Team:Heidelberg/AwesomeSheet/Samples

From 2014.igem.org

– Samples

ABCDEFGHIJKLMNO
1
NameExperimantorDate takenTime takenoriginal OD600Related GrowthRelated InductionDescriptionPOIPOI 2POI 3
2
NameMWNameMWNameMW
3
kDakDakDa
4
276uJan06-0814:361.08356c1-223Uninduced
5
286:1 1/2Jan06-0817:521.96356c1-223
1.5 h after induction with 0.4 mM IPTG
H6-GB1-NpuDnaEN20.4H6-GB18.5NpuDnaEN11.9
6
296:3hJan06-0819:232.7356c1-2233 h after induction with 0.4 mM IPTGH6-GB1-NpuDnaEN20.4H6-GB18.5NpuDnaEN11.9
7
306:ONJan06-0810:0011356c1-223
16 h after induction with 0.4 mM IPTG
H6-GB1-NpuDnaEN20.4H6-GB18.5NpuDnaEN11.9
8
317uJan06-0814:360.96357c1-223Uninduced
9
327:1 1/2Jan06-0817:301.6357c1-223
1.5 h after induction with 0.4 mM IPTG
H6-NpuDnaEC-IgG15.5
H6-NpuDnaEC
6.3IgG9.3
10
337:3hJan06-0818:552.4357c1-2233 h after induction with 0.4 mM IPTGH6-NpuDnaEC-IgG15.5
H6-NpuDnaEC
6.3IgG9.3
11
347:ONJan06-0810:0010.4357c1-223
16 h after induction with 0.4 mM IPTG
H6-NpuDnaEC-IgG15.5
H6-NpuDnaEC
6.3IgG9.3
12
358uJan06-0814:361.03358c1-223Uninduced
13
368:1 1/2Jan06-0819:232.1358c1-223
1.5 h after induction with 0.4 mM IPTG
Strep-NpuDnaEC-eGFP-H6
14
378:3hJan06-0820:503.27358c1-2233 h after induction with 0.4 mM IPTG
15
388:ONJan06-0810:002.15358c1-223
16 h after induction with 0.4 mM IPTG
16
399uJan06-0814:360.96359c1-223Uninduced
17
409:1 1/2Jan06-0817:521.88359c1-223
1.5 h after induction with 0.4 mM IPTG
18
419:3hJan06-0819:232.79359c1-2233 h after induction with 0.4 mM IPTG
19
429:ONJan06-0810:004.7359c1-223
16 h after induction with 0.4 mM IPTG
20
4310uJan06-0814:361.61360c1-223Uninduced
21
4410:1 1/2Jan06-0817:522.44360c1-223
1.5 h after induction with 0.4 mM IPTG
22
4510:3hJan06-0819:233.42360c1-2233 h after induction with 0.4 mM IPTG
23
4610:ONJan06-0810:006360c1-223
16 h after induction with 0.4 mM IPTG
24
4711uJan06-0814:361.32361c1-223Uninduced
25
4811:1 1/2Jan06-0817:302.3361c1-223
1.5 h after induction with 0.4 mM IPTG
26
4911:3hJan06-0818:553.15361c1-2233 h after induction with 0.4 mM IPTG
27
5011:ONJan06-0810:006361c1-223
16 h after induction with 0.4 mM IPTG
28
51pJJDuet30, pSFBAD094.4353C119
29
52pJJDuet3012.4354c121
30
53pSFBAD093.2355c121
31
54pSB1AK8-BBa_I712074-2ZSspH6:GFP23.4372c125
32
55pSB1AK8-BBa_I712074-2ZSspH6:GFP30.4352c118
33
56pSBX4C5-2ZSspH6:GFP18299c1
34
577.4300c1
35
5810.2351c118
36
59GFP-His control16.6
37
60Inc GFP #210.4
38
61Silvan 6628.8
39
62AK Npu Silvan263C1-32837°CGFP27.7
40
63pSB1AK8-BBa_I712074-2ZSspH6:GFP372C22837°CGFP27.7
41
64pSB1AK8-BBa_I712074-2ZSspH6:GFP372C32828°CGFP27.7
42
65pSBX4C5-2ZSspH6:GFP300C22837°CGFP27.7
43
66pSBX4C5-2ZSspH6:GFP300C32828°CGFP27.7
44
67C+Jan08-27536C1-139
3ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 50% power on ice, centrifuged at 18000 rcf for 10 min, supernatant
H6-NpuDnaE^C-sfGFP^C-H625.7
H6-NpuDnaE^C
20.7
sfGFP^C-H6
5
45
68C*+Jan08-27537C1-139as sample 67
H6-NpuDnaE^C SN->AG-sfGFP^C-H6
25.7
H6-NpuDnaE^C*
20.7
sfGFP^C-H6
5
46
69N+Jan08-27538C1-139as sample 67H6-sfGFP^N-NpuDnaE^N-H620.4
NpuDnaE^N-H6
12.7
H6-sfGFP^N
7.7
47
70N*+Jan08-27539C1-139as sample 67
H6-sfGFP^N-C->G NpuDnaE^N-H6
20.4
NpuDnaE^N*-H6
12.7
H6-sfGFP^N*
7.7
48
71sfGFP+Jan08-27540C1-139as sample 67, FCS: ~1µMH6-sfGFP-H628.5
49
72sfGFP+Jan08-27540C2-139as sample 67H6-sfGFP-H628.5
50
73C+ PelletJan08-27536C1-139
3ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 50% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
H6-NpuDnaE^C-sfGFP^C-H625.7
H6-NpuDnaE^C
20.7
sfGFP^C-H6
5
51
74C*+ PelletJan08-27537C1-139as sample 73
H6-NpuDnaE^C SN->AG-sfGFP^C-H6
25.7
H6-NpuDnaE^C*
20.7
sfGFP^C-H6
5
52
75N+ PelletJan08-27538C1-139as sample 73H6-sfGFP^N-NpuDnaE^N-H620.4
NpuDnaE^N-H6
12.7
H6-sfGFP^N
7.7
53
76N*+ PelletJan08-27539C1-139as sample 73
H6-sfGFP^N-C->G NpuDnaE^N-H6
20.4
NpuDnaE^N*-H6
12.7
H6-sfGFP^N*
7.7
54
77sfGFP+ PelletJan08-27540C1-139as sample 73H6-sfGFP-H628.5
55
78sfGFP+ PelletJan08-27540C2-139as sample 73H6-sfGFP-H628.5
56
79C-Jan08-27536C15 ml, as sample 67H6-NpuDnaE^C-sfGFP^C-H625.7
H6-NpuDnaE^C
20.7
sfGFP^C-H6
5
57
80C*-Jan08-27537C15 ml, as sample 67
H6-NpuDnaE^C SN->AG-sfGFP^C-H6
25.7
H6-NpuDnaE^C*
20.7
sfGFP^C-H6
5
58
81N-Jan08-27538C15 ml, as sample 67H6-sfGFP^N-NpuDnaE^N-H620.4
NpuDnaE^N-H6
12.7
H6-sfGFP^N
7.7
59
82N*-Jan08-27539C15 ml, as sample 67
H6-sfGFP^N-C->G NpuDnaE^N-H6
20.4
NpuDnaE^N*-H6
12.7
H6-sfGFP^N*
7.7
60
83sfGFP-Jan08-27540C15 ml, as sample 67H6-sfGFP-H628.5
61
84sfGFP-Jan08-27540C25 ml, as sample 67H6-sfGFP-H628.5
62
85C- PelletJan08-27536C15 ml, as sample 67H6-NpuDnaE^C-sfGFP^C-H625.7
H6-NpuDnaE^C
20.7
sfGFP^C-H6
5
63
86C*- PelletJan08-27537C15 ml, as sample73
H6-NpuDnaE^C SN->AG-sfGFP^C-H6
25.7
H6-NpuDnaE^C*
20.7
sfGFP^C-H6
5
64
87N- PelletJan08-27538C15 ml, as sample73H6-sfGFP^N-NpuDnaE^N-H620.4
NpuDnaE^N-H6
12.7
H6-sfGFP^N
7.7
65
88N*- PelletJan08-27539C15 ml, as sample73
H6-sfGFP^N-C->G NpuDnaE^N-H6
20.4
NpuDnaE^N*-H6
12.7
H6-sfGFP^N*
7.7
66
89sfGFP- PelletJan08-27540C15 ml, as sample73H6-sfGFP-H628.5
67
90sfGFP- PelletJan08-27540C25 ml, as sample73H6-sfGFP-H628.5
68
91C_SNCaro08-30536C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
69
92C*_SNCaro08-30537C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
70
93N_SNCaro08-30538C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
71
94N*_SNCaro08-30539C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
