Team:Heidelberg/AwesomeSheet/SURPREMErun
From 2014.igem.org
Lab book
– SURPREMErun
A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | AA | ||
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1 | Barcode | Datum | Tube | H2O | Vtotal/µl | Primer | Result | Analyser | Costs: | 2650.5 | Red length | HQ | Sequence | |||||||||||||||
2 | WatchBox | ID | Name | c/(ng/µl) | V/µL | V/µl | Barcode | Name | Name | 0 | bp | 0% | ||||||||||||||||
3 | BF8244 | 06-10 | O1 | BBa_J61002-BBa_J23110 | 60 | 4.96 | 15.04 | 20 | 146694 | VF2 | ok | tgccacctgacgtctaagaa | ||||||||||||||||
4 | BF8245 | 06-10 | O2 | BBa_J61002-BBa_J23118 | 60 | 2.86 | 17.14 | 20 | 146694 | VF2 | ok | tgccacctgacgtctaagaa | ||||||||||||||||
5 | BF8246 | 06-10 | O3 | BBa_J61002-BBa_J23100 | 60 | 5.02 | 14.98 | 20 | 146694 | VF2 | ok | tgccacctgacgtctaagaa | ||||||||||||||||
6 | BF8247 | 06-10 | O7 | pSB1C3-BBa_I15016 | 60 | 8.28 | 11.72 | 20 | 146694 | VF2 | was previously thought to be BBa_C0012 | tgccacctgacgtctaagaa | ||||||||||||||||
7 | BF8248 | 06-10 | O5 | pSB1C3-BBa_E0240 | 60 | 4.84 | 15.16 | 20 | 146694 | VF2 | insertion before prefix leading to additional EcoRI site | tgccacctgacgtctaagaa | ||||||||||||||||
8 | BF8249 | 06-10 | O6 | pSB1C3-BBa_R0010 | 60 | 10.08 | 9.92 | 20 | 146694 | VF2 | ok | tgccacctgacgtctaagaa | ||||||||||||||||
9 | BF8250 | 06-10 | O4 | pSB1C3-BBa_C0012 | 60 | 6.49 | 13.51 | 20 | 146694 | VF2 | was prevoiously thought to be BBa_I15016 | tgccacctgacgtctaagaa | ||||||||||||||||
10 | BF8251 | 06-10 | O8 | pSB1C3-BBa_I15017 | 60 | 6.22 | 13.78 | 20 | 146694 | VF2 | ok | tgccacctgacgtctaagaa | ||||||||||||||||
11 | 146694 | 06-10 | VF2 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | 200/100 | tgccacctgacgtctaagaa | td> | #N/A | |||||||||||||||
12 | BF8252 | 06-20 | O13 | pSB1C3-pKH6insert | 51 | 10.00 | 10.00 | 20 | 146695 | VF2 | ok | tgccacctgacgtctaagaa | ||||||||||||||||
13 | BF8253 | 06-20 | O14 | pSB1C3-pKH7insert | 47.5 | 10.00 | 10.00 | 20 | 146695 | VF2 | ok | tgccacctgacgtctaagaa | ||||||||||||||||
14 | BF8254 | 06-20 | O15 | pSB1C3-pKH18insert | 50 | 8.00 | 12.00 | 20 | 146695 | VF2 | negativ, araC | tgccacctgacgtctaagaa | ||||||||||||||||
15 | 146695 | 06-20 | VF2 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | tgccacctgacgtctaagaa | #N/A | |||||||||||||||||
16 | 146696 | 06-20 | VR | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | attaccgcctttgagtgagc | #N/A | |||||||||||||||||
17 | BF8253 | 06-20 | O13 | pSB1C3-pKH6insert | 20 | 146696 | VR | ok | attaccgcctttgagtgagc | |||||||||||||||||||
18 | BF8254 | 06-20 | O14 | pSB1C3-pKH7insert | 20 | 146696 | VR | ok | attaccgcctttgagtgagc | |||||||||||||||||||
19 | BF8255 | 06-20 | O15 | pSB1C3-pKH18insert | 20 | 146696 | VR | negativ, Shewanella violacea | attaccgcctttgagtgagc | |||||||||||||||||||
20 | II2224 | 06-26 | O31 | pRARE | 50 | 13.42 | 6.58 | 20 | #N/A | ok, aber falsche Richtung | #N/A | |||||||||||||||||
21 | II2225 | 06-27 | O23 | pRSFDuet-1-mDNMT1(731-1602) | 95.65 | 10.00 | 10.00 | 20 | 146697 | #N/A | ok | #N/A | ||||||||||||||||
22 | II2227 | 06-27 | O23 | pRSFDuet-1-mDNMT1(731-1602) | 20 | 146698 | #N/A | ok | #N/A | |||||||||||||||||||
23 | II2228 | 06-27 | O23 | pRSFDuet-1-mDNMT1(731-1602) | 20 | 146699 | #N/A | ok | #N/A | |||||||||||||||||||
24 | II2229 | 06-27 | O23 | pRSFDuet-1-mDNMT1(731-1602) | 20 | 146700 | #N/A | ok | #N/A | |||||||||||||||||||
25 | II2232 | 06-27 | O17 | pHYRSF1 | 37.5 | 2.29 | 17.71 | 20 | T7 | ok | FALSE | |||||||||||||||||
26 | II2233 | 06-27 | O18 | pHYRSF1-02 | 37.5 | 2.85 | 17.15 | 20 | T7 | ok | FALSE | |||||||||||||||||
27 | II2234 | 06-27 | O19 | pJJDuet30 | 37.5 | 2.52 | 17.48 | 20 | T7 | Primer hat an 2 Stellen gebunden, sieht aber ok aus | FALSE | |||||||||||||||||
28 | II2235 | 06-27 | O20 | pSFBAD09 | 37.5 | 12.61 | 7.39 | 20 | pBAD-FP | ok | FALSE | |||||||||||||||||
29 | II2236 | 06-27 | O24 | pIT77 | 37.5 | 14.31 | 5.69 | 20 | pBR3 | ok | FALSE | |||||||||||||||||
30 | II2237 | 06-27 | O25 | pIT29 | 37.5 | 9.64 | 10.36 | 20 | pBR1 | ok | FALSE | |||||||||||||||||
31 | II2238 | 06-27 | O26 | pIT21 | 37.5 | 12.11 | 7.89 | 20 | pBR3 | ok | FALSE | |||||||||||||||||
32 | II2239 | 06-27 | O27 | pCL20 | 37.5 | 18.47 | 1.53 | 20 | pBR3 | ok | FALSE td> | |||||||||||||||||
33 | II2240 | 06-27 | O28 | pAU07 | 37.5 | 10.50 | 9.50 | 20 | pBAD-FP | ok | FALSE | |||||||||||||||||
34 | II2241 | 06-27 | O29 | pVS41 | 37.5 | 6.54 | 13.46 | 20 | pBR3 | ok | FALSE | |||||||||||||||||
35 | II2242 | 06-27 | O30 | pVS07 | 37.5 | 4.95 | 15.05 | 20 | pET-RP | ok | FALSE | |||||||||||||||||
36 | II2226 | 07-04 | O36 | pSB1AK8-BBa_I712074 | 50 | 17.24 | 2.76 | 20 | 937413 | VF2 | RFC23 (missing the final G in the prefix and the primary T in the suffix) | tgccacctgacgtctaagaa | ||||||||||||||||
37 | 892511 | II2230 | 07-04 | O35-2 | pSB1C3-2ZSspH6 | 50 | 9.48 | 10.52 | 20 | 937413 | VF2 | ok | tgccacctgacgtctaagaa | |||||||||||||||
38 | 892511 | II2243 | 07-04 | O35-2 | pSB1C3-2ZSspH6 | 20 | 937414 | VR | ok | attaccgcctttgagtgagc | ||||||||||||||||||
39 | 892511 | II2231 | 07-04 | O35-3 | pSB1C3-2ZSspH6 | 50 | 9.43 | 10.57 | 20 | 937413 | VF2 | ok | tgccacctgacgtctaagaa | |||||||||||||||
40 | 892511 | II2244 | 07-04 | O35-3 | pSB1C3-2ZSspH6 | 20 | 937414 | VR | ok | attaccgcctttgagtgagc | ||||||||||||||||||
41 | 937413 | VF2 | 10.00 | 0 | 20 | tgccacctgacgtctaagaa | FALSE | |||||||||||||||||||||
42 | 937414 | VR | 10.00 | 0 | 20 | attaccgcctttgagtgagc | FALSE | td> | ||||||||||||||||||||
43 | II2247 | 07-04 | O16 | pGBMCS-SortA | 40 | 13.47 | 6.53 | 20 | #N/A | ok | #N/A | |||||||||||||||||
44 | 893266 | II2245 | 07-08 | O32-2 | pSB1C3-2ZNpuH6 | 50 | 8.03 | 11.97 | 20 | 937413 | VF2 | insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part | tgccacctgacgtctaagaa | |||||||||||||||
45 | II2246 | 07-08 | O32-2 | pSB1C3-2ZNpuH6 | 0 | 0.00 | 0 | 20 | 937414 | VR | insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part | attaccgcctttgagtgagc | ||||||||||||||||
46 | II2248 | 07-08 | O33 | pSB1C3-2ZNpu | 50 | 10.40 | 9.60 | 20 | 937413 | VF2 | insertion in the prefix, additional His after Flag | tgccacctgacgtctaagaa | ||||||||||||||||
47 | II2249 | 07-08 | O33 | pSB1C3-2ZNpu | 0 | 0.00 | 0 | 20 | 937414 | VR | bad sequencing | attaccgcctttgagtgagc | ||||||||||||||||
48 | II2250 | 07-08 | O43 | pSB1C3-BBa_I746907 | 31.2 | 16.00 | 4.00 | 20 | 937413 | VF2 | tgccacctgacgtctaagaa | |||||||||||||||||
49 | II2251 | 07-08 | O43 | pSB1C3-BBa_I746907 | 0 | 0.00 | 0 | 20 | 937414 | VR | attaccgcctttgagtgagc | |||||||||||||||||
50 | 893901 | II2252 | 07-08 | O33-2 | pSB1C3-2ZNpu | 50 | 15.26 | 4.74 | 20 | 937413 | VF2 | additional His after flag, insertion in the prefix | tgccacctgacgtctaagaa | |||||||||||||||
51 | II2253 | 07-08 | O33-3 | pSB1C3-2ZNpu | 50 | 14.09 | 5.91 | 20 | 937413 | VF2 | additional His after flag, insertion in the prefix | tgccacctgacgtctaagaa | ||||||||||||||||
52 | 896202 | II2257 | O45-5 | pSB1C3-2ZNpuH6:GFP | 50 | 6.00 | 14.00 | 20 | 937415 | VF2 | no sequencing | tgccacctgacgtctaagaa | ||||||||||||||||
53 | 896202 | II2256 | O45-5 | pSB1C3-2ZNpuH6:GFP | 0 | 937416 | VR | we realized that this is npu (O45), has a mutation A1197del | attaccgcctttgagtgagc | |||||||||||||||||||
54 | 896202 | II2255 | O44-4 | pSB1C3-2ZSspH6:GFP | 50 | 4.56 | 15.44 | 20 | 937415 | VF2 | no sequencing | tgccacctgacgtctaagaa | ||||||||||||||||
55 | 896202 | II2254 | O44-4 | pSB1C3-2ZSspH6:GFP | 0 | 937416 | Vr | we realized that "ist glaube ich Ssp_DnaE. hat funktioniert", besides that ok | attaccgcctttgagtgagc | |||||||||||||||||||
56 | 937415 | VF2 td> | #N/A | 10.00 | 10.00 | 20 | #N/A | tgccacctgacgtctaagaa | #N/A | |||||||||||||||||||
57 | 937416 | VR | #N/A | 10.00 | 10.00 | 20 | #N/A | attaccgcctttgagtgagc | #N/A | |||||||||||||||||||
58 | II2258 | 07-21 | O59 | pSBX1K3-BBa_I13507 | 40 | 5.57 | 14.43 | 20 | 937415 | VF2 | bad sequencing | tgccacctgacgtctaagaa | ||||||||||||||||
59 | II2259 | 07-21 | O58 | pSBX1C3-BBa_I13507 | 40 | 8.00 | 12.00 | 20 | 937415 | VF2 | bad sequencing | tgccacctgacgtctaagaa | ||||||||||||||||
60 | II2260 | 07-21 | O57 | pSBX1A3-BBa_I13507 | 40 | 6.15 | 13.85 | 20 | 937415 | VF2 | bad sequencing | tgccacctgacgtctaagaa | ||||||||||||||||
61 | II2261 | 07-21 | O59-2 | pSBX1K3-BBa_I13507 | 40 | 10.47 | 9.53 | 20 | 937415 | VF2 | bad sequencing | tgccacctgacgtctaagaa | ||||||||||||||||
62 | II2262 | 07-21 | O58-2 | pSBX1C3-BBa_I13507 | 40 | 5.98 | 14.02 | 20 | 937415 | VF2 | pre insert insert containing ecoRI site | tgccacctgacgtctaagaa | ||||||||||||||||
63 | II2263 | 07-21 | O47 | pSB1AK8-T7-2ZNpu | 51.825 | 3.00 | 17.00 | 20 | 937415 | VF2 | tgccacctgacgtctaagaa | |||||||||||||||||
64 | II2264 | 07-21 | O48 | pSB1AK8-T7-2ZNpu:(GFP-H6) | 42.6 | 3.00 | 17.00 | 20 | #N/A | #N/A | ||||||||||||||||||
65 | II2265 | 07-21 | O47 | pSB1AK8-T7-2ZNpu | 51.825 | 3.00 | 17.00 | 20 | 937416 | VR | attaccgcctttgagtgagc | td> | ||||||||||||||||
66 | II2266 | 07-21 | O48 | pSB1AK8-T7-2ZNpu:(GFP-H6) | 42.6 | 3.00 | 17.00 | 20 | #N/A | #N/A | ||||||||||||||||||
67 | FALSE | |||||||||||||||||||||||||||
68 | 899248 | II2267 | 07-22 | O55 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 46.425 | 3.00 | 17.00 | 20 | #N/A | #N/A | |||||||||||||||||
69 | 899248 | II2268 | 07-22 | O55 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 0 | 0.00 | 0 | 20 | #N/A | #N/A | |||||||||||||||||
70 | II2269 | 07-24 | O55-2 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 42.775 | 10.00 | 10 | 20 | #N/A | #N/A | ||||||||||||||||||
71 | II2270 | 07-24 | O55-3 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 36.5 | 2.50 | 17.50 | 20 | #N/A | #N/A | ||||||||||||||||||
72 | II2271 | 07-24 | O55-4 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 39.57 | 4.00 | 16.00 | 20 | #N/A | #N/A | ||||||||||||||||||
73 | 20278 | 07-28 | VF2 | 10.00 | should be 20 | tgccacctgacgtctaagaa | FALSE | |||||||||||||||||||||
74 | 655423 | FG1165 | 07-28 | O57-2 | pSBX1A3-BBa_I13507 | 40 | 8.80 | 11.20 | 20 | 20278 | VF2 | sequenzing starts ok, then mixture? | O57-2: pSBX1A3-BBa_I13507 | tgccacctgacgtctaagaa | ||||||||||||||
75 | 655423 | FG1166 | 07-28 | O58-5 | pSBX1C3-BBa_I13507 | 40 | 20.00 | 0.00 | 20 | 20278 | VF2 | bad sequenzing | O58-5: pSBX1C3-BBa_I13507 | tgccacctgacgtctaagaa | ||||||||||||||
76 | 655423 | FG1167 | 07-28 | O59-3 | pSBX1K3-BBa_I13507 | 40 | 6.08 | 13.92 | 20 | 20278 | VF2 | frameshift, two fucking huge insertions | O59-3: pSBX1K3-BBa_I13507 | tgccacctgacgtctaagaa | ||||||||||||||
77 | 655423 | FG1168 | 07-28 | O60 | pSBX1T3-BBa_I13507 | 40 | 10.19 | 9.81 | 20 | 20278 | VF2 | ok | juhhuuu | O60: pSBX1T3-BBa_I13507 | tgccacctgacgtctaagaa | |||||||||||||
78 | 655423 | FG1169 | 07-28 | O61 | pSBX4A5-BBa_I13507 | 40 | 7.40 | 12.60 | 20 | 20278 | VF2 | starts ok then mixture | O61: pSBX4A5-BBa_I13507 | tgccacctgacgtctaagaa | ||||||||||||||
79 | 655423 | FG1170 | 07-28 | O62 | pSBX4C5-BBa_I13507 | 40 | 1.40 | 18.60 | 20 | 20278 | VF2 | ok | O62: pSBX4C5-BBa_I13507 | tgccacctgacgtctaagaa | ||||||||||||||
80 | 655423 | FG1171 | 07-28 | O63-2 | pSBX4K5-BBa_I13507 | 40 | 14.65 | 5.35 | 20 | 20278 | VF2 | no sequenzing | O63-2: pSBX4K5-BBa_I13507 | tgccacctgacgtctaagaa | td> | |||||||||||||
81 | 20279 | 08-04 | VF2.2 | FALSE | ||||||||||||||||||||||||
82 | 20280 | VF2.3 | FALSE | |||||||||||||||||||||||||
83 | 20281 | VF2.4 | FALSE | |||||||||||||||||||||||||
84 | 905285 | FG1173 | 08-04 | O57-3 | pSBX1A3-BBa_I13507 | 40 | 9.64 | 10.36 | 20 | 20279 | VF2.2 | bad sequencing | pSBX1A3-BBa_I13507 : O57-3 | FALSE | ||||||||||||||
85 | 905285 | FG1174 | 08-04 | O57-4 | pSBX1A3-BBa_I13507 | 40 | 10.54 | 9.46 | 20 | 20279 | VF2.2 | bad sequencing | pSBX1A3-BBa_I13507 : O57-4 | FALSE | ||||||||||||||
86 | 905285 | FG1175 | 08-04 | O58-4 | pSBX1C3-BBa_I13507 | 40 | 16.36 | 3.64 | 20 | 20279 | VF2.2 | ok | pSBX1C3-BBa_I13507 : O58-4 | FALSE | ||||||||||||||
87 | 905285 | FG1176 | 08-04 | O58-6 | pSBX1C3-BBa_I13507 | 40 | 19.09 | 0.91 | 20 | 20279 | VF2.2 | bad sequencing | pSBX1C3-BBa_I13507 : O58-6 | FALSE | ||||||||||||||
88 | 905285 | FG1177 | 08-04 | O59-3 | pSBX1K3-BBa_I13507 | 40 | 6.08 | 13.92 | 20 | 20279 | VF2.2 | ok,longest | pSBX1K3-BBa_I13507 : O59-3 | FALSE | ||||||||||||||
89 | 905285 | FG1178 | 08-04 | O59-4 | pSBX1K3-BBa_I13507 | 40 | 7.15 | 12.85 | 20 | 20279 | VF2.2 | ok | pSBX1K3-BBa_I13507 : O59-4 | FALSE | ||||||||||||||
90 | 905285 | FG1179 | 08-04 | O59-5 | pSBX1K3-BBa_I13507 | 40 | 6.00 | 14.00 | 20 | 20279 | VF2.2 | ok, short read | pSBX1K3-BBa_I13507 : O59-5 | FALSE | ||||||||||||||
91 | 905285 | FG1180 | 08-04 | O60-3 | pSBX1T3-BBa_I13507 | 40 | 10.01 | 9.99 | 20 | 20279 | VF2.2 | ok | pSBX1T3-BBa_I13507 : O60-3 | FALSE | ||||||||||||||
92 | 905285 | FG1181 | 08-04 | O61-2 | pSBX4A5-BBa_I13507 | 40 | 17.04 | 2.96 | 20 | 20280 | VF2.3 | bad sequencing | pSBX4A5-BBa_I13507 : O61-2 | FALSE | ||||||||||||||
93 | 905285 | FG1182 | 08-04 | O61-3 | pSBX4A5-BBa_I13507 | 40 | 11.79 | 8.21 | 20 | 20280 | VF2.3 | bad sequencing | pSBX4A5-BBa_I13507 : O61-3 | FALSE | ||||||||||||||
94 | 905285 | FG1183 | 08-04 | O61-4 | pSBX4A5-BBa_I13507 | 40 | 7.37 | 12.63 | 20 | 20280 | VF2.3 | ok | pSBX4A5-BBa_I13507 : O61-4 | FALSE | ||||||||||||||
95 | 905285 | FG1184 | 08-04 | O62-6 | pSBX4C5-BBa_I13507 | 40 | 15.18 | 4.82 | 20 | 20280 | VF2.3 | ok, longest | pSBX4C5-BBa_I13507 : O62-6 | FALSE | ||||||||||||||
96 | 905285 | FG1185 | 08-04 | O62-9 | pSBX4C5-BBa_I13507 | 40 | 39.80 | -19.80 | 20 | 20280 | VF2.3 | ok | pSBX4C5-BBa_I13507 : O62-9 | FALSE | ||||||||||||||
97 | 905285 | FG1186 | 08-04 | O62-10 | pSBX4C5-BBa_I13507 | 40 | 11.13 | 8.87 | 20 | 20280 | VF2.3 | ok | pSBX4C5-BBa_I13507 : O62-10 | FALSE | ||||||||||||||
98 | 905285 | FG1187 | 08-04 | O63 | pSBX4K5-BBa_I13507 | 40 | 7.93 | 12.07 | 20 | 20280 | VF2.3 | bad sequencing | pSBX4K5-BBa_I13507 : O63 | FALSE | ||||||||||||||
99 | 905285 | FG1188 | 08-04 | O63-3 | pSBX4K5-BBa_I13507 | 40 | 20.22 | -0.22 | 20 | 20280 | VF2.3 | bad sequencing | pSBX4K5-BBa_I13507 : O63-3 | FALSE | ||||||||||||||
100 | 905285 | FG1189 | 08-04 | O63-4 | pSBX4K5-BBa_I13507 | 40 | 11.13 | 8.87 | 20 | 20281 | VF2.4 | ok | pSBX4K5-BBa_I13507 : O63-4 | FALSE | ||||||||||||||
101 | 906555 | FG1190 | 08-07 | O71-2 | pSBX1T3-T7RBS-IGEMHD_v3 | 40 | 13.99 | 6.01 | 20 | 20281 | VF2.4 | good sequencing, ok | pSBX1T3-T7RBS-IGEMHD_v3 - O71-2 | FALSE | ||||||||||||||
102 | FG1191 | 08-07 | O72 | pSBX4A5-T7RBS-IGEMHD_v3 | 40 | 4.35 | 15.65 | 20 | 20281 | VF2.4 | good sequencing, ok | pSBX4A5-T7RBS-IGEMHD_v3 - O72 | FALSE | |||||||||||||||
103 | 906555 | FG1192 | 08-07 | O73 | pSBX4C5-T7RBS-IGEMHD_v3 | 40 | 1.83 | 18.17 | 20 | 20281 | VF2.4 | good sequencing, ok | pSBX4C5-T7RBS-IGEMHD_v3 - O73 | FALSE | ||||||||||||||
104 | 906555 | FG1193 | 08-07 | O65-7 | pSB1C3-BBa_J04450 | 40 | 9.30 | 10.70 | 20 | 20281 | VF2.4 | ok | pSB1C3-BBa_J04450 - O65-7 | FALSE | ||||||||||||||
105 | 906555 | FG1194 | 08-07 | O88 | pSB1C3-BBa_J52008 | 40 | 11.43 | 8.57 | 20 | 20281 | VF2.4 | ok | pSB1C3-BBa_J52008 - O88 | FALSE | ||||||||||||||
106 | 906555 | FG1195 | 08-07 | O89 | pSB1AK8-BBa_I712019 | 40 | 4.57 | 15.43 | 20 | 20281 | VF2.4 | ok, good | pSB1AK8-BBa_I712019 - O89 | FALSE | ||||||||||||||
107 | 906555 | FG1196 | 08-07 | O90 | pSB1C3-BBa_J06702 | 40 | 11.43 | 8.57 | 20 | 20281 | VF2.4 | ok, good | pSB1C3-BBa_J06702 - O90 | FALSE | ||||||||||||||
108 | 906555 | FG1197 | 08-07 | O91 | pSB1A2-BBa_E0040 | 40 | 6.67 | 13.33 | 20 | 20282 | VF2.5 | ok, good | pSB1A2-BBa_E0040 - O91 | FALSE | ||||||||||||||
109 | 20282 | 08-07 | VF2.5 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | tgccacctgacgtctaagaa | #N/A | #N/A | ||||||||||||||||
110 | 906555 | FG1198 | 08-07 | O57-2 | pSBX1A3-BBa_I13507 | GATC-VF2-980198 | VF2 | ok | tgccacctgacgtctaagaa | |||||||||||||||||||
111 | 906555 | FG1199 | 08-07 | O59-3 | pSBX1K3-BBa_I13507 td> | GATC-VR-980199 | VR | attaccgcctttgagtgagc | ||||||||||||||||||||
112 | 20283 | 08-11 | VR2.5 | #N/A | #N/A | 20.00 | 0.00 | 20 | #N/A | #N/A | #N/A | |||||||||||||||||
113 | 20284 | 08-11 | VF2.6 | #N/A | #N/A | 20.00 | 0.00 | 20 | #N/A | #N/A | #N/A | |||||||||||||||||
114 | 907965 | FG1203 | 08-11 | O80-3 | pSBX4C5-2ZSspH6:GFP | 40 | #DIV/0! | #DIV/0! | 20 | 20283 | VR2.5 | ok | pSBX4C5-2ZSspH6:GFP - O80-3 | FALSE | ||||||||||||||
115 | 907965 | FG1204 | 08-11 | O81-2 | pSBX4C5-2ZNpuH6:GFP | 40 | 2.57 | 17.43 | 20 | 20283 | VR2.5 | pSBX4C5-2ZSspH6:GFP | pSBX4C5-2ZNpuH6:GFP - O81-2 | FALSE | ||||||||||||||
116 | 907965 | FG1205 | 08-11 | O86 | pSB1AK8-BBa_I712074-2ZSspH6:GFP | 40 | 7.14 | 12.86 | 20 | 20283 | VR2.5 | is just pSB1AK8-BBa_I712074 | pSB1AK8-BBa_I712074-2ZSspH6:GFP - O86 | FALSE | ||||||||||||||
117 | 907965 | FG1206 | 08-11 | O87 | pSB1AK8-BBa_I712074-2ZNpuH6:GFP | 40 | 4.44 | 15.56 | 20 | 20283 | VR2.5 | mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown | pSB1AK8-BBa_I712074-2ZNpuH6:GFP - O87 | FALSE | ||||||||||||||
118 | 907965 | FG1207 | 08-11 | O48-2 | pSB1AK8-T7-2ZNpu:(GFP-H6) | 40 | 8.36 | 11.64 | 20 | 20283 | VR2.5 | ok | pSB1AK8-T7-2ZNpu:(GFP-H6) - O48-2 | FALSE | ||||||||||||||
119 | 907965 | FG1208 | 08-11 | O80-3 | pSBX4C5-2ZSspH6:GFP | 40 | #DIV/0! | #DIV/0! | 20 | 20284 | VF2.6 | ok | pSBX4C5-2ZSspH6:GFP - O80-3 | FALSE | ||||||||||||||
120 | 907965 | FG1209 | 08-11 | O81-2 | pSBX4C5-2ZNpuH6:GFP | 40 | 2.57 | 17.43 | 20 | 20284 | VF2.6 | pSBX4C5-2ZSspH6:GFP | pSBX4C5-2ZNpuH6:GFP - O81-2 | FALSE | ||||||||||||||
121 | 907965 | FG1210 | 08-11 | O86 | pSB1AK8-BBa_I712074-2ZSspH6:GFP | 40 | 7.14 | 12.86 | 20 | 20284 | VF2.6 | is just pSB1AK8-BBa_I712074 | pSB1AK8-BBa_I712074-2ZSspH6:GFP - O86 | FALSE | td> | |||||||||||||
122 | 907965 | FG1211 | 08-11 | O87 | pSB1AK8-BBa_I712074-2ZNpuH6:GFP | 40 | 4.44 | 15.56 | 20 | 20284 | VF2.6 | mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown | pSB1AK8-BBa_I712074-2ZNpuH6:GFP - O87 | FALSE | ||||||||||||||
123 | 907965 | FG1212 | 08-11 | O48-2 | pSB1AK8-T7-2ZNpu:(GFP-H6) | 40 | 8.36 | 11.64 | 20 | 20284 | VF2.6 | ok | pSB1AK8-T7-2ZNpu:(GFP-H6) - O48-2 | FALSE | ||||||||||||||
124 | GATC-VF2-980198 | VF2 | FALSE | |||||||||||||||||||||||||
125 | GATC-VR-980199 | VR | FALSE | |||||||||||||||||||||||||
126 | 908645 | FG1200 | 08-13 | O102-3 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 40 | 10.47 | 9.53 | 20 | GATC-VF2-980198 | VF2 | ok | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 - O102-3 | tgccacctgacgtctaagaa | ||||||||||||||
127 | 908645 | FG1201 | 08-13 | O102-4 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 40 | 11.38 | 8.62 | 20 | GATC-VF2-980198 | VF2 | ok | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 - O102-4 | tgccacctgacgtctaagaa | ||||||||||||||
128 | 908645 | FG1202 | 08-13 | O102-7 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 40 | 9.77 | 10.23 | 20 | GATC-VF2-980198 | VF2 | ok | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 - O102-7 | tgccacctgacgtctaagaa | ||||||||||||||
129 | 909228 | FG1213 | 08-14 | O103 | pSBX1K3-2Zv2Npu | 40 | 12.18 | 7.82 | 20 | GATC-VF2-980198 | VF2 | wrong, charlie's stuff | pSBX1K3-2Zv2Npu - O103 | tgccacctgacgtctaagaa | ||||||||||||||
130 | 909228 | FG1214 | 08-14 | O103 | pSBX1K3-2Zv2Npu | 40 | 12.18 | 7.82 | 20 | GATC-VR-980199 | VR | wrong, charlie's stuff | pSBX1K3-2Zv2Npu - O103 | attaccgcctttgagtgagc | ||||||||||||||
131 | 909228 | II2277 | 08-14 | O104 | pSB1C3-BBa_I746908_sfGFP | 40 | 8.15 | 11.85 | 20 | GATC-VR-980199 | VR | ok | pSB1C3-BBa_I746908_sfGFP - O104 | 1228 | 63 | attaccgcctttgagtgagc | ||||||||||||
132 | 909228 | II2278 | 08-14 | O104 | pSB1C3-BBa_I746908_sfGFP | 40 | 8.15 | 11.85 | 20 | GATC-VF2-980198 | VF2 | ok | pSB1C3-BBa_I746908_sfGFP - O104 | 1632 | 85 | tgccacctgacgtctaagaa | ||||||||||||
133 | 909624 | II2279 | 08-15 | O103-2 | pSBX1K3-2Zv2Npu | 40 | 8.61 | 11.39 | 20 | GATC-VF2-980198 | VF2 | ok (silent mutation in selection marker) | pSBX1K3-2Zv2Npu - O103-2 | tgccacctgacgtctaagaa | ||||||||||||||
134 | 909624 | II2280 | 08-15 | O103-2 | pSBX1K3-2Zv2Npu | 40 | 8.61 | 11.39 | 20 | GATC-VR-980199 | VR | ok (silent mutation in selection marker) | pSBX1K3-2Zv2Npu - O103-2 | attaccgcctttgagtgagc | ||||||||||||||
135 | 908645 | II2274 | 08-13 | O102-3 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 40 | 10.47 | 9.53 | 20 td> | GATC-VR-980199 | VR | ok | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 - O102-3 | attaccgcctttgagtgagc | ||||||||||||||
136 | 908645 | II2275 | 08-13 | O102-4 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 40 | 11.38 | 8.62 | 20 | GATC-VR-980199 | VR | ok | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 - O102-4 | attaccgcctttgagtgagc | ||||||||||||||
137 | 908645 | II2276 | 08-13 | O102-7 | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 | 40 | 9.77 | 10.23 | 20 | GATC-VR-980199 | VR | ok | pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3 - O102-7 | attaccgcctttgagtgagc | ||||||||||||||
138 | Max W. ist voll der Spasti | II2273 | 08-18 | O98-4 | pSBX4C5-2Zv2Npu | 43.9 | 2.00 | 18.00 | 20 | GATC-VF2-980198 | VF2 | ok | pSBX4C5-2Zv2Npu - O98-4 | 1906 | 86.9 | tgccacctgacgtctaagaa | ||||||||||||
139 | II2281 | 08-18 | O55-5 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 42.275 | 2.50 | 17.50 | 20 | GATC-VF2-980199 | #N/A | pSB1A3-Smt3-2ZSortA-H6 (ccdB) - O55-5 td> | #N/A | ||||||||||||||||
140 | II2282 | 08-18 | O55-6 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 38.525 | 5.00 | 15.00 | 20 | GATC-VF2-980200 | #N/A | pSB1A3-Smt3-2ZSortA-H6 (ccdB) - O55-6 | #N/A | ||||||||||||||||
141 | II2283 | 08-18 | O55-7 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 32.975 | 5.00 | 15.00 | 20 | GATC-VF2-980201 | #N/A | pSB1A3-Smt3-2ZSortA-H6 (ccdB) - O55-7 | #N/A | ||||||||||||||||
142 | II2284 | 08-18 | O55-8 | pSB1A3-Smt3-2ZSortA-H6 (ccdB) | 40.575 | 5.00 | 15.00 | 20 | GATC-VF2-980202 | #N/A | pSB1A3-Smt3-2ZSortA-H6 (ccdB) - O55-8 | #N/A | ||||||||||||||||
143 | II2285 | 08-18 | O116 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 55.7 | 5.00 | 15.00 | 20 | GATC-VF2-980203 | #N/A | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) - O116 | #N/A | ||||||||||||||||
144 | II2286 | 08-18 | O116-2 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 47.375 | 5.00 | 15.00 | 20 | GATC-VF2-980204 | #N/A | single base deletion | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) - O116-2 | #N/A td> | |||||||||||||||
145 | II2287 | 08-18 | O116-3 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 60.75 | 5.00 | 15.00 | 20 | GATC-VF2-980205 | #N/A | two-base deletion and point mutation | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) - O116-3 | #N/A | |||||||||||||||
146 | 910172 | II2288 | 08-18 | O109 | pSB1C3-BBa_K808000 | 37.5 | 2.00 | 18.00 | 20 | GATC-VF2-980206 | #N/A | ok | pSB1C3-BBa_K808000 - O109 | #N/A | ||||||||||||||
147 | 910172 | II2289 | 08-18 | O117-2 | pSB1C3-BBa_K741002 | 38.85 | 3.00 | 17.00 | 20 | GATC-VF2-980207 | #N/A | ok | pSB1C3-BBa_K741002 - O117-2 | #N/A | ||||||||||||||
148 | 912532 | II2290 | 08-21 | O123 | pSBX1K3-1GateNpuDnaE(C) | 40 | 3.09 | 16.91 | 20 | GATC-VF2-980198 | VF2 | mutation A220G K74E in the mRFP CDS, besides ok | pSBX1K3-1GateNpuDnaE(C) - O123 | tgccacctgacgtctaagaa | ||||||||||||||
149 | 912532 | II2291 | 08-22 | O123 | pSBX1K3-1GateNpuDnaE(C) | 40 | 2.50 | 17.50 | 20 | GATC-VR-980199 | VR | mutation A220G K74E in the mRFP CDS, besides ok | pSBX1K3-1GateNpuDnaE(C) - O123 | attaccgcctttgagtgagc | ||||||||||||||
150 | 913391 | II2294 | 08-25 | O111-2 | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) | 40 | 9.86 | 10.14 | 20 | GATC-VF2-980198 | VF2 | EINE, 1, EINE fucking mutation......... | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) - O111-2 | tgccacctgacgtctaagaa | ||||||||||||||
151 | 913391 | II2295 | 08-25 | O111-2 | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) | 40 | 9.86 | 10.14 | 20 | GATC-VR-980199 | VR | EINE, 1, EINE fucking mutation......... | pSBX1K3-2Zv2Npu:(LambdaLys-Rigid) - O111-2 | attaccgcctttgagtgagc | ||||||||||||||
152 | 913391 | II2296 | 08-25 | O112-2 | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His | 40 | 2.29 | 17.71 | 20 | GATC-VF2-980198 | VF2 | ok | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His - O112-2 | tgccacctgacgtctaagaa | ||||||||||||||
153 | 913391 | II2297 | 08-25 | O112-2 | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His | 40 | 2.29 | 17.71 | 20 | GATC-VR-980199 | VR | ok | pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His - O112-2 td> | attaccgcctttgagtgagc | ||||||||||||||
154 | 913391 | II2298 | 08-25 | O113-2 | pSBX1K3-2Zv2Npu:Xyla_circ_noHis | 40 | 2.68 | 17.32 | 20 | GATC-VF2-980198 | VF2 | fürn arsch | pSBX1K3-2Zv2Npu:Xyla_circ_noHis - O113-2 | tgccacctgacgtctaagaa | ||||||||||||||
155 | 913391 | II2299 | 08-25 | O113-2 | pSBX1K3-2Zv2Npu:Xyla_circ_noHis | 40 | 2.68 | 17.32 | 20 | GATC-VR-980199 | VR | fürn arsch | pSBX1K3-2Zv2Npu:Xyla_circ_noHis - O113-2 | attaccgcctttgagtgagc | ||||||||||||||
156 | 913391 | II2300 | 08-25 | O124-2 | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext | 40 | 2.80 | 17.20 | 20 | GATC-VF2-980198 | VF2 | fürn arsch | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext - O124-2 | tgccacctgacgtctaagaa | ||||||||||||||
157 | 913391 | II2301 | 08-25 | O124-2 | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext | 40 | 2.80 | 17.20 | 20 | GATC-VR-980199 | VR | fürn arsch | pSBX1K3-2Zv2Npu:Xyla(H6)destrCext - O124-2 | attaccgcctttgagtgagc | td> | |||||||||||||
158 | 912972 | II2302 | 08-25 | O94-3 | pSBX4C5-2GateNpuDnaE(N) | 40 | 2.61 | 17.39 | 20 | GATC-VF2-980198 | VF2 | bad sequencing, but probaBIy ok | pSBX4C5-2GateNpuDnaE(N) - O94-3 | tgccacctgacgtctaagaa | ||||||||||||||
159 | 912972 | II2303 | 08-25 | O94-4 | pSBX4C5-2GateNpuDnaE(N) | 40 | 3.20 | 16.80 | 20 | GATC-VF2-980198 | VF2 | bad sequencing, but probaBIy ok | pSBX4C5-2GateNpuDnaE(N) - O94-4 | tgccacctgacgtctaagaa | ||||||||||||||
160 | 912972 | II2304 | 08-25 | O95-3 | pSBX4C5-2GateNpuDnaE(C) | 40 | 2.88 | 17.12 | 20 | GATC-VF2-980198 | VF2 | bad sequencing, but probaBIy ok | pSBX4C5-2GateNpuDnaE(C) - O95-3 | tgccacctgacgtctaagaa | ||||||||||||||
161 | 912972 | II2305 | 08-25 | O95-4 | pSBX4C5-2GateNpuDnaE(C) | 40 | 2.61 | 17.39 | 20 | GATC-VF2-980198 | VF2 | bad sequencing, but probaBIy ok | pSBX4C5-2GateNpuDnaE(C) - O95-4 | tgccacctgacgtctaagaa | ||||||||||||||
162 | 912972 | II2306 | 08-25 | O94-3 | pSBX4C5-2GateNpuDnaE(N) | 40 | 2.61 | 17.39 | 20 | GATC-VR-980199 | VR | bad sequencing, but probaBIy ok | pSBX4C5-2GateNpuDnaE(N) - O94-3 | attaccgcctttgagtgagc | ||||||||||||||
163 | 912972 | II2307 | 08-25 | O94-4 | pSBX4C5-2GateNpuDnaE(N) | 40 | 3.20 | 16.80 | 20 | GATC-VR-980199 | VR | bad sequencing, but probaBIy ok | pSBX4C5-2GateNpuDnaE(N) - O94-4 | attaccgcctttgagtgagc | ||||||||||||||
164 | 912972 | II2308 | 08-25 | O95-3 | pSBX4C5-2GateNpuDnaE(C) | 40 | 2.88 | 17.12 | 20 | GATC-VR-980199 | VR | bad sequencing, but probaBIy ok | pSBX4C5-2GateNpuDnaE(C) - O95-3 | attaccgcctttgagtgagc | ||||||||||||||
165 | 912972 | II2309 | 08-25 | O95-4 | pSBX4C5-2GateNpuDnaE(C) | 40 | 2.61 | 17.39 | 20 | GATC-VR-980199 | VR | bad sequencing, but probaBIy ok | pSBX4C5-2GateNpuDnaE(C) - O95-4 | attaccgcctttgagtgagc | ||||||||||||||
166 | 912972 | II2310 | 08-25 | O86-3 | pSB1AK8-BBa_I712074-2ZSspH6:GFP | 40 | 2.22 | 17.78 td> | 20 | GATC-VF2-980198 | VF2 | ok | pSB1AK8-BBa_I712074-2ZSspH6:GFP - O86-3 | tgccacctgacgtctaagaa | ||||||||||||||
167 | 912972 | II2311 | 08-25 | O86-3 | pSB1AK8-BBa_I712074-2ZSspH6:GFP | 40 | 2.22 | 17.78 | 20 | GATC-VR-980199 | VR | ok | pSB1AK8-BBa_I712074-2ZSspH6:GFP - O86-3 | attaccgcctttgagtgagc | ||||||||||||||
168 | 912997 | II2312 | 08-25 | O105-2 | pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 | 40 | 3.60 | 16.40 | 20 | GATC-VF2-980198 | VF2 | ok | pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 - O105-2 | tgccacctgacgtctaagaa | ||||||||||||||
169 | 912997 | II2313 | 08-25 | O105-2 | pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 | 40 | 3.60 | 16.40 | 20 | GATC-VR-980199 | VR | ok, C->T in VF2 site | pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6 - O105-2 | attaccgcctttgagtgagc | ||||||||||||||
170 | 912997 | II2314 | 08-25 | O106-2 | pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 | 40 | 2.79 | 17.21 | 20 | GATC-VF2-980198 | VF2 | mixture A64->T mutation | pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 - O106-2 | tgccacctgacgtctaagaa | ||||||||||||||
171 | 912997 | II2315 | 08-25 | O106-2 | pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 | 40 | 2.79 | 17.21 | 20 | GATC-VR-980199 | VR | mixture A64->Tmutation | pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6 - O106-2 | attaccgcctttgagtgagc | ||||||||||||||
172 | 912997 | II2316 | 08-25 | O107 | pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 | 40 | 2.96 | 17.04 | 20 | GATC-VF2-980198 | VF2 | ok, good | pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 - O107 | tgccacctgacgtctaagaa | ||||||||||||||
173 | 912997 | II2317 | 08-25 | O107 | pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 | 40 | 2.96 | 17.04 | 20 | GATC-VR-980199 | VR | ok, C->T in VF2 site | pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6 - O107 | attaccgcctttgagtgagc | ||||||||||||||
174 | 912997 | II2318 | 08-25 | O108 | pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 | 40 | 3.38 | 16.62 | 20 | GATC-VF2-980198 | VF2 | 1bp deletion before final H6, 1bp deletion in suffix | pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 - O108 | tgccacctgacgtctaagaa | ||||||||||||||
175 | 912997 | II2319 | 08-25 | O108 | pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 | 40 | 3.38 | 16.62 | 20 | GATC-VR-980199 | VR | 1bp deletion before final H6, 1bp deletion in suffix | pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6 - O108 | attaccgcctttgagtgagc | ||||||||||||||
176 | 913352 | II2320 | 08-26 | O122-3 | pSBX1K3-1GateNpuDnaE(N) | 40 | 2.80 | 17.20 | 20 | VF2 | ok, perfect | pSBX1K3-1GateNpuDnaE(N) - O122-3 | tgccacctgacgtctaagaa | |||||||||||||||
177 | 913352 | II2321 | 08-26 | O122-3 | pSBX1K3-1GateNpuDnaE(N) | 40 | 2.80 | 17.20 | 20 | VR | ok, perfect | pSBX1K3-1GateNpuDnaE(N) - O122-3 | attaccgcctttgagtgagc | |||||||||||||||
178 | 913352 | II2322 | 08-26 | O123-5 | pSBX1K3-1GateNpuDnaE(C) | 40 | 3.85 | 16.15 | 20 | VF2 | ok, perfect | pSBX1K3-1GateNpuDnaE(C) - O123-5 | tgccacctgacgtctaagaa | |||||||||||||||
179 | 913352 | II2323 | 08-26 | O123-5 | pSBX1K3-1GateNpuDnaE(C) | 40 | 3.85 | 16.15 | 20 | VR | ok, perfect | pSBX1K3-1GateNpuDnaE(C) - O123-5 | attaccgcctttgagtgagc | |||||||||||||||
180 | BI2115 | 09-04 | O160 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 3.00 | 17.00 | 20 | VF2 | mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R) | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160 | tgccacctgacgtctaagaa | ||||||||||||||||
181 | BI2116 | 09-04 | O160 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 3.00 | 17.00 | 20 | VR | mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R) | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160 | attaccgcctttgagtgagc | ||||||||||||||||
182 | BI2117 | 09-04 | O160-2 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 3.00 | 17.00 | 20 | VF2 | bad sequencing | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160-2 | tgccacctgacgtctaagaa | ||||||||||||||||
183 | BI2118 | 09-04 | O160-2 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 3.00 | 17.00 | 20 | VR | auch eine in mrfp | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160-2 | attaccgcctttgagtgagc | ||||||||||||||||
184 | BI2119 | 09-04 | O130 | pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG | 40 | 13.42 | 6.58 | 20 | VF2 | pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG - O130 | tgccacctgacgtctaagaa | |||||||||||||||||
185 | BI2120 | 09-04 | O130 | pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG | 40 | 13.42 | 6.58 | 20 | VR | pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG - O130 | attaccgcctttgagtgagc | |||||||||||||||||
186 | BI2121 | 09-04 | O145 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 - O145 | tgccacctgacgtctaagaa | |||||||||||||||||
187 | BI2122 | 09-04 | O145 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-mRFPn1-154-NpuN-H6 - O145 | attaccgcctttgagtgagc | |||||||||||||||||
188 | BI2123 | 09-04 | O146 | pSBX4C5-H6-NpuC-mRFP155-225-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-NpuC-mRFP155-225-H6 - O146 | tgccacctgacgtctaagaa | |||||||||||||||||
189 | BI2124 | 09-04 | O146 | pSBX4C5-H6-NpuC-mRFP155-225-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-NpuC-mRFP155-225-H6 - O146 | attaccgcctttgagtgagc | |||||||||||||||||
190 | BI2125 | 09-04 | O147 | pSBX4C5-H6-mRFP1-168-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-mRFP1-168-NpuN-H6 - O147 | tgccacctgacgtctaagaa | |||||||||||||||||
191 | BI2126 | 09-04 | O147 | pSBX4C5-H6-mRFP1-168-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-mRFP1-168-NpuN-H6 - O147 | attaccgcctttgagtgagc | |||||||||||||||||
192 | BI2127 | 09-04 | O148 | pSBX4C5-H6-NpuC-mRFP169-225-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-NpuC-mRFP169-225-H6 - O148 | tgccacctgacgtctaagaa | |||||||||||||||||
193 | BI2128 | 09-04 | O148 | pSBX4C5-H6-NpuC-mRFP169-225-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-NpuC-mRFP169-225-H6 - O148 | attaccgcctttgagtgagc | |||||||||||||||||
194 | BI2129 | 09-04 | O149 | pSBX4C5-H6-RenLucN-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-RenLucN-NpuN-H6 - O149 | tgccacctgacgtctaagaa | |||||||||||||||||
195 | BI2130 | 09-04 | O149 | pSBX4C5-H6-RenLucN-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-RenLucN-NpuN-H6 - O149 | attaccgcctttgagtgagc | |||||||||||||||||
196 | BI2131 | 09-04 | O150 | pSBX4C5-H6-NpuC-RenLucC-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-NpuC-RenLucC-H6 - O150 | tgccacctgacgtctaagaa | |||||||||||||||||
197 | BI2132 | 09-04 | O150 | pSBX4C5-H6-NpuC-RenLucC-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-NpuC-RenLucC-H6 - O150 | attaccgcctttgagtgagc | |||||||||||||||||
198 | BI2133 | 09-04 | O151 | pSBX4C5-H6-FireLucN-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-FireLucN-NpuN-H6 - O151 | tgccacctgacgtctaagaa | |||||||||||||||||
199 | BI2134 | 09-04 | O151 | pSBX4C5-H6-FireLucN-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-FireLucN-NpuN-H6 - O151 | attaccgcctttgagtgagc | |||||||||||||||||
200 | BI2135 | 09-04 | O152 | pSBX4C5-H6-NpuC-FireLucC-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-NpuC-FireLucC-H6 - O152 | tgccacctgacgtctaagaa | td> | ||||||||||||||||
201 | BI2136 | 09-04 | O152 | pSBX4C5-H6-NpuC-FireLucC-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-NpuC-FireLucC-H6 - O152 | attaccgcctttgagtgagc | |||||||||||||||||
202 | BI2137 | 09-04 | O153 | pSBX4C5-H6-mCherryN-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-mCherryN-NpuN-H6 - O153 | tgccacctgacgtctaagaa | |||||||||||||||||
203 | BI2138 | 09-04 | O153 | pSBX4C5-H6-mCherryN-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-mCherryN-NpuN-H6 - O153 | attaccgcctttgagtgagc | |||||||||||||||||
204 | BI2139 | 09-04 | O154 | pSBX4C5-H6-NpuC-mCherryC-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-NpuC-mCherryC-H6 - O154 | tgccacctgacgtctaagaa | |||||||||||||||||
205 | BI2140 | 09-04 | O154 | pSBX4C5-H6-NpuC-mCherryC-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-NpuC-mCherryC-H6 - O154 | attaccgcctttgagtgagc | |||||||||||||||||
206 | BI2141 | 09-04 | O155 | pSBX4C5-H6-GFPn1-64-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-GFPn1-64-NpuN-H6 - O155 | tgccacctgacgtctaagaa | |||||||||||||||||
207 | BI2142 | 09-04 | O155 | pSBX4C5-H6-GFPn1-64-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-GFPn1-64-NpuN-H6 - O155 | attaccgcctttgagtgagc | |||||||||||||||||
208 | BI2143 | 09-04 | O157 | pSBX4C5-H6-GFPn1-157-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VF2 | pSBX4C5-H6-GFPn1-157-NpuN-H6 - O157 | tgccacctgacgtctaagaa | |||||||||||||||||
209 | BI2144 | 09-04 | O157 | pSBX4C5-H6-GFPn1-157-NpuN-H6 | 40 | 1.33 | 18.67 | 20 | VR | pSBX4C5-H6-GFPn1-157-NpuN-H6 - O157 | attaccgcctttgagtgagc | |||||||||||||||||
210 | BI2147 | 9/5/2014 | O160-3 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 2.15 | 17.85 | 20 | VF2 | insertion in smt3 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160-3 | tgccacctgacgtctaagaa | ||||||||||||||||
211 | BI2148 | 09-05 | O160-3 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 2.15 | 17.85 | 20 | VR | silent in mfrp | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160-3 | attaccgcctttgagtgagc | ||||||||||||||||
212 | BI2149 | 09-05 | O160-4 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 2.07 | 17.93 | 20 | VF2 | ok | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160-4 | tgccacctgacgtctaagaa | ||||||||||||||||
213 | BI2150 | 09-05 | O160-4 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 2.07 | 17.93 | 20 | VR | ok (silent in mrfp) | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160-4 | attaccgcctttgagtgagc | ||||||||||||||||
214 | BI2151 | 09-08 | O145-3 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 | 40 | 3.02 | 16.98 | 20 | VR | ok | pSBX4C5-H6-mRFPn1-154-NpuN-H6 - O145-3 | attaccgcctttgagtgagc | ||||||||||||||||
215 | BI2152 | 09-08 | O146-3 | pSBX4C5-H6-NpuC-mRFP155-225-H6 | 40 | 2.42 | 17.58 | 20 | VR | ok | pSBX4C5-H6-NpuC-mRFP155-225-H6 - O146-3 | attaccgcctttgagtgagc | ||||||||||||||||
216 | BI2153 | 09-08 | O147-3 | pSBX4C5-H6-mRFP1-168-NpuN-H6 | 40 | 2.17 | 17.83 | 20 | VR | kok | pSBX4C5-H6-mRFP1-168-NpuN-H6 - O147-3 | attaccgcctttgagtgagc | ||||||||||||||||
217 | BI2154 | 09-08 | O148-3 | pSBX4C5-H6-NpuC-mRFP169-225-H6 | 40 | 3.20 | 16.80 | 20 | VR | ok | pSBX4C5-H6-NpuC-mRFP169-225-H6 - O148-3 | attaccgcctttgagtgagc | ||||||||||||||||
218 | BI2155 | 09-08 | O149-3 | pSBX4C5-H6-RenLucN-NpuN-H6 | 40 | 4.68 | 15.32 | 20 | VR | ok, one read bad, silent mutation in ligation renlucN to NpuN | pSBX4C5-H6-RenLucN-NpuN-H6 - O149-3 | attaccgcctttgagtgagc | ||||||||||||||||
219 | BI2156 | 09-08 | O150-3 | pSBX4C5-H6-NpuC-RenLucC-H6 | 40 | 4.26 | 15.74 | 20 | VR | ok, TAA insertion after His Tag | td> | pSBX4C5-H6-NpuC-RenLucC-H6 - O150-3 | attaccgcctttgagtgagc | |||||||||||||||
220 | BI2157 | 09-08 | O151-3 | pSBX4C5-H6-FireLucN-NpuN-H6 | 40 | 4.94 | 15.06 | 20 | VR | ok | pSBX4C5-H6-FireLucN-NpuN-H6 - O151-3 | attaccgcctttgagtgagc | ||||||||||||||||
221 | BI2158 | 09-08 | O152-3 | pSBX4C5-H6-NpuC-FireLucC-H6 | 40 | 2.45 | 17.55 | 20 | VR | ok | pSBX4C5-H6-NpuC-FireLucC-H6 - O152-3 | attaccgcctttgagtgagc | ||||||||||||||||
222 | BI2159 | 09-08 | O153-3 | pSBX4C5-H6-mCherryN-NpuN-H6 | 40 | 4.73 | 15.27 | 20 | VR | ok | pSBX4C5-H6-mCherryN-NpuN-H6 - O153-3 | attaccgcctttgagtgagc | ||||||||||||||||
223 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | FALSE | |||||||||||||||||||||
224 | BI2160 | 09-08 | O155-3 | pSBX4C5-H6-GFPn1-64-NpuN-H6 | 40 | 4.17 | 15.83 | 20 | VR | ok | pSBX4C5-H6-GFPn1-64-NpuN-H6 - O155-3 | attaccgcctttgagtgagc | ||||||||||||||||
225 | BI2161 | 09-08 | O157-3 | pSBX4C5-H6-GFPn1-157-NpuN-H6 | 40 | 1.24 | 18.76 | 20 | VR | ok | pSBX4C5-H6-GFPn1-157-NpuN-H6 - O157-3 | attaccgcctttgagtgagc | ||||||||||||||||
226 | BI2162 | 09-08 | O158-3 | pSBX4C5-H6-GFPc158-238-NpuC-H6 | 40 | 1.25 | 18.75 | 20 | VR | ok, one shift after "stem loop" | pSBX4C5-H6-GFPc158-238-NpuC-H6 - O158-3 | attaccgcctttgagtgagc | ||||||||||||||||
227 | BI2163 | 09-08 | O145-3 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 | 40 | 3.02 | 16.98 | 20 | VF2 | pSBX4C5-H6-mRFPn1-154-NpuN-H6 - O145-3 | tgccacctgacgtctaagaa | |||||||||||||||||
228 | BI2164 | 09-08 | O146-3 | pSBX4C5-H6-NpuC-mRFP155-225-H6 | 40 | 2.42 | 17.58 | 20 | VF2 | pSBX4C5-H6-NpuC-mRFP155-225-H6 - O146-3 | tgccacctgacgtctaagaa | |||||||||||||||||
229 | BI2165 | 09-08 | O147-3 | pSBX4C5-H6-mRFP1-168-NpuN-H6 | 40 | 2.17 | 17.83 | 20 | VF2 | pSBX4C5-H6-mRFP1-168-NpuN-H6 - O147-3 | tgccacctgacgtctaagaa | |||||||||||||||||
230 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | FALSE | |||||||||||||||||||||
231 | BI2166 | 09-08 | O149-3 | pSBX4C5-H6-RenLucN-NpuN-H6 | 40 | 4.68 | 15.32 | 20 | VF2 | pSBX4C5-H6-RenLucN-NpuN-H6 - O149-3 | tgccacctgacgtctaagaa | |||||||||||||||||
232 | #N/A | #N/A | #N/A | 20 | - | FALSE | ||||||||||||||||||||||
233 | BI2167 | 09-08 | O151-3 | pSBX4C5-H6-FireLucN-NpuN-H6 | 40 | 4.94 | 15.06 | 20 | VF2 | pSBX4C5-H6-FireLucN-NpuN-H6 - O151-3 | tgccacctgacgtctaagaa | |||||||||||||||||
234 | #N/A | #N/A | #N/A | #N/A | 20 | VF2 | #N/A | tgccacctgacgtctaagaa | ||||||||||||||||||||
235 | BI2168 | 09-08 | O153-3 | pSBX4C5-H6-mCherryN-NpuN-H6 | 40 | 4.73 | 15.27 | 20 | VF2 | pSBX4C5-H6-mCherryN-NpuN-H6 - O153-3 | tgccacctgacgtctaagaa | |||||||||||||||||
236 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | FALSE | |||||||||||||||||||||
237 | BI2169 | 09-08 | O155-3 | pSBX4C5-H6-GFPn1-64-NpuN-H6 | 40 | 4.17 | 15.83 | 20 | VF2 | pSBX4C5-H6-GFPn1-64-NpuN-H6 - O155-3 | tgccacctgacgtctaagaa | |||||||||||||||||
238 | BI2170 | 09-08 | O157-3 | pSBX4C5-H6-GFPn1-157-NpuN-H6 | 40 | 1.24 | 18.76 | 20 | VF2 | pSBX4C5-H6-GFPn1-157-NpuN-H6 - O157-3 | tgccacctgacgtctaagaa | |||||||||||||||||
239 | BI2145 | 09-08 | O163 | pSBX4C5-2GateSspDnaX-S11C | 40 | 4. 26 | 15.74 | 20 | VF2 | ok | pSBX4C5-2GateSspDnaX-S11C - O163 | tgccacctgacgtctaagaa | ||||||||||||||||
240 | BI2171 | 09-08 | O163 | pSBX4C5-2GateSspDnaX-S11C | 40 | 4.26 | 15.74 | 20 | VR | ok | pSBX4C5-2GateSspDnaX-S11C - O163 | attaccgcctttgagtgagc | ||||||||||||||||
241 | BI2172 | 09-08 | O164 | pSBX4C5-2GateSspDnaX-S11C HN->AG | 40 | 2.96 | 17.04 | 20 | VF2 | ok | pSBX4C5-2GateSspDnaX-S11C HN->AG - O164 | tgccacctgacgtctaagaa | ||||||||||||||||
242 | BI2173 | 09-08 | O164 | pSBX4C5-2GateSspDnaX-S11C HN->AG | 40 | 2.96 | 17.04 | 20 | VR | ok | pSBX4C5-2GateSspDnaX-S11C HN->AG - O164 | attaccgcctttgagtgagc | ||||||||||||||||
243 | BI2174 | 09-08 | O165 | pSBX4C5-2GateSspDnaX-S11N | 40 | 4.00 | 16.00 | 20 | VF2 | part of intein+mrfp not sequenced | pSBX4C5-2GateSspDnaX-S11N - O165 | tgccacctgacgtctaagaa | ||||||||||||||||
244 | BI2175 | 09-08 | O165 | pSBX4C5-2GateSspDnaX-S11N | 40 | 4.00 | 16.00 | 20 | VR | part of intein+mrfp not sequenced | pSBX4C5-2GateSspDnaX-S11N - O165 | attaccgcctttgagtgagc | ||||||||||||||||
245 | BI2176 | 09-08 | O166 | pSBX4C5-2GateSspDnaX-S11N C->G | 40 | 15.10 | 4.90 | 20 | VF2 | part of intein+mrfp not sequenced | pSBX4C5-2GateSspDnaX-S11N C->G - O166 | tgccacctgacgtctaagaa | ||||||||||||||||
246 | BI2177 | 09-08 | O166 | pSBX4C5-2GateSspDnaX-S11N C->G | 40 | 15.10 | 4.90 | 20 | VR | part of intein+mrfp not sequenced | pSBX4C5-2GateSspDnaX-S11N C->G - O166 | attaccgcctttgagtgagc | ||||||||||||||||
247 | BI2178 | 09-08 | O167 | pSBX4C5-2GateSspGyrB-S11C | 40 | 3.42 | 16.58 | 20 | VF2 | ok | pSBX4C5-2GateSspGyrB-S11C - O167 | tgccacctgacgtctaagaa | ||||||||||||||||
248 | BI2179 | 09-08 | O167 | pSBX4C5-2GateSspGyrB-S11C | 40 | 3.42 | 16.58 | 20 | VR | ok | pSBX4C5-2GateSspGyrB-S11C - O167 | attaccgcctttgagtgagc | ||||||||||||||||
249 | BI2180 | 09-08 | O168 | pSBX4C5-2GateSspGyrB-S11C SN->AG | 40 | 3.41 | 16.59 | 20 | VF2 | ok | pSBX4C5-2GateSspGyrB-S11C SN->AG - O168 | tgccacctgacgtctaagaa | ||||||||||||||||
250 | BI2181 | 09-08 | O168 | pSBX4C5-2GateSspGyrB-S11C SN->AG | 40 | 3.41 | 16.59 | 20 | VR | ok | pSBX4C5-2GateSspGyrB-S11C SN->AG - O168 | attaccgcctttgagtgagc | ||||||||||||||||
251 | BI2182 | 09-08 | O169 | pSBX4C5-2GateSspGyrB-S11N | 40 | 3.90 | 16.10 | 20 | VF2 | part of intein+mrfp not sequenced | pSBX4C5-2GateSspGyrB-S11N - O169 | tgccacctgacgtctaagaa | ||||||||||||||||
252 | BI2183 | 09-08 | O169 | pSBX4C5-2GateSspGyrB-S11N | 40 | 3.90 | 16.10 | 20 | VR | part of intein+mrfp not sequenced, mutation in ccdb c->a (?) | pSBX4C5-2GateSspGyrB-S11N - O169 | attaccgcctttgagtgagc | ||||||||||||||||
253 | BI2184 | 09-08 | O171 | pSBX4C5-2GateTerDnaE3-S11C | 40 | 3.32 | 16.68 | 20 | VF2 | ok | pSBX4C5-2GateTerDnaE3-S11C - O171 | tgccacctgacgtctaagaa | ||||||||||||||||
254 | BI2185 | 09-08 | O171 | pSBX4C5-2GateTerDnaE3-S11C | 40 | 3.32 | 16.68 | 20 | VR | ok | pSBX4C5-2GateTerDnaE3-S11C - O171 | attaccgcctttgagtgagc | ||||||||||||||||
255 | BI2186 | 09-08 | O172 | pSBX4C5-2GateTerDnaE3-S11C SN->AG | 40 | 4.51 | 15.49 | 20 | VF2 | ok | pSBX4C5-2GateTerDnaE3-S11C SN->AG - O172 | tgccacctgacgtctaagaa | ||||||||||||||||
256 | BI2187 | 09-08 | O172 | pSBX4C5-2GateTerDnaE3-S11C SN->AG | 40 | 4.51 | 15.49 | 20 | VR | ok | pSBX4C5-2GateTerDnaE3-S11C SN->AG - O172 | attaccgcctttgagtgagc | ||||||||||||||||
257 | BI2188 | 09-08 | O173 | pSBX4C5-2GateTerDnaE3-S11N | 40 | 11.02 | 8.98 | 20 | VF2 | mutation in lacO-reg g->t, part of mrfp+intein not sequenced | pSBX4C5-2GateTerDnaE3-S11N - O173 | tgccacctgacgtctaagaa | td> | |||||||||||||||
258 | BI2189 | 09-08 | O173 | pSBX4C5-2GateTerDnaE3-S11N | 40 | 11.02 | 8.98 | 20 | VR | part of mrfp+intein not sequenced | pSBX4C5-2GateTerDnaE3-S11N - O173 | attaccgcctttgagtgagc | ||||||||||||||||
259 | BI2190 | 09-08 | O174 | pSBX4C5-2GateTerDnaE3-S11N C->G | 40 | 3.51 | 16.49 | 20 | VF2 | no mutations | pSBX4C5-2GateTerDnaE3-S11N C->G - O174 | tgccacctgacgtctaagaa | ||||||||||||||||
260 | BI2191 | 09-08 | O174 | pSBX4C5-2GateTerDnaE3-S11N C->G | 40 | 3.51 | 16.49 | 20 | VR | begins in ccdb --> not fully seq, mut. in ccdb ga->tc | pSBX4C5-2GateTerDnaE3-S11N C->G - O174 | attaccgcctttgagtgagc | ||||||||||||||||
261 | BI2192 | 09-08 | O143 | pSBX4C5-2GateNpuDnaE(N)* | 40 | 4.20 | 15.80 | 20 | VF2 | part of intein/mrfp not sequenced | pSBX4C5-2GateNpuDnaE(N)* - O143 | tgccacctgacgtctaagaa | ||||||||||||||||
262 | BI2193 | 09-08 | O143 | pSBX4C5-2GateNpuDnaE(N)* | 40 | 4.20 | 15.80 | 20 | VR | part of intein/mrfp not sequenced | pSBX4C5-2GateNpuDnaE(N)* - O143 | attaccgcctttgagtgagc | ||||||||||||||||
263 | BI2196 | 09-08 | O162-2 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 40 | 22.66 | -2.66 | 20 | VF2 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) - O162-2 | tgccacctgacgtctaagaa | |||||||||||||||||
264 | BI2197 | 09-08 | O162-2 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) | 40 | 22.66 | -2.66 | 20 | VR | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB) - O162-2 | attaccgcctttgagtgagc | |||||||||||||||||
265 | AF9230 | 09-08 | O160-4 | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu | 40 | 2.07 | 17.93 | 20 | 20285 | VF2-C7T | pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu - O160-4 | FALSE | ||||||||||||||||
266 | 20285 | 09-08 | VF2-C7T | 233 | #N/A | 20.00 | 0 | 20 | VF2-C7T | 233 - VF2-C7T | FALSE | |||||||||||||||||
267 | BI2198 | 09-10 | O169-2 | pSBX4C5-2GateSspGyrB-S11N | 40 td> | 2.36 | 17.64 | 20 | VF2 | ok | ?, Jan | pSBX4C5-2GateSspGyrB-S11N - O169-2 | tgccacctgacgtctaagaa | |||||||||||||||
268 | BI2199 | 09-10 | O169-2 | pSBX4C5-2GateSspGyrB-S11N | 40 | 2.36 | 17.64 | 20 | VR | ok (mut in ccdb E87*) | ?, Jan | pSBX4C5-2GateSspGyrB-S11N - O169-2 | attaccgcctttgagtgagc | |||||||||||||||
269 | BI2200 | 09-10 | O173-2 | pSBX4C5-2GateTerDnaE3-S11N | 40 | 8.38 | 11.62 | 20 | VF2 | ok | ?, Jan | pSBX4C5-2GateTerDnaE3-S11N - O173-2 | tgccacctgacgtctaagaa | |||||||||||||||
270 | BI2201 | 09-10 | O173-2 | pSBX4C5-2GateTerDnaE3-S11N | 40 | 8.38 | 11.62 | 20 | VR | ok | ?, Jan | pSBX4C5-2GateTerDnaE3-S11N - O173-2 | attaccgcctttgagtgagc | |||||||||||||||
271 | BI2202 | 09-10 | O174-2 | pSBX4C5-2GateTerDnaE3-S11N C->G | 40 | 6.23 | 13.77 | 20 | VF2 | ok | ?, Jan | pSBX4C5-2GateTerDnaE3-S11N C->G - O174-2 | tgccacctgacgtctaagaa | td> | ||||||||||||||
272 | BI2203 | 09-10 | O174-2 | pSBX4C5-2GateTerDnaE3-S11N C->G | 40 | 6.23 | 13.77 | 20 | VR | ok | ?, Jan | pSBX4C5-2GateTerDnaE3-S11N C->G - O174-2 | attaccgcctttgagtgagc | |||||||||||||||
273 | BI2204 | 09-10 | O143-2 | pSBX4C5-2GateNpuDnaE(N)* | 40 | 5.68 | 14.32 | 20 | VF2 | ok | ?, Jan | pSBX4C5-2GateNpuDnaE(N)* - O143-2 | tgccacctgacgtctaagaa | |||||||||||||||
274 | BI2205 | 09-10 | O143-2 | pSBX4C5-2GateNpuDnaE(N)* | 40 | 5.68 | 14.32 | 20 | VR | ok | ?, Jan | pSBX4C5-2GateNpuDnaE(N)* - O143-2 | attaccgcctttgagtgagc | |||||||||||||||
275 | BI2206 | 09-10 | O144-2 | pSBX4C5-2GateNpuDnaE(C)* | 40 | 4.42 | 15.58 | 20 | VF2 | ok | ?, Jan | pSBX4C5-2GateNpuDnaE(C)* - O144-2 | tgccacctgacgtctaagaa | |||||||||||||||
276 | BI2207 | 09-10 | O144-2 | pSBX4C5-2GateNpuDnaE(C)* | 40 | 4. 42 | 15.58 | 20 | VR | ok | ?, Jan | pSBX4C5-2GateNpuDnaE(C)* - O144-2 | attaccgcctttgagtgagc | |||||||||||||||
277 | BI2208 | 09-10 | O170 | pSBX4C5-2GateSspGyrB-S11N C->G | 40 | 4.19 | 15.81 | 20 | VF2 | ok | ?, Jan | pSBX4C5-2GateSspGyrB-S11N C->G - O170 | tgccacctgacgtctaagaa | |||||||||||||||
278 | BI2209 | 09-10 | O170 | pSBX4C5-2GateSspGyrB-S11N C->G | 40 | 4.19 | 15.81 | 20 | VR | ok | ?, Jan | pSBX4C5-2GateSspGyrB-S11N C->G - O170 | attaccgcctttgagtgagc | |||||||||||||||
279 | BI2210 | 09-10 | O135 | pSBX4C5-2GateGp41-1(N) | 40 | 6.67 | 13.33 | 20 | VF2 | ok | ?, Jan | pSBX4C5-2GateGp41-1(N) - O135 | tgccacctgacgtctaagaa | |||||||||||||||
280 | BI2211 | 09-10 | O135 | pSBX4C5-2GateGp41-1(N) | 40 | 6.67 | 13.33 | 20 | VR | ok | ?, Jan | pSBX4C5-2GateGp41-1(N) - O135 | attaccgcctttgagtgagc | td> | ||||||||||||||
281 | BI2212 | 09-10 | O136 | pSBX4C5-2GateGp41-1(C) | 40 | 4.82 | 15.18 | 20 | VF2 | ok | ?, Jan | pSBX4C5-2GateGp41-1(C) - O136 | tgccacctgacgtctaagaa | |||||||||||||||
282 | BI2213 | 09-10 | O136 | pSBX4C5-2GateGp41-1(C) | 40 | 4.82 | 15.18 | 20 | VR | ok | ?, Jan | pSBX4C5-2GateGp41-1(C) - O136 | attaccgcctttgagtgagc | |||||||||||||||
283 | BI1915 | 09-10 | O180 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 3.39 | 16.61 | 20 | VF2 | deletion in 1st Stop codon | Jan | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180 | tgccacctgacgtctaagaa | |||||||||||||||
284 | BI1916 | 09-10 | O180 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 3.39 | 16.61 | 20 | VR | deletion in 1st Stop codon | Jan | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180 | attaccgcctttgagtgagc | |||||||||||||||
285 | 920502 | 20286 | 09-11 | 213,Universal-
S11N_R BsmBI_ccdB | Universal-S11N_R BsmBI_ccdB | #N/A | #N/A | #N/A | 20 | #N/A | Universal-S11N_R BsmBI_ccdB - 213,Universal-S11N_R BsmBI_ccdB | #N/A | ||||||||||||||||
286 | 920502 | BI1917 | 09-11 | O165 | pSBX4C5-2GateSspDnaX-S11N | 40 | 4.00 | 16.00 | 20 | 20286 | 213,Universal-S11N_R BsmBI_ccdB | ok | ?, Jan | pSBX4C5-2GateSspDnaX-S11N - O165 | FALSE | |||||||||||||
287 | 920502 | BI1918 | 09-11 | O166 | pSBX4C5-2GateSspDnaX-S11N C->G | 40 | 15.10 | 4.90 | 20 | 20286 | 213,Universal-S11N_R BsmBI_ccdB | ok | ?, Jan | pSBX4C5-2GateSspDnaX-S11N C->G - O166 | FALSE | |||||||||||||
288 | 920502 | BI1919 | 09-11 | O169 | pSBX4C5-2GateSspGyrB-S11N | 40 | 3.90 | 16.10 | 20 | 20286 | 213,Universal-S11N_R BsmBI_ccdB | ok, only intein sequenzed | ?, Jan | pSBX4C5-2GateSspGyrB-S11N - O169 | FALSE | |||||||||||||
289 | 920502 | BI1920 | 09-11 | O170 | pSBX4C5-2GateSspGyrB-S11N C->G | 40 | 4.19 | 15.81 | 20 | 20286 | 213,Universal-S11N_R BsmBI_ccdB | ok | ?, Jan | pSBX4C5-2GateSspGyrB-S11N C->G - O170 | FALSE | |||||||||||||
290 | 920502 | BI1921 | 09-11 | O173-2 | pSBX4C5-2GateTerDnaE3-S11N | 40 | 8.38 | 11.62 | 20 | 20286 | 213,Universal-S11N_R BsmBI_ccdB | ok | ?, Jan | pSBX4C5-2GateTerDnaE3-S11N - O173-2 | FALSE | |||||||||||||
291 | 920502 | BI1922 | 09-11 | O174-2 | pSBX4C5-2GateTerDnaE3-S11N C->G | 40 | 6.23 | 13.77 | 20 | 20286 | 213,Universal-S11N_R BsmBI_ccdB | ok | ?, Jan | pSBX4C5-2GateTerDnaE3-S11N C->G - O174-2 | FALSE | |||||||||||||
292 | 920502 | BI1923 | 09-11 | O143-2 | pSBX4C5-2GateNpuDnaE(N)* | 40 | 5.68 | 14.32 | 20 | 20286 | 213,Universal-S11N_R BsmBI_ccdB | ok | ?, Jan | pSBX4C5-2GateNpuDnaE(N)* - O143-2 | FALSE | |||||||||||||
293 | 920502 | BI1924 | 09-11 | O135 | pSBX4C5-2GateGp41-1(N) | 40 | 6.67 | 13.33 | 20 | 20286 | 213,Universal-S11N_R BsmBI_ccdB | ok | ?, Jan | pSBX4C5-2GateGp41-1(N) - O135 | FALSE | td> | ||||||||||||
294 | BI12214 | 09-11 | O181-3 | pSBX1K3-Xylanase | 40 | 19.80 | 0.20 | 20 | VR | ok | pSBX1K3-Xylanase - O181-3 | attaccgcctttgagtgagc | ||||||||||||||||
295 | BI1925 | 09-11 | O125-10 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 41.25 | 10.00 | 10.00 | 20 | #N/A | pSB1C3-Smt3-2ZSortA-H6 (ccdB) - O125-10 | #N/A | |||||||||||||||||
296 | BI1926 | 09-11 | O177 | pSB1C3-2ZSortA-H6 | 41.37525 | 3.33 | 16.67 | 20 | VF2 | pSB1C3-2ZSortA-H6 - O177 | tgccacctgacgtctaagaa | |||||||||||||||||
297 | BI1927 | 09-11 | O177-2 | pSB1C3-2ZSortA-H6 | 42.54075 | 3.33 | 16.67 | 20 | VF2 | pSB1C3-2ZSortA-H6 - O177-2 | tgccacctgacgtctaagaa | |||||||||||||||||
298 | BI1928 | 09-11 | O177-3 | pSB1C3-2ZSortA-H6 | 37.21275 | 3.33 | 16.67 | 20 | VF2 | pSB1C3-2ZSortA-H6 - O177-3 | tgccacctgacgtctaagaa | |||||||||||||||||
299 | BI1929 | 09-11 | O178 | pSB1C3-H6-0ZSortA | 43.1235 | 3.33 | 16.67 | 20 | VF2 | ok | pSB1C3-H6-0ZSortA - O178 | tgccacctgacgtctaagaa | ||||||||||||||||
300 | BI1930 | 09-11 | O178-2 | pSB1C3-H6-0ZSortA | 45.954 | 3.33 | 16.67 | 20 | VF2 | ok | pSB1C3-H6-0ZSortA - O178-2 | tgccacctgacgtctaagaa | ||||||||||||||||
301 | BI1931 | 09-11 | O178-3 | pSB1C3-H6-0ZSortA | 47.86875 | 3.33 | 16.67 | 20 | VF2 | H9R mutation | pSB1C3-H6-0ZSortA - O178-3 | tgccacctgacgtctaagaa | ||||||||||||||||
302 | BI1932 | 09-11 | O125-10 | pSB1C3-Smt3-2ZSortA-H6 (ccdB) | 20 | VR | pSB1C3-Smt3-2ZSortA-H6 (ccdB) - O125-10 | attaccgcctttgagtgagc | ||||||||||||||||||||
303 | BI1933 | 09-11 | O177 | pSB1C3-2ZSortA-H6 | 20 | VR | pSB1C3-2ZSortA-H6 - O177 | attaccgcctttgagtgagc | ||||||||||||||||||||
304 | BI1934 | 09-11 | O177-2 | pSB1C3-2ZSortA-H6 | 20 | VR | pSB1C3-2ZSortA-H6 - O177-2 | attaccgcctttgagtgagc | ||||||||||||||||||||
305 | BI1935 | 09-11 | O177-3 | pSB1C3-2ZSortA-H6 | 20 | VR | pSB1C3-2ZSortA-H6 - O177-3 | attaccgcctttgagtgagc | ||||||||||||||||||||
306 | BI1936 | 09-11 | O178 | pSB1C3-H6-0ZSortA | 20 | VR | no result | pSB1C3-H6-0ZSortA - O178 | attaccgcctttgagtgagc | |||||||||||||||||||
307 | BI1937 | 09-11 | O178-2 | pSB1C3-H6-0ZSortA | 20 | VR | no result | pSB1C3-H6-0ZSortA - O178-2 | attaccgcctttgagtgagc | |||||||||||||||||||
308 | BI1938 | 09-11 | O178-3 | pSB1C3-H6-0ZSortA | 20 | VR | no result | pSB1C3-H6-0ZSortA - O178-3 | attaccgcctttgagtgagc | |||||||||||||||||||
309 | BI1939 | 09-12 | O188 | pSBX1K3-LiLaLy(H6) | 40 | 1.67 | 18.33 | 20 | VF2 | pSBