (X/P)	EB	30.0	3	30.0		2.5
219	R96-2	R96		07-27	11:40	Anna, Jakob	Gel	43-7-2	Gel extraction	Restriction	96		pSB1A2-BBa_I13507 (X/P)	EB	30.0	3	18.0
220	R97	R97		07-27	11:40	Anna, Jakob	Gel	43-8-1	Gel extraction	Restriction	97		pSB3T5-BBa_J04450 (E/X)	EB	30.0	8	30.0		8.283333333
221	R97-2	R97		07-27	11:40	Anna, Jakob	Gel	43-9-1	Gel extraction	Restriction	97		pSB3T5-BBa_J04450 (E/X)	EB	30.0	8	30.0
222	R61-3	R61		07-28	0:30	Jakob	Purification	R61	Dilution	Restriction	61		T7prom	H2O	100.0	9	98.5
223	O60	O60		07-28	0:30	Jan	Growth	242C7	Qiagen miniprep kit	Plasmid	60	60	pSBX1T3-BBa_I13507	EB	50.0	79	24.5				L60#3	FG1168	ok
224	O60-2	O60		07-28	0:30	Jan	Growth	242C8	Qiagen miniprep kit	Plasmid	60	60	pSBX1T3-BBa_I13507	EB	50.0	140	35.7				L60#3
225	L0	L0	s/pMavcx63HXFvya3st	07-25	12:00	MaxW	Extern	?	ask Holger	Lambda DNA	0		?	storage buffer	100.0	1	83.0
226	O30-2	O30		07-28	23:00	Constantin	Purification	O30	Dilution	Plasmid	30	30	pVS07	H2O	151.0	1	140.0
227	O29-2	O29		07-28	23:00	Constantin	Purification	O29	Dilution	Plasmid	29	29	pVS41	H2O	114.0	1	104.0
228	R98	R98		07-28	23:30	Jakob	Gel	44-2-1,44-3-1	Gel extraction	Restriction	98		pSBX1A3-BBa_I13507 (E/S)	EB	30.0	22	19.8
229	R99	R99		07-28	23:30	Jakob	Gel	44-4-1,44-5-1	Gel extraction	Restriction	99		pSBX1C3-BBa_I13507 (E/S)	EB	30.0	13	6.2
230	R100	R100		07-28	23:30	Jakob	Gel	44-6-1,44-7-1	Gel extraction	Restriction	100		pSBX1K3-BBa_I13507 (E/S)	EB	30.0	19	14.2
231	R101	R101		07-28	23:30	Jakob	Gel	44-8-1,44-9-1	Gel extraction	Restriction	101		pSBX1T3-BBa_I13507 (E/S)	EB	30.0	8	-1.8
232	R102	R102		07-28	23:30	Jakob	Gel	44-10-1,44-11-1	Gel extraction	Restriction	102		pSBX4A5-BBa_I13507 (E/S)	EB	30.0	15	16.5
233	R103	R103		07-28	23:30	Jakob	Gel	44-13-1,44-14-1	Gel extraction	Restriction	103		pSBX4C5-BBa_I13507 (E/S)	EB	30.0	18	14.2
234	R104	R104		07-28	23:30	Jakob	Gel	44-15-1,44-16-1	Gel extraction	Restriction	104		pSBX4K5-BBa_I13507 (E/S)	EB	30.0	7	4.9
235	R105	R105		07-28	23:30	Jakob	Gel	44-17-1,44-18-1	Gel extraction	Restriction	105		pSB1C3-2ZSspH6:GFP (E/S)	EB	30.0	13	23.9
236	R106	R106		07-28	23:30	Jakob	Gel	44-19-1,44-20-1	Gel extraction	Restriction	106		pSB1C3-2ZNpuH6:GFP (E/S)	EB	30.0	11	23.0
237	R107	R107		07-29	20:30	Charlotte	Gel	46-9-1	Gel extraction	Restriction	107		pSB1C3-2ZSspH6:GFP (E/S)	H2O	30.0	11	19.5
238	R107-2	R107		07-29	20:30	Charlotte	Gel	46-10-1	Gel extraction	Restriction	107		pSB1C3-2ZSspH6:GFP (E/S)	H2O	30.0	19	30.0
239	R108	R108		07-29	20:30	Charlotte	Gel	46-11-1	Gel extraction	Restriction	108		pSB1C3-2ZNpuH6:GFP (E/S)	H2O	30.0	9	19.3
240	R108-2	R108		07-29	20:30	Charlotte	Gel	46-12-1	Gel extraction	Restriction	108		pSB1C3-2ZNpuH6:GFP (E/S)	H2O	30.0	96	30.0
241	P46	P46		07-29		Anna	Gel	45-2	Gel extraction	PP	46		Lambda-Lys_pSBX_pSBX	H2O	30.0	16	30.0
242	P46-2	P46		07-29		Anna	Gel	45-3	Gel extraction	PP	46		Lambda-Lys_pSBX_pSBX	H2O	30.0	10	30.0
243	P47	P47		07-29		Anna	Gel	47-2	Gel extraction	PP	47		GFP_pSBX_pSBX	H2O	30.0	16	30.0
244	P47-2	P47		07-29		Anna	Gel	48-2	Gel extraction	PP	47		GFP_pSBX_pSBX	H2O	30.0	18	30.0
245	P48	P48		07-29		Anna	Gel	48-3	Gel extraction	PP	48		NpuDnaEN_	H2O	30.0	70	30.0
246	P48-2	P48		07-29		Anna	Gel	48-4	Gel extraction	PP	48		NpuDnaEN_	H2O	30.0	42	30.0
247	P49	P49		07-29		Anna	Gel	48-5	Gel extraction	PP	49		NpuDnaEC_	H2O	30.0	50	30.0
248	P49-2	P49		07-29		Anna	Gel	48-6	Gel extraction	PP	49		NpuDnaEC_	H2O	30.0	33	30.0
249	P44	P44		07-29		Anna	Gel	48-7	Gel extraction	PP	44		ccdB_BsmBI_BsmBI	H2O	30.0	45	30.0
250	P44-2	P44		07-29		Anna	Gel	48-8	Gel extraction	PP	44		ccdB_BsmBI_BsmBI	H2O	30.0	65	30.0
251	P45	P45		07-29		Anna	Gel	48-9	Gel extraction	PP	45		mRFP_BsaI_BsaI	H2O	30.0	37	30.0
252	P45-2	P45		07-29		Anna	Gel	48-10	Gel extraction	PP	45		mRFP_BsaI_BsaI	H2O	30.0	50	30.0
253	O80	O80		07-31	23:00	Charlotte	Growth	279C1	Qiagen miniprep kit	Plasmid	80	80	pSBX4C5-2ZSspH6:GFP	EB	30.0	0	15.0	87	discarded		S80
254	O80-2	O80		07-31	23:00	Charlotte	Growth	279C2	Qiagen miniprep kit	Plasmid	80	80	pSBX4C5-2ZSspH6:GFP	EB	30.0	307	23.5		discarded		S80
255	O81	O81		08-01	11:00	Charlotte	Growth	280C2	Qiagen miniprep kit	Plasmid	81	81	pSBX4C5-2ZNpuH6:GFP	EB	30.0	0	30.0				S81
256	O43-2	O43		08-01	11:00	Charlotte	Growth	164C1-1	Qiagen miniprep kit	Plasmid	43	43	pSB1C3-BBa_I746907	EB	30.0	0	0				D2014-3-12I
257	O77	O77		08-02	18:00	Caro	Growth	297C1	Qiagen miniprep kit	Plasmid	77	77	pSBX1C3-T7RBS_BBv2	H2O	30.0	586	30.0	89			S77
258	O78	O78		08-02	18:00	Caro	Growth	289C1	Qiagen miniprep kit	Plasmid	78	78	pSBX1K3-T7RBS_BBv2	H2O	30.0	219	9.8	90			S78	76DB35	seems ok; some bases badly called
259	O78-2	O78		08-02	18:00	Caro	Growth	289C2	Qiagen miniprep kit	Plasmid	78	78	pSBX1K3-T7RBS_BBv2	H2O	30.0	136	11.6	91			S78
260	O78-3	O78		08-02	18:00	Caro	Growth	289C3	Qiagen miniprep kit	Plasmid	78	78	pSBX1K3-T7RBS_BBv2	H2O	30.0	155	23.5	92			S78
261	O72	O72		08-02	18:00	Caro	Growth	285C1	Qiagen miniprep kit	Plasmid	72	72	pSBX4A5-T7RBS-IGEMHD_v3	H2O	30.0	184	30.0	93			S72#2	FG1191	good sequencing, ok
262	O72-2	O72		08-02	18:00	Caro	Growth	294C1	Qiagen miniprep kit	Plasmid	72	72	pSBX4A5-T7RBS-IGEMHD_v3	H2O	30.0	129	0	94	discarded		S72#2
263	O70	O70		08-02	18:00	Caro	Growth	292C1	Qiagen miniprep kit	Plasmid	70	70	pSBX1K3-T7RBS-IGEMHD_v3	H2O	30.0	105	30.0	95			S70#2
264	O70-2	O70		08-02	18:00	Caro	Growth	292C2	Qiagen miniprep kit	Plasmid	70	70	pSBX1K3-T7RBS-IGEMHD_v3	H2O	30.0	103	29.0	96			S70#2
265	O72-3	O72		08-02	20:00	Caro	Purification	O72	Dilution	Plasmid	72	72	pSBX4A5-T7RBS-IGEMHD_v3	H2O	184.0	1	0		discarded
266	O72-4	O72		08-02	20:00	Caro	Purification	O72-2	Dilution	Plasmid	72	72	pSBX4A5-T7RBS-IGEMHD_v3	H2O	129.0	1	0		discarded
267	O70-3	O70		08-02	20:00	Caro	Purification	O70	Dilution	Plasmid	70	70	pSBX1K3-T7RBS-IGEMHD_v3	H2O	105.0	1	104.0
268	O70-4	O70		08-02	20:00	Caro	Purification	O70-2	Dilution	Plasmid	70	70	pSBX1K3-T7RBS-IGEMHD_v3	H2O	103.0	1	92.0		sequenzed descendands
269	R111	R111		08-02	23:00	Jan	Gel	50-2-1,50-3-1	Gel extraction	Restriction	111		pSBX1T3-BBa_I13507 (E/S)	H2O	30.0	10	23.6		estimated concentration, used wrong columns
270	R112	R112		08-02	23:00	Jan	Gel	50-4-1,50-5-1	Gel extraction	Restriction	112		pSBX4C5-BBa_I13507 (E/S)	H2O	30.0	11	23.5		estimated concentration, used wrong columns
271	P61	P61		08-03	1:00	MaxW	Gel	52-6, 52-7	Gel extraction	PP	61		pSBX4A5_taataa_atg	H2O	30.0	5	30.0
272	P60	P60		08-03	14:00	Caro	Gel	53-2,53-3,53-4	Gel extraction	PP	60		pSBX4A5_mRFP-BsaI_BsmBI-ccdB	H2O	30.0	16	30.0
273	P59	P59		08-03	14:00	Caro	Gel	53-6	Gel extraction	PP	59		pSBX4A5_ccdB-BsmBI_BsaI-mRFP	H2O	30.0	63	30.0
274	P59-2	P59		08-03	14:00	Caro	Gel	53-7,53-8	Gel extraction	PP	59		pSBX4A5_ccdB-BsmBI_BsaI-mRFP	H2O	30.0	105	30.0
275	O58-6	O58		08-03	15:31	Anna	Growth	235C3-1	Qiagen miniprep kit	Plasmid	58	58	pSBX1C3-BBa_I13507	H2O	30.0	42	6.1				L58#3	FG1176	bad sequencing
276	O61-4	O61		08-03	15:31	Anna	Growth	238C1-1	Qiagen miniprep kit	Plasmid	61	61	pSBX4A5-BBa_I13507	H2O	30.0	109	0				L61#3	FG1183	ok
277	O63-4	O63		08-03	15:31	Anna	Growth	240C2-1	Qiagen miniprep kit	Plasmid	63	63	pSBX4K5-BBa_I13507	H2O	30.0	72	25.8				L63#3	FG1189	ok
278	O62-4	O62		08-03	15:31	Anna	Growth	239C1-1	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	H2O	30.0	112	30.0				L62#3
279	O60-3	O60		08-03	15:31	Anna	Growth	242C13	Qiagen miniprep kit	Plasmid	60	60	pSBX1T3-BBa_I13507	H2O	30.0	80	4.0	98			L60#3	FG1180	ok
280	R113	R113		08-04	2:00	Jakob	Gel	56-2-1, 56-3-1	Gel extraction	Restriction	113		pSB1AK8-BBa_I712074 (S/P)	H2O	30.0	16	24.7
281	R114	R114		08-04	2:00	Jakob	Gel	56-4-2, 56-5-2	Gel extraction	Restriction	114		pSB1C3-2ZSspH6:GFP (X/P)	H2O	30.0	15	25.9
282	R115	R115		08-04	2:00	Jakob	Gel	56-6-2, 56-7-2	Gel extraction	Restriction	115		pSB1C3-2ZNpuH6:GFP (X/P)	H2O	30.0	16	26.0
283	O62-5	O62		08-04	17:22	Anna	Growth	327C1	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	H2O	50.0		50.0	99			O62
284	O62-6	O62		08-04	17:22	Anna	Growth	327C2	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	H2O	50.0	53	31.0	100	2 Tage lang induziert -> Fehler?		O62	FG1184	ok, longest
285	O62-7	O62		08-04	17:22	Anna	Growth	327C3	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	H2O	50.0		50.0	101			O62
286	O62-8	O62		08-04	17:22	Anna	Growth	327C4	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	H2O	50.0	0	50.0	102			O62
287	O62-9	O62		08-04	17:22	Anna	Growth	327C5	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	H2O	50.0	20	50.0	103	2 Tage lang induziert -> Fehler?		O62	FG1185	ok
288	O62-10	O62		08-04	17:22	Anna	Growth	327C6	Qiagen miniprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	H2O	50.0	72	22.2	104	2 Tage lang induziert -> Fehler?		O62	FG1186	ok
289	O43-3	O43		08-05	9:00	Charlotte	Growth	164C1-2	Qiagen miniprep kit	Plasmid	43	43	pSB1C3-BBa_I746907	H2O	50.0	0	50.0				D2014-3-12I
290	O44-7	O44		08-05	9:00	Lisa	Growth	347C1	Qiagen miniprep kit	Plasmid	44	44	pSB1C3-2ZSspH6:GFP	H2O	30.0	0	30.0				O44-4
291	O44-8	O44		08-05	9:00	Lisa	Growth	347C2	Qiagen miniprep kit	Plasmid	44	44	pSB1C3-2ZSspH6:GFP	H2O	30.0	0	30.0	110			O44-4
292	O45-7	O45		08-05	9:00	Lisa	Growth	348C1	Qiagen miniprep kit	Plasmid	45	45	pSB1C3-2ZNpuH6:GFP	H2O	30.0	0	30.0				O45-5
293	O45-8	O45		08-05	9:00	Lisa	Growth	348C2	Qiagen miniprep kit	Plasmid	45	45	pSB1C3-2ZNpuH6:GFP	H2O	30.0	0	30.0	108			O45-5
294	O36-3	O36		08-05	9:00	Lisa	Growth	349C1	Qiagen miniprep kit	Plasmid	36	36	pSB1AK8-BBa_I712074	H2O	30.0	121	21.7	109			O36
295	O36-4	O36		08-05	9:00	Lisa	Growth	349C2	Qiagen miniprep kit	Plasmid	36	36	pSB1AK8-BBa_I712074	H2O	30.0	123	12.1				O36
296	O71	O71		08-05	9:00	Anna	Growth	328C2	Qiagen miniprep kit	Plasmid	71	71	pSBX1T3-T7RBS-IGEMHD_v3	H2O	50.0	32	50.0		Sind 2 Tage lang gewachsen -> Fehler?		S71#3
297	O71-2	O71		08-05	9:00	Anna	Growth	328C3	Qiagen miniprep kit	Plasmid	71	71	pSBX1T3-T7RBS-IGEMHD_v3	H2O	50.0	57	32.5		Sind 2 Tage lang gewachsen -> Fehler?		S71#3	FG1190	good sequencing, ok
298	O73	O73		08-05	9:00	Anna	Growth	329C2	Qiagen miniprep kit	Plasmid	73	73	pSBX4C5-T7RBS-IGEMHD_v3	H2O	50.0	437	50.0		Sind 2 Tage lang gewachsen -> Fehler?		S73#3	FG1192	good sequencing, ok
299	O73-2	O73		08-05	9:00	Anna	Growth	329C3	Qiagen miniprep kit	Plasmid	73	73	pSBX4C5-T7RBS-IGEMHD_v3	H2O	50.0	411	45.1		Sind 2 Tage lang gewachsen -> Fehler?		S73#3
300	O71-3	O71		08-05	13:41	MaxW	Purification	O71-2	Dilution	Plasmid	71	71	pSBX1T3-T7RBS-IGEMHD_v3	H2O	57.0	1	45.0
301	O73-3	O73		08-05	13:41	MaxW	Purification	O73	Dilution	Plasmid	73	73	pSBX4C5-T7RBS-IGEMHD_v3	H2O	437.0	1	0		discarded
302	P61-2	P61		08-05	17:37	MaxW	Gel	65-2	Gel extraction	PP	61		pSBX4A5_taataa_atg	H2O	30.0	20	30.0
303	P73	P73		08-05	17:37	MaxW	Gel	65-3	Gel extraction	PP	73		pSBX1T3_taataa_atg	H2O	30.0	13	30.0
304	P74	P74		08-05	17:37	MaxW	Gel	65-4	Gel extraction	PP	74		pSBX4C5_taataa_atg	H2O	30.0	15	30.0
305	P69	P69		08-05	20:00	Jakob	Gel	66-2, 66-3, 66-4	Gel extraction	PP	69		pSBX1T3_ccdB-BsmBI_BsaI-mRFP	H2O	30.0	15	30.0
306	P70	P70		08-05	20:00	Jakob	Gel	66-5, 66-6, 66-7	Gel extraction	PP	70		pSBX1T3_mRFP-BsaI_BsmBI-ccdB	H2O	30.0	15	30.0
307	P71	P71		08-05	20:00	Jakob	Gel	66-8, 66-9, 66-10	Gel extraction	PP	71		pSBX4C5_ccdB-BsmBI_BsaI-mRFP_N	H2O	30.0	15	30.0
308	P72	P72		08-05	20:00	Jakob	Gel	66-11, 66-12, 66-13	Gel extraction	PP	72		pSBX4C5_mRFP-BsaI_BsmBI-ccdB_C	H2O	30.0	15	30.0
309	P75	P75		08-06	01:28	MaxW	Gel	67-2, 67-3	Gel extraction	PP	75		NpuDnaEC_T7_RBS-BsaI_mRFP	H2O		15	0.0
310	P76	P76		08-06	01:28	MaxW	Gel	67-4, 67-5	Gel extraction	PP	76		NpuDnaEN_mRFP_BsaI-taataa_iGEMHD	H2O	30.0	50	30.0
311	O65-7	O65		08-06	12:45	Caro	Growth	366C1	Qiagen miniprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	30.0	86	21.3				D2014-4-4B	FG1193	ok
312	O65-8	O65		08-06	12:45	Caro	Growth	366C2	Qiagen miniprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	30.0	71	30.0				D2014-4-4B
313	O65-9	O65		08-06	12:45	Caro	Growth	366C3	Qiagen miniprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	30.0	122	30.0				D2014-4-4B
314	O88	O88		08-06	12:45	Caro	Growth	367C1	Qiagen miniprep kit	Plasmid	88	88	pSB1C3-BBa_J52008	H2O	30.0	70	30.0				D2014-3-3J	FG1194	ok
315	O88-2	O88		08-06	12:45	Caro	Growth	367C2	Qiagen miniprep kit	Plasmid	88	88	pSB1C3-BBa_J52008	H2O	30.0	97	30.0				D2014-3-3J
316	O88-3	O88		08-06	12:45	Caro	Growth	367C3	Qiagen miniprep kit	Plasmid	88	88	pSB1C3-BBa_J52008	H2O	30.0	90	30.0				D2014-3-3J
317	O89	O89		08-06	12:45	Caro	Growth	368C1	Qiagen miniprep kit	Plasmid	89	89	pSB1AK8-BBa_I712019	H2O	30.0	175	30.0				D2014-4-20C	FG1195	ok, good
318	O89-2	O89		08-06	12:45	Caro	Growth	368C2	Qiagen miniprep kit	Plasmid	89	89	pSB1AK8-BBa_I712019	H2O	30.0	208	30.0				D2014-4-20C
319	O89-3	O89		08-06	12:45	Caro	Growth	368C3	Qiagen miniprep kit	Plasmid	89	89	pSB1AK8-BBa_I712019	H2O	30.0	159	30.0				D2014-4-20C
320	O90	O90		08-06	12:45	Caro	Growth	369C1	Qiagen miniprep kit	Plasmid	90	90	pSB1C3-BBa_J06702	H2O	30.0	77	30.0				D2014-3-15B	FG1196	ok, good
321	O90-2	O90		08-06	12:45	Caro	Growth	369C2	Qiagen miniprep kit	Plasmid	90	90	pSB1C3-BBa_J06702	H2O	30.0	82	0		discarded		D2014-3-15B
322	O90-3	O90		08-06	12:45	Caro	Growth	369C3	Qiagen miniprep kit	Plasmid	90	90	pSB1C3-BBa_J06702	H2O	30.0	65	0		discarded		D2014-3-15B
323	O91	O91		08-06	12:45	Caro	Growth	370C1	Qiagen miniprep kit	Plasmid	91	91	pSB1A2-BBa_E0040	H2O	30.0	120	30.0				D2014-4-13L	FG1197	ok, good
324	O91-2	O91		08-06	12:45	Caro	Growth	370C2	Qiagen miniprep kit	Plasmid	91	91	pSB1A2-BBa_E0040	H2O	30.0	86	0		discarded		D2014-4-13L
325	O91-3	O91		08-06	12:45	Caro	Growth	370C3	Qiagen miniprep kit	Plasmid	91	91	pSB1A2-BBa_E0040	H2O	30.0	158	0		discarded		D2014-4-13L
326	O60-4	O60		08-06	22:00	Anna	Growth	325C6	Qiagen miniprep kit	Plasmid	60	60	pSBX1T3-BBa_I13507	H2O	50.0	0	50.0		Bin mir nicht ganz sicher, was drin ist. Besser nicht verwenden.		O60
327	O60-5	O60		08-06	22:00	Anna	Growth	326C1	Qiagen miniprep kit	Plasmid	60	60	pSBX1T3-BBa_I13507	H2O	100.0	0	100.0		Bin mir nicht ganz sicher, was drin ist. Besser nicht verwenden.		O60
328	O59-6	O59		08-08	11:51	Anna	Growth	384C1	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O	50.0	20	50.0	133			O59-3
329	O59-7	O59		08-08	11:51	Anna	Growth	384C2	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O	50.0	26	50.0	134			O59-3
330	O59-8	O59		08-08	11:51	Anna	Growth	384C3	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O	50.0	14	50.0	135			O59-3
331	O59-9	O59		08-08	11:51	Anna	Growth	385C1	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O	50.0	17	50.0	136			O59-5
332	O59-10	O59		08-08	11:51	Anna	Growth	385C2	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O	50.0	16	50.0	137			O59-5
333	O59-11	O59		08-08	11:51	Anna	Growth	385C3	Qiagen miniprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O	50.0	0	50.0	138			O59-5
334	O61-5	O61		08-08	11:51	Anna	Growth	386C2	Qiagen miniprep kit	Plasmid	61	61	pSBX4A5-BBa_I13507	H2O	50.0	17	29.0	140			O61-4
335	O61-6	O61		08-08	11:51	Anna	Growth	386C3	Qiagen miniprep kit	Plasmid	61	61	pSBX4A5-BBa_I13507	H2O	50.0	24	8.8	141			O61-4
336	O63-5	O63		08-08	11:51	Anna	Growth	387C1	Qiagen miniprep kit	Plasmid	63	63	pSBX4K5-BBa_I13507	H2O	50.0	10	0	142	discarded		O63-4
337	O63-6	O63		08-08	11:51	Anna	Growth	387C3	Qiagen miniprep kit	Plasmid	63	63	pSBX4K5-BBa_I13507	H2O	50.0	27	0	144	discarded		O63-4
340	O71-5	O71		08-08	14:30	Jakob	Purification	O71-2	Dilution	Plasmid	71	71	pSBX1T3-T7RBS-IGEMHD_v3	H2O	57.20	1	0		discarded
341	O72-5	O72		08-08	14:30	Jakob	Purification	O72	Dilution	Plasmid	72	72	pSBX4A5-T7RBS-IGEMHD_v3	H2O	184.10	1	180.10
342	O73-5	O73		08-08	14:30	Jakob	Purification	O73	Dilution	Plasmid	73	73	pSBX4C5-T7RBS-IGEMHD_v3	H2O	436.60	1	420.60
343	O65-10	O65		08-08	14:30	Jakob	Purification	O65-7	Dilution	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	86.00	1	74.00
344	O88-4	O88		08-08	14:30	Jakob	Purification	O88	Dilution	Plasmid	88	88	pSB1C3-BBa_J52008	H2O	70.00	1	68.00
345	O89-4	O89		08-08	14:30	Jakob	Purification	O89	Dilution	Plasmid	89	89	pSB1AK8-BBa_I712019	H2O	175.00	1	173.00
346	O90-4	O90		08-08	14:30	Jakob	Purification	O90	Dilution	Plasmid	90	90	pSB1C3-BBa_J06702	H2O	77.00	1	71.00
347	O91-4	O91		08-08	14:30	Jakob	Purification	O91	Dilution	Plasmid	91	91	pSB1A2-BBa_E0040	H2O	120.00	1	110.00
348	P46-3	P46		08-08	17:45	Charlotte	Gel	74-2, 74-3	Gel extraction	PP	46		Lambda-Lys_pSBX_pSBX	H2O	30.0	5	30.0		43 echte konz.
349	P75-2	P75		08-08	17:45	Charlotte	Gel	74-4, 74-5	Gel extraction	PP	75		NpuDnaEC_T7_RBS-BsaI_mRFP	H2O	30.0	15	28.0		63.0
350	P76-2	P76		08-08	17:45	Charlotte	Gel	74-6, 74-7	Gel extraction	PP	76		NpuDnaEN_mRFP_BsaI-taataa_iGEMHD	H2O	30.0	80	30.0		139.5
351	P61-3	P61		08-08	17:45	Charlotte	Gel	74-8, 74-9	Gel extraction	PP	61		pSBX4A5_taataa_atg	H2O	30.0	58	30.0		57.5
352	P61-4	P61		08-08	17:45	Charlotte	Gel	74-8, 74-9	Gel extraction	PP	61		pSBX4A5_taataa_atg	H2O	30.0	5	30.0		20.0
353	P73-2	P73		08-08	17:45	Charlotte	Gel	74-10, 74-11	Gel extraction	PP	73		pSBX1T3_taataa_atg	H2O	30.0	30	30.0		30.0
354	P74-2	P74		08-08	17:45	Charlotte	Gel	74-12, 74-13	Gel extraction	PP	74		pSBX4C5_taataa_atg	H2O	30.0	27	30.0		27.0
355	O58-7	O58		08-08	17:45	Anna	Growth	383C3	Qiagen miniprep kit	Plasmid	58	58	pSBX1C3-BBa_I13507	H2O		0	0.0				O58-4
356	P44-3	P44		08-08	19:00	Jakob	Gel	82-2-1, 82-3-1	Gel extraction	PP	44		ccdB_BsmBI_BsmBI	H2O	30.0	109	30.0
357	P45-3	P45		08-09	20:00	Jakob	Gel	82-4-1, 82-5-1	Gel extraction	PP	45		mRFP_BsaI_BsaI	H2O	30.0	24	30.0
358	P48-3	P48		08-10	21:00	Jakob	Gel	82-6-1, 82-7-1	Gel extraction	PP	48		NpuDnaEN_	H2O	30.0	91	30.0
359	P49-3	P49		08-11	22:00	Jakob	Gel	82-8-1, 82-9-1	Gel extraction	PP	49		NpuDnaEC_	H2O	30.0	62	30.0
360	P45-4	P45		08-09	1:30	Jakob	Gel	83-3-1, 83-4-1	Gel extraction	PP	45		mRFP_BsaI_BsaI	H2O	30.0	55	30.0
361	P69-2	P69		08-09	3:50	Jakob	Gel	84-2-1, 84-3-1	Gel extraction	PP	69		pSBX1T3_ccdB-BsmBI_BsaI-mRFP	H2O	30.0	41	30.0
362	P70-2	P70		08-09	3:50	Jakob	Gel	84-4-1, 84-5-1	Gel extraction	PP	70		pSBX1T3_mRFP-BsaI_BsmBI-ccdB	H2O	30.0	35	30.0
363	P59-3	P59		08-09	3:50	Jakob	Gel	84-6-1, 84-7-1	Gel extraction	PP	59		pSBX4A5_ccdB-BsmBI_BsaI-mRFP	H2O	30.0	19	30.0
364	P60-2	P60		08-09	3:50	Jakob	Gel	84-8-1, 84-9-1	Gel extraction	PP	60		pSBX4A5_mRFP-BsaI_BsmBI-ccdB	H2O	30.0	21	30.0
365	P71-2	P71		08-09	3:50	Jakob	Gel	84-10-1, 84-11-1	Gel extraction	PP	71		pSBX4C5_ccdB-BsmBI_BsaI-mRFP_N	H2O	30.0	30	30.0
366	P72-2	P72		08-09	3:50	Jakob	Gel	84-12-1, 84-13-1	Gel extraction	PP	72		pSBX4C5_mRFP-BsaI_BsmBI-ccdB_C	H2O	30.0	15	30.0
367	O90-5	O90		08-09	03:54	Jan	Purification	O90	Dilution	Plasmid	90	90	pSB1C3-BBa_J06702	H2O	77.0	1	55.0
368	P97	P97		08-09	22:44	Caro	Gel	76-1-2	Gel extraction	PP	97		mRFPn_1-154	H2O	30.0	138	30.0
369	P98	P98		08-09	22:44	Caro	Gel	76-3-4	Gel extraction	PP	98		mRFP_1-168	H2O	30.0	164	30.0
370	P99	P99		08-09	22:44	Caro	Gel	76-6-7	Gel extraction	PP	99		mRFPc_155-225	H2O	30.0	124	30.0
371	P100	P100		08-09	22:44	Caro	Gel	76-8, 77-1	Gel extraction	PP	100		mRFPc_169-225	H2O	30.0	162	30.0
372	P101	P101		08-09	22:44	Caro	Gel	77-3-4	Gel extraction	PP	101		RenLucN_1-229_H6_GATG_BsaI_KGG_TGCT_BsaI	H2O	30.0	158	30.0
373	P102	P102		08-09	22:44	Caro	Gel	77-5-6	Gel extraction	PP	102		RenLucC_230-311_NWC_CAAC_BsaI_H6_TAAT_BsaI	H2O	30.0	173	30.0
374	P103	P103		08-09	22:44	Caro	Gel	77-7-8	Gel extraction	PP	103		FireLucN_1-437_H6_GATG_BsaI_KGG_TGCT_BsaI	H2O	30.0	99	30.0
375	P104	P104		08-09	22:44	Caro	Gel	78-1-2	Gel extraction	PP	104		FireLucC_438-550_NWC_CAAC_BsaI_H6_TAAT_BsaI	H2O	30.0	153	30.0
376	P105	P105		08-09	22:44	Caro	Gel	78-3-5	Gel extraction	PP	105		mCherryN_1-168_H6_GATG_BsaI_KGG_TGCT_BsaI	H2O	30.0	51	30.0
377	P106	P106		08-09	22:44	Caro	Gel	78-6-7	Gel extraction	PP	106		mCherryC_169-236_NWC_CAAC_BsaI_H6_TAAT_BsaI	H2O	30.0	126	30.0
378	P107	P107		08-09	22:44	Caro	Gel	78-8, 79-1	Gel extraction	PP	107		GFPn_1-64_H6_BsaI	H2O	30.0	104	30.0
379	P108	P108		08-09	22:44	Caro	Gel	79-2-3	Gel extraction	PP	108		GFPn_1-157_H6_BsaI	H2O	30.0	115	30.0
380	P109	P109		08-09	22:44	Caro	Gel	79-5-6	Gel extraction	PP	109		GFPc_65-238_NWC_CAAC_BsaI_H6_TAAT_BsaI	H2O	30.0	161	30.0
381	P110	P110		today	22:44	Caro	Gel	79-7-8	Gel extraction	PP	110		GFPc_158-238_NWC_CAAC_BsaI_H6_TAAT_BsaI	H2O	30.0	103	30.0
382	P79	P79		08-09	11:15	Jakob	Gel	90-2-1, 90-3-1	Gel extraction	PP	79		mRFP-NpuDnaEN-ccdB_BsaI_BsmBI	H2O	30.0	26	-11.6
383	P81	P81		08-09	12:15	Jakob	Gel	90-4-1, 90-5-1	Gel extraction	PP	81		ccdB-NpuDnaEC-mRFP_BsmBI_BsaI	H2O	30.0	47	-2.5
384	P112	P112	s/cmQMwHQNwJDdmZQFy	08-07	12:00	Jakob	Extern		from Dominik Niopek	PP	112		NEBGibsonPositiveControl				0.0
385	P59-4	P59		08-10	11:00	Caro	Gel	94-2-1, 94-3-1	Gel extraction	PP	59		pSBX4A5_ccdB-BsmBI_BsaI-mRFP	H2O	30.0	30	-3.3
386	P60-3	P60		08-10	11:00	Caro	Gel	94-4-1, 94-5-1	Gel extraction	PP	60		pSBX4A5_mRFP-BsaI_BsmBI-ccdB	H2O	30.0	30	-3.3
387	O80-3	O80		today	18:00	Constantin	Growth	300C4	Qiagen miniprep kit	Plasmid	80	80	pSBX4C5-2ZSspH6:GFP	H2O	30.0	0	30.0	148			O80-2	FG1203, FG1208	ok, ok
388	O81-2	O81		today	18:00	Constantin	Growth	301C3	Qiagen miniprep kit	Plasmid	81	81	pSBX4C5-2ZNpuH6:GFP	H2O	30.0	311	30.0	149			O81	FG1204, FG1209	pSBX4C5-2ZSspH6:GFP, pSBX4C5-2ZSspH6:GFP
389	O86	O86		today	18:00	Constantin	Growth	372C4	Qiagen miniprep kit	Plasmid	86	86	pSB1AK8-BBa_I712074-2ZSspH6:GFP	H2O	30.0	112	30.0	150	discarded		S86	FG1205, FG1210	is just pSB1AK8-BBa_I712074, is just pSB1AK8-BBa_I712074
390	O87	O87		today	18:00	Constantin	Growth	351C7	Qiagen miniprep kit	Plasmid	87	87	pSB1AK8-BBa_I712074-2ZNpuH6:GFP	H2O	30.0	180	30.0	151	discarded		S87	FG1206, FG1211	mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown, mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown
391	O48-2	O48		today	18:00	Constantin	Growth	263C1-4	Qiagen miniprep kit	Plasmid	48	48	pSB1AK8-T7-2ZNpu:(GFP-H6)	H2O	30.0	96	30.0				O48	FG1207, FG1212	ok, ok
392	P62	P62		08-10	22:00	Constantin	Gel	99-2-1	Gel extraction	PP	62		pSBX1K3_taataa_atg	H2O	30.0	33	30.0
399	P79-2	P79		08-11	11:40	Jakob	Gel	107-2-1, 107-3-1	Gel extraction	PP	79		mRFP-NpuDnaEN-ccdB_BsaI_BsmBI	H2O	30.0	8	28.7
400	P81-2	P81		08-11	11:40	Jakob	Gel	107-4-1, 107-5-1	Gel extraction	PP	81		ccdB-NpuDnaEC-mRFP_BsmBI_BsaI	H2O	30.0	22	29.5
401	P46-4	P46		08-11	3:00	Charlotte	Gel	103-2, 103-3	Gel extraction	PP	46		Lambda-Lys_pSBX_pSBX	H2O	30.0	8	30.0		estimated
402	O102	O102		08-11	17:30	Jan	Gel	105-3-1	Gel extraction	Plasmid	102	102	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	H2O	10.0		10.0
403	O102-2	O102		08-11	17:34	Jan	Assembly	A102	PCR purification kit	Plasmid	102	102	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	H2O	10.0		0
404	P79-3	P79		08-11	11:00	Jakob	Purification	P79-2	Dilution	PP	79		mRFP-NpuDnaEN-ccdB_BsaI_BsmBI	H2O	100.0	0	100.0
405	P81-3	P81		08-11	08-11	Jakob	Purification	P81-2	Dilution	PP	81		ccdB-NpuDnaEC-mRFP_BsmBI_BsaI	H2O	100.0	0	100.0
406	P122	P122		08-12	1:30	MaxW	Gel	110-2, 110-3	Gel extraction	PP	122		2ZNpu_pSBX_taataa_pSBX	H2O	30.0	125	30.0
407	O65-11	O65		08-12	13:00	Charlotte	Purification	O65-7	Dilution	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	86.0	1	23.8
408	P45-5	P45		08-12	17:50	Jakob	Gel	112-4-1	Gel extraction	PP	45		mRFP_BsaI_BsaI	H2O	30.0	93	30.0
409	O98	O98		08-12	21:43	MaxW	Assembly	A98#2	PCR purification kit	Plasmid	98	98	pSBX4C5-2Zv2Npu	H2O			0.0		discarded
410	O98-2	O98		08-12	21:43	MaxW	Assembly	A98#3	PCR purification kit	Plasmid	98	98	pSBX4C5-2Zv2Npu	H2O			0.0		discarded
411	O102-3	O102		08-13	3:10	MaxW	Growth	423C2	Qiagen miniprep kit	Plasmid	102	102	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	H2O	50.0	76	50.0	152			O102-2	FG1200, II2274	ok, ok
412	O102-4	O102		08-13	3:10	MaxW	Growth	423C3	Qiagen miniprep kit	Plasmid	102	102	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	H2O	50.0	70	50.0	153			O102-2	FG1201, II2275	ok, ok
413	O102-5	O102		08-13	3:10	MaxW	Growth	423C4	Qiagen miniprep kit	Plasmid	102	102	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	H2O	50.0	61	50.0	154	discarded		O102-2
414	O102-6	O102		08-13	3:10	MaxW	Growth	424C1	Qiagen miniprep kit	Plasmid	102	102	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	H2O	50.0	66	50.0	155			A102
415	O102-7	O102		08-13	3:10	MaxW	Growth	424C2	Qiagen miniprep kit	Plasmid	102	102	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	H2O	50.0	82	43.9	156			A102	FG1202, II2276	ok, ok
416	O103	O103		08-14	17:44	MaxW	Growth	433C1	Qiagen miniprep kit	Plasmid	103	103	pSBX1K3-2Zv2Npu	H2O	30.0	66	2.6	157	charlies zeug........		A103	FG1213, FG1214	wrong, charlie's stuff, wrong, charlie's stuff
417	O104	O104		08-14	17:44	MaxW	Growth	434C1	Qiagen miniprep kit	Plasmid	104	104	pSB1C3-BBa_I746908	H2O	30.0	98	30.0	158			D2014-4-5C	II2277, II2278	ok, ok
418	O13-3	O13		08-14	21:30	Jakob	Purification	O13	Dilution	Plasmid	13	13	pSB1C3-pKH6insert	H2O	102.0	1	98.0
419	O29-3	O29		08-14	21:30	Jakob	Purification	O29	Dilution	Plasmid	29	29	pVS41	H2O	114.0	1	98.0
420	O30-3	O30		08-14	21:30	Jakob	Purification	O30	Dilution	Plasmid	30	30	pVS07	H2O	151.6	1	145.6
421	P134	P134		08-14	22:00	MaxW	Gel	121-2, 121-3	Gel extraction	PP	134		LambdaLys_BsaI_c-ext_HISTAG_n-ext_BsaI	H2O	40.0	63	40.0
422	P135	P135		08-14	22:00	MaxW	Gel	121-4, 121-5	Gel extraction	PP	135		LambdaLys_BsaI_c-ext_RIGIDLINKER_n-ext_BsaI	H2O	40.0	64	40.0
423	O103-2	O103		08-14	22:26	MaxW	Growth	433C4	Qiagen miniprep kit	Plasmid	103	103	pSBX1K3-2Zv2Npu	H2O	30.0	93	17.8	159	restriktion sagt: passt		A103	II2279, II2280	ok (silent mutation in selection marker), ok (silent mutation in selection marker)
424	P44-4	P44		08-15	O98	Jakob	Gel	122-2-1, 122-3-1	Gel extraction	PP	44		ccdB_BsmBI_BsmBI	H2O	30.0	121	30.0
425	P45-6	P45		08-15	08-15	Jakob	Gel	122-4-1, 122-5-1	Gel extraction	PP	45		mRFP_BsaI_BsaI	H2O	30.0	70	30.0
426	P48-4	P48		08-15	08-15	Jakob	Gel	122-6-1, 122-7-1	Gel extraction	PP	48		NpuDnaEN_	H2O	30.0	52	30.0
427	P49-4	P49		08-15	08-15	Jakob	Gel	122-8-1, 122-9-1	Gel extraction	PP	49		NpuDnaEC_	H2O	30.0	42	30.0
428	R127	R127		08-15	01:32	MaxW	Restriction	127	PCR purification kit	Restriction	127		pSBX4C5-T7RBS-IGEMHD_v3 (E/P)	H2O	40.0	40	39.4
429	R129	R129		08-15	02:24	MaxW	Gel	123-4-1, 123-5-1	Gel extraction	Restriction	129		pSBX1K3-2Zv2Npu (E/P)	H2O	30.0	3	17.9
430	P59-5	P59		08-15	9:15	Jakob	Gel	124-2-1, 124-3-1	Gel extraction	PP	59		pSBX4A5_ccdB-BsmBI_BsaI-mRFP	H2O	30.0	105	30.0
431	P60-4	P60		08-15	9:15	Jakob	Gel	124-4-1, 124-5-1	Gel extraction	PP	60		pSBX4A5_mRFP-BsaI_BsmBI-ccdB	H2O	30.0	87	30.0
432	P69-3	P69		08-15	9:15	Jakob	Gel	124-6-1, 124-7-1	Gel extraction	PP	69		pSBX1T3_ccdB-BsmBI_BsaI-mRFP	H2O	30.0	53	30.0
433	P70-3	P70		08-15	9:15	Jakob	Gel	124-8-1, 124-9-1	Gel extraction	PP	70		pSBX1T3_mRFP-BsaI_BsmBI-ccdB	H2O	30.0	44	30.0
434	P138	P138		08-15	23:32	Consti	Gel	125-2,125-3	Gel extraction	PP	138		Xyla_BB_RBS34_X_SP	H2O	30.0	3	30.0
435	P138-2	P138		08-15	23:32	Consti	Gel	125-4,125-5	Gel extraction	PP	138		Xyla_BB_RBS34_X_SP	H2O	30.0	67	-1.0
436	P139	P139		08-15	23:32	Consti	Gel	125-6,125-7	Gel extraction	PP	139		Xyla_BsaI_circ_His	H2O	30.0	7	30.0
437	P139-2	P139		08-15	23:32	Consti	Gel	125-8,125-9	Gel extraction	PP	139		Xyla_BsaI_circ_His	H2O	30.0	20	30.0
438	P140	P140		08-15	23:32	Consti	Gel	125-11,125-12	Gel extraction	PP	140		Xyla_circ_noHis	H2O	30.0	7	30.0
439	P140-2	P140		08-15	23:32	Consti	Gel	125-14,125-14	Gel extraction	PP	140		Xyla_circ_noHis	H2O	30.0	13	30.0
442	P141-3	P141		08-15	23:32	Caro	Gel	126-2,126-3	Gel extraction	PP	141		Xyla_failed_circ_His	H2O	30.0	0	30.0
443	P36-3	P36		07-20	20:30	Maggie	Gel	34-1-2.2kb,34-3-2.2kb	Gel extraction	PP	36		pSB1A3_SortAc-H6_Smt3				0.0
444	P35-3	P35		07-20	20:30	Maggie	Gel	34-4-778bp,34-5-778bp	Gel extraction	PP	35		ccdB_Smt3-TEVc-BsaI_SortAc-H6-RFC10suffix				0.0
445	P34-3	P34		07-20	20:30	Maggie	Gel	34-6-342bp,34-7-342bp	Gel extraction	PP	34		Smt3_RFC10prefix_TEVc-BsaI				0.0
446	R130	R130		08-16	21:24	MaxW	Gel	129-2, 129-3	Gel extraction	Restriction	130		pSBX1T3-BBa_I13507 (X/P)	H2O	30.0	7	20.1
447	R132	R132		08-16	21:24	MaxW	Gel	129-6, 129-7	Gel extraction	Restriction	132		pSBX4C5-BBa_I13507 (X/P)	H2O	30.0	9	19.7
448	R133	R133		08-16	21:24	MaxW	Gel	131-2, 131-3	Gel extraction	Restriction	133		pSBX4A5-BBa_I13507 (X/P)	H2O	30.0	9	30.0
449	R134	R134		08-16	21:24	MaxW	Gel	131-4, 131-5	Gel extraction	Restriction	134		Xyla_BB_RBS34_X_SP (X/P)	H2O	30.0	7	15.7
450	R136	R136		08-16	4:10	Silvan	Restriction	136	PCR purification kit	Restriction	136		pSBX1K3-T7RBS_BBv2 (S/P)	EB	30.0		28.0
451	R137	R137		08-16	4:10	Silvan	Restriction	137	PCR purification kit	Restriction	137		pSBX1K3-T7RBS_BBv2 (S/P)	EB	30.0		28.0
452	R138	R138		08-16	4:10	Silvan	Restriction	138	PCR purification kit	Restriction	138		pSBX1K3-T7RBS_BBv2 (S/P)	EB	30.0		28.0
453	P79-4	P79		08-17	17:15	Jakob	Gel	136-2-1, 136-3-1	Gel extraction	PP	79		mRFP-NpuDnaEN-ccdB_BsaI_BsmBI	H2O	30.0	45	30.0
454	P81-4	P81		08-18	18:15	Jakob	Gel	136-4-1, 136-5-1	Gel extraction	PP	81		ccdB-NpuDnaEC-mRFP_BsmBI_BsaI	H2O	30.0	46	30.0
455	O98-3	O98		08-17	23:46	Caro	Growth	438C8	Qiagen miniprep kit	Plasmid	98	98	pSBX4C5-2Zv2Npu	H2O	30.0	357	0	164	discarded		S98
456	O98-4	O98		08-17	23:46	Caro	Growth	438C9	Qiagen miniprep kit	Plasmid	98	98	pSBX4C5-2Zv2Npu	H2O	30.0	439	19.8	165			S98	II2273	ok
457	O103-3	O103		08-17	08-17	Caro	Growth	443C1	Qiagen miniprep kit	Plasmid	103	103	pSBX1K3-2Zv2Npu	H2O	30.0	162	10.5	168			A103#2
458	O110	O110		08-17	08-17	Caro	Growth	444C1	Qiagen miniprep kit	Plasmid	110	110	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	H2O	30.0	325	0	169	discarded		G110
459	O111	O111		08-17	08-17	Caro	Growth	445C1	Qiagen miniprep kit	Plasmid	111	111	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	H2O	30.0	323	29.0	170	discarded		G111
460	O112	O112		08-17	08-17	Caro	Growth	446C2	Qiagen miniprep kit	Plasmid	112	112	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	H2O	30.0	229	0	171	discarded		G112
461	O113	O113		08-17	08-17	Caro	Growth	447C1	Qiagen miniprep kit	Plasmid	113	113	pSBX1K3-2Zv2Npu:Xyla_circ_noHis	H2O	30.0	217	0	172	discarded		G113
462	O109	O109		08-18	15:30	Lisa	Growth	458C1	Qiagen miniprep kit	Plasmid	109	109	pSB1C3-BBa_K808000	H2O	30.0	375	24.7	179			D2014-2-7E	II2288	ok
463	O109-2	O109		08-18	15:30	Lisa	Growth	458C2	Qiagen miniprep kit	Plasmid	109	109	pSB1C3-BBa_K808000	H2O	30.0	122	0	180	discarded		D2014-2-7E
464	O109-3	O109		08-18	15:30	Lisa	Growth	458C3	Qiagen miniprep kit	Plasmid	109	109	pSB1C3-BBa_K808000	H2O	30.0	334	0	181	discarded		D2014-2-7E
465	O117	O117		08-18	15:30	Lisa	Growth	467C1	Qiagen miniprep kit	Plasmid	117	117	pSB1C3-BBa_K741002	H2O	30.0	224	0	182	discarded		D2013-2-17M
466	O117-2	O117		08-18	15:30	Lisa	Growth	467C2	Qiagen miniprep kit	Plasmid	117	117	pSB1C3-BBa_K741002	H2O	30.0	259	22.3	183			D2013-2-17M	II2289	ok
467	O117-3	O117		08-18	15:30	Lisa	Growth	467C3	Qiagen miniprep kit	Plasmid	117	117	pSB1C3-BBa_K741002	H2O	30.0	169	30.0	184	discarded		D2013-2-17M
468	P141-4	P141		08-18		Consti	Purification	P141-3	Dilution	PP	141		Xyla_failed_circ_His	H2O	10.0		10.0		1:10 Dilution of P141-3 because conc. could be too high for another PCR
469	O103-4	O103		08-16		MaxW	Growth	433C4-1	Qiagen miniprep kit	Plasmid	103	103	pSBX1K3-2Zv2Npu	H2O	50.0	87	50.0		reprep of O103-2 (pos sequenzing)		A103
470	O55-5	O55		08-18	15:45	Silvan	Growth	448C1	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)			338	-2.0	106			S38	II2281
471	O55-6	O55		08-18	08-18	Silvan	Growth	448C2	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)			154	-2.0	107			S38	II2282
472	O55-7	O55		08-18	16:00	Silvan	Growth	448C3	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)			132	-2.0	173			S38	II2283
473	O55-8	O55		08-18	16:00	Silvan	Growth	448C4	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)			162	-2.0	174			S38	II2284
474	O116	O116		08-18	16:00	Silvan	Growth	453C3	Qiagen miniprep kit	Plasmid	116	116	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)			223	-2.0	175	discarded		S116	II2285
475	O116-2	O116		08-18	16:00	Silvan	Growth	454C1	Qiagen miniprep kit	Plasmid	116	116	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)			190	-2.0	176	discarded		S116#2	II2286	single base deletion
476	O116-3	O116		08-18	16:00	Silvan	Growth	455C1	Qiagen miniprep kit	Plasmid	116	116	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)			243	-2.0	177	discarded		S116#3	II2287	two-base deletion and point mutation
477	O116-4	O116		08-18	16:00	Silvan	Growth	455C3	Qiagen miniprep kit	Plasmid	116	116	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)			38	-2.0	178	discarded		S116#3
478	R140	R140		08-18	18:00	Charlotte	Restriction	140	PCR purification kit	Restriction	140		pSB1AK8-BBa_I712074 (S/P)	H2O	50.0	16	47.4
479	R141	R141		08-18	18:00	Charlotte	Gel	140-2-2, 140-3-2	Gel extraction	Restriction	141		pSBX4C5-2ZSspH6:GFP (X/P)	H2O	50.0	11	45.8
480	P141-5	P141		08-18	18:33	Caro	PCR	479	PCR purification kit	PP	141		Xyla_failed_circ_His	H2O	30.0		30.0
481	P141-6	P141		08-18	18:37	Caro	PCR	480	PCR purification kit	PP	141		Xyla_failed_circ_His	H2O	30.0		30.0
482	P141-7	P141		08-18	19:11	Consti	Gel	142-3, 142-4	Gel extraction	PP	141		Xyla_failed_circ_His	H2O	30.0	30	30.0
483	P138-3	P138		08-18	21:18	Caro	PCR	482	PCR purification kit	PP	138		Xyla_BB_RBS34_X_SP	H2O	30.0		15.0
484	R145	R145		08-18	21:23	Caro	Restriction	145	PCR purification kit	Restriction	145		Xyla_BB_RBS34_X_SP (X/P)	H2O	30.0		30.0
485	R139	R139		08-18	21:44	Caro	Restriction	139	Gel extraction	Restriction	139		pSBX1T3-BBa_I13507 (X/P)	H2O	30.0	5	16.2
486	R142	R142		08-18	21:44	Caro	Restriction	142	Gel extraction	Restriction	142		pSBX4A5-BBa_I13507 (X/P)	H2O	30.0	14	19.4
487	R143	R143		08-18	21:44	Caro	Restriction	143	Gel extraction	Restriction	143		pSBX4C5-BBa_I13507 (X/P)	H2O	30.0	12	18.9
488	R144	R144		08-18	21:44	Caro	Restriction	144	Gel extraction	Restriction	144		pSBX1K3-BBa_I13507 (X/P)	H2O	30.0		30.0
489	R145-2	R145		08-18	21:44	Caro	Restriction	145	Gel extraction	Restriction	145		Xyla_BB_RBS34_X_SP (X/P)	H2O	30.0	5	19.2
490	P39	P39		08-18	1:00	Silvan	Gel	145-2-x,145-3-x	Gel extraction	PP	39		ccdB_pSB*3-BsaI_SortAc-His6	EB	30.0	172	30.0
491	P148	P148		08-18	1:00	Silvan	Gel	145-4-x,145-5-x	Gel extraction	PP	148		mRFPexp_BsaI=CGCA=BsmBI_BsmBI=CTTC=BsaI	EB	30.0	124	30.0
492	P147	P147		08-18	1:00	Silvan	Gel	145-8-x,145-9-x	Gel extraction	PP	147		mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaI	EB	30.0	30	1.9
493	P151	P151		08-19	21:20	Lisa	Gel	147-4, 147-5	Gel extraction	PP	151		pSBX1K3-T7RBS_taataa-iGEMHD_BsaI-mRFP	H2O	30.0	82	28.0
494	P152-0			08-20	10:00	Lisa	Gel	151-2, 151-3	Gel extraction	PP	152		pSBX1K3-T7RBS_mRFP-BsaI_atg	H2O	30.0	55	28.0
495	P153	P153		08-19	23:45	Caro	Gel	148-2,148-3,148-5,148-6,148-10,148-11	Gel extraction	PP	153		mRFP-NpuDnaEN-ccdB_X_SP	H2O	30.0	37	3.0
496	P154	P154		08-19	23:45	Caro	Gel	148-4,148-5,148-8,148-9,148-12,148-13	Gel extraction	PP	154		ccdB-NpuDnaEC-mRFP_X_SP	H2O	30.0	51	10.4
497	R151	R151		08-20	01:26	Caro	Restriction	151	PCR purification kit	Restriction	151		mRFP-NpuDnaEN-ccdB_X_SP (X/P)	H2O	30.0	30	4.2
498	R152	R152		08-20	01:26	Caro	Restriction	152	PCR purification kit	Restriction	152		ccdB-NpuDnaEC-mRFP_X_SP (X/P)	H2O	30.0	43	10.5
499	R157	R157		08-20		Charlotte	Gel	153-4, 154-2, 154-3	Gel extraction	Restriction	157		pSBX4C5-2ZSspH6:GFP (X/P)	H2O	30.0	13	21.7
500	R156	R156		08-20		Charlotte	Gel	153-2, 153-3	Gel extraction	Restriction	156		pSB1AK8-BBa_I712074 (S/P)	H2O	30.0	15	27.7
501	R158	R158		08-20		Charlotte	Restriction	158	PCR purification kit	Restriction	158		pSB1AK8-BBa_I712074 (S/P)	H2O	30.0	4	25.4
502	R158-2	R158		08-20		Charlotte	Restriction	158	PCR purification kit	Restriction	158		pSB1AK8-BBa_I712074 (S/P)	H2O	30.0	3	25.0		phosphatase was added
503	R160	R160		08-20	17:06	Caro	Gel	155-2	Gel extraction	Restriction	160		pSBX1T3-BBa_I13507 (X/P)	H2O	30.0	9	5.8
504	R162	R162		08-20	17:06	Caro	Gel	155-4	Gel extraction	Restriction	162		pSBX4C5-BBa_I13507 (X/P)	H2O	30.0	13	6.9
505	O55-9	O55		08-20	18:20	Silvan	Growth	211C5	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	259	38.2				A55	76DB25, 76DB27, 76DB63	ok, bad sequencing, ok
506	O55-10	O55		08-20	18:20	Silvan	Growth	212C6	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	226	48.0				A55#2	76DB26	mixture
507	P153-2	P153		08-20	19:00	Jakob	Gel	158-2-1, 158-3-1	Gel extraction	PP	153		mRFP-NpuDnaEN-ccdB_X_SP	H2O	30.0	31	13.7
508	P154-2	P154		08-20	19:00	Jakob	Gel	158-4-1, 158-5-1	Gel extraction	PP	154		ccdB-NpuDnaEC-mRFP_X_SP	H2O	30.0	29	12.7
509	O94	O94		08-20	21:09	Caro	Ligation	S94#2	PCR purification kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0		0
510	O92	O92		08-20	21:09	Caro	Ligation	S92#2	PCR purification kit	Plasmid	92	92	pSBX1T3-2GateNpuDnaE(N)	H2O	30.0		0
511	O95	O95		08-20	21:09	Caro	Ligation	S95#2	PCR purification kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0		0
512	O93	O93		08-20	21:09	Caro	Ligation	S93#2	PCR purification kit	Plasmid	93	93	pSBX1T3-2GateNpuDnaE(C)	H2O	30.0		0
513	O94-2	O94		08-20	21:09	Caro	Ligation	S94#3	PCR purification kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0		0
514	O92-2	O92		08-20	21:09	Caro	Ligation	S92#3	PCR purification kit	Plasmid	92	92	pSBX1T3-2GateNpuDnaE(N)	H2O	30.0		0
515	O95-2	O95		08-20	21:09	Caro	Ligation	S95#3	PCR purification kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0		0
516	O93-2	O93		08-20	21:09	Caro	Ligation	S93#3	PCR purification kit	Plasmid	93	93	pSBX1T3-2GateNpuDnaE(C)	H2O	30.0		0
517	O104-2	O104		08-20	22:11	Jan	Purification	O104	Dilution	Plasmid	104	104	pSB1C3-BBa_I746908	H2O	97.0	1	91.0
518	R166	R166		08-20	23:30	Jakob	Restriction	166	PCR purification kit	Restriction	166		mRFP-NpuDnaEN-ccdB_X_SP (X/P)	H2O	30.0	5	-6.7		dont believe it
519	R167	R167		08-20	23:30	Jakob	Restriction	167	PCR purification kit	Restriction	167		ccdB-NpuDnaEC-mRFP_X_SP (X/P)	H2O	30.0	6	-5.0		dont believe it
520	P132	P132		08-21	04:58	Jan	Gel	159-6-1,159-7-1	Gel extraction	PP	132		sfGFP^N_T7RBS-H6_C->G-NpuDnaE^N	H2O	30.0	82	30.0
521	P129	P129		08-21	04:58	Jan	Gel	159-8-1,159-9-1	Gel extraction	PP	129		sfGFP^N_T7RBS-H6_NpuDnaE^N	H2O	30.0	63	30.0
522	P125	P125		08-21	04:58	Jan	Gel	160-2-1,160-3-1	Gel extraction	PP	125		sfGFP^C_NpuDnaE^C_H6	H2O	30.0	230	30.0
523	P130	P130		08-21	04:58	Jan	Gel	160-4-1,160-5-1	Gel extraction	PP	130		pSBX1K3-T7RBS_H6-NpuDnaE^N_H6	H2O	30.0	66	30.0
524	P126	P126		08-21	04:58	Jan	Gel	160-6-1,160-7-1	Gel extraction	PP	126		pSB_H6-sfGFP^C_NpuDnaE^C-H6	H2O	30.0	71	30.0
525	O27-2	O27		08-21	15:30	Lisa	Purification	O27	Dilution	Plasmid	27	27	pCL20	H2O	40.0	1	36.0
526	O110-2	O110		08-21	16:30	MaxW	Growth	476C1	Qiagen miniprep kit	Plasmid	110	110	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	H2O	50.0	320	0	196	discarded		G110#2	76DB28	several deletions
527	O110-3	O110		08-21	16:30	MaxW	Growth	476C2	Qiagen miniprep kit	Plasmid	110	110	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	H2O	50.0	281	0	197	discarded		G110#2	76DB77, 76DB82	a deletion, a deletion
528	R153	R153		08-21	18:00	Silvan	Gel	163-1-100bp, 163-2-100bp, 163-3-100bp	Gel extraction	Restriction	153		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (X/BglII)	EB	25.0	1	19.4		measured 9.7 (impossible)
529	R154	R154		08-21	18:00	Silvan	Gel	163-5-x, 163-7-x, 163-8-x	Gel extraction	Restriction	154		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (BglII/P)	EB	25.0	10	19.9
530	P158	P158		08-21	19:40	Lisa	Gel	158	Gel extraction	PP	158		SspDnaBC_M+H6_N->A+SGG	H2O	30.0	85	6.4
531	P159	P159		08-21	19:40	Lisa	Gel	159	Gel extraction	PP	159		SspDnaBC_M-noH6_N->A+SGG	H2O	30.0	83	5.8
532	P124	P124		08-21	20:00	Jan	Gel	164-2-1,164-3-1	Gel extraction	PP	124		NpuDnaE^C_H6_sfGFP	H2O	20.0		0		lower band, discarded and redone
533	P127	P127		08-21	20:00	Jan	Gel	164-4-1,164-5-1	Gel extraction	PP	127		NpuDnaE^C SN->AG_H6_sfGFP	H2O	20.0		0		lower band, discarded and redone
534	P124-2	P124		08-21	20:00	Jan	Gel	164-2-2,164-3-2	Gel extraction	PP	124		NpuDnaE^C_H6_sfGFP	H2O	20.0		0		upper band, discarded and redone
535	P127-2	P127		08-21	20:00	Jan	Gel	164-4-2,164-5-2	Gel extraction	PP	127		NpuDnaE^C SN->AG_H6_sfGFP	H2O	20.0		0		upper band, discarded and redone
536	O30-4	O30		08-21	20:26	Jan	Purification	O30	Dilution	Plasmid	30	30	pVS07	H20	150.0	1	130.0
537	R168	R168		08-22	1:00	Jakob	Gel	168-2-1, 168-3-1	Gel extraction	Restriction	168		pSBX1K3-BBa_I13507 (X/P)	H2O	30.0	11	26.6
538	R169	R169		08-22	1:00	Jakob	Gel	168-4-1, 168-5-1	Gel extraction	Restriction	169		pSBX1T3-BBa_I13507 (X/P)	H2O	30.0	11	26.4
539	R170	R170		08-22	1:00	Jakob	Gel	168-6-1, 168-7-1	Gel extraction	Restriction	170		pSBX4A5-BBa_I13507 (X/P)	H2O	30.0	9	24.6
540	R171	R171		08-22	1:00	Jakob	Gel	168-9-1, 168-10-1	Gel extraction	Restriction	171		pSBX4C5-BBa_I13507 (X/P)	H2O	30.0	13	26.2
541	O122	O122		08-22	11:00	Lisa	Growth	485C1	Qiagen miniprep kit	Plasmid	122	122	pSBX1K3-1GateNpuDnaE(N)	H2O	30.0	302	29.3	199	with igemhd		A122
542	O123	O123		08-22	11:00	Lisa	Growth	486C1	Qiagen miniprep kit	Plasmid	123	123	pSBX1K3-1GateNpuDnaE(C)	H2O	30.0	310	29.4	200	with igemhd		A123	II2290, II2291	mutation A220G K74E in the mRFP CDS, besides ok, mutation A220G K74E in the mRFP CDS, besides ok
543	O123-2	O123		08-22	11:00	Lisa	Growth	486C2	Qiagen miniprep kit	Plasmid	123	123	pSBX1K3-1GateNpuDnaE(C)	H2O	30.0	165	28.8	201	with igemhd		A123
544	O86-2	O86		08-22	12:00	Charlotte	Growth	508C2	Qiagen miniprep kit	Plasmid	86	86	pSB1AK8-BBa_I712074-2ZSspH6:GFP	H2O	50.0		50.0				S86#4
545	O117-4	O117		08-22	20:40	Lisa	Purification	O117-2	Dilution	Plasmid	117	117	pSB1C3-BBa_K741002	H2O	100.0	3	91.0
546	P128	P128		08-23	18:28	Jan	Gel	173-1-1,173-2-1	Gel extraction	PP	128		NpuDnaE^N_sfGFP^N_H6	H2O	30.0	75	28.0
547	P131	P131		08-23	18:28	Jan	Gel	173-3-1,173-4-1	Gel extraction	PP	131		C->G-NpuDnaE^N_sfGFP_H6	H2O	30.0	98	28.0
548	P40	P40		08-23	18:28	Jan	Gel	178-1-1	Gel extraction	PP	40		pSB1C3_ccdB_SortAc-His6	H2O	30.0	45	28.0
549	O29-4	O29		08-23	18:28	Jan	Purification	O29	Dilution	Plasmid	29	29	pVS41	H2O	114.0	1	68.0
550	P124-3	P124		08-23	23:21	Jan	PCR	658	PCR purification kit	PP	124		NpuDnaE^C_H6_sfGFP	H2O	20.0	58	18.0
551	P127-3	P127		08-23	23:21	Jan	PCR	660	PCR purification kit	PP	127		NpuDnaE^C SN->AG_H6_sfGFP	H2O	20.0	83	18.0
552	O111-2	O111		08-24	0:56	MaxW	Growth	501C3	Qiagen miniprep kit	Plasmid	111	111	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	H2O	50.0	81	50.0	204			G111#2	II2294, II2295	EINE, 1, EINE fucking mutation........., EINE, 1, EINE fucking mutation.........
553	O113-2	O113		08-24	0:56	MaxW	Growth	503C5	Qiagen miniprep kit	Plasmid	113	113	pSBX1K3-2Zv2Npu:Xyla_circ_noHis	H2O	50.0	299	0	205	discarded		G113#2	II2298, II2299	fürn arsch, fürn arsch
554	O112-2	O112		08-24	0:56	MaxW	Growth	502C6	Qiagen miniprep kit	Plasmid	112	112	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	H2O	50.0	349	48.6	206			G112#2	II2296, II2297	ok, ok
555	O112-3	O112		08-24	0:56	MaxW	Growth	502C7	Qiagen miniprep kit	Plasmid	112	112	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	H2O	50.0	264	0	207	discarded		G112#2
556	O112-4	O112		08-24	0:56	MaxW	Growth	502C8	Qiagen miniprep kit	Plasmid	112	112	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	H2O	50.0	293	0	208	discarded		G112#2
557	O124	O124		08-24	0:56	MaxW	Growth	504C6	Qiagen miniprep kit	Plasmid	124	124	pSBX1K3-2Zv2Npu:Xyla(H6)destrCext	H2O	50.0	272	46.0	209			G124	76DB73, 76DB78	insertions, insertions
558	O124-2	O124		08-24	0:56	MaxW	Growth	504C7	Qiagen miniprep kit	Plasmid	124	124	pSBX1K3-2Zv2Npu:Xyla(H6)destrCext	H2O	50.0	286	50.0	210			G124	II2300, II2301	fürn arsch, fürn arsch
559	O124-3	O124		08-24	0:56	MaxW	Growth	504C8	Qiagen miniprep kit	Plasmid	124	124	pSBX1K3-2Zv2Npu:Xyla(H6)destrCext	H2O	50.0	237	43.9	211			G124	76DB74, 76DB79	ok, ok
560	O124-4	O124		08-24	0:56	MaxW	Growth	504C9	Qiagen miniprep kit	Plasmid	124	124	pSBX1K3-2Zv2Npu:Xyla(H6)destrCext	H2O	50.0	266	50.0	212			G124
561	O125	O125		08-24	0:56	MaxW	Growth	512C5	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	50.0	250	46.0	213			L125	76DB29, 76DB30	negative, contains GFP, negative, contains GFP
562	O125-2	O125		08-24	0:56	MaxW	Growth	512C6	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	50.0	271	47.0	214			L125	76DB31, 76DB32	negative, contains GFP, negative, contains GFP
563	O125-3	O125		08-24	0:56	MaxW	Growth	512C7	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	50.0	375	47.6	215			L125	76DB33, 76DB34	negative, contains GFP, negative, contains GFP
564	O94-3	O94		08-24	1:30	Jakob	Growth	525C1	Qiagen miniprep kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0	306	29.0				S94#4	II2302, II2306	bad sequencing, but probaBIy ok, bad sequencing, but probaBIy ok
565	O94-4	O94		08-24	1:30	Jakob	Growth	525C2	Qiagen miniprep kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0	250	0		discarded		S94#4	II2303, II2307	bad sequencing, but probaBIy ok, bad sequencing, but probaBIy ok
566	O94-5	O94		08-24	1:30	Jakob	Growth	525C3	Qiagen miniprep kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0	311	0		discarded		S94#4
567	O94-6	O94		08-24	1:30	Jakob	Growth	525C4	Qiagen miniprep kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0	353	0		discarded		S94#4
568	O94-7	O94		08-24	1:30	Jakob	Growth	525C5	Qiagen miniprep kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0	343	0		discarded		S94#4
569	O95-3	O95		08-24	1:30	Jakob	Growth	526C1	Qiagen miniprep kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0	278	29.0				S95#4	II2304, II2308	bad sequencing, but probaBIy ok, bad sequencing, but probaBIy ok
570	O95-4	O95		08-24	1:30	Jakob	Growth	526C2	Qiagen miniprep kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0	306	0		discarded		S95#4	II2305, II2309	bad sequencing, but probaBIy ok, bad sequencing, but probaBIy ok
571	O95-5	O95		08-24	1:30	Jakob	Growth	526C3	Qiagen miniprep kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0	359	0		discarded		S95#4
572	O95-6	O95		08-24	1:30	Jakob	Growth	526C4	Qiagen miniprep kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0	212	0		discarded		S95#4
573	O95-7	O95		08-24	1:30	Jakob	Growth	526C5	Qiagen miniprep kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0	309	0		discarded		S95#4
574	P152	P152		08-24	22:20	MaxW	Gel	186-2	Gel extraction	PP	152		pSBX1K3-T7RBS_mRFP-BsaI_atg	H2O	30.0	48	30.0
575	P166	P166		08-25	1:16	Jan	Gel	192-1-1,192-2-1	Gel extraction	PP	166		sfGFP^N_T7RBS-H6_H6	H2O	20.0	172	18.0
576	P167	P167		08-25	1:16	Jan	Gel	192-3-1,192-4-1	Gel extraction	PP	167		pSBX1K3-T7RBS_sfGFP^C-H6_H6	H2O	20.0	88	18.0
577	O86-3	O86		08-25	1:42	Charlotte	Growth	510C2	Qiagen miniprep kit	Plasmid	86	86	pSB1AK8-BBa_I712074-2ZSspH6:GFP	H2O	30.0	360	30.0	218	for sequencing		S86#5	II2310, II2311	ok, ok
578	O105	O105		08-25	18:00	Caro	Growth	527C2	Qiagen miniprep kit	Plasmid	105	105	pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	H2O	30.0	200	0	219	discarded		A105
579	O105-2	O105		08-25	18:00	Caro	Growth	527C4	Qiagen miniprep kit	Plasmid	105	105	pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	H2O	30.0	222	14.3	220			A105	II2312, II2313	ok, ok, C->T in VF2 site
580	O106	O106		08-25	18:00	Caro	Growth	528C1	Qiagen miniprep kit	Plasmid	106	106	pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6	H2O	30.0	210	25.5	221			A106	76DC22	ok
581	O106-2	O106		08-25	18:00	Caro	Growth	528C3	Qiagen miniprep kit	Plasmid	106	106	pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6	H2O	30.0	287	0	222	discarded		A106	II2314, II2315	mixture A64->T mutation, mixture A64->Tmutation
582	O107	O107		08-25	18:00	Caro	Growth	529C1	Qiagen miniprep kit	Plasmid	107	107	pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	H2O	30.0	270	18.9	223	needs to be found or reperped		A107	II2316, II2317	ok, good, ok, C->T in VF2 site
583	O107-2	O107		08-25	18:00	Caro	Growth	529C2	Qiagen miniprep kit	Plasmid	107	107	pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	H2O	30.0	255	30.0	224	discarded		A107
584	O108	O108		08-25	18:00	Caro	Growth	530C3	Qiagen miniprep kit	Plasmid	108	108	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	H2O	30.0	237	13.1	225			A108	II2318, II2319	1bp deletion before final H6, 1bp deletion in suffix, 1bp deletion before final H6, 1bp deletion in suffix
585	O108-2	O108		08-25	18:00	Caro	Growth	530C2	Qiagen miniprep kit	Plasmid	108	108	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	H2O	30.0	301	23.7	226			A108	76DC11, 76DC12	mixture with T7RBS T6del, mixture with T7RBS T6del
586	O128	O128		08-26	11:00	Caro	Growth	531C1	Qiagen miniprep kit	Plasmid	128	128	pSBX1K3-T7RBS-H6-sfGFP-H6	H2O	30.0	246	26.3				A128	76DB36, 76DB37	perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg, perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg
587	O128-2	O128		08-26	11:00	Caro	Growth	532C1	Qiagen miniprep kit	Plasmid	128	128	pSBX1K3-T7RBS-H6-sfGFP-H6	H2O	30.0	133	25.6				A128#2	76DC15	deletion in first stop codon, VF2C7T
588	O128-3	O128		08-26	11:00	Caro	Growth	533C1	Qiagen miniprep kit	Plasmid	128	128	pSBX1K3-T7RBS-H6-sfGFP-H6	H2O	30.0	198	27.6				A128#3	76DC16	ok, VF2C7T
589	O122-2	O122		08-26	11:00	Caro	Growth	534C1	Qiagen miniprep kit	Plasmid	122	122	pSBX1K3-1GateNpuDnaE(N)	H2O	30.0	185	30.0		discard		A122#3
590	O122-3	O122		08-26	11:00	Caro	Growth	534C2	Qiagen miniprep kit	Plasmid	122	122	pSBX1K3-1GateNpuDnaE(N)	H2O	30.0	286	28.3				A122#3	II2320, II2321	ok, perfect, ok, perfect
591	O123-3	O123		08-26	11:00	Caro	Growth	535C1	Qiagen miniprep kit	Plasmid	123	123	pSBX1K3-1GateNpuDnaE(C)	H2O	30.0	115	30.0		discard		A123#2
592	O123-4	O123		08-26	11:00	Caro	Growth	535C2	Qiagen miniprep kit	Plasmid	123	123	pSBX1K3-1GateNpuDnaE(C)	H2O	30.0	188	30.0		discard		A123#2
593	O123-5	O123		08-26	11:00	Caro	Growth	535C3	Qiagen miniprep kit	Plasmid	123	123	pSBX1K3-1GateNpuDnaE(C)	H2O	30.0	208	27.6				A123#2	II2322, II2323	ok, perfect, ok, perfect
594	R183	R183		08-26	15:00	Caro	Gel	195-2	Gel extraction	Restriction	183		pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 (X/P)	H2O	30.0	8	26.5
595	R184	R184		08-26	15:00	Caro	Gel	195-3	Gel extraction	Restriction	184		pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (X/P)	H2O	30.0	7	25.4
596	R185	R185		08-26	15:00	Caro	Restriction	185	PCR purification kit	Restriction	185		pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 (S/P)	H2O	30.0	9	26.7
597	R186	R186		08-26	15:00	Caro	Restriction	186	PCR purification kit	Restriction	186		pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)	H2O	30.0	14	27.8
598	P173	P173		08-26	6:30 PM	MaxW	PCR	739	PCR purification kit	PP	173		LambdaLys_BsaI_c-ext_BsaI	H2O	30.0	21	15.9
599	P174	P174		08-26	18:30	MaxW	PCR	740	PCR purification kit	PP	174		ccdB_BsmBI-BsaI_BsaI-BsmBI	H2O	30.0	112	18.4
600	O94-8	O94		08-26	10:00 PM	Jakob	Purification	O94-3	Dilution	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	306.0	1	278.8
601	O95-8	O95		08-26	10:00 PM	Jakob	Purification	O95-3	Dilution	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	278.0	1	250.8
602	R187	R187		08-26	22:15	Silvan	Gel	197-1-600bp,197-2-600bp	Gel extraction	Restriction	187		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (E/XmaI)	EB	30.0	8	25.0
603	R188	R188		08-26	22:15	Silvan	Gel	197-4-500bp,197-5-500bp	Gel extraction	Restriction	188		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (XmaI/P)	EB	30.0	7	25.6
604	P176	P176		08-27	10:00 AM	Jakob	Gel	208-2-1	Gel extraction	PP	176		BsmBI-ccdB-BsmBI_pSBX4C5_BsaI-mRFP-BsaI	H2O	30.0	12	30.0
605	P177	P177		08-27	10:00 AM	Jakob	Gel	208-3-1	Gel extraction	PP	177		BsaI-mRFP-BsaI_pSBX4C5_BsmBI-ccdB-BsmBI	H2O	30.0	22	30.0
606	P175	P175		08-27	10:30	Lisa	Gel	202-2, 202-3	Gel extraction	PP	175		pSB1C3-BBa_K741002_MT_SspDnaBN	H2O	30.0	19	28.0
607	P187	P187		08-27	13:40	Lisa	Oligos	185,193	Annealing	PP	187		CPEC_GFP_Insert_oligo			25	0.0
608	O55-11	O55		08-27		Silvan	Growth	209C8	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	41	19.1				A55	76DB38	single base insertion in Smt3/ccdb CPEC homology region
609	O55-12	O55		08-27		Silvan	Growth	211C6	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	243	28.1				A55	76DB39	single base deletion in Smt3/ccdb CPEC homology region
610	O55-13	O55		08-27		Silvan	Growth	211C7	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	292	26.9				A55	76DB40, 76DB64	ok, ok
611	O55-14	O55		08-27		Silvan	Growth	211C8	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	235	26.2				A55	76DB41, 76DB65	ok, ok
612	O55-15	O55		08-27		Silvan	Growth	211C9	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	276	26.7				A55	76DB42, 76DB66	ok, bad sequencing
613	O55-16	O55		08-27		Silvan	Growth	211C10	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	216	25.8				A55	76DB43, 76DB67	ok, ok
614	O55-17	O55		08-27		Silvan	Growth	211C11	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	355	28.7				A55	76DB44	single base deletion in backbone/Smt3 CPEC homology region
615	O55-18	O55		08-27		Silvan	Growth	212C7	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	381	27.6				A55#2	76DB45, 76DB68	ok, ok (one T not called)
616	O55-19	O55		08-27		Silvan	Growth	212C8	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	242	26.3				A55#2	76DB46, 76DB69	ok, ok
617	O55-20	O55		08-27		Silvan	Growth	212C9	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	227	-11.4				A55#2	76DB47, 76DB70	ok, ok
618	O55-21	O55		08-27		Silvan	Growth	212C10	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	239	26.2				A55#2	76DB48, 76DB71	ok, mixture
619	O55-22	O55		08-27		Silvan	Growth	212C11	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	235	26.2				A55#2	76DB49, 76DB72	ok, ok
620	O55-23	O55		08-27		Silvan	Growth	212C12	Qiagen miniprep kit	Plasmid	55	55	pSB1A3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	253	26.4				A55#2	76DB50, 76DB34	ok, ok
621	O125-4	O125		08-27		Silvan	Growth	544C1	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	143	23.7	227			L125#2	76DB51, 76DB57	negative, contains GFP, negative, contains GFP
622	O125-5	O125		08-27		Silvan	Growth	544C2	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	120	22.5	228			L125#2	76DB52, 76DB58	negative, contains GFP, negative, contains GFP
623	O125-6	O125		08-27		Silvan	Growth	544C3	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	104	21.3	229			L125#2	76DB53, 76DB59	negative, contains GFP, negative, contains GFP
624	O125-7	O125		08-27		Silvan	Growth	544C4	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	97	20.8	230			L125#2	76DB54, 76DB60	negative, contains GFP, negative, contains GFP
625	O125-8	O125		08-27		Silvan	Growth	544C5	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	77	18.3	231			L125#2	76DB55, 76DB61	negative, contains GFP, negative, contains GFP
626	O125-9	O125		08-27		Silvan	Growth	544C6	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	H2O	30.0	166	24.6	232			L125#2	76DB56, 76DB62	negative, contains GFP, negative, contains GFP
627	R189	R189		08-27		Anna	Gel	205-2, 205-3	Gel extraction	Restriction	189		pSB1AK8-BBa_I712074 (S/P)	H2O	30.0	11	15.4
628	R190	R190		08-27		Anna	Gel	205-4, 205-5	Gel extraction	Restriction	190		pSB1A2-BBa_I13507 (X/P)	H2O	30.0	10	17.9
629	O94-9	O94		08-27	11:00 PM	Jakob	Growth	525C1-1	Qiagen miniprep kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0	291	30.0	233		y	S94#4
630	O94-10	O94		08-27	11:00 PM	Jakob	Growth	525C2-1	Qiagen miniprep kit	Plasmid	94	94	pSBX4C5-2GateNpuDnaE(N)	H2O	30.0	350	26.6	234		y	S94#4
631	O95-9	O95		08-27	11:00 PM	Jakob	Growth	526C1-1	Qiagen miniprep kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0	263	30.0	235		y	S95#4
632	O95-10	O95		08-27	11:00 PM	Jakob	Growth	526C2-1	Qiagen miniprep kit	Plasmid	95	95	pSBX4C5-2GateNpuDnaE(C)	H2O	30.0	216	24.4	236		y	S95#4
633	R199	R199		08-27	11:00 PM	Caro	Gel	210-2, 210-3	Gel extraction	Restriction	199		pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 (X/P)	H2O	30.0	8	24.6
634	R200	R200		08-27	11:00 PM	Caro	Gel	210-4,210-5	Gel extraction	Restriction	200		pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (X/P)	H2O	30.0	8	24.0
635	R185-2	R185		08-27	11:00 PM	Caro	Restriction	185	PCR purification kit	Restriction	185		pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 (S/P)	H2O	30.0	2	24.6
636	R186-2	R186		08-27	11:00 PM	Caro	Restriction	186	PCR purification kit	Restriction	186		pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)	H2O	30.0	4	25.3
637	R185-3	R185		08-27	11:00 PM	Caro	Restriction	185	PCR purification kit	Restriction	185		pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 (S/P)	H2O	30.0	12	27.3
638	R186-3	R186		08-27	11:00 PM	Caro	Restriction	186	PCR purification kit	Restriction	186		pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)	H2O	30.0	11	27.2
639	R203	R203		08-28	0:12	MaxW	Oligos	94,95	Annealing	Restriction	203		AssemBIyIntN-linker+H6 ("GATG"/"GGTG")	T4L Buffer	100.0	17	100.0
640	R204	R204		08-28	0:12	MaxW	Oligos	96,97	Annealing	Restriction	204		AssemBIyIntC-linker+H6 ("GATG"/"GGTG")	T4L Buffer	100.0	15	100.0
641	P181	P181		08-28	2:00 AM	Jakob	Oligos	86,87	Mix	PP	181		SspDnaX-S11C	H2O DNase free ;-)			0.0
642	P183	P183		08-28	2:00 AM	Jakob	Oligos	88,89	Mix	PP	183		Ssp GyrB-S11C	H2O DNase free ;-)			0.0
643	P185	P185		08-28	2:00 AM	Jakob	Oligos	90,91	Mix	PP	185		Ter DnaE3-S11C	H2O DNase free ;-)			0.0
644	P178	P178		08-28	2:00 AM	Jakob	Extern	GeneArt	Resuspended	PP	178		gp41-1N	H2O DNase free ;-)	15.0	50	15.0
645	P179	P179		08-28	2:00 AM	Jakob	Extern	GeneArt	Resuspended	PP	179		gp41-1C	H2O DNase free ;-)	10.0	50	10.0
646	P180	P180		08-28	2:00 AM	Jakob	Extern	GeneArt	Resuspended	PP	180		Ssp DnaX-S11N	H2O DNase free ;-)	33.0	50	33.0
647	P182	P182		08-28	2:00 AM	Jakob	Extern	GeneArt	Resuspended	PP	182		Ssp GyrB-S11N	H2O DNase free ;-)	25.0	50	25.0
648	P184	P184		08-28	2:00 AM	Jakob	Extern	GeneArt	Resuspended	PP	184		Ter DnaE3-S11N	H2O DNase free ;-)	21.0	50	21.0
649	O131	O131		08-28	15:30	MaxW	Growth	543C5	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	30.0		0	238	discarded		G131	76DB75, 76DB80	gfp..............................., gfp...............................
650	O131-2	O131		08-28	15:30	MaxW	Growth	543C7	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	30.0		0	239	discarded		G131	76DB76, 76DB81	ccdb missing, ccdb missing
651	O132	O132		08-28	16:30	Lisa	Growth	547C1	Qiagen miniprep kit	Plasmid	132	132	pSB1C3-Plac-GFP-IntN-MTS	H2O	30.0	194	25.4	240	discarded		A132	76DB83, 76DB84	is actually psbx ssp h6 gfp, is actually psbx ssp h6 gfp
652	O132-2	O132		08-28	16:30	Lisa	Growth	547C2	Qiagen miniprep kit	Plasmid	132	132	pSB1C3-Plac-GFP-IntN-MTS	H2O	30.0	176	30.0	241	discarded		A132
653	O132-3	O132		08-28	16:30	Lisa	Growth	547C3	Qiagen miniprep kit	Plasmid	132	132	pSB1C3-Plac-GFP-IntN-MTS	H2O	30.0	113	30.0	242	discarded		A132
654	O57-5	O57		08-28	16:30	Lisa	Growth	234C1-1	Qiagen miniprep kit	Plasmid	57	57	pSBX1A3-BBa_I13507	H2O	30.0	258	25.1	68	to be used further		L57#5
655	O129	O129		08-28	17:50	Caro	Growth	552C1	Qiagen miniprep kit	Plasmid	129	129	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	H2O	30.0		#DIV/0!				S129#3	76DB89, 76DB90	ok, ok
656	R196	R196		08-28	20:00	MaxW	Gel	214-2, 214-3	Gel extraction	Restriction	196		pSBX4C5-2Zv2Npu (BsaI)	H2O	30.0	15	23.2
657	R197	R197		08-28	20:30	MaxW	Restriction	197	PCR purification kit	Restriction	197		LambdaLys_BsaI_c-ext_BsaI (BsaI)	H2O	20.0	12	16.7
658	R198	R198		08-28	20:30	MaxW	Restriction	198	PCR purification kit	Restriction	198		ccdB_BsmBI-BsaI_BsaI-BsmBI (BsmBI)	H2O	20.0	12	15.2
659	O129-2	O129		28-08	21:30	Caro	Growth	552C2	Qiagen miniprep kit	Plasmid	129	129	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	H2O	30.0		0	243	discarded		S129#3
660	O129-3	O129		28-08	21:30	Caro	Growth	552C3	Qiagen miniprep kit	Plasmid	129	129	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	H2O	30.0		0	244	discarded		S129#3
661	O129-4	O129		28-08	21:30	Caro	Growth	552C4	Qiagen miniprep kit	Plasmid	129	129	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	H2O	30.0		0		discarded		S129#3
662	O129-5	O129		28-08	21:30	Caro	Growth	552C5	Qiagen miniprep kit	Plasmid	129	129	pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC	H2O	30.0		0		discarded		S129#3
663	R206	R206		28-08	21:45	Lisa	Restriction	206	PCR purification kit	Restriction	206		SspDnaBC_M+H6_N->A+SGG (X/P)	H2O	30.0	21	20.0
664	R207	R207		28-08	21:45	Lisa	Restriction	207	PCR purification kit	Restriction	207		SspDnaBC_M-noH6_N->A+SGG (X/P)	H2O	30.0	25	24.4
665	R205	R205		29-09	2:00	Lisa	Gel	215-2, 215-3	Gel extraction	Restriction	205		pSB1C3-BBa_K741002 (X/P)	H2O	30.0	17	8.0
666	O111-3	O111		08-29	12:30	MaxW	Growth	501C1-1	Qiagen miniprep kit	Plasmid	111	111	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	H2O	50.0	126	38.0				G111#2	76BD93, 76BD94	ok, ok
667	O111-4	O111		08-29	12:30	MaxW	Growth	501C2-1	Qiagen miniprep kit	Plasmid	111	111	pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)	H2O	50.0	110	41.0				G111#2	76BD95, 76BD96	ok, ok
668	O110-4	O110		08-29	12:30	MaxW	Growth	476C3-1	Qiagen miniprep kit	Plasmid	110	110	pSBX1K3-2Zv2Npu:(LambdaLys-H6)	H2O	50.0	94	34.7				G110#2	76BD97, 76BD98	ok, ok
669	O137	O137		08-29	18:00	Jakob	Growth	564C6	Qiagen miniprep kit	Plasmid	137	137	pSBX4C5-1Gate-NpuDnaE(N)-H6	H2O	30.0	480	30.0		discarded		G137
670	O138	O138		08-29	6:00 PM	Jakob	Growth	565C6	Qiagen miniprep kit	Plasmid	138	138	pSBX4C5-H6-1GateNpuDnaE(C)	H2O	30.0	780	30.0		discarded		G138
671	P176-2	P176		08-27	12:00	Jakob	Gel	212-2-1, 212-3-1	Gel extraction	PP	176		BsmBI-ccdB-BsmBI_pSBX4C5_BsaI-mRFP-BsaI	H2O	30.0	18	30.0
672	P177-2	P177		08-27	12:00	Jakob	Gel	212-4-1, 212-5-1	Gel extraction	PP	177		BsaI-mRFP-BsaI_pSBX4C5_BsmBI-ccdB-BsmBI	H2O	30.0	24	30.0
673	P173-2	P173		08-30	16:30	MaxW	Gel	223-2, 223-3, 223-4, 223-5	Gel extraction	PP	173		LambdaLys_BsaI_c-ext_BsaI	H2O	30.0	239	25.8
674	R208	R208		08-30	20:40	Caro	Gel	224-2, 224-3	Gel extraction	Restriction	208		pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (X/P)	H2O	30.0	8	14.0
675	R209	R209		08-30	20:40	Caro	Restriction	209	PCR purification kit	Restriction	209		pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)	H2O	30.0	14	25.2
676	O113-3	O113		08-30	22:15	MaxW	Growth	562C1	Qiagen miniprep kit	Plasmid	113	113	pSBX1K3-2Zv2Npu:Xyla_circ_noHis	H2O	50.0	150	40.0	245			G113#3	76DC23, 76DC24	ok, ok
677	O113-4	O113		08-30	22:15	MaxW	Growth	562C4	Qiagen miniprep kit	Plasmid	113	113	pSBX1K3-2Zv2Npu:Xyla_circ_noHis	H2O	50.0	150	36.7	246			G113#3	76DB87, 76DB88	ok, ok
678	R214	R214		08-30	22:15	Anna	Gel	226-2, 226-3	Gel extraction	Restriction	214		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (E/P)	EB	30.0	8	30.0
679	R215	R215		08-30	22:15	Anna	Gel	226-4	Gel extraction	Restriction	215		pSBX4A5-BBa_I13507 (E/P)	EB	30.0	5	4.5
680	R210	R210		08-30	22:45	MaxW	Gel	225-2, 225-3	Gel extraction	Restriction	210		pSBX4C5-2Zv2Npu (BsaI)	H2O	30.0	53	23.1
681	R211	R211		08-30	22:45	MaxW	Gel	225-4, 225-5	Gel extraction	Restriction	211		pSBX1K3-2Zv2Npu (BsaI)	H2O	30.0	27	21.8
682	R212	R212		08-30	22:45	MaxW	Restriction	212	PCR purification kit	Restriction	212		LambdaLys_BsaI_c-ext_BsaI (BsaI)	H2O	20.0	40	12.3
683	R213	R213		08-30	22:45	MaxW	Restriction	213	PCR purification kit	Restriction	213		ccdB_BsmBI-BsaI_BsaI-BsmBI (BsmBI)	H2O	20.0	40	8.8
684	O137-2	O137		08-31	11:10	Jakob	Growth	564C1	Qiagen miniprep kit	Plasmid	137	137	pSBX4C5-1Gate-NpuDnaE(N)-H6	H2O	30.0		30.0		discarded		G137
685	O137-3	O137		08-31	11:10	Jakob	Growth	564C2	Qiagen miniprep kit	Plasmid	137	137	pSBX4C5-1Gate-NpuDnaE(N)-H6	H2O	30.0		30.0		discarded		G137
686	O138-2	O138		08-31	11:10	Jakob	Growth	565C1	Qiagen miniprep kit	Plasmid	138	138	pSBX4C5-H6-1GateNpuDnaE(C)	H2O	30.0		30.0		discarded		G138
687	O138-3	O138		08-31	11:10	Jakob	Growth	565C2	Qiagen miniprep kit	Plasmid	138	138	pSBX4C5-H6-1GateNpuDnaE(C)	H2O	30.0		30.0		discarded		G138
688	O108-3	O108			todo	Caro	Growth	530C1-1	Qiagen miniprep kit	Plasmid	108	108	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6				0.0				A108
689	R217	R217		09-01	12:00	Lisa	Gel	238-2, 238-3	Gel extraction	Restriction	217		pSB1C3-BBa_K741002 (X/P)	H2O	30.0	30	15.9
690	R216	R216		09-01	14:00	Caro	Restriction	216	PCR purification kit	Restriction	216		pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (S/P)	H2O	30.0	14	25.3
691	O140	O140		09-01	15:00	Lisa	Growth	567C2	Qiagen miniprep kit	Plasmid	139	139	pSB1C3-T7RBS_+His_SspDnaBC_Ext	H2O	50.0	262	42.7	273	whoop how did that happen?		S140	76DB99, 76DC00	it has a his tag (the two plates were mixed up) -> juhu! intein protease ready,
692	O140-2	O140		09-01	15:00	Lisa	Growth	567C3	Qiagen miniprep kit	Plasmid	139	139	pSB1C3-T7RBS_+His_SspDnaBC_Ext	H2O	50.0	240	50.0	274	discarded		S140
693	R218	R218		09-01	13:00	Lisa	Gel	237-2, 237-3	Gel extraction	Restriction	218		SspDnaBC_M+H6_N->A+SGG (X/P)	H2O	30.0	22	25.9
694	R219	R219		09-01	13:00	Lisa	Gel	237-4, 237-5	Gel extraction	Restriction	219		SspDnaBC_M-noH6_N->A+SGG (X/P)	H2O	30.0	19	21.8
695	O131-3	O131		09-01	15:30	MaxW	Growth	583C1	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	50.0	269	50.0	249	discarded		L131#2
696	O131-4	O131		09-01	15:30	MaxW	Growth	583C2	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	50.0	298	46.8	250			L131#2	76DC01, 76DC02	ok, ok
697	O131-5	O131		09-01	15:30	MaxW	Growth	583C4	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	50.0	336	50.0	251	discarded		L131#2
698	O131-6	O131		09-01	15:30	MaxW	Growth	583C6	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	50.0	386	50.0	252	discarded		L131#2
699	O141	O141		09-01	15:30	MaxW	Growth	584C1	Qiagen miniprep kit	Plasmid	141	141	pSBX1K3-2Zv2Npu:LaLys+ccdb	H2O	50.0	108	50.0	253	discarded		L141
700	O141-2	O141		09-01	15:30	MaxW	Growth	584C3	Qiagen miniprep kit	Plasmid	141	141	pSBX1K3-2Zv2Npu:LaLys+ccdb	H2O	50.0	353	46.8	254			L141	76DC03, 76DC04	ok, ok
701	O141-3	O141		09-01	15:30	MaxW	Growth	584C5	Qiagen miniprep kit	Plasmid	141	141	pSBX1K3-2Zv2Npu:LaLys+ccdb	H2O	50.0	302	50.0	255	discarded		L141
702	O131-7	O131		09-01	15:30	MaxW	Growth	585C5	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	50.0	351	46.8	257			G131#2	76DC05, 76DC06	ok, ok
703	O131-8	O131		09-01	15:30	MaxW	Growth	585C6	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	50.0	367	46.8	258			G131#2	76DC07, 76DC08	ok, ok
704	O131-9	O131		09-01	15:30	MaxW	Growth	585C7	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	50.0	480	50.0	259	discarded		G131#2
705	O131-10	O131		09-01	15:30	MaxW	Growth	585C9	Qiagen miniprep kit	Plasmid	131	131	pSBX4C5-2Zv2Npu:LaLys+ccdb	H2O	50.0	410	50.0	260	discarded		G131#2
706	O141-4	O141		09-01	15:30	MaxW	Growth	586C5	Qiagen miniprep kit	Plasmid	141	141	pSBX1K3-2Zv2Npu:LaLys+ccdb	H2O	50.0	160	50.0	261	discarded		G141
707	O141-5	O141		09-01	15:30	MaxW	Growth	586C8	Qiagen miniprep kit	Plasmid	141	141	pSBX1K3-2Zv2Npu:LaLys+ccdb	H2O	50.0	141	50.0	262	discarded		G141
708	O141-6	O141		09-01	15:30	MaxW	Growth	586C9	Qiagen miniprep kit	Plasmid	141	141	pSBX1K3-2Zv2Npu:LaLys+ccdb	H2O	50.0	330	46.8	263	discarded		G141	76DC09, 76DC10	mutation in ccdb (R->H), mutation in ccdb (R->H)
709	R208-2	R208		08-27	15:00	Caro	Gel	210-2,210-3	Gel extraction	Restriction	208		pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (X/P)	H2O		10	0.0
710	O108-4	O108		09-01	18:30	Jakob	Growth	530C1-1	Qiagen miniprep kit	Plasmid	108	108	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	H2O	30.0	500	27.2				A108	76DC13, 76DC14	ok, ok
711	O137-4	O137		09-01	18:30	Jakob	Growth	564C3	Qiagen miniprep kit	Plasmid	137	137	pSBX4C5-1Gate-NpuDnaE(N)-H6	H2O	30.0	700	27.3	264			G137	76DC17, 76DC18	ok, ok
712	O138-4	O138		09-01	18:30	Jakob	Growth	565C3	Qiagen miniprep kit	Plasmid	138	138	pSBX4C5-H6-1GateNpuDnaE(C)	H2O	30.0	520	26.3	265			G138	76DC20, 76DC21	ok, ok
713	O103-5	O103		09-01	23:45	MaxW	Purification	O103-4	Dilution	Plasmid	103	103	pSBX1K3-2Zv2Npu	H2O	87.0	1	85.0
714	P197	P197		09-01	12:00	Jakob	Gel	241-2-1, 241-3-1, 241-4-1	Gel extraction	PP	197		ccdB-pSBX4C5-mRFP(N)_	H2O	30.0	16	30.0
715	P198	P198		09-01	12:00	Jakob	Gel	242-2-1, 242-3-1	Gel extraction	PP	198		mRFP-pSBX4C5-ccdB-(C)_	H2O	30.0	18	30.0
716	0-4	0				Caro					0		?	H2O	30.0		30.0
717	0-5	0				Caro					0		?	H2O	30.0		30.0
718	O133	O133		09-02	16:00	Anna	Growth	595C1	Qiagen miniprep kit	Plasmid	133	133	pSB1AK8-BBa_I712074-BBa_I13507	H2O	30.0	4	0	266	discarded		S133
719	O133-2	O133		09-02	16:00	Anna	Growth	595C1	Qiagen miniprep kit	Plasmid	133	133	pSB1AK8-BBa_I712074-BBa_I13507	H2O	30.0	47	30.0	266			S133
720	O133-3	O133		09-02	16:00	Anna	Growth	595C2	Qiagen miniprep kit	Plasmid	133	133	pSB1AK8-BBa_I712074-BBa_I13507	H2O	30.0	68	30.0	267			S133
721	O133-4	O133		09-02	16:00	Anna	Growth	595C3	Qiagen miniprep kit	Plasmid	133	133	pSB1AK8-BBa_I712074-BBa_I13507	H2O	30.0	72	30.0	268			S133
722	O137-5	O137		09-02	17:00	Caro	Purification	O137-4	Dilution	Plasmid	137	137	pSBX4C5-1Gate-NpuDnaE(N)-H6		35.0	20	35.0
723	O138-5	O138		09-02	17:00	Caro	Purification	O138-4	Dilution	Plasmid	138	138	pSBX4C5-H6-1GateNpuDnaE(C)		26.0	20	26.0
724	P97-2	P97		09-02	17:00	Caro	Purification	P97	Dilution	PP	97		mRFPn_1-154		7.00	20	7.00
725	P99-2	P99		09-02	17:00	Caro	Purification	P99	Dilution	PP	99		mRFPc_155-225		8.00	20	8.00
726	P98-2	P98		09-02	17:00	Caro	Purification	P98	Dilution	PP	98		mRFP_1-168		6.00	20	6.00
727	P100-2	P100		09-02	17:00	Caro	Purification	P100	Dilution	PP	100		mRFPc_169-225		8.00	20	8.00
728	P101-2	P101		09-02	17:00	Caro	Purification	P101	Dilution	PP	101		RenLucN_1-229_H6_GATG_BsaI_KGG_TGCT_BsaI		8.00	20	8.00
729	P102-2	P102		09-02	17:00	Caro	Purification	P102	Dilution	PP	102		RenLucC_230-311_NWC_CAAC_BsaI_H6_TAAT_BsaI		8.50	20	8.50
730	P103-2	P103		09-02	17:00	Caro	Purification	P103	Dilution	PP	103		FireLucN_1-437_H6_GATG_BsaI_KGG_TGCT_BsaI		4.50	20	4.50
731	P104-2	P104		09-02	17:00	Caro	Purification	P104	Dilution	PP	104		FireLucC_438-550_NWC_CAAC_BsaI_H6_TAAT_BsaI		8.00	20	8.00
732	P105-2	P105		09-02	17:00	Caro	Purification	P105	Dilution	PP	105		mCherryN_1-168_H6_GATG_BsaI_KGG_TGCT_BsaI		2.50	20	2.50
733	P106-2	P106		09-02	17:00	Caro	Purification	P106	Dilution	PP	106		mCherryC_169-236_NWC_CAAC_BsaI_H6_TAAT_BsaI		6.00	20	6.00
734	P107-2	P107		09-02	17:00	Caro	Purification	P107	Dilution	PP	107		GFPn_1-64_H6_BsaI		5.00	20	5.00
735	P109-2	P109		09-02	17:00	Caro	Purification	P109	Dilution	PP	109		GFPc_65-238_NWC_CAAC_BsaI_H6_TAAT_BsaI		6.00	20	6.00
736	P108-2	P108		09-02	17:00	Caro	Purification	P108	Dilution	PP	108		GFPn_1-157_H6_BsaI		8.00	20	8.00
737	P110-2	P110		09-02	17:00	Caro	Purification	P110	Dilution	PP	110		GFPc_158-238_NWC_CAAC_BsaI_H6_TAAT_BsaI		5.00	20	5.00
738	R221	R221		09-02	19:30	Lisa	Gel	246-2, 246-3	Gel extraction	Restriction	221		pSB1C3-BBa_K808000 (E/S)	H2O	30.0	8	1.4
739	R220	R220		09-02	19:30	Lisa	Restriction	220	PCR purification kit	Restriction	220		pSB1C3-T7RBS_+His_SspDnaBC_Ext (E/X)	H2O	30.0	6	25.8
740	P199	P199		09-02	21:30	MaxW	Gel	247-2, 247-3	Gel extraction	PP	199		p2Zv2Npu_Smt3_flag_Smt3	H2O	30.0	34	30.0
741	P200	P200		09-02	21:30	MaxW	Gel	247-4, 247-5	Gel extraction	PP	200		Smt3_p2Zv2Npu_flag_p2Zv2Npu	H2O	30.0	57	30.0
742	O130	O130		09-03	0:30	Caro	Growth	601C1	Qiagen miniprep kit	Plasmid	130	130	pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG	H2O	50.0	60	50.0	275			S130#5	BI2119, BI2120	,
743	O130-2	O130		09-03	0:30	Caro	Growth	602C1	Qiagen miniprep kit	Plasmid	130	130	pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG	H2O	50.0	74	50.0	276	discarded		S130#5
744	P160	P160		09-03	22:00	Lisa	Gel	253-2, 253-3, 253-4	Gel extraction	PP	160		pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI	H2O	30.0	33	30.0
745	P217	P217		09-03	21:35	Silvan	Gel	252-2-2.7kb,252-3-2.7kb	Gel extraction	PP	217		BsaI=GGGT-G4-DNMT1-731-1602-Flag-CTTC=BsaI	EB	30.0	28	2.7
746	P218	P218		09-03	21:35	Silvan	Gel	252-4-1.1kb,252-5-1.1kb	Gel extraction	PP	218		CGCA=BsaI-PlacI-mRFP-BsaI=CTTC	EB	30.0	102	20.2
747	R222	R222		09-03	22:15	Silvan	Restriction	222	PCR purification kit	Restriction	222		BsaI=GGGT-G4-DNMT1-731-1602-Flag-CTTC=BsaI (BsaI)	EB	30.0	11	30.0
748	R223	R223		09-03	22:15	Silvan	Restriction	223	PCR purification kit	Restriction	223		CGCA=BsaI-PlacI-mRFP-BsaI=CTTC (BsaI)	EB	30.0	26	30.0
749	R224	R224		09-03	22:15	Silvan	Restriction	224	PCR purification kit	Restriction	224		mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaI (BsaI)	EB	30.0	15	30.0
750	R225	R225		09-03	23:45	Silvan	Gel	261-2-2.5kb	Gel extraction	Restriction	225		pSBX1K3-BBa_I13507 (E/P)	EB	15.0	15	15.0
751	R226	R226		09-03	23:45	Silvan	Gel	261-3-1.1kb,261-4-1.1kb	Gel extraction	Restriction	226		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (E/P)	EB	15.0	10	15.0
752	O59-12	O59		09-04	16:40	Silvan	Growth	236C1-1	Qiagen maxiprep kit	Plasmid	59	59	pSBX1K3-BBa_I13507	EB	400.0	823	384.6				L59#5
753	O160	O160		09-04	16:40	MaxW	Growth	610C1	Qiagen miniprep kit	Plasmid	160	160	pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu	H2O	50.0	299	50.0	283	discarded		A160	BI2115, BI2116	mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R), mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R)
754	O160-2	O160		09-04	16:40	MaxW	Growth	610C2	Qiagen miniprep kit	Plasmid	160	160	pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu	H2O	50.0	347	50.0	284			A160	BI2117, BI2118	bad sequencing, auch eine in mrfp
755	O160-3	O160		09-04	16:40	MaxW	Growth	610C3	Qiagen miniprep kit	Plasmid	160	160	pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu	H2O	50.0	372	50.0	285	discarded		A160	BI2147, BI2148	insertion in smt3, silent in mfrp
756	O160-4	O160		09-04	16:40	MaxW	Growth	610C4	Qiagen miniprep kit	Plasmid	160	160	pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu	H2O	50.0	387	48.7	286			A160	BI2149, BI2150, AF9230	ok, ok (silent in mrfp),
757	O159	O159		09-04	16:40	Lisa	Growth	609C1	Qiagen miniprep kit	Plasmid	159	159	pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext	H2O	50.0	136	50.0	289	discarded		S159
758	O159-2	O159		09-04	16:40	Lisa	Growth	609C3	Qiagen miniprep kit	Plasmid	159	159	pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext	H2O	50.0	325	50.0	290	discarded		S159
759	O159-3	O159		09-04	16:40	Lisa	Growth	609C5	Qiagen miniprep kit	Plasmid	159	159	pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext	H2O	50.0	188	45.2	291			S159	76DB85, 76DB86	ok, ok, juhu, one part of intein protease ready, you should ring bell
760	R228	R228		09-04	18:45	Silvan	Gel	263-2-x	Gel extraction	Restriction	228		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (X/P)	EB	15.0	17	3.7
761	O145	O145		09-04	18:20	Caro	Growth	612C1	Qiagen miniprep kit	Plasmid	145	145	pSBX4C5-H6-mRFPn1-154-NpuN-H6	H2O		600	150	292			G145	BI2121, BI2122	,
762	O145-2	O145		09-04	18:20	Caro	Growth	612C2	Qiagen miniprep kit	Plasmid	145	145	pSBX4C5-H6-mRFPn1-154-NpuN-H6	H2O		600	148	293			G145
763	O146	O146		09-04	18:20	Caro	Growth	613C2	Qiagen miniprep kit	Plasmid	146	146	pSBX4C5-H6-NpuC-mRFP155-225-H6	H2O		600	208	294			G146	BI2123, BI2124	,
764	O146-2	O146		09-04	18:20	Caro	Growth	613C3	Qiagen miniprep kit	Plasmid	146	146	pSBX4C5-H6-NpuC-mRFP155-225-H6	H2O		600	115	295			G146
765	O147	O147		09-04	18:20	Caro	Growth	614C1	Qiagen miniprep kit	Plasmid	147	147	pSBX4C5-H6-mRFP1-168-NpuN-H6	H2O		600	104	296			G147	BI2125, BI2126	,
766	O147-2	O147		09-04	18:20	Caro	Growth	614C2	Qiagen miniprep kit	Plasmid	147	147	pSBX4C5-H6-mRFP1-168-NpuN-H6	H2O		600	117	297			G147
767	O148	O148		09-04	18:20	Caro	Growth	615C2	Qiagen miniprep kit	Plasmid	148	148	pSBX4C5-H6-NpuC-mRFP169-225-H6	H2O		600	29	298			G148	BI2127, BI2128	,
768	O148-2	O148		09-04	18:20	Caro	Growth	615C3	Qiagen miniprep kit	Plasmid	148	148	pSBX4C5-H6-NpuC-mRFP169-225-H6	H2O		600	56	299			G148
769	O149	O149		09-04	18:20	Caro	Growth	616C2	Qiagen miniprep kit	Plasmid	149	149	pSBX4C5-H6-RenLucN-NpuN-H6	H2O		600	19	300			G149	BI2129, BI2130	,
770	O149-2	O149		09-04	18:20	Caro	Growth	616C3	Qiagen miniprep kit	Plasmid	149	149	pSBX4C5-H6-RenLucN-NpuN-H6	H2O		600	69	301			G149
771	O150	O150		09-04	18:20	Caro	Growth	617C2	Qiagen miniprep kit	Plasmid	150	150	pSBX4C5-H6-NpuC-RenLucC-H6	H2O		600	34	302			G150	BI2131, BI2132	,
772	O150-2	O150		09-04	18:20	Caro	Growth	617C4	Qiagen miniprep kit	Plasmid	150	150	pSBX4C5-H6-NpuC-RenLucC-H6	H2O		600	64	303			G150
773	O151	O151		09-04	18:20	Caro	Growth	618C2	Qiagen miniprep kit	Plasmid	151	151	pSBX4C5-H6-FireLucN-NpuN-H6	H2O		600	111	304			G151	BI2133, BI2134	,
774	O151-2	O151		09-04	18:20	Caro	Growth	618C3	Qiagen miniprep kit	Plasmid	151	151	pSBX4C5-H6-FireLucN-NpuN-H6	H2O		600	70	305			G151
775	O152	O152		09-04	18:20	Caro	Growth	619C2	Qiagen miniprep kit	Plasmid	152	152	pSBX4C5-H6-NpuC-FireLucC-H6	H2O		600	1	306			G152	BI2135, BI2136	,
776	O152-2	O152		09-04	18:20	Caro	Growth	619C3	Qiagen miniprep kit	Plasmid	152	152	pSBX4C5-H6-NpuC-FireLucC-H6	H2O		600	1	307			G152
777	O153	O153		09-04	18:20	Caro	Growth	620C2	Qiagen miniprep kit	Plasmid	153	153	pSBX4C5-H6-mCherryN-NpuN-H6	H2O		600	0	308			G153	BI2137, BI2138	,
778	O153-2	O153		09-04	18:20	Caro	Growth	620C3	Qiagen miniprep kit	Plasmid	153	153	pSBX4C5-H6-mCherryN-NpuN-H6	H2O		600	23	309			G153
779	O154	O154		09-04	18:20	Caro	Growth	621C2	Qiagen miniprep kit	Plasmid	154	154	pSBX4C5-H6-NpuC-mCherryC-H6	H2O		600	0	310			G154	BI2139, BI2140	,
780	O154-2	O154		09-04	18:20	Caro	Growth	621C3	Qiagen miniprep kit	Plasmid	154	154	pSBX4C5-H6-NpuC-mCherryC-H6	H2O		600	0	311			G154
781	O155	O155		09-04	18:20	Caro	Growth	622C1	Qiagen miniprep kit	Plasmid	155	155	pSBX4C5-H6-GFPn1-64-NpuN-H6	H2O		600	0	312			G155	BI2141, BI2142	,
782	O155-2	O155		09-04	18:20	Caro	Growth	622C2	Qiagen miniprep kit	Plasmid	155	155	pSBX4C5-H6-GFPn1-64-NpuN-H6	H2O		600	0	313			G155
783	O157	O157		09-04	18:20	Caro	Growth	624C2	Qiagen miniprep kit	Plasmid	157	157	pSBX4C5-H6-GFPn1-157-NpuN-H6	H2O		600	0	314			G157	BI2143, BI2144	,
784	O157-2	O157		09-04	18:20	Caro	Growth	624C2	Qiagen miniprep kit	Plasmid	157	157	pSBX4C5-H6-GFPn1-157-NpuN-H6	H2O		600	0	314			G157
785	O158	O158		09-04	18:20	Caro	Growth	625C1	Qiagen miniprep kit	Plasmid	158	158	pSBX4C5-H6-GFPc158-238-NpuC-H6	H2O		600	0	316			G158
786	O158-2	O158		09-04	18:20	Caro	Growth	625C2	Qiagen miniprep kit	Plasmid	158	158	pSBX4C5-H6-GFPc158-238-NpuC-H6	H2O		600	0	317			G158
787	R227	R227		09-04	19:00	Silvan	Restriction	227	PCR purification kit	Restriction	227		pSBX1K3-T7RBS_BBv2 (S/P)	EB	15.0	29	10.7
788	P180-2	P180		09-05	20:00	MaxW	Purification	P180	Dilution	PP	180		Ssp DnaX-S11N	H2O	50.0	1	46.0
789	P182-2	P182		09-05	20:00	MaxW	Purification	P182	Dilution	PP	182		Ssp GyrB-S11N	H2O	50.0	1	46.0
790	P184-2	P184		09-05	20:00	MaxW	Purification	P184	Dilution	PP	184		Ter DnaE3-S11N	H2O	50.0	1	46.0
791	P197-2	P197		09-06	1:30	MaxW	Gel	265-2, 265-3	Gel extraction	PP	197		ccdB-pSBX4C5-mRFP(N)_	H2O	30.0	65	30.0
792	P197-3	P197		09-06	1:30	MaxW	Gel	265-4, 265-4	Gel extraction	PP	197		ccdB-pSBX4C5-mRFP(N)_	H2O	30.0	64	30.0
793	P197-4	P197		09-06	1:30	MaxW	Gel	265-6, 265-7	Gel extraction	PP	197		ccdB-pSBX4C5-mRFP(N)_	H2O	30.0	62	30.0
794	P198-2	P198		09-06	1:30	MaxW	Gel	265-8, 265-9	Gel extraction	PP	198		mRFP-pSBX4C5-ccdB-(C)_	H2O	30.0	74	30.0
795	P198-3	P198		09-06	1:30	MaxW	Gel	265-10, 265-11	Gel extraction	PP	198		mRFP-pSBX4C5-ccdB-(C)_	H2O	30.0	50	30.0
796	P198-4	P198		09-06	1:30	MaxW	Gel	265-12, 265-13	Gel extraction	PP	198		mRFP-pSBX4C5-ccdB-(C)_	H2O	30.0	51	30.0
797	P223	P223		09-06	1:30	MaxW	Gel	267-2, 267-3	Gel extraction	PP	223		Ssp DnaX-S11N_BsaI_mRFP_BsmBI_ccdB	H2O	20.0	188	20.0
798	P224	P224		09-06	1:30	MaxW	Gel	267-4, 267-5	Gel extraction	PP	224		Ssp DnaX-S11N_C->G_BsaI_mRFP_BsmBI_ccdB	H2O	20.0	169	20.0
799	P225	P225		09-06	1:30	MaxW	Gel	267-6, 267-7	Gel extraction	PP	225		Ssp GyrB-S11N_BsaI_mRFP_BsmBI_ccdB	H2O	20.0	160	20.0
800	P226	P226		09-06	1:30	MaxW	Gel	267-8, 267-9	Gel extraction	PP	226		Ssp GyrB-S11N_C->G_BsaI_mRFP_BsmBI_ccdB	H2O	20.0	166	20.0
801	P227	P227		09-06	1:30	MaxW	Gel	267-10, 267-11	Gel extraction	PP	227		Ter DnaE3-S11N_BsaI_mRFP_BsmBI_ccdB	H2O	20.0	157	20.0
802	P228	P228		09-06	1:30	MaxW	Gel	267-12, 267-13	Gel extraction	PP	228		Ter DnaE3-S11N_C->G_BsaI_mRFP_BsmBI_ccdB	H2O	20.0	106	20.0
803	P231	P231		09-06	3:50	Silvan	Gel	268-8, 268-9	Gel extraction	PP	231		mRFPexp_Smt3-TEVc-BsaI_mockSortAc-BBsuffix	EB	30.0	146	30.0
804	P233	P233		09-06	3:50	Silvan	Gel	268-10,268-11	Gel extraction	PP	233		mRFPexp_H6-TEVc_mockSortAc-BBsuffix	EB	30.0	107	30.0
805	P235	P235		09-06	3:50	Silvan	Gel	268-12,268-13	Gel extraction	PP	235		mRFPexp_TEVc-BsaI_SortAc-H6-BBsuffix	EB	30.0	131	30.0
806	P236	P236		09-06	3:50	Silvan	Gel	268-15,268-16	Gel extraction	PP	236		eGFP_BsaI-G5RM_BsaI	EB	30.0	153	30.0
807	P237	P237		09-06	3:50	Silvan	Gel	268-17,268-18	Gel extraction	PP	237		ccdB_BsaI-BsmBI_BsaI-BsmBI	EB	30.0	159	30.0
808	P238	P238		09-06	3:50	Silvan	Gel	268-19,268-20	Gel extraction	PP	238		H6Smt3_BBprefix_TEVc-BsaI	EB	30.0	32	30.0
809	P239	P239		09-06	3:50	Silvan	Gel	268-21,268-22	Gel extraction	PP	239		Npu DnaEC_BsmBI_ccdB_SN->AG-BsaI-mRFP	EB	30.0	52	30.0
810	P240	P240		09-06	3:50	Silvan	Gel	268-23,268-24	Gel extraction	PP	240		NpuDnaEN_C->G-BsaI-mRFP_BsmBI_ccdB	EB	30.0	79	30.0
811	P221	P221		09-06	12:15	Jan	Gel	264-2-1	Gel extraction	PP	221	143	pSBX4C5-2GateNpuDnaEN C-G	H2O	10.0	29	10.0
812	P222	P222		09-06	12:15	Jan	Gel	264-3-1	Gel extraction	PP	222	144	pSBX4C5-2GateNpuDnaEC SN->AG	H2O	10.0	18	10.0
813	P241	P241		09-06	15:30	MaxW	Oligos	215,216	Mix	PP	241		Ssp DnaX-S11C_BsmBI_ccdB_BsaI_mRFP	H2O	200.0	19	200.0		not real
814	P242	P242		09-06	15:30	MaxW	Oligos	215,217	Mix	PP	242		Ssp DnaX-S11C_BsmBI_ccdB_HN->AG_BsaI_mRFP	H2O	200.0	19	200.0		not real
815	P243	P243		09-06	15:30	MaxW	Oligos	219,220	Mix	PP	243		Ssp GyrB-S11C_BsmBI_ccdB_BsaI_mRFP	H2O	200.0	19	200.0		not real
816	P244	P244		09-06	15:30	MaxW	Oligos	219,221	Mix	PP	244		Ssp GyrB-S11C_BsmBI_ccdB_SN->AG_BsaI_mRFP	H2O	200.0	19	200.0		not real
817	P245	P245		09-06	15:30	MaxW	Oligos	223,224	Mix	PP	245		Ter DnaE3-S11C_BsmBI_ccdB_BsaI_mRFP	H2O	200.0	19	200.0		not real
818	P246	P246		09-06	15:30	MaxW	Oligos	223,225	Mix	PP	246		Ter DnaE3-S11C_BsmBI_ccdB_SN->AG_BsaI_mRFP	H2O	200.0	19	200.0		not real
819	O12-5	O12		09-06	16:35	NilsH	Purification	O12	Dilution	Plasmid	12	12	pUC19	H2O	50.0	0	50.0
820	R229	R229		09-06	20:00	Anna	Gel	269-2	Gel extraction	Restriction	229		pSBX1K3-T7RBS_BBv2 (S/P)	H2O	30.0	35	27.1
821	R230	R230		09-06	20:00	Anna	Gel	269-3	Gel extraction	Restriction	230		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (X/P)	H2O	30.0	14	16.9
822	R231	R231		09-06	19:50	Lisa	Oligos	178,179	Annealing	Restriction	231		BamHI-MTS_bs_minD-KpnI-**_F (BamHI/K)	T4Ligase Buffer	100.0	29	96.1
823	O145-3	O145		09-07	11:00	Caro	Growth	612C1-1	Qiagen miniprep kit	Plasmid	145	145	pSBX4C5-H6-mRFPn1-154-NpuN-H6	H2O	30.0	265	24.3				G145	BI2151, BI2163	ok,
824	O146-3	O146		09-07	11:00	Caro	Growth	613C2-1	Qiagen miniprep kit	Plasmid	146	146	pSBX4C5-H6-NpuC-mRFP155-225-H6	H2O	30.0	330	20.9				G146	BI2152, BI2164	ok,
825	O147-3	O147		09-07	11:00	Caro	Growth	614C1-1	Qiagen miniprep kit	Plasmid	147	147	pSBX4C5-H6-mRFP1-168-NpuN-H6	H2O	30.0	369	27.3				G147	BI2153, BI2165	kok,
826	O148-3	O148		09-07	11:00	Caro	Growth	615C2-1	Qiagen miniprep kit	Plasmid	148	148	pSBX4C5-H6-NpuC-mRFP169-225-H6	H2O	30.0	250	18.0				G148	BI2154	ok
827	O149-3	O149		09-07	11:00	Caro	Growth	616C2-1	Qiagen miniprep kit	Plasmid	149	149	pSBX4C5-H6-RenLucN-NpuN-H6	H2O	30.0	171	24.2				G149	BI2155, BI2166	ok, one read bad, silent mutation in ligation renlucN to NpuN,
828	O150-3	O150		09-07	11:00	Caro	Growth	617C2-1	Qiagen miniprep kit	Plasmid	150	150	pSBX4C5-H6-NpuC-RenLucC-H6	H2O	30.0	188	14.0				G150	BI2156	ok, TAA insertion after His Tag
829	O151-3	O151		09-07	11:00	Caro	Growth	618C2-1	Qiagen miniprep kit	Plasmid	151	151	pSBX4C5-H6-FireLucN-NpuN-H6	H2O	30.0	162	23.8				G151	BI2157, BI2167	ok,
830	O152-3	O152		09-07	11:00	Caro	Growth	619C2-1	Qiagen miniprep kit	Plasmid	152	152	pSBX4C5-H6-NpuC-FireLucC-H6	H2O	30.0	326	20.8				G152	BI2158	ok
831	O153-3	O153		09-07	11:00	Caro	Growth	620C2-1	Qiagen miniprep kit	Plasmid	153	153	pSBX4C5-H6-mCherryN-NpuN-H6	H2O	30.0	169	21.1				G153	BI2159, BI2168	ok,
832	O154-3	O154		09-07	11:00	Caro	Growth	621C2-1	Qiagen miniprep kit	Plasmid	154	154	pSBX4C5-H6-NpuC-mCherryC-H6	H2O	30.0	535	22.7				G154	76DB91, 76DB92	ok, ok
833	O155-3	O155		09-07	11:00	Caro	Growth	622C1-1	Qiagen miniprep kit	Plasmid	155	155	pSBX4C5-H6-GFPn1-64-NpuN-H6	H2O	30.0	192	30.0				G155	BI2160, BI2169	ok,
834	O157-3	O157		09-07	11:00	Caro	Growth	624C2-2	Qiagen miniprep kit	Plasmid	157	157	pSBX4C5-H6-GFPn1-157-NpuN-H6	H2O	30.0	646	28.5				G157	BI2161, BI2170	ok,
835	O158-3	O158		09-07	12:00	Caro	Growth	625C1-1	Qiagen miniprep kit	Plasmid	158	158	pSBX4C5-H6-GFPc158-238-NpuC-H6	H2O	30.0	640	25.3				G158	BI2162	ok, one shift after "stem loop"
836	P230	P230		09-07	20:00	Silvan	Gel	275-2,275-3,275-4,275-5	Gel extraction	PP	230		pSB1C3_mockSortAc_H6-Smt3	EB	15.0	117	15.0
837	P232	P232		09-07	20:00	Silvan	Gel	275-6,275-7-275-8,275-9	Gel extraction	PP	232		pSB1C3_mockSortAc_H6-TEVc	EB	15.0	30	15.0
838	P234	P234		09-07	20:00	Silvan	Gel	275-10,275-11,275-12	Gel extraction	PP	234		pSB1C3_SortAc-H6_TEVc-BsaI	EB	15.0	58	15.0
839	O162	O162		09-08	11:00	Anna	Growth	666C2	Qiagen miniprep kit	Plasmid	162	162	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)	H2O	50.0	20	50.0				S162#4
840	O162-2	O162		09-08	11:00	Anna	Growth	665C3	Qiagen miniprep kit	Plasmid	162	162	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)	H2O	50.0	35	50.0				S162#3	BI2196, BI2197	,
841	O163	O163		09-08	14:30	MaxW	Growth	646C1	Qiagen miniprep kit	Plasmid	163	163	pSBX4C5-2GateSspDnaX-S11C	H2O	50.0	188	50.0	323	used for 1Gate		A163	BI2145, BI2171	ok, ok
842	O163-2	O163		09-08	14:30	MaxW	Growth	646C2	Qiagen miniprep kit	Plasmid	163	163	pSBX4C5-2GateSspDnaX-S11C	H2O+X	50+X	28	0	324	discarded		A163
843	O164	O164		09-08	14:30	MaxW	Growth	647C1	Qiagen miniprep kit	Plasmid	164	164	pSBX4C5-2GateSspDnaX-S11C HN->AG	H2O	50.0	270	50.0	326	used for 1Gate		A164	BI2172, BI2173	ok, ok
844	O164-2	O164		09-08	14:30	MaxW	Growth	647C2	Qiagen miniprep kit	Plasmid	164	164	pSBX4C5-2GateSspDnaX-S11C HN->AG	H2O	50.0	266	50.0	327	discarded		A164
845	O165	O165		09-08	14:30	MaxW	Growth	648C1	Qiagen miniprep kit	Plasmid	165	165	pSBX4C5-2GateSspDnaX-S11N	H2O	50.0	200	50.0	329			A165	BI2174, BI2175, BI1917	part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, ok
846	O165-2	O165		09-08	14:30	MaxW	Growth	648C2	Qiagen miniprep kit	Plasmid	165	165	pSBX4C5-2GateSspDnaX-S11N	H2O	50.0	175	50.0	330	discarded		A165
847	O166	O166		09-08	14:30	MaxW	Growth	649C1	Qiagen miniprep kit	Plasmid	166	166	pSBX4C5-2GateSspDnaX-S11N C->G	H2O+X	50+X	53	#VALUE!	332			A166	BI2176, BI2177, BI1918	part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, ok
848	O166-2	O166		09-08	14:30	MaxW	Growth	649C2	Qiagen miniprep kit	Plasmid	166	166	pSBX4C5-2GateSspDnaX-S11N C->G	H2O+X	50+X	60	#VALUE!	333	discarded		A166
849	O167	O167		09-08	14:30	MaxW	Growth	650C1	Qiagen miniprep kit	Plasmid	167	167	pSBX4C5-2GateSspGyrB-S11C	H2O	50.0	234	50.0	335			A167	BI2178, BI2179	ok, ok
850	O167-2	O167		09-08	14:30	MaxW	Growth	650C2	Qiagen miniprep kit	Plasmid	167	167	pSBX4C5-2GateSspGyrB-S11C	H2O	50.0	257	50.0	336	discarded		A167
851	O168	O168		09-08	14:30	MaxW	Growth	651C1	Qiagen miniprep kit	Plasmid	168	168	pSBX4C5-2GateSspGyrB-S11C SN->AG	H2O	50.0	235	50.0	338			A168	BI2180, BI2181	ok, ok
852	O168-2	O168		09-08	14:30	MaxW	Growth	651C2	Qiagen miniprep kit	Plasmid	168	168	pSBX4C5-2GateSspGyrB-S11C SN->AG	H2O	50.0	266	50.0	339	discarded		A168
853	O169	O169		09-08	14:30	MaxW	Growth	652C1	Qiagen miniprep kit	Plasmid	169	169	pSBX4C5-2GateSspGyrB-S11N	H2O	50.0	205	50.0	341			A169	BI2182, BI2183, BI1919	part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, mutation in ccdb c->a (?), ok, only intein sequenzed
854	O169-2	O169		09-08	14:30	MaxW	Growth	652C2	Qiagen miniprep kit	Plasmid	169	169	pSBX4C5-2GateSspGyrB-S11N	H2O	50.0	339	50.0	342			A169	BI2198, BI2199	ok, ok (mut in ccdb E87*)
855	O171	O171		09-08	14:30	MaxW	Growth	654C1	Qiagen miniprep kit	Plasmid	171	171	pSBX4C5-2GateTerDnaE3-S11C	H2O	50.0	241	50.0	344			A171	BI2184, BI2185	ok, ok
856	O171-2	O171		09-08	14:30	MaxW	Growth	654C2	Qiagen miniprep kit	Plasmid	171	171	pSBX4C5-2GateTerDnaE3-S11C	H2O	50.0	160	50.0	345	discarded		A171
857	O172	O172		09-08	14:30	MaxW	Growth	655C2	Qiagen miniprep kit	Plasmid	172	172	pSBX4C5-2GateTerDnaE3-S11C SN->AG	H2O	50.0	178	50.0	347			A172	BI2186, BI2187	ok, ok
858	O172-2	O172		09-08	14:30	MaxW	Growth	655C3	Qiagen miniprep kit	Plasmid	172	172	pSBX4C5-2GateTerDnaE3-S11C SN->AG	H2O	50.0	177	50.0	348	discarded		A172
859	O173	O173		09-08	14:30	MaxW	Growth	656C1	Qiagen miniprep kit	Plasmid	173	173	pSBX4C5-2GateTerDnaE3-S11N	H2O	50.0	73	50.0	349	discarded		A173	BI2188, BI2189	mutation in lacO-reg g->t, part of mrfp+intein not sequenced, part of mrfp+intein not sequenced
860	O173-2	O173		09-08	14:30	MaxW	Growth	656C2	Qiagen miniprep kit	Plasmid	173	173	pSBX4C5-2GateTerDnaE3-S11N	H2O	50.0	95	50.0	350			A173	BI2200, BI2201, BI1921	ok, ok, ok
861	O174	O174		09-08	14:30	MaxW	Growth	657C1	Qiagen miniprep kit	Plasmid	174	174	pSBX4C5-2GateTerDnaE3-S11N C->G	H2O	50.0	228	50.0	352	discarded		A174	BI2190, BI2191	no mutations, begins in ccdb --> not fully seq, mut. in ccdb ga->tc
862	O174-2	O174		09-08	14:30	MaxW	Growth	657C2	Qiagen miniprep kit	Plasmid	174	174	pSBX4C5-2GateTerDnaE3-S11N C->G	H2O	50.0	128	50.0	353			A174	BI2202, BI2203, BI1922	ok, ok, ok
863	O143	O143		09-08	14:30	MaxW	Growth	658C1	Qiagen miniprep kit	Plasmid	143	143	pSBX4C5-2GateNpuDnaE(N)*	H2O	50.0	190	50.0	355	discarded		A143	BI2192, BI2193	part of intein/mrfp not sequenced, part of intein/mrfp not sequenced
864	O143-2	O143		09-08	14:30	MaxW	Growth	658C2	Qiagen miniprep kit	Plasmid	143	143	pSBX4C5-2GateNpuDnaE(N)*	H2O	50.0	141	50.0	356			A143	BI2204, BI2205, BI1923	ok, ok, ok
865	O144	O144		09-08	14:30	MaxW	Growth	659C1	Qiagen miniprep kit	Plasmid	144	144	pSBX4C5-2GateNpuDnaE(C)*	H2O	50.0	207	50.0	358	discard		A144
866	O144-2	O144		09-08	14:30	MaxW	Growth	659C2	Qiagen miniprep kit	Plasmid	144	144	pSBX4C5-2GateNpuDnaE(C)*	H2O	50.0	181	48.0	359			A144	BI2206, BI2207	ok, ok
867	P264	P264		09-09	14:00	Lisa	PCR	1201	PCR purification kit	PP	264		pSBX1C3-Ter_KpnI_Ext_IntN_BamHI	H2O	50.0	85	28.2
868	P263	P263		09-09	13:00	Caro	PCR	1200	PCR purification kit	PP	263	180	sfGFP_T65C_	H20	30.0		30.0
869	R238	R238		09-09	15:00	Lisa	Restriction	238	PCR purification kit	Restriction	238		pSBX1C3-Ter_KpnI_Ext_IntN_BamHI (BamHI/K)	H2O	50.0	20	40.8
870	O112-5	O112		09-09	18:00	Consti	Purification	O112-2	Dilution	Plasmid	112	112	pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His	H2O	350.0	1	348.0
871	P138-4	P138		09-09	21:30	Consti	Gel	284-2, 284-3	Gel extraction	PP	138		Xyla_BB_RBS34_X_SP				0.0
872	P217-2	P217		09-09	23:00	Anna	PCR	1215	PCR purification kit	PP	217		BsaI=GGGT-G4-DNMT1-731-1602-Flag-CTTC=BsaI	H2O	20.0	33	20.0
873	P147-2	P147		09-09	23:00	Anna	PCR	1216	PCR purification kit	PP	147		mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaI	H2O	20.0	46	20.0
874	R239	R239		09-09	0:00	Consti	Restriction	239	PCR purification kit	Restriction	239		Xyla_BB_RBS34_X_SP (X/P)	H2O	20.0	20	14.6		conc. estimated, nanodrop measured 27.3
875	R240	R240		09-09	0:00	Consti	Gel	285-2, 285-3	Gel extraction	Restriction	240		pSBX1K3-BBa_I13507 (X/P)	H2O	30.0	14	20.4		conc. estimated, nanodrop measured 19.9
876	O170	O170		09-10	17:30	MaxW	Growth	684C6	Qiagen miniprep kit	Plasmid	170	170	pSBX4C5-2GateSspGyrB-S11N C->G	H2O	50.0	191	50.0	367			A170#2	BI2208, BI2209, BI1920	ok, ok, ok
877	O170-2	O170		09-10	17:30	MaxW	Growth	684C7	Qiagen miniprep kit	Plasmid	170	170	pSBX4C5-2GateSspGyrB-S11N C->G	H2O	50.0	199	50.0	368	discarded		A170#2
878	O170-3	O170		09-10	17:30	MaxW	Growth	684C8	Qiagen miniprep kit	Plasmid	170	170	pSBX4C5-2GateSspGyrB-S11N C->G	H2O	50.0	224	50.0	369	discarded		A170#2
879	O135	O135		09-10	17:30	MaxW	Growth	685C6	Qiagen miniprep kit	Plasmid	135	135	pSBX4C5-2GateGp41-1(N)	H2O	50.0	120	50.0	370		y	A135b#2	BI2210, BI2211, BI1924	ok, ok, ok
880	O135-2	O135		09-10	17:30	MaxW	Growth	685C7	Qiagen miniprep kit	Plasmid	135	135	pSBX4C5-2GateGp41-1(N)	H2O	50.0	173	0	371	discarded		A135b#2
881	O135-3	O135		09-10	17:30	MaxW	Growth	685C8	Qiagen miniprep kit	Plasmid	135	135	pSBX4C5-2GateGp41-1(N)	H2O	50.0	162	0	372	discarded		A135b#2
882	O136	O136		09-10	17:30	MaxW	Growth	686C6	Qiagen miniprep kit	Plasmid	136	136	pSBX4C5-2GateGp41-1(C)	H2O	50.0	166	50.0	373		y	A136b#2	BI2212, BI2213	ok, ok
883	O136-2	O136		09-10	17:30	MaxW	Growth	686C7	Qiagen miniprep kit	Plasmid	136	136	pSBX4C5-2GateGp41-1(C)	H2O	50.0	174	50.0	374	discarded		A136b#2
884	O136-3	O136		09-10	17:30	MaxW	Growth	686C8	Qiagen miniprep kit	Plasmid	136	136	pSBX4C5-2GateGp41-1(C)	H2O	50.0	130	50.0	375	discarded		A136b#2
885	O180	O180		09-10	18:40	Caro	Growth	688C1	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	H2O	30.0	236	30.0		discarded		P263	BI1915, BI1916	deletion in 1st Stop codon, deletion in 1st Stop codon
886	R242	R242		09-10	18:40	Caro	Gel	292-2,292-3	Gel extraction	Restriction	242		pSBX4C5-H6-NpuC-mRFP155-225-H6 (X/P)			17	-3.4
887	R244	R244		09-10	18:40	Caro	Gel	292-4,292-5	Gel extraction	Restriction	244		pSBX4C5-H6-NpuC-mRFP169-225-H6 (X/P)			12	-1.6
888	R246	R246		09-10	18:40	Caro	Gel	292-6,292-7	Gel extraction	Restriction	246		pSBX4C5-H6-NpuC-RenLucC-H6 (X/P)			28	-1.2
889	R248	R248		09-10	18:40	Caro	Gel	292-9,292-10	Gel extraction	Restriction	248		pSBX4C5-H6-NpuC-FireLucC-H6 (X/P)			8	-4.9
890	R250	R250		09-10	18:40	Caro	Gel	292-11, 292-12	Gel extraction	Restriction	250		pSBX4C5-H6-NpuC-mCherryC-H6 (X/P)			10	-2.7
891	R252	R252		09-10	18:40	Caro	Gel	292-13,292-14	Gel extraction	Restriction	252		pSBX4C5-H6-GFPc158-238-NpuC-H6 (X/P)			15	-2.1
892	R241	R241		09-10	18:40	Caro	Gel	287-2,287-3	Gel extraction	Restriction	241		pSBX4C5-H6-mRFPn1-154-NpuN-H6 (S/P)			14	-9.6
893	R243	R243		09-10	18:40	Caro	Gel	287-4,287-5	Gel extraction	Restriction	243		pSBX4C5-H6-mRFP1-168-NpuN-H6 (S/P)			7	-15.4
894	R245	R245		09-10	18:40	Caro	Gel	287-6,287-7	Gel extraction	Restriction	245		pSBX4C5-H6-RenLucN-NpuN-H6 (S/P)			14	-10.8
895	R247	R247		09-10	18:40	Caro	Gel	287-9, 287-10	Gel extraction	Restriction	247		pSBX4C5-H6-FireLucN-NpuN-H6 (S/P)			19	-8.1
896	R249	R249		09-10	18:40	Caro	Gel	287-11,287-12	Gel extraction	Restriction	249		pSBX4C5-H6-mCherryN-NpuN-H6 (S/P)			13	-10.3
897	R251	R251		09-10	18:40	Caro	Gel	287-13, 287-14	Gel extraction	Restriction	251		pSBX4C5-H6-GFPn1-157-NpuN-H6 (S/P)			12	-10.9
898	O102-8	O102		09-10	22:00	MaxW	Purification	O102-3	Dilution	Plasmid	102	102	pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3	H2O	150.0	1	148.0
899	R259	R259		09-10	22:45	MaxW	Oligos	240,241	Annealing	Restriction	259		H6+exteins-insert	H2O	100.0	3	100.0
900	R260	R260		09-10	22:45	MaxW	Oligos	200,201	Annealing	Restriction	260		flex-linker	H2O	100.0	2	100.0
901	P147-3	P147		09-11	0:30	Silvan	Gel	294-2	Gel extraction	PP	147		mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaI	EB			0.0
902	P268	P268		09-11	1:30	MaxW	Gel	295-2, 295-3	Gel extraction	PP	268		LiLaLy_BsaI_IasB	H2O	20.0	41	20.0
903	P217-3	P217		09-11	1:30	Anna	Gel	291-2, 291-3	Gel extraction	PP	217		BsaI=GGGT-G4-DNMT1-731-1602-Flag-CTTC=BsaI	H2O	20.0	96	20.0
904	P147-4	P147		09-11	1:30	Anna	Gel	294-2	Gel extraction	PP	147		mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaI	H2O	20.0	27	20.0
905	P264-2	P264		09-11	10:30	Lisa	Gel	296-2, 296-3, 296-4	Gel extraction	PP	264		pSBX1C3-Ter_KpnI_Ext_IntN_BamHI	H2O	50.0	31	-15.6
906	R261	R261		09-11	13:00	Lisa	Restriction	261	PCR purification kit	Restriction	261		pSBX1C3-Ter_KpnI_Ext_IntN_BamHI (BamHI/K)	H2O	30.0	20	13.9
907	O181	O181		09-11	16:00	Consti	Growth	692C1	Qiagen miniprep kit	Plasmid	181	181	pSBX1K3-Xylanase	H2O	30.0	38	16.9	376			S181	BI1947	ok
908	O181-2	O181		09-11	16:00	Consti	Growth	692C2	Qiagen miniprep kit	Plasmid	181	181	pSBX1K3-Xylanase	H2O	30.0	32	30.0	377	discarded		S181
909	O181-3	O181		09-11	16:00	Consti	Growth	692C3	Qiagen miniprep kit	Plasmid	181	181	pSBX1K3-Xylanase	H2O	30.0	40	30.0	378			S181	BI12214	ok
910	O181-4	O181		09-11	16:00	Consti	Growth	692C4	Qiagen miniprep kit	Plasmid	181	181	pSBX1K3-Xylanase	H2O	30.0	38	0	379	discarded		S181
911	O181-5	O181		09-11	16:00	Consti	Growth	692C5	Qiagen miniprep kit	Plasmid	181	181	pSBX1K3-Xylanase	H2O	30.0	40	0	380	discarded		S181
912	O125-10	O125		09-11	18:30	Silvan	Growth	544C8-1	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	83	50.0	381			L125#2	BI1925, BI1932	,
913	O178	O178		09-11	18:30	Silvan	Growth	676C1	Qiagen miniprep kit	Plasmid	178	178	pSB1C3-H6-0ZSortA	EB	50.0	259	44.2	382			A178	BI1929, BI1936, AG3767	ok, no result, ok
914	O178-2	O178		09-11	18:30	Silvan	Growth	676C2	Qiagen miniprep kit	Plasmid	178	178	pSB1C3-H6-0ZSortA	EB	50.0	276	50.0	383			A178	BI1930, BI1937, AG3768	ok, no result, ok
915	O178-3	O178		09-11	18:30	Silvan	Growth	676C3	Qiagen miniprep kit	Plasmid	178	178	pSB1C3-H6-0ZSortA	EB	50.0	288	50.0	384			A178	BI1931, BI1938, AG3769	H9R mutation, no result, ok
916	O177	O177		09-11	18:30	Silvan	Growth	675C1-1	Qiagen miniprep kit	Plasmid	177	177	pSB1C3-2ZSortA-H6	EB	50.0	249	50.0	385			A177	BI1926, BI1933	,
917	O177-2	O177		09-11	18:30	Silvan	Growth	675C2-1	Qiagen miniprep kit	Plasmid	177	177	pSB1C3-2ZSortA-H6	EB	50.0	256	50.0	386			A177	BI1927, BI1934	,
918	O177-3	O177		09-11	18:30	Silvan	Growth	675C3-1	Qiagen miniprep kit	Plasmid	177	177	pSB1C3-2ZSortA-H6	EB	50.0	224	50.0	387			A177	BI1928, BI1935	,
919	O188	O188		09-12	16:00	MaxW	Growth	705C1	Qiagen miniprep kit	Plasmid	188	188	pSBX1K3-LiLaLy(H6)	H2O	30.0	480	30	388	dont use, wrong stuff, discarded		G188	BI1939, BI1940	, luciferase??
920	O188-2	O188		09-12	16:00	MaxW	Growth	705C2	Qiagen miniprep kit	Plasmid	188	188	pSBX1K3-LiLaLy(H6)	H2O	30.0	149	30	389	dont use, probably wrong stuff, discarded		G188
921	O188-3	O188		09-12	16:00	MaxW	Growth	705C3	Qiagen miniprep kit	Plasmid	188	188	pSBX1K3-LiLaLy(H6)	H2O	30.0	463	30	390	dont use, wrong stuff, discarded		G188	BI1941, BI1942	, luciferase??
922	O189	O188		09-12	16:00	MaxW	Growth	706C1	Qiagen miniprep kit	Plasmid	188	188	pSBX1K3-LiLaLy(H6)	H2O	30.0	239	30	391	is pSBX1K3-LiLaLy(H6)		G189	BI1943, BI1944	, is pSBX1K3-LiLaLy(H6)
923	O189-2	O188		09-12	16:00	MaxW	Growth	706C2	Qiagen miniprep kit	Plasmid	188	188	pSBX1K3-LiLaLy(H6)	H2O	30.0	206	30	392	is pSBX1K3-LiLaLy(H6)		G189	BI1945, BI1946	, is pSBX1K3-LiLaLy(H6)
924	O189-3	O189		09-12	16:00	MaxW	Growth	706C3	Qiagen miniprep kit	Plasmid	189	189	pSBX4C5-2Zv2Npu:LaLys+flexlinker	H2O	30.0	256	30	393	dont use, wrong stuff,discarded		G189
925	O161	O161		09-12	17:30	Lisa	Growth	710C1	Qiagen miniprep kit	Plasmid	161	161	pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI	H2O	50.0	58	46.6	394			S161#5	BI1952	hä?
926	O161-2	O161		09-12	17:30	Lisa	Growth	710C2	Qiagen miniprep kit	Plasmid	161	161	pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI	H2O	50.0	27	42.7	395			S161#5
927	O161-3	O161		09-12	17:30	Lisa	Growth	710C3	Qiagen miniprep kit	Plasmid	161	161	pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI	H2O	50.0	56	46.5	396			S161#5
928	O182	O182		09-12	18:30	Caro	Growth	694C1	Qiagen miniprep kit	Plasmid	182	182	pSBX4C5-mRFPn1-154_mRFPc155-255_coex	H2O	30.0	681	30	397			S182	BI1948, BI1949	ok, ok
929	O182-2	O182		09-12	18:30	Caro	Growth	694C2	Qiagen miniprep kit	Plasmid	182	182	pSBX4C5-mRFPn1-154_mRFPc155-255_coex	H2O	30.0	586	30	398			S182	BI1950, BI1951	ok, ok
930	R265	R265		09-12	23:00	Caro	Gel	303-2,303-3	Gel extraction	Restriction	265		pSB1C3-BBa_E0240 (E/S)	H2O	30.0	10	20.1
931	R266	R266		09-12	23:00	Caro	Gel	303-4,303-5	Gel extraction	Restriction	266		pSB1C3-BBa_J06702 (E/S)	H2O	30.0	6	17.9
932	R267	R267		09-12	23:00	Caro	Gel	303-6,303-7	Gel extraction	Restriction	267		pSB4C5-BBa_J04450 (E/S)	H2O	30.0	14	18.0
933	R276	R276		09-13	18:00	Lisa	Gel	304-2, 304-3	Gel extraction	Restriction	276		pSB1C3-BBa_K808000 (E/S)	H2O	30.0	12	-0.4
934	R268	R268		09-13	19:00	Lisa	Oligos	271,272	Annealing	Restriction	268		EX-BsaI-CC-O-IasB-S	H2O	100.0	19	99.2
935	R269	R269		09-13	19:00	Lisa	Oligos	273,274	Annealing	Restriction	269		SsrA-Ctag-BsaI-Cext_BsaI	H2O	100.0	19	100.0
936	R270	R270		09-13	19:00	Lisa	Oligos	275,276	Annealing	Restriction	270		MTS_bs_minD_C_term	H2O	100.0	32	100.0
937	R271	R271		09-13	19:00	Lisa	Oligos	277,278	Annealing	Restriction	271		myristoilation-Ntag	H2O	100.0	14	100.0
938	R272	R272		09-13	19:00	Lisa	Oligos	279,280	Annealing	Restriction	272		NLS-Ntag	H2O	100.0	15	100.0
939	R273	R273		09-13	19:00	Lisa	Oligos	283,284	Annealing	Restriction	273		pel_B-Ntag	H2O	100.0	29	100.0
940	R274	R274		09-13	19:00	Lisa	Oligos	285,286	Annealing	Restriction	274		PKI_NES-Ntag	H2O	100.0	17	100.0
941	R275	R275		09-13	19:00	Lisa	Oligos	269,270	Annealing	Restriction	275		EX-BsaI-A-NN-IasB-S	H2O	100.0	19	99.2
942	O182-3	O182		09-13	22:00	Caro	Growth	694C4	Qiagen miniprep kit	Plasmid	182	182	pSBX4C5-mRFPn1-154_mRFPc155-255_coex	H2O	30.0	585	30.0		discarded		S182	BI1972, BI1973	ok, ok
943	O182-4	O182		09-13	22:00	Caro	Growth	694C5	Qiagen miniprep kit	Plasmid	182	182	pSBX4C5-mRFPn1-154_mRFPc155-255_coex	H2O	30.0		30.0		discarded		S182
944	O183	O183		09-13	22:00	Caro	Growth	695C4	Qiagen miniprep kit	Plasmid	183	183	pSBX4C5-mRFPn1-168-mRFPc169-255_coex	H2O	30.0	365	30.0				S183	BI1964, BI1965	ok, ok
945	O183-2	O183		09-13	22:00	Caro	Growth	695C5	Qiagen miniprep kit	Plasmid	183	183	pSBX4C5-mRFPn1-168-mRFPc169-255_coex	H2O	30.0		30.0		discarded		S183
946	O186	O186		09-13	22:00	Caro	Growth	698C2	Qiagen miniprep kit	Plasmid	186	186	pSBX4C5mCherryN_mCherryC_coex	H2O	30.0	466	30.0				S186	BI1966, BI1967	ok, ok
947	O186-2	O186		09-13	22:00	Caro	Growth	698C3	Qiagen miniprep kit	Plasmid	186	186	pSBX4C5mCherryN_mCherryC_coex	H2O	30.0		30.0		discarded		S186
948	O186-3	O186		09-13	22:00	Caro	Growth	698C4	Qiagen miniprep kit	Plasmid	186	186	pSBX4C5mCherryN_mCherryC_coex	H2O	30.0		30.0		discarded		S186
949	O186-4	O186		09-13	22:00	Caro	Growth	698C5	Qiagen miniprep kit	Plasmid	186	186	pSBX4C5mCherryN_mCherryC_coex	H2O	30.0		30.0		discarded		S186
950	O187	O187		09-13	22:00	Caro	Growth	699C1	Qiagen miniprep kit	Plasmid	187	187	pSBX4C5-GFPn1-157_GFPc158-255_coex	H2O	30.0	457	30.0				S187	BI1968, BI1969	ok, ok
951	O187-2	O187		09-13	22:00	Caro	Growth	699C2	Qiagen miniprep kit	Plasmid	187	187	pSBX4C5-GFPn1-157_GFPc158-255_coex	H2O	30.0		30.0		discarded		S187
952	O187-3	O187		09-13	22:00	Caro	Growth	699C3	Qiagen miniprep kit	Plasmid	187	187	pSBX4C5-GFPn1-157_GFPc158-255_coex	H2O	30.0		30.0		discarded		S187
953	O187-4	O187		09-13	22:00	Caro	Growth	699C4	Qiagen miniprep kit	Plasmid	187	187	pSBX4C5-GFPn1-157_GFPc158-255_coex	H2O	30.0		30.0		discarded		S187
954	O117-5	O117		09-14	23:00	Jan	Purification	O117-3	Dilution	Plasmid	117	117	pSB1C3-BBa_K741002	H20	100.0	2	97.0
955	O206	O206		09-14	23:00	Caro	Growth	726C1	Qiagen miniprep kit	Plasmid	206	206	pSB4C5-GFP_contr	H2O	30.0	513	7.1				S206	BI1959, BI1960	ok, ok
956	O206-2	O206		09-14	23:00	Caro	Growth	726C2	Qiagen miniprep kit	Plasmid	206	206	pSB4C5-GFP_contr	H2O	30.0		30.0				S206
957	O206-3	O206		09-14	23:00	Caro	Growth	726C3	Qiagen miniprep kit	Plasmid	206	206	pSB4C5-GFP_contr	H2O	30.0		30.0				S206
958	O205	O205		09-14	23:00	Caro	Growth	727C1	Qiagen miniprep kit	Plasmid	205	205	pSB4C5-mCherry_contr	H2O	30.0	542	24.5				S205	BI1961, BI1962	ok, ok
959	O205-2	O205		09-14	23:00	Caro	Growth	727C2	Qiagen miniprep kit	Plasmid	205	205	pSB4C5-mCherry_contr	H2O	30.0		30.0				S205
960	O205-3	O205		09-14	23:00	Caro	Growth	727C3	Qiagen miniprep kit	Plasmid	205	205	pSB4C5-mCherry_contr	H2O	30.0		30.0				S205
961	P175-2	P175		09-15	2:08	Jan	Gel	312-4, 312-5	Gel extraction	PP	175		pSB1C3-BBa_K741002_MT_SspDnaBN	H2O	20.0	20	18.0
962	O207	O207		09-15	11:00	Lisa	Growth	734C1	Qiagen miniprep kit	Plasmid	207	207	pSB1C3-EX-BsaI-CC-O-IasB-S	H2O	50.0	97	50.0	407			S207
963	O207-2	O207		09-15	11:00	Lisa	Growth	734C3	Qiagen miniprep kit	Plasmid	208	208	pSB1C3-EX-BsaI-A-NN-IasB-S	H2O	50.0	185	50.0	408	might be from colony 735C3 instead		S207	BI1953, BI1954	is O208-2......oo, 3.G afterprefix to AC, is O208-2......oo, 3.G afterprefix to AC
964	O208	O208		09-15	11:00	Lisa	Growth	735C1	Qiagen miniprep kit	Plasmid	208	208	pSB1C3-EX-BsaI-A-NN-IasB-S	H2O	50.0	105	50.0	409			S208
965	O208-2	O208		09-15	11:00	Lisa	Growth	735C3	Qiagen miniprep kit	Plasmid	207	207	pSB1C3-EX-BsaI-CC-O-IasB-S	H2O	50.0	140	50.0	410	might be from colony 734C3 instead		S208	BI1955, BI1956	is O207-2....:(, 3.G afterprefix to AC, is O207-2....:(, 3.G afterprefix to AC
966	P261	P261		09-14	15:00	Maggie	Gel	314-2,314-3	Gel extraction	PP	261		DNMT1(731-1602)_BsaI=CAAC-CWE_BsaI=CGCA	H2O	25.0	40	25.0
967	P262	P262		09-14	15:00	Maggie	Gel	314-4,314-5	Gel extraction	PP	262		ccdB_BsaI=CGCA=BsmBI_BsmBI=ATCA-H5SGRGK-TGCT=BsaI	H2O	25.0	73	25.0
968	O181-6	O181		09-14	12:00	Consti	Purification	O181-3	Dilution	Plasmid	181	181	pSBX1K3-Xylanase	H2O	39.0	1	35.0
969	R260-2	R260		09-15	12:15	MaxW	Oligos	200,201	Annealing	Restriction	260		flex-linker	H2O	100.0	2	100.0
970	R277	R277		09-15	12:15	MaxW	Oligos	244,245	Annealing	Restriction	277		H6-insert(Xyla)	H2O	100.0	2	100.0
971	R278	R278		09-15	12:15	MaxW	Oligos	246,247	Annealing	Restriction	278		H6-insert+exteins(Xyla)	H2O	100.0	3	100.0
972	O179	O179		09-14	17:00	Anna	Growth	709C2	Qiagen miniprep kit	Plasmid	179	179	pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6	H2O	30.0	453	30.0				G179#2	BI1957, AF9231	,
973	O179-2	O179		09-14	17:00	Anna	Growth	709C4	Qiagen miniprep kit	Plasmid	179	179	pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6	H2O	30.0	283	30.0				G179#2	BI1958, AF9232	,
974	O180-2	O180		09-15	18:20	Anna	Growth	693C1-1	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	H2O	50.0		50.0		discarded		O180	BI1970, BI1971	deletion in 1st Stop codon, deletion in 1st Stop codon
975	P304	P304		09-15	19:30	Consti	Gel	317-2, 317-3	Gel extraction	PP	304		XylaLin_BsmBI_IBmsB	H2O	20.0	18	20.0
976	P148-2	P148		09-14	21:00	Anna	Gel	319-2, 319-3	Gel extraction	PP	148		mRFPexp_BsaI=CGCA=BsmBI_BsmBI=CTTC=BsaI	H2O	20.0	168	20.0		vielleicht 218
977	P218-2	P218		09-14	21:00	Anna	Gel	319-4,319-5	Gel extraction	PP	218		CGCA=BsaI-PlacI-mRFP-BsaI=CTTC	H2O	20.0	184	20.0		vielleicht 218
978	O192	O192		09-17	1:00	Caro	Growth	768C1	Qiagen miniprep kit	Plasmid	192	192	pSBX4C5-1GateNpuDnaE(N)*-H6			407	0.0	427			G192#2	BI1980	looks like pSBX4C5-1GateNpuDnaEC_SN->AG_H6
979	O193	O193		09-17	1:00	Caro	Growth	769C1	Qiagen miniprep kit	Plasmid	193	193	pSBX4C5-1GateSspDnaX-S11(C)-H6			354	-2.8	428			G193#2	BI1982	ok
980	O194	O194		09-17	1:00	Caro	Growth	770C1	Qiagen miniprep kit	Plasmid	194	194	pSBX4C5-1GateSspDnaX-S11C HN->AG_H6			420	0.0	429			G194#2	BI1984	ok
981	O195	O195		09-17	1:00	Caro	Growth	771C1	Qiagen miniprep kit	Plasmid	195	195	pSBX4C5-1GateSspDnaX-S11N_H6			370	-2.7	430			G195#2	BI1986	ok
982	O196	O196		09-17	1:00	Caro	Growth	772C1	Qiagen miniprep kit	Plasmid	196	196	pSBX4C5-1GateSspDnaX-S11N C->G_H6			391	0.0	431			G196#2	BI1988	ok
983	O197	O197		09-17	1:00	Caro	Growth	773C1	Qiagen miniprep kit	Plasmid	197	197	pSBX4C5-1GateSspGyrB-S11C_H6			408	-2.5	432			G197#2	BI1990	ok
984	O198	O198		09-17	1:00	Caro	Growth	774C1	Qiagen miniprep kit	Plasmid	198	198	pSBX4C5-1GateSspGyrB-S11C SN->AG_H6			363	0.0	433			G198#2	BI1992	ok
985	O199	O199		09-17	1:00	Caro	Growth	775C1	Qiagen miniprep kit	Plasmid	199	199	pSBX4C5-1GateSspGyrB-S11N_H6			330	-3.0	434			G199#2	BI1994	ok
986	O200	O200		09-17	1:00	Caro	Growth	776C1	Qiagen miniprep kit	Plasmid	200	200	pSBX4C5-1GateSspGyrB-S11N C->G_H6			312	0.0	435			G200#2	BI1996	ok
987	O201	O201		09-17	1:00	Caro	Growth	777C1	Qiagen miniprep kit	Plasmid	201	201	pSBX4C5-1GateTerDnaE3-S11C_H6			432	-2.3	436			G201#2	BI1998	ok
988	O202	O202		09-17	1:00	Caro	Growth	778C1	Qiagen miniprep kit	Plasmid	202	202	pSBX4C5-1GateTerDnaE3-S11C SN->AG_H6			380	0.0	437			G202#2	BI2000	ok
989	O203	O203		09-17	1:00	Caro	Growth	779C1	Qiagen miniprep kit	Plasmid	203	203	pSBX4C5-1GateTerDnaE3-S11(N)-H6			459	-2.2	438			G203#2	BI2002	ok
990	O204	O204		09-17	1:00	Caro	Growth	780C1	Qiagen miniprep kit	Plasmid	204	204	pSBX4C5-1GateTerDnaE3-S11(N)*-H6			400	0.0	439			G204#2	BI2004	ok
991	P230-2	P230		09-17	1:15	Silvan	Gel	323-2,323-3	Gel extraction	PP	230		pSB1C3_mockSortAc_H6-Smt3	EB	15.0	33	10.0
992	P234-2	P234		09-17	1:15	Silvan	Gel	323-4,323-5	Gel extraction	PP	234		pSB1C3_SortAc-H6_TEVc-BsaI	EB	15.0	7	10.0
993	P232-2	P232		09-17	1:15	Silvan	Gel	323-6,323-7	Gel extraction	PP	232		pSB1C3_mockSortAc_H6-TEVc	EB	15.0	2	10.0
994	P238-2	P238		09-17	1:15	Silvan	Gel	323-12,323-13	Gel extraction	PP	238		H6Smt3_BBprefix_TEVc-BsaI	EB	15.0	82	15.0
995	P231-2	P231		09-17	1:15	Silvan	Gel	324-2,324-3	Gel extraction	PP	231		mRFPexp_Smt3-TEVc-BsaI_mockSortAc-BBsuffix	EB	15.0	78	15.0
996	P233-2	P233		09-17	1:15	Silvan	Gel	324-4,324-5	Gel extraction	PP	233		mRFPexp_H6-TEVc_mockSortAc-BBsuffix	EB	15.0	74	15.0
997	P235-2	P235		09-17	1:15	Silvan	Gel	324-6,324-7	Gel extraction	PP	235		mRFPexp_TEVc-BsaI_SortAc-H6-BBsuffix	EB	15.0	47	15.0
998	P230-3	P230		09-17	3:50	Silvan	Gel	328-2,328-3	Gel extraction	PP	230		pSB1C3_mockSortAc_H6-Smt3	EB	15.0	65	15.0
999	P234-3	P234		09-17	3:50	Silvan	Gel	328-4,328-5	Gel extraction	PP	234		pSB1C3_SortAc-H6_TEVc-BsaI	EB	15.0	78	15.0
1000	P232-3	P232		09-17	3:50	Silvan	Gel	328-6,328-7	Gel extraction	PP	232		pSB1C3_mockSortAc_H6-TEVc	EB	15.0	74	15.0
1001	P147-5	P147		09-17	4:20	Silvan	Gel	329-1,329-3	Gel extraction	PP	147		mDNMT1(731-1602)_BsaI=GGGT-GGG_CGCA=BsaI	EB	20.0	38	20.0
1002	O219	O219		09-17	15:26	Jan	Extern	Dominik Niopek		Plasmid	219	219	pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)	H20	500.0	1	487.0
1003	O162-3	O162		09-17	16:00	Anna	Growth	665C5	Qiagen miniprep kit	Plasmid	162	162	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)	H2O	50.0	310	50.0				S162#3
1004	O189-4	O189		09-17	16:00	MaxW	Growth	794C1	Qiagen miniprep kit	Plasmid	189	189	pSBX4C5-2Zv2Npu:LaLys+flexlinker	H2O	30.0	205	30.0	418	discarded		G189#2	BI1974, BI1975	ok, bad seq
1005	O189-5	O189		09-17	16:00	MaxW	Growth	794C2	Qiagen miniprep kit	Plasmid	189	189	pSBX4C5-2Zv2Npu:LaLys+flexlinker	H2O	30.0	224	30.0	419			G189#2	BI1976, BI1977	ok, ok
1006	O189-6	O189		09-17	16:00	MaxW	Growth	794C3	Qiagen miniprep kit	Plasmid	189	189	pSBX4C5-2Zv2Npu:LaLys+flexlinker	H2O	30.0	284	30.0	420	discarded		G189#2
1007	O216	O216		09-17	16:00	MaxW	Growth	795C1	Qiagen miniprep kit	Plasmid	216	216	pSBX1K3-XylaH6BB	H2O	50.0	177	50.0	421			G216
1008	O216-2	O216		09-17	16:00	MaxW	Growth	795C2	Qiagen miniprep kit	Plasmid	216	216	pSBX1K3-XylaH6BB	H2O	50.0	144	46.5	422			G216	BI1978	ok
1009	O216-3	O216		09-17	16:00	MaxW	Growth	795C3	Qiagen miniprep kit	Plasmid	216	216	pSBX1K3-XylaH6BB	H2O	50.0	135	50.0	423			G216
1010	O217	O217		09-17	16:00	MaxW	Growth	796C1	Qiagen miniprep kit	Plasmid	217	217	pSBX1K3-XylaH6extBB	H2O	50.0	171	50.0	424			G217	BI1979, BF8279	bad seq, bad seq
1011	O217-2	O217		09-17	16:00	MaxW	Growth	796C2	Qiagen miniprep kit	Plasmid	217	217	pSBX1K3-XylaH6extBB	H2O	50.0	130	46.1	425			G217	BF8280	ok
1012	O217-3	O217		09-17	16:00	MaxW	Growth	796C3	Qiagen miniprep kit	Plasmid	217	217	pSBX1K3-XylaH6extBB	H2O	50.0	145	50.0	426			G217
1013	O191	O191		09-17	18:00	Caro	Growth	811C1	Qiagen miniprep kit	Plasmid	191	191	pSBX4C5-1GateNpuDnaE(C)*-H6	H2O	30.0	314	30.0	440	discarded		G191#2	BI2006	looks like pSBX4C5-1GateNpuDnaEN_C->G_H6
1014	O191-2	O191		09-17	18:00	Caro	Growth	811C2	Qiagen miniprep kit	Plasmid	191	191	pSBX4C5-1GateNpuDnaE(C)*-H6	H2O	30.0	308	30.0	452	discarded		G191#2	BI2008	looks like pSBX4C5-1GateNpuDnaEN_C->G_H6
1015	O180-3	O180		09-18	18:00	Jakob	Growth	688C2-1	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	EB	50.0	69	50.0		discarded		P263	Bl2010	mutation in first stop codon as well, mutation in chromophore region, no C but S
1016	O180-4	O180		09-18	18:00	Jakob	Growth	688C3-1	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	EB	50.0	171	50.0		discarded		P263	Bl2011
1017	P323	P323		09-18	15:00	Lisa	PCR	1502	PCR purification kit	PP	323		SSpDnaBC_ccdB-CN_CC-mRFP	H2O	57.9	59	57.9
1018	P324	P324		09-18	15:00	Lisa	PCR	1503	PCR purification kit	PP	324		SSpDnaBC_ccdB-CN_CC*-mRFP	H2O	41.6	40	41.6
1019	P71-3	P71		09-18	todo	Lisa	PCR	1504	PCR purification kit	PP	71		pSBX4C5_ccdB-BsmBI_BsaI-mRFP_N				0.0	no PCR product
1020	P72-3	P72		09-18	todo	Lisa	PCR	1505	PCR purification kit	PP	72		pSBX4C5_mRFP-BsaI_BsmBI-ccdB_C				0.0
1021	R59	R59			todo	Lisa	PCR	273	Annealing	Restriction	59		pSB1T3 (E/P)	H2O	50.0	20	50.0		9/18/2014
1022	R60	R60			todo	Lisa	PCR	274	Annealing	Restriction	60		pSB1C3-BBa_E0240 (E/P)	H2O	50.0	20	50.0		9/18/2014
1023	O209	O209		09-18	18:45	Maggie	Growth	819C1	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)			88	-1.0	464			G209
1024	O209-2	O209		09-18	18:45	Maggie	Growth	819C2	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	465			G209
1025	O209-3	O209		09-18	18:45	Maggie	Growth	819C3	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	466			G209
1026	O209-4	O209		09-18	18:45	Maggie	Growth	819C4	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)			122	-1.0	467			G209	BI2012, IH6892	ok, ccdB missing
1027	O209-5	O209		09-18	18:45	Maggie	Growth	819C5	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	468			G209
1028	O209-6	O209		09-18	18:45	Maggie	Growth	819C6	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	469			G209	BI2013, IH6893	really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector), really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector)
1029	O209-7	O209		09-18	18:45	Maggie	Growth	819C7	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	470			G209
1030	O209-8	O209		09-18	18:45	Maggie	Growth	819C8	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)			50	-1.0	471			G209	BI2014, IH6894	no result (concentration probably was too low), no result (concentration probably was too low)
1031	O176	O176		09-18	18:45	Maggie	Growth	818C2	Qiagen miniprep kit	Plasmid	176	176	pSB1C3-H6-Smt3-0ZSortA			69	0.0	473			A176#2	AG3764, IH6895	ok, ok
1032	O176-2	O176		09-18	18:45	Maggie	Growth	818C3	Qiagen miniprep kit	Plasmid	176	176	pSB1C3-H6-Smt3-0ZSortA				0.0	474			A176#2	AG3765, IH6896	H6R mutation, ok
1033	O176-3	O176		09-18	18:45	Maggie	Growth	818C7	Qiagen miniprep kit	Plasmid	176	176	pSB1C3-H6-Smt3-0ZSortA			88	-17.1	477			A176#2	AG3766, IH6897	ok, ok
1034	P322	P322		09-19	2:53	Jan	PCR	1499	PCR purification kit	PP	322	220	pL157_C141T (=O220)	H2O	20.0		20.0
1035	P322-2	P322		09-19	2:53	Jan	PCR	1500	PCR purification kit	PP	322	220	pL157_C141T (=O220)	H2O	20.0		20.0
1036	O65-12	O65			never	MaxW	Growth	835C1	Qiagen maxiprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	EB	50.0		50.0		zuerst mit 50 eluieren		O65-8
1037	O65-13	O65			never	MaxW	Growth	835C1	Qiagen maxiprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	EB	350.0		350.0		dann nochmal mit 350		O65-8
1038	O210	O210		09-19	13:00	Lisa	Growth	820C1	Qiagen miniprep kit	Plasmid	210	210	pSB1C3-BsaI-CC-O-IasB-Ssra				0.0	479			G210#2
1039	O210-2	O210		09-19	13:00	Lisa	Growth	820C2	Qiagen miniprep kit	Plasmid	210	210	pSB1C3-BsaI-CC-O-IasB-Ssra				0.0	480			G210#2	Bl1963
1040	O210-3	O210		09-19	13:00	Lisa	Growth	820C3	Qiagen miniprep kit	Plasmid	210	210	pSB1C3-BsaI-CC-O-IasB-Ssra				0.0	481			G210#2
1041	O211	O211		09-19	13:00	Lisa	Growth	821C1	Qiagen miniprep kit	Plasmid	211	211	pSB1C3-BsaI-CC-O-IasB-MTS				0.0	482			G211#2
1042	O211-2	O211		09-19	13:00	Lisa	Growth	821C2	Qiagen miniprep kit	Plasmid	211	211	pSB1C3-BsaI-CC-O-IasB-MTS				0.0	483			G211#2	AG3770
1043	O211-3	O211		09-19	13:00	Lisa	Growth	821C3	Qiagen miniprep kit	Plasmid	211	211	pSB1C3-BsaI-CC-O-IasB-MTS				0.0	484			G211#2
1044	O212	O212		09-19	13:00	Lisa	Growth	822C1	Qiagen miniprep kit	Plasmid	212	212	pSB1C3-EX-BsaI-A-NN-IasB-myristoilation				0.0	485			G212#2
1045	O212-2	O212		09-19	13:00	Lisa	Growth	822C2	Qiagen miniprep kit	Plasmid	212	212	pSB1C3-EX-BsaI-A-NN-IasB-myristoilation				0.0	486			G212#2	AG3771
1046	O212-3	O212		09-19	13:00	Lisa	Growth	822C3	Qiagen miniprep kit	Plasmid	212	212	pSB1C3-EX-BsaI-A-NN-IasB-myristoilation				0.0	487			G212#2
1047	O213	O213		09-19	13:00	Lisa	Growth	823C1	Qiagen miniprep kit	Plasmid	213	213	pSB1C3-EX-BsaI-A-NN-IasB-NLS				0.0	488			G213#2
1048	O213-2	O213		09-19	13:00	Lisa	Growth	823C2	Qiagen miniprep kit	Plasmid	213	213	pSB1C3-EX-BsaI-A-NN-IasB-NLS				0.0	489			G213#2	AG3772
1049	O213-3	O213		09-19	13:00	Lisa	Growth	823C3	Qiagen miniprep kit	Plasmid	213	213	pSB1C3-EX-BsaI-A-NN-IasB-NLS				0.0	490			G213#2
1050	O214	O214		09-19	13:00	Lisa	Growth	824C1	Qiagen miniprep kit	Plasmid	214	214	pSB1C3-EX-BsaI-A-NN-IasB-pelB				0.0	491			G214#2
1051	O214-2	O214		09-19	13:00	Lisa	Growth	824C2	Qiagen miniprep kit	Plasmid	214	214	pSB1C3-EX-BsaI-A-NN-IasB-pelB				0.0	492			G214#2	AG3773
1052	O214-3	O214		09-19	13:00	Lisa	Growth	824C3	Qiagen miniprep kit	Plasmid	214	214	pSB1C3-EX-BsaI-A-NN-IasB-pelB				0.0	493			G214#2
1053	O215	O215		09-19	13:00	Lisa	Growth	825C1	Qiagen miniprep kit	Plasmid	215	215	pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES				0.0	494			G215#2
1054	O215-2	O215		09-19	13:00	Lisa	Growth	825C2	Qiagen miniprep kit	Plasmid	215	215	pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES				0.0	495			G215#2
1055	O215-3	O215		09-19	13:00	Lisa	Growth	825C3	Qiagen miniprep kit	Plasmid	215	215	pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES				0.0	496			G215#2
1056	O218	O218		09-19	13:00	Lisa	Growth	826C1	Qiagen miniprep kit	Plasmid	218	218	pSB1A2-BBa_K103006				0.0	497			D2012-2-22P
1057	O218-2	O218		09-19	13:00	Lisa	Growth	826C2	Qiagen miniprep kit	Plasmid	218	218	pSB1A2-BBa_K103006	H2O	50.0	90	50.0	498			D2012-2-22P
1058	O218-3	O218		09-19	13:00	Lisa	Growth	826C3	Qiagen miniprep kit	Plasmid	218	218	pSB1A2-BBa_K103006				0.0	499			D2012-2-22P
1059	O209-9	O209		09-18	18:00	Maggie	Growth	819C1	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	464			G209
1060	O209-10	O209		09-18	18:00	Maggie	Growth	819C2	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	465			G209
1061	O209-11	O209		09-18	18:00	Maggie	Growth	819C3	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	466			G209
1062	O209-12	O209		09-18	18:00	Maggie	Growth	819C4	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	467			G209
1063	O209-13	O209		09-18	18:00	Maggie	Growth	819C5	Qiagen miniprep kit	Plasmid	209	209	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)				0.0	468			G209
1064	R284	R284		09-19	18:00	Jakob	Gel	343-2-1, 343-3-1	Gel extraction	Restriction	284		pSBX4C5-BBa_I13507 (E/S)		30.0	16	16.9
1065	R285	R285		09-19	18:00	Jakob	Gel	343-4-2, 343-5-2	Gel extraction	Restriction	285		pSB4C5-mCherry_contr (E/S)		30.0	9	20.1
1066	R286	R286		09-19	18:00	Jakob	Gel	343-6-2, 343-7-2	Gel extraction	Restriction	286		pSB4C5-GFP_contr (E/S)		30.0	7	19.1
1067	O175	O175		09-19	18:00	Anna	Growth	829C2	Qiagen miniprep kit	Plasmid	175	175	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			194	0.0				G175#6	BI1985, BI2003	ok, ok
1068	O175-2	O175		09-19	18:00	Anna	Growth	829C3	Qiagen miniprep kit	Plasmid	175	175	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			141	0.0				G175#6	BI1987, BI2005	ok, ok
1069	O175-3	O175		09-19	18:00	Anna	Growth	829C5	Qiagen miniprep kit	Plasmid	175	175	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			209	0.0				G175#6	BI1989, BI2007	ok, ok
1070	O175-4	O175		09-19	18:00	Anna	Growth	831C1	Qiagen miniprep kit	Plasmid	175	175	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			102	0.0		190		G175#7	BI1991, BI2009	really is BsmBI version; ok, really is BsmBI version; ok
1071	O175-5	O175		09-19	18:00	Anna	Growth	832C2	Qiagen miniprep kit	Plasmid	175	175	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			247	0.0				G175#6	BI1993, AF9233	ok, ok
1072	O175-6	O175		09-19	18:00	Anna	Growth	834C2	Qiagen miniprep kit	Plasmid	175	175	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			341	0.0		190		G175#7	BI1995, AF9234	really is BsmBI version; ok, really is BsmBI version; ok
1073	O175-7	O175		09-19	18:00	Anna	Growth	834C3	Qiagen miniprep kit	Plasmid	175	175	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			88	0.0		190		G175#7	BI1997, AF9235	really is BsmBI version; ok, really is BsmBI version; ok
1074	O190	O190		09-19	18:00	Anna	Growth	834C5	Qiagen miniprep kit	Plasmid	190	190	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6			277	0.0				G175#7	BI1999, AF9236	ok, ok
1075	O190-2	O190		09-19	18:00	Anna	Growth	833C4	Qiagen miniprep kit	Plasmid	190	190	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6			75	0.0				G190#6	BI2001, II2292	ok, ok
1076	R287	R287		09-21	11:30	Jakob	Gel	350-2-1, 350-3-1	Gel extraction	Restriction	287		pSBX4C5-BBa_I13507 (E/P)	H2O	30.0	27	16.6
1077	R288	R288		09-21	11:30	Jakob	Gel	350-4-2, 350-5-2	Gel extraction	Restriction	288		pSB4C5-mCherry_contr (E/P)	H2O	30.0	17	20.4
1078	R289	R289		09-21	11:30	Jakob	Gel	350-6-2, 350-7-2	Gel extraction	Restriction	289		pSB4C5-GFP_contr (E/P)	H2O	30.0	13	19.1
1079	O62-11	O62		09-21	11:30	Jakob	Growth	327C6-1	Qiagen midiprep kit	Plasmid	62	62	pSBX4C5-BBa_I13507	H2O	200.0	320	193.7		not sure if I accidentally swaped tubes. we will see iafter digest		O62
1080	O104-3	O104		09-21	11:30	Jakob	Growth	434C1-7	Qiagen midiprep kit	Plasmid	104	104	pSB1C3-BBa_I746908	H2O	200.0	238	191.6		not sure if I accidentally swaped tubes. we will see iafter digest		D2014-4-5C
1081	O223	O223		09-21	12:30	Jakob	Growth	860C1	Qiagen miniprep kit	Plasmid	223	223	pSBX4C5-0GateSspDnaX-S11C_mRFP169-225	H2O	30.0	542	28.2				G223	BF8267	ok good
1082	O224	O224		09-21	12:30	Jakob	Growth	861C1	Qiagen miniprep kit	Plasmid	224	224	pSBX4C5-0GateSspDnaX-S11N_mRFP1-168	H2O	30.0	472	25.8				G224	BF8268	ok good
1083	O225	O225		09-21	12:30	Jakob	Growth	862C1	Qiagen miniprep kit	Plasmid	225	225	pSBX4C5-0GateSspGyrB-S11C_mRFP169-225	H2O	30.0	571	28.2				G225	BF8270	voller mutationen -> falsches FP insert? NEU, use O225-2
1084	O226	O226		09-21	12:30	Jakob	Growth	863C1	Qiagen miniprep kit	Plasmid	226	226	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168	H2O	30.0	304	26.7		no purification since red color, but growths on old plates with BL21		G226	BF8271	frameshift mutation? NEU,useO226-4
1085	O227	O227		09-21	12:30	Jakob	Growth	864C1	Qiagen miniprep kit	Plasmid	227	227	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225	H2O	30.0	544	28.2				G227	BF8272	kein insert? NEU
1086	O228	O228		09-21	12:30	Jakob	Growth	865C1	Qiagen miniprep kit	Plasmid	228	228	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168	H2O	30.0	878	28.9				G228	BF8273	mutation im prefix? but less mutation than O228-2,use O228-4
1087	O229	O229		09-21	12:30	Jakob	Growth	866C1	Qiagen miniprep kit	Plasmid	229	229	pSBX4C5-0GateNpuDnaE_C_mRFP169-225	H2O	30.0	524	28.1		one colony on BL21 plate		G229	BF8275	OK good
1088	O230	O230		09-21	12:30	Jakob	Growth	867C1	Qiagen miniprep kit	Plasmid	230	230	pSBX4C5-0GateNpuDnaE_N_mRFP1-168	H2O	30.0	300	26.7		no purification since red color, but growths on old plates with BL21		G230	BF8276	mutation in mRFP G toD
1089	O220	O220		09-21	12:30	Jakob	Growth	843C1	Qiagen miniprep kit	Plasmid	220	220	pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T	H2O	30.0	452	30.0	508			P322	BF8261, BF8262, BF8281, BF8282	, , good, but wrong -> no mutation, good, but wrong -> no mutation
1090	O220-2	O220		09-21	12:30	Jakob	Growth	844C1	Qiagen miniprep kit	Plasmid	220	220	pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T	H2O	30.0	420	30.0	509			P322-2	BF8263, BF8264, BF8283, BF8284	, , ok, good!, ok, good!
1091	O220-3	O220		09-21	12:30	Jakob	Growth	844C2	Qiagen miniprep kit	Plasmid	220	220	pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T	H2O	30.0	421	30.0	510			P322-2	BF8265, BF8266, BF8285, BF8286	, , ok, good!, ok, good!
1092	O228-2	O228		09-21	12:30	Jakob	Growth	853C1	Qiagen miniprep kit	Plasmid	228	228	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168	H2O	30.0	276	26.4	505			G228	BF8274	mutation im prefix?
1093	O192-2	O192		09-21	12:30	Jakob	Growth	856C1	Qiagen miniprep kit	Plasmid	192	192	pSBX4C5-1GateNpuDnaE(N)*-H6	H2O	30.0	392	27.5				G192#3	BF8278	ok short sequencing
1094	O191-3	O191		09-21	12:30	Jakob	Growth	857C2	Qiagen miniprep kit	Plasmid	191	191	pSBX4C5-1GateNpuDnaE(C)*-H6	H2O	30.0	441	27.7	507	ok, lost		G191#3	BF8277	ok short sequencing
1095	O180-5	O180		09-21	0:30	Jakob	Growth	688C4	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	H2O	30.0	119	30.0				P263	BF8258	no mutation has been introduced and frame shift in stop codon
1096	O180-6	O180		09-21	0:30	Jakob	Growth	688C5	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	H2O	30.0	140	30.0		discarded		P263	BF8259	ok good
1097	O180-7	O180		09-21	0:30	Jakob	Growth	688C6	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	H2O	30.0	147	30.0				P263	BF8260	no mutation has been introduced and frame shift in stop codon
1098	P72-4	P72		09-22	16:00	Lisa	PCR	1561	PCR purification kit	PP	72		pSBX4C5_mRFP-BsaI_BsmBI-ccdB_C	H2O	30.0	57	30.0
1099	P71-4	P71		09-22	16:00	Lisa	PCR	1590	PCR purification kit	PP	71		pSBX4C5_ccdB-BsmBI_BsaI-mRFP_N	H2O	30.0	105	30.0
1100	O226-2	O226		09-21	8:00	Caro	Growth	851C1	Qiagen miniprep kit	Plasmid	226	226	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168			110	0.0				G226
1101	O230-2	O230		09-21	8:00	Caro	Growth	855C1	Qiagen miniprep kit	Plasmid	230	230	pSBX4C5-0GateNpuDnaE_N_mRFP1-168			121	0.0				G230
1102	O224-2	O224		09-21	8:00	Caro	Growth	861C2	Qiagen miniprep kit	Plasmid	224	224	pSBX4C5-0GateSspDnaX-S11N_mRFP1-168			123	-8.1				G224	BF8269	ok good
1103	O228-3	O228		09-21	8:00	Caro	Growth	865C2	Qiagen miniprep kit	Plasmid	228	228	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168			276	0.0				G228
1104	R304	R304		09-22	16:15	MaxW	Oligos	369,370	Annealing	Restriction	304		bad1#1	H2O	500.0	4	500.0
1105	R305	R305		09-22	16:15	MaxW	Oligos	371,372	Annealing	Restriction	305		bad1#2	H2O	500.0	4	500.0
1106	R306	R306		09-22	16:15	MaxW	Oligos	373,374	Annealing	Restriction	306		may1#1	H2O	500.0	5	500.0
1107	R307	R307		09-22	16:15	MaxW	Oligos	375,376	Annealing	Restriction	307		may1#2	H2O	500.0	5	500.0
1108	R308	R308		09-22	16:15	MaxW	Oligos	377,378	Annealing	Restriction	308		sgt1	H2O	500.0	3	500.0
1109	R309	R309		09-22	16:15	MaxW	Oligos	379,380	Annealing	Restriction	309		sgt2	H2O	500.0	5	500.0
1110	R310	R310		09-22	16:15	MaxW	Oligos	381,382	Annealing	Restriction	310		sho1	H2O	500.0	1	500.0
1111	R311	R311		09-22	16:15	MaxW	Oligos	383,384	Annealing	Restriction	311		sho2	H2O	500.0	2	500.0
1112	O218-4	O218		09-22	16:30	Lisa	Purification	O218-2	Dilution	Plasmid	218	218	pSB1A2-BBa_K103006	H2O	100.0	1	98.0
1113	R298	R298		09-22	19:00	Caro	Gel	354-2, 354-3	Gel extraction	Restriction	298		pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 (E/S)			15	-6.2
1114	R331	R331		09-22	19:00	Caro	Gel	354-4,354-5	Gel extraction	Restriction	331		pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 (E/S)			4	-18.5
1115	R299	R299		09-22	19:00	Caro	Gel	354-6,354-7	Gel extraction	Restriction	299		pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 (E/S)			10	-10.7
1116	R332	R332		09-22	19:00	Caro	Gel	354-8,354-9	Gel extraction	Restriction	332		pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)			5	-17.3
1117	R300	R300		09-22	19:00	Caro	Gel	354-11, 354-12	Gel extraction	Restriction	300		pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)			13	-7.6
1118	R303	R303		09-22	19:00	Caro	Gel	355-2,355-3	Gel extraction	Restriction	303		pSBX4C5-1GateNpuDnaE(N)*-H6 (E/S)			8	-15.2
1119	R294	R294		09-22	19:00	Caro	Restriction	294	PCR purification kit	Restriction	294		pSBX4C5-0GateSspDnaX-S11C_mRFP169-225 (E/X)			12	-15.9
1120	R295	R295		09-22	19:00	Caro	Restriction	295	PCR purification kit	Restriction	295		pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 (E/X)			7	-14.8
1121	R296	R296		09-22	19:00	Caro	Restriction	296	PCR purification kit	Restriction	296		pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 (E/X)			13	-16.6
1122	R297	R297		09-22	19:00	Caro	Restriction	297	PCR purification kit	Restriction	297		pSBX4C5-0GateNpuDnaE_C_mRFP169-225 (E/X)			5	-17.6
1123	R302	R302		09-22	19:00	Caro	Restriction	302	PCR purification kit	Restriction	302		pSBX4C5-1GateNpuDnaE(C)*-H6 (E/X)			11	-10.3
1124	P261-2	P261		09-22	20:20	Silvan	Gel	356-2-2.6kb,356-3-2.6kb	Gel extraction	PP	261		DNMT1(731-1602)_BsaI=CAAC-CWE_BsaI=CGCA	EB	30.0	72	30.0
1125	P332	P332		09-22	20:20	Silvan	Gel	356-4-2.6kb,356-5-2.6kb	Gel extraction	PP	332		DNMT1_BsaI=CAAC-CWE_H6SGRGT-TGCT=BsaI	EB	30.0	43	30.0
1126	P333	P333		09-22	20:20	Silvan	Gel	356-6-2.6kb,356-7-2.6kb	Gel extraction	PP	333		mDNMT1(731-1602)_BsaI=GGGT-G_CGCA=BsaI	EB	30.0	65	30.0
1127	P218-3	P218		09-22	20:20	Silvan	Gel	356-8-1.1kb,356-9-1.1kb	Gel extraction	PP	218		CGCA=BsaI-PlacI-mRFP-BsaI=CTTC	EB	30.0	111	30.0
1128	P341	P341		09-22	20:20	Silvan	Gel	356-10-1.1kb,356-11-1.1kb	Gel extraction	PP	341		mRFP_BsmBIBsaI_BsmBI=ATCA-H5SGRGT-TGCT=BsaI	EB	30.0	109	30.0
1129	P262-2	P262		09-22	20:20	Silvan	Gel	356-12-750bp,356-13-750bp,	Gel extraction	PP	262		ccdB_BsaI=CGCA=BsmBI_BsmBI=ATCA-H5SGRGK-TGCT=BsaI	EB	30.0	98	30.0
1130	R312	R312		09-22	16:15	Max W	Oligos	392,393	Annealing	Restriction	312		DNMT1lnk_0917/1r_p1			4	0.0
1131	R313	R313		09-22	16:15	Max W	Oligos	394,395	Annealing	Restriction	313		DNMT1lnk_0917/1r_p2			4	0.0
1132	R314	R314		09-22	16:15	Max W	Oligos	396,397	Annealing	Restriction	314		DNMT1lnk_0917/1r_p3			4	0.0
1133	R315	R315		09-22	16:15	Max W	Oligos	398,399	Annealing	Restriction	315		DNMT1lnk_0917/2r_p1			4	0.0
1134	R316	R316		09-22	16:15	Max W	Oligos	400,401	Annealing	Restriction	316		DNMT1lnk_0917/2r_p2			3	0.0
1135	R317	R317		09-22	16:15	Max W	Oligos	402,403	Annealing	Restriction	317		DNMT1lnk_0917/2r_p3			3	0.0
1136	R318	R318		09-20	19:00	Silvan	Oligos	265,266	Annealing	Restriction	318		DNMT1lnk_0825/1r_p1			5	0.0
1137	R319	R319		09-20	19:00	Silvan	Oligos	267,268	Annealing	Restriction	319		DNMT1lnk_0825/1r_p2			6	0.0
1138	R320	R320		09-20	19:00	Silvan	Oligos	263,303	Annealing	Restriction	320		DNMT1lnk_0901/1f			7	0.0
1139	R321	R321		09-20	19:00	Silvan	Oligos	320,312	Annealing	Restriction	321		DNMT1lnk_0915/1r_p1			4	0.0
1140	R322	R322		09-20	19:00	Silvan	Oligos	322,323	Annealing	Restriction	322		DNMT1lnk_0915/1r_p2			4	0.0
1141	R323	R323		09-20	19:00	Silvan	Oligos	324,325	Annealing	Restriction	323		DNMT1lnk_0915/1r_p3			4	0.0
1142	R324	R324		09-20	19:00	Silvan	Oligos	312,313	Annealing	Restriction	324		DNMT1lnk_0915/2r_p1			5	0.0
1143	R325	R325		09-20	19:00	Silvan	Oligos	314,315	Annealing	Restriction	325		DNMT1lnk_0915/2r_p2			5	0.0
1144	R326	R326		09-20	19:00	Silvan	Oligos	305,306	Annealing	Restriction	326		DNMT1lnk_0915/3f_p1			4	0.0
1145	R327	R327		09-20	19:00	Silvan	Oligos	307,308	Annealing	Restriction	327		DNMT1lnk_0915/3f_p2			4	0.0
1146	R328	R328		09-20	19:00	Silvan	Oligos	304,311	Annealing	Restriction	328		DNMT1lnk_0915/4f_p2			6	0.0
1147	R329	R329		09-20	19:00	Silvan	Oligos	316,317	Annealing	Restriction	329		DNMT1lnk_0915/6f_p1			4	0.0
1148	R330	R330		09-20	19:00	Silvan	Oligos	318,319	Annealing	Restriction	330		DNMT1lnk_0915/6f_p2			4	0.0
1149	R301	R301		09-22	20:00	Caro	Restriction	301	PCR purification kit	Restriction	301		pSBX4C5-0GateNpuDnaE_N_mRFP1-168 (E/S)			8	-7.9
1150	O221	O221		09-23	11:00:00	Jakob	Growth	874C1	Qiagen miniprep kit	Plasmid	221	221	pSBX4C5-mCherry_contr	H2O	30.0	703	30.0				S221#2
1151	O222	O222		09-23	11:00:00	Jakob	Growth	876C1	Qiagen miniprep kit	Plasmid	222	222	pSBX4C5-GFP_contr	H2O	30.0	947	30.0				S222#2
1152	P338	P338		09-23	10:00	Lisa	Oligos	293,295	Mix	PP	338		SspDnaBN_F_mRFP-NN	H2O	100.0	36	100.0
1153	P339	P339		09-23	10:00	Lisa	Oligos	294,295	Mix	PP	339		SspDnaBN_F_mRFP-NN*	H2O	100.0	40	100.0
1154	P340	P340		09-23	11:30	Lisa	PCR	1591	PCR purification kit	PP	340		OmpA_linker-BsaI_	H2O	30.0	67	30.0
1155	O107-3	O107		09-23	14:50	Jakob	Purification	O107	Dilution	Plasmid	107	107	pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	H2O	270.00	1	258.00
1156	O59-13	O59		09-23	14:50	Jakob	Purification	O59-12	Dilution	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O	822.80	1	818.80
1157	O225-2	O225		09-23	18:00	Caro	Growth	862C2	Qiagen miniprep kit	Plasmid	225	225	pSBX4C5-0GateSspGyrB-S11C_mRFP169-225	H2O	30.0	690	28.6				G225	BF8287	ok,good
1158	O225-3	O225		09-23	18:00	Caro	Growth	862C3	Qiagen miniprep kit	Plasmid	225	225	pSBX4C5-0GateSspGyrB-S11C_mRFP169-225	H2O	30.0		30.0				G225	BF8288	no mRFPc insert
1159	O226-3	O226		09-23	18:00	Caro	Growth	863C2	Qiagen miniprep kit	Plasmid	226	226	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168	H2O	30.0		30.0				G226	BF8289	frame shift in beginning
1160	O226-4	O226		09-23	18:00	Caro	Growth	863C3	Qiagen miniprep kit	Plasmid	226	226	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168	H2O	30.0	440	27.7				G226	BF8290	ok, good
1161	O227-2	O227		09-23	18:00	Caro	Growth	864C2	Qiagen miniprep kit	Plasmid	227	227	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225	H2O	30.0		30.0				G227	BF8291	no mRFPc insert
1162	O227-3	O227		09-23	18:00	Caro	Growth	864C3	Qiagen miniprep kit	Plasmid	227	227	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225	H2O	30.0		30.0				G227	BF8292	no mRFPc insert
1163	O229-2	O229		09-23	18:00	Caro	Growth	873C1	Qiagen miniprep kit	Plasmid	229	229	pSBX4C5-0GateNpuDnaE_C_mRFP169-225	H2O	30.0		30.0	512			O229	BF8294	ok, good
1164	O228-4	O228		09-23	18:00	Caro	Growth	872C1	Qiagen miniprep kit	Plasmid	228	228	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168	H2O	30.0	660	28.5	511			O228	BF8293	ok, but one mutation in LacOperon
1165	P342	P342		09-23	22:30	Jakob	Gel	360-2-1, 360-3-1, 360-4-1, 360-5-1	Gel extraction	PP	342		pSBX1K3-H6-H6 backbone	H2O	50.0	28	50.0
1166	P342-2	P342		09-23	22:30	Jakob	Gel	360-6-1, 360-7-1, 360-8-1, 360-9-1	Gel extraction	PP	342		pSBX1K3-H6-H6 backbone	H2O	50.0	31	50.0
1167	P344	P344		09-23	22:30	Jakob	Gel	360-12-1, 360-13-1	Gel extraction	PP	344	261	pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha (=261)	H2O	30.0		30.0
1168	O223-2	O223		09-23	23:00	Jakob	Purification	O223	Dilution	Plasmid	223	223	pSBX4C5-0GateSspDnaX-S11C_mRFP169-225	H2O	572.0	33	572.0		is O223 directly diluted
1169	O223-3	O223		09-23	11:00	Jakob	Purification	O223-2	Dilution	Plasmid	223	223	pSBX4C5-0GateSspDnaX-S11C_mRFP169-225	H2O	33.00	1	28.00
1170	O244	O244		09-24	13:00	MaxW	Growth	880C1	Qiagen miniprep kit	Plasmid	244	244	pSBX4C5-2Zv2Npu:LaLys+bad1	H2O	30.0	503	25.1	513			G244	28HD73	ok
1171	O244-2	O244		09-24	13:00	MaxW	Growth	880C2	Qiagen miniprep kit	Plasmid	244	244	pSBX4C5-2Zv2Npu:LaLys+bad1	H2O	30.0	556	30.0	514			G244
1172	O245	O245		09-24	13:00	MaxW	Growth	881C1	Qiagen miniprep kit	Plasmid	245	245	pSBX4C5-2Zv2Npu:LaLys+may1	H2O	30.0	384	28.8	515			G245	28HD74	ok
1173	O245-2	O245		09-24	13:00	MaxW	Growth	881C2	Qiagen miniprep kit	Plasmid	245	245	pSBX4C5-2Zv2Npu:LaLys+may1	H2O	30.0	481	30.0	516			G245
1174	O246	O246		09-24	13:00	MaxW	Growth	882C1	Qiagen miniprep kit	Plasmid	246	246	pSBX4C5-2Zv2Npu:LaLys+sgt1	H2O	30.0	465	29.0	517			G246	28HD75	kacke
1175	O246-2	O246		09-24	13:00	MaxW	Growth	882C2	Qiagen miniprep kit	Plasmid	246	246	pSBX4C5-2Zv2Npu:LaLys+sgt1	H2O	30.0	356	30.0	518			G246
1176	O247	O247		09-24	13:00	MaxW	Growth	883C1	Qiagen miniprep kit	Plasmid	247	247	pSBX4C5-2Zv2Npu:LaLys+sgt2	H2O	30.0	491	29.1	519			G247	28HD76	kacke
1177	O247-2	O247		09-24	13:00	MaxW	Growth	883C2	Qiagen miniprep kit	Plasmid	247	247	pSBX4C5-2Zv2Npu:LaLys+sgt2	H2O	30.0	204	30.0	520			G247
1178	O248	O248		09-24	13:00	MaxW	Growth	884C1	Qiagen miniprep kit	Plasmid	248	248	pSBX4C5-2Zv2Npu:LaLys+sho1	H2O	30.0	625	29.3	521			G248	28HD77	ok
1179	O248-2	O248		09-24	13:00	MaxW	Growth	884C2	Qiagen miniprep kit	Plasmid	248	248	pSBX4C5-2Zv2Npu:LaLys+sho1	H2O	30.0	426	30.0	522			G248
1180	O249	O249		09-24	13:00	MaxW	Growth	885C1	Qiagen miniprep kit	Plasmid	249	249	pSBX4C5-2Zv2Npu:LaLys+sho2	H2O	30.0	421	28.9	523			G249	28HD78	ok
1181	O249-2	O249		09-24	13:00	MaxW	Growth	885C2	Qiagen miniprep kit	Plasmid	249	249	pSBX4C5-2Zv2Npu:LaLys+sho2	H2O	30.0	639	30.0	524			G249
1182	P348	P348		09-24	3:20	Jakob	Gel	366-2-1, 366-3-1	Gel extraction	PP	348		mRFP169-225_H6-SspDnaXC_H6	H2O	30.0	105	30.0
1183	P349	P349		09-24	3:20	Jakob	Gel	366-4-1, 366-5-1	Gel extraction	PP	349		mRFP169-255_H6-SspDnaXC*_H6	H2O	30.0	94	30.0
1184	P350	P350		09-24	3:20	Jakob	Gel	366-6-1, 366-7-1	Gel extraction	PP	350		mRFP169-225_-6-SspDnaXC-Ext_H6	H2O	30.0	87	30.0
1185	P351	P351		09-24	3:20	Jakob	Gel	366-8-1, 366-9-1	Gel extraction	PP	351		mRFP169-225_-12-SspDnaXC-Ext-Jalpha_H6	H2O	30.0	82	30.0
1186	P352	P352		09-24	3:20	Jakob	Gel	366-10-1, 366-11-1	Gel extraction	PP	352		mRFP169-225_BsaIIasB_H6	H2O	30.0	70	30.0
1187	R333	R333		09-24	4:00	Silvan	Gel	368-1,368-2	Gel extraction	Restriction	333		pSBX1K3-T7RBS_BBv2 (S/P)	EB	20.0	33	17.7
1188	R334	R334		09-24	4:00	Silvan	Gel	368-3,368-4	Gel extraction	Restriction	334		pSB1C3-H6-Smt3-0ZSortA (X/P)	EB	20.0	20	16.2
1189	R335	R335		09-24	4:00	Silvan	Gel	368-6,368-7	Gel extraction	Restriction	335		pSB1C3-H6-0ZSortA (X/P)	EB	20.0	16	16.2
1190	R336	R336		09-24	4:00	Silvan	Gel	367-1,367-3	Gel extraction	Restriction	336		pSB1A3-Smt3-2ZSortA-H6 (ccdB) (E/P)	EB	20.0	19	16.7
1191	R337	R337		09-24	4:00	Silvan	Gel	368-8	Gel extraction	Restriction	337		pSB1C3-BBa_J04450 (E/P)	EB	20.0	11	16.3
1192	O108-5	O108		09-24	9:00		Purification	O108-4	Dilution	Plasmid	108	108	pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	H2O	500.0	1	498.0
1193	O105-3	O105		09-24	9:00		Purification	O105-2	Dilution	Plasmid	105	105	pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	H2O	222.0	1	216.0
1194	O220-4	O220		09-24	9:00		Purification	O220	Dilution	Plasmid	220	220	pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T	H2O	420.0	1	414.0
1195	O195-2	O195		09-24	9:00		Purification	O195	Dilution	Plasmid	195	195	pSBX4C5-1GateSspDnaX-S11N_H6	H2O	370.0	1	368.0
1196	O106-3	O106		09-24	9:00		Purification	O106	Dilution	Plasmid	106	106	pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6	H2O	210.0	1	208.0
1197	O232	O232		09-24	13:00	Maggie	Growth	907C1	Qiagen miniprep kit	Plasmid	232	232	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT)			145	0.0	525			G232	DD1384, DD1391	ok, ok
1198	O232-2	O232		09-24	13:00	Maggie	Growth	907C2	Qiagen miniprep kit	Plasmid	232	232	pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT)			107	0.0	526			G232	DD1385, DD1392	ok, frameshift mutation behind DNMT1
1199	O231	O231		09-24	13:00	Maggie	Growth	908C2	Qiagen miniprep kit	Plasmid	231	231	pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			79	0.0	527			G231	DD1386, DD1393	,
1200	O231-2	O231		09-24	13:00	Maggie	Growth	909C1	Qiagen miniprep kit	Plasmid	231	231	pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			102	0.0	528			G231
1201	O231-3	O231		09-24	13:00	Maggie	Growth	909C2	Qiagen miniprep kit	Plasmid	231	231	pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6			113	0.0	529			G231
1202	O239	O239		09-24	13:00	Maggie	Growth	910C1	Qiagen miniprep kit	Plasmid	239	239	pSBX1K3-T7RBS-2ZSortA:eGFP-H6			124	0.0	530			G239
1203	O239-2	O239		09-24	13:00	Maggie	Growth	910C2	Qiagen miniprep kit	Plasmid	239	239	pSBX1K3-T7RBS-2ZSortA:eGFP-H6			138	-7.5	531			G239	28HE02	ok
1204	O239-3	O239		09-24	13:00	Maggie	Growth	911C1	Qiagen miniprep kit	Plasmid	239	239	pSBX1K3-T7RBS-2ZSortA:eGFP-H6			44	-7.5	532			G239	DD1387, DD139428HE01	no result, no resultok
1205	O239-4	O239		09-24	13:00	Maggie	Growth	911C2	Qiagen miniprep kit	Plasmid	239	239	pSBX1K3-T7RBS-2ZSortA:eGFP-H6			41	0.0	533			G239
1206	O233	O233		09-24	13:00	Maggie	Growth	912C1	Qiagen miniprep kit	Plasmid	233	233	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)			58	0.0				G233
1207	O233-2	O233		09-24	13:00	Maggie	Growth	913C1	Qiagen miniprep kit	Plasmid	233	233	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)			41	0.0	535			G233
1208	O233-3	O233		09-24	13:00	Maggie	Growth	913C2	Qiagen miniprep kit	Plasmid	233	233	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)			53	-18.5	536			G233	DD1388, DD1395	no result, no result
1209	O233-4	O233		09-24	13:00	Maggie	Growth	913C3	Qiagen miniprep kit	Plasmid	233	233	pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)			41	0.0	537			G233
1210	O262	O262		09-24	13:00	Maggie	Growth	917C2	Qiagen miniprep kit	Plasmid	262	262	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)			161	-6.0	538			G262	DD1389, DD139628HE03, 28HE04	no result, no resultok, ok
1211	O262-2	O262		09-24	13:00	Maggie	Growth	917C3	Qiagen miniprep kit	Plasmid	262	262	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)			151	0.0	539			G262
1212	O262-3	O262		09-24	13:00	Maggie	Growth	917C4	Qiagen miniprep kit	Plasmid	262	262	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)			148	0.0	540			G262
1213	O262-4	O262		09-24	13:00	Maggie	Growth	917C5	Qiagen miniprep kit	Plasmid	262	262	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)			208	0.0	541			G262	DD1390, DD1397	no result, no result
1214	P362	P362		09-24	15:00	Caro	Gel	369-2,369-3	Gel extraction	PP	362		pSBX1K3-BackboneH6			115	0.0
1215	P363	P363		09-24	15:00	Caro	Gel	369-4,369-5	Gel extraction	PP	363		SspDnaXc-sfGFP^C-H6			104	0.0
1216	P364	P364		09-24	15:00	Caro	Gel	369-6, 369-7	Gel extraction	PP	364		SspDnaXc*-sfGFP^C-H6			117	0.0
1217	P365	P365		09-24	15:00	Caro	Gel	370-1, 370-2	Gel extraction	PP	365		H6-sfGFP^N-DnaX^N-H6 PCR Product			29	0.0
1218	P366	P366		09-24	15:00	Caro	Gel	370-3, 370-4	Gel extraction	PP	366		H6-sfGFP^N-NpuDnaE^N-H6 C->G PCR Product			56	0.0
1219	P367	P367		09-24	15:00	Caro	Gel	370-6, 370-7	Gel extraction	PP	367		DnaX-S11N_H6			19	0.0
1220	P368	P368		09-25	15:00	Caro	Gel	383-2, 383-3	Gel extraction	PP	368		LOV-6-SspDnaXC			69	0.0
1221	P369	P369		09-25	15:00	Caro	Gel	383-4, 383-5	Gel extraction	PP	369		LOV-12-SspDnaXC			34	0.0
1222	P370	P370		09-25	15:00	Caro	Gel	384-1, 384-2, 384-4	Gel extraction	PP	370		LOV_H6_BsaIIasB			64	0.0
1223	P371	P371		09-25	15:00	Caro	Gel	372-2, 372-3	Gel extraction	PP	371		sfGFPC_SspDnaXC_H6			111	0.0
1224	P372	P372		09-25	15:00	Caro	Gel	373-2, 373-3	Gel extraction	PP	372		sfGFPC_BsaIIasB_H6			70	0.0
1225	O266	O266	-	09-24	12:00	Anna	Extern	Flo		Plasmid	266	266	pLentiCRISPR	H2O	886.0	1	886.0
1226	O220-5	O220		09-24	15:40		Purification	O220-2	Dilution	Plasmid	220	220	pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T	H2O	420.0	1	408.0
1227	O224-3	O224		09-24	15:40		Purification	O224	Dilution	Plasmid	224	224	pSBX4C5-0GateSspDnaX-S11N_mRFP1-168	H2O	472.0	1	468.0
1228	P382	P382	-	09-24	17:30	Anna	Gel	375-2	Gel extraction	PP	382		pLentiCRISPR PCR Product	H2O	20.0	23	0.0
1229	O260	O260		09-24	18:10	Jakob	Growth	925C1	Qiagen miniprep kit	Plasmid	260	260	pSBX1K3-BBa_I13507-GSGIDECHT	H2O	30.0		30.0				p1640	BF8295	insertion, caused by mutated template that was not checked correctly by Jan :-P
1230	O260-2	O260		09-24	18:10	Jakob	Growth	925C2	Qiagen miniprep kit	Plasmid	260	260	pSBX1K3-BBa_I13507-GSGIDECHT	H2O	30.0		30.0				p1640	BF8296	insertion Jan
1231	O261	O261		09-24	18:10	Jakob	Growth	926C1	Qiagen miniprep kit	Plasmid	261	261	pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha	H2O	30.0		30.0				p1641	BF8297	insertion
1232	O261-2	O261		09-24	18:10	Jakob	Growth	926C2	Qiagen miniprep kit	Plasmid	261	261	pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha	H2O	30.0		30.0				p1641	DD1398	insertion
1233	O260-3	O260		09-24	18:10	Jakob	Growth	927C1	Qiagen miniprep kit	Plasmid	260	260	pSBX1K3-BBa_I13507-GSGIDECHT	H2O	30.0		30.0				P344	DD1399	insertion
1234	O260-4	O260		09-24	18:10	Jakob	Growth	927C2	Qiagen miniprep kit	Plasmid	260	260	pSBX1K3-BBa_I13507-GSGIDECHT	H2O	30.0		30.0				P344	DD1400	insertion
1235	O255	O255		09-24	18:10	Jakob	Growth	897C1	Qiagen miniprep kit	Plasmid	255	255	pSBX4C5-0GateSspDnaX-coex_mRFP168/169	H2O	30.0		30.0				S255	DD1401, BI2146	no , no
1236	O257	O257		09-24	18:10	Jakob	Growth	899C1	Qiagen miniprep kit	Plasmid	257	257	pSBX4C5-0GateTerDnaE3-coex_mRFP168/169	H2O	30.0		30.0				S257	DD1402, BI2194	no , no
1237	O259	O259		09-24	18:10	Jakob	Growth	901C1	Qiagen miniprep kit	Plasmid	259	259	pSBX4C5-1GateNpuDnaE_mutated_coex	H2O	30.0		30.0				S259	DD1403, BI2195	no , no
1238	O255-2	O255		09-24	18:10	Jakob	Growth	902C1	Qiagen miniprep kit	Plasmid	255	255	pSBX4C5-0GateSspDnaX-coex_mRFP168/169	H2O	30.0		30.0				S255#2	DD1404, DD1406	no , no
1239	O257-2	O257		09-24	18:10	Jakob	Growth	903C1	Qiagen miniprep kit	Plasmid	257	257	pSBX4C5-0GateTerDnaE3-coex_mRFP168/169	H2O	30.0		30.0				S257#2	DD1405, DD1407	no , no
1240	P345	P345		09-24	20:00	Jakob	Gel	376-2-1, 376-3-1	Gel extraction	PP	345		SspDnaXN_H6	H2O	30.0	6	30.0
1241	P346	P346		09-24	20:00	Jakob	Gel	376-4-1, 376-5-1	Gel extraction	PP	346		mRFP2-168_H6_SspDnaXN	H2O	30.0	105	30.0
1242	P347	P347		09-24	20:00	Jakob	Gel	376-6-1, 376-7-1	Gel extraction	PP	347		mRFP2-168_H6_SspDnaXN*	H2O	30.0	67	30.0
1243	O274	O274	-	09-24	20:00	Anna	Extern	Julia	Dilution	Plasmid	274	274	PCDna3.1(-)	H2O		779	-1.9
1244	R338	R338	-	09-24	20:00	Anna	Gel	377-2	Gel extraction	Restriction	338		pLentiCRISPR PCR Product (BamHI/HindIII)	H2O	20.0	10	8.9
1245	R339	R339	-	09-24	20:00	Anna	Gel	377-3, 377-4	Gel extraction	Restriction	339		PCDna3.1(-) (BamHI/HindIII)	H2O	30.0	30	27.1
1246	P375	P375		09-25	0:38	Jan	Gel	379-1-1, 379-2-1	Gel extraction	PP	375		mRFP_BsmBI=GATG=IasB_BsaI=GGTG=IBmsB (C)	H2O	20.0	121	20.0
1247	P376	P376		09-25	0:38	Jan	Gel	379-3	Gel extraction	PP	376		mRFP_BsmBI=TGGT=IasB_BsaI=TAAT=IBmsB (N)	H2O	20.0	109	20.0
1248	P377	P377		09-25	0:38	Jan	Gel	379-5	Gel extraction	PP	377		LacI_VR_pSB*C3	H2O	20.0	83	20.0
1249	P374	P374		09-25	0:38	Jan	PCR	1644	PCR purification kit	PP	374	283	pSBX4C5-2GateNpuDnaEC_SN->AG noPstI (=O283)	H2O	20.0	160	20.0
1250	P325	P325		09-25	0:38	Jan	PCR	1681	PCR purification kit	PP	325		SspDnaE(N)_mRFP-NN_NC-ccdB	H2O	20.0	215	20.0
1251	P326	P326		09-25	0:38	Jan	PCR	1682	PCR purification kit	PP	326		SSpDnaE(C)_ccdB-CN_CC-mRFP	H2O	20.0	103	20.0
1252	P345-2	P345		09-25	1:30	Jakob	Gel	381-2-1, 381-3-1	Gel extraction	PP	345		SspDnaXN_H6	H2O	30.0	70	30.0
1253	O180-8	O180		09-25	12:30	Jakob	Growth	929C1	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	H2O	30.0	355	30.0		discarded		p1680	DD1408	no mutagenesis
1254	O180-9	O180		09-25	12:30	Jakob	Growth	929C2	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	H2O	30.0	196	30.0		discarded		p1680	DD1409	no mutagenesis
1255	O180-10	O180		09-25	12:30	Jakob	Growth	929C3	Qiagen miniprep kit	Plasmid	180	180	pSBX1K3-T7RBS-H6-sfGFP_T65C-H6	H2O	30.0	181	24.5				p1680	DD1410	OK, mutagenesis worked
1256	O223-4	O223		09-25	12:30	Jakob	Growth	924C1	Qiagen miniprep kit	Plasmid	223	223	pSBX4C5-0GateSspDnaX-S11C_mRFP169-225	H2O	30.0	450	30.0	128			O223-2
1257	R260-3	R260		09-25	15:30	MaxW	Oligos	200,201	Annealing	Restriction	260		flex-linker	H2O	500.0	2	500.0
1258	O251	O251		09-25	17:10	Silvan	Growth	931C2	Qiagen miniprep kit	Plasmid	251	251	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H6	EB	50.0	471	45.0	543			G251#2	28HD79	96bp deletion
1259	O251-2	O251		09-25	17:10	Silvan	Growth	931C3	Qiagen miniprep kit	Plasmid	251	251	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H6	EB	50.0		45.0	544			G251#2	28HD80	96bp deletion
1260	O252	O252		09-25	17:10	Silvan	Growth	932C1	Qiagen miniprep kit	Plasmid	252	252	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H6	EB	50.0	407	45.0	545			G252#2	28HD81	mixture
1261	O252-2	O252		09-25	17:10	Silvan	Growth	932C2	Qiagen miniprep kit	Plasmid	252	252	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H6	EB	50.0	509	45.0	546			G252#2	28HD82	probably ok, but short read; possibly G->V mutation in linker
1262	O253	O253		09-25	17:10	Silvan	Growth	933C1	Qiagen miniprep kit	Plasmid	253	253	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6	EB	50.0	487	45.0	547			G253#2	28HD83	ok
1263	O253-2	O253		09-25	17:10	Silvan	Growth	933C2	Qiagen miniprep kit	Plasmid	253	253	pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6	EB	50.0		45.0	548			G253#2	28HD84	63bp deletion
1264	O236	O236		09-25	17:10	Silvan	Growth	934C1	Qiagen miniprep kit	Plasmid	236	236	pSBX1K3-T7RBS-H6-Smt3-0ZSortA	EB	50.0	506	33.0	549			S236	28HD85, 28HD97, 28HD98	wrong primer, ok; start of the read (backbone) looks a bit strange, ok
1265	O236-2	O236		09-25	17:10	Silvan	Growth	934C2	Qiagen miniprep kit	Plasmid	236	236	pSBX1K3-T7RBS-H6-Smt3-0ZSortA	EB	50.0	420	45.0	550			S236	28HD86	wrong primer
1266	O238	O238		09-25	17:10	Silvan	Growth	935C1	Qiagen miniprep kit	Plasmid	238	238	pSBX1K3-T7RBS-H6-0ZSortA	EB	50.0	381	32.4	551			S238	28HD87, 28HD99, 28HE00	wrong primer, ok, ok
1267	O238-2	O238		09-25	17:10	Silvan	Growth	935C2	Qiagen miniprep kit	Plasmid	238	238	pSBX1K3-T7RBS-H6-0ZSortA	EB	50.0	331	45.0	552			S238	28HD88	wrong primer
1268	O125-11	O125		09-25	17:10	Silvan	Growth	936C1	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	313	40.0	553			S125	28HD89, 28HD90	ok, ok
1269	O125-12	O125		09-25	17:10	Silvan	Growth	936C2	Qiagen miniprep kit	Plasmid	125	125	pSB1C3-Smt3-2ZSortA-H6 (ccdB)	EB	50.0	333	50.0	554			S125
1270	O265	O265		09-25	17:55	Silvan	Growth	956C1	Qiagen miniprep kit	Plasmid	265	265	pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6	EB	30.0	61	22.5	555			G265	28HD91	single base deletion
1271	O265-2	O265		09-25	17:55	Silvan	Growth	956C2	Qiagen miniprep kit	Plasmid	265	265	pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6	EB	30.0	49	22.5	556			G265	28HD92	ok
1272	O265-3	O265		09-25	17:55	Silvan	Growth	956C3	Qiagen miniprep kit	Plasmid	265	265	pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6	EB	30.0	80	30.0	557			G265
1273	O250	O250		09-25	17:55	Silvan	Growth	957C1	Qiagen miniprep kit	Plasmid	250	250	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT)	EB	30.0	70	22.5	558			G250	28HD94	ok
1274	O250-2	O250		09-25	17:55	Silvan	Growth	957C2	Qiagen miniprep kit	Plasmid	250	250	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT)	EB	30.0		22.5	559			G250	28HD95	single silent mutation
1275	O254	O254		09-25	17:55	Silvan	Growth	958C1	Qiagen miniprep kit	Plasmid	254	254	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)	EB	30.0	58	22.5	560			G254	28HD96	sequence ok; signal intensity decreased after DNMT1
1276	O254-2	O254		09-25	17:55	Silvan	Growth	958C2	Qiagen miniprep kit	Plasmid	254	254	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)	EB	30.0	88	22.5	561			G254	28HD93	ok
1277	O254-3	O254		09-25	17:55	Silvan	Growth	958C3	Qiagen miniprep kit	Plasmid	254	254	pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)	EB	30.0		30.0	562			G254
1278	O65-14	O65		09-26	0:30	MaxW	Growth	835C2	Qiagen miniprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	50.0	122	-7.3				O65-8
1279	O65-15	O65		09-26	0:30	MaxW	Growth	835C2	Qiagen miniprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	50.0	66	4.8				O65-8
1280	O65-16	O65		09-26	0:30	MaxW	Growth	835C2	Qiagen miniprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	50.0	82	-11.1				O65-8
1281	O65-17	O65		09-26	0:30	MaxW	Growth	835C2	Qiagen miniprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	50.0	75	23.2				O65-8
1282	O65-18	O65		09-26	0:30	MaxW	Growth	835C2	Qiagen miniprep kit	Plasmid	65	65	pSB1C3-BBa_J04450	H2O	50.0	53	12.5				O65-8
1283	O263	O263		09-26	17:00	Lisa	Growth	946C1	Qiagen miniprep kit	Plasmid	263	263	pSB1C3-EX-BsaI-A-NN-IasB-OmpA	H2O	50.0	156	#DIV/0!	564			G263	28HE23, 28HE24	,
1284	O263-2	O263		09-26	17:00	Lisa	Growth	946C3	Qiagen miniprep kit	Plasmid	263	263	pSB1C3-EX-BsaI-A-NN-IasB-OmpA	H2O	50.0	232	#DIV/0!	565			G263	DD1411, DD141228HE25, 28HE26	, ok, ok
1285	R340	R340		09-26	20:00	MaxW	Gel	390-2, 390-3, 390-4, 390-5	Gel extraction	Restriction	340		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	30	14.9
1286	R342	R342		09-26	20:00	MaxW	Gel	390-6, 390-7, 390-8, 390-9	Gel extraction	Restriction	342		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	25	-6.9
1287	R344	R344		09-26	20:00	MaxW	Gel	390-10, 390-11, 390-12, 390-13	Gel extraction	Restriction	344		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	19	11.3
1288	R346	R346		09-26	20:00	MaxW	Gel	391-2, 391-3, 391-4, 391-5	Gel extraction	Restriction	346		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	15	9.1
1289	R348	R348		09-26	20:00	MaxW	Gel	391-6, 391-7, 391-8, 391-9	Gel extraction	Restriction	348		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	18	11.2
1290	P419	P419		09-26	21:00	Caro	Gel	394-2, 394-3	Gel extraction	PP	419		GFPn*1-157_H6_GATG_BsaI_KGG_GGCT=IasB	H2O	30.0	69	30.0
1291	P420	P420		09-26	21:00	Caro	Gel	394-4, 394-5	Gel extraction	PP	420		GFPc*158-238_NWC_CAAC_BsaI_H6_TAAT=IasB	H2O	30.0	63	30.0
1292	P421	P421		09-26	21:00	Caro	Gel	394-6, 394-7	Gel extraction	PP	421		mCherryN*1-168_BsaI=GATG-H6_KGG-GGCT=IasB	H2O	30.0	31	30.0
1293	P422	P422		09-26	21:00	Caro	Gel	394-9, 394-10	Gel extraction	PP	422		mCherryC*169-236_BsaI=AGGC-NWC_H6-TAAT=IasB	H2O	30.0	56	30.0
1294	P423	P423		09-26	21:00	Caro	Gel	394-11, 394-12	Gel extraction	PP	423		mRFPn*1-154_H6_BsaI=GATG_KGG-GGCT=IasB	H2O	30.0	139	30.0
1295	P424	P424		09-26	21:00	Caro	Gel	394-13, 394-14	Gel extraction	PP	424		mRFPn*1-168_H6_BsaI=GATG_KGG-GGCT=IasB	H2O	30.0	138	30.0
1296	P425	P425		09-26	21:00	Caro	Gel	395-2, 395-3	Gel extraction	PP	425		mRFPc*155-225_BsaI=AGGC-NWC_H6_TAAT=IasB	H2O	30.0	105	30.0
1297	P426	P426		09-26	21:00	Caro	Gel	395-4, 395-5	Gel extraction	PP	426		mRFPc*169-225_BsaI=AGGC-NWC_H6_TAAT=IasB	H2O	30.0	53	30.0
1298	O224-4	O224		09-26	21:00	Caro	Growth	950C2	Qiagen miniprep kit	Plasmid	224	224	pSBX4C5-0GateSspDnaX-S11N_mRFP1-168				0.0	566			O224
1299	O224-5	O224		09-26	21:00	Caro	Growth	950C3	Qiagen miniprep kit	Plasmid	224	224	pSBX4C5-0GateSspDnaX-S11N_mRFP1-168				0.0	567			O224
1300	O225-4	O225		09-26	21:00	Caro	Growth	951C2	Qiagen miniprep kit	Plasmid	225	225	pSBX4C5-0GateSspGyrB-S11C_mRFP169-225				0.0	568			O225-3
1301	O225-5	O225		09-26	21:00	Caro	Growth	951C3	Qiagen miniprep kit	Plasmid	225	225	pSBX4C5-0GateSspGyrB-S11C_mRFP169-225				0.0	569			O225-3
1302	O226-5	O226		09-26	21:00	Caro	Growth	952C2	Qiagen miniprep kit	Plasmid	226	226	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168				0.0	570			O226-4
1303	O226-6	O226		09-26	21:00	Caro	Growth	952C3	Qiagen miniprep kit	Plasmid	226	226	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168				0.0	571			O226-4
1304	O230-3	O230		09-26	21:00	Caro	Growth	953C1	Qiagen miniprep kit	Plasmid	230	230	pSBX4C5-0GateNpuDnaE_N_mRFP1-168				0.0	572			O230
1305	O223-5	O223		09-26	21:00	Caro	Growth	949C1	Qiagen miniprep kit	Plasmid	223	223	pSBX4C5-0GateSspDnaX-S11C_mRFP169-225			253	-4.0	573			O223-2
1306	R350	R350		09-26	21:30	MaxW	Oligos	411,412	Annealing	Restriction	350		univ#1long	H2O	500	2	500
1307	R351	R351		09-26	21:30	MaxW	Oligos	413,414	Annealing	Restriction	351		univ#1short	H2O	500	1	500
1308	R352	R352		09-26	21:30	MaxW	Oligos	415,416	Annealing	Restriction	352		univ#3	H2O	500	5	500
1309	R353	R353		09-26	21:30	MaxW	Oligos	417,418	Annealing	Restriction	353		bad2#2	H2O	500	1	500
1310	R354	R354		09-26	21:30	MaxW	Oligos	419,420	Annealing	Restriction	354		bad2#4	H2O	500	5	500
1311	R355	R355		09-26	21:30	MaxW	Oligos	421,422	Annealing	Restriction	355		bad3#2	H2O	500	2	500
1312	R356	R356		09-26	21:30	MaxW	Oligos	423,424	Annealing	Restriction	356		bad3#4	H2O	500	3	500
1313	R357	R357		09-26	21:30	MaxW	Oligos	425,426	Annealing	Restriction	357		bad4#2	H2O	500	5	500
1314	R358	R358		09-26	21:30	MaxW	Oligos	427,428	Annealing	Restriction	358		bad4#4	H2O	500	3	500
1315	R359	R359		09-26	21:30	MaxW	Oligos	429,430	Annealing	Restriction	359		bad4#5	H2O	500	4	500
1316	R360	R360		09-26	21:30	MaxW	Oligos	431,432	Annealing	Restriction	360		may2#2	H2O	500	4	500
1317	R361	R361		09-26	21:30	MaxW	Oligos	433,434	Annealing	Restriction	361		may3#2	H2O	500	5	500
1318	R362	R362		09-26	21:30	MaxW	Oligos	435,436	Annealing	Restriction	362		ord1#2	H2O	500	4	500
1319	R363	R363		09-26	21:30	MaxW	Oligos	437,438	Annealing	Restriction	363		ord2#2	H2O	500	4	500
1320	R364	R364		09-26	21:30	MaxW	Oligos	439,440	Annealing	Restriction	364		ord3#3	H2O	500	4	500
1321	R365	R365		09-26	21:30	MaxW	Oligos	441,442	Annealing	Restriction	365		ord4#2	H2O	500	3	500
1322	R366	R366		09-26	21:30	MaxW	Oligos	443,444	Annealing	Restriction	366		ord5#2	H2O	500	4	500
1323	R367	R367		09-26	21:30	MaxW	Oligos	445,446	Annealing	Restriction	367		ord6#2	H2O	500	4	500
1324	R368	R368		09-26	21:30	MaxW	Oligos	447,448	Annealing	Restriction	368		sgt3#2	H2O	500	3	500
1325	R369	R369		09-26	21:30	MaxW	Oligos	449,450	Annealing	Restriction	369		sgt4#2	H2O	500	4	500
1326	R370	R370		09-26	21:30	MaxW	Oligos	451,452	Annealing	Restriction	370		sho3#2	H2O	500	3	500
1327	O191-4	O191		09-26	21:12	Jan	Growth	857C2-2	Qiagen miniprep kit	Plasmid	191	191	pSBX4C5-1GateNpuDnaE(C)*-H6	H2O	50.0	492	48.0				G191#3
1328	R371	R371		09-26	21:30	MaxW	Oligos	453,454	Annealing	Restriction	371		rigid_RGT	H2O	500.0	4	500.0
1329	O23-4	O23				Anna	Purification	O23	Dilution	Plasmid	23	23	pRSFDuet-1-mDNMT1(731-1602)				0.0
1330	O23-5	O23				Anna	Purification	O23	Dilution	Plasmid	23	23	pRSFDuet-1-mDNMT1(731-1602)				0.0
1331	P373	P373		09-27	7:16	Jan	PCR	1643	PCR purification kit	PP	373	316	pSBX4C5-1GateNpuDnaEC_SN->AG_H6 noPstI (=316)	H2O	20.0		20.0
1332	O284	O284		09-27	0:30	MaxW	Growth	965C1	Qiagen miniprep kit	Plasmid	284	284	pSBX1K3-2Zv2Npu:LaLys+bad1	H2O	50.0	266	47.5	574			G284	28HE49	no insert
1333	O284-2	O284		09-27	0:30	MaxW	Growth	965C2	Qiagen miniprep kit	Plasmid	284	284	pSBX1K3-2Zv2Npu:LaLys+bad1	H2O	50.0	208	50.0	575			G284
1334	O285	O285		09-27	0:30	MaxW	Growth	966C1	Qiagen miniprep kit	Plasmid	285	285	pSBX1K3-2Zv2Npu:LaLys+may1	H2O	50.0	191	47.5	576			G285	28HE50	ok
1335	O285-2	O285		09-27	0:30	MaxW	Growth	966C2	Qiagen miniprep kit	Plasmid	285	285	pSBX1K3-2Zv2Npu:LaLys+may1	H2O	50.0	234	50.0	577			G285
1336	O286	O286		09-27	0:30	MaxW	Growth	967C1	Qiagen miniprep kit	Plasmid	286	286	pSBX1K3-2Zv2Npu:LaLys+sgt1	H2O	50.0	200	47.5	578			G286	28HE51	no insert
1337	O286-2	O286		09-27	0:30	MaxW	Growth	967C2	Qiagen miniprep kit	Plasmid	286	286	pSBX1K3-2Zv2Npu:LaLys+sgt1	H2O	50.0	178	50.0	579			G286
1338	O287	O287		09-27	0:30	MaxW	Growth	968C1	Qiagen miniprep kit	Plasmid	287	287	pSBX1K3-2Zv2Npu:LaLys+sgt2	H2O	50.0	224	47.5	580			G287	28HE52	ok
1339	O287-2	O287		09-27	0:30	MaxW	Growth	968C2	Qiagen miniprep kit	Plasmid	287	287	pSBX1K3-2Zv2Npu:LaLys+sgt2	H2O	50.0	220	50.0	581			G287
1340	O288	O288		09-27	0:30	MaxW	Growth	969C1	Qiagen miniprep kit	Plasmid	288	288	pSBX1K3-2Zv2Npu:LaLys+sho1	H2O	50.0	252	47.5	582			G288	28HE53	ok
1341	O288-2	O288		09-27	0:30	MaxW	Growth	969C2	Qiagen miniprep kit	Plasmid	288	288	pSBX1K3-2Zv2Npu:LaLys+sho1	H2O	50.0	235	50.0	583			G288
1342	O289	O289		09-27	0:30	MaxW	Growth	970C1	Qiagen miniprep kit	Plasmid	289	289	pSBX1K3-2Zv2Npu:LaLys+sho2	H2O	50.0	257	47.5	584			G289	28HE54	ok
1343	O289-2	O289		09-27	0:30	MaxW	Growth	970C2	Qiagen miniprep kit	Plasmid	289	289	pSBX1K3-2Zv2Npu:LaLys+sho2	H2O	50.0	197	50.0	585			G289
1344	O290	O290		09-27	0:30	MaxW	Growth	971C1	Qiagen miniprep kit	Plasmid	290	290	pSBX1K3-2Zv2Npu:LaLys+flexlinker	H2O	50.0	229	47.5	586			G290	28HE55	ok
1345	O290-2	O290		09-27	0:30	MaxW	Growth	971C2	Qiagen miniprep kit	Plasmid	290	290	pSBX1K3-2Zv2Npu:LaLys+flexlinker	H2O	50.0	220	50.0	587			G290
1346	O283	O283		09-28	2:24	Jan	Growth	961C2	Qiagen miniprep kit	Plasmid	283	283	pSBX4C5-2GateNpu*(C)_v2	H2O	50.0	179	49.0	591			P374
1347	O283-2	O283		09-28	2:24	Jan	Growth	961C3	Qiagen miniprep kit	Plasmid	283	283	pSBX4C5-2GateNpu*(C)_v2	H2O	50.0	154	49.0	592			P374
1348	O283-3	O283		09-28	2:24	Jan	Growth	961C4	Qiagen miniprep kit	Plasmid	283	283	pSBX4C5-2GateNpu*(C)_v2		50.0	209	49.0	593			P374
1349	P362-2	P362		09-28	15:00	Caro	PCR	1852	PCR purification kit	PP	362		pSBX1K3-BackboneH6	H2O	30.0	23	30.0
1350	P72-5	P72		09-28	18:00	Lisa	PCR	1853	PCR purification kit	PP	72		pSBX4C5_mRFP-BsaI_BsmBI-ccdB_C		30.0	106	30.0
1351	P71-5	P71		09-28	18:00	Lisa	PCR	1855	PCR purification kit	PP	71		pSBX4C5_ccdB-BsmBI_BsaI-mRFP_N		30.0	84	30.0
1352	O291	O291		09-29	16:00	MaxW	Growth	1006C1	Qiagen miniprep kit	Plasmid	291	291	pSBX1K3-2Zv2Npu:LaLys+bad2	H2O	50.0	200	47.5	636			G291	28HE56	bad
1353	O291-2	O291		09-29	16:00	MaxW	Growth	1006C2	Qiagen miniprep kit	Plasmid	291	291	pSBX1K3-2Zv2Npu:LaLys+bad2	H2O	50.0	200	50.0	637			G291
1354	O292	O292		09-29	16:00	MaxW	Growth	1007C1	Qiagen miniprep kit	Plasmid	292	292	pSBX1K3-2Zv2Npu:LaLys+bad3	H2O	50.0	200	47.5	638			G292	28HE57	bad
1355	O292-2	O292		09-29	16:00	MaxW	Growth	1007C2	Qiagen miniprep kit	Plasmid	292	292	pSBX1K3-2Zv2Npu:LaLys+bad3	H2O	50.0	200	50.0	639			G292
1356	O293	O293		09-29	16:00	MaxW	Growth	1008C1	Qiagen miniprep kit	Plasmid	293	293	pSBX1K3-2Zv2Npu:LaLys+bad4	H2O	50.0	200	47.5	640			G293	28HE58	bad
1357	O293-2	O293		09-29	16:00	MaxW	Growth	1008C2	Qiagen miniprep kit	Plasmid	293	293	pSBX1K3-2Zv2Npu:LaLys+bad4	H2O	50.0	200	50.0	641			G293
1358	O294	O294		09-29	16:00	MaxW	Growth	1009C1	Qiagen miniprep kit	Plasmid	294	294	pSBX1K3-2Zv2Npu:LaLys+may2	H2O	50.0	200	47.5	642			G294	28HE59	no insert
1359	O294-2	O294		09-29	16:00	MaxW	Growth	1009C2	Qiagen miniprep kit	Plasmid	294	294	pSBX1K3-2Zv2Npu:LaLys+may2	H2O	50.0	200	50.0	643			G294
1360	O295	O295		09-29	16:00	MaxW	Growth	1010C1	Qiagen miniprep kit	Plasmid	295	295	pSBX1K3-2Zv2Npu:LaLys+may3	H2O	50.0	200	47.5	644			G295	28HE60	half insert
1361	O295-2	O295		09-29	16:00	MaxW	Growth	1010C2	Qiagen miniprep kit	Plasmid	295	295	pSBX1K3-2Zv2Npu:LaLys+may3	H2O	50.0	200	50.0	645			G295
1362	O296	O296		09-29	16:00	MaxW	Growth	1011C1	Qiagen miniprep kit	Plasmid	296	296	pSBX1K3-2Zv2Npu:LaLys+ord1	H2O	50.0	200	50.0	646			G296
1363	O296-2	O296		09-29	16:00	MaxW	Growth	1011C2	Qiagen miniprep kit	Plasmid	296	296	pSBX1K3-2Zv2Npu:LaLys+ord1	H2O	50.0	200	47.5	647			G296	28HE61	ok
1364	O297	O297		09-29	16:00	MaxW	Growth	1012C1	Qiagen miniprep kit	Plasmid	297	297	pSBX1K3-2Zv2Npu:LaLys+ord2	H2O	50.0	200	47.5	648			G297	28HE62	deletions
1365	O297-2	O297		09-29	16:00	MaxW	Growth	1012C2	Qiagen miniprep kit	Plasmid	297	297	pSBX1K3-2Zv2Npu:LaLys+ord2	H2O	50.0	200	50.0	649			G297
1366	O298	O298		09-29	16:00	MaxW	Growth	1013C1	Qiagen miniprep kit	Plasmid	298	298	pSBX1K3-2Zv2Npu:LaLys+ord3	H2O	50.0	200	47.5	650			G298	28HE63	ok
1367	O298-2	O298		09-29	16:00	MaxW	Growth	1013C2	Qiagen miniprep kit	Plasmid	298	298	pSBX1K3-2Zv2Npu:LaLys+ord3	H2O	50.0	200	50.0	651			G298
1368	O299	O299		09-29	16:00	MaxW	Growth	1014C1	Qiagen miniprep kit	Plasmid	299	299	pSBX1K3-2Zv2Npu:LaLys+ord4	H2O	50.0	200	47.5	652			G299	28HE64	half insert
1369	O299-2	O299		09-29	16:00	MaxW	Growth	1014C2	Qiagen miniprep kit	Plasmid	299	299	pSBX1K3-2Zv2Npu:LaLys+ord4	H2O	50.0	200	50.0	653			G299
1370	O300	O300		09-29	16:00	MaxW	Growth	1015C1	Qiagen miniprep kit	Plasmid	300	300	pSBX1K3-2Zv2Npu:LaLys+ord5	H2O	50.0	200	47.5	654			G300	28HE65	no insert
1371	O300-2	O300		09-29	16:00	MaxW	Growth	1015C2	Qiagen miniprep kit	Plasmid	300	300	pSBX1K3-2Zv2Npu:LaLys+ord5	H2O	50.0	200	50.0	655			G300
1372	O301	O301		09-29	16:00	MaxW	Growth	1016C2	Qiagen miniprep kit	Plasmid	301	301	pSBX1K3-2Zv2Npu:LaLys+ord6	H2O	50.0	200	47.5	656			G301	28HE66	no insert
1373	O301-2	O301		09-29	16:00	MaxW	Growth	1016C3	Qiagen miniprep kit	Plasmid	301	301	pSBX1K3-2Zv2Npu:LaLys+ord6	H2O	50.0	200	50.0	657			G301
1374	O302	O302		09-29	16:00	MaxW	Growth	1017C1	Qiagen miniprep kit	Plasmid	302	302	pSBX1K3-2Zv2Npu:LaLys+sgt3	H2O	50.0	200	47.5	658			G302	28HE67	no insert
1375	O302-2	O302		09-29	16:00	MaxW	Growth	1017C2	Qiagen miniprep kit	Plasmid	302	302	pSBX1K3-2Zv2Npu:LaLys+sgt3	H2O	50.0	200	50.0	659			G302
1376	O303	O303		09-29	16:00	MaxW	Growth	1018C1	Qiagen miniprep kit	Plasmid	303	303	pSBX1K3-2Zv2Npu:LaLys+sgt4	H2O	50.0	200	47.5	660			G303	28HE68	no insert
1377	O303-2	O303		09-29	16:00	MaxW	Growth	1018C2	Qiagen miniprep kit	Plasmid	303	303	pSBX1K3-2Zv2Npu:LaLys+sgt4	H2O	50.0	200	50.0	661			G303
1378	O304	O304		09-29	16:00	MaxW	Growth	1019C1	Qiagen miniprep kit	Plasmid	304	304	pSBX1K3-2Zv2Npu:LaLys+sho3	H2O	50.0	200	47.5	662			G304	28HE69	no insert
1379	O304-2	O304		09-29	16:00	MaxW	Growth	1019C2	Qiagen miniprep kit	Plasmid	304	304	pSBX1K3-2Zv2Npu:LaLys+sho3	H2O	50.0	200	50.0	663			G304
1380	O305	O305		09-29	16:00	MaxW	Growth	1020C1	Qiagen miniprep kit	Plasmid	305	305	pSBX1K3-2Zv2Npu:LaLys+rigid_RGT	H2O	50.0	200	47.5	664			G305	28HE70	insertions
1381	O305-2	O305		09-29	16:00	MaxW	Growth	1020C2	Qiagen miniprep kit	Plasmid	305	305	pSBX1K3-2Zv2Npu:LaLys+rigid_RGT	H2O	50.0	200	50.0	665			G305
1382	O192-3	O192		09-29	11:30	Caro	Growth	856C2-1	Qiagen miniprep kit	Plasmid	192	192	pSBX4C5-1GateNpuDnaE(N)*-H6	H2O	30.0	278	26.4				G192#3
1383	P175-3	P175		09-29	2:52	Jan	Gel	403-4,403-5	Gel extraction	PP	175		pSB1C3-BBa_K741002_MT_SspDnaBN	EB	20.0	22	20.0
1384	P175-4	P175		09-29	2:52	Jan	Gel	403-10,403-11	Gel extraction	PP	175		pSB1C3-BBa_K741002_MT_SspDnaBN	EB	20.0	48	20.0
1385	P187-2	P187		09-29	6:00	Jan	Oligo	185,193	Mix	PP	187	187	CPEC_GFP_Insert_oligo	H2O		260	0.0
1386	O278	O278		09-29	11:30	Jakob	Growth	983C1	Qiagen miniprep kit	Plasmid	278	278	pSBX1K3-DnaXN-sfGFPn-H6	H2O	30.0	387	22.3	595			A278	DD1413	ok one additional His
1387	O278-2	O278		09-29	11:30	Jakob	Growth	983C2	Qiagen miniprep kit	Plasmid	278	278	pSBX1K3-DnaXN-sfGFPn-H6	H2O	30.0	402	30.0	596			A278	DD1414	ok two additional His
1388	O278-3	O278		09-29	11:30	Jakob	Growth	983C3	Qiagen miniprep kit	Plasmid	278	278	pSBX1K3-DnaXN-sfGFPn-H6	H2O	30.0		30.0	597			A278
1389	O277	O277		09-29	11:30	Jakob	Growth	984C1	Qiagen miniprep kit	Plasmid	277	277	pSBX1K3-DnaXN*-sfGFPn-H6	H2O	30.0		30.0	598			A277	DD1415	ok one additional His+
1390	O277-2	O277		09-29	11:30	Jakob	Growth	984C2	Qiagen miniprep kit	Plasmid	277	277	pSBX1K3-DnaXN*-sfGFPn-H6	H2O	30.0	210	25.2	599			A277	DD1416	okay
1391	O277-3	O277		09-29	11:30	Jakob	Growth	984C3	Qiagen miniprep kit	Plasmid	277	277	pSBX1K3-DnaXN*-sfGFPn-H6	H2O	30.0		30.0	600			A277
1392	O276	O276		09-29	11:30	Jakob	Growth	985C1	Qiagen miniprep kit	Plasmid	276	276	pSBX1K3-SspDnaXc-sfGFP^C-H6	H2O	30.0	117	21.5	601			A276	DD1417	ok? two additional His
1393	O276-2	O276		09-29	11:30	Jakob	Growth	985C2	Qiagen miniprep kit	Plasmid	276	276	pSBX1K3-SspDnaXc-sfGFP^C-H6	H2O	30.0		30.0	602			A276	DD1418	do not use! bad mutations
1394	O276-3	O276		09-29	11:30	Jakob	Growth	985C3	Qiagen miniprep kit	Plasmid	276	276	pSBX1K3-SspDnaXc-sfGFP^C-H6	H2O	30.0		30.0	603			A276
1395	O279	O279		09-29	11:30	Jakob	Growth	987C1	Qiagen miniprep kit	Plasmid	279	279	pSBX1K3-LOV-BsaIIasB-sfGFPc	H2O	30.0	462	27.8	604			A279	DD1419, DD1435	could be O281?? checking with next sequencing, changed sequencing or construct, additional His, could be O281?? checking with next sequencing
1396	O279-2	O279		09-29	11:30	Jakob	Growth	987C2	Qiagen miniprep kit	Plasmid	279	279	pSBX1K3-LOV-BsaIIasB-sfGFPc	H2O	30.0		30.0	605			A279	DD1420, DD1436	could be O281?? checking with next sequencing, changed sequencing or construct, additional His, could be O281?? checking with next sequencing, changed sequencing or construct, additional His
1397	O279-3	O279		09-29	11:30	Jakob	Growth	987C3	Qiagen miniprep kit	Plasmid	279	279	pSBX1K3-LOV-BsaIIasB-sfGFPc	H2O	30.0		30.0	606			A279
1398	O280	O280		09-29	11:30	Jakob	Growth	988C1	Qiagen miniprep kit	Plasmid	280	280	pSBX1K3-LOV-12-DnaXC-sfGFPc	H2O	30.0		30.0	607			A280	DD1421, DD1437	no LOV domain, no LOV domain
1399	O280-2	O280		09-29	11:30	Jakob	Growth	988C2	Qiagen miniprep kit	Plasmid	280	280	pSBX1K3-LOV-12-DnaXC-sfGFPc	H2O	30.0		30.0	608			A280	DD1422, DD1438	one additional His, frame shift in the end, one additional His, frame shift in the end
1400	O280-3	O280		09-29	11:30	Jakob	Growth	988C3	Qiagen miniprep kit	Plasmid	280	280	pSBX1K3-LOV-12-DnaXC-sfGFPc	H2O	30.0	503	26.2	609			A280	28HE14, 28HE18	ok, good, ok, good
1401	O281	O281		09-29	11:30	Jakob	Growth	989C1	Qiagen miniprep kit	Plasmid	281	281	pSBX1K3-LOV-6-DnaXC-sfGFPc	H2O	30.0	472	27.9	610			A281	DD1423, DD1439	could be O279?? checking with next sequencing, changed sequencing or construct, additional His, could be O279?? checking with next sequencing, changed sequencing or construct, additional His
1402	O281-2	O281		09-29	11:30	Jakob	Growth	989C2	Qiagen miniprep kit	Plasmid	281	281	pSBX1K3-LOV-6-DnaXC-sfGFPc	H2O	30.0		30.0	613			A267	DD1424, DD1440	could be O279?? checking with next sequencing, changed sequencing or construct, additional His, could be O279?? checking with next sequencing, changed sequencing or construct, additional His
1403	O281-3	O281		09-29	11:30	Jakob	Growth	989C3	Qiagen miniprep kit	Plasmid	281	281	pSBX1K3-LOV-6-DnaXC-sfGFPc	H2O	30.0		30.0	614			A267
1404	O267	O267		09-29	11:30	Jakob	Growth	990C1	Qiagen miniprep kit	Plasmid	267	267	pSBX1K3-H6-mRFPn-DnaXN-H6	H2O	30.0		30.0	615			A267	DD1425	bad read at the beginning, probably frame shift mutations in between
1405	O267-2	O267		09-29	11:30	Jakob	Growth	990C2	Qiagen miniprep kit	Plasmid	267	267	pSBX1K3-H6-mRFPn-DnaXN-H6	H2O	30.0		30.0	616			A268	DD1426	turned out to be pSBX1K3-sfGFPn-NpuDnaEN
1406	O267-3	O267		09-29	11:30	Jakob	Growth	990C3	Qiagen miniprep kit	Plasmid	267	267	pSBX1K3-H6-mRFPn-DnaXN-H6	H2O	30.0	374	20.8	617			A268	28HE15	ok, good
1407	O268	O268		09-29	11:30	Jakob	Growth	991C1	Qiagen miniprep kit	Plasmid	268	268	pSBX1K3-H6-mRFPn-DnaXN*-H6	H2O	30.0		30.0	618			A268	DD1427	Intein DnaXN insert not included
1408	O268-2	O268		09-29	11:30	Jakob	Growth	991C2	Qiagen miniprep kit	Plasmid	268	268	pSBX1K3-H6-mRFPn-DnaXN*-H6	H2O	30.0		30.0	619			A269	DD1428	Intein DnaXN insert not included
1409	O268-3	O268		09-29	11:30	Jakob	Growth	991C3	Qiagen miniprep kit	Plasmid	268	268	pSBX1K3-H6-mRFPn-DnaXN*-H6	H2O	30.0	516	27.2	620			A269	28HE16	ok, good
1410	O269	O269		09-29	11:30	Jakob	Growth	992C1	Qiagen miniprep kit	Plasmid	269	269	pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6	H2O	30.0		30.0	621			A269	DD1429, DD1441	frame shift between His,
1411	O269-2	O269		09-29	11:30	Jakob	Growth	992C2	Qiagen miniprep kit	Plasmid	269	269	pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6	H2O	30.0	256	26.1	622			A270	DD1430, DD1442	ok one additional His,
1412	O269-3	O269		09-29	11:30	Jakob	Growth	992C3	Qiagen miniprep kit	Plasmid	269	269	pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6	H2O	30.0		30.0	623			A270
1413	O270	O270		09-29	11:30	Jakob	Growth	993C1	Qiagen miniprep kit	Plasmid	270	270	pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6	H2O	30.0	260	26.2	624			A270	DD1431, DD1443	okay,
1414	O270-2	O270		09-29	11:30	Jakob	Growth	993C2	Qiagen miniprep kit	Plasmid	270	270	pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6	H2O	30.0		30.0	625			A271	DD1432, DD1444	okay, one point mutation in Lac Operator,
1415	O270-3	O270		09-29	11:30	Jakob	Growth	993C3	Qiagen miniprep kit	Plasmid	270	270	pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6	H2O	30.0		30.0	626			A271
1416	O271	O271		09-29	11:30	Jakob	Growth	994C1	Qiagen miniprep kit	Plasmid	271	271	pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6	H2O	30.0		30.0	627			A273	DD1433, DD1445	6 amino acids missing in helixintein construct,
1417	O271-2	O271		09-29	11:30	Jakob	Growth	994C2	Qiagen miniprep kit	Plasmid	271	271	pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6	H2O	30.0		30.0	628			A273	DD1434, DD1446	LOV domain missing,
1418	O273	O273		09-29	11:30	Jakob	Growth	996C1	Qiagen miniprep kit	Plasmid	273	273	pSBX1K3-H6-DnaXC*-mRFPc-H6	H2O	30.0	110	#N/A	629			A273	28HE71	is pSBX1K3-H6-DnaXC-mRFPc-H6, mutation H->Q ?, short read
1419	O273-2	O273		09-29	11:30	Jakob	Growth	996C2	Qiagen miniprep kit	Plasmid	273	273	pSBX1K3-H6-DnaXC*-mRFPc-H6	H2O	30.0		#N/A	628			A273	28HE72	bad read, end of mRFP okay
1420	O273-3	O273		09-29	11:30	Jakob	Growth	996C3	Qiagen miniprep kit	Plasmid	273	273	pSBX1K3-H6-DnaXC*-mRFPc-H6	H2O	30.0		#DIV/0!	629			A273	28HE17	many mutations
1421	O316	O316		09-29	11:23	Jan	Growth	1021C1	Qiagen miniprep kit	Plasmid	316	316	pSBX4C5-H6-1GateNpu*(C)_v2	H2O	30.0	395	29.0				P373
1422	O316-2	O316		09-29	11:23	Jan	Growth	1021C2	Qiagen miniprep kit	Plasmid	316	316	pSBX4C5-H6-1GateNpu*(C)_v2	H2O	30.0	262	29.0				P373
1423	O316-3	O316		09-29	11:23	Jan	Growth	1021C3	Qiagen miniprep kit	Plasmid	316	316	pSBX4C5-H6-1GateNpu*(C)_v2	H2O	30.0	264	29.0				P373
1424	O316-4	O316		09-29	11:23	Jan	Growth	1021C4	Qiagen miniprep kit	Plasmid	316	316	pSBX4C5-H6-1GateNpu*(C)_v2	H2O	30.0	230	29.0				P373
1425	O316-5	O316		09-29	11:23	Jan	Growth	1021C5	Qiagen miniprep kit	Plasmid	316	316	pSBX4C5-H6-1GateNpu*(C)_v2	H2O	30.0	366	29.0				P373
1426	O316-6	O316		09-29	11:23	Jan	Growth	1021C6	Qiagen miniprep kit	Plasmid	316	316	pSBX4C5-H6-1GateNpu*(C)_v2	H2O	30.0	355	#N/A				P373	28HE48	ok
1427	O273-4	O273		09-29	18:00	Caro	Growth	1028C1	Qiagen miniprep kit	Plasmid	273	273	pSBX1K3-H6-DnaXC*-mRFPc-H6	H2O	30.0		30.0				A273
1428	O273-5	O273		09-29	18:00	Caro	Growth	1028C2	Qiagen miniprep kit	Plasmid	273	273	pSBX1K3-H6-DnaXC*-mRFPc-H6	H2O	30.0		30.0				A273
1429	O273-6	O273		09-29	18:00	Caro	Growth	1028C4	Qiagen miniprep kit	Plasmid	273	273	pSBX1K3-H6-DnaXC*-mRFPc-H6	H2O	30.0		30.0				A273
1430	O275	O275		09-29	18:00	Caro	Growth	1029C1	Qiagen miniprep kit	Plasmid	275	275	pSBX1K3-SspDnaXc*-sfGFP^C-H6	H2O	30.0		#N/A				A275	28HE46	turned out to be SspDnaXN-sfGFPN, one additional His
1431	O275-2	O275		09-29	18:00	Caro	Growth	1029C3	Qiagen miniprep kit	Plasmid	275	275	pSBX1K3-SspDnaXc*-sfGFP^C-H6	H2O	30.0		#N/A				A275	28HE47	turned out to be SspDnaXN-sfGFPN, frame shift in front of His
1432	O275-3	O275		09-29	18:00	Caro	Growth	1029C4	Qiagen miniprep kit	Plasmid	275	275	pSBX1K3-SspDnaXc*-sfGFP^C-H6	H2O	30.0		#DIV/0!				A275	28HE20	no actually DnaXN-GFPn
1433	R402	R402		09-30	17:00	Lisa	Gel	408-2	Gel extraction	Restriction	402		pSBX4C5-2GateNpuDnaE(N) (E/P)	H2O	30.0		17.6
1434	R403	R403		09-30	17:00	Lisa	Gel	408-3	Gel extraction	Restriction	403		pSBX4C5-2GateNpuDnaE(C) (E/P)	H2O	30.0		21.1
1435	R384	R384		09-30	15:00	MaxW	Restriction	384	PCR purification kit	Restriction	384		pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 (E/P)	H2O	20.0	5	8.6
1436	R385	R385		09-30	15:00	MaxW	Restriction	385	PCR purification kit	Restriction	385		pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 (E/P)	H2O	20.0	4	8.4
1437	R386	R386		09-30	15:00	MaxW	Restriction	386	PCR purification kit	Restriction	386		pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 (E/P)	H2O	20.0	6	8.9
1438	R387	R387		09-30	15:00	MaxW	Restriction	387	PCR purification kit	Restriction	387		pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 (E/P)	H2O	20.0	8	9.4
1439	R388	R388		09-30	15:00	MaxW	Restriction	388	PCR purification kit	Restriction	388		pSBX1K3-2Zv2Npu:(LambdaLys-H6) (E/P)	H2O	20.0	9	9.3
1440	R389	R389		09-30	15:00	MaxW	Restriction	389	PCR purification kit	Restriction	389		pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) (E/P)	H2O	20.0	10	9.5
1441	R390	R390		09-30	15:00	MaxW	Restriction	390	PCR purification kit	Restriction	390		pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His (E/P)	H2O	20.0	10	9.5
1442	R391	R391		09-30	15:00	MaxW	Restriction	391	PCR purification kit	Restriction	391		pSBX1K3-2Zv2Npu:Xyla_circ_noHis (E/P)	H2O	20.0	19	11.4
1443	R392	R392		09-30	15:00	MaxW	Restriction	392	PCR purification kit	Restriction	392		pSBX1K3-1GateNpuDnaE(N) (E/P)	H2O	20.0	7	9.0
1444	R393	R393		09-30	15:00	MaxW	Restriction	393	PCR purification kit	Restriction	393		pSBX1K3-1GateNpuDnaE(C) (E/P)	H2O	20.0	8	9.3
1445	R394	R394		09-30	15:00	MaxW	Restriction	394	PCR purification kit	Restriction	394		pSBX1K3-2Zv2Npu:Xyla(H6)destrCext (E/P)	H2O	20.0	11	10.1
1446	R395	R395		09-30	15:00	MaxW	Restriction	395	PCR purification kit	Restriction	395		pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (E/P)	H2O	20.0	13	9.8
1447	R396	R396		09-30	15:00	MaxW	Restriction	396	PCR purification kit	Restriction	396		pSBX1K3-Xylanase (E/P)	H2O	20.0	4	8.6
1448	R397	R397		09-30	15:00	MaxW	Restriction	397	PCR purification kit	Restriction	397		pSBX1K3-LiLaLy(H6) (E/P)	H2O	20.0	12	10.7
1449	R398	R398		09-30	15:00	MaxW	Restriction	398	PCR purification kit	Restriction	398		pSBX1K3-XylaH6BB (E/P)	H2O	20.0	12	10.5
1450	R399	R399		09-30	15:00	MaxW	Restriction	399	PCR purification kit	Restriction	399		pSBX1K3-XylaH6extBB (E/P)	H2O	20.0	9	9.9
1451	O227-4	O227		09-30	18:00	Caro	Growth	1063C1	Qiagen miniprep kit	Plasmid	227	227	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225				0.0				G227#2
1452	O227-5	O227		09-30	18:00	Caro	Growth	1063C2	Qiagen miniprep kit	Plasmid	227	227	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225			73	#N/A	691			G227#2	28HE05	good, ok
1453	O227-6	O227		09-30	18:00	Caro	Growth	1063C3	Qiagen miniprep kit	Plasmid	227	227	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225				0.0				G227#2
1454	O308	O308		09-30	18:00	Caro	Growth	1064C1	Qiagen miniprep kit	Plasmid	308	308	pSBX4C5-0GateSspDnaX-S11C_mRFP169-225			130	#N/A				G308	28HE06	NpuDnaEC sequence in front of mRFPc not use
1455	O308-2	O308		09-30	18:00	Caro	Growth	1064C2	Qiagen miniprep kit	Plasmid	308	308	pSBX4C5-0GateSspDnaX-S11C_mRFP169-225				0.0				G308
1456	O308-3	O308		09-30	18:00	Caro	Growth	1064C3	Qiagen miniprep kit	Plasmid	308	308	pSBX4C5-0GateSspDnaX-S11C_mRFP169-225				0.0				G308
1457	O309	O309		09-30	18:00	Caro	Growth	1065C1	Qiagen miniprep kit	Plasmid	309	309	pSBX4C5-0GateSspDnaX-S11N_mRFP1-168				0.0				G309
1458	O309-2	O309		09-30	18:00	Caro	Growth	1065C2	Qiagen miniprep kit	Plasmid	309	309	pSBX4C5-0GateSspDnaX-S11N_mRFP1-168			130	#N/A				G309	28HE07	no DnaXN*
1459	O309-3	O309		09-30	18:00	Caro	Growth	1065C3	Qiagen miniprep kit	Plasmid	309	309	pSBX4C5-0GateSspDnaX-S11N_mRFP1-168				0.0				G309
1460	O310	O310		09-30	18:00	Caro	Growth	1066C1	Qiagen miniprep kit	Plasmid	310	310	pSBX4C5-0GateSspGyrB-S11C_mRFP169-225			133	-15.0	680	actually O308		G310	28HE08, 28HE21	ok good, but not 310, DnaXC* inside (O308), ok, good but is actually DnaXC*-mRFPc (O308)
1461	O310-2	O310		09-30	18:00	Caro	Growth	1066C2	Qiagen miniprep kit	Plasmid	310	310	pSBX4C5-0GateSspGyrB-S11C_mRFP169-225				0.0				G310
1462	O310-3	O310		09-30	18:00	Caro	Growth	1066C3	Qiagen miniprep kit	Plasmid	310	310	pSBX4C5-0GateSspGyrB-S11C_mRFP169-225				0.0				G310
1463	O311	O311		09-30	18:00	Caro	Growth	1067C1	Qiagen miniprep kit	Plasmid	311	311	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168			115	#DIV/0!	681	actually O309		G311	28HE09, 28HE35	ok good, but not 312 inside, DnaXN* inside (O309-2), ok, good, but actually DnaXN*-mRFPn (O309)
1464	O311-2	O311		09-30	18:00	Caro	Growth	1067C2	Qiagen miniprep kit	Plasmid	311	311	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168				0.0				G311
1465	O311-3	O311		09-30	18:00	Caro	Growth	1067C3	Qiagen miniprep kit	Plasmid	311	311	pSBX4C5-0GateSspGyrB-S11N_mRFP1-168				0.0				G311
1466	O312	O312		09-30	18:00	Caro	Growth	1068C1	Qiagen miniprep kit	Plasmid	312	312	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225				#DIV/0!	682	actually O310		G312	28HE10, 28HE36	ok good, but GyrBC* (O310) inside , ok , good, but actually GyrBC*-mRFPc (O310)
1467	O312-2	O312		09-30	18:00	Caro	Growth	1068C2	Qiagen miniprep kit	Plasmid	312	312	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225				0.0				G312
1468	O312-3	O312		09-30	18:00	Caro	Growth	1068C3	Qiagen miniprep kit	Plasmid	312	312	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225				0.0				G312
1469	O313	O313		09-30	18:00	Caro	Growth	1069C1	Qiagen miniprep kit	Plasmid	313	313	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168				#DIV/0!	683	actually O311		G313	28HE11, 28HE37	ok good, but GyrBN* (O311) inside, ok, good, but actually GyrBN*-mRFPn (O311)
1470	O313-2	O313		09-30	18:00	Caro	Growth	1069C2	Qiagen miniprep kit	Plasmid	313	313	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168				0.0				G313
1471	O313-3	O313		09-30	18:00	Caro	Growth	1069C3	Qiagen miniprep kit	Plasmid	313	313	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168				0.0				G313
1472	O314	O314		10-01	todo	Caro	Growth	1070C1	Qiagen miniprep kit	Plasmid	314	314	pSBX4C5-0GateNpuDnaE_C_mRFP169-225				#DIV/0!				G314	28HE33	not use, actually DnaXN- GFPn
1473	O314-2	O314		10-01	todo	Caro	Growth	1070C2	Qiagen miniprep kit	Plasmid	314	314	pSBX4C5-0GateNpuDnaE_C_mRFP169-225				#DIV/0!				G314	28HE34	not use, actually DnaXN- GFPn
1474	O314-3	O314		10-01	todo	Caro	Growth	1070C3	Qiagen miniprep kit	Plasmid	314	314	pSBX4C5-0GateNpuDnaE_C_mRFP169-225				0.0				G314
1475	O315	O315		09-30	todo	Caro	Growth	1071C1	Qiagen miniprep kit	Plasmid	315	315	pSBX4C5-0GateNpuDnaE_N_mRFP1-168				0.0				G315
1476	O315-2	O315		09-30	todo	Caro	Growth	1071C2	Qiagen miniprep kit	Plasmid	315	315	pSBX4C5-0GateNpuDnaE_N_mRFP1-168				0.0				G315
1477	O315-3	O315		09-30	todo	Caro	Growth	1071C3	Qiagen miniprep kit	Plasmid	315	315	pSBX4C5-0GateNpuDnaE_N_mRFP1-168				0.0				G315
1478	O132-4	O132		09-30	18:00	Lisa	Growth	1055C1	Qiagen miniprep kit	Plasmid	132	132	pSB1C3-Plac-GFP-IntN-MTS				-7.3				A132#4	28HE41, 28HE42	,
1479	O132-5	O132		09-30	18:00	Lisa	Growth	1055C2	Qiagen miniprep kit	Plasmid	132	132	pSB1C3-Plac-GFP-IntN-MTS				-7.3				A132#4	28HE40, 28HE39	,
1480	O132-6	O132		09-30	18:00	Lisa	Growth	1055C3	Qiagen miniprep kit	Plasmid	132	132	pSB1C3-Plac-GFP-IntN-MTS				0.0				A132#4
1481	O340	O340		09-30	20:40	Lisa	Ligation	S344	PCR purification kit	Plasmid	0	0	#N/A			12	0.0
1482	O341	O341		09-30	20:40	Lisa	Ligation	S345	PCR purification kit	Plasmid	0	0	#N/A			9	0.0
1483	R383	R383		09-30	22:10	MaxW	Gel	414-2	Gel extraction	Restriction	383		pSBX4C5-2Zv2Npu (E/P)	H2O	20.0	5	9.5
1484	P197-5	P197		10-01	0:05	Lisa	Gel	415-1,415-2	Gel extraction	PP	197		ccdB-pSBX4C5-mRFP(N)_	H2O	20.0	68	20.0
1485	P198-5	P198		10-01	0:05	Lisa	Gel	415-3,415-4	Gel extraction	PP	198		mRFP-pSBX4C5-ccdB-(C)_	H2O	20.0	34	20.0
1486	P478	P478		10-01	0:05	Lisa	Gel	415-5,415-6	Gel extraction	PP	478		ccdB-pSB1C3-mRFP(N)_	H2O	20.0	66	20.0
1487	P479	P479		10-01	0:05	Lisa	Gel	415-7,415-8	Gel extraction	PP	479		mRFP-pSB1C3-ccdB-(C)_	H2O	20.0	77	20.0
1488	O107-4	O107		10-01	14:00	MaxW	Growth	529C1-1	Qiagen miniprep kit	Plasmid	107	107	pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	H2O	30.0	241	25.9				A107
1489	P362-3	P362		10-01	16:00	Caro	Gel	417-2, 417-3	Gel extraction	PP	362		pSBX1K3-BackboneH6	H2O	30.0	8	30.0
1490	P362-4	P362		10-01	16:00	Caro	Gel	417-4, 417-5	Gel extraction	PP	362		pSBX1K3-BackboneH6	H2O	30.0	15	30.0
1491	P362-5	P362		10-01	16:00	Caro	Gel	417-6, 417-7	Gel extraction	PP	362		pSBX1K3-BackboneH6	H2O	30.0	9	30.0
1492	P480	P480		10-01	17:00	Lisa	Gel	418-2, 418-3	Gel extraction	PP	480		CBD_His-BsaI_BsaI-Next	H2O	30.0	34	30.0
1493	O117-6	O117		10-01	18:00	Caro	Growth	467C2-1	Qiagen miniprep kit	Plasmid	117	117	pSB1C3-BBa_K741002	H2O			#DIV/0!				D2013-2-17M	28HE12, 28HE13	,
1494	O364	O364		10-01	10	Jan	Extern	Konrad Herbst, pKH38.2		Plasmid	364	364	pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)	H2O	5.0	183	#DIV/0!					DD148128HE27, 28HE28	ok, but only mRFP sequenced, ok, but only mRFP sequenced
1495	O365	O365		10-01	10	Jan	Extern	Konrad Herbst, pKH39PstI.1		Plasmid	365	365	pSBX4C5-1GateNpuDnaE(N)-H6-1GateNpuDnaE(C)-H6	H2O	5.0	209	0.7					DD148228HE29, 28HE30	ok, but only mRFP sequenced, ok, but only mRFP sequenced
1496	O366	O366		10-01	10	Jan	Extern	Konrad Herbst, pKH39.2		Plasmid	366	366	pSBX4C5-1GateNpuDnaE(N)-H6-H6-1GateNpu(C)_v2	H2O	5.0	201	0.5					DD148328HE31, 28HE32	ok, but only mRFP sequenced, ok, but only mRFP sequenced
1497	O327	O327		10-01	18:37	Jan, Lisa	Growth	1072C1	Qiagen miniprep kit	Plasmid	327	327	pSBX4C5-1GateGp41-1(N)-H6	H2O	50.0	49	29.5	630			G327	DD1447	ok
1498	O327-2	O327		10-02	19:37	Jan, Lisa	Growth	1072C2	Qiagen miniprep kit	Plasmid	327	327	pSBX4C5-1GateGp41-1(N)-H6	H2O	50.0	95	39.5	674			G327	DD1448	will be repeated
1499	O328	O328		10-03	20:37	Jan, Lisa	Growth	1073C1	Qiagen miniprep kit	Plasmid	328	328	pSBX4C5-H6-1GateGp41-1(C)	H2O	50.0	130	42.3	631			G328	DD1449	ok
1500	O328-2	O328		10-04	21:37	Jan, Lisa	Growth	1073C2	Qiagen miniprep kit	Plasmid	328	328	pSBX4C5-H6-1GateGp41-1(C)	H2O	50.0	145	43.1	675			G328	DD1450	ok
1501	O329	O329		10-05	22:37	Jan, Lisa	Growth	1074C1	Qiagen miniprep kit	Plasmid	329	329	pSBX4C5-DoubleGateGp41-1(N)	H2O	50.0	79	37.3	632			G329	DD1451, DD1459	ok, not completely sequenced, ok, not completely sequenced
1502	O329-2	O329		10-06	23:37	Jan, Lisa	Growth	1074C2	Qiagen miniprep kit	Plasmid	329	329	pSBX4C5-DoubleGateGp41-1(N)	H2O	50.0	51	30.2	676			G329	DD1452, DD1460	bad sequenzing, bad sequenzing
1503	O330	O330		10-07	0:37	Jan, Lisa	Growth	1075C1	Qiagen miniprep kit	Plasmid	330	330	pSBX4C5-DoubleGateGp41-1(C)	H2O	50.0	101	40.1	633			G330	DD1453, DD1461	ok, not completely sequenced, ok, not completely sequenced
1504	O330-2	O330		10-08	1:37	Jan, Lisa	Growth	1075C2	Qiagen miniprep kit	Plasmid	330	330	pSBX4C5-DoubleGateGp41-1(C)	H2O	50.0	71	36.0	677			G330	DD1454, DD1462	insertion in the prefix, mutation in mRFP, poor sequenzing, but ok
1505	O331	O331		10-09	2:37	Jan, Lisa	Growth	1076C1	Qiagen miniprep kit	Plasmid	331	331	pSBX4C5-DoubleNpuDnaE(N)	H2O	50.0	69	35.4	634			G331	DD1455, DD1463	sequencing not long enough, poor sequenzing could be ok intein not sequenced
1506	O331-2	O331		10-10	3:37	Jan, Lisa	Growth	1076C2	Qiagen miniprep kit	Plasmid	331	331	pSBX4C5-DoubleNpuDnaE(N)	H2O	50.0	94	39.3	678			G331	DD1456, DD1464	sequencing not long enough, ok intein not sequenzed
1507	O332	O332		10-11	4:37	Jan, Lisa	Growth	1077C1	Qiagen miniprep kit	Plasmid	332	332	pSBX4C5-DoubleGateNpuDnaE(C)	H2O	50.0	62	33.7	635			G332	DD1457, DD1465	sequencing not long enough, intein not sequenced, short sequenzin but ok
1508	O332-2	O332		10-12	5:37	Jan, Lisa	Growth	1077C2	Qiagen miniprep kit	Plasmid	332	332	pSBX4C5-DoubleGateNpuDnaE(C)	H2O	50.0	67	35.1	679			G332	DD1458, DD1466	poor sequenzing, especially in the beginningg, but no indication that it was not ok, poor sequrnzing could be ok
1509	R404	R404		10-01	22:15	MaxW	Gel	419-2	Gel extraction	Restriction	404		pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 (E/P)	H2O	20.0	1	9.2
1510	R405	R405		10-01	22:15	MaxW	Gel	419-3	Gel extraction	Restriction	405		pSBX4C5-1Gate-NpuDnaE(N)-H6 (E/P)	H2O	20.0	10	13.8
1511	R406	R406		10-01	22:15	MaxW	Gel	419-4	Gel extraction	Restriction	406		pSBX4C5-H6-1GateNpuDnaE(C) (E/P)	H2O	20.0	6	11.5
1512	R407	R407		10-01	22:15	MaxW	Gel	419-5	Gel extraction	Restriction	407		pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 (E/P)	H2O	20.0	3	10.4
1513	R408	R408		10-01	22:15	MaxW	Gel	419-6	Gel extraction	Restriction	408		pSBX4C5-1GateSspDnaX-S11(C)-H6 (E/P)	H2O	20.0	7	12.2
1514	R409	R409		10-01	22:15	MaxW	Gel	419-7	Gel extraction	Restriction	409		pSBX4C5-1GateSspDnaX-S11N_H6 (E/P)	H2O	20.0	10	14.3
1515	R410	R410		10-01	22:15	MaxW	Gel	419-8	Gel extraction	Restriction	410		pSBX4C5-1GateSspGyrB-S11N_H6 (E/P)	H2O	20.0	11	14.4
1516	R411	R411		10-01	22:15	MaxW	Gel	419-9	Gel extraction	Restriction	411		pSBX4C5-1GateTerDnaE3-S11C_H6 (E/P)	H2O	20.0	7	12.9
1517	R412	R412		10-01	22:15	MaxW	Gel	419-10	Gel extraction	Restriction	412		pSBX4C5-1GateTerDnaE3-S11(N)-H6 (E/P)	H2O	20.0	10	14.3
1518	O272	O272		09-29	11:30	Caro	Growth	1037C1	Qiagen miniprep kit	Plasmid	272	272	pSBX1K3-H6-DnaXC-mRFPc-H6				#N/A	684			A272	28HE44, 28HE19	actually pSBX1K3-H6-DnaXC*-mRFPc-H6, construct or sequencing changed?, ok good, but actually O273
1519	O272-2	O272		09-29	11:30	Caro	Growth	1037C2	Qiagen miniprep kit	Plasmid	272	272	pSBX1K3-H6-DnaXC-mRFPc-H6				#N/A				A272	28HE45	actually pSBX1K3-H6-DnaXN-sfGFP-H6,do not use frameshift
1520	O272-3	O272		09-29	11:30	Caro	Growth	1037C3	Qiagen miniprep kit	Plasmid	272	272	pSBX1K3-H6-DnaXC-mRFPc-H6				0.0				A272
1521	O59-14	O59		02-10	16:00	Jakob	Purification	O59-9	Dilution	Plasmid	59	59	pSBX1K3-BBa_I13507	H2O	17.0		15.0
1522	O240	O240		02-10	15:00	Lisa	Growth	1078C1	Qiagen miniprep kit	Plasmid	240	240	pSBX4C5-2GateSspDnaB(C)			117	0.0	692			A240#5	DD1467	ok
1523	O240-2	O240		02-10	15:00	Lisa	Growth	1078C2	Qiagen miniprep kit	Plasmid	240	240	pSBX4C5-2GateSspDnaB(C)				0.0	693			A240#5
1524	O240-3	O240		02-10	15:00	Lisa	Growth	1078C3	Qiagen miniprep kit	Plasmid	240	240	pSBX4C5-2GateSspDnaB(C)			88	0.0	694			A240#5	DD1468	ok (maybe there is something in the beginning of mRFP)
1525	O241	O241		02-10	15:00	Lisa	Growth	1079C1	Qiagen miniprep kit	Plasmid	241	241	pSBX4C5-2GateSspDnaB(C)*				0.0	695			A241#5
1526	O241-2	O241		02-10	15:00	Lisa	Growth	1079C2	Qiagen miniprep kit	Plasmid	241	241	pSBX4C5-2GateSspDnaB(C)*			101	0.0	696			A241#5	DD1469	ok
1527	O241-3	O241		02-10	15:00	Lisa	Growth	1079C3	Qiagen miniprep kit	Plasmid	241	241	pSBX4C5-2GateSspDnaB(C)*			121	0.0	697			A241#5	DD1470	ok
1528	O242	O242		02-10	15:00	Lisa	Growth	1080C1	Qiagen miniprep kit	Plasmid	242	242	pSBX4C5-2GateSspDnaB(N)			127	0.0	698			A242#5	DD1471	perfect mRFP read, call GATC
1529	O242-2	O242		02-10	15:00	Lisa	Growth	1080C2	Qiagen miniprep kit	Plasmid	242	242	pSBX4C5-2GateSspDnaB(N)			92	0.0	699			A242#5	DD1472	perfect mRFP read, call GATC
1530	O242-3	O242		02-10	15:00	Lisa	Growth	1080C3	Qiagen miniprep kit	Plasmid	242	242	pSBX4C5-2GateSspDnaB(N)				0.0	700			A242#5
1531	O243	O243		02-10	15:00	Lisa	Growth	1081C1	Qiagen miniprep kit	Plasmid	243	243	pSBX4C5-2GateSspDnaB(N)*				0.0	701			A243#5
1532	O243-2	O243		02-10	15:00	Lisa	Growth	1081C2	Qiagen miniprep kit	Plasmid	243	243	pSBX4C5-2GateSspDnaB(N)*			68	0.0	702			A243#5	DD1473	perfect mRFP read, call GATC
1533	O243-3	O243		02-10	15:00	Lisa	Growth	1081C3	Qiagen miniprep kit	Plasmid	243	243	pSBX4C5-2GateSspDnaB(N)*			50	0.0	703			A243#5	DD1474	perfect mRFP read, call GATC
1534	O325	O325		02-10	15:00	Lisa	Growth	1082C1	Qiagen miniprep kit	Plasmid	325	325	pSBX4C5-2GateSspDnaE(N)			80	0.0	704			A325#2	DD1475,	perfect mRFP read, call GATC,
1535	O325-2	O325		02-10	15:00	Lisa	Growth	1082C2	Qiagen miniprep kit	Plasmid	325	325	pSBX4C5-2GateSspDnaE(N)			65	0.0	705			A325#2	DD1476,	perfect mRFP read, call GATC,
1536	O325-3	O325		02-10	15:00	Lisa	Growth	1082C3	Qiagen miniprep kit	Plasmid	325	325	pSBX4C5-2GateSspDnaE(N)				0.0	706			A325#2
1537	O326	O326		02-10	15:00	Lisa	Growth	1083C1	Qiagen miniprep kit	Plasmid	326	326	pSBX4C5-2GateSspDnaE(C)			95	0.0	707			A326#2	DD1477	double insert insertion
1538	O326-2	O326		02-10	15:00	Lisa	Growth	1083C2	Qiagen miniprep kit	Plasmid	326	326	pSBX4C5-2GateSspDnaE(C)				0.0	708			A326#2
1539	O326-3	O326		02-10	15:00	Lisa	Growth	1083C3	Qiagen miniprep kit	Plasmid	326	326	pSBX4C5-2GateSspDnaE(C)			91	0.0	709			A326#2	DD1478	tripple insert insertion
1540	O346	O346		02-10	18:00	Lisa	Growth	1090C2	Qiagen miniprep kit	Plasmid	346	346	pSB1C3-EX-BsaI-CBD				0.0	711			G346	DD1479	no.....GG could not work
1541	O346-2	O346		02-10	18:00	Lisa	Growth	1090C4	Qiagen miniprep kit	Plasmid	346	346	pSB1C3-EX-BsaI-CBD				0.0	713			G346	DD1480	no.....GG could not work
1542	O346-3	O346		02-10	18:00	Lisa	Growth	1090C5	Qiagen miniprep kit	Plasmid	346	346	pSB1C3-EX-BsaI-CBD				#DIV/0!	714			G346	28HE43	no.....GG could not work
1543	O364-2	O364		02-10	23:45	MaxW	Growth	KH38.2	Qiagen miniprep kit	Plasmid	364	364	pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)	H2O	30.0	201	25.0	671
1544	O365-2	O365		02-10	23:45	MaxW	Growth	KH39PstI.1	Qiagen miniprep kit	Plasmid	365	365	pSBX4C5-1GateNpuDnaE(N)-H6-1GateNpuDnaE(C)-H6	H2O	30.0	134	30.0	672
1545	O366-2	O366		02-10	23:45	MaxW	Growth	KH39.2	Qiagen miniprep kit	Plasmid	366	366	pSBX4C5-1GateNpuDnaE(N)-H6-H6-1GateNpu(C)_v2	H2O	30.0	372	27.3	673
1546	O260-5	O260		10-03	9:30	Jakob	PCR	1979	PCR purification kit	Plasmid	260	260	pSBX1K3-BBa_I13507-GSGIDECHT	H2O	15.0		15.0
1547	O261-3	O261		10-03	9:30	Jakob	PCR	1980	PCR purification kit	Plasmid	261	261	pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha	H2O	15.0		15.0
1548	R61-4	R61		10-04	22:15	MaxW	Oligos	37,38	Annealing	Restriction	61		T7prom	H2O	50.0	400	49.5
1549	R416	R416		10-04	22:15	MaxW	Gel	426-2	Gel extraction	Restriction	416		pSB1C3-BBa_J04450 (E/S)	H2O	20.0	5	0.1
1550	R417	R417		10-04	22:15	MaxW	Gel	426-3	Gel extraction	Restriction	417		pSBX1K3-BBa_I13507 (E/S)	H2O	20.0	20	14.3
1551	R418	R418		10-04	22:15	MaxW	Gel	426-4	Gel extraction	Restriction	418		pSBX4C5-2Zv2Npu:LaLys+bad1 (E/S)	H2O	20.0	8	12.9
1552	R419	R419		10-04	22:15	MaxW	Gel	426-5	Gel extraction	Restriction	419		pSBX4C5-1GateNpuDnaE(N)*-H6 (E/P)	H2O	20.0	7	11.4
1553	R420	R420		10-04	22:15	MaxW	Gel	426-6	Gel extraction	Restriction	420		pSBX4C5-1GateSspGyrB-S11C_H6 (E/P)	H2O	20.0	8	12.8
1554	R421	R421		10-04	22:15	MaxW	Gel	426-7	Gel extraction	Restriction	421		pSBX4C5-H6-1GateNpu*(C)_v2 (E/P)	H2O	20.0	7	12.1
1555	R422	R422		10-04	23:15	MaxW	Gel	427-2	Gel extraction	Restriction	422		pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C) (E/P)	H2O	20.0	5	9.5
1556	R423	R423		10-04	23:15	MaxW	Gel	427-3	Gel extraction	Restriction	423		pSBX4C5-1GateNpuDnaE(N)-H6-H6-1GateNpu(C)_v2 (E/P)	H2O	20.0	5	9.3
1557	O347	O347		10-04	2:00	MaxW	Growth	1097C1	Qiagen miniprep kit	Plasmid	347	347	pSB1C3-2Zv2Npu	H2O	50.0	284	50.0	717			S347
1558	O348	O348		10-04	2:00	MaxW	Growth	1098C1	Qiagen miniprep kit	Plasmid	348	348	pSB1C3-T7RBS-LambdaLys-IGEMHD_v3	H2O	50.0	163	50.0	718			S348
1559	O349	O349		10-04	2:00	MaxW	Growth	1099C1	Qiagen miniprep kit	Plasmid	349	349	pSB1C3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6	H2O	50.0	222	50.0	719			S349
1560	O350	O350		10-04	2:00	MaxW	Growth	1100C1	Qiagen miniprep kit	Plasmid	350	350	pSB1C3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6	H2O	50.0	210	50.0	720			S350
1561	O351	O351		10-04	2:00	MaxW	Growth	1101C1	Qiagen miniprep kit	Plasmid	351	351	pSB1C3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6	H2O	50.0	231	50.0	721			S351
1562	O352	O352		10-04	2:00	MaxW	Growth	1102C1	Qiagen miniprep kit	Plasmid	352	352	pSB1C3-2Zv2Npu:(LambdaLys-H6)	H2O	50.0	223	50.0	722			S352
1563	O353	O353		10-04	2:00	MaxW	Growth	1103C1	Qiagen miniprep kit	Plasmid	353	353	pSB1C3-2Zv2Npu:(LambdaLys-Rigid)	H2O	50.0	228	50.0	723			S353
1564	O354	O354		10-04	2:00	MaxW	Growth	1104C1	Qiagen miniprep kit	Plasmid	354	354	pSB1C3-2Zv2Npu:Xyla_BsaI_circ_His	H2O	50.0	272	50.0	724			S354
1565	O355	O355		10-04	2:00	MaxW	Growth	1105C2	Qiagen miniprep kit	Plasmid	355	355	pSB1C3-2Zv2Npu:Xyla_circ_noHis	H2O	50.0	296	50.0	725			S355
1566	O356	O356		10-04	2:00	MaxW	Growth	1106C1	Qiagen miniprep kit	Plasmid	356	356	pSB1C3-1GateNpuDnaE(N)	H2O	50.0	346	50.0	726			S356
1567	O357	O357		10-04	2:00	MaxW	Growth	1107C1	Qiagen miniprep kit	Plasmid	357	357	pSB1C3-1GateNpuDnaE(C)	H2O	50.0	285	50.0	727			S357
1568	O358	O358		10-04	2:00	MaxW	Growth	1108C1	Qiagen miniprep kit	Plasmid	358	358	pSB1C3-2Zv2Npu:Xyla(H6)destrCext	H2O	50.0	276	50.0	728			S358
1569	O359	O359		10-04	2:00	MaxW	Growth	1109C1	Qiagen miniprep kit	Plasmid	359	359	pSB1C3-T7RBS-FLAG-Smt3-2Zv2Npu	H2O	50.0	371	50.0	729			S359
1570	O360	O360		10-04	2:00	MaxW	Growth	1110C2	Qiagen miniprep kit	Plasmid	360	360	pSB1C3-Xylanase	H2O	50.0	248	50.0	730			S360
1571	O361	O361		10-04	2:00	MaxW	Growth	1111C1	Qiagen miniprep kit	Plasmid	361	361	pSB1C3-LiLaLy(H6)	H2O	50.0	273	50.0	731			S361
1572	O362	O362		10-04	2:00	MaxW	Growth	1112C1	Qiagen miniprep kit	Plasmid	362	362	pSB1C3-XylaH6BB	H2O	50.0	235	50.0	732			S362
1573	O363	O363		10-04	2:00	MaxW	Growth	1113C1	Qiagen miniprep kit	Plasmid	363	363	pSB1C3-XylaH6extBB	H2O	50.0	193	50.0	733			S363
1574	O271-3	O271		10-04	10:00	Caro	Growth	1036C1	Qiagen miniprep kit	Plasmid	271	271	pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6			110	-9.1	765	was not sequenced, but used for Coex, seems right		A271	DG7164
1575	O271-4	O271		10-04	10:00	Caro	Growth	1036C2	Qiagen miniprep kit	Plasmid	271	271	pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6			115	-8.7	766			A271	DG7165
1576	O271-5	O271		10-04	10:00	Caro	Growth	1036C3	Qiagen miniprep kit	Plasmid	271	271	pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6			100	0.0	767			A271
1577	O271-6	O271		10-04	10:00	Caro	Growth	1036C4	Qiagen miniprep kit	Plasmid	271	271	pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6				0.0				A271
1578	O271-7	O271		10-04	10:00	Caro	Growth	1036C5	Qiagen miniprep kit	Plasmid	271	271	pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6				#DIV/0!				A271
1579	O272-4	O272		10-04	10:00	Caro	Growth	1095C1	Qiagen miniprep kit	Plasmid	272	272	pSBX1K3-H6-DnaXC-mRFPc-H6			168	-6.0	768	was not sequenced, but used for Coex, seems right		A272#3	DG7162
1580	O272-5	O272		10-04	10:00	Caro	Growth	1095C2	Qiagen miniprep kit	Plasmid	272	272	pSBX1K3-H6-DnaXC-mRFPc-H6			189	-5.3	769			A272#3	DG7163
1581	O272-6	O272		10-04	10:00	Caro	Growth	1095C3	Qiagen miniprep kit	Plasmid	272	272	pSBX1K3-H6-DnaXC-mRFPc-H6			122	-8.2	770			A272#3
1582	O272-7	O272		10-04	10:00	Caro	Growth	1095C4	Qiagen miniprep kit	Plasmid	272	272	pSBX1K3-H6-DnaXC-mRFPc-H6				0.0				A272#3
1583	O275-4	O275		10-04	10:00	Caro	Growth	1096C1	Qiagen miniprep kit	Plasmid	275	275	pSBX1K3-SspDnaXc*-sfGFP^C-H6			172	-5.8	771			A275#3	DG7166
1584	O275-5	O275		10-04	10:00	Caro	Growth	1096C2	Qiagen miniprep kit	Plasmid	275	275	pSBX1K3-SspDnaXc*-sfGFP^C-H6			172	-5.8	772			A275#3	DG7167
1585	O275-6	O275		10-04	10:00	Caro	Growth	1096C3	Qiagen miniprep kit	Plasmid	275	275	pSBX1K3-SspDnaXc*-sfGFP^C-H6				0.0				A275#3
1586	O275-7	O275		10-04	10:00	Caro	Growth	1096C4	Qiagen miniprep kit	Plasmid	275	275	pSBX1K3-SspDnaXc*-sfGFP^C-H6			169	-5.9	773			A275#3
1587	O312-4	O312		10-04	10:00	Caro	Growth	1125C1	Qiagen miniprep kit	Plasmid	312	312	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225			235	-4.3	774			G312#2	DG7168
1588	O312-5	O312		10-04	10:00	Caro	Growth	1125C2	Qiagen miniprep kit	Plasmid	312	312	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225			289	-3.5	775			G312#2
1589	O312-6	O312		10-04	10:00	Caro	Growth	1125C3	Qiagen miniprep kit	Plasmid	312	312	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225			272	-3.7	776			G312#2
1590	O312-7	O312		10-04	10:00	Caro	Growth	1125C4	Qiagen miniprep kit	Plasmid	312	312	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225				0.0				G312#2
1591	O312-8	O312		10-04	10:00	Caro	Growth	1125C5	Qiagen miniprep kit	Plasmid	312	312	pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225				0.0				G312#2
1592	O313-4	O313		10-04	10:00	Caro	Growth	1126C1	Qiagen miniprep kit	Plasmid	313	313	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168			291	-3.4	777			G313#2	DG7169	ok, good
1593	O313-5	O313		10-04	10:00	Caro	Growth	1126C2	Qiagen miniprep kit	Plasmid	313	313	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168			281	-3.6	778			G313#2
1594	O313-6	O313		10-04	10:00	Caro	Growth	1126C3	Qiagen miniprep kit	Plasmid	313	313	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168			332	-3.0	779			G313#2
1595	O313-7	O313		10-04	10:00	Caro	Growth	1126C4	Qiagen miniprep kit	Plasmid	313	313	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168				0.0				G313#2
1596	O313-8	O313		10-04	10:00	Caro	Growth	1126C5	Qiagen miniprep kit	Plasmid	313	313	pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168				0.0				G313#2
1597	O314-4	O314		10-04	10:00	Caro	Growth	1127C1	Qiagen miniprep kit	Plasmid	314	314	pSBX4C5-0GateNpuDnaE_C_mRFP169-225			305	-3.3	780			G314#2	DG7170
1598	O314-5	O314		10-04	10:00	Caro	Growth	1127C2	Qiagen miniprep kit	Plasmid	314	314	pSBX4C5-0GateNpuDnaE_C_mRFP169-225			250	-4.0	781			G314#2	DG7171
1599	O314-6	O314		10-04	10:00	Caro	Growth	1127C3	Qiagen miniprep kit	Plasmid	314	314	pSBX4C5-0GateNpuDnaE_C_mRFP169-225			338	-3.0	782			G314#2
1600	O315-4	O315		10-04	10:00	Caro	Growth	1128C1	Qiagen miniprep kit	Plasmid	315	315	pSBX4C5-0GateNpuDnaE_N_mRFP1-168			183	-5.5	783			G315#2	DG7172	ok, good
1601	O315-5	O315		10-04	10:00	Caro	Growth	1128C2	Qiagen miniprep kit	Plasmid	315	315	pSBX4C5-0GateNpuDnaE_N_mRFP1-168			268	-3.7	784			G315#2	DG7173	ok, but probably mutation in promoter, if possible use O315-4
1602	O315-6	O315		10-04	10:00	Caro	Growth	1128C3	Qiagen miniprep kit	Plasmid	315	315	pSBX4C5-0GateNpuDnaE_N_mRFP1-168			200	-5.0	785			G315#2
1603	O315-7	O315		10-04	10:00	Caro	Growth	1128C4	Qiagen miniprep kit	Plasmid	315	315	pSBX4C5-0GateNpuDnaE_N_mRFP1-168				0.0				G315#2
1604	O315-8	O315		10-04	10:00	Caro	Growth	1128C5	Qiagen miniprep kit	Plasmid	315	315	pSBX4C5-0GateNpuDnaE_N_mRFP1-168				0.0				G315#2
1605	O371	O371		10-04	15:00	Lisa	Growth	1135C2	Qiagen miniprep kit	Plasmid	371	371	pSBX4C5-1GateSspDnaB(C)			257	-8.9	734			O240
1606	O371-2	O371		10-04	15:00	Lisa	Growth	1135C3	Qiagen miniprep kit	Plasmid	371	371	pSBX4C5-1GateSspDnaB(C)			99	-15.1	735			O240
1607	O371-3	O371		10-04	15:00	Lisa	Growth	1136C1	Qiagen miniprep kit	Plasmid	371	371	pSBX4C5-1GateSspDnaB(C)			184	-5.0	736			O240-3
1608	O371-4	O371		10-04	15:00	Lisa	Growth	1136C2	Qiagen miniprep kit	Plasmid	371	371	pSBX4C5-1GateSspDnaB(C)			143	0.0	737			O240-3
1609	O372	O372		10-04	15:00	Lisa	Growth	1137C1	Qiagen miniprep kit	Plasmid	372	372	pSBX4C5-1GateSspDnaB(C*)			188	-5.3	738			O241-2
1610	O372-2	O372		10-04	15:00	Lisa	Growth	1137C2	Qiagen miniprep kit	Plasmid	372	372	pSBX4C5-1GateSspDnaB(C*)			198	-5.1	739			O241-2
1611	O372-3	O372		10-04	15:00	Lisa	Growth	1138C1	Qiagen miniprep kit	Plasmid	372	372	pSBX4C5-1GateSspDnaB(C*)			136	-7.4	740			O241-3
1612	O372-4	O372		10-04	15:00	Lisa	Growth	1138C2	Qiagen miniprep kit	Plasmid	372	372	pSBX4C5-1GateSspDnaB(C*)			150	-6.7	741			O241-3
1613	O373	O373		10-04	15:00	Lisa	Growth	1139C2	Qiagen miniprep kit	Plasmid	373	373	pSBX4C5-1GateSspDnaB(N)			175	-5.7	742			O242
1614	O373-2	O373		10-04	15:00	Lisa	Growth	1139C3	Qiagen miniprep kit	Plasmid	373	373	pSBX4C5-1GateSspDnaB(N)			174	-5.8	743			O242
1615	O373-3	O373		10-04	15:00	Lisa	Growth	1140C1	Qiagen miniprep kit	Plasmid	373	373	pSBX4C5-1GateSspDnaB(N)			189	-5.3	744			O242-2
1616	O373-4	O373		10-04	15:00	Lisa	Growth	1140C2	Qiagen miniprep kit	Plasmid	373	373	pSBX4C5-1GateSspDnaB(N)			295	-3.4	745			O242-2
1617	O374	O374		10-04	15:00	Lisa	Growth	1141C2	Qiagen miniprep kit	Plasmid	374	374	pSBX4C5-1GateSspDnaB(N*)			118	-8.5	746			O243-2
1618	O374-2	O374		10-04	15:00	Lisa	Growth	1141C3	Qiagen miniprep kit	Plasmid	374	374	pSBX4C5-1GateSspDnaB(N*)			198	-5.1	747			O243-2
1619	O369	O369		10-04	15:00	Lisa	Growth	1143C1	Qiagen miniprep kit	Plasmid	369	369	pSBX4C5-1GateSspDnaE(N)			207	-4.8	748			O325
1620	O369-2	O369		10-04	15:00	Lisa	Growth	1143C2	Qiagen miniprep kit	Plasmid	369	369	pSBX4C5-1GateSspDnaE(N)			203	-4.9	749			O325
1621	O369-3	O369		10-04	15:00	Lisa	Growth	1144C1	Qiagen miniprep kit	Plasmid	369	369	pSBX4C5-1GateSspDnaE(N)			188	-5.3	750			O325-2
1622	O369-4	O369		10-04	15:00	Lisa	Growth	1144C3	Qiagen miniprep kit	Plasmid	369	369	pSBX4C5-1GateSspDnaE(N)			271	-3.7	751			O325-2
1623	O370	O370		10-04	15:00	Lisa	Growth	1145C2	Qiagen miniprep kit	Plasmid	370	370	pSBX4C5-1GateSspDnaE(C)			224	-4.5	752			O326
1624	O370-2	O370		10-04	15:00	Lisa	Growth	1145C3	Qiagen miniprep kit	Plasmid	370	370	pSBX4C5-1GateSspDnaE(C)			229	-4.4	753			O326
1625	O370-3	O370		10-04	15:00	Lisa	Growth	1146C2	Qiagen miniprep kit	Plasmid	370	370	pSBX4C5-1GateSspDnaE(C)			197	-5.1	754			O326-3
1626	O370-4	O370		10-04	15:00	Lisa	Growth	1146C3	Qiagen miniprep kit	Plasmid	370	370	pSBX4C5-1GateSspDnaE(C)			302	-3.3	755			O326-3
1627	O375	O375		10-04	21:00	MaxW	Growth	1116C1	Qiagen miniprep kit	Plasmid	375	375	pSB1C3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6	H2O	50.0	349	50.0	756			S375
1628	O376	O376		10-04	21:00	MaxW	Growth	1117C1	Qiagen miniprep kit	Plasmid	376	376	pSB1C3-1Gate-NpuDnaE(N)-H6	H2O	50.0	461	37.0	757			S376
1629	O377	O377		10-04	21:00	MaxW	Growth	1118C1	Qiagen miniprep kit	Plasmid	377	377	pSB1C3-H6-1GateNpuDnaE(C)	H2O	50.0	306	50.0	758			S377
1630	O378	O378		10-04	21:00	MaxW	Growth	1119C1	Qiagen miniprep kit	Plasmid	378	378	pSB1C3-T7RBS-H6-sfGFP_T65C-H6	H2O	50.0	366	50.0	759			S378
1631	O379	O379		10-04	21:00	MaxW	Growth	1120C1	Qiagen miniprep kit	Plasmid	379	379	pSB1C3-1GateSspDnaX-S11(C)-H6	H2O	50.0	386	50.0	760			S379
1632	O380	O380		10-04	21:00	MaxW	Growth	1121C1	Qiagen miniprep kit	Plasmid	380	380	pSB1C3-1GateSspDnaX-S11N_H6	H2O	50.0	336	50.0	761			S380
1633	O381	O381		10-04	21:00	MaxW	Growth	1122C1	Qiagen miniprep kit	Plasmid	381	381	pSB1C3-1GateSspGyrB-S11N_H6	H2O	50.0	334	50.0	762			S381
1634	O382	O382		10-04	21:00	MaxW	Growth	1123C1	Qiagen miniprep kit	Plasmid	382	382	pSB1C3-1GateTerDnaE3-S11C_H6	H2O	50.0	273	50.0	763			S382
1635	O383	O383		10-04	21:00	MaxW	Growth	1124C1	Qiagen miniprep kit	Plasmid	383	383	pSB1C3-1GateTerDnaE3-S11(N)-H6	H2O	50.0	304	50.0	764			S383
1636	R470	R470		10-04	22:00	Lisa	Gel	434-2	Gel extraction	Restriction	470		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	15	20.6
1637	R471	R471		10-04	22:00	Lisa	Gel	434-3	Gel extraction	Restriction	471		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	15	20.6
1638	R472	R472		10-04	22:00	Lisa	Gel	434-4	Gel extraction	Restriction	472		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	15	20.6
1639	R473	R473		10-04	22:00	Lisa	Gel	434-5	Gel extraction	Restriction	473		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	15	21.2
1640	R474	R474		10-04	22:00	Lisa	Gel	434-6	Gel extraction	Restriction	474		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	15	21.0
1641	R475	R475		10-04	22:00	Lisa	Gel	434-7	Gel extraction	Restriction	475		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	15	21.0
1642	R476	R476		10-04	22:00	Lisa	Gel	434-8	Gel extraction	Restriction	476		pSB1C3-BBa_J04450 (E/P)	H2O	30.0	15	23.8
1643	R477	R477		10-04	22:00	Lisa	Gel	435-2	Gel extraction	Restriction	477		pSBX4C5-1GateSspDnaB(C*) (E/P)	H2O	30.0	10	20.0
1644	R478	R478		10-04	22:00	Lisa	Gel	435-3	Gel extraction	Restriction	478		pSBX4C5-1GateSspDnaB(C*) (E/P)	H2O	30.0	10	20.0
1645	R479	R479		10-04	22:00	Lisa	Gel	435-4	Gel extraction	Restriction	479		pSBX4C5-1GateSspDnaB(C*) (E/P)	H2O	30.0	10	20.0
1646	R480	R480		10-04	22:00	Lisa	Gel	435-5	Gel extraction	Restriction	480		pSBX4C5-1GateSspDnaB(C*) (E/P)	H2O	30.0	10	20.0
1647	R481	R481		10-04	22:00	Lisa	Gel	435-6	Gel extraction	Restriction	481		pSBX4C5-1GateSspDnaB(N) (E/P)	H2O	30.0	10	20.6
1648	R482	R482		10-04	22:00	Lisa	Gel	435-7	Gel extraction	Restriction	482		pSBX4C5-1GateSspDnaB(N) (E/P)	H2O	30.0	10	20.6
1649	R483	R483		10-04	22:00	Lisa	Gel	435-8	Gel extraction	Restriction	483		pSBX4C5-1GateSspDnaB(N) (E/P)	H2O	30.0	10	20.6
1650	R484	R484		10-04	22:00	Lisa	Gel	436-2	Gel extraction	Restriction	484		pSBX4C5-1GateSspDnaB(N) (E/P)	H2O	30.0	10	20.6
1651	R485	R485		10-04	22:00	Lisa	Gel	436-3	Gel extraction	Restriction	485		pSBX4C5-1GateSspDnaB(N*) (E/P)	H2O	30.0	10	20.6
1652	R486	R486		10-04	22:00	Lisa	Gel	436-4	Gel extraction	Restriction	486		pSBX4C5-1GateSspDnaB(N*) (E/P)	H2O	30.0	10	20.6
1653	R487	R487		10-04	22:00	Lisa	Gel	436-5	Gel extraction	Restriction	487		pSBX4C5-1GateSspDnaE(N) (E/P)	H2O	30.0	10	20.1
1654	R488	R488		10-04	22:00	Lisa	Gel	436-6	Gel extraction	Restriction	488		pSBX4C5-1GateSspDnaE(N) (E/P)	H2O	30.0	10	20.1
1655	R489	R489		10-04	22:00	Lisa	Gel	436-7	Gel extraction	Restriction	489		pSBX4C5-1GateSspDnaE(N) (E/P)	H2O	30.0	10	20.1
1656	R490	R490		10-04	22:00	Lisa	Gel	436-8	Gel extraction	Restriction	490		pSBX4C5-1GateSspDnaE(N) (E/P)	H2O	30.0	10	20.1
1657	R491	R491		10-04	22:00	Lisa	Gel	436-9	Gel extraction	Restriction	491		pSBX4C5-1GateSspDnaE(C) (E/P)	H2O	30.0	10	20.5
1658	R492	R492		10-04	22:00	Lisa	Gel	436-10	Gel extraction	Restriction	492		pSBX4C5-1GateSspDnaE(C) (E/P)	H2O	30.0	10	20.5
1659	R493	R493		10-04	22:00	Lisa	Gel	436-11	Gel extraction	Restriction	493		pSBX4C5-1GateSspDnaE(C) (E/P)	H2O	30.0	10	20.5
1660	R494	R494		10-04	22:00	Lisa	Gel	436-12	Gel extraction	Restriction	494		pSBX4C5-1GateSspDnaE(C) (E/P)	H2O	30.0	10	20.5
1661	R495	R495		10-04	22:00	Lisa	Gel	436-13	Gel extraction	Restriction	495		pSBX4C5-1GateSspDnaB(C) (E/P)	H2O	30.0	10	20.0
1662	R496	R496		10-04	22:00	Lisa	Gel	436-14	Gel extraction	Restriction	496		pSBX4C5-1GateSspDnaB(C) (E/P)	H2O	30.0	10	20.0
1663	R424	R424		10-04	22:00	Caro	Restriction	424	Gel extraction	Restriction	424		pSBX4C5-0GateSspDnaX-S11C_mRFP169-225 (E/X)			18	-4.0
1664	R425	R425		10-04	22:00	Caro	Restriction	425	Gel extraction	Restriction	425		pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 (E/X)			30	-2.1
1665	R426	R426		10-04	22:00	Caro	Restriction	426	Gel extraction	Restriction	426		pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 (E/X)			16	-4.6
1666	R427	R427		10-04	22:00	Caro	Gel	437-2	Gel extraction	Restriction	427		pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 (E/S)			8	-7.8
1667	R428	R428		10-04	22:00	Caro	Gel	437-3	Gel extraction	Restriction	428		pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 (E/S)			7	-8.7
1668	R429	R429		10-04	22:00	Caro	Gel	437-4	Gel extraction	Restriction	429		pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)			8	-8.1
1669	R430	R430		10-04	22:00	Caro	Restriction	430	PCR purification kit	Restriction	430		pSBX4C5-0GateSspDnaX-S11C_mRFP169-225 (E/X)			17	-3.1
1670	R431	R431		10-04	22:00	Caro	Restriction	431	PCR purification kit	Restriction	431		pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 (E/X)			21	-4.8
1671	R432	R432		10-04	22:00	Caro	Restriction	432	PCR purification kit	Restriction	432		pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 (E/X)			20	-2.4
1672	R433	R433		10-04	22:00	Caro	Restriction	433	PCR purification kit	Restriction	433		pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 (E/X)			30	-1.7
1673	R434	R434		10-04	22:00	Caro	Restriction	434	PCR purification kit	Restriction	434		pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 (E/X)			28	-2.6
1674	R435	R435		10-04	22:00	Caro	Restriction	435	PCR purification kit	Restriction	435		pSBX4C5-0GateNpuDnaE_C_mRFP169-225 (E/X)			23	-3.3
1675	R436	R436		10-04	22:00	Caro	Restriction	436	PCR purification kit	Restriction	436		pSBX4C5-0GateNpuDnaE_C_mRFP169-225 (E/X)			26	-3.0
1676	R437	R437		10-04	22:00	Caro	Restriction	437	PCR purification kit	Restriction	437		pSBX4C5-0GateNpuDnaE_C_mRFP169-225 (E/X)			30	-2.7
1677	R438	R438		10-04	22:00	Caro	Gel	437-5	Gel extraction	Restriction	438		pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 (E/S)			6	-7.7
1678	R439	R439		10-04	22:00	Caro	Gel	437-6	Gel extraction	Restriction	439		pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 (E/S)			5	-18.7
1679	R440	R440		10-04	22:00	Caro	Gel	437-7	Gel extraction	Restriction	440		pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)			5	-8.4
1680	R441	R441		10-04	22:00	Caro	Gel	437-8	Gel extraction	Restriction	441		pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)			5	-9.0
1681	R442	R442		10-04	22:00	Caro	Gel	438-2	Gel extraction	Restriction	442		pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 (E/S)			8	-8.1
1682	R443	R443		10-04	22:00	Caro	Gel	438-3	Gel extraction	Restriction	443		pSBX4C5-0GateNpuDnaE_N_mRFP1-168 (E/S)			8	-7.4
1683	R444	R444		10-04	22:00	Caro	Gel	438-4	Gel extraction	Restriction	444		pSBX4C5-0GateNpuDnaE_N_mRFP1-168 (E/S)			8	-7.7
1684	R445	R445		10-04	22:00	Caro	Gel	438-5	Gel extraction	Restriction	445		pSBX4C5-0GateNpuDnaE_N_mRFP1-168 (E/S)			8	-8.0
1685	R446	R446		10-04	22:00	Caro	Gel	438-6	Gel extraction	Restriction	446		pSBX1K3-H6-mRFPn-DnaXN-H6 (E/S)			6	-28.4
1686	R447	R447		10-04	22:00	Caro	Gel	438-7	Gel extraction	Restriction	447		pSBX1K3-H6-mRFPn-DnaXN-H6 (E/S)			6	-42.4
1687	R448	R448		10-04	22:00	Caro	Gel	438-8	Gel extraction	Restriction	448		pSBX1K3-H6-mRFPn-DnaXN-H6 (E/S)			6	-13.6
1688	R449	R449		10-04	22:00	Caro	Gel	439-2	Gel extraction	Restriction	449		pSBX1K3-H6-mRFPn-DnaXN*-H6 (E/S)			4	-7.7
1689	R450	R450		10-04	22:00	Caro	Restriction	450	PCR purification kit	Restriction	450		pSBX1K3-H6-DnaXC-mRFPc-H6 (E/X)			15	-3.2
1690	R451	R451		10-04	22:00	Caro	Restriction	451	PCR purification kit	Restriction	451		pSBX1K3-H6-DnaXC-mRFPc-H6 (E/X)			15	-3.2
1691	R452	R452		10-04	22:00	Caro	Restriction	452	PCR purification kit	Restriction	452		pSBX1K3-H6-DnaXC-mRFPc-H6 (E/X)			13	-3.5
1692	R453	R453		10-04	22:00	Caro	Restriction	453	PCR purification kit	Restriction	453		pSBX1K3-H6-DnaXC*-mRFPc-H6 (E/X)			5	-5.1
1693	R454	R454		10-04	22:00	Caro	Restriction	454	PCR purification kit	Restriction	454		pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 (E/X)			13	-3.3
1694	R455	R455		10-04	22:00	Caro	Restriction	455	PCR purification kit	Restriction	455		pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 (E/X)			17	-6.0
1695	R456	R456		10-04	22:00	Caro	Restriction	456	PCR purification kit	Restriction	456		pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 (E/X)			15	-3.0
1696	R457	R457		10-04	22:00	Caro	Restriction	457	PCR purification kit	Restriction	457		pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 (E/X)			10	-4.0
1697	R458	R458		10-04	22:00	Caro	Restriction	458	PCR purification kit	Restriction	458		pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 (E/X)			10	-4.0
1698	R459	R459		10-04	22:00	Caro	Gel	439-3	Gel extraction	Restriction	459		pSBX1K3-DnaXN-sfGFPn-H6 (E/S)			8	-23.3
1699	R460	R460		10-04	22:00	Caro	Gel	439-4	Gel extraction	Restriction	460		pSBX1K3-DnaXN-sfGFPn-H6 (E/S)			8	-6.8
1700	R461	R461		10-04	22:00	Caro	Gel	439-5	Gel extraction	Restriction	461		pSBX1K3-DnaXN-sfGFPn-H6 (E/S)			8	-7.2
1701	R462	R462		10-04	22:00	Caro	Gel	439-6	Gel extraction	Restriction	462		pSBX1K3-DnaXN*-sfGFPn-H6 (E/S)			8	-20.8
1702	R463	R463		10-04	22:00	Caro	Restriction	463	PCR purification kit	Restriction	463		pSBX1K3-SspDnaXc-sfGFP^C-H6 (E/X)			22	-3.5
1703	R464	R464		10-04	22:00	Caro	Restriction	464	PCR purification kit	Restriction	464		pSBX1K3-SspDnaXc*-sfGFP^C-H6 (E/X)			19	-3.9
1704	R465	R465		10-04	22:00	Caro	Restriction	465	PCR purification kit	Restriction	465		pSBX1K3-SspDnaXc*-sfGFP^C-H6 (E/X)			19	-3.9
1705	R466	R466		10-04	22:00	Caro	Restriction	466	PCR purification kit	Restriction	466		pSBX1K3-SspDnaXc*-sfGFP^C-H6 (E/X)			20	-3.7
1706	R467	R467		10-04	22:00	Caro	Restriction	467	PCR purification kit	Restriction	467		pSBX1K3-LOV-BsaIIasB-sfGFPc (E/X)			28	-3.3
1707	R468	R468		10-04	22:00	Caro	Restriction	468	PCR purification kit	Restriction	468		pSBX1K3-LOV-12-DnaXC-sfGFPc (E/X)			21	-4.0
1708	R469	R469		10-04	22:00	Caro	Restriction	469	PCR purification kit	Restriction	469		pSBX1K3-LOV-6-DnaXC-sfGFPc (E/X)			25	-7.8
1709	R497	R497		10-05	17:30	MaxW	Gel	440-2	Gel extraction	Restriction	497		pSBX4C5-1GateGp41-1(N)-H6 (E/P)	H2O	20.0	14	12.7
1710	R498	R498		10-05	17:30	MaxW	Gel	440-3	Gel extraction	Restriction	498		pSBX4C5-1GateGp41-1(N)-H6 (E/P)	H2O	20.0	11	12.4
1711	R499	R499		10-05	17:30	MaxW	Gel	440-4	Gel extraction	Restriction	499		pSBX4C5-H6-1GateGp41-1(C) (E/P)	H2O	20.0	6	8.5
1712	R500	R500		10-05	17:30	MaxW	Gel	440-5	Gel extraction	Restriction	500		pSBX4C5-H6-1GateGp41-1(C) (E/P)	H2O	20.0	6	8.5
1713	R501	R501		10-05	17:30	MaxW	Gel	440-6	Gel extraction	Restriction	501		pSBX4C5-DoubleGateGp41-1(N) (E/P)	H2O	20.0	9	8.2
1714	R502	R502		10-05	17:30	MaxW	Gel	440-7	Gel extraction	Restriction	502		pSBX4C5-DoubleGateGp41-1(N) (E/P)	H2O	20.0	7	8.1
1715	R503	R503		10-05	17:30	MaxW	Gel	440-8	Gel extraction	Restriction	503		pSBX4C5-DoubleGateGp41-1(C) (E/P)	H2O	20.0	11	12.1
1716	R504	R504		10-05	17:30	MaxW	Gel	440-9	Gel extraction	Restriction	504		pSBX4C5-DoubleGateGp41-1(C) (E/P)	H2O	20.0	11	12.1
1717	R505	R505		10-05	17:30	MaxW	Gel	440-10	Gel extraction	Restriction	505		pSBX4C5-DoubleNpuDnaE(N) (E/P)	H2O	20.0	9	8.4
1718	R506	R506		10-05	17:30	MaxW	Gel	440-11	Gel extraction	Restriction	506		pSBX4C5-DoubleNpuDnaE(N) (E/P)	H2O	20.0	7	8.2
1719	R507	R507		10-05	17:30	MaxW	Gel	440-12	Gel extraction	Restriction	507		pSBX4C5-DoubleGateNpuDnaE(C) (E/P)	H2O	20.0	13	12.4
1720	R509	R509		10-05	17:30	MaxW	Gel	441-2, 441-3	Gel extraction	Restriction	509		pSB1C3-1Gate-NpuDnaE(N)-H6 (E/P)	H2O	20.0	32	15.0
1721	R511	R511		10-05	17:30	MaxW	Gel	441-4, 441-5	Gel extraction	Restriction	511		pSB1C3-1Gate-NpuDnaE(N)-H6 (E/P)	H2O	20.0	28	9.2
1722	R513	R513		10-05	17:30	MaxW	Gel	441-6, 441-7	Gel extraction	Restriction	513		pSB1C3-1Gate-NpuDnaE(N)-H6 (E/P)	H2O	20.0	24	16.8
1723	R515	R515		10-05	17:30	MaxW	Gel	441-8, 441-9	Gel extraction	Restriction	515		pSB4C5-BBa_J04450 (E/P)	H2O	20.0	16	9.7
1724	R517	R517		10-05	17:30	MaxW	Gel	441-10, 441-11	Gel extraction	Restriction	517		pSB4C5-BBa_J04450 (E/P)	H2O	20.0	16	9.3
1725	R519	R519		10-05	17:30	MaxW	Gel	441-12, 441-13, 442-2	Gel extraction	Restriction	519		pSB4C5-BBa_J04450 (E/P)	H2O	20.0	19	7.7
1726	R522	R522		10-05	17:30	MaxW	Gel	442-3	Gel extraction	Restriction	522		pSBX1A3-BBa_I13507 (E/P)	H2O	20.0	14	16.3
1727	R523	R523		10-05	17:30	MaxW	Gel	442-4, 442-5	Gel extraction	Restriction	523		pSBX1C3-BBa_I13507 (E/P)	H2O	20.0	7	12.5
1728	R524	R524		10-05	17:30	MaxW	Gel	442-6	Gel extraction	Restriction	524		pSBX1K3-BBa_I13507 (E/P)	H2O	20.0	27	17.9
1729	R525	R525		10-05	17:30	MaxW	Gel	442-7	Gel extraction	Restriction	525		pSBX1T3-T7RBS-IGEMHD_v3 (E/P)	H2O	20.0	16	16.3
1730	R526	R526		10-05	17:30	MaxW	Gel	442-8, 442-9	Gel extraction	Restriction	526		pSBX4A5-BBa_I13507 (E/P)	H2O	20.0	16	15.8
1731	R527	R527		10-05	17:30	MaxW	Gel	442-10	Gel extraction	Restriction	527		pSBX4C5-BBa_I13507 (E/P)	H2O	20.0	14	15.0
1732	R528	R528		10-05	17:30	MaxW	Gel	442-11	Gel extraction	Restriction	528		pSBX4K5-BBa_I13507 (E/P)	H2O	20.0	3	9.0
1733	O398	O398		10-05	19:54	Jan	Growth	1149C1	Qiagen miniprep kit	Plasmid	398	398	pSB1C3-1GateNpuDnaE(N)*-H6	EB	50.0	326	50.0	786			S398
1734	O399	O399		10-05	19:54	Jan	Growth	1150C1	Qiagen miniprep kit	Plasmid	399	399	pSB1C3-1GateSspGyrB-S11C_H6	EB	50.0	388	50.0	787			S399
1735	O400	O400		10-05	19:54	Jan	Growth	1151C1	Qiagen miniprep kit	Plasmid	400	400	pSB1C3-H6-1GateNpu*(C)_v2	EB	50.0	264	50.0	788			S400
1736	O401	O401		10-05	19:54	Jan	Growth	1152C1	Qiagen miniprep kit	Plasmid	401	401	pSB1C3-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)	EB	50.0	0	50.0	789	accidentally used QIAshredder column		S401
1737	O402	O402		10-05	19:54	Jan	Growth	1153C1	Qiagen miniprep kit	Plasmid	402	402	pSB1C3-1GateNpuDnaE(N)-H6-H6-1GateNpu(C)_v2	EB	50.0	0	50.0	790	accidentally used QIAshredder column		S402
1738	O403	O403		10-05	19:54	Jan	Growth	1154C1	Qiagen miniprep kit	Plasmid	403	403	pSB1C3-T7RBS_BBv2	EB	50.0	0	50.0	791	accidentally used QIAshredder column		S403
1739	O403-2	O403		10-05	19:54	Jan	Growth	1154C2	Qiagen miniprep kit	Plasmid	403	403	pSB1C3-T7RBS_BBv2	EB	50.0	0	50.0	792	accidentally used QIAshredder column		S403
1740	O284-3	O284		10-05	19:54	Jan	Growth	1155C1	Qiagen miniprep kit	Plasmid	284	284	pSBX1K3-2Zv2Npu:LaLys+bad1	EB	50.0	0	50.0	793	accidentally used QIAshredder column		S284
1741	O63-7	O63		10-05	23:00	Anna	Growth	240C2-2	Qiagen miniprep kit	Plasmid	63	63	pSBX4K5-BBa_I13507	H2O	30.0	119	21.6				L63#3
1742	R530	R530		10-06	11:10	Silvan	Gel	443-2	Gel extraction	Restriction	530		pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) (E/P)	EB	25.0	3	13.3
1743	R531	R531		10-06	11:10	Silvan	Gel	443-3	Gel extraction	Restriction	531		pSBX1K3-T7RBS-H6-Smt3-0ZSortA (E/P)	EB	25.0	8	8.9
1744	R532	R532		10-06	11:10	Silvan	Gel	443-4	Gel extraction	Restriction	532		pSBX1K3-T7RBS-H6-0ZSortA (E/P)	EB	25.0	8	9.0
1745	P234-4	P234		10-06	11:10	Silvan	Gel	444-2,444-3	Gel extraction	PP	234		pSB1C3_SortAc-H6_TEVc-BsaI	EB	25.0	21	25.0
1746	P521	P521		10-06	11:10	Silvan	Gel	444-4,444-5	Gel extraction	PP	521		pSB1C3_TAATAA_Smt3	EB	25.0	82	25.0
1747	P522	P522		10-06	11:10	Silvan	Gel	444-6,444-7	Gel extraction	PP	522		H6Smt3_BBprefix_DNMT1/BB2	EB	25.0	70	25.0
1748	P235-3	P235		10-06	11:10	Silvan	Gel	444-10,444-11	Gel extraction	PP	235		mRFPexp_TEVc-BsaI_SortAc-H6-BBsuffix	EB	25.0	137	25.0
1749	P523	P523		10-06	11:10	Silvan	Gel	444-12,444-13	Gel extraction	PP	523		DNMT1/BB2_DNMT1/BB1_DNMT1/BB3	EB	25.0	29	25.0
1750	P524	P524		10-06	11:10	Silvan	Gel	444-14,444-15	Gel extraction	PP	524		DNMT1/BB3_DNMT1/BB2_RFC10suffix	EB	25.0	34	25.0
1751	R529	R529		10-06	13:00	Anna	Gel	445-2,445-3	Gel extraction	Restriction	529		pSBX4K5-BBa_I13507 (E/P)	H2O	20.0	10	13.4
1752	O414	O414		10-06	16:50	Lisa	Growth	1156C2	Qiagen miniprep kit	Plasmid	414	414	pSB1C3-1GateSspDnaB(C*)	H2O	50.0	241	45.0	811			S414	DG7142, IC5216, IC5221	bad, SspDnaEN, SspDnaEN
1753	O414-2	O414		10-06	16:50	Lisa	Growth	1157C1	Qiagen miniprep kit	Plasmid	414	414	pSB1C3-1GateSspDnaB(C*)	H2O	50.0	226	45.0	812			S414#2	DG7143
1754	O414-3	O414		10-06	16:50	Lisa	Growth	1158C1	Qiagen miniprep kit	Plasmid	414	414	pSB1C3-1GateSspDnaB(C*)	H2O	50.0	161	45.0	813			S414#3	DG7144
1755	O414-4	O414		10-06	16:50	Lisa	Growth	1159C2	Qiagen miniprep kit	Plasmid	414	414	pSB1C3-1GateSspDnaB(C*)	H2O	50.0	193	45.0	814			S414#4	DG7145
1756	O415	O415		10-06	16:50	Lisa	Growth	1160C2	Qiagen miniprep kit	Plasmid	415	415	pSB1C3-1GateSspDnaB(N)	H2O	50.0	144	45.0	815			S415	DG7146, IC5213, IC5218	bad, actually SspDnaEC, actually SspDnaEC
1757	O415-2	O415		10-06	16:50	Lisa	Growth	1161C1	Qiagen miniprep kit	Plasmid	415	415	pSB1C3-1GateSspDnaB(N)	H2O	50.0	111	45.0	816			S415#2	DG7147	bad
1758	O415-3	O415		10-06	16:50	Lisa	Growth	1162C1	Qiagen miniprep kit	Plasmid	415	415	pSB1C3-1GateSspDnaB(N)	H2O	50.0	315	45.0	817			S415#3	DG7148	bad
1759	O415-4	O415		10-06	16:50	Lisa	Growth	1163C2	Qiagen miniprep kit	Plasmid	415	415	pSB1C3-1GateSspDnaB(N)	H2O	50.0	213	45.0	818			S415#4	DG7149	bad
1760	O416	O416		10-06	16:50	Lisa	Growth	1164C1	Qiagen miniprep kit	Plasmid	416	416	pSB1C3-1GateSspDnaB(N*)	H2O	50.0	185	45.0	819			S416	DG7150, IC5215, IC5220	bad, actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie, actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie
1761	O416-2	O416		10-06	16:50	Lisa	Growth	1165C2	Qiagen miniprep kit	Plasmid	416	416	pSB1C3-1GateSspDnaB(N*)	H2O	50.0	73	45.0	824			S416#2	DG7151	not mixed up, missing?
1762	O411	O411		10-06	16:50	Lisa	Growth	1166C2	Qiagen miniprep kit	Plasmid	411	411	pSB1C3-1GateSspDnaE(N)	H2O	50.0	144	45.0	820			S411	DG7152
1763	O411-2	O411		10-06	16:50	Lisa	Growth	1167C1	Qiagen miniprep kit	Plasmid	411	411	pSB1C3-1GateSspDnaE(N)	H2O	50.0	185	45.0	821			S411#2	DG7153
1764	O411-3	O411		10-06	16:50	Lisa	Growth	1168C1	Qiagen miniprep kit	Plasmid	411	411	pSB1C3-1GateSspDnaE(N)	H2O	50.0	226	45.0	822			S411#3	DG7154
1765	O411-4	O411		10-06	16:50	Lisa	Growth	1169C1	Qiagen miniprep kit	Plasmid	411	411	pSB1C3-1GateSspDnaE(N)	H2O	50.0	166	45.0	823			S411#4	DG7155, IC5217, IC5222	, actually SspDnaBC, actually SspDnaBC
1766	O412	O412		10-06	16:50	Lisa	Growth	1170C2	Qiagen miniprep kit	Plasmid	412	412	pSB1C3-1GateSspDnaE(C)	H2O	50.0	113	45.0	825	bad parent		S412	DG7156
1767	O412-2	O412		10-06	16:50	Lisa	Growth	1171C1	Qiagen miniprep kit	Plasmid	412	412	pSB1C3-1GateSspDnaE(C)	H2O	50.0	337	45.0	826	bad parent		S412#2	DG7157, IC5260	, actually sspdnaBN
1768	O412-3	O412		10-06	16:50	Lisa	Growth	1172C1	Qiagen miniprep kit	Plasmid	412	412	pSB1C3-1GateSspDnaE(C)	H2O	50.0	80	45.0	827	bad parent		S412#3	DG7158, IC5261	, actually sspdnaBN
1769	O412-4	O412		10-06	16:50	Lisa	Growth	1173C2	Qiagen miniprep kit	Plasmid	412	412	pSB1C3-1GateSspDnaE(C)	H2O	50.0	139	45.0	828	bad parent		S412#4	DG7159
1770	O413	O413		10-06	16:50	Lisa	Growth	1175C1	Qiagen miniprep kit	Plasmid	413	413	pSB1C3-1GateSspDnaB(C)	H2O	50.0	90	45.0	829			S413#2	DG7160
1771	O413-2	O413		10-06	16:50	Lisa	Growth	1175C2	Qiagen miniprep kit	Plasmid	413	413	pSB1C3-1GateSspDnaB(C)	H2O	50.0	58	45.0	830			S413#2	DG7161, IC5214, IC5219	bad, actually SspDnaBN, actually SspDnaBN
1772	O401-2	O401		10-07	1:20	MaxW	Growth	1152C1-1	Qiagen miniprep kit	Plasmid	401	401	pSB1C3-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)	H2O	50.0	527	50.0				S401
1773	O402-2	O402		10-07	1:20	MaxW	Growth	1153C1-1	Qiagen miniprep kit	Plasmid	402	402	pSB1C3-1GateNpuDnaE(N)-H6-H6-1GateNpu(C)_v2	H2O	50.0	511	50.0				S402
1774	O403-3	O403		10-07	1:20	MaxW	Growth	1154C1-1	Qiagen miniprep kit	Plasmid	403	403	pSB1C3-T7RBS_BBv2	H2O	50.0	345	50.0				S403	IC5223	is actually T7prom
1775	O403-4	O403		10-07	1:20	MaxW	Growth	1154C2-1	Qiagen miniprep kit	Plasmid	403	403	pSB1C3-T7RBS_BBv2	H2O	50.0	285	50.0				S403	IC5224	is actually T7prom
1776	O284-4	O284		10-07	1:20	MaxW	Growth	1155C1-1	Qiagen miniprep kit	Plasmid	284	284	pSBX1K3-2Zv2Npu:LaLys+bad1	H2O	50.0	373	50.0				S284
1777	O417	O417		10-07	1:20	MaxW	Growth	1179C1	Qiagen miniprep kit	Plasmid	417	417	pSB1C3-1GateGp41-1(N)-H6	H2O	50.0	342	50.0	794			S417
1778	O418	O418		10-07	1:20	MaxW	Growth	1181C1	Qiagen miniprep kit	Plasmid	418	418	pSB1C3-H6-1GateGp41-1(C)	H2O	50.0	324	50.0	795			S418
1779	O419	O419		10-07	1:20	MaxW	Growth	1183C1	Qiagen miniprep kit	Plasmid	419	419	pSB1C3-DoubleGateGp41-1(N)	H2O	50.0	416	50.0	796			S419
1780	O420	O420		10-07	1:20	MaxW	Growth	1185C1	Qiagen miniprep kit	Plasmid	420	420	pSB1C3-DoubleGateGp41-1(C)	H2O	50.0	405	50.0	797			S420
1781	O421	O421		10-07	1:20	MaxW	Growth	1187C1	Qiagen miniprep kit	Plasmid	421	421	pSB1C3-DoubleNpuDnaE(N)	H2O	50.0	492	50.0	798			S421
1782	O422	O422		10-07	1:20	MaxW	Growth	1189C1	Qiagen miniprep kit	Plasmid	422	422	pSB1C3-DoubleGateNpuDnaE(C)	H2O	50.0	386	50.0	799			S422
1783	O404	O404		10-07	1:20	MaxW	Growth	1190C1	Qiagen miniprep kit	Plasmid	404	404	pSBX1A3-BBa_J04450	H2O	50.0	428	50.0	800			S404
1784	O405	O405		10-07	1:20	MaxW	Growth	1191C1	Qiagen miniprep kit	Plasmid	405	405	pSBX1C3-BBa_J04450	H2O	50.0	250	50.0	801			S405
1785	O406	O406		10-07	1:20	MaxW	Growth	1192C1	Qiagen miniprep kit	Plasmid	406	406	pSBX1K3-BBa_J04450	H2O	50.0	470	50.0	802			S406
1786	O408	O408		10-07	1:20	MaxW	Growth	1194C1	Qiagen miniprep kit	Plasmid	408	408	pSBX4A5-BBa_J04450	H2O	50.0	258	50.0	803			S408
1787	O409	O409		10-07	1:20	MaxW	Growth	1195C1	Qiagen miniprep kit	Plasmid	409	409	pSBX4C5-BBa_J04450	H2O	50.0	524	50.0	804			S409
1788	R532-2	R532	?	10-07	13:40	Lisa	Restriction	532		Restriction	532		pSBX1K3-T7RBS-H6-0ZSortA (E/P)				0.0
1789	R532-3	R532	?	10-07	13:40	Lisa	Restriction	532		Restriction	532		pSBX1K3-T7RBS-H6-0ZSortA (E/P)				0.0
1790	R532-4	R532	?	10-07	13:40	Lisa	Restriction	532		Restriction	532		pSBX1K3-T7RBS-H6-0ZSortA (E/P)				0.0
1791	R532-5	R532	?	10-07	13:40	Lisa	Restriction	532		Restriction	532		pSBX1K3-T7RBS-H6-0ZSortA (E/P)				0.0
1792	R532-6	R532	?	10-07	13:40	Lisa	Restriction	532		Restriction	532		pSBX1K3-T7RBS-H6-0ZSortA (E/P)				0.0
1793	O386	O386		10-07	16:00	Caro	Growth	1205C1	Qiagen miniprep kit	Plasmid	386	386	pSBX4C5-0GateTer*-coex_mRFP168/169			300	0.0	831			S386#3	IC5185, IC5186	good, ok, good, ok
1794	O387	O387		10-07	16:00	Caro	Growth	1207C1	Qiagen miniprep kit	Plasmid	387	387	pSBX4C5-0GateNpu*-coex_mRFP168/169			320	0.0	832			S387#2	IC5187, IC5188	good, ok, good, ok
1795	O387-2	O387		10-07	16:00	Caro	Growth	1208C1	Qiagen miniprep kit	Plasmid	387	387	pSBX4C5-0GateNpu*-coex_mRFP168/169				0.0	833			S387#3
1796	O384	O384		10-07	16:00	Caro	Growth	1201C2	Qiagen miniprep kit	Plasmid	384	384	pSBX4C5-0GateDnaX*-coex_mRFP168/169			550	0.0	834			S384	IC5189, IC5190	no DnaXN, no DNnaXN
1797	O386-2	O386		10-07	16:00	Caro	Growth	1203C1	Qiagen miniprep kit	Plasmid	386	386	pSBX4C5-0GateTer*-coex_mRFP168/169				0.0	835			S386
1798	O386-3	O386		10-07	16:00	Caro	Growth	1204C2	Qiagen miniprep kit	Plasmid	386	386	pSBX4C5-0GateTer*-coex_mRFP168/169				0.0	836			S386#2
1799	O397	O397		10-07	16:00	Caro	Growth	1209C1	Qiagen miniprep kit	Plasmid	397	397	pSBX1K3-DnaX-mRFP168/169_coex			230	0.0	837			S397	IC5191, IC5192	good, ok, good, ok
1800	O255-3	O255		10-07	16:00	Caro	Growth	1198C1	Qiagen miniprep kit	Plasmid	255	255	pSBX4C5-0GateSspDnaX-coex_mRFP168/169			340	0.0	838			S255#3	IC5193, IC5194	good, ok, good, ok
1801	O255-4	O255		10-07	16:00	Caro	Growth	1198C3	Qiagen miniprep kit	Plasmid	255	255	pSBX4C5-0GateSspDnaX-coex_mRFP168/169				0.0	839			S255#3
1802	O256	O256		10-07	16:00	Caro	Growth	1199C1	Qiagen miniprep kit	Plasmid	256	256	pSBX4C5-0GateSspGyrB-coex_mRFP168/169			360	0.0	840			S256#2	IC5195, IC5196	good, ok, good, ok
1803	O257-3	O257		10-07	16:00	Caro	Growth	1200C1	Qiagen miniprep kit	Plasmid	257	257	pSBX4C5-0GateTerDnaE3-coex_mRFP168/169			550	0.0	841			S257#3	IC5197, IC5198	mutations after Biobrick, good ok, mutations after Biobrick, good ok
1804	O393	O393		10-07	16:00	Caro	Growth	1213C2	Qiagen miniprep kit	Plasmid	393	393	pSBX1K3-H6-LOV-BsaIIasB-mRFP_coex			260	0.0	842			S393	IC5199, IC5200	ok, good, ok, good
1805	O396	O396		10-07	16:00	Caro	Growth	1212C1	Qiagen miniprep kit	Plasmid	396	396	pSBX1K3-DnaX-mRFP*168/169_coex			250	0.0	843			S396	IC5201, IC5202	ok, good, ok, good
1806	O396-2	O396		10-07	16:00	Caro	Growth	1212C2	Qiagen miniprep kit	Plasmid	396	396	pSBX1K3-DnaX-mRFP*168/169_coex				0.0	844			S396
1807	O397-2	O397		10-07	16:00	Caro	Growth	1211C1	Qiagen miniprep kit	Plasmid	397	397	pSBX1K3-DnaX-mRFP168/169_coex				0.0	845			S397#3
1808	O397-3	O397		10-07	16:00	Caro	Growth	1210C2	Qiagen miniprep kit	Plasmid	397	397	pSBX1K3-DnaX-mRFP168/169_coex				0.0	846			S397#2
1809	O395	O395		10-07	16:00	Caro	Growth	1215C1	Qiagen miniprep kit	Plasmid	395	395	pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex			300	0.0	847			S395	IC5203, IC5204	ok, good, probably frameshift after CDS, ok, good, probably frameshift after CDS
1810	O395-2	O395		10-07	16:00	Caro	Growth	1216C1	Qiagen miniprep kit	Plasmid	395	395	pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex				0.0	848			S395#2
1811	O395-3	O395		10-07	16:00	Caro	Growth	1217C1	Qiagen miniprep kit	Plasmid	395	395	pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex				0.0	849			S395#3
1812	O391	O391		10-07	16:00	Caro	Growth	1218C1	Qiagen miniprep kit	Plasmid	391	391	pSBX1K3-DnaX-sfGFP_coex			330	0.0	850			S391	IC5205, IC5206	ok, good, ok,good
1813	O392	O392		10-07	16:00	Caro	Growth	1219C1	Qiagen miniprep kit	Plasmid	392	392	pSBX1K3-DnaX*-sfGFP_coex			210	0.0	851			S392	IC5207, IC5208	ok, good, ok,good
1814	O392-2	O392		10-07	16:00	Caro	Growth	1220C3	Qiagen miniprep kit	Plasmid	392	392	pSBX1K3-DnaX*-sfGFP_coex				0.0	852			S392#2
1815	O392-3	O392		10-07	16:00	Caro	Growth	1221C1	Qiagen miniprep kit	Plasmid	392	392	pSBX1K3-DnaX*-sfGFP_coex				0.0	853			S392#3
1816	O388	O388		10-07	16:00	Caro	Growth	1222C1	Qiagen miniprep kit	Plasmid	388	388	pSBX1K3-LOV-BsaIIasB-sfGFP_coex			190	0.0	854			S388	IC5209, IC5210	ok, good, ok,good
1817	O389	O389		10-07	16:00	Caro	Growth	1223C1	Qiagen miniprep kit	Plasmid	389	389	pSBX1K3-LOV-12-DnaXC-sfGFP_coex			270	0.0	855			S389	IC5211, IC5212	ok, good, ok,good
1818	O235	O235		10-07	18:30	Silvan	Growth	1226C2	Qiagen miniprep kit	Plasmid	235	235	pSB1C3-Smt3-2ZSortA-H6 (mRFP)			166	0.0	858			G235	IC5225, IC5231	C->A point mutation in mRFP barcode, mixture of mRFP and ccdB
1819	O235-2	O235		10-07	18:30	Silvan	Growth	1226C3	Qiagen miniprep kit	Plasmid	235	235	pSB1C3-Smt3-2ZSortA-H6 (mRFP)			198	0.0	859			G235	IC5226,	C->A point mutation in mRFP barcode,
1820	O234	O234		10-07	18:30	Silvan	Growth	1228C2	Qiagen miniprep kit	Plasmid	234	234	pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP)			227	0.0	861			S234
1821	O234-2	O234		10-07	18:30	Silvan	Growth	1228C3	Qiagen miniprep kit	Plasmid	234	234	pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP)			277	0.0	862			S234
1822	O423	O423		10-07	18:30	Silvan	Growth	1229C1	Qiagen miniprep kit	Plasmid	423	423	pSB1C3-T7RBS-H6-Smt3-0ZSortA			206	0.0	863			S423
1823	O423-2	O423		10-07	18:30	Silvan	Growth	1229C2	Qiagen miniprep kit	Plasmid	423	423	pSB1C3-T7RBS-H6-Smt3-0ZSortA			264	0.0	864			S423
1824	O423-3	O423		10-07	18:30	Silvan	Growth	1229C3	Qiagen miniprep kit	Plasmid	423	423	pSB1C3-T7RBS-H6-Smt3-0ZSortA			297	0.0	865			S423
1825	O425	O425		10-07	18:30	Silvan	Growth	1230C1	Qiagen miniprep kit	Plasmid	425	425	pSB1C3-T7RBS-H6-0ZSortA			242	0.0	866			S425
1826	O425-2	O425		10-07	18:30	Silvan	Growth	1230C2	Qiagen miniprep kit	Plasmid	425	425	pSB1C3-T7RBS-H6-0ZSortA			211	0.0	867			S425
1827	O425-3	O425		10-07	18:30	Silvan	Growth	1230C3	Qiagen miniprep kit	Plasmid	425	425	pSB1C3-T7RBS-H6-0ZSortA			325	0.0	868			S425
1828	O177-4	O177		10-07	18:30	Silvan	Growth	1231C1	Qiagen miniprep kit	Plasmid	177	177	pSB1C3-2ZSortA-H6			287	0.0	869			A177#4	IC5227, IC5232	ok, ok
1829	O177-5	O177		10-07	18:30	Silvan	Growth	1231C2	Qiagen miniprep kit	Plasmid	177	177	pSB1C3-2ZSortA-H6			299	0.0	870			A177#4	IC5228, IC5233	ok, ok
1830	O177-6	O177		10-07	18:30	Silvan	Growth	1231C3	Qiagen miniprep kit	Plasmid	177	177	pSB1C3-2ZSortA-H6			505	0.0	871			A177#4
1831	O426	O426		10-07	18:30	Silvan	Growth	1232C1	Qiagen miniprep kit	Plasmid	426	426	pSB1C3-H6-Smt3-mDNMT1(731-1602)			279	0.0	872			A426	IC5229, IC5234, IC5236, DD1482	is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP, is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP, no result, is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP
1832	O426-2	O426		10-07	18:30	Silvan	Growth	1232C2	Qiagen miniprep kit	Plasmid	426	426	pSB1C3-H6-Smt3-mDNMT1(731-1602)			323	0.0	873			A426	IC5230, IC5235, DD1481, DD1483	ok, ok, ok, ok
1833	O426-3	O426		10-07	18:30	Silvan	Growth	1232C3	Qiagen miniprep kit	Plasmid	426	426	pSB1C3-H6-Smt3-mDNMT1(731-1602)			411	0.0	874			A426
1834	R561	R561		10-07	23:40	Caro	Restriction	561	PCR purification kit	Restriction	561		pSBX1K3-LOV-12-DnaXC-sfGFPc (E/X)			21	-3.1
1835	R562	R562		10-07	23:40	Caro	Restriction	562	PCR purification kit	Restriction	562		pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 (E/X)			18	0.0
1836	R563	R563		10-07	23:40	Caro	Restriction	563	PCR purification kit	Restriction	563		pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 (E/X)			3	-7.9
1837	R564	R564		10-07	23:40	Caro	Gel	458-2	Gel extraction	Restriction	564		pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 (E/S)			4	-4.8
1838	O394	O394		10-08	18:00	Caro	Growth	1214C5	Qiagen miniprep kit	Plasmid	394	394	pSBX1K3-H6-LOV-12-DnaXC-mRFP_coex				0.0				S394	DD2689, DD2688	ok, good, ok, good
1839	O260-6	O260		10-10	17:00	Caro	Growth	1245C1	Qiagen miniprep kit	Plasmid	260	260	pSBX1K3-BBa_I13507-GSGIDECHT			121	0.0	885			O260-5	DD2690, DD2691	,
1840	O260-7	O260		10-10	17:00	Caro	Growth	1245C2	Qiagen miniprep kit	Plasmid	260	260	pSBX1K3-BBa_I13507-GSGIDECHT			145	0.0	886			O260-5	DD2692, DD2693	,
1841	O261-4	O261		10-10	17:00	Caro	Growth	1246C1	Qiagen miniprep kit	Plasmid	261	261	pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha			137	0.0	890			O261-3	DD2694, DD2695	,
1842	O261-5	O261		10-10	17:00	Caro	Growth	1246C2	Qiagen miniprep kit	Plasmid	261	261	pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha			107	0.0	891			O261-3	DD2696, DD2697	,
1843	O261-6	O261		10-10	17:00	Caro	Growth	1256C1	Qiagen miniprep kit	Plasmid	261	261	pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha			107	0.0	892			O261-3	DD2698, DD2699	,
1844	R85-2	R85		10-08	21:00	MaxW	Oligos	41,42	Annealing	Restriction	85		T7RBS_BioBrick_v2	H2O	500.0	3	499.1
1845	R179	R179		08-10		Anna	Oligos	229,232	Annealing	Restriction	179		Dnmt1_Substrate_fullymethylated				0.0
1846	R180	R180		08-10		Anna	Oligos	230,231	Annealing	Restriction	180		Dnmt1_Substrate_unmethylated				0.0
1847	R181	R181		08-10		Anna	Oligos	227,228	Annealing	Restriction	181		Dnmt1_Substrate_1stMedCsemimethylated				0.0
1848	R182	R182		08-10		Anna	Oligos	227,230	Annealing	Restriction	182		Dnmt1_Substrate_1stMedChemimethylated				0.0
1849	N1	N1				Silvan	Lysis	1	ManualHis	Protein	1		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1850	N2	N2				Silvan	Lysis	3	ManualHis	Protein	2		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1851	N3	N3				Silvan	Lysis	4	ManualHis	Protein	3		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1852	N4	N4				Silvan	Lysis	5	ManualHis	Protein	4		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1853	N5	N5				Silvan	Lysis	6	ManualHis	Protein	5		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1854	N6	N6				Silvan	Lysis	7	Profinia	Protein	6		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1855	N7	N7				Silvan	Lysis	8	ManualHis	Protein	7		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1856	N8	N8				Silvan	Lysis	9	ManualHis	Protein	8		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1857	N9	N9				Silvan	Lysis	10	ManualHis	Protein	9		?	10 mM Tris, 100 mM potassium phosphate, 200 mM NaCl, 300 mM imidazole, pH 8.0			0.0	16
1858	N10	N10				Constantin, Silvan	Lysis	46	Profinia	Protein	10		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1859	N11	N11				Constantin, Silvan	Lysis	47	Profinia	Protein	11		?	50 mM Tris, 1 M NaCl, 250 mM imidazole, pH 8.0			0.0
1860	N1-2	N1				Silvan	Purification	N1/7	Dialysis	Protein	1		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1861	N2-2	N2				Silvan	Purification	N2/10	Dialysis	Protein	2		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1862	N2-3	N2				Silvan	Purification	N2/10	Dialysis	Protein	2		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0		10 Units SUMO Protease were added to the affinity-purified protein before Dialysis
1863	N3-2	N3				Silvan	Purification	N3/10	Dialysis	Protein	3		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1864	N4-2	N4				Silvan	Purification	N4/4	Dialysis	Protein	4		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1865	N5-2	N5				Silvan	Purification	N5/10	Dialysis	Protein	5		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1866	N6-2	N6				Silvan	Purification	N6/10	Dialysis	Protein	6		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1867	N7-2	N7				Silvan	Purification	N7/10	Dialysis	Protein	7		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1868	N8-2	N8				Silvan	Purification	N8/10	Dialysis	Protein	8		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1869	N9-2	N9				Silvan	Purification	N9/10	Diafiltration	Protein	9		?	20 mM Tris, 150 mM NaCl, pH 8.0			0.0
1870	N10-2	N10				Silvan	Purification	N10/4	Diafiltration	Protein	10		?	50 mM sodium phosphate, 0.675 M NaCl, pH 6.0			0.0
1871	N11-2	N11				Silvan	Purification	N11/4	Diafiltration	Protein	11		?	50 mM sodium phosphate, 0.675 M NaCl, pH 6.0			0.0
1872	N1-3	N1				Silvan	Purification	N1-2	Concentration	Protein	1		?				0.0
1873	N2-4	N2				Silvan	Purification	N2-2	Concentration	Protein	2		?				0.0
1874	N2-5	N2				Silvan	Purification	N2-3	Concentration	Protein	2		?				0.0
1875	N3-3	N3				Silvan	Purification	N3-2	Concentration	Protein	3		?				0.0
1876	N4-3	N4				Silvan	Purification	N4-2	Concentration	Protein	4		?				0.0
1877	N5-3	N5				Silvan	Purification	N5-2	Concentration	Protein	5		?				0.0
1878	N6-3	N6				Silvan	Purification	N6-2	Concentration	Protein	6		?				0.0
1879	N7-3	N7				Silvan	Purification	N7-2	Concentration	Protein	7		?				0.0
1880	N8-3	N8				Silvan	Purification	N8-2	Concentration	Protein	8		?				0.0
1881	N1-4	N1				Silvan	Purification	N1/8	Dialysis	Protein	1		?	20 mM Tris, 200 mM NaCl, 5 mM dithiothreitol, pH 7.5			0.0
1882	0-6	0									0		?				0.0
1883	0-7	0									0		?				0.0
1884	0-8	0									0		?				0.0
1885	0-9	0									0		?				0.0
1886	0-10	0									0		?				0.0
1887	0-11	0									0		?				0.0
1888	0-12	0									0		?				0.0
1889	0-13	0									0		?				0.0
1890	0-14	0									0		?				0.0
1891	0-15	0									0		?				0.0
1892	0-16	0									0		?				0.0
1893	0-17	0									0		?				0.0
1894	0-18	0									0		?				0.0
1895	0-19	0									0		?				0.0
1896	0-20	0									0		?				0.0
1897	0-21	0									0		?				0.0
1898	0-22	0									0		?				0.0
1899	0-23	0									0		?				0.0
1900	0-24	0									0		?				0.0
1901	0-25	0									0		?				0.0
1902	0-26	0									0		?				0.0
1903	0-27	0									0		?				0.0
1904	0-28	0									0		?				0.0
1905	0-29	0									0		?				0.0
1906	0-30	0									0		?				0.0
1907	0-31	0									0		?				0.0
1908	0-32	0									0		?				0.0
1909	0-33	0									0		?				0.0
1910	0-34	0									0		?				0.0
1911	0-35	0									0		?				0.0
1912	0-36	0									0		?				0.0
1913	0-37	0									0		?				0.0
1914	0-38	0									0		?				0.0
1915	0-39	0									0		?				0.0
1916	0-40	0									0		?				0.0
1917	0-41	0									0		?				0.0
1918	0-42	0									0		?				0.0
1919	0-43	0									0		?				0.0
1920	0-44	0									0		?				0.0
1921	0-45	0									0		?				0.0
1922	0-46	0									0		?				0.0
1923	0-47	0									0		?				0.0
1924	0-48	0									0		?				0.0
1925	0-49	0									0		?				0.0
1926	0-50	0									0		?				0.0
1927	0-51	0									0		?				0.0
1928	0-52	0									0		?				0.0
1929	0-53	0									0		?				0.0
1930	0-54	0									0		?				0.0
1931	0-55	0									0		?				0.0
1932	0-56	0									0		?				0.0
1933	0-57	0									0		?				0.0
1934	0-58	0									0		?				0.0
1935	0-59	0									0		?				0.0
1936	0-60	0									0		?				0.0
1937	0-61	0									0		?				0.0
1938	0-62	0									0		?				0.0
1939	0-63	0									0		?				0.0
1940	0-64	0									0		?				0.0
1941	0-65	0									0		?				0.0
1942	0-66	0									0		?				0.0
1943	0-67	0									0		?				0.0
1944	0-68	0									0		?				0.0
1945	0-69	0									0		?				0.0
1946	0-70	0									0		?				0.0
1947	0-71	0									0		?				0.0
1948	0-72	0									0		?				0.0
1949	0-73	0									0		?				0.0
1950	0-74	0									0		?				0.0
1951	0-75	0									0		?				0.0
1952	0-76	0									0		?				0.0
1953	0-77	0									0		?				0.0
1954	0-78	0									0		?				0.0
1955	0-79	0									0		?				0.0
1956	0-80	0									0		?				0.0
1957	0-81	0									0		?				0.0
1958	0-82	0									0		?				0.0
1959	0-83	0									0		?				0.0
1960	0-84	0									0		?				0.0
1961	0-85	0									0		?				0.0
1962	0-86	0									0		?				0.0
1963	0-87	0									0		?				0.0
1964	0-88	0									0		?				0.0
1965	0-89	0									0		?				0.0
1966	0-90	0									0		?				0.0
1967	0-91	0									0		?				0.0
1968	0-92	0									0		?				0.0
1969	0-93	0									0		?				0.0
1970	0-94	0									0		?				0.0
1971	0-95	0									0		?				0.0
1972	0-96	0									0		?				0.0
1973	0-97	0									0		?				0.0
1974	0-98	0									0		?				0.0
1975	0-99	0									0		?				0.0
1976	0-100	0									0		?				0.0
1977	0-101	0									0		?				0.0
1978	0-102	0									0		?				0.0
1979	0-103	0									0		?				0.0
1980	0-104	0									0		?				0.0
1981	0-105	0									0		?				0.0
1982	0-106	0									0		?				0.0
1983	0-107	0									0		?				0.0
1984	0-108	0									0		?				0.0
1985	0-109	0									0		?				0.0
1986	0-110	0									0		?				0.0
1987	0-111	0									0		?				0.0

Team:Heidelberg/AwesomeSheet/Purifications

From 2014.igem.org

– Purifications

Awesome Sheet PA Gels Dnmt1 Methylation Assay Blots Waste SURPREMErun Strains Stocks Samples Purifications Plate Reader Marker LIGHTRun Enzymes Competent cells Induction Growth 3AL AGels AssmProd Assembly GoldenGate Parts Submission Parts PCR products PCRs Plasmids Plates Primer Res SL Trafos