72
95CN_invivo__SNCaro08-30552C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
73
96sfGFP_SNCaro08-30540C2-4
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
74
97C_PelletCaro08-30536C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
75
98C*_PelletCaro08-30537C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
76
99N_PelletCaro08-30538C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
77
100N*_PelletCaro08-30539C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
78
101CN_invivo__PelletCaro08-30552C2-2
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
79
102sfGFP_PelletCaro08-30540C2-4
20ml, harvested by centrifugation for 5 min at 3270rcf and 4°C, resuspendended in 500 µL PBS on ice, sonificated for 2 min at 60% power on ice, centrifuged at 18000 rcf for 10 min, pellet resuspended in 500 mL PBS
80
103CNJan08-29552C340as sample 67H6-sfGFP-H628.5
H6-NpuDnaE^C
20.77
NpuDnaE^N-H6
12.7
81
104CN PelletJan08-29552C340as sample 73H6-sfGFP-H628.5
H6-NpuDnaE^C
20.77
NpuDnaE^N-H6
12.7
82
105C+NJan08-28Mixed Sample 67 and 69
83
106BI21DE3Charlotte8/26/201426C1-9BI21DE3: background control
84
107pSB1AK8-BBa_I712074-2ZSspH6:GFPCharlotte8/24/2014510C2
85
108pSB1C3-BBa_I746907Charlotte09-02591C2GFP control
86
109pSB1AK8-T7-2ZNpu:(GFP-H6)Charlotte09-02263C1-7
87
110pSB1AK8-BBa_I712074-2ZSspH6:GFPCharlotte09-02510C2-2
88
111BI21(DE3)Charlotte09-026C1-10
89
112pSB1C3-BBa_I746907Charlotte09-02591C2Pellet
90
113pSB1AK8-T7-2ZNpu:(GFP-H6)Charlotte09-02263C1-7PelletGFP-H6_circ
91
114pSB1AK8-BBa_I712074-2ZSspH6:GFPCharlotte09-02510C2-2Pellet
92
115BI21(DE3)Charlotte09-026C1-10Pellet
93
116C+N SNCaro30-8Mixed Sample 91 and 93
94
117C*+N* SNCaro30-8Mixed Sample 92 and 94
95
118C+N PelletCaro30-8Mixed Sample 97 and 99
96
119C*+N* PelletCaro30-8Mixed Sample 98 and 100
97
120pSBX1K3-2Zv2Npu:(LambdaLys-H6)Jan09-185:392.64597C1-854
took 2ODml from Liquid culture, spun down at 18000rcf for 5 min removed supernatant stored at -55°C
98
121pSBX1K3-LiLaLy(H6)Charlotte09-19733C2
took 2ODml from Liquid culture, spun down at 18000rcf for 5 min removed supernatant stored at -55°C
99
122Bl21(DE3)Charlotte09-226C1-14background control
100
158Lin. XylanaseCharlotte09-24840C1-3
101
159Circ. XylanaseCharlotte09-24611C1-2
102
160Lin. Xylanase + HisCharlotte09-24841C1-3
103
161Circ. Xylanas + HisCharlotte09-24563C1-2
104
162Lin. Xylanase + His + Ext.Charlotte09-24923C1
105
163Circ (destr. Ext.) Xylanase + HisCharlotte09-24598C1-2
106
164BL21-6Charlotte09-246C1-16
107
165Circ Xylanase. His (2)Charlotte09-24611C1-2
108
166Lin Xylanase (2)Charlotte09-24841C1-3
109
167pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3Charlotte09-25436C1-11
110
168pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)Charlotte09-25596C1-7
111
169pSBX1K3-2Zv2Npu:(LambdaLys-H6)Charlotte09-25597C1-9
112
170pSBX1K3-LiLaLy(H6)Charlotte09-25733C2-2
113
Dnmt1 #5
pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6, pRARE
Silvan09-30828C1-3Dnmt1_Sort w/o linker
114
Dnmt1 #6
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT), pRARE
Silvan09-30978C1-1/2Dnmt1_Intein_rigid linker
115
Dnmt1 #7
pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT), pRARE
Silvan09-301001C1-1/2Dnmt1_Intein_flex linker
116
Dnmt1 #8Silvan09-301002C1-1/2Dnmt1_Sort late rigid linker
117
175360 - BL21 ctrlJakob09-30
118
176360 - NJakob09-30
119
177360 - CJakob09-30
120
178360 - N*Jakob09-30
121
179360 - C*Jakob09-30
122
180360 - NCJakob09-30
123
181360 - N*C*Jakob09-30
124
182360 - sfGFP*Jakob09-30
125
183360 - sfGFPJakob09-30
126
184000 - NCJakob09-30
127
185000 - N*C*Jakob09-30
128
186030 - NCJakob09-30
129
187030 - N*C*Jakob09-30
130
188060 - NCJakob09-30
131
189060 - N*C*Jakob09-30
132
190120 - NCJakob09-30
133
191120 - N*C*Jakob09-30
134
192180 - NCJakob09-30
135
193180 - N*C*Jakob09-30
136
194240 - NCJakob09-30
137
195240 - N*C*Jakob09-30
138
196360 - NCJakob09-30
139
197360 - N*C*Jakob09-30
140
198480 - NCJakob09-30
141
199480 - N*C*Jakob09-30
142
200600 - NCJakob09-30
143
201600 - N*C*Jakob09-30
144
202Xyla Lin + His (purified)Consti10-03
145
203Xyla Circ + His (purified)Consti10-03
146
204pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3Charlotte10-031044C1-2
147
205pSBX1K3-2Zv2Npu:LaLys+may1Charlotte10-031046C1-2
148
206pSBX1K3-2Zv2Npu:LaLys+sgt2Charlotte10-031047C1-2
149
207pSBX1K3-2Zv2Npu:LaLys+sho1Charlotte10-031048C1-2
150
208pSBX1K3-2Zv2Npu:LaLys+sho2Charlotte10-031049C1-3
151
209pSBX1K3-2Zv2Npu:LaLys+flexlinkerCharlotte10-03436C1-13
152
210pSBX1K3-2Zv2Npu:LaLys+ord1Charlotte10-031114C1
153
211pSBX1K3-2Zv2Npu:LaLys+ord3Charlotte10-031115C1
154
212pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3Charlotte10-03436C1-14
155
213pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)Charlotte10-03596C1-8
156
214pSBX1K3-2Zv2Npu:(LambdaLys-H6)Charlotte10-03597C1-10
157
215pSBX1K3-2Zv2Npu:LaLys+may1Charlotte10-031044C1-3
158
216pSBX1K3-2Zv2Npu:LaLys+sgt2Charlotte10-031046C1-3
159
217pSBX1K3-2Zv2Npu:LaLys+sho1Charlotte10-031047C1-3
160
218pSBX1K3-2Zv2Npu:LaLys+sgt4Charlotte10-031018C1-1
161
219pSBX1K3-2Zv2Npu:LaLys+flexlinkerCharlotte10-031049C1-4
162
220pSBX1K3-2Zv2Npu:LaLys+ord1Charlotte10-031114C1-2
163
221pSBX1K3-2Zv2Npu:LaLys+ord3Charlotte10-031115C1-2
164
222
165
223
166
224
167
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