Team:Heidelberg/AwesomeSheet/Stocks
From 2014.igem.org
Lab book
– Stocks
A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | R | S | T | U | V | W | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | All glyceol stocks are in box 1 in the -50^°C | Organism | Origin | Related Prep | Related Sequenzing | Related Sequenzing (origin) | Related Induction | |||||||||||||||
2 | ID | Person | Date | Growth | Storage Buffer | ID | Species | Strain | Plasmids | PID1 | PID2 | PID3 | Resistances | Comments | ID | Result | ID | Result | Result1 | Result 2 | ||
3 | 1 | Jan | 06-05 | 1C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | #N/A | #N/A | ||||||||||||
4 | 2 | Jan | 06-05 | 2C1 | 25% glycerol in LB | 2 | E. coli | DH10beta | 2 | #N/A | ||||||||||||
5 | 3 | Jan | 06-05 | 3C1 | 25% glycerol in LB | 3 | E. coli | Turbos | #N/A | |||||||||||||
6 | 4 | Jan | 06-05 | 5C1 | 25% glycerol in LB | 5 | E. coli | One Shot | #N/A | |||||||||||||
7 | 5 | Jan | 06-05 | 4C1 | 25% glycerol in LB | 4 | E. coli | MG1655 | #N/A | |||||||||||||
8 | 6 | Jan | 06-05 | 6C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | #N/A | |||||||||||||
9 | 7 | Silvan, Jakob and Max W. | 06-07 | 43C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-3-17E | O7 | pSB1C3-BBa_I15016 | 7 | Cm | BF8247 | was previously thought to be BBa_C0012 | #N/A | #N/A | |||||
10 | 8 | Silvan, Jakob and Max W. | 06-07 | 42C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-3-4G | O6 | pSB1C3-BBa_R0010 | 6 | Cm | BF8249 | ok | #N/A | #N/A | |||||
11 | 9 | Silvan, Jakob and Max W. | 06-07 | 10C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O12-2 | O12 | pUC19 | 12 | Amp | |||||||||
12 | 10 | Silvan, Jakob and Max W. | 06-07 | 40C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-2-2N | O4 | pSB1C3-BBa_C0012 | 4 | Cm | BF8250 | was prevoiously thought to be BBa_I15016 | #N/A | #N/A | |||||
13 | 11 | Jakob, Jan | 06-08 | 37C2 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-4-19D | O1 | BBa_J61002-BBa_J23110 | 1 | Amp | BF8244 | ok | #N/A | #N/A | |||||
14 | 12 | Jakob, Jan | 06-08 | 38C2 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-4-21B | O2 | BBa_J61002-BBa_J23118 | 2 | Amp | BF8245 | ok | #N/A | #N/A | |||||
15 | 13 | Jakob, Jan | 06-08 | 39C2 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-4-17D | O3 | BBa_J61002-BBa_J23100 | 3 | Amp | BF8246 | ok | #N/A | #N/A | |||||
16 | 14 | Jakob, Jan | 06-08 | 41C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-2-24B | O5 | pSB1C3-BBa_E0240 | 5 | Cm | BF8248 | insertion before prefix leading to additional EcoRI site | #N/A | #N/A | |||||
17 | 15 | Jakob, Jan | 06-08 | 44C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-3-17G | O8 | pSB1C3-BBa_I15017 | 8 | Cm | BF8251 | ok | #N/A | #N/A | |||||
18 | 16 | Jakob | 06-12 | 10 | 25% glycerol in LB | 5 | E. coli | One Shot | O13 | pSB1C3-pKH6insert | 13 | Cm | BF8252, BF8253 | ok, ok | ||||||||
19 | 17 | Jakob | 06-12 | 11 | 25% glycerol in LB | 5 | E. coli | One Shot | O14 | pSB1C3-pKH7insert | 14 | Cm | BF8253, BF8254 | ok, ok | ||||||||
20 | 18 | Jakob | 06-12 | 12 | 25% glycerol in LB | 5 | E. coli | One Shot | O15 | pSB1C3-pKH18insert | 15 | Cm | BF8254, BF8255 | negativ, araC, negativ, Shewanella violacea | ||||||||
21 | 19 | Silvan | 06-21 | 52C1 | 25% glycerol in LB | O16 | pGBMCS-SortA | 16 | Amp | II2247 | ok | |||||||||||
22 | 20 | Silvan | 06-21 | 53C1 | 25% glycerol in LB | O17 | pHYRSF1 | 17 | Kan | II2232 | ok | |||||||||||
23 | 21 | Silvan | 06-21 | 54C1 | 25% glycerol in LB | O18 | pHYRSF1-02 | 18 | Kan | II2233 | ok | |||||||||||
24 | 22 | Silvan | 06-21 | 55C1 | 25% glycerol in LB | O19 | pJJDuet30 | 19 | Kan | II2234 | Primer hat an 2 Stellen gebunden, sieht aber ok aus | |||||||||||
25 | 23 | Silvan | 06-21 | 56C1 | 25% glycerol in LB | O20 | pSFBAD09 | 20 | Amp | II2235 | ok | |||||||||||
26 | 24 | Maggie, Silvan | 06-25 | 57C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O22-0 | O22 | pFastBac-mDNMT1 | 22 | Amp | |||||||||
27 | 25 | Maggie, Silvan | 06-25 | 58C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O23-0 | O23 | pRSFDuet-1-mDNMT1(731-1602) | 23 | Kan | II2225, II2227, II2228, II2229 | ok, ok, ok, ok | |||||||
28 | 26 | Maggie, Silvan | 06-25 | 59C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O24-0 | O24 | pIT77 | 24 | Amp | II2236 | ok | |||||||
29 | 27 | Maggie, Silvan | 06-25 | 60C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O25-0 | O25 | pIT29 | 25 | Amp | II2237 | ok | |||||||
30 | 28 | Maggie, Silvan | 06-25 | 61C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O26-0 | O26 | pIT21 | 26 | Amp | II2238 | ok | |||||||
31 | 29 | Maggie, Silvan | 06-25 | 62C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O27-0 | O27 | pCL20 | 27 | Kan | II2239 | ok | |||||||
32 | 30 | Maggie, Silvan | 06-25 | 63C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O28-0 | O28 | pAU07 | 28 | Amp | II2240 | ok | |||||||
33 | 31 | Maggie, Silvan | 06-25 | 64C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O29-0 | O29 | pVS41 | 29 | Amp | II2241 | ok | |||||||
34 | 32 | Maggie, Silvan | 06-25 | 65C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O30-0 | O30 | pVS07 | 30 | Kan | II2242 | ok | |||||||
35 | 33 | Silvan | 07-03 | 66C1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O31 | pRARE | 31 | Cm | II2224 | ok, aber falsche Richtung | ||||||||
36 | 34 | Jan | 07-06 | 153C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | S38 | O38 | pSB1AK8-BBa_I712074-BBa_I15016 | 38 | Amp, Kan | #N/A | #N/A | |||||||
37 | 35 | Jan | 07-06 | 154C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | S39 | O39 | pSB1AK8-BBa_I712074-BBa_I15017 | 39 | Amp, Kan | #N/A | #N/A | |||||||
38 | 36 | Jan | 07-06 | 155C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | S40 | O40 | pSB1AK8-BBa_I712074-BBa_E0240 | 40 | Amp, Kan | #N/A | #N/A | |||||||
39 | 37 | Jan | 07-06 | 156C2 | 25% glycerol in LB | 1 | E. coli | TOP10 | S41 | O41 | pSB1AK8-BBa_I712074-S-BBa_J70390 | 41 | Amp, Kan | #N/A | #N/A | |||||||
40 | 38 | Jan | 07-06 | 142C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2013-5-7J | O37 | pSB1A2-BBa_S03520 | 37 | Amp | #N/A | #N/A | |||||||
41 | 39 | Caro | 7/8/2014 | 157C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A32#2 | O32-2 | pSB1C3-2ZNpuH6 | 32 | Cm | II2245, II2246 | insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part, insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part | #N/A | #N/A | |||||
42 | 40 | Silvan | 7/4/2014 | 131C1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O23 | O23-2 | pRSFDuet-1-mDNMT1(731-1602), pRARE | 23 | 31 | KanCm | II2225, II2227, II2228, II2229 | ok, ok, ok, ok | ||||||
43 | 41 | TODO! | 25% glycerol in LB | 3 | E. coli | Turbos | 55 | Amp | < insert growth id | |||||||||||||
44 | 42 | Caro | 7/8/2014 | 158C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A33 | O33 | pSB1C3-2ZNpu | 33 | Cm | II2248, II2249 | insertion in the prefix, additional His after Flag, bad sequencing | #N/A | #N/A | |||||
45 | 42b | Caro | 7/8/2014 | 158C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A33 | O33-2 | pSB1C3-2ZNpu | 33 | Cm | discard | II2252 | additional His after flag, insertion in the prefix | #N/A | #N/A | ||||
46 | 42c | Caro | 7/8/2014 | 158C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A33 | O33-3 | pSB1C3-2ZNpu | 33 | Cm | discard | II2253 | additional His after flag, insertion in the prefix | #N/A | #N/A | ||||
47 | 43 | Caro | 7/8/2014 | 134C1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O30 | pVS07, pRARE | 30 | 31 | Kan, Cm | #N/A | II2242 | ok | ||||||
48 | 44 | Caro | 7/8/2014 | 137C1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O29 | pVS41, pRARE | 29 | 31 | Amp, Cm | #N/A | II2241 | ok | ||||||
49 | 45 | Caro | 7/8/2014 | 162C1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O29 | O29,30,31-3 | pVS41, pVS07, pRARE | 29 | 30 | 31 | Amp, Kan, Cm | II2241 | ok | |||||
50 | 46 | Silvan | 07-08 | 164C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | D2014-3-12I | O43 | pSB1C3-BBa_I746907 | 43 | Cm | II2250, II2251 | , | #N/A | #N/A | |||||
51 | 47 | Jan | 07-03 | 129C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A35 | O35-2 | pSB1C3-2ZSsp | 35 | Cm | II2230, II2243 | ok, ok | #N/A | #N/A | |||||
52 | 48 | Jan | 07-11 | 66C4 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | pRARE | 31 | Cm | #N/A | ||||||||||
53 | 49 | Jan | 07-06 | 133C1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O17 | pHYRSF1, pRARE | 17 | 31 | Kan, Cm | #N/A | II2232 | ok | ||||||
54 | 50 | Jan | 07-03 | 130C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2013-5-6N | O36 | pSB1AK8-BBa_I712074 | 36 | Amp | T7 prom | II2226 | RFC23 (missing the final G in the prefix and the primary T in the suffix) | #N/A | #N/A | ||||
55 | 51 | Jan | 07-11 | 170C1-1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O39 | pSB1AK8-BBa_I712074-BBa_I15017, pRARE | 39 | 31 | Amp, Kan, Cm | #N/A | ||||||||
56 | 52 | Jan | 07-11 | 171C1-1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O40 | pSB1AK8-BBa_I712074-BBa_E0240, pRARE | 40 | 31 | Amp, Kan, Cm | #N/A | ||||||||
57 | 53 | Jan | 07-11 | 172C1-1 | 25% glycerol in LB | 7 | E. coli | Rosetta (DE3) | O41 | pSB1AK8-BBa_I712074-S-BBa_J70390, pRARE | 41 | 31 | Amp, Kan, Cm | #N/A | ||||||||
58 | 54 | Silvan | 7/12/2014 | 178C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S47#3 | O47 | pSB1AK8-T7-2ZNpu | 47 | Amp | II2263, II2265 | , | #N/A | #N/A | |||||
59 | 55 | Silvan | 7/12/2014 | 180C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | O36 | pSB1AK8-BBa_I712074 | 36 | Amp | #N/A | II2226 | RFC23 (missing the final G in the prefix and the primary T in the suffix) | |||||||
60 | 56 | Silvan | 7/21/2014 | 211C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A55 | O55 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 55 | Amp | II2267, II2268 | , | #N/A | #N/A | |||||
61 | 57 | Jan | 7/24/2014 | 209C2 | 25% glycerol in LB | 5 | E. coli | One Shot | A55 | O55-2 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 55 | Amp | II2269 | #N/A | #N/A | ||||||
62 | 58 | Jan | 7/24/2014 | 211C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A55 | O55-3 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 55 | Amp | II2270 | #N/A | #N/A | ||||||
63 | 59 | Jan | 7/24/2014 | 212C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A55#2 | O55-4 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 55 | Amp | II2271 | #N/A | #N/A | ||||||
64 | 60 | Jan | 7/24/2014 | 221C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | O53 | O53-2 | pSB1A2-BBa_I13507 | 53 | Amp | |||||||||
65 | 61 | Jan | 7/24/2014 | 218C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | O50 | O50-2 | pSB4A5-BBa_J04450 | 50 | Amp | |||||||||
66 | 62 | Jan | 7/24/2014 | 217C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | O49 | O49-3 | pSB1A3-BBa_J04450 | 49 | Amp | |||||||||
67 | 63 | Jan | 7/24/2014 | 219C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | O51 | O51-2 | pSB4C5-BBa_J04450 | 51 | Cm | |||||||||
68 | 64 | Jan | 7/24/2014 | 220C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | O52 | O52-2 | pSB4K5-BBa_J04450 | 52 | Kan | |||||||||
69 | 65 | Jan | 7/24/2014 | 222C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | O54 | O54-2 | pSB1K3-BBa_J04450 | 54 | Kan | |||||||||
70 | 66 | Jan | 7/24/2014 | 227C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O64-2 | O64-4 | pSB1T3-BBa_J04450 | 64 | Tet | |||||||||
71 | 67 | Jan | 7/24/2014 | 224C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-4-8D | O66 | pSB3T5-BBa_J04450 | 66 | Tet | #N/A | #N/A | |||||||
72 | 68 | Jan | 07-26 | 234C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L57#5 | O57-2 | pSBX1A3-BBa_I13507 | 57 | Amp | FG1165, FG1198 | sequenzing starts ok, then mixture?, ok | #N/A | #N/A | |||||
73 | 69 | Jan | 07-26 | 235C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L58#3 | O58-3 | pSBX1C3-BBa_I13507 | 58 | Cm | #N/A | #N/A | |||||||
74 | 70 | Jan | 07-26 | 236C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L59#5 | O59-3 | pSBX1K3-BBa_I13507 | 59 | Kan | FG1167, FG1177, FG1199 | frameshift, two fucking huge insertions, ok,longest, | #N/A | #N/A | |||||
75 | 71 | Jan | 07-26 | 238C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L61#3 | O61 | pSBX4A5-BBa_I13507 | 61 | Amp | FG1169 | starts ok then mixture | #N/A | #N/A | |||||
76 | 72 | Jan | 07-26 | 239C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L62#3 | O62 | pSBX4C5-BBa_I13507 | 62 | Cm | FG1170 | ok | #N/A | #N/A | |||||
77 | 73 | Jan | 07-26 | 240C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L63#3 | O63 | pSBX4K5-BBa_I13507 | 63 | Kan | FG1187 | bad sequencing | #N/A | #N/A | |||||
78 | 74 | Jan | 07-26 | 234C2 | 25% glycerol in LB | 3 | E. coli | Turbos | L57#5 | O57-3 | pSBX1A3-BBa_I13507 | 57 | Amp | FG1173 | bad sequencing | #N/A | #N/A | |||||
79 | 75 | Jan | 07-26 | 235C2 | 25% glycerol in LB | 3 | E. coli | Turbos | L58#3 | O58-4 | pSBX1C3-BBa_I13507 | 58 | Cm | FG1175 | ok | #N/A | #N/A | |||||
80 | 76 | Jan | 07-26 | 236C2 | 25% glycerol in LB | 3 | E. coli | Turbos | L59#5 | O59-4 | pSBX1K3-BBa_I13507 | 59 | Kan | FG1178 | ok | #N/A | #N/A | |||||
81 | 77 | Jan | 07-26 | 238C2 | 25% glycerol in LB | 3 | E. coli | Turbos | L61#3 | O61-2 | pSBX4A5-BBa_I13507 | 61 | Amp | FG1181 | bad sequencing | #N/A | #N/A | |||||
82 | 78 | Jan | 07-26 | 239C2 | 25% glycerol in LB | 3 | E. coli | Turbos | L62#3 | O62-2 | pSBX4C5-BBa_I13507 | 62 | Cm | #N/A | #N/A | |||||||
83 | 79 | Jan | 07-26 | 240C2 | 25% glycerol in LB | 3 | E. coli | Turbos | L63#3 | O63-2 | pSBX4K5-BBa_I13507 | 63 | Kan | FG1171 | no sequenzing | #N/A | #N/A | |||||
84 | 80 | Jan | 07-26 | 234C3 | 25% glycerol in LB | 3 | E. coli | Turbos | L57#5 | O57-4 | pSBX1A3-BBa_I13507 | 57 | Amp | FG1174 | bad sequencing | #N/A | #N/A | |||||
85 | 81 | Jan | 07-26 | 235C3 | 25% glycerol in LB | 3 | E. coli | Turbos | L58#3 | O58-5 | pSBX1C3-BBa_I13507 | 58 | Cm | FG1166 | bad sequenzing | #N/A | #N/A | |||||
86 | 82 | Jan | 07-26 | 236C3 | 25% glycerol in LB | 3 | E. coli | Turbos | L59#5 | O59-5 | pSBX1K3-BBa_I13507 | 59 | Kan | FG1179 | ok, short read | #N/A | #N/A | |||||
87 | 83 | Jan | 07-26 | 238C3 | 25% glycerol in LB | 3 | E. coli | Turbos | L61#3 | O61-3 | pSBX4A5-BBa_I13507 | 61 | Amp | FG1182 | bad sequencing | #N/A | #N/A | |||||
88 | 84 | Jan | 07-26 | 239C3 | 25% glycerol in LB | 3 | E. coli | Turbos | L62#3 | O62-3 | pSBX4C5-BBa_I13507 | 62 | Cm | #N/A | #N/A | |||||||
89 | 85 | Jan | 07-26 | 240C3 | 25% glycerol in LB | 3 | E. coli | Turbos | L63#3 | O63-3 | pSBX4K5-BBa_I13507 | 63 | Kan | FG1188 | bad sequencing | #N/A | #N/A | |||||
90 | 86 | Silvan | 07-31 | 263C1-2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O48 | pSB1AK8-T7-2ZNpu:(GFP-H6) | 48 | Amp | #N/A | II2264, II2266 | , | |||||||
91 | 87 | Charlotte | 07-31 | 279C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S80 | O80 | pSBX4C5-2ZSspH6:GFP | 80 | Cm | #N/A | #N/A | |||||||
92 | 88 | Charlotte | 07-31 | 280C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S81 | pSBX4C5-2ZNpuH6:GFP | 81 | Cm | #N/A | #N/A | #N/A | |||||||
93 | 89 | Caro | 08-02 | 297C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | S77 | O77 | pSBX1C3-T7RBS_BBv2 | 77 | Cm | #N/A | #N/A | |||||||
94 | 90 | Caro | 08-02 | 289C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S78 | O78 | pSBX1K3-T7RBS_BBv2 | 78 | Kan | 76DB35 | seems ok; some bases badly called | #N/A | #N/A | |||||
95 | 91 | Caro | 08-02 | 289C2 | 25% glycerol in LB | 3 | E. coli | Turbos | S78 | O78-2 | pSBX1K3-T7RBS_BBv2 | 78 | Kan | #N/A | #N/A | |||||||
96 | 92 | Caro | 08-02 | 289C3 | 25% glycerol in LB | 3 | E. coli | Turbos | S78 | O78-3 | pSBX1K3-T7RBS_BBv2 | 78 | Kan | #N/A | #N/A | |||||||
97 | 93 | Caro | 08-02 | 285C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S72#2 | O72 | pSBX4A5-T7RBS-IGEMHD_v3 | 72 | Amp | FG1191 | good sequencing, ok | #N/A | #N/A | |||||
98 | 94 | Caro | 08-02 | 294C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | S72#2 | O72-2 | pSBX4A5-T7RBS-IGEMHD_v3 | 72 | Amp | #N/A | #N/A | |||||||
99 | 95 | Caro | 08-02 | 292C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | S70#2 | O70 | pSBX1K3-T7RBS-IGEMHD_v3 | 70 | Kan | #N/A | #N/A | |||||||
100 | 96 | Caro | 08-02 | 292C2 | 25% glycerol in LB | 1 | E. coli | TOP10 | S70#2 | O70-2 | pSBX1K3-T7RBS-IGEMHD_v3 | 70 | Kan | #N/A | #N/A | |||||||
101 | 97 | Charlotte | 08-02 | 299C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O80 | pSBX4C5-2ZSspH6:GFP | 80 | Cm | #N/A | |||||||||
102 | 98 | Anna | 08-03 | 242C13 | 25% glycerol in LB | 3 | E. coli | Turbos | L60#3 | O60-3 | pSBX1T3-BBa_I13507 | 60 | Tet | FG1180 | ok | #N/A | #N/A | |||||
103 | 99 | Anna | 08-03 | 327C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O62 | O62-5 | pSBX4C5-BBa_I13507 | 62 | Cm | keine Expression, wurde vernichtet | FG1170 | ok | ||||||
104 | 100 | Anna | 08-03 | 327C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O62 | O62-6 | pSBX4C5-BBa_I13507 | 62 | Cm | Funktionierender Expressionsvektor | FG1184 | ok, longest | FG1170 | ok | ||||
105 | 101 | Anna | 08-03 | 327C3 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O62 | O62-7 | pSBX4C5-BBa_I13507 | 62 | Cm | keine Expression, wurde vernichtet | FG1170 | ok | ||||||
106 | 102 | Anna | 08-03 | 327C4 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O62 | O62-8 | pSBX4C5-BBa_I13507 | 62 | Cm | keine Expression, wurde vernichtet | FG1170 | ok | ||||||
107 | 103 | Anna | 08-03 | 327C5 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O62 | O62-9 | pSBX4C5-BBa_I13507 | 62 | Cm | Funktionierender Expressionsvektor | FG1185 | ok | FG1170 | ok | ||||
108 | 104 | Anna | 08-03 | 327C6 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O62 | O62-10 | pSBX4C5-BBa_I13507 | 62 | Cm | Funktionierender Expressionsvektor | FG1186 | ok | FG1170 | ok | ||||
109 | 105 | Jan | 08-12 | 225C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-4-8B | pSB1T3-BBa_J04450 | 64 | Tet | #N/A | #N/A | #N/A | |||||||
110 | 106 | Silvan | 08-18 | 448C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O55-5 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 55 | Amp | II2281 | |||||||||
111 | 107 | Silvan | 08-18 | 448C2 | 25% glycerol in LB | 3 | E. coli | Turbos | O55-6 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 55 | Amp | II2282 | |||||||||
112 | 108 | Lisa | 08-05 | 348C2 | 25% glycerol in LB | 1 | E. coli | TOP10 | O45-5 | O45-8 | pSB1C3-2ZNpuH6:GFP | 45 | Cm | II2257, II2256 | no sequencing, we realized that this is npu (O45), has a mutation A1197del | |||||||
113 | 109 | Lisa | 08-05 | 349C1 | 25% glycerol in LB | 1 | E. coli | TOP10 | O36 | O36-3 | pSB1AK8-BBa_I712074 | 36 | Amp, Kan | II2226 | RFC23 (missing the final G in the prefix and the primary T in the suffix) | |||||||
114 | 110 | Lisa | 08-05 | 347C2 | 25% glycerol in LB | 1 | E. coli | TOP10 | O44-4 | O44-8 | pSB1C3-2ZSspH6:GFP | 44 | Cm | II2255, II2254 | no sequencing, we realized that "ist glaube ich Ssp_DnaE. hat funktioniert", besides that ok | |||||||
115 | 111 | Jan | 08-05 | 356C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O17 | pHYRSF1 | 17 | Kan | was standing on the bench for a day | #N/A | II2232 | ok | ||||||
116 | 112 | Jan | 08-05 | 357C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O18 | pHYRSF1-02 | 18 | Kan | was standing on the bench for a day | #N/A | II2233 | ok | ||||||
117 | 113 | Jan | 08-05 | 358C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O29 | pVS41 | 29 | Amp | was standing on the bench for a day | #N/A | II2241 | ok | ||||||
118 | 114 | Jan | 08-05 | 359C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O30 | pVS07 | 30 | Kan | was standing on the bench for a day | #N/A | II2242 | ok | ||||||
119 | 115 | Jan | 08-05 | 360C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O29 | pVS41, pHYRSF1 | 29 | 17 | Amp, Kan | was standing on the bench for a day | #N/A | II2241 | ok | |||||
120 | 116 | Jan | 08-05 | 361C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O29 | pVS41, pVS07 | 29 | 30 | Amp, Kan | was standing on the bench for a day | #N/A | II2241 | ok | |||||
121 | 117 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
122 | 118 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
123 | 119 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
124 | 120 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
125 | 121 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
126 | 122 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
127 | 123 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
128 | 124 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
129 | 125 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
130 | 126 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
131 | 127 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
132 | 128 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
133 | 129 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
134 | 130 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
135 | 131 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
136 | 132 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
137 | 133 | Anna | 08-07 | 384C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O59-3 | O59-6 | pSBX1K3-BBa_I13507 | 59 | Kan | FG1167, FG1177, FG1199 | frameshift, two fucking huge insertions, ok,longest, | |||||||
138 | 134 | Anna | 08-07 | 384C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O59-3 | O59-7 | pSBX1K3-BBa_I13507 | 59 | Kan | negativ | FG1167, FG1177, FG1199 | frameshift, two fucking huge insertions, ok,longest, | ||||||
139 | 135 | Anna | 08-07 | 384C3 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O59-3 | O59-8 | pSBX1K3-BBa_I13507 | 59 | Kan | FG1167, FG1177, FG1199 | frameshift, two fucking huge insertions, ok,longest, | |||||||
140 | 136 | Anna | 08-07 | 385C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O59-5 | O59-9 | pSBX1K3-BBa_I13507 | 59 | Kan | FG1179 | ok, short read | |||||||
141 | 137 | Anna | 08-07 | 385C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O59-5 | O59-10 | pSBX1K3-BBa_I13507 | 59 | Kan | FG1179 | ok, short read | |||||||
142 | 138 | Anna | 08-07 | 385C3 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O59-5 | O59-11 | pSBX1K3-BBa_I13507 | 59 | Kan | FG1179 | ok, short read | |||||||
143 | 139 | Anna | 08-07 | 386C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O61-4 | pSBX4A5-BBa_I13507 | 61 | Amp | nicht exprimierend | #N/A | FG1183 | ok | ||||||
144 | 140 | Anna | 08-07 | 386C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O61-4 | O61-5 | pSBX4A5-BBa_I13507 | 61 | Amp | FG1183 | ok | |||||||
145 | 141 | Anna | 08-07 | 386C3 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O61-4 | O61-6 | pSBX4A5-BBa_I13507 | 61 | Amp | FG1183 | ok | |||||||
146 | 142 | Anna | 08-07 | 387C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O63-4 | O63-5 | pSBX4K5-BBa_I13507 | 63 | Kan | nicht exprimierend | FG1189 | ok | ||||||
147 | 143 | Anna | 08-07 | 387C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O63-4 | pSBX4K5-BBa_I13507 | 63 | Kan | #N/A | FG1189 | ok | |||||||
148 | 144 | Anna | 08-07 | 387C3 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O63-4 | O63-6 | pSBX4K5-BBa_I13507 | 63 | Kan | FG1189 | ok | |||||||
149 | 148 | 08-10 | 300C4 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O80-2 | O80-3 | pSBX4C5-2ZSspH6:GFP | 80 | Cm | FG1203, FG1208 | ok, ok | ||||||||
150 | 149 | 08-10 | 301C3 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O81 | O81-2 | pSBX4C5-2ZNpuH6:GFP | 81 | Cm | FG1204, FG1209 | pSBX4C5-2ZSspH6:GFP, pSBX4C5-2ZSspH6:GFP | ||||||||
151 | 150 | 08-10 | 372C4 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | S86 | O86 | pSB1AK8-BBa_I712074-2ZSspH6:GFP | 86 | Amp, Kan | FG1205, FG1210 | is just pSB1AK8-BBa_I712074, is just pSB1AK8-BBa_I712074 | #N/A | #N/A | ||||||
152 | 151 | 08-10 | 351C7 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | S87 | O87 | pSB1AK8-BBa_I712074-2ZNpuH6:GFP | 87 | Amp, Kan | FG1206, FG1211 | mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown, mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown | #N/A | #N/A | ||||||
153 | 152 | 08-13 | 423C2 | 25% glycerol in LB | 3 | E. coli | Turbos | O102-2 | O102-3 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 102 | Kan | FG1200, II2274 | ok, ok | ||||||||
154 | 153 | 08-13 | 423C3 | 25% glycerol in LB | 3 | E. coli | Turbos | O102-2 | O102-4 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 102 | Kan | FG1201, II2275 | ok, ok | ||||||||
155 | 154 | 08-13 | 423C4 | 25% glycerol in LB | 3 | E. coli | Turbos | O102-2 | O102-5 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 102 | Kan | ||||||||||
156 | 155 | 08-13 | 424C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A102 | O102-6 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 102 | Kan | #N/A | #N/A | ||||||||
157 | 156 | 08-13 | 424C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A102 | O102-7 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 102 | Kan | FG1202, II2276 | ok, ok | #N/A | #N/A | ||||||
158 | 157 | 08-14 | 433C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A103 | O103 | pSBX1K3-2Zv2Npu | 103 | Kan | charlies zeug, falsch | FG1213, FG1214 | wrong, charlie's stuff, wrong, charlie's stuff | #N/A | #N/A | |||||
159 | 158 | 08-14 | 434C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-4-5C | O104 | pSB1C3-BBa_I746908 | 104 | Cm | sfGFP | II2277, II2278 | ok, ok | #N/A | #N/A | |||||
160 | 159 | 08-14 | 433C4 | 25% glycerol in LB | 3 | E. coli | Turbos | A103 | O103-2 | pSBX1K3-2Zv2Npu | 103 | Kan | II2279, II2280 | ok (silent mutation in selection marker), ok (silent mutation in selection marker) | #N/A | #N/A | ||||||
161 | 160 | 08-16 | 436C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O102-3 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 102 | Kan | #N/A | FG1200, II2274 | ok, ok | ||||||||
162 | 161 | Caro | 08-16 | Bsu1-1 | 25% glycerol in LB | 8 | Bacillus subtilis | 168 | #N/A | |||||||||||||
163 | 162 | 08-16 | 442C2 | 25% glycerol in LB | 3 | E. coli | Turbos | O73 | pSBX4C5-T7RBS-IGEMHD_v3 | 73 | Cm | #N/A | FG1192 | good sequencing, ok | ||||||||
164 | 163 | 08-16 | 442C2-1 | 25% glycerol in LB | 3 | E. coli | Turbos | O73 | pSBX4C5-T7RBS-IGEMHD_v3 | 73 | Cm | #N/A | FG1192 | good sequencing, ok | ||||||||
165 | 164 | 08-17 | 438C8 | 25% glycerol in LB | 3 | E. coli | Turbos | S98 | O98-3 | pSBX4C5-2Zv2Npu | 98 | Cm | discard | #N/A | #N/A | |||||||
166 | 165 | 08-17 | 438C9 | 25% glycerol in LB | 3 | E. coli | Turbos | S98 | O98-4 | pSBX4C5-2Zv2Npu | 98 | Cm | II2273 | ok | #N/A | #N/A | ||||||
167 | 166 | 08-17 | 441C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O71-2 | pSBX1T3-T7RBS-IGEMHD_v3 | 71 | Tet | #N/A | FG1190 | good sequencing, ok | ||||||||
168 | 167 | 08-17 | 441C1-1 | 25% glycerol in LB | 3 | E. coli | Turbos | O71-2 | pSBX1T3-T7RBS-IGEMHD_v3 | 71 | Tet | #N/A | FG1190 | good sequencing, ok | ||||||||
169 | 168 | 08-17 | 443C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A103#2 | O103-3 | pSBX1K3-2Zv2Npu | 103 | Kan | tatsächlich richtig | #N/A | #N/A | |||||||
170 | 169 | 8/17/2014 | 444C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G110 | O110 | pSBX1K3-2Zv2Npu:(LambdaLys-H6) | 110 | Kan | #N/A | #N/A | ||||||||
171 | 170 | 08-17 | 445C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G111 | O111 | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) | 111 | Kan | #N/A | #N/A | ||||||||
172 | 171 | 08-17 | 446C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G112 | O112 | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His | 112 | Kan | #N/A | #N/A | ||||||||
173 | 172 | 08-17 | 447C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G113 | O113 | pSBX1K3-2Zv2Npu:Xyla_circ_noHis | 113 | Kan | #N/A | #N/A | ||||||||
174 | 173 | Silvan | 08-17 | 448C3 | 25% glycerol in LB | 3 | E. coli | Turbos | O55-7 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 55 | Amp | II2283 | |||||||||
175 | 174 | Silvan | 08-17 | 448C4 | 25% glycerol in LB | 3 | E. coli | Turbos | O55-8 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 55 | Amp | II2284 | |||||||||
176 | 175 | Silvan | 08-18 | 453C3 | 25% glycerol in LB | 3 | E. coli | Turbos | S116 | O116 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 116 | Kan | II2285 | #N/A | #N/A | ||||||
177 | 176 | Silvan | 08-18 | 454C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S116#2 | O116-2 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 116 | Kan | II2286 | single base deletion | #N/A | #N/A | |||||
178 | 177 | Silvan | 08-18 | 455C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S116#3 | O116-3 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 116 | Kan | II2287 | two-base deletion and point mutation | #N/A | #N/A | |||||
179 | 178 | Silvan | 08-18 | 455C3 | 25% glycerol in LB | 3 | E. coli | Turbos | S116#3 | O116-4 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 116 | Kan | #N/A | #N/A | |||||||
180 | 179 | Lisa | 08-19 | 458C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-2-7E | O109 | pSB1C3-BBa_K808000 | 109 | Cm | II2288 | ok | #N/A | #N/A | |||||
181 | 180 | Lisa | 08-19 | 458C2 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-2-7E | O109-2 | pSB1C3-BBa_K808000 | 109 | Cm | discard | #N/A | #N/A | ||||||
182 | 181 | Lisa | 08-19 | 458C3 | 25% glycerol in LB | 3 | E. coli | Turbos | D2014-2-7E | O109-3 | pSB1C3-BBa_K808000 | 109 | Cm | discard | #N/A | #N/A | ||||||
183 | 182 | Lisa | 08-19 | 467C1 | 25% glycerol in LB | 3 | E. coli | Turbos | D2013-2-17M | O117 | pSB1C3-BBa_K741002 | 117 | Cm | discard | #N/A | #N/A | ||||||
184 | 183 | Lisa | 08-19 | 467C2 | 25% glycerol in LB | 3 | E. coli | Turbos | D2013-2-17M | O117-2 | pSB1C3-BBa_K741002 | 117 | Cm | II2289 | ok | #N/A | #N/A | |||||
185 | 184 | Lisa | 08-19 | 467C3 | 25% glycerol in LB | 3 | E. coli | Turbos | D2013-2-17M | O117-3 | pSB1C3-BBa_K741002 | 117 | Cm | discard | #N/A | #N/A | ||||||
186 | 185 | Anna | 08-20 | 383C4 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O58-4 | pSBX1C3-BBa_I13507 | 58 | Cm | #N/A | FG1175 | ok | |||||||
187 | 186 | Anna | 08-20 | 383C5 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O58-4 | pSBX1C3-BBa_I13507 | 58 | Cm | #N/A | FG1175 | ok | |||||||
188 | 187 | Anna | 08-20 | 383C6 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O58-4 | pSBX1C3-BBa_I13507 | 58 | Cm | #N/A | FG1175 | ok | |||||||
189 | 188 | Anna | 08-20 | 383C7 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O58-4 | pSBX1C3-BBa_I13507 | 58 | Cm | #N/A | FG1175 | ok | |||||||
190 | 189 | Anna | 08-20 | 383C8 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O58-4 | pSBX1C3-BBa_I13507 | 58 | Cm | #N/A | FG1175 | ok | |||||||
191 | 190 | Anna | 08-20 | 418C6 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O60-4 | pSBX1T3-BBa_I13507 | 60 | Tet | #N/A | |||||||||
192 | 191 | Anna | 08-20 | 418C7 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O60-4 | pSBX1T3-BBa_I13507 | 60 | Tet | #N/A | |||||||||
193 | 192 | Anna | 08-20 | 418C8 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O60-4 | pSBX1T3-BBa_I13507 | 60 | Tet | #N/A | |||||||||
194 | 193 | Anna | 08-20 | 418C9 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O60-4 | pSBX1T3-BBa_I13507 | 60 | Tet | #N/A | |||||||||
195 | 194 | Anna | 08-20 | 418C10 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O60-4 | pSBX1T3-BBa_I13507 | 60 | Tet | #N/A | |||||||||
196 | 195 | Anna | 08-20 | 417C3 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O60-3 | pSBX1T3-BBa_I13507 | 60 | Tet | der einzige der richtig funktioniert | #N/A | FG1180 | ok | ||||||
197 | 196 | MaxW | 08-21 | 476C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G110#2 | O110-2 | pSBX1K3-2Zv2Npu:(LambdaLys-H6) | 110 | Kan | taugt nix | 76DB28 | several deletions | #N/A | #N/A | ||||
198 | 197 | MaxW | 08-21 | 476C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G110#2 | O110-3 | pSBX1K3-2Zv2Npu:(LambdaLys-H6) | 110 | Kan | 76DB77, 76DB82 | a deletion, a deletion | #N/A | #N/A | |||||
199 | 198 | MaxW | 08-21 | 476C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G110#2 | pSBX1K3-2Zv2Npu:(LambdaLys-H6) | 110 | Kan | #N/A | #N/A | #N/A | |||||||
200 | 199 | Lisa | 08-22 | 485C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A122 | O122 | pSBX1K3-1GateNpuDnaE(N) | 122 | Kan | #N/A | #N/A | |||||||
201 | 200 | Lisa | 08-23 | 486C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A123 | O123 | pSBX1K3-1GateNpuDnaE(C) | 123 | Kan | II2290, II2291 | mutation A220G K74E in the mRFP CDS, besides ok, mutation A220G K74E in the mRFP CDS, besides ok | #N/A | #N/A | |||||
202 | 201 | Lisa | 08-24 | 486C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A123 | O123-2 | pSBX1K3-1GateNpuDnaE(C) | 123 | Kan | #N/A | #N/A | |||||||
203 | 202 | MaxW | 8/23/2014 | 501C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G111#2 | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) | 111 | Kan | #N/A | #N/A | #N/A | |||||||
204 | 203 | MaxW | 8/23/2014 | 501C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G111#2 | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) | 111 | Kan | #N/A | #N/A | #N/A | |||||||
205 | 204 | MaxW | 8/23/2014 | 501C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G111#2 | O111-2 | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) | 111 | Kan | II2294, II2295 | EINE, 1, EINE fucking mutation........., EINE, 1, EINE fucking mutation......... | #N/A | #N/A | |||||
206 | 205 | MaxW | 8/23/2014 | 503C5 | 25% glycerol in LB | 3 | E. coli | Turbos | G113#2 | O113-2 | pSBX1K3-2Zv2Npu:Xyla_circ_noHis | 113 | Kan | II2298, II2299 | fürn arsch, fürn arsch | #N/A | #N/A | |||||
207 | 206 | MaxW | 8/23/2014 | 502C6 | 25% glycerol in LB | 3 | E. coli | Turbos | G112#2 | O112-2 | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His | 112 | Kan | II2296, II2297 | ok, ok | #N/A | #N/A | |||||
208 | 207 | MaxW | 8/23/2014 | 502C7 | 25% glycerol in LB | 3 | E. coli | Turbos | G112#2 | O112-3 | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His | 112 | Kan | discard | #N/A | #N/A | ||||||
209 | 208 | MaxW | 8/23/2014 | 502C8 | 25% glycerol in LB | 3 | E. coli | Turbos | G112#2 | O112-4 | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His | 112 | Kan | discard | #N/A | #N/A | ||||||
210 | 209 | MaxW | 8/23/2014 | 504C6 | 25% glycerol in LB | 3 | E. coli | Turbos | G124 | O124 | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext | 124 | Kan | 76DB73, 76DB78 | insertions, insertions | #N/A | #N/A | |||||
211 | 210 | MaxW | 8/23/2014 | 504C7 | 25% glycerol in LB | 3 | E. coli | Turbos | G124 | O124-2 | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext | 124 | Kan | II2300, II2301 | fürn arsch, fürn arsch | #N/A | #N/A | |||||
212 | 211 | MaxW | 8/23/2014 | 504C8 | 25% glycerol in LB | 3 | E. coli | Turbos | G124 | O124-3 | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext | 124 | Kan | 76DB74, 76DB79 | ok, ok | #N/A | #N/A | |||||
213 | 212 | MaxW | 8/23/2014 | 504C9 | 25% glycerol in LB | 3 | E. coli | Turbos | G124 | O124-4 | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext | 124 | Kan | #N/A | #N/A | |||||||
214 | 213 | MaxW | 8/23/2014 | 512C5 | 25% glycerol in LB | 3 | E. coli | Turbos | L125 | O125 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB29, 76DB30 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
215 | 214 | MaxW | 8/23/2014 | 512C6 | 25% glycerol in LB | 3 | E. coli | Turbos | L125 | O125-2 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB31, 76DB32 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
216 | 215 | MaxW | 8/23/2014 | 512C7 | 25% glycerol in LB | 3 | E. coli | Turbos | L125 | O125-3 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB33, 76DB34 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
217 | 216 | Charlotte | 8/24/2014 | 500C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | S86#5 | pSB1AK8-BBa_I712074-2ZSspH6:GFP | 86 | Amp, Kan | #N/A | #N/A | #N/A | |||||||
218 | 217 | Charlotte | 8/24/2014 | 506C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | S86#3 | pSB1AK8-BBa_I712074-2ZSspH6:GFP | 86 | Amp, Kan | #N/A | #N/A | #N/A | |||||||
219 | 218 | Charlotte | 8/24/2014 | 510C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | S86#5 | O86-3 | pSB1AK8-BBa_I712074-2ZSspH6:GFP | 86 | Amp, Kan | II2310, II2311 | ok, ok | #N/A | #N/A | |||||
220 | 219 | Caro | 8/24/2014 | 527C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A105 | O105 | pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 | 105 | Kan | discard | #N/A | #N/A | ||||||
221 | 220 | Caro | 8/24/2014 | 527C4 | 25% glycerol in LB | 3 | E. coli | Turbos | A105 | O105-2 | pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 | 105 | Kan | II2312, II2313 | ok, ok, C->T in VF2 site | #N/A | #N/A | |||||
222 | 221 | Caro | 8/24/2014 | 528C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A106 | O106 | pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 | 106 | Kan | 76DC22 | ok | #N/A | #N/A | |||||
223 | 222 | Caro | 8/24/2014 | 528C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A106 | O106-2 | pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 | 106 | Kan | II2314, II2315 | mixture A64->T mutation, mixture A64->Tmutation | #N/A | #N/A | |||||
224 | 223 | Caro | 8/24/2014 | 529C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A107 | O107 | pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 | 107 | Kan | II2316, II2317 | ok, good, ok, C->T in VF2 site | #N/A | #N/A | |||||
225 | 224 | Caro | 8/24/2014 | 529C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A107 | O107-2 | pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 | 107 | Kan | discard | #N/A | #N/A | ||||||
226 | 225 | Caro | 8/24/2014 | 530C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A108 | O108 | pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 | 108 | Kan | discard | II2318, II2319 | 1bp deletion before final H6, 1bp deletion in suffix, 1bp deletion before final H6, 1bp deletion in suffix | #N/A | #N/A | ||||
227 | 226 | Caro | 8/24/2014 | 530C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A108 | O108-2 | pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 | 108 | Kan | 76DC11, 76DC12 | mixture with T7RBS T6del, mixture with T7RBS T6del | #N/A | #N/A | |||||
228 | 227 | Silvan | 08-27 | 544C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L125#2 | O125-4 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB51, 76DB57 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
229 | 228 | Silvan | 08-27 | 544C2 | 25% glycerol in LB | 3 | E. coli | Turbos | L125#2 | O125-5 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB52, 76DB58 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
230 | 229 | Silvan | 08-27 | 544C3 | 25% glycerol in LB | 3 | E. coli | Turbos | L125#2 | O125-6 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB53, 76DB59 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
231 | 230 | Silvan | 08-27 | 544C4 | 25% glycerol in LB | 3 | E. coli | Turbos | L125#2 | O125-7 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB54, 76DB60 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
232 | 231 | Silvan | 08-27 | 544C5 | 25% glycerol in LB | 3 | E. coli | Turbos | L125#2 | O125-8 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB55, 76DB61 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
233 | 232 | Silvan | 08-27 | 544C6 | 25% glycerol in LB | 3 | E. coli | Turbos | L125#2 | O125-9 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 76DB56, 76DB62 | negative, contains GFP, negative, contains GFP | #N/A | #N/A | |||||
234 | 233 | Jakob | 08-27 | 525C1-1 | 25% glycerol in LB | 3 | E. coli | Turbos | S94#4 | O94-9 | pSBX4C5-2GateNpuDnaE(N) | 94 | Cm | #N/A | #N/A | |||||||
235 | 234 | Jakob | 08-27 | 525C2-1 | 25% glycerol in LB | 3 | E. coli | Turbos | S94#4 | O94-10 | pSBX4C5-2GateNpuDnaE(N) | 94 | Cm | #N/A | #N/A | |||||||
236 | 235 | Jakob | 08-27 | 526C1-1 | 25% glycerol in LB | 3 | E. coli | Turbos | S95#4 | O95-9 | pSBX4C5-2GateNpuDnaE(C) | 95 | Cm | #N/A | #N/A | |||||||
237 | 236 | Jakob | 08-27 | 526C2-1 | 25% glycerol in LB | 3 | E. coli | Turbos | S95#4 | O95-10 | pSBX4C5-2GateNpuDnaE(C) | 95 | Cm | #N/A | #N/A | |||||||
238 | 237 | MaxW | 08-28 | 543C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G131 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | #N/A | #N/A | #N/A | |||||||
239 | 238 | MaxW | 08-28 | 543C5 | 25% glycerol in LB | 3 | E. coli | Turbos | G131 | O131 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | 76DB75, 76DB80 | gfp..............................., gfp............................... | #N/A | #N/A | |||||
240 | 239 | MaxW | 08-28 | 543C7 | 25% glycerol in LB | 3 | E. coli | Turbos | G131 | O131-2 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | 76DB76, 76DB81 | ccdb missing, ccdb missing | #N/A | #N/A | |||||
241 | 240 | Lisa | 08-28 | 547C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A132 | O132 | pSB1C3-Plac-GFP-IntN-MTS | 132 | Cm | discard | 76DB83, 76DB84 | is actually psbx ssp h6 gfp, is actually psbx ssp h6 gfp | #N/A | #N/A | ||||
242 | 241 | Lisa | 08-28 | 547C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A132 | O132-2 | pSB1C3-Plac-GFP-IntN-MTS | 132 | Cm | discard | #N/A | #N/A | ||||||
243 | 242 | Lisa | 08-28 | 547C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A132 | O132-3 | pSB1C3-Plac-GFP-IntN-MTS | 132 | Cm | discard | #N/A | #N/A | ||||||
244 | 243 | Caro | 08-28 | 552C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | S129#3 | O129-2 | pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC | 129 | #N/A | #N/A | #N/A | |||||||
245 | 244 | Caro | 08-28 | 552C3 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | S129#3 | O129-3 | pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC | 129 | #N/A | #N/A | #N/A | |||||||
246 | 245 | Consti | 08-30 | 562C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G113#3 | O113-3 | pSBX1K3-2Zv2Npu:Xyla_circ_noHis | 113 | Kan | 76DC23, 76DC24 | ok, ok | #N/A | #N/A | |||||
247 | 246 | Consti | 08-30 | 562C4 | 25% glycerol in LB | 3 | E. coli | Turbos | G113#3 | O113-4 | pSBX1K3-2Zv2Npu:Xyla_circ_noHis | 113 | Kan | 76DB87, 76DB88 | ok, ok | #N/A | #N/A | |||||
248 | 247 | Consti | 08-30 | 563C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O112-2 | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His | 112 | Kan | #N/A | II2296, II2297 | ok, ok | |||||||
249 | 248 | Consti | 08-30 | 563C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O112-2 | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His | 112 | Kan | #N/A | II2296, II2297 | ok, ok | |||||||
250 | 249 | MaxW | 09-01 | 583C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L131#2 | O131-3 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | red | #N/A | #N/A | ||||||
251 | 250 | MaxW | 09-01 | 583C2 | 25% glycerol in LB | 3 | E. coli | Turbos | L131#2 | O131-4 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | 76DC01, 76DC02 | ok, ok | #N/A | #N/A | |||||
252 | 251 | MaxW | 09-01 | 583C4 | 25% glycerol in LB | 3 | E. coli | Turbos | L131#2 | O131-5 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | #N/A | #N/A | |||||||
253 | 252 | MaxW | 09-01 | 583C6 | 25% glycerol in LB | 3 | E. coli | Turbos | L131#2 | O131-6 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | #N/A | #N/A | |||||||
254 | 253 | MaxW | 09-01 | 584C1 | 25% glycerol in LB | 3 | E. coli | Turbos | L141 | O141 | pSBX1K3-2Zv2Npu:LaLys+ccdb | 141 | Kan | #N/A | #N/A | |||||||
255 | 254 | MaxW | 09-01 | 584C3 | 25% glycerol in LB | 3 | E. coli | Turbos | L141 | O141-2 | pSBX1K3-2Zv2Npu:LaLys+ccdb | 141 | Kan | 76DC03, 76DC04 | ok, ok | #N/A | #N/A | |||||
256 | 255 | MaxW | 09-01 | 584C5 | 25% glycerol in LB | 3 | E. coli | Turbos | L141 | O141-3 | pSBX1K3-2Zv2Npu:LaLys+ccdb | 141 | Kan | #N/A | #N/A | |||||||
257 | 256 | MaxW | 09-01 | 585C4 | 25% glycerol in LB | 3 | E. coli | Turbos | G131#2 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | red | #N/A | #N/A | #N/A | ||||||
258 | 257 | MaxW | 09-01 | 585C5 | 25% glycerol in LB | 3 | E. coli | Turbos | G131#2 | O131-7 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | 76DC05, 76DC06 | ok, ok | #N/A | #N/A | |||||
259 | 258 | MaxW | 09-01 | 585C6 | 25% glycerol in LB | 3 | E. coli | Turbos | G131#2 | O131-8 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | 76DC07, 76DC08 | ok, ok | #N/A | #N/A | |||||
260 | 259 | MaxW | 09-01 | 585C7 | 25% glycerol in LB | 3 | E. coli | Turbos | G131#2 | O131-9 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | #N/A | #N/A | |||||||
261 | 260 | MaxW | 09-01 | 585C9 | 25% glycerol in LB | 3 | E. coli | Turbos | G131#2 | O131-10 | pSBX4C5-2Zv2Npu:LaLys+ccdb | 131 | Cm | #N/A | #N/A | |||||||
262 | 261 | MaxW | 09-01 | 586C5 | 25% glycerol in LB | 3 | E. coli | Turbos | G141 | O141-4 | pSBX1K3-2Zv2Npu:LaLys+ccdb | 141 | Kan | #N/A | #N/A | |||||||
263 | 262 | MaxW | 09-01 | 586C8 | 25% glycerol in LB | 3 | E. coli | Turbos | G141 | O141-5 | pSBX1K3-2Zv2Npu:LaLys+ccdb | 141 | Kan | #N/A | #N/A | |||||||
264 | 263 | MaxW | 09-01 | 586C9 | 25% glycerol in LB | 3 | E. coli | Turbos | G141 | O141-6 | pSBX1K3-2Zv2Npu:LaLys+ccdb | 141 | Kan | 76DC09, 76DC10 | mutation in ccdb (R->H), mutation in ccdb (R->H) | #N/A | #N/A | |||||
265 | 264 | Jakob | 09-01 | 564C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G137 | O137-4 | pSBX4C5-1Gate-NpuDnaE(N)-H6 | 137 | Cm | 76DC17, 76DC18 | ok, ok | #N/A | #N/A | |||||
266 | 265 | Jakob | 09-01 | 565C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G138 | O138-4 | pSBX4C5-H6-1GateNpuDnaE(C) | 138 | Cm | 76DC20, 76DC21 | ok, ok | #N/A | #N/A | |||||
267 | 266 | Anna | 09-01 | 595C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S133 | O133 | pSB1AK8-BBa_I712074-BBa_I13507 | 133 | Amp, Kan | #N/A | #N/A | |||||||
268 | 267 | Anna | 09-01 | 595C2 | 25% glycerol in LB | 3 | E. coli | Turbos | S133 | O133-3 | pSB1AK8-BBa_I712074-BBa_I13507 | 133 | Amp, Kan | #N/A | #N/A | |||||||
269 | 268 | Anna | 09-01 | 595C3 | 25% glycerol in LB | 3 | E. coli | Turbos | S133 | O133-4 | pSB1AK8-BBa_I712074-BBa_I13507 | 133 | Amp, Kan | #N/A | #N/A | |||||||
270 | 269 | Anna | 09-01 | 573C4 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O57-5 | pSBX1A3-BBa_I13507 | 57 | Amp | #N/A | |||||||||
271 | 270 | Anna | 09-01 | 573C5 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O57-5 | pSBX1A3-BBa_I13507 | 57 | Amp | #N/A | |||||||||
272 | 271 | Anna | 09-01 | 573C6 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O57-5 | pSBX1A3-BBa_I13507 | 57 | Amp | #N/A | |||||||||
273 | 272 | Anna | 09-01 | 573C7 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O57-5 | pSBX1A3-BBa_I13507 | 57 | Amp | #N/A | |||||||||
274 | 273 | Lisa | 09-02 | 567C2 | 25% glycerol in LB | 3 | E. coli | Turbos | S140 | O140 | pSB1C3-T7RBS_SspDnaBC_Ext | 140 | Cm | 76DB99, 76DC00 | it has a his tag (the two plates were mixed up) -> juhu! intein protease ready, | #N/A | #N/A | |||||
275 | 274 | Lisa | 09-02 | 567C3 | 25% glycerol in LB | 3 | E. coli | Turbos | S140 | O140-2 | pSB1C3-T7RBS_SspDnaBC_Ext | 140 | Cm | #N/A | #N/A | |||||||
276 | 275 | Caro | 09-02 | 601C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | S130#5 | O130 | pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG | 130 | #N/A | BI2119, BI2120 | , | #N/A | #N/A | |||||
277 | 276 | Caro | 09-02 | 602C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S130#5 | O130-2 | pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG | 130 | #N/A | #N/A | #N/A | |||||||
278 | 277 | MaxW | 09-02 | 596C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O111-3 | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) | 111 | Kan | #N/A | 76BD93, 76BD94 | ok, ok | |||||||
279 | 278 | MaxW | 09-02 | 596C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O111-3 | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) | 111 | Kan | #N/A | 76BD93, 76BD94 | ok, ok | |||||||
280 | 279 | MaxW | 09-02 | 597C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O110-4 | pSBX1K3-2Zv2Npu:(LambdaLys-H6) | 110 | Kan | #N/A | 76BD97, 76BD98 | ok, ok | |||||||
281 | 280 | MaxW | 09-02 | 597C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O110-4 | pSBX1K3-2Zv2Npu:(LambdaLys-H6) | 110 | Kan | #N/A | 76BD97, 76BD98 | ok, ok | |||||||
282 | 281 | MaxW | 09-02 | 598C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O124-3 | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext | 124 | Kan | #N/A | 76DB74, 76DB79 | ok, ok | |||||||
283 | 282 | MaxW | 09-02 | 598C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O124-3 | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext | 124 | Kan | #N/A | 76DB74, 76DB79 | ok, ok | |||||||
284 | 283 | MaxW | 09-04 | 610C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A160 | O160 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 160 | Kan | BI2115, BI2116 | mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R), mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R) | #N/A | #N/A | |||||
285 | 284 | MaxW | 09-04 | 610C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A160 | O160-2 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 160 | Kan | BI2117, BI2118 | bad sequencing, auch eine in mrfp | #N/A | #N/A | |||||
286 | 285 | MaxW | 09-04 | 610C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A160 | O160-3 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 160 | Kan | BI2147, BI2148 | insertion in smt3, silent in mfrp | #N/A | #N/A | |||||
287 | 286 | MaxW | 09-04 | 610C4 | 25% glycerol in LB | 3 | E. coli | Turbos | A160 | O160-4 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 160 | Kan | BI2149, BI2150, AF9230 | ok, ok (silent in mrfp), | #N/A | #N/A | |||||
288 | 287 | MaxW | 09-04 | 611C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O113-3 | pSBX1K3-2Zv2Npu:Xyla_circ_noHis | 113 | Kan | #N/A | 76DC23, 76DC24 | ok, ok | |||||||
289 | 288 | MaxW | 09-04 | 611C2 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O113-3 | pSBX1K3-2Zv2Npu:Xyla_circ_noHis | 113 | Kan | #N/A | 76DC23, 76DC24 | ok, ok | |||||||
290 | 289 | Lisa | 09-04 | 609C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S159 | O159 | pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext | 159 | Cm | #N/A | #N/A | |||||||
291 | 290 | Lisa | 09-04 | 609C3 | 25% glycerol in LB | 3 | E. coli | Turbos | S159 | O159-2 | pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext | 159 | Cm | #N/A | #N/A | |||||||
292 | 291 | Lisa | 09-04 | 609C5 | 25% glycerol in LB | 3 | E. coli | Turbos | S159 | O159-3 | pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext | 159 | Cm | 76DB85, 76DB86 | ok, ok, juhu, one part of intein protease ready, you should ring bell | #N/A | #N/A | |||||
293 | 292 | Caro | 9/4/2014 | 612C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G145 | O145 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 | 145 | Cm | BI2121, BI2122 | , | #N/A | #N/A | |||||
294 | 293 | Caro | 09-04 | 612C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G145 | O145-2 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 | 145 | Cm | #N/A | #N/A | |||||||
295 | 294 | Caro | 9/4/2014 | 613C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G146 | O146 | pSBX4C5-H6-NpuC-mRFP155-225-H6 | 146 | Cm | BI2123, BI2124 | , | #N/A | #N/A | |||||
296 | 295 | Caro | 09-04 | 613C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G146 | O146-2 | pSBX4C5-H6-NpuC-mRFP155-225-H6 | 146 | Cm | #N/A | #N/A | |||||||
297 | 296 | Caro | 9/4/2014 | 614C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G147 | O147 | pSBX4C5-H6-mRFP1-168-NpuN-H6 | 147 | Cm | BI2125, BI2126 | , | #N/A | #N/A | |||||
298 | 297 | Caro | 09-04 | 614C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G147 | O147-2 | pSBX4C5-H6-mRFP1-168-NpuN-H6 | 147 | Cm | #N/A | #N/A | |||||||
299 | 298 | Caro | 9/4/2014 | 615C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G148 | O148 | pSBX4C5-H6-NpuC-mRFP169-225-H6 | 148 | Cm | BI2127, BI2128 | , | #N/A | #N/A | |||||
300 | 299 | Caro | 09-04 | 615C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G148 | O148-2 | pSBX4C5-H6-NpuC-mRFP169-225-H6 | 148 | Cm | #N/A | #N/A | |||||||
301 | 300 | Caro | 9/4/2014 | 616C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G149 | O149 | pSBX4C5-H6-RenLucN-NpuN-H6 | 149 | Cm | BI2129, BI2130 | , | #N/A | #N/A | |||||
302 | 301 | Caro | 09-04 | 616C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G149 | O149-2 | pSBX4C5-H6-RenLucN-NpuN-H6 | 149 | Cm | #N/A | #N/A | |||||||
303 | 302 | Caro | 9/4/2014 | 617C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G150 | O150 | pSBX4C5-H6-NpuC-RenLucC-H6 | 150 | Cm | BI2131, BI2132 | , | #N/A | #N/A | |||||
304 | 303 | Caro | 09-04 | 617C4 | 25% glycerol in LB | 3 | E. coli | Turbos | G150 | O150-2 | pSBX4C5-H6-NpuC-RenLucC-H6 | 150 | Cm | #N/A | #N/A | |||||||
305 | 304 | Caro | 9/4/2014 | 618C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G151 | O151 | pSBX4C5-H6-FireLucN-NpuN-H6 | 151 | Cm | BI2133, BI2134 | , | #N/A | #N/A | |||||
306 | 305 | Caro | 09-04 | 618C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G151 | O151-2 | pSBX4C5-H6-FireLucN-NpuN-H6 | 151 | Cm | #N/A | #N/A | |||||||
307 | 306 | Caro | 9/4/2014 | 619C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G152 | O152 | pSBX4C5-H6-NpuC-FireLucC-H6 | 152 | Cm | BI2135, BI2136 | , | #N/A | #N/A | |||||
308 | 307 | Caro | 09-04 | 619C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G152 | O152-2 | pSBX4C5-H6-NpuC-FireLucC-H6 | 152 | Cm | #N/A | #N/A | |||||||
309 | 308 | Caro | 9/4/2014 | 620C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G153 | O153 | pSBX4C5-H6-mCherryN-NpuN-H6 | 153 | Cm | BI2137, BI2138 | , | #N/A | #N/A | |||||
310 | 309 | Caro | 09-04 | 620C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G153 | O153-2 | pSBX4C5-H6-mCherryN-NpuN-H6 | 153 | Cm | #N/A | #N/A | |||||||
311 | 310 | Caro | 9/4/2014 | 621C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G154 | O154 | pSBX4C5-H6-NpuC-mCherryC-H6 | 154 | Cm | BI2139, BI2140 | , | #N/A | #N/A | |||||
312 | 311 | Caro | 09-04 | 621C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G154 | O154-2 | pSBX4C5-H6-NpuC-mCherryC-H6 | 154 | Cm | #N/A | #N/A | |||||||
313 | 312 | Caro | 9/4/2014 | 622C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G155 | O155 | pSBX4C5-H6-GFPn1-64-NpuN-H6 | 155 | Cm | BI2141, BI2142 | , | #N/A | #N/A | |||||
314 | 313 | Caro | 09-04 | 622C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G155 | O155-2 | pSBX4C5-H6-GFPn1-64-NpuN-H6 | 155 | Cm | #N/A | #N/A | |||||||
315 | 314 | Caro | 9/4/2014 | 624C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G157 | O157 | pSBX4C5-H6-GFPn1-157-NpuN-H6 | 157 | Cm | BI2143, BI2144 | , | #N/A | #N/A | |||||
316 | 315 | Caro | 09-04 | 624C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G157 | O157 | pSBX4C5-H6-GFPn1-157-NpuN-H6 | 157 | Cm | BI2143, BI2144 | , | #N/A | #N/A | |||||
317 | 316 | Caro | 9/4/2014 | 625C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G158 | O158 | pSBX4C5-H6-GFPc158-238-NpuC-H6 | 158 | Cm | #N/A | #N/A | |||||||
318 | 317 | Caro | 09-04 | 625C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G158 | O158-2 | pSBX4C5-H6-GFPc158-238-NpuC-H6 | 158 | Cm | #N/A | #N/A | |||||||
319 | 318 | CARO | 9/7/2014 | 660C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O105-2 | pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 | 105 | Kan | #N/A | II2312, II2313 | ok, ok, C->T in VF2 site | |||||||
320 | 319 | CARO | 9/7/2014 | 661C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O106 | pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 | 106 | Kan | #N/A | 76DC22 | ok | |||||||
321 | 320 | CARO | 9/7/2014 | 662C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O107 | pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 | 107 | Kan | #N/A | II2316, II2317 | ok, good, ok, C->T in VF2 site | |||||||
322 | 321 | CARO | 9/7/2014 | 663C1 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O108-2 | pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 | 108 | Kan | #N/A | 76DC11, 76DC12 | mixture with T7RBS T6del, mixture with T7RBS T6del | |||||||
323 | 322 | 25% glycerol in LB | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
324 | 323 | MaxW | 09-08 | 646C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A163 | O163 | pSBX4C5-2GateSspDnaX-S11C | 163 | Cm | BI2145, BI2171 | ok, ok | #N/A | #N/A | |||||
325 | 324 | MaxW | 09-08 | 646C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A163 | O163-2 | pSBX4C5-2GateSspDnaX-S11C | 163 | Cm | #N/A | #N/A | |||||||
326 | 325 | MaxW | 09-08 | 646C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A163 | pSBX4C5-2GateSspDnaX-S11C | 163 | Cm | #N/A | #N/A | #N/A | |||||||
327 | 326 | MaxW | 09-08 | 647C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A164 | O164 | pSBX4C5-2GateSspDnaX-S11C HN->AG | 164 | Cm | BI2172, BI2173 | ok, ok | #N/A | #N/A | |||||
328 | 327 | MaxW | 09-08 | 647C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A164 | O164-2 | pSBX4C5-2GateSspDnaX-S11C HN->AG | 164 | Cm | #N/A | #N/A | |||||||
329 | 328 | MaxW | 09-08 | 647C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A164 | pSBX4C5-2GateSspDnaX-S11C HN->AG | 164 | Cm | #N/A | #N/A | #N/A | |||||||
330 | 329 | MaxW | 09-08 | 648C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A165 | O165 | pSBX4C5-2GateSspDnaX-S11N | 165 | Cm | BI2174, BI2175, BI1917 | part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, ok | #N/A | #N/A | |||||
331 | 330 | MaxW | 09-08 | 648C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A165 | O165-2 | pSBX4C5-2GateSspDnaX-S11N | 165 | Cm | #N/A | #N/A | |||||||
332 | 331 | MaxW | 09-08 | 648C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A165 | pSBX4C5-2GateSspDnaX-S11N | 165 | Cm | #N/A | #N/A | #N/A | |||||||
333 | 332 | MaxW | 09-08 | 649C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A166 | O166 | pSBX4C5-2GateSspDnaX-S11N C->G | 166 | Cm | BI2176, BI2177, BI1918 | part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, ok | #N/A | #N/A | |||||
334 | 333 | MaxW | 09-08 | 649C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A166 | O166-2 | pSBX4C5-2GateSspDnaX-S11N C->G | 166 | Cm | #N/A | #N/A | |||||||
335 | 334 | MaxW | 09-08 | 649C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A166 | pSBX4C5-2GateSspDnaX-S11N C->G | 166 | Cm | #N/A | #N/A | #N/A | |||||||
336 | 335 | MaxW | 09-08 | 650C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A167 | O167 | pSBX4C5-2GateSspGyrB-S11C | 167 | Cm | BI2178, BI2179 | ok, ok | #N/A | #N/A | |||||
337 | 336 | MaxW | 09-08 | 650C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A167 | O167-2 | pSBX4C5-2GateSspGyrB-S11C | 167 | Cm | #N/A | #N/A | |||||||
338 | 337 | MaxW | 09-08 | 650C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A167 | pSBX4C5-2GateSspGyrB-S11C | 167 | Cm | #N/A | #N/A | #N/A | |||||||
339 | 338 | MaxW | 09-08 | 651C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A168 | O168 | pSBX4C5-2GateSspGyrB-S11C SN->AG | 168 | Cm | BI2180, BI2181 | ok, ok | #N/A | #N/A | |||||
340 | 339 | MaxW | 09-08 | 651C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A168 | O168-2 | pSBX4C5-2GateSspGyrB-S11C SN->AG | 168 | Cm | #N/A | #N/A | |||||||
341 | 340 | MaxW | 09-08 | 651C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A168 | pSBX4C5-2GateSspGyrB-S11C SN->AG | 168 | Cm | #N/A | #N/A | #N/A | |||||||
342 | 341 | MaxW | 09-08 | 652C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A169 | O169 | pSBX4C5-2GateSspGyrB-S11N | 169 | Cm | BI2182, BI2183, BI1919 | part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, mutation in ccdb c->a (?), ok, only intein sequenzed | #N/A | #N/A | |||||
343 | 342 | MaxW | 09-08 | 652C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A169 | O169-2 | pSBX4C5-2GateSspGyrB-S11N | 169 | Cm | BI2198, BI2199 | ok, ok (mut in ccdb E87*) | #N/A | #N/A | |||||
344 | 343 | MaxW | 09-08 | 652C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A169 | pSBX4C5-2GateSspGyrB-S11N | 169 | Cm | #N/A | #N/A | #N/A | |||||||
345 | 344 | MaxW | 09-08 | 654C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A171 | O171 | pSBX4C5-2GateTerDnaE3-S11C | 171 | Cm | BI2184, BI2185 | ok, ok | #N/A | #N/A | |||||
346 | 345 | MaxW | 09-08 | 654C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A171 | O171-2 | pSBX4C5-2GateTerDnaE3-S11C | 171 | Cm | #N/A | #N/A | |||||||
347 | 346 | MaxW | 09-08 | 654C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A171 | pSBX4C5-2GateTerDnaE3-S11C | 171 | Cm | #N/A | #N/A | #N/A | |||||||
348 | 347 | MaxW | 09-08 | 655C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A172 | O172 | pSBX4C5-2GateTerDnaE3-S11C SN->AG | 172 | Cm | BI2186, BI2187 | ok, ok | #N/A | #N/A | |||||
349 | 348 | MaxW | 09-08 | 655C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A172 | O172-2 | pSBX4C5-2GateTerDnaE3-S11C SN->AG | 172 | Cm | #N/A | #N/A | |||||||
350 | 349 | MaxW | 09-08 | 656C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A173 | O173 | pSBX4C5-2GateTerDnaE3-S11N | 173 | Cm | BI2188, BI2189 | mutation in lacO-reg g->t, part of mrfp+intein not sequenced, part of mrfp+intein not sequenced | #N/A | #N/A | |||||
351 | 350 | MaxW | 09-08 | 656C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A173 | O173-2 | pSBX4C5-2GateTerDnaE3-S11N | 173 | Cm | BI2200, BI2201, BI1921 | ok, ok, ok | #N/A | #N/A | |||||
352 | 351 | MaxW | 09-08 | 656C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A173 | pSBX4C5-2GateTerDnaE3-S11N | 173 | Cm | #N/A | #N/A | #N/A | |||||||
353 | 352 | MaxW | 09-08 | 657C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A174 | O174 | pSBX4C5-2GateTerDnaE3-S11N C->G | 174 | Cm | BI2190, BI2191 | no mutations, begins in ccdb --> not fully seq, mut. in ccdb ga->tc | #N/A | #N/A | |||||
354 | 353 | MaxW | 09-08 | 657C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A174 | O174-2 | pSBX4C5-2GateTerDnaE3-S11N C->G | 174 | Cm | BI2202, BI2203, BI1922 | ok, ok, ok | #N/A | #N/A | |||||
355 | 354 | MaxW | 09-08 | 657C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A174 | pSBX4C5-2GateTerDnaE3-S11N C->G | 174 | Cm | #N/A | #N/A | #N/A | |||||||
356 | 355 | MaxW | 09-08 | 658C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A143 | O143 | pSBX4C5-2GateNpuDnaE(N)* | 143 | Cm | BI2192, BI2193 | part of intein/mrfp not sequenced, part of intein/mrfp not sequenced | #N/A | #N/A | |||||
357 | 356 | MaxW | 09-08 | 658C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A143 | O143-2 | pSBX4C5-2GateNpuDnaE(N)* | 143 | Cm | BI2204, BI2205, BI1923 | ok, ok, ok | #N/A | #N/A | |||||
358 | 357 | MaxW | 09-08 | 658C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A143 | pSBX4C5-2GateNpuDnaE(N)* | 143 | Cm | #N/A | #N/A | #N/A | |||||||
359 | 358 | MaxW | 09-08 | 659C1 | 25% glycerol in LB | 3 | E. coli | Turbos | A144 | O144 | pSBX4C5-2GateNpuDnaE(C)* | 144 | Cm | #N/A | #N/A | |||||||
360 | 359 | MaxW | 09-08 | 659C2 | 25% glycerol in LB | 3 | E. coli | Turbos | A144 | O144-2 | pSBX4C5-2GateNpuDnaE(C)* | 144 | Cm | BI2206, BI2207 | ok, ok | #N/A | #N/A | |||||
361 | 360 | MaxW | 09-08 | 659C3 | 25% glycerol in LB | 3 | E. coli | Turbos | A144 | pSBX4C5-2GateNpuDnaE(C)* | 144 | Cm | #N/A | #N/A | #N/A | |||||||
362 | 361 | Caro | 09-08 | 540C4 | 25% glycerol in LB | 6 | E. coli | BI21(DE3) | O128 | pSBX1K3-T7RBS-H6-sfGFP-H6 | 128 | Kan | #N/A | 76DB36, 76DB37 | perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg, perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg | |||||||
363 | 362 | Caro | 09-08 | 667C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O122-3 | pSBX1K3-1GateNpuDnaE(N) | 122 | Kan | #N/A | II2320, II2321 | ok, perfect, ok, perfect | |||||||
364 | 363 | Caro | 09-10 | 668C1 | 25% glycerol in LB | 3 | E. coli | Turbos | O123-5 | pSBX1K3-1GateNpuDnaE(C) | 123 | Kan | #N/A | II2322, II2323 | ok, perfect, ok, perfect | |||||||
365 | 364 | Caro | 09-10 | 688C1 | 25% glycerol in LB | 3 | E. coli | Turbos | G179 | O180 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 180 | Kan | BI1915, BI1916 | deletion in 1st Stop codon, deletion in 1st Stop codon | #N/A | #N/A | |||||
366 | 365 | Caro | 09-10 | 688C2 | 25% glycerol in LB | 3 | E. coli | Turbos | G179 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 180 | Kan | #N/A | #N/A | #N/A | |||||||
367 | 366 | Caro | 09-10 | 688C3 | 25% glycerol in LB | 3 | E. coli | Turbos | G179 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 180 | Kan | #N/A | #N/A | #N/A | |||||||
368 | 367 | MaxW | 09-10 | 684C6 | 25% glycerol in LB | 3 | E. coli | Turbos | A170#2 | O170 | pSBX4C5-2GateSspGyrB-S11N C->G | 170 | Cm | BI2208, BI2209, BI1920 | ok, ok, ok | #N/A | #N/A | |||||
369 | 368 | MaxW | 09-10 | 684C7 | 25% glycerol in LB | 3 | E. coli | Turbos | A170#2 | O170-2 | pSBX4C5-2GateSspGyrB-S11N C->G | 170 | Cm | discard | #N/A | #N/A | ||||||
370 | 369 | MaxW | 09-10 | 684C8 | 25% glycerol in LB | 3 | E. coli | Turbos | A170#2 | O170-3 | pSBX4C5-2GateSspGyrB-S11N C->G | 170 | Cm | discard | #N/A | #N/A | ||||||
371 | 370 | MaxW | 09-10 | 685C6 | 25% glycerol in LB | 3 | E. coli | Turbos | A135b#2 | O135 | pSBX4C5-2GateGp41-1(N) | 135 | Cm | BI2210, BI2211, BI1924 | ok, ok, ok | #N/A | #N/A | |||||
372 | 371 | MaxW | 09-10 | 685C7 | 25% glycerol in LB | 3 | E. coli | Turbos | A135b#2 | O135-2 | pSBX4C5-2GateGp41-1(N) | 135 | Cm | discard | #N/A | #N/A | ||||||
373 | 372 | MaxW | 09-10 | 685C8 | 25% glycerol in LB | 3 | E. coli | Turbos | A135b#2 | O135-3 | pSBX4C5-2GateGp41-1(N) | 135 | Cm | discard | #N/A | #N/A | ||||||
374 | 373 | MaxW | 09-10 | 686C6 | 25% glycerol in LB | 3 | E. coli | Turbos | G179 | O136 | pSBX4C5-2GateGp41-1(C) | 136 | Cm | BI2212, BI2213 | ok, ok | #N/A | #N/A | |||||
375 | 374 | MaxW | 09-10 | 686C7 | 25% glycerol in LB | 3 | E. coli | Turbos | G179 | O136-2 | pSBX4C5-2GateGp41-1(C) | 136 | Cm | discard | #N/A | #N/A | ||||||
376 | 375 | MaxW | 09-10 | 686C8 | 25% glycerol in LB | 3 | E. coli | Turbos | G179 | O136-3 | pSBX4C5-2GateGp41-1(C) | 136 | Cm | discard | #N/A | #N/A | ||||||
377 | 376 | Consti | 09-11 | 692C1 | 25% glycerol in LB | 3 | E. coli | Turbos | S181 | O181 | pSBX1K3-Xylanase | 181 | Kan | BI1947 | ok | #N/A | #N/A | |||||
378 | 377 | Consti | 09-11 | 692C2 | 25% glycerol in LB | 3 | E. coli | Turbos | S181 | O181-2 | pSBX1K3-Xylanase | 181 | Kan | discard | #N/A | #N/A | ||||||
379 | 378 | Consti | 09-11 | 692C3 | 25% glycerol in LB | 3 | E. coli | Turbos | S181 | O181-3 | pSBX1K3-Xylanase | 181 | Kan | BI12214 | ok | #N/A | #N/A | |||||
380 | 379 | Consti | 09-11 | 692C4 | 25% glycerol in LB | 3 | E. coli | Turbos | S181 | O181-4 | pSBX1K3-Xylanase | 181 | Kan | discard | #N/A | #N/A | ||||||
381 | 380 | Consti | 09-11 | 692C5 | 25% glycerol in LB | 3 | E. coli | Turbos | S181 | O181-5 | pSBX1K3-Xylanase | 181 | Kan | discard | #N/A | #N/A | ||||||
382 | 381 | Silvan | 09-11 | 544C8-1 | 25% glycerol in H2O | 3 | E. coli | Turbos | L125#2 | O125-10 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | BI1925, BI1932 | , | #N/A | #N/A | |||||
383 | 382 | Silvan | 09-11 | 676C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A178 | O178 | pSB1C3-H6-0ZSortA | 178 | Cm | BI1929, BI1936, AG3767 | ok, no result, ok | #N/A | #N/A | |||||
384 | 383 | Silvan | 09-11 | 676C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A178 | O178-2 | pSB1C3-H6-0ZSortA | 178 | Cm | BI1930, BI1937, AG3768 | ok, no result, ok | #N/A | #N/A | |||||
385 | 384 | Silvan | 09-11 | 676C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A178 | O178-3 | pSB1C3-H6-0ZSortA | 178 | Cm | BI1931, BI1938, AG3769 | H9R mutation, no result, ok | #N/A | #N/A | |||||
386 | 385 | Silvan | 09-11 | 675C1-1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A177 | O177 | pSB1C3-2ZSortA-H6 | 177 | Cm | BI1926, BI1933 | , | #N/A | #N/A | |||||
387 | 386 | Silvan | 09-11 | 675C2-1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A177 | O177-2 | pSB1C3-2ZSortA-H6 | 177 | Cm | BI1927, BI1934 | , | #N/A | #N/A | |||||
388 | 387 | Silvan | 09-11 | 675C3-1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A177 | O177-3 | pSB1C3-2ZSortA-H6 | 177 | Cm | BI1928, BI1935 | , | #N/A | #N/A | |||||
389 | 388 | MaxW | 09-12 | 705C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G188 | O188 | pSBX1K3-LiLaLy(H6) | 188 | Kan | BI1939, BI1940 | , luciferase?? | #N/A | #N/A | |||||
390 | 389 | MaxW | 09-12 | 705C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G188 | O188-2 | pSBX1K3-LiLaLy(H6) | 188 | Kan | #N/A | #N/A | |||||||
391 | 390 | MaxW | 09-12 | 705C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G188 | O188-3 | pSBX1K3-LiLaLy(H6) | 188 | Kan | BI1941, BI1942 | , luciferase?? | #N/A | #N/A | |||||
392 | 391 | MaxW | 09-12 | 706C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G189 | O189 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 189 | Cm | BI1943, BI1944 | , is pSBX1K3-LiLaLy(H6) | #N/A | #N/A | |||||
393 | 392 | MaxW | 09-12 | 706C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G189 | O189-2 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 189 | Cm | BI1945, BI1946 | , is pSBX1K3-LiLaLy(H6) | #N/A | #N/A | |||||
394 | 393 | MaxW | 09-12 | 706C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G189 | O189-3 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 189 | Cm | #N/A | #N/A | |||||||
395 | 394 | Lisa | 09-12 | 710C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S161#5 | O161 | pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI | 161 | Cm | BI1952 | hä? | #N/A | #N/A | |||||
396 | 395 | Lisa | 09-12 | 710C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S161#5 | O161-2 | pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI | 161 | Cm | #N/A | #N/A | |||||||
397 | 396 | Lisa | 09-12 | 710C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | S161#5 | O161-3 | pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI | 161 | Cm | #N/A | #N/A | |||||||
398 | 397 | Caro | 09-12 | 694C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S182 | O182 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 182 | Cm | BI1948, BI1949 | ok, ok | #N/A | #N/A | |||||
399 | 398 | Caro | 09-12 | 694C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S182 | O182-2 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 182 | Cm | BI1950, BI1951 | ok, ok | #N/A | #N/A | |||||
400 | 399 | Caro | 09-12 | 693C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O180 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 180 | Kan | #N/A | BI1915, BI1916 | deletion in 1st Stop codon, deletion in 1st Stop codon | |||||||
401 | 400 | Caro | 09-13 | 700C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O65-8 | pSB1C3-BBa_J04450 | 65 | Cm | #N/A | |||||||||
402 | 401 | Caro | 09-13 | 701C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O88 | pSB1C3-BBa_J52008 | 88 | Cm | #N/A | FG1194 | ok | |||||||
403 | 402 | Caro | 09-13 | 702C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O89 | pSB1AK8-BBa_I712019 | 89 | Amp, Kan | #N/A | FG1195 | ok, good | |||||||
404 | 403 | Caro | 09-13 | 703C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O90 | pSB1C3-BBa_J06702 | 90 | Cm | #N/A | FG1196 | ok, good | |||||||
405 | 404 | Caro | 09-13 | 704C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O91-2 | pSB1A2-BBa_E0040 | 91 | Amp | #N/A | |||||||||
406 | 405 | Caro | 722C2??? | 25% glycerol in H2O | #N/A | #N/A | #N/A | G201 | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | |||||||
407 | 406 | Caro | 722C3??? | 25% glycerol in H2O | #N/A | #N/A | #N/A | G201 | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | |||||||
408 | 407 | Lisa | 09-15 | 734C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S207 | O207 | pSB1C3-EX-BsaI-CC-O-IasB-S | 207 | Cm | #N/A | #N/A | |||||||
409 | 408 | Lisa | 09-15 | 734C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | S207 | O207-2 | pSB1C3-EX-BsaI-CC-O-IasB-S | 207 | Cm | BI1953, BI1954 | is O208-2......oo, 3.G afterprefix to AC, is O208-2......oo, 3.G afterprefix to AC | #N/A | #N/A | |||||
410 | 409 | Lisa | 09-15 | 735C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S208 | O208 | pSB1C3-EX-BsaI-A-NN-IasB-S | 208 | Cm | #N/A | #N/A | |||||||
411 | 410 | Lisa | 09-15 | 735C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | S208 | O208-2 | pSB1C3-EX-BsaI-A-NN-IasB-S | 208 | Cm | BI1955, BI1956 | is O207-2....:(, 3.G afterprefix to AC, is O207-2....:(, 3.G afterprefix to AC | #N/A | #N/A | |||||
412 | 411 | Caro | 736C1 | 25% glycerol in H2O | O182-3 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 182 | Cm | #N/A | BI1972, BI1973 | ok, ok | |||||||||||
413 | 412 | Caro | 738C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O183 | pSBX4C5-mRFPn1-168-mRFPc169-255_coex | 183 | Cm | #N/A | BI1964, BI1965 | ok, ok | ||||||||
414 | 413 | Caro | 740C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O186 | pSBX4C5mCherryN_mCherryC_coex | 186 | Cm | #N/A | BI1966, BI1967 | ok, ok | ||||||||
415 | 414 | Caro | 744C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O187 | pSBX4C5-GFPn1-157_GFPc158-255_coex | 187 | Cm | #N/A | BI1968, BI1969 | ok, ok | ||||||||
416 | 415 | Caro | 09-15 | 781C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O206 | pSB4C5-GFP_contr | 206 | Cm | #N/A | BI1959, BI1960 | ok, ok | |||||||
417 | 416 | Caro | 09-15 | 784C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O205 | pSB4C5-mCherry_contr | 205 | Cm | #N/A | BI1961, BI1962 | ok, ok | |||||||
418 | 417 | Charlotte | 09-16 | 733C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O189 | pSBX1K3-LiLaLy(H6) | 188 | Kan | #N/A | BI1943, BI1944 | , is pSBX1K3-LiLaLy(H6) | |||||||
419 | 418 | MaxW | 09-17 | 794C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G189#2 | O189-4 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 189 | Cm | BI1974, BI1975 | ok, bad seq | #N/A | #N/A | |||||
420 | 419 | MaxW | 09-17 | 794C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G189#2 | O189-5 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 189 | Cm | BI1976, BI1977 | ok, ok | #N/A | #N/A | |||||
421 | 420 | MaxW | 09-17 | 794C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G189#2 | O189-6 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 189 | Cm | #N/A | #N/A | |||||||
422 | 421 | MaxW | 09-17 | 795C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G216 | O216 | pSBX1K3-XylaH6BB | 216 | Kan | #N/A | #N/A | |||||||
423 | 422 | MaxW | 09-17 | 795C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G216 | O216-2 | pSBX1K3-XylaH6BB | 216 | Kan | BI1978 | ok | #N/A | #N/A | |||||
424 | 423 | MaxW | 09-17 | 795C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G216 | O216-3 | pSBX1K3-XylaH6BB | 216 | Kan | #N/A | #N/A | |||||||
425 | 424 | MaxW | 09-17 | 796C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G217 | O217 | pSBX1K3-XylaH6extBB | 217 | Kan | BI1979, BF8279 | bad seq, bad seq | #N/A | #N/A | |||||
426 | 425 | MaxW | 09-17 | 796C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G217 | O217-2 | pSBX1K3-XylaH6extBB | 217 | Kan | BF8280 | ok | #N/A | #N/A | |||||
427 | 426 | MaxW | 09-17 | 796C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G217 | O217-3 | pSBX1K3-XylaH6extBB | 217 | Kan | #N/A | #N/A | |||||||
428 | 427 | Caro | 09-17 | 768C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G192#2 | O192 | pSBX4C5-1GateNpuDnaE(N)*-H6 | 192 | Cm | to be discarded | BI1980 | looks like pSBX4C5-1GateNpuDnaEC_SN->AG_H6 | #N/A | #N/A | ||||
429 | 428 | Caro | 09-17 | 769C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G193#2 | O193 | pSBX4C5-1GateSspDnaX-S11(C)-H6 | 193 | Cm | BI1982 | ok | #N/A | #N/A | |||||
430 | 429 | Caro | 09-17 | 770C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G194#2 | O194 | pSBX4C5-1GateSspDnaX-S11C HN->AG_H6 | 194 | Cm | BI1984 | ok | #N/A | #N/A | |||||
431 | 430 | Caro | 09-17 | 771C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G195#2 | O195 | pSBX4C5-1GateSspDnaX-S11N_H6 | 195 | Cm | BI1986 | ok | #N/A | #N/A | |||||
432 | 431 | Caro | 09-17 | 772C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G196#2 | O196 | pSBX4C5-1GateSspDnaX-S11N C->G_H6 | 196 | Cm | BI1988 | ok | #N/A | #N/A | |||||
433 | 432 | Caro | 09-17 | 773C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G197#2 | O197 | pSBX4C5-1GateSspGyrB-S11C_H6 | 197 | Cm | BI1990 | ok | #N/A | #N/A | |||||
434 | 433 | Caro | 09-17 | 774C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G198#2 | O198 | pSBX4C5-1GateSspGyrB-S11C SN->AG_H6 | 198 | Cm | BI1992 | ok | #N/A | #N/A | |||||
435 | 434 | Caro | 09-17 | 775C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G199#2 | O199 | pSBX4C5-1GateSspGyrB-S11N_H6 | 199 | Cm | BI1994 | ok | #N/A | #N/A | |||||
436 | 435 | Caro | 09-17 | 776C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G200#2 | O200 | pSBX4C5-1GateSspGyrB-S11N C->G_H6 | 200 | Cm | BI1996 | ok | #N/A | #N/A | |||||
437 | 436 | Caro | 09-17 | 777C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G201#2 | O201 | pSBX4C5-1GateTerDnaE3-S11C_H6 | 201 | Cm | BI1998 | ok | #N/A | #N/A | |||||
438 | 437 | Caro | 09-17 | 778C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G202#2 | O202 | pSBX4C5-1GateTerDnaE3-S11C SN->AG_H6 | 202 | Cm | BI2000 | ok | #N/A | #N/A | |||||
439 | 438 | Caro | 09-17 | 779C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G203#2 | O203 | pSBX4C5-1GateTerDnaE3-S11(N)-H6 | 203 | Cm | BI2002 | ok | #N/A | #N/A | |||||
440 | 439 | Caro | 09-17 | 780C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G204#2 | O204 | pSBX4C5-1GateTerDnaE3-S11(N)*-H6 | 204 | Cm | BI2004 | ok | #N/A | #N/A | |||||
441 | 440 | Caro | 09-17 | 811C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G191#2 | O191 | pSBX4C5-1GateNpuDnaE(C)*-H6 | 191 | Cm | BI2006 | looks like pSBX4C5-1GateNpuDnaEN_C->G_H6 | #N/A | #N/A | |||||
442 | 441 | Caro | 09-17 | 800C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O146-3 | pSBX4C5-H6-NpuC-mRFP155-225-H6 | 146 | Cm | #N/A | BI2152, BI2164 | ok, | |||||||
443 | 442 | Caro | 09-17 | 801C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O147-3 | pSBX4C5-H6-mRFP1-168-NpuN-H6 | 147 | Cm | #N/A | BI2153, BI2165 | kok, | |||||||
444 | 443 | Caro | 09-17 | 802C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O148-3 | pSBX4C5-H6-NpuC-mRFP169-225-H6 | 148 | Cm | #N/A | BI2154 | ok | |||||||
445 | 444 | Caro | 09-17 | 803C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O149-3 | pSBX4C5-H6-RenLucN-NpuN-H6 | 149 | Cm | #N/A | BI2155, BI2166 | ok, one read bad, silent mutation in ligation renlucN to NpuN, | |||||||
446 | 445 | Caro | 09-17 | 804C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O151-3 | pSBX4C5-H6-FireLucN-NpuN-H6 | 151 | Cm | #N/A | BI2157, BI2167 | ok, | |||||||
447 | 446 | Caro | 09-17 | 805C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O152-3 | pSBX4C5-H6-NpuC-FireLucC-H6 | 152 | Cm | #N/A | BI2158 | ok | |||||||
448 | 447 | Caro | 09-17 | 806C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O153-3 | pSBX4C5-H6-mCherryN-NpuN-H6 | 153 | Cm | #N/A | BI2159, BI2168 | ok, | |||||||
449 | 448 | Caro | 09-17 | 807C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O154-3 | pSBX4C5-H6-NpuC-mCherryC-H6 | 154 | Cm | #N/A | 76DB91, 76DB92 | ok, ok | |||||||
450 | 449 | Caro | 09-17 | 808C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O155-3 | pSBX4C5-H6-GFPn1-64-NpuN-H6 | 155 | Cm | #N/A | BI2160, BI2169 | ok, | |||||||
451 | 450 | Caro | 09-17 | 809C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O157-3 | pSBX4C5-H6-GFPn1-157-NpuN-H6 | 157 | Cm | #N/A | BI2161, BI2170 | ok, | |||||||
452 | 451 | Caro | 09-17 | 810C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O158-3 | pSBX4C5-H6-GFPc158-238-NpuC-H6 | 158 | Cm | #N/A | BI2162 | ok, one shift after "stem loop" | |||||||
453 | 452 | Caro | 09-17 | 811C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G191#2 | O191-2 | pSBX4C5-1GateNpuDnaE(C)*-H6 | 191 | Cm | BI2008 | looks like pSBX4C5-1GateNpuDnaEN_C->G_H6 | #N/A | #N/A | |||||
454 | 453 | Caro | 09-18 | 800C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O146-3 | pSBX4C5-H6-NpuC-mRFP155-225-H6 | 146 | Cm | #N/A | BI2152, BI2164 | ok, | |||||||
455 | 454 | Caro | 09-18 | 801C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O147-3 | pSBX4C5-H6-mRFP1-168-NpuN-H6 | 147 | Cm | #N/A | BI2153, BI2165 | kok, | |||||||
456 | 455 | Caro | 09-18 | 802C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O148-3 | pSBX4C5-H6-NpuC-mRFP169-225-H6 | 148 | Cm | #N/A | BI2154 | ok | |||||||
457 | 456 | Caro | 09-18 | 803C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O149-3 | pSBX4C5-H6-RenLucN-NpuN-H6 | 149 | Cm | #N/A | BI2155, BI2166 | ok, one read bad, silent mutation in ligation renlucN to NpuN, | |||||||
458 | 457 | Caro | 09-18 | 804C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O151-3 | pSBX4C5-H6-FireLucN-NpuN-H6 | 151 | Cm | #N/A | BI2157, BI2167 | ok, | |||||||
459 | 458 | Caro | 09-18 | 805C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O152-3 | pSBX4C5-H6-NpuC-FireLucC-H6 | 152 | Cm | #N/A | BI2158 | ok | |||||||
460 | 459 | Caro | 09-18 | 806C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O153-3 | pSBX4C5-H6-mCherryN-NpuN-H6 | 153 | Cm | #N/A | BI2159, BI2168 | ok, | |||||||
461 | 460 | Caro | 09-18 | 807C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O154-3 | pSBX4C5-H6-NpuC-mCherryC-H6 | 154 | Cm | #N/A | 76DB91, 76DB92 | ok, ok | |||||||
462 | 461 | Caro | 09-18 | 808C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O155-3 | pSBX4C5-H6-GFPn1-64-NpuN-H6 | 155 | Cm | #N/A | BI2160, BI2169 | ok, | |||||||
463 | 462 | Caro | 09-18 | 809C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O157-3 | pSBX4C5-H6-GFPn1-157-NpuN-H6 | 157 | Cm | #N/A | BI2161, BI2170 | ok, | |||||||
464 | 463 | Caro | 09-18 | 810C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O158-3 | pSBX4C5-H6-GFPc158-238-NpuC-H6 | 158 | Cm | #N/A | BI2162 | ok, one shift after "stem loop" | |||||||
465 | 464 | Maggie | 09-18 | 819C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G209 | O209 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 209 | Kan | #N/A | #N/A | |||||||
466 | 465 | Maggie | 09-18 | 819C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G209 | O209-2 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 209 | Kan | #N/A | #N/A | |||||||
467 | 466 | Maggie | 09-18 | 819C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G209 | O209-3 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 209 | Kan | #N/A | #N/A | |||||||
468 | 467 | Maggie | 09-18 | 819C4 | 25% glycerol in H2O | 3 | E. coli | Turbos | G209 | O209-4 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 209 | Kan | BI2012, IH6892 | ok, ccdB missing | #N/A | #N/A | |||||
469 | 468 | Maggie | 09-18 | 819C5 | 25% glycerol in H2O | 3 | E. coli | Turbos | G209 | O209-5 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 209 | Kan | #N/A | #N/A | |||||||
470 | 469 | Maggie | 09-18 | 819C6 | 25% glycerol in H2O | 3 | E. coli | Turbos | G209 | O209-6 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 209 | Kan | BI2013, IH6893 | really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector), really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector) | #N/A | #N/A | |||||
471 | 470 | Maggie | 09-18 | 819C7 | 25% glycerol in H2O | 3 | E. coli | Turbos | G209 | O209-7 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 209 | Kan | #N/A | #N/A | |||||||
472 | 471 | Maggie | 09-18 | 819C8 | 25% glycerol in H2O | 3 | E. coli | Turbos | G209 | O209-8 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 209 | Kan | BI2014, IH6894 | no result (concentration probably was too low), no result (concentration probably was too low) | #N/A | #N/A | |||||
473 | 472 | Silvan | 09-18 | 818C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A176#2 | pSB1C3-H6-Smt3-0ZSortA | 176 | Cm | #N/A | #N/A | #N/A | |||||||
474 | 473 | Silvan | 09-18 | 818C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A176#2 | O176 | pSB1C3-H6-Smt3-0ZSortA | 176 | Cm | AG3764, IH6895 | ok, ok | #N/A | #N/A | |||||
475 | 474 | Silvan | 09-18 | 818C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A176#2 | O176-2 | pSB1C3-H6-Smt3-0ZSortA | 176 | Cm | AG3765, IH6896 | H6R mutation, ok | #N/A | #N/A | |||||
476 | 475 | Silvan | 09-18 | 818C4 | 25% glycerol in H2O | 3 | E. coli | Turbos | A176#2 | pSB1C3-H6-Smt3-0ZSortA | 176 | Cm | #N/A | #N/A | #N/A | |||||||
477 | 476 | Silvan | 09-18 | 818C5 | 25% glycerol in H2O | 3 | E. coli | Turbos | A176#2 | pSB1C3-H6-Smt3-0ZSortA | 176 | Cm | #N/A | #N/A | #N/A | |||||||
478 | 477 | Silvan | 09-18 | 818C7 | 25% glycerol in H2O | 3 | E. coli | Turbos | A176#2 | O176-3 | pSB1C3-H6-Smt3-0ZSortA | 176 | Cm | AG3766, IH6897 | ok, ok | #N/A | #N/A | |||||
479 | 478 | Silvan | 09-18 | 818C8 | 25% glycerol in H2O | 3 | E. coli | Turbos | A176#2 | pSB1C3-H6-Smt3-0ZSortA | 176 | Cm | #N/A | #N/A | #N/A | |||||||
480 | 479 | Lisa | 09-19 | 820C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G210#2 | O210 | pSB1C3-BsaI-CC-O-IasB-Ssra | 210 | Cm | #N/A | #N/A | |||||||
481 | 480 | Lisa | 09-19 | 820C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G210#2 | O210-2 | pSB1C3-BsaI-CC-O-IasB-Ssra | 210 | Cm | Bl1963 | #N/A | #N/A | ||||||
482 | 481 | Lisa | 09-19 | 820C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G210#2 | O210-3 | pSB1C3-BsaI-CC-O-IasB-Ssra | 210 | Cm | #N/A | #N/A | |||||||
483 | 482 | Lisa | 09-19 | 821C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G211#2 | O211 | pSB1C3-BsaI-CC-O-IasB-MTS | 211 | Cm | #N/A | #N/A | |||||||
484 | 483 | Lisa | 09-19 | 821C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G211#2 | O211-2 | pSB1C3-BsaI-CC-O-IasB-MTS | 211 | Cm | AG3770 | #N/A | #N/A | ||||||
485 | 484 | Lisa | 09-19 | 821C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G211#2 | O211-3 | pSB1C3-BsaI-CC-O-IasB-MTS | 211 | Cm | #N/A | #N/A | |||||||
486 | 485 | Lisa | 09-19 | 822C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G212#2 | O212 | pSB1C3-EX-BsaI-A-NN-IasB-myristoilation | 212 | Cm | #N/A | #N/A | |||||||
487 | 486 | Lisa | 09-19 | 822C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G212#2 | O212-2 | pSB1C3-EX-BsaI-A-NN-IasB-myristoilation | 212 | Cm | AG3771 | #N/A | #N/A | ||||||
488 | 487 | Lisa | 09-19 | 822C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G212#2 | O212-3 | pSB1C3-EX-BsaI-A-NN-IasB-myristoilation | 212 | Cm | #N/A | #N/A | |||||||
489 | 488 | Lisa | 09-19 | 823C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G213#2 | O213 | pSB1C3-EX-BsaI-A-NN-IasB-NLS | 213 | Cm | #N/A | #N/A | |||||||
490 | 489 | Lisa | 09-19 | 823C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G213#2 | O213-2 | pSB1C3-EX-BsaI-A-NN-IasB-NLS | 213 | Cm | AG3772 | #N/A | #N/A | ||||||
491 | 490 | Lisa | 09-19 | 823C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G213#2 | O213-3 | pSB1C3-EX-BsaI-A-NN-IasB-NLS | 213 | Cm | #N/A | #N/A | |||||||
492 | 491 | Lisa | 09-19 | 824C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G214#2 | O214 | pSB1C3-EX-BsaI-A-NN-IasB-pelB | 214 | Cm | #N/A | #N/A | |||||||
493 | 492 | Lisa | 09-19 | 824C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G214#2 | O214-2 | pSB1C3-EX-BsaI-A-NN-IasB-pelB | 214 | Cm | AG3773 | #N/A | #N/A | ||||||
494 | 493 | Lisa | 09-19 | 824C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G214#2 | O214-3 | pSB1C3-EX-BsaI-A-NN-IasB-pelB | 214 | Cm | #N/A | #N/A | |||||||
495 | 494 | Lisa | 09-19 | 825C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G215#2 | O215 | pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES | 215 | Cm | #N/A | #N/A | |||||||
496 | 495 | Lisa | 09-19 | 825C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G215#2 | O215-2 | pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES | 215 | Cm | #N/A | #N/A | |||||||
497 | 496 | Lisa | 09-19 | 825C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G215#2 | O215-3 | pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES | 215 | Cm | #N/A | #N/A | |||||||
498 | 497 | Lisa | 09-19 | 826C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | D2012-2-22P | O218 | pSB1A2-BBa_K103006 | 218 | Amp | #N/A | #N/A | |||||||
499 | 498 | Lisa | 09-19 | 826C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | D2012-2-22P | O218-2 | pSB1A2-BBa_K103006 | 218 | Amp | #N/A | #N/A | |||||||
500 | 499 | Lisa | 09-19 | 826C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | D2012-2-22P | O218-3 | pSB1A2-BBa_K103006 | 218 | Amp | #N/A | #N/A | |||||||
501 | 500 | Jakob | 09-20 | 799C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O145-3 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 | 145 | Cm | #N/A | BI2151, BI2163 | ok, | |||||||
502 | 501 | Silvan | 09-20 | 828C1 | 25% glycerol in H2O | 7 | E. coli | Rosetta (DE3) | O179 | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6, pRARE | 179 | 3.10E+01 | Kan, Cm | #N/A | BI1957, AF9231 | , | ||||||
503 | 502 | MaxW | 09-20 | 840C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O181-3 | pSBX1K3-Xylanase | 181 | Kan | #N/A | BI12214 | ok | |||||||
504 | 503 | MaxW | 09-20 | 841C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O216-2 | pSBX1K3-XylaH6BB | 216 | Kan | #N/A | BI1978 | ok | |||||||
505 | 504 | MaxW | 09-20 | 842C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O217 | pSBX1K3-XylaH6extBB | 217 | Kan | #N/A | BI1979, BF8279 | bad seq, bad seq | |||||||
506 | 505 | Caro | 09-21 | 853C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G228 | O228-2 | pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 | 228 | Cm | BF8274 | mutation im prefix? | #N/A | #N/A | |||||
507 | 506 | Caro | 09-21 | 856C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G192#3 | pSBX4C5-1GateNpuDnaE(N)*-H6 | 192 | Cm | #N/A | #N/A | #N/A | |||||||
508 | 507 | Caro | 09-21 | 857C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G191#3 | O191-3 | pSBX4C5-1GateNpuDnaE(C)*-H6 | 191 | Cm | BF8277 | ok short sequencing | #N/A | #N/A | |||||
509 | 508 | Caro | 09-21 | 843C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | P322 | O220 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 220 | Neo/Kan | BF8261, BF8262, BF8281, BF8282 | , , good, but wrong -> no mutation, good, but wrong -> no mutation | |||||||
510 | 509 | Caro | 09-21 | 844C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | P322-2 | O220-2 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 220 | Neo/Kan | BF8263, BF8264, BF8283, BF8284 | , , ok, good!, ok, good! | |||||||
511 | 510 | Caro | 09-21 | 844C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | P322-2 | O220-3 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 220 | Neo/Kan | BF8265, BF8266, BF8285, BF8286 | , , ok, good!, ok, good! | |||||||
512 | 511 | Caro | 09-22 | 872C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O228 | O228-4 | pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 | 228 | Cm | BF8293 | ok, but one mutation in LacOperon | BF8273 | mutation im prefix? but less mutation than O228-2,use O228-4 | |||||
513 | 512 | Caro | 09-22 | 873C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O229 | O229-2 | pSBX4C5-0GateNpuDnaE_C_mRFP169-225 | 229 | Cm | BF8294 | ok, good | BF8275 | OK good | |||||
514 | 513 | MaxW | 09-24 | 880C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G244 | O244 | pSBX4C5-2Zv2Npu:LaLys+bad1 | 244 | Cm | 28HD73 | ok | #N/A | #N/A | |||||
515 | 514 | MaxW | 09-24 | 880C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G244 | O244-2 | pSBX4C5-2Zv2Npu:LaLys+bad1 | 244 | Cm | #N/A | #N/A | |||||||
516 | 515 | MaxW | 09-24 | 881C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G245 | O245 | pSBX4C5-2Zv2Npu:LaLys+may1 | 245 | Cm | 28HD74 | ok | #N/A | #N/A | |||||
517 | 516 | MaxW | 09-24 | 881C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G245 | O245-2 | pSBX4C5-2Zv2Npu:LaLys+may1 | 245 | Cm | #N/A | #N/A | |||||||
518 | 517 | MaxW | 09-24 | 882C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G246 | O246 | pSBX4C5-2Zv2Npu:LaLys+sgt1 | 246 | Cm | 28HD75 | kacke | #N/A | #N/A | |||||
519 | 518 | MaxW | 09-24 | 882C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G246 | O246-2 | pSBX4C5-2Zv2Npu:LaLys+sgt1 | 246 | Cm | #N/A | #N/A | |||||||
520 | 519 | MaxW | 09-24 | 883C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G247 | O247 | pSBX4C5-2Zv2Npu:LaLys+sgt2 | 247 | Cm | 28HD76 | kacke | #N/A | #N/A | |||||
521 | 520 | MaxW | 09-24 | 883C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G247 | O247-2 | pSBX4C5-2Zv2Npu:LaLys+sgt2 | 247 | Cm | #N/A | #N/A | |||||||
522 | 521 | MaxW | 09-24 | 884C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G248 | O248 | pSBX4C5-2Zv2Npu:LaLys+sho1 | 248 | Cm | 28HD77 | ok | #N/A | #N/A | |||||
523 | 522 | MaxW | 09-24 | 884C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G248 | O248-2 | pSBX4C5-2Zv2Npu:LaLys+sho1 | 248 | Cm | #N/A | #N/A | |||||||
524 | 523 | MaxW | 09-24 | 885C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G249 | O249 | pSBX4C5-2Zv2Npu:LaLys+sho2 | 249 | Cm | 28HD78 | ok | #N/A | #N/A | |||||
525 | 524 | MaxW | 09-24 | 885C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G249 | O249-2 | pSBX4C5-2Zv2Npu:LaLys+sho2 | 249 | Cm | #N/A | #N/A | |||||||
526 | 525 | Maggie | 09-24 | 907C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G232 | O232 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) | 232 | Kan | DD1384, DD1391 | ok, ok | #N/A | #N/A | |||||
527 | 526 | Maggie | 09-24 | 907C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G232 | O232-2 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) | 232 | Kan | DD1385, DD1392 | ok, frameshift mutation behind DNMT1 | #N/A | #N/A | |||||
528 | 527 | Maggie | 09-24 | 908C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G231 | O231 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 231 | Kan | DD1386, DD1393 | , | #N/A | #N/A | |||||
529 | 528 | Maggie | 09-24 | 909C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G231 | O231-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 231 | Kan | #N/A | #N/A | |||||||
530 | 529 | Maggie | 09-24 | 909C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G231 | O231-3 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 231 | Kan | #N/A | #N/A | |||||||
531 | 530 | Maggie | 09-24 | 910C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G239 | O239 | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 | 239 | Kan | #N/A | #N/A | |||||||
532 | 531 | Maggie | 09-24 | 910C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G239 | O239-2 | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 | 239 | Kan | 28HE02 | ok | #N/A | #N/A | |||||
533 | 532 | Maggie | 09-24 | 911C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G239 | O239-3 | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 | 239 | Kan | DD1387, DD139428HE01 | no result, no resultok | #N/A | #N/A | |||||
534 | 533 | Maggie | 09-24 | 911C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G239 | O239-4 | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 | 239 | Kan | #N/A | #N/A | |||||||
535 | 534 | Maggie | 09-24 | 912C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G233 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 233 | Kan | #N/A | #N/A | #N/A | |||||||
536 | 535 | Maggie | 09-24 | 913C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G233 | O233-2 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 233 | Kan | #N/A | #N/A | |||||||
537 | 536 | Maggie | 09-24 | 913C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G233 | O233-3 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 233 | Kan | DD1388, DD1395 | no result, no result | #N/A | #N/A | |||||
538 | 537 | Maggie | 09-24 | 913C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | G233 | O233-4 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 233 | Kan | #N/A | #N/A | |||||||
539 | 538 | Maggie | 09-24 | 917C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G262 | O262 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) | 262 | Kan | DD1389, DD139628HE03, 28HE04 | no result, no resultok, ok | #N/A | #N/A | |||||
540 | 539 | Maggie | 09-24 | 917C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G262 | O262-2 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) | 262 | Kan | #N/A | #N/A | |||||||
541 | 540 | Maggie | 09-24 | 917C4 | 25% glycerol in H2O | 3 | E. coli | Turbos | G262 | O262-3 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) | 262 | Kan | #N/A | #N/A | |||||||
542 | 541 | Maggie | 09-24 | 917C5 | 25% glycerol in H2O | 3 | E. coli | Turbos | G262 | O262-4 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) | 262 | Kan | DD1390, DD1397 | no result, no result | #N/A | #N/A | |||||
543 | 542 | Consti | 09-25 | 923C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O217-2 | pSBX1K3-XylaH6extBB | 217 | Kan | #N/A | BF8280 | ok | |||||||
544 | 543 | Silvan | 09-25 | 931C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G251#2 | O251 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H6 | 251 | Kan | 28HD79 | 96bp deletion | #N/A | #N/A | |||||
545 | 544 | Silvan | 09-25 | 931C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G251#2 | O251-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H6 | 251 | Kan | 28HD80 | 96bp deletion | #N/A | #N/A | |||||
546 | 545 | Silvan | 09-25 | 932C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G252#2 | O252 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H6 | 252 | Kan | 28HD81 | mixture | #N/A | #N/A | |||||
547 | 546 | Silvan | 09-25 | 932C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G252#2 | O252-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H6 | 252 | Kan | 28HD82 | probably ok, but short read; possibly G->V mutation in linker | #N/A | #N/A | |||||
548 | 547 | Silvan | 09-25 | 933C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G253#2 | O253 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6 | 253 | Kan | 28HD83 | ok | #N/A | #N/A | |||||
549 | 548 | Silvan | 09-25 | 933C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G253#2 | O253-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6 | 253 | Kan | 28HD84 | 63bp deletion | #N/A | #N/A | |||||
550 | 549 | Silvan | 09-25 | 934C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S236 | O236 | pSBX1K3-T7RBS-H6-Smt3-0ZSortA | 236 | Kan | 28HD85, 28HD97, 28HD98 | wrong primer, ok; start of the read (backbone) looks a bit strange, ok | #N/A | #N/A | |||||
551 | 550 | Silvan | 09-25 | 934C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S236 | O236-2 | pSBX1K3-T7RBS-H6-Smt3-0ZSortA | 236 | Kan | 28HD86 | wrong primer | #N/A | #N/A | |||||
552 | 551 | Silvan | 09-25 | 935C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S238 | O238 | pSBX1K3-T7RBS-H6-0ZSortA | 238 | Kan | 28HD87, 28HD99, 28HE00 | wrong primer, ok, ok | #N/A | #N/A | |||||
553 | 552 | Silvan | 09-25 | 935C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S238 | O238-2 | pSBX1K3-T7RBS-H6-0ZSortA | 238 | Kan | 28HD88 | wrong primer | #N/A | #N/A | |||||
554 | 553 | Silvan | 09-25 | 936C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S125 | O125-11 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | 28HD89, 28HD90 | ok, ok | #N/A | #N/A | |||||
555 | 554 | Silvan | 09-25 | 936C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S125 | O125-12 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 125 | Cm | #N/A | #N/A | |||||||
556 | 555 | Silvan | 09-25 | 956C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G265 | O265 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6 | 265 | Kan | 28HD91 | single base deletion | #N/A | #N/A | |||||
557 | 556 | Silvan | 09-25 | 956C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G265 | O265-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6 | 265 | Kan | 28HD92 | ok | #N/A | #N/A | |||||
558 | 557 | Silvan | 09-25 | 956C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G265 | O265-3 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6 | 265 | Kan | #N/A | #N/A | |||||||
559 | 558 | Silvan | 09-25 | 957C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G250 | O250 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT) | 250 | Kan | 28HD94 | ok | #N/A | #N/A | |||||
560 | 559 | Silvan | 09-25 | 957C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G250 | O250-2 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT) | 250 | Kan | 28HD95 | single silent mutation | #N/A | #N/A | |||||
561 | 560 | Silvan | 09-25 | 958C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G254 | O254 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT) | 254 | Kan | 28HD96 | sequence ok; signal intensity decreased after DNMT1 | #N/A | #N/A | |||||
562 | 561 | Silvan | 09-25 | 958C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G254 | O254-2 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT) | 254 | Kan | 28HD93 | ok | #N/A | #N/A | |||||
563 | 562 | Silvan | 09-25 | 958C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G254 | O254-3 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT) | 254 | Kan | #N/A | #N/A | |||||||
564 | 563 | Jakob | 09-26 | 964C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O180-10 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 180 | Kan | #N/A | DD1410 | OK, mutagenesis worked | |||||||
565 | 564 | Lisa | 09-26 | 946C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G263 | O263 | pSB1C3-EX-BsaI-A-NN-IasB-OmpA | 263 | Cm | 28HE23, 28HE24 | , | #N/A | #N/A | |||||
566 | 565 | Lisa | 09-26 | 946C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G263 | O263-2 | pSB1C3-EX-BsaI-A-NN-IasB-OmpA | 263 | Cm | DD1411, DD141228HE25, 28HE26 | , ok, ok | #N/A | #N/A | |||||
567 | 566 | Caro | 09-26 | 950C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O224 | O224-4 | pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 | 224 | Cm | BF8268 | ok good | |||||||
568 | 567 | Caro | 09-26 | 950C3 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O224 | O224-5 | pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 | 224 | Cm | BF8268 | ok good | |||||||
569 | 568 | Caro | 09-26 | 951C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O225-3 | O225-4 | pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 | 225 | Cm | BF8288 | no mRFPc insert | |||||||
570 | 569 | Caro | 09-26 | 951C3 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O225-3 | O225-5 | pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 | 225 | Cm | BF8288 | no mRFPc insert | |||||||
571 | 570 | Caro | 09-26 | 952C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O226-4 | O226-5 | pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 | 226 | Cm | BF8290 | ok, good | |||||||
572 | 571 | Caro | 09-26 | 952C3 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O226-4 | O226-6 | pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 | 226 | Cm | BF8290 | ok, good | |||||||
573 | 572 | Caro | 09-26 | 953C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O230 | O230-3 | pSBX4C5-0GateNpuDnaE_N_mRFP1-168 | 230 | Cm | BF8276 | mutation in mRFP G toD | |||||||
574 | 573 | Caro | 09-26 | 949C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O223-2 | O223-5 | pSBX4C5-0GateSspDnaX-S11C_mRFP169-225 | 223 | Cm | |||||||||
575 | 574 | MaxW | 09-27 | 965C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G284 | O284 | pSBX1K3-2Zv2Npu:LaLys+bad1 | 284 | Kan | 28HE49 | no insert | #N/A | #N/A | |||||
576 | 575 | MaxW | 09-27 | 965C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G284 | O284-2 | pSBX1K3-2Zv2Npu:LaLys+bad1 | 284 | Kan | #N/A | #N/A | |||||||
577 | 576 | MaxW | 09-27 | 966C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G285 | O285 | pSBX1K3-2Zv2Npu:LaLys+may1 | 285 | Kan | 28HE50 | ok | #N/A | #N/A | |||||
578 | 577 | MaxW | 09-27 | 966C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G285 | O285-2 | pSBX1K3-2Zv2Npu:LaLys+may1 | 285 | Kan | #N/A | #N/A | |||||||
579 | 578 | MaxW | 09-27 | 967C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G286 | O286 | pSBX1K3-2Zv2Npu:LaLys+sgt1 | 286 | Kan | 28HE51 | no insert | #N/A | #N/A | |||||
580 | 579 | MaxW | 09-27 | 967C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G286 | O286-2 | pSBX1K3-2Zv2Npu:LaLys+sgt1 | 286 | Kan | #N/A | #N/A | |||||||
581 | 580 | MaxW | 09-27 | 968C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G287 | O287 | pSBX1K3-2Zv2Npu:LaLys+sgt2 | 287 | Kan | 28HE52 | ok | #N/A | #N/A | |||||
582 | 581 | MaxW | 09-27 | 968C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G287 | O287-2 | pSBX1K3-2Zv2Npu:LaLys+sgt2 | 287 | Kan | #N/A | #N/A | |||||||
583 | 582 | MaxW | 09-27 | 969C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G288 | O288 | pSBX1K3-2Zv2Npu:LaLys+sho1 | 288 | Kan | 28HE53 | ok | #N/A | #N/A | |||||
584 | 583 | MaxW | 09-27 | 969C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G288 | O288-2 | pSBX1K3-2Zv2Npu:LaLys+sho1 | 288 | Kan | #N/A | #N/A | |||||||
585 | 584 | MaxW | 09-27 | 970C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G289 | O289 | pSBX1K3-2Zv2Npu:LaLys+sho2 | 289 | Kan | 28HE54 | ok | #N/A | #N/A | |||||
586 | 585 | MaxW | 09-27 | 970C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G289 | O289-2 | pSBX1K3-2Zv2Npu:LaLys+sho2 | 289 | Kan | #N/A | #N/A | |||||||
587 | 586 | MaxW | 09-27 | 971C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G290 | O290 | pSBX1K3-2Zv2Npu:LaLys+flexlinker | 290 | Kan | 28HE55 | ok | #N/A | #N/A | |||||
588 | 587 | MaxW | 09-27 | 971C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G290 | O290-2 | pSBX1K3-2Zv2Npu:LaLys+flexlinker | 290 | Kan | #N/A | #N/A | |||||||
589 | 588 | Jan | 09-28 | 929C3-2 | 25% glycerol in H2O | #N/A | #N/A | #N/A | p1680 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 180 | Kan | #N/A | #N/A | #N/A | |||||||
590 | 589 | Jan | 09-28 | 838C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O219 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular) | 219 | Neo/Kan | #N/A | |||||||||
591 | 590 | Jan | 09-28 | 961C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | P374 | pSBX4C5-2GateNpu*(C)_v2 | 283 | Cm | #N/A | |||||||||
592 | 591 | Jan | 09-28 | 961C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | P374 | O283 | pSBX4C5-2GateNpu*(C)_v2 | 283 | Cm | |||||||||
593 | 592 | Jan | 09-28 | 961C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | P374 | O283-2 | pSBX4C5-2GateNpu*(C)_v2 | 283 | Cm | |||||||||
594 | 593 | Jan | 09-28 | 961C4 | 25% glycerol in H2O | 3 | E. coli | Turbos | P374 | O283-3 | pSBX4C5-2GateNpu*(C)_v2 | 283 | Cm | |||||||||
595 | 594 | Jan | 09-28 | 961C5 | 25% glycerol in H2O | 3 | E. coli | Turbos | P374 | pSBX4C5-2GateNpu*(C)_v2 | 283 | Cm | #N/A | |||||||||
596 | 595 | Caro/Jakob | 09-29 | 983C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A278 | O278 | pSBX1K3-DnaXN-sfGFPn-H6 | 278 | Kan | DD1413 | ok one additional His | #N/A | #N/A | |||||
597 | 596 | Caro/Jakob | 09-29 | 983C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A278 | O278-2 | pSBX1K3-DnaXN-sfGFPn-H6 | 278 | Kan | DD1414 | ok two additional His | #N/A | #N/A | |||||
598 | 597 | Caro/Jakob | 09-29 | 983C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A278 | O278-3 | pSBX1K3-DnaXN-sfGFPn-H6 | 278 | Kan | #N/A | #N/A | |||||||
599 | 598 | Caro/Jakob | 09-29 | 984C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A277 | O277 | pSBX1K3-DnaXN*-sfGFPn-H6 | 277 | Kan | DD1415 | ok one additional His+ | #N/A | #N/A | |||||
600 | 599 | Caro/Jakob | 09-29 | 984C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A277 | O277-2 | pSBX1K3-DnaXN*-sfGFPn-H6 | 277 | Kan | DD1416 | okay | #N/A | #N/A | |||||
601 | 600 | Caro/Jakob | 09-29 | 984C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A277 | O277-3 | pSBX1K3-DnaXN*-sfGFPn-H6 | 277 | Kan | #N/A | #N/A | |||||||
602 | 601 | Caro/Jakob | 09-29 | 985C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A276 | O276 | pSBX1K3-SspDnaXc-sfGFP^C-H6 | 276 | Kan | DD1417 | ok? two additional His | #N/A | #N/A | |||||
603 | 602 | Caro/Jakob | 09-29 | 985C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A276 | O276-2 | pSBX1K3-SspDnaXc-sfGFP^C-H6 | 276 | Kan | DD1418 | do not use! bad mutations | #N/A | #N/A | |||||
604 | 603 | Caro/Jakob | 09-29 | 985C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A276 | O276-3 | pSBX1K3-SspDnaXc-sfGFP^C-H6 | 276 | Kan | #N/A | #N/A | |||||||
605 | 604 | Caro/Jakob | 09-29 | 987C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A279 | O279 | pSBX1K3-LOV-BsaIIasB-sfGFPc | 279 | Kan | is most probably O281 | DD1419, DD1435 | could be O281?? checking with next sequencing, changed sequencing or construct, additional His, could be O281?? checking with next sequencing | #N/A | #N/A | ||||
606 | 605 | Caro/Jakob | 09-29 | 987C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A279 | O279-2 | pSBX1K3-LOV-BsaIIasB-sfGFPc | 279 | Kan | is most probably O281-2 | DD1420, DD1436 | could be O281?? checking with next sequencing, changed sequencing or construct, additional His, could be O281?? checking with next sequencing, changed sequencing or construct, additional His | #N/A | #N/A | ||||
607 | 606 | Caro/Jakob | 09-29 | 987C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A279 | O279-3 | pSBX1K3-LOV-BsaIIasB-sfGFPc | 279 | Kan | #N/A | #N/A | |||||||
608 | 607 | Caro/Jakob | 09-29 | 988C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A280 | O280 | pSBX1K3-LOV-12-DnaXC-sfGFPc | 280 | Kan | DD1421, DD1437 | no LOV domain, no LOV domain | #N/A | #N/A | |||||
609 | 608 | Caro/Jakob | 09-29 | 988C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A280 | O280-2 | pSBX1K3-LOV-12-DnaXC-sfGFPc | 280 | Kan | DD1422, DD1438 | one additional His, frame shift in the end, one additional His, frame shift in the end | #N/A | #N/A | |||||
610 | 609 | Caro/Jakob | 09-29 | 988C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A280 | O280-3 | pSBX1K3-LOV-12-DnaXC-sfGFPc | 280 | Kan | 28HE14, 28HE18 | ok, good, ok, good | #N/A | #N/A | |||||
611 | 610 | Caro/Jakob | 09-29 | 989C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A281 | O281 | pSBX1K3-LOV-6-DnaXC-sfGFPc | 281 | Kan | is most probably O279 | DD1423, DD1439 | could be O279?? checking with next sequencing, changed sequencing or construct, additional His, could be O279?? checking with next sequencing, changed sequencing or construct, additional His | #N/A | #N/A | ||||
612 | 611 | Caro/Jakob | 09-29 | 989C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A281 | O281-2 | pSBX1K3-LOV-6-DnaXC-sfGFPc | 281 | Kan | most probably O279-2 | DD1424, DD1440 | could be O279?? checking with next sequencing, changed sequencing or construct, additional His, could be O279?? checking with next sequencing, changed sequencing or construct, additional His | #N/A | #N/A | ||||
613 | 612 | Caro/Jakob | 09-29 | 989C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A281 | O281-3 | pSBX1K3-LOV-6-DnaXC-sfGFPc | 281 | Kan | #N/A | #N/A | |||||||
614 | 613 | Caro/Jakob | 09-29 | 990C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A267 | O267 | pSBX1K3-H6-mRFPn-DnaXN-H6 | 267 | Kan | DD1425 | bad read at the beginning, probably frame shift mutations in between | #N/A | #N/A | |||||
615 | 614 | Caro/Jakob | 09-29 | 990C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A267 | O267-2 | pSBX1K3-H6-mRFPn-DnaXN-H6 | 267 | Kan | DD1426 | turned out to be pSBX1K3-sfGFPn-NpuDnaEN | #N/A | #N/A | |||||
616 | 615 | Caro/Jakob | 09-29 | 990C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A267 | O267-3 | pSBX1K3-H6-mRFPn-DnaXN-H6 | 267 | Kan | 28HE15 | ok, good | #N/A | #N/A | |||||
617 | 616 | Caro/Jakob | 09-29 | 991C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A268 | O268 | pSBX1K3-H6-mRFPn-DnaXN*-H6 | 268 | Kan | DD1427 | Intein DnaXN insert not included | #N/A | #N/A | |||||
618 | 617 | Caro/Jakob | 09-29 | 991C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A268 | O268-2 | pSBX1K3-H6-mRFPn-DnaXN*-H6 | 268 | Kan | DD1428 | Intein DnaXN insert not included | #N/A | #N/A | |||||
619 | 618 | Caro/Jakob | 09-29 | 991C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A268 | O268-3 | pSBX1K3-H6-mRFPn-DnaXN*-H6 | 268 | Kan | 28HE16 | ok, good | #N/A | #N/A | |||||
620 | 619 | Caro/Jakob | 09-29 | 992C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A269 | O269 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 | 269 | Kan | DD1429, DD1441 | frame shift between His, | #N/A | #N/A | |||||
621 | 620 | Caro/Jakob | 09-29 | 992C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A269 | O269-2 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 | 269 | Kan | DD1430, DD1442 | ok one additional His, | #N/A | #N/A | |||||
622 | 621 | Caro/Jakob | 09-29 | 992C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A269 | O269-3 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 | 269 | Kan | #N/A | #N/A | |||||||
623 | 622 | Caro/Jakob | 09-29 | 993C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A270 | O270 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 | 270 | Kan | DD1431, DD1443 | okay, | #N/A | #N/A | |||||
624 | 623 | Caro/Jakob | 09-29 | 993C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A270 | O270-2 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 | 270 | Kan | DD1432, DD1444 | okay, one point mutation in Lac Operator, | #N/A | #N/A | |||||
625 | 624 | Caro/Jakob | 09-29 | 993C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A270 | O270-3 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 | 270 | Kan | #N/A | #N/A | |||||||
626 | 625 | Caro/Jakob | 09-29 | 994C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A271 | O271 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 271 | Kan | DD1433, DD1445 | 6 amino acids missing in helixintein construct, | #N/A | #N/A | |||||
627 | 626 | Caro/Jakob | 09-29 | 994C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A271 | O271-2 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 271 | Kan | DD1434, DD1446 | LOV domain missing, | #N/A | #N/A | |||||
628 | 627 | Caro/Jakob | 09-29 | 996C1 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A273 | O273 | pSBX1K3-H6-DnaXC*-mRFPc-H6 | 273 | Kan | 28HE71 | is pSBX1K3-H6-DnaXC-mRFPc-H6, mutation H->Q ?, short read | #N/A | #N/A | |||||
629 | 628 | Caro/Jakob | 09-29 | 996C2 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A273 | O273-2 | pSBX1K3-H6-DnaXC*-mRFPc-H6 | 273 | Kan | 28HE72 | bad read, end of mRFP okay | #N/A | #N/A | |||||
630 | 629 | Caro/Jakob | 09-29 | 996C3 | 25% glycerol in H2O | 1 | E. coli | TOP10 | A273 | O273-3 | pSBX1K3-H6-DnaXC*-mRFPc-H6 | 273 | Kan | 28HE17 | many mutations | #N/A | #N/A | |||||
631 | 630 | Jan | 1072C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G327 | O327 | pSBX4C5-1GateGp41-1(N)-H6 | 327 | Cm | DD1447 | ok | #N/A | #N/A | ||||||
632 | 631 | Jan | 1073C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G328 | O328 | pSBX4C5-H6-1GateGp41-1(C) | 328 | Cm | DD1449 | ok | #N/A | #N/A | ||||||
633 | 632 | Jan | 1074C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G329 | O329 | pSBX4C5-DoubleGateGp41-1(N) | 329 | Cm | DD1451, DD1459 | ok, not completely sequenced, ok, not completely sequenced | #N/A | #N/A | ||||||
634 | 633 | Jan | 1075C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G330 | O330 | pSBX4C5-DoubleGateGp41-1(C) | 330 | Cm | DD1453, DD1461 | ok, not completely sequenced, ok, not completely sequenced | #N/A | #N/A | ||||||
635 | 634 | Jan | 1076C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G331 | O331 | pSBX4C5-DoubleNpuDnaE(N) | 331 | Cm | DD1455, DD1463 | sequencing not long enough, poor sequenzing could be ok intein not sequenced | #N/A | #N/A | ||||||
636 | 635 | Jan | 1077C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G332 | O332 | pSBX4C5-DoubleGateNpuDnaE(C) | 332 | Cm | DD1457, DD1465 | sequencing not long enough, intein not sequenced, short sequenzin but ok | #N/A | #N/A | ||||||
637 | 636 | MaxW | 09-29 | 1006C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G291 | O291 | pSBX1K3-2Zv2Npu:LaLys+bad2 | 291 | Kan | 28HE56 | bad | #N/A | #N/A | |||||
638 | 637 | MaxW | 09-29 | 1006C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G291 | O291-2 | pSBX1K3-2Zv2Npu:LaLys+bad2 | 291 | Kan | #N/A | #N/A | |||||||
639 | 638 | MaxW | 09-29 | 1007C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G292 | O292 | pSBX1K3-2Zv2Npu:LaLys+bad3 | 292 | Kan | 28HE57 | bad | #N/A | #N/A | |||||
640 | 639 | MaxW | 09-29 | 1007C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G292 | O292-2 | pSBX1K3-2Zv2Npu:LaLys+bad3 | 292 | Kan | #N/A | #N/A | |||||||
641 | 640 | MaxW | 09-29 | 1008C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G293 | O293 | pSBX1K3-2Zv2Npu:LaLys+bad4 | 293 | Kan | 28HE58 | bad | #N/A | #N/A | |||||
642 | 641 | MaxW | 09-29 | 1008C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G293 | O293-2 | pSBX1K3-2Zv2Npu:LaLys+bad4 | 293 | Kan | #N/A | #N/A | |||||||
643 | 642 | MaxW | 09-29 | 1009C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G294 | O294 | pSBX1K3-2Zv2Npu:LaLys+may2 | 294 | Kan | 28HE59 | no insert | #N/A | #N/A | |||||
644 | 643 | MaxW | 09-29 | 1009C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G294 | O294-2 | pSBX1K3-2Zv2Npu:LaLys+may2 | 294 | Kan | #N/A | #N/A | |||||||
645 | 644 | MaxW | 09-29 | 1010C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G295 | O295 | pSBX1K3-2Zv2Npu:LaLys+may3 | 295 | Kan | 28HE60 | half insert | #N/A | #N/A | |||||
646 | 645 | MaxW | 09-29 | 1010C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G295 | O295-2 | pSBX1K3-2Zv2Npu:LaLys+may3 | 295 | Kan | #N/A | #N/A | |||||||
647 | 646 | MaxW | 09-29 | 1011C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G296 | O296 | pSBX1K3-2Zv2Npu:LaLys+ord1 | 296 | Kan | #N/A | #N/A | |||||||
648 | 647 | MaxW | 09-29 | 1011C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G296 | O296-2 | pSBX1K3-2Zv2Npu:LaLys+ord1 | 296 | Kan | 28HE61 | ok | #N/A | #N/A | |||||
649 | 648 | MaxW | 09-29 | 1012C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G297 | O297 | pSBX1K3-2Zv2Npu:LaLys+ord2 | 297 | Kan | 28HE62 | deletions | #N/A | #N/A | |||||
650 | 649 | MaxW | 09-29 | 1012C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G297 | O297-2 | pSBX1K3-2Zv2Npu:LaLys+ord2 | 297 | Kan | #N/A | #N/A | |||||||
651 | 650 | MaxW | 09-29 | 1013C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G298 | O298 | pSBX1K3-2Zv2Npu:LaLys+ord3 | 298 | Kan | 28HE63 | ok | #N/A | #N/A | |||||
652 | 651 | MaxW | 09-29 | 1013C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G298 | O298-2 | pSBX1K3-2Zv2Npu:LaLys+ord3 | 298 | Kan | #N/A | #N/A | |||||||
653 | 652 | MaxW | 09-29 | 1014C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G299 | O299 | pSBX1K3-2Zv2Npu:LaLys+ord4 | 299 | Kan | 28HE64 | half insert | #N/A | #N/A | |||||
654 | 653 | MaxW | 09-29 | 1014C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G299 | O299-2 | pSBX1K3-2Zv2Npu:LaLys+ord4 | 299 | Kan | #N/A | #N/A | |||||||
655 | 654 | MaxW | 09-29 | 1015C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G300 | O300 | pSBX1K3-2Zv2Npu:LaLys+ord5 | 300 | Kan | 28HE65 | no insert | #N/A | #N/A | |||||
656 | 655 | MaxW | 09-29 | 1015C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G300 | O300-2 | pSBX1K3-2Zv2Npu:LaLys+ord5 | 300 | Kan | #N/A | #N/A | |||||||
657 | 656 | MaxW | 09-29 | 1016C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G301 | O301 | pSBX1K3-2Zv2Npu:LaLys+ord6 | 301 | Kan | 28HE66 | no insert | #N/A | #N/A | |||||
658 | 657 | MaxW | 09-29 | 1016C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G301 | O301-2 | pSBX1K3-2Zv2Npu:LaLys+ord6 | 301 | Kan | #N/A | #N/A | |||||||
659 | 658 | MaxW | 09-29 | 1017C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G302 | O302 | pSBX1K3-2Zv2Npu:LaLys+sgt3 | 302 | Kan | 28HE67 | no insert | #N/A | #N/A | |||||
660 | 659 | MaxW | 09-29 | 1017C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G302 | O302-2 | pSBX1K3-2Zv2Npu:LaLys+sgt3 | 302 | Kan | #N/A | #N/A | |||||||
661 | 660 | MaxW | 09-29 | 1018C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G303 | O303 | pSBX1K3-2Zv2Npu:LaLys+sgt4 | 303 | Kan | 28HE68 | no insert | #N/A | #N/A | |||||
662 | 661 | MaxW | 09-29 | 1018C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G303 | O303-2 | pSBX1K3-2Zv2Npu:LaLys+sgt4 | 303 | Kan | #N/A | #N/A | |||||||
663 | 662 | MaxW | 09-29 | 1019C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G304 | O304 | pSBX1K3-2Zv2Npu:LaLys+sho3 | 304 | Kan | 28HE69 | no insert | #N/A | #N/A | |||||
664 | 663 | MaxW | 09-29 | 1019C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G304 | O304-2 | pSBX1K3-2Zv2Npu:LaLys+sho3 | 304 | Kan | #N/A | #N/A | |||||||
665 | 664 | MaxW | 09-29 | 1020C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G305 | O305 | pSBX1K3-2Zv2Npu:LaLys+rigid_RGT | 305 | Kan | 28HE70 | insertions | #N/A | #N/A | |||||
666 | 665 | MaxW | 09-29 | 1020C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G305 | O305-2 | pSBX1K3-2Zv2Npu:LaLys+rigid_RGT | 305 | Kan | #N/A | #N/A | |||||||
667 | 666 | Schorly | 09-29 | 1044C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O285 | pSBX1K3-2Zv2Npu:LaLys+may1 | 285 | Kan | #N/A | 28HE50 | ok | |||||||
668 | 667 | Schorly | 09-29 | 1046C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O287 | pSBX1K3-2Zv2Npu:LaLys+sgt2 | 287 | Kan | #N/A | 28HE52 | ok | |||||||
669 | 668 | Schorly | 09-29 | 1047C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O288 | pSBX1K3-2Zv2Npu:LaLys+sho1 | 288 | Kan | #N/A | 28HE53 | ok | |||||||
670 | 669 | Schorly | 09-29 | 1048C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O289 | pSBX1K3-2Zv2Npu:LaLys+sho2 | 289 | Kan | #N/A | 28HE54 | ok | |||||||
671 | 670 | Schorly | 09-29 | 1049C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O290 | pSBX1K3-2Zv2Npu:LaLys+flexlinker | 290 | Kan | #N/A | 28HE55 | ok | |||||||
672 | 671 | Konrad Herbst | 10-01 | KH38.2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O364-2 | 364 | from Konrad Herbst | |||||||||||
673 | 672 | Konrad Herbst | 10-01 | KH39PstI.1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O365-2 | 365 | from Konrad Herbst | |||||||||||
674 | 673 | Konrad Herbst | 10-01 | KH39.2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O366-2 | 366 | from Konrad Herbst | |||||||||||
675 | 674 | Jan | 1072C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G327 | O327-2 | pSBX4C5-1GateGp41-1(N)-H6 | 327 | Cm | DD1448 | will be repeated | #N/A | #N/A | ||||||
676 | 675 | Jan | 1073C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G328 | O328-2 | pSBX4C5-H6-1GateGp41-1(C) | 328 | Cm | DD1450 | ok | #N/A | #N/A | ||||||
677 | 676 | Jan | 1074C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G329 | O329-2 | pSBX4C5-DoubleGateGp41-1(N) | 329 | Cm | DD1452, DD1460 | bad sequenzing, bad sequenzing | #N/A | #N/A | ||||||
678 | 677 | Jan | 1075C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G330 | O330-2 | pSBX4C5-DoubleGateGp41-1(C) | 330 | Cm | DD1454, DD1462 | insertion in the prefix, mutation in mRFP, poor sequenzing, but ok | #N/A | #N/A | ||||||
679 | 678 | Jan | 1076C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G331 | O331-2 | pSBX4C5-DoubleNpuDnaE(N) | 331 | Cm | DD1456, DD1464 | sequencing not long enough, ok intein not sequenzed | #N/A | #N/A | ||||||
680 | 679 | Jan | 1077C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | G332 | O332-2 | pSBX4C5-DoubleGateNpuDnaE(C) | 332 | Cm | DD1458, DD1466 | poor sequenzing, especially in the beginningg, but no indication that it was not ok, poor sequrnzing could be ok | #N/A | #N/A | ||||||
681 | 680 | Caro | 1066C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G310 | O310 | pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-225 | 310 | Cm | most probably 308 | 28HE08, 28HE21 | ok good, but not 310, DnaXC* inside (O308), ok, good but is actually DnaXC*-mRFPc (O308) | #N/A | #N/A | |||||
682 | 681 | Caro | 1067C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G311 | O311 | pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-168 | 311 | Cm | most probably 309 | 28HE09, 28HE35 | ok good, but not 312 inside, DnaXN* inside (O309-2), ok, good, but actually DnaXN*-mRFPn (O309) | #N/A | #N/A | |||||
683 | 682 | Caro | 1068C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G312 | O312 | pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 | 312 | Cm | most probably 310 | 28HE10, 28HE36 | ok good, but GyrBC* (O310) inside , ok , good, but actually GyrBC*-mRFPc (O310) | #N/A | #N/A | |||||
684 | 683 | Caro | 1069C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G313 | O313 | pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 | 313 | Cm | most probably 311 | 28HE11, 28HE37 | ok good, but GyrBN* (O311) inside, ok, good, but actually GyrBN*-mRFPn (O311) | #N/A | #N/A | |||||
685 | 684 | Caro | 1037C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A272 | O272 | pSBX1K3-H6-DnaXC-mRFPc-H6 | 272 | Kan | most probably 273 | 28HE44, 28HE19 | actually pSBX1K3-H6-DnaXC*-mRFPc-H6, construct or sequencing changed?, ok good, but actually O273 | #N/A | #N/A | |||||
686 | 685 | Jan | 1072C3 | 25% glycerol in H2O | #N/A | #N/A | #N/A | G327 | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | |||||||
687 | 686 | Jan | 1073C3 | 25% glycerol in H2O | #N/A | #N/A | #N/A | G328 | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | |||||||
688 | 687 | Jan | 1074C3 | 25% glycerol in H2O | #N/A | #N/A | #N/A | G329 | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | |||||||
689 | 688 | Jan | 1075C3 | 25% glycerol in H2O | #N/A | #N/A | #N/A | G330 | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | |||||||
690 | 689 | Jan | 1076C3 | 25% glycerol in H2O | #N/A | #N/A | #N/A | G331 | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | |||||||
691 | 690 | Jan | 1077C3 | 25% glycerol in H2O | #N/A | #N/A | #N/A | G332 | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | #N/A | |||||||
692 | 691 | Caro | 1063C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G227#2 | O227-5 | pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 | 227 | Cm | 28HE05 | good, ok | #N/A | #N/A | ||||||
693 | 692 | Lisa | 10-03 | 1078C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A240#5 | O240 | pSBX4C5-2GateSspDnaB(C) | 240 | Cm | DD1467 | ok | #N/A | #N/A | |||||
694 | 693 | Lisa | 10-03 | 1078C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A240#5 | O240-2 | pSBX4C5-2GateSspDnaB(C) | 240 | Cm | #N/A | #N/A | |||||||
695 | 694 | Lisa | 10-03 | 1078C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A240#5 | O240-3 | pSBX4C5-2GateSspDnaB(C) | 240 | Cm | DD1468 | ok (maybe there is something in the beginning of mRFP) | #N/A | #N/A | |||||
696 | 695 | Lisa | 10-03 | 1079C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A241#5 | O241 | pSBX4C5-2GateSspDnaB(C)* | 241 | Cm | #N/A | #N/A | |||||||
697 | 696 | Lisa | 10-03 | 1079C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A241#5 | O241-2 | pSBX4C5-2GateSspDnaB(C)* | 241 | Cm | DD1469 | ok | #N/A | #N/A | |||||
698 | 697 | Lisa | 10-03 | 1079C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A241#5 | O241-3 | pSBX4C5-2GateSspDnaB(C)* | 241 | Cm | DD1470 | ok | #N/A | #N/A | |||||
699 | 698 | Lisa | 10-03 | 1080C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A242#5 | O242 | pSBX4C5-2GateSspDnaB(N) | 242 | Cm | DD1471 | perfect mRFP read, call GATC | #N/A | #N/A | |||||
700 | 699 | Lisa | 10-03 | 1080C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A242#5 | O242-2 | pSBX4C5-2GateSspDnaB(N) | 242 | Cm | DD1472 | perfect mRFP read, call GATC | #N/A | #N/A | |||||
701 | 700 | Lisa | 10-03 | 1080C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A242#5 | O242-3 | pSBX4C5-2GateSspDnaB(N) | 242 | Cm | #N/A | #N/A | |||||||
702 | 701 | Lisa | 10-03 | 1081C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A243#5 | O243 | pSBX4C5-2GateSspDnaB(N)* | 243 | Cm | #N/A | #N/A | |||||||
703 | 702 | Lisa | 10-03 | 1081C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A243#5 | O243-2 | pSBX4C5-2GateSspDnaB(N)* | 243 | Cm | DD1473 | perfect mRFP read, call GATC | #N/A | #N/A | |||||
704 | 703 | Lisa | 10-03 | 1081C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A243#5 | O243-3 | pSBX4C5-2GateSspDnaB(N)* | 243 | Cm | DD1474 | perfect mRFP read, call GATC | #N/A | #N/A | |||||
705 | 704 | Lisa | 10-03 | 1082C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A325#2 | O325 | pSBX4C5-2GateSspDnaE(N) | 325 | Cm | DD1475, | perfect mRFP read, call GATC, | #N/A | #N/A | |||||
706 | 705 | Lisa | 10-03 | 1082C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A325#2 | O325-2 | pSBX4C5-2GateSspDnaE(N) | 325 | Cm | DD1476, | perfect mRFP read, call GATC, | #N/A | #N/A | |||||
707 | 706 | Lisa | 10-03 | 1082C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A325#2 | O325-3 | pSBX4C5-2GateSspDnaE(N) | 325 | Cm | #N/A | #N/A | |||||||
708 | 707 | Lisa | 10-03 | 1083C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A326#2 | O326 | pSBX4C5-2GateSspDnaE(C) | 326 | Cm | DD1477 | double insert insertion | #N/A | #N/A | |||||
709 | 708 | Lisa | 10-03 | 1083C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A326#2 | O326-2 | pSBX4C5-2GateSspDnaE(C) | 326 | Cm | #N/A | #N/A | |||||||
710 | 709 | Lisa | 10-03 | 1083C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A326#2 | O326-3 | pSBX4C5-2GateSspDnaE(C) | 326 | Cm | DD1478 | tripple insert insertion | #N/A | #N/A | |||||
711 | 710 | Lisa | 10-03 | 1090C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G346 | pSB1C3-EX-BsaI-CBD | 346 | Cm | #N/A | #N/A | #N/A | |||||||
712 | 711 | Lisa | 10-03 | 1090C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G346 | O346 | pSB1C3-EX-BsaI-CBD | 346 | Cm | DD1479 | no.....GG could not work | #N/A | #N/A | |||||
713 | 712 | Lisa | 10-03 | 1090C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G346 | pSB1C3-EX-BsaI-CBD | 346 | Cm | #N/A | #N/A | #N/A | |||||||
714 | 713 | Lisa | 10-03 | 1090C4 | 25% glycerol in H2O | 3 | E. coli | Turbos | G346 | O346-2 | pSB1C3-EX-BsaI-CBD | 346 | Cm | DD1480 | no.....GG could not work | #N/A | #N/A | |||||
715 | 714 | Lisa | 10-03 | 1090C5 | 25% glycerol in H2O | 3 | E. coli | Turbos | G346 | O346-3 | pSB1C3-EX-BsaI-CBD | 346 | Cm | 28HE43 | no.....GG could not work | #N/A | #N/A | |||||
716 | 715 | Charlotte | today | 1114C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O296-2 | pSBX1K3-2Zv2Npu:LaLys+ord1 | 296 | Kan | #N/A | 28HE61 | ok | |||||||
717 | 716 | Charlotte | today | 1115C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O298 | pSBX1K3-2Zv2Npu:LaLys+ord3 | 298 | Kan | #N/A | 28HE63 | ok | |||||||
718 | 717 | MaxW | 10-03 | 1097C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S347 | O347 | pSB1C3-2Zv2Npu | 347 | Cm | #N/A | #N/A | |||||||
719 | 718 | MaxW | 10-03 | 1098C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S348 | O348 | pSB1C3-T7RBS-LambdaLys-IGEMHD_v3 | 348 | Cm | #N/A | #N/A | |||||||
720 | 719 | MaxW | 10-03 | 1099C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S349 | O349 | pSB1C3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 | 349 | Cm | #N/A | #N/A | |||||||
721 | 720 | MaxW | 10-03 | 1100C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S350 | O350 | pSB1C3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 | 350 | Cm | #N/A | #N/A | |||||||
722 | 721 | MaxW | 10-03 | 1101C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S351 | O351 | pSB1C3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 | 351 | Cm | #N/A | #N/A | |||||||
723 | 722 | MaxW | 10-03 | 1102C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S352 | O352 | pSB1C3-2Zv2Npu:(LambdaLys-H6) | 352 | Cm | #N/A | #N/A | |||||||
724 | 723 | MaxW | 10-03 | 1103C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S353 | O353 | pSB1C3-2Zv2Npu:(LambdaLys-Rigid) | 353 | Cm | #N/A | #N/A | |||||||
725 | 724 | MaxW | 10-03 | 1104C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S354 | O354 | pSB1C3-2Zv2Npu:Xyla_BsaI_circ_His | 354 | Cm | #N/A | #N/A | |||||||
726 | 725 | MaxW | 10-03 | 1105C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S355 | O355 | pSB1C3-2Zv2Npu:Xyla_circ_noHis | 355 | Cm | #N/A | #N/A | |||||||
727 | 726 | MaxW | 10-03 | 1106C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S356 | O356 | pSB1C3-1GateNpuDnaE(N) | 356 | Cm | #N/A | #N/A | |||||||
728 | 727 | MaxW | 10-03 | 1107C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S357 | O357 | pSB1C3-1GateNpuDnaE(C) | 357 | Cm | #N/A | #N/A | |||||||
729 | 728 | MaxW | 10-03 | 1108C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S358 | O358 | pSB1C3-2Zv2Npu:Xyla(H6)destrCext | 358 | Cm | #N/A | #N/A | |||||||
730 | 729 | MaxW | 10-03 | 1109C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S359 | O359 | pSB1C3-T7RBS-FLAG-Smt3-2Zv2Npu | 359 | Cm | #N/A | #N/A | |||||||
731 | 730 | MaxW | 10-03 | 1110C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S360 | O360 | pSB1C3-Xylanase | 360 | Cm | #N/A | #N/A | |||||||
732 | 731 | MaxW | 10-03 | 1111C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S361 | O361 | pSB1C3-LiLaLy(H6) | 361 | Cm | #N/A | #N/A | |||||||
733 | 732 | MaxW | 10-03 | 1112C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S362 | O362 | pSB1C3-XylaH6BB | 362 | Cm | #N/A | #N/A | |||||||
734 | 733 | MaxW | 10-03 | 1113C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S363 | O363 | pSB1C3-XylaH6extBB | 363 | Cm | #N/A | #N/A | |||||||
735 | 734 | Lisa | 10-04 | 1135C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O240 | O371 | pSBX4C5-1GateSspDnaB(C) | 371 | Cm | DD1467 | ok | |||||||
736 | 735 | Lisa | 10-04 | 1135C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | O240 | O371-2 | pSBX4C5-1GateSspDnaB(C) | 371 | Cm | DD1467 | ok | |||||||
737 | 736 | Lisa | 10-04 | 1136C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O240-3 | O371-3 | pSBX4C5-1GateSspDnaB(C) | 371 | Cm | DD1468 | ok (maybe there is something in the beginning of mRFP) | |||||||
738 | 737 | Lisa | 10-04 | 1136C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O240-3 | O371-4 | pSBX4C5-1GateSspDnaB(C) | 371 | Cm | DD1468 | ok (maybe there is something in the beginning of mRFP) | |||||||
739 | 738 | Lisa | 10-04 | 1137C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O241-2 | O372 | pSBX4C5-1GateSspDnaB(C*) | 372 | Cm | DD1469 | ok | |||||||
740 | 739 | Lisa | 10-04 | 1137C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O241-2 | O372-2 | pSBX4C5-1GateSspDnaB(C*) | 372 | Cm | DD1469 | ok | |||||||
741 | 740 | Lisa | 10-04 | 1138C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O241-3 | O372-3 | pSBX4C5-1GateSspDnaB(C*) | 372 | Cm | DD1470 | ok | |||||||
742 | 741 | Lisa | 10-04 | 1138C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O241-3 | O372-3 | pSBX4C5-1GateSspDnaB(C*) | 372 | Cm | DD1470 | ok | |||||||
743 | 742 | Lisa | 10-04 | 1139C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O242 | O373 | pSBX4C5-1GateSspDnaB(N) | 373 | Cm | DD1471 | perfect mRFP read, call GATC | |||||||
744 | 743 | Lisa | 10-04 | 1139C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | O242 | O373-2 | pSBX4C5-1GateSspDnaB(N) | 373 | Cm | DD1471 | perfect mRFP read, call GATC | |||||||
745 | 744 | Lisa | 10-04 | 1140C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O242-2 | O373-3 | pSBX4C5-1GateSspDnaB(N) | 373 | Cm | DD1472 | perfect mRFP read, call GATC | |||||||
746 | 745 | Lisa | 10-04 | 1140C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O242-2 | O373-4 | pSBX4C5-1GateSspDnaB(N) | 373 | Cm | DD1472 | perfect mRFP read, call GATC | |||||||
747 | 746 | Lisa | 10-04 | 1141C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O243-2 | O374 | pSBX4C5-1GateSspDnaB(N*) | 374 | Cm | DD1473 | perfect mRFP read, call GATC | |||||||
748 | 747 | Lisa | 10-04 | 1141C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | O243-2 | O374-2 | pSBX4C5-1GateSspDnaB(N*) | 374 | Cm | DD1473 | perfect mRFP read, call GATC | |||||||
749 | 748 | Lisa | 10-04 | 1143C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O325 | O369 | pSBX4C5-1GateSspDnaE(N) | 369 | Cm | DD1475, | perfect mRFP read, call GATC, | |||||||
750 | 749 | Lisa | 10-04 | 1143C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O325 | O369-2 | pSBX4C5-1GateSspDnaE(N) | 369 | Cm | DD1475, | perfect mRFP read, call GATC, | |||||||
751 | 750 | Lisa | 10-04 | 1144C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O325-2 | O369-3 | pSBX4C5-1GateSspDnaE(N) | 369 | Cm | DD1476, | perfect mRFP read, call GATC, | |||||||
752 | 751 | Lisa | 10-04 | 1144C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | O325-2 | O369-4 | pSBX4C5-1GateSspDnaE(N) | 369 | Cm | DD1476, | perfect mRFP read, call GATC, | |||||||
753 | 752 | Lisa | 10-04 | 1145C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O326 | O370 | pSBX4C5-1GateSspDnaE(C) | 370 | Cm | DD1477 | double insert insertion | |||||||
754 | 753 | Lisa | 10-04 | 1145C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | O326 | O370-2 | pSBX4C5-1GateSspDnaE(C) | 370 | Cm | DD1477 | double insert insertion | |||||||
755 | 754 | Lisa | 10-04 | 1146C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | O326-3 | O370-3 | pSBX4C5-1GateSspDnaE(C) | 370 | Cm | DD1478 | tripple insert insertion | |||||||
756 | 755 | Lisa | 10-04 | 1146C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | O326-3 | O370-4 | pSBX4C5-1GateSspDnaE(C) | 370 | Cm | DD1478 | tripple insert insertion | |||||||
757 | 756 | MaxW | 10-04 | 1116C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S375 | O375 | pSB1C3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 | 375 | Cm | #N/A | #N/A | |||||||
758 | 757 | MaxW | 10-04 | 1117C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S376 | O376 | pSB1C3-1Gate-NpuDnaE(N)-H6 | 376 | Cm | #N/A | #N/A | |||||||
759 | 758 | MaxW | 10-04 | 1118C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S377 | O377 | pSB1C3-H6-1GateNpuDnaE(C) | 377 | Cm | #N/A | #N/A | |||||||
760 | 759 | MaxW | 10-04 | 1119C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S378 | O378 | pSB1C3-T7RBS-H6-sfGFP_T65C-H6 | 378 | Cm | #N/A | #N/A | |||||||
761 | 760 | MaxW | 10-04 | 1120C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S379 | O379 | pSB1C3-1GateSspDnaX-S11(C)-H6 | 379 | Cm | #N/A | #N/A | |||||||
762 | 761 | MaxW | 10-04 | 1121C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S380 | O380 | pSB1C3-1GateSspDnaX-S11N_H6 | 380 | Cm | #N/A | #N/A | |||||||
763 | 762 | MaxW | 10-04 | 1122C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S381 | O381 | pSB1C3-1GateSspGyrB-S11N_H6 | 381 | Cm | #N/A | #N/A | |||||||
764 | 763 | MaxW | 10-04 | 1123C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S382 | O382 | pSB1C3-1GateTerDnaE3-S11C_H6 | 382 | Cm | #N/A | #N/A | |||||||
765 | 764 | MaxW | 10-04 | 1124C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S383 | O383 | pSB1C3-1GateTerDnaE3-S11(N)-H6 | 383 | Cm | #N/A | #N/A | |||||||
766 | 765 | Caro | 10-05 | 1036C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A271 | O271-3 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 271 | Kan | DG7164 | #N/A | #N/A | ||||||
767 | 766 | Caro | 10-05 | 1036C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A271 | O271-4 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 271 | Kan | DG7165 | #N/A | #N/A | ||||||
768 | 767 | Caro | 10-05 | 1036C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A271 | O271-5 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 271 | Kan | #N/A | #N/A | |||||||
769 | 768 | Caro | 10-05 | 1095C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A272#3 | O272-4 | pSBX1K3-H6-DnaXC-mRFPc-H6 | 272 | Kan | DG7162 | #N/A | #N/A | ||||||
770 | 769 | Caro | 10-05 | 1095C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A272#3 | O272-5 | pSBX1K3-H6-DnaXC-mRFPc-H6 | 272 | Kan | DG7163 | #N/A | #N/A | ||||||
771 | 770 | Caro | 10-05 | 1095C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A272#3 | O272-6 | pSBX1K3-H6-DnaXC-mRFPc-H6 | 272 | Kan | #N/A | #N/A | |||||||
772 | 771 | Caro | 10-05 | 1096C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A275#3 | O275-4 | pSBX1K3-SspDnaXc*-sfGFP^C-H6 | 275 | Kan | DG7166 | #N/A | #N/A | ||||||
773 | 772 | Caro | 10-05 | 1096C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A275#3 | O275-5 | pSBX1K3-SspDnaXc*-sfGFP^C-H6 | 275 | Kan | DG7167 | #N/A | #N/A | ||||||
774 | 773 | Caro | 10-05 | 1096C4 | 25% glycerol in H2O | 3 | E. coli | Turbos | A275#3 | O275-7 | pSBX1K3-SspDnaXc*-sfGFP^C-H6 | 275 | Kan | #N/A | #N/A | |||||||
775 | 774 | Caro | 10-05 | 1125C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G312#2 | O312-4 | pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 | 312 | Cm | DG7168 | #N/A | #N/A | ||||||
776 | 775 | Caro | 10-05 | 1125C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G312#2 | O312-5 | pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 | 312 | Cm | #N/A | #N/A | |||||||
777 | 776 | Caro | 10-05 | 1125C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G312#2 | O312-6 | pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 | 312 | Cm | #N/A | #N/A | |||||||
778 | 777 | Caro | 10-05 | 1126C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G313#2 | O313-4 | pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 | 313 | Cm | DG7169 | ok, good | #N/A | #N/A | |||||
779 | 778 | Caro | 10-05 | 1126C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G313#2 | O313-5 | pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 | 313 | Cm | #N/A | #N/A | |||||||
780 | 779 | Caro | 10-05 | 1126C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G313#2 | O313-6 | pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 | 313 | Cm | #N/A | #N/A | |||||||
781 | 780 | Caro | 10-05 | 1127C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G314#2 | O314-4 | pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 | 314 | Cm | DG7170 | #N/A | #N/A | ||||||
782 | 781 | Caro | 10-05 | 1127C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G314#2 | O314-5 | pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 | 314 | Cm | DG7171 | #N/A | #N/A | ||||||
783 | 782 | Caro | 10-05 | 1127C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G314#2 | O314-6 | pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 | 314 | Cm | #N/A | #N/A | |||||||
784 | 783 | Caro | 10-05 | 1128C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G315#2 | O315-4 | pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 | 315 | Cm | DG7172 | ok, good | #N/A | #N/A | |||||
785 | 784 | Caro | 10-05 | 1128C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G315#2 | O315-5 | pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 | 315 | Cm | DG7173 | ok, but probably mutation in promoter, if possible use O315-4 | #N/A | #N/A | |||||
786 | 785 | Caro | 10-05 | 1128C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G315#2 | O315-6 | pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 | 315 | Cm | #N/A | #N/A | |||||||
787 | 786 | Jan | 10-05 | 1149C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S398 | O398 | pSB1C3-1GateNpuDnaE(N)*-H6 | 398 | Cm | #N/A | #N/A | |||||||
788 | 787 | Jan | 10-05 | 1150C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S399 | O399 | pSB1C3-1GateSspGyrB-S11C_H6 | 399 | Cm | #N/A | #N/A | |||||||
789 | 788 | Jan | 10-05 | 1151C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S400 | O400 | pSB1C3-H6-1GateNpu*(C)_v2 | 400 | Cm | #N/A | #N/A | |||||||
790 | 789 | Jan | 10-05 | 1152C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S401 | O401 | pSB1C3-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C) | 401 | Cm | #N/A | #N/A | |||||||
791 | 790 | Jan | 10-05 | 1153C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S402 | O402 | pSB1C3-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2 | 402 | Cm | #N/A | #N/A | |||||||
792 | 791 | Jan | 10-05 | 1154C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S403 | O403 | pSB1C3-T7RBS_BBv2 | 403 | Cm | #N/A | #N/A | |||||||
793 | 792 | Jan | 10-05 | 1154C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S403 | O403-2 | pSB1C3-T7RBS_BBv2 | 403 | Cm | #N/A | #N/A | |||||||
794 | 793 | Jan | 10-05 | 1155C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S284 | O284-3 | pSBX1K3-2Zv2Npu:LaLys+bad1 | 284 | Kan | #N/A | #N/A | |||||||
795 | 794 | MaxW | 10-06 | 1179C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S417 | O417 | pSB1C3-1GateGp41-1(N)-H6 | 417 | Cm | #N/A | #N/A | |||||||
796 | 795 | MaxW | 10-06 | 1181C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S418 | O418 | pSB1C3-H6-1GateGp41-1(C) | 418 | Cm | #N/A | #N/A | |||||||
797 | 796 | MaxW | 10-06 | 1183C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S419 | O419 | pSB1C3-DoubleGateGp41-1(N) | 419 | Cm | #N/A | #N/A | |||||||
798 | 797 | MaxW | 10-06 | 1185C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S420 | O420 | pSB1C3-DoubleGateGp41-1(C) | 420 | Cm | #N/A | #N/A | |||||||
799 | 798 | MaxW | 10-06 | 1187C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S421 | O421 | pSB1C3-DoubleNpuDnaE(N) | 421 | Cm | #N/A | #N/A | |||||||
800 | 799 | MaxW | 10-06 | 1189C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S422 | O422 | pSB1C3-DoubleGateNpuDnaE(C) | 422 | Cm | #N/A | #N/A | |||||||
801 | 800 | MaxW | 10-06 | 1190C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S404 | O404 | pSBX1A3-BBa_J04450 | 404 | Amp | #N/A | #N/A | |||||||
802 | 801 | MaxW | 10-06 | 1191C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S405 | O405 | pSBX1C3-BBa_J04450 | 405 | Cm | #N/A | #N/A | |||||||
803 | 802 | MaxW | 10-06 | 1192C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S406 | O406 | pSBX1K3-BBa_J04450 | 406 | Kan | #N/A | #N/A | |||||||
804 | 803 | MaxW | 10-06 | 1194C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S408 | O408 | pSBX4A5-BBa_J04450 | 408 | Amp | #N/A | #N/A | |||||||
805 | 804 | MaxW | 10-06 | 1195C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S409 | O409 | pSBX4C5-BBa_J04450 | 409 | Cm | #N/A | #N/A | |||||||
806 | 805 | Silvan | 10-07 | 954C1-3 | 25% glycerol in H2O | 7 | E. coli | Rosetta (DE3) | O232 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT), pRARE | 232 | 31 | Kan, Cm | #N/A | DD1384, DD1391 | ok, ok | ||||||
807 | 806 | Silvan | 10-07 | 955C1-2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O239-3 | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 | 239 | Kan | #N/A | DD1387, DD139428HE01 | no result, no resultok | |||||||
808 | 807 | Silvan | 10-07 | 978C1-3 | 25% glycerol in H2O | 7 | E. coli | Rosetta (DE3) | O250 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT), pRARE | 250 | 31 | Kan, Cm | #N/A | 28HD94 | ok | ||||||
809 | 808 | Silvan | 10-07 | 980C1-2 | 25% glycerol in H2O | 7 | E. coli | Rosetta (DE3) | O253 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6, pRARE | 253 | 31 | Kan, Cm | #N/A | 28HD83 | ok | ||||||
810 | 809 | Silvan | 10-07 | 1001C1-3 | 25% glycerol in H2O | 7 | E. coli | Rosetta (DE3) | O254-2 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT), pRARE | 254 | 31 | Kan, Cm | #N/A | 28HD93 | ok | ||||||
811 | 810 | Silvan | 10-07 | 1002C1-2 | 25% glycerol in H2O | 7 | E. coli | Rosetta (DE3) | O265-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6, pRARE | 265 | 31 | Kan, Cm | #N/A | 28HD92 | ok | ||||||
812 | 811 | Lisa | 10-07 | 1156C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S414 | O414 | pSB1C3-1GateSspDnaB(C*) | 414 | Cm | DG7142, IC5216, IC5221 | bad, SspDnaEN, SspDnaEN | #N/A | #N/A | |||||
813 | 812 | Lisa | 10-07 | 1157C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S414#2 | O414-2 | pSB1C3-1GateSspDnaB(C*) | 414 | Cm | DG7143 | #N/A | #N/A | ||||||
814 | 813 | Lisa | 10-07 | 1158C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S414#3 | O414-3 | pSB1C3-1GateSspDnaB(C*) | 414 | Cm | DG7144 | #N/A | #N/A | ||||||
815 | 814 | Lisa | 10-07 | 1159C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S414#4 | O414-4 | pSB1C3-1GateSspDnaB(C*) | 414 | Cm | DG7145 | #N/A | #N/A | ||||||
816 | 815 | Lisa | 10-07 | 1160C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S415 | O415 | pSB1C3-1GateSspDnaB(N) | 415 | Cm | DG7146, IC5213, IC5218 | bad, actually SspDnaEC, actually SspDnaEC | #N/A | #N/A | |||||
817 | 816 | Lisa | 10-07 | 1161C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S415#2 | O415-2 | pSB1C3-1GateSspDnaB(N) | 415 | Cm | DG7147 | bad | #N/A | #N/A | |||||
818 | 817 | Lisa | 10-07 | 1162C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S415#3 | O415-3 | pSB1C3-1GateSspDnaB(N) | 415 | Cm | DG7148 | bad | #N/A | #N/A | |||||
819 | 818 | Lisa | 10-07 | 1163C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S415#4 | O415-4 | pSB1C3-1GateSspDnaB(N) | 415 | Cm | DG7149 | bad | #N/A | #N/A | |||||
820 | 819 | Lisa | 10-07 | 1164C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S416 | O416 | pSB1C3-1GateSspDnaB(N*) | 416 | Cm | DG7150, IC5215, IC5220 | bad, actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie, actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie | #N/A | #N/A | |||||
821 | 820 | Lisa | 10-07 | 1166C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S411 | O411 | pSB1C3-1GateSspDnaE(N) | 411 | Cm | DG7152 | #N/A | #N/A | ||||||
822 | 821 | Lisa | 10-07 | 1167C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S411#2 | O411-2 | pSB1C3-1GateSspDnaE(N) | 411 | Cm | DG7153 | #N/A | #N/A | ||||||
823 | 822 | Lisa | 10-07 | 1168C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S411#3 | O411-3 | pSB1C3-1GateSspDnaE(N) | 411 | Cm | DG7154 | #N/A | #N/A | ||||||
824 | 823 | Lisa | 10-07 | 1169C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S411#4 | O411-4 | pSB1C3-1GateSspDnaE(N) | 411 | Cm | DG7155, IC5217, IC5222 | , actually SspDnaBC*, actually SspDnaBC* | #N/A | #N/A | |||||
825 | 824 | Lisa | 10-07 | 1165C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S416#2 | O416-2 | pSB1C3-1GateSspDnaB(N*) | 416 | Cm | DG7151 | not mixed up, missing? | #N/A | #N/A | |||||
826 | 825 | Lisa | 10-07 | 1170C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S412 | O412 | pSB1C3-1GateSspDnaE(C) | 412 | Cm | DG7156 | #N/A | #N/A | ||||||
827 | 826 | Lisa | 10-07 | 1171C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S412#2 | O412-2 | pSB1C3-1GateSspDnaE(C) | 412 | Cm | DG7157, IC5260 | , actually sspdnaBN | #N/A | #N/A | |||||
828 | 827 | Lisa | 10-07 | 1172C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S412#3 | O412-3 | pSB1C3-1GateSspDnaE(C) | 412 | Cm | DG7158, IC5261 | , actually sspdnaBN | #N/A | #N/A | |||||
829 | 828 | Lisa | 10-07 | 1173C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S412#4 | O412-4 | pSB1C3-1GateSspDnaE(C) | 412 | Cm | DG7159 | #N/A | #N/A | ||||||
830 | 829 | Lisa | 10-07 | 1175C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S413#2 | O413 | pSB1C3-1GateSspDnaB(C) | 413 | Cm | DG7160 | #N/A | #N/A | ||||||
831 | 830 | Lisa | 10-07 | 1175C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S413#2 | O413-2 | pSB1C3-1GateSspDnaB(C) | 413 | Cm | DG7161, IC5214, IC5219 | bad, actually SspDnaBN*, actually SspDnaBN* | #N/A | #N/A | |||||
832 | 831 | Caro | 10-07 | 1205C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S386#3 | O386 | pSBX4C5-0GateTer*-coex_mRFP168/169 | 386 | Cm | IC5185, IC5186 | good, ok, good, ok | #N/A | #N/A | |||||
833 | 832 | Caro | 10-07 | 1207C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S387#2 | O387 | pSBX4C5-0GateNpu*-coex_mRFP168/169 | 387 | Cm | IC5187, IC5188 | good, ok, good, ok | #N/A | #N/A | |||||
834 | 833 | Caro | 10-07 | 1208C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S387#3 | O387-2 | pSBX4C5-0GateNpu*-coex_mRFP168/169 | 387 | Cm | #N/A | #N/A | |||||||
835 | 834 | Caro | 10-07 | 1201C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S384 | O384 | pSBX4C5-0GateDnaX*-coex_mRFP168/169 | 384 | Cm | IC5189, IC5190 | no DnaXN, no DNnaXN | #N/A | #N/A | |||||
836 | 835 | Caro | 10-07 | 1203C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S386 | O386-2 | pSBX4C5-0GateTer*-coex_mRFP168/169 | 386 | Cm | #N/A | #N/A | |||||||
837 | 836 | Caro | 10-07 | 1204C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S386#2 | O386-3 | pSBX4C5-0GateTer*-coex_mRFP168/169 | 386 | Cm | #N/A | #N/A | |||||||
838 | 837 | Caro | 10-07 | 1209C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S397 | O397 | pSBX1K3-DnaX-mRFP168/169_coex | 397 | Kan | IC5191, IC5192 | good, ok, good, ok | #N/A | #N/A | |||||
839 | 838 | Caro | 10-07 | 1198C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S255#3 | O255-3 | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 | 255 | Cm | IC5193, IC5194 | good, ok, good, ok | #N/A | #N/A | |||||
840 | 839 | Caro | 10-07 | 1198C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | S255#3 | O255-4 | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 | 255 | Cm | #N/A | #N/A | |||||||
841 | 840 | Caro | 10-07 | 1199C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S256#2 | O256 | pSBX4C5-0GateSspGyrB-coex_mRFP168/169 | 256 | Cm | IC5195, IC5196 | good, ok, good, ok | #N/A | #N/A | |||||
842 | 841 | Caro | 10-07 | 1200C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S257#3 | O257-3 | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 | 257 | Cm | IC5197, IC5198 | mutations after Biobrick, good ok, mutations after Biobrick, good ok | #N/A | #N/A | |||||
843 | 842 | Caro | 10-07 | 1213C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S393 | O393 | pSBX1K3-H6-LOV-BsaIIasB-mRFP_coex | 393 | Kan | IC5199, IC5200 | ok, good, ok, good | #N/A | #N/A | |||||
844 | 843 | Caro | 10-07 | 1212C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S396 | O396 | pSBX1K3-DnaX-mRFP*168/169_coex | 396 | Kan | IC5201, IC5202 | ok, good, ok, good | #N/A | #N/A | |||||
845 | 844 | Caro | 10-07 | 1212C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S396 | O396-2 | pSBX1K3-DnaX-mRFP*168/169_coex | 396 | Kan | #N/A | #N/A | |||||||
846 | 845 | Caro | 10-07 | 1211C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S397#3 | O397-2 | pSBX1K3-DnaX-mRFP168/169_coex | 397 | Kan | #N/A | #N/A | |||||||
847 | 846 | Caro | 10-07 | 1210C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S397#2 | O397-3 | pSBX1K3-DnaX-mRFP168/169_coex | 397 | Kan | #N/A | #N/A | |||||||
848 | 847 | Caro | 10-07 | 1215C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S395 | O395 | pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex | 395 | Kan | IC5203, IC5204 | ok, good, probably frameshift after CDS, ok, good, probably frameshift after CDS | #N/A | #N/A | |||||
849 | 848 | Caro | 10-07 | 1216C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S395#2 | O395-2 | pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex | 395 | Kan | #N/A | #N/A | |||||||
850 | 849 | Caro | 10-07 | 1217C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S395#3 | O395-3 | pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex | 395 | Kan | #N/A | #N/A | |||||||
851 | 850 | Caro | 10-07 | 1218C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S391 | O391 | pSBX1K3-DnaX-sfGFP_coex | 391 | Kan | IC5205, IC5206 | ok, good, ok,good | #N/A | #N/A | |||||
852 | 851 | Caro | 10-07 | 1219C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S392 | O392 | pSBX1K3-DnaX*-sfGFP_coex | 392 | Kan | IC5207, IC5208 | ok, good, ok,good | #N/A | #N/A | |||||
853 | 852 | Caro | 10-07 | 1220C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | S392#2 | O392-2 | pSBX1K3-DnaX*-sfGFP_coex | 392 | Kan | #N/A | #N/A | |||||||
854 | 853 | Caro | 10-07 | 1221C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S392#3 | O392-3 | pSBX1K3-DnaX*-sfGFP_coex | 392 | Kan | #N/A | #N/A | |||||||
855 | 854 | Caro | 10-07 | 1222C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S388 | O388 | pSBX1K3-LOV-BsaIIasB-sfGFP_coex | 388 | Kan | IC5209, IC5210 | ok, good, ok,good | #N/A | #N/A | |||||
856 | 855 | Caro | 10-07 | 1223C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S389 | O389 | pSBX1K3-LOV-12-DnaXC-sfGFP_coex | 389 | Kan | IC5211, IC5212 | ok, good, ok,good | #N/A | #N/A | |||||
857 | 856 | Silvan | 10-07 | 66C1-17 | 25% glycerol in H2O | 7 | E. coli | Rosetta (DE3) | pRARE | 31 | Cm | #N/A | ||||||||||
858 | 857 | Silvan | 10-07 | 1226C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | G235 | pSB1C3-Smt3-2ZSortA-H6 (mRFP) | 235 | Cm | #N/A | #N/A | #N/A | |||||||
859 | 858 | Silvan | 10-07 | 1226C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | G235 | O235 | pSB1C3-Smt3-2ZSortA-H6 (mRFP) | 235 | Cm | IC5225, IC5231 | C->A point mutation in mRFP barcode, mixture of mRFP and ccdB | #N/A | #N/A | |||||
860 | 859 | Silvan | 10-07 | 1226C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | G235 | O235-2 | pSB1C3-Smt3-2ZSortA-H6 (mRFP) | 235 | Cm | IC5226, | C->A point mutation in mRFP barcode, | #N/A | #N/A | |||||
861 | 860 | Silvan | 10-07 | 1228C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S234 | pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 234 | Cm | #N/A | #N/A | #N/A | |||||||
862 | 861 | Silvan | 10-07 | 1228C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S234 | O234 | pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 234 | Cm | #N/A | #N/A | |||||||
863 | 862 | Silvan | 10-07 | 1228C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | S234 | O234-2 | pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 234 | Cm | #N/A | #N/A | |||||||
864 | 863 | Silvan | 10-07 | 1229C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S423 | O423 | pSB1C3-T7RBS-H6-Smt3-0ZSortA | 423 | Cm | #N/A | #N/A | |||||||
865 | 864 | Silvan | 10-07 | 1229C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S423 | O423-2 | pSB1C3-T7RBS-H6-Smt3-0ZSortA | 423 | Cm | #N/A | #N/A | |||||||
866 | 865 | Silvan | 10-07 | 1229C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | S423 | O423-3 | pSB1C3-T7RBS-H6-Smt3-0ZSortA | 423 | Cm | #N/A | #N/A | |||||||
867 | 866 | Silvan | 10-07 | 1230C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | S425 | O425 | pSB1C3-T7RBS-H6-0ZSortA | 425 | Cm | #N/A | #N/A | |||||||
868 | 867 | Silvan | 10-07 | 1230C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | S425 | O425-2 | pSB1C3-T7RBS-H6-0ZSortA | 425 | Cm | #N/A | #N/A | |||||||
869 | 868 | Silvan | 10-07 | 1230C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | S425 | O425-3 | pSB1C3-T7RBS-H6-0ZSortA | 425 | Cm | #N/A | #N/A | |||||||
870 | 869 | Silvan | 10-07 | 1231C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A177#4 | O177-4 | pSB1C3-2ZSortA-H6 | 177 | Cm | IC5227, IC5232 | ok, ok | #N/A | #N/A | |||||
871 | 870 | Silvan | 10-07 | 1231C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A177#4 | O177-5 | pSB1C3-2ZSortA-H6 | 177 | Cm | IC5228, IC5233 | ok, ok | #N/A | #N/A | |||||
872 | 871 | Silvan | 10-07 | 1231C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A177#4 | O177-6 | pSB1C3-2ZSortA-H6 | 177 | Cm | #N/A | #N/A | |||||||
873 | 872 | Silvan | 10-07 | 1232C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | A426 | O426 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 426 | Cm | IC5229, IC5234, IC5236, DD1482 | is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP, is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP, no result, is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP | #N/A | #N/A | |||||
874 | 873 | Silvan | 10-07 | 1232C2 | 25% glycerol in H2O | 3 | E. coli | Turbos | A426 | O426-2 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 426 | Cm | IC5230, IC5235, DD1481, DD1483 | ok, ok, ok, ok | #N/A | #N/A | |||||
875 | 874 | Silvan | 10-07 | 1232C3 | 25% glycerol in H2O | 3 | E. coli | Turbos | A426 | O426-3 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 426 | Cm | #N/A | #N/A | |||||||
876 | 875 | Caro | 10-10 | 1235C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O389 | pSBX1K3-LOV-12-DnaXC-sfGFP_coex | 389 | Kan | #N/A | IC5211, IC5212 | ok, good, ok,good | |||||||
877 | 876 | Caro | 10-10 | 1236C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O392 | pSBX1K3-DnaX*-sfGFP_coex | 392 | Kan | #N/A | IC5207, IC5208 | ok, good, ok,good | |||||||
878 | 877 | Caro | 10-10 | 1237C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O391 | pSBX1K3-DnaX-sfGFP_coex | 391 | Kan | #N/A | IC5205, IC5206 | ok, good, ok,good | |||||||
879 | 878 | Caro | 10-10 | 1238C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O278 | pSBX1K3-DnaXN-sfGFPn-H6 | 278 | Kan | #N/A | DD1413 | ok one additional His | |||||||
880 | 879 | Caro | 10-10 | 1239C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O276 | pSBX1K3-SspDnaXc-sfGFP^C-H6 | 276 | Kan | #N/A | DD1417 | ok? two additional His | |||||||
881 | 880 | Caro | 10-10 | 1240C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O395 | pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex | 395 | Kan | #N/A | IC5203, IC5204 | ok, good, probably frameshift after CDS, ok, good, probably frameshift after CDS | |||||||
882 | 881 | Caro | 10-10 | 1241C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O396 | pSBX1K3-DnaX-mRFP*168/169_coex | 396 | Kan | #N/A | IC5201, IC5202 | ok, good, ok, good | |||||||
883 | 882 | Caro | 10-10 | 1242C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O397 | pSBX1K3-DnaX-mRFP168/169_coex | 397 | Kan | #N/A | IC5191, IC5192 | good, ok, good, ok | |||||||
884 | 883 | Caro | 10-10 | 1243C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O272-4 | pSBX1K3-H6-DnaXC-mRFPc-H6 | 272 | Kan | #N/A | DG7162 | ||||||||
885 | 884 | Caro | 10-10 | 1244C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O267-3 | pSBX1K3-H6-mRFPn-DnaXN-H6 | 267 | Kan | #N/A | 28HE15 | ok, good | |||||||
886 | 885 | Caro | 10-10 | 1245C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O260-5 | O260-6 | pSBX1K3-BBa_I13507-GSGIDECHT | 260 | Kan | DD2690, DD2691 | , | |||||||
887 | 886 | Caro | 10-10 | 1245C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O260-5 | O260-7 | pSBX1K3-BBa_I13507-GSGIDECHT | 260 | Kan | DD2692, DD2693 | , | |||||||
888 | 887 | Caro | 10-10 | 1245C3 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O260-5 | pSBX1K3-BBa_I13507-GSGIDECHT | 260 | Kan | #N/A | |||||||||
889 | 888 | Caro | 10-10 | 1247C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O59-14 | pSBX1K3-BBa_I13507 | 59 | Kan | #N/A | |||||||||
890 | 889 | Caro | 10-10 | 1248C1 | 25% glycerol in H2O | 7 | E. coli | Rosetta (DE3) | O271-3 | 271 | 31,29 | #N/A | #N/A | DG7164 | ||||||||
891 | 890 | Caro | 10-10 | 1246C1 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O261-3 | O261-4 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 261 | Kan | DD2694, DD2695 | , | |||||||
892 | 891 | Caro | 10-10 | 1246C2 | 25% glycerol in H2O | 6 | E. coli | BI21(DE3) | O261-3 | O261-5 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 261 | Kan | DD2696, DD2697 | , | |||||||
893 | 892 | Caro | 10-10 | 1256C1 | 25% glycerol in H2O | 3 | E. coli | Turbos | O261-3 | O261-6 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 261 | Kan | DD2698, DD2699 | , | |||||||
894 | 893 | 10-13 | 1257C1 | 25% glycerol in H2O | #N/A | #N/A | #N/A | O394 | #N/A | #N/A | #N/A | #N/A | #N/A | DD2689, DD2688 | ok, good, ok, good | |||||||
895 | 894 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
896 | 895 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
897 | 896 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
898 | 897 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
899 | 898 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
900 | 899 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
901 | 900 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
902 | 901 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
903 | 902 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
904 | 903 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
905 | 904 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
906 | 905 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
907 | 906 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
908 | 907 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
909 | 908 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
910 | 909 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
911 | 910 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
912 | 911 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
913 | 912 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
914 | 913 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
915 | 914 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
916 | 915 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
917 | 916 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
918 | 917 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
919 | 918 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
920 | 919 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
921 | 920 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
922 | 921 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
923 | 922 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
924 | 923 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
925 | 924 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
926 | 925 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
927 | 926 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
928 | 927 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
929 | 928 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
930 | 929 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
931 | 930 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
932 | 931 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
933 | 932 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
934 | 933 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
935 | 934 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
936 | 935 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
937 | 936 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
938 | 937 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
939 | 938 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
940 | 939 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
941 | 940 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
942 | 941 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
943 | 942 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
944 | 943 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
945 | 944 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
946 | 945 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
947 | 946 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
948 | 947 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
949 | 948 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
950 | 949 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
951 | 950 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
952 | 951 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
953 | 952 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
954 | 953 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
955 | 954 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
956 | 955 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
957 | 956 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
958 | 957 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
959 | 958 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
960 | 959 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
961 | 960 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
962 | 961 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
963 | 962 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
964 | 963 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
965 | 964 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
966 | 965 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
967 | 966 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
968 | 967 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
969 | 968 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
970 | 969 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
971 | 970 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
972 | 971 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
973 | 972 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
974 | 973 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
975 | 974 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
976 | 975 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
977 | 976 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
978 | 977 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
979 | 978 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
980 | 979 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
981 | 980 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
982 | 981 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
983 | 982 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
984 | 983 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
985 | 984 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id | ||||||||||||
986 | 985 | 25% glycerol in H2O | < insert growth id | #N/A | #N/A | < insert growth id | < insert growth id | < insert growth id | < insert growth id | < insert growth id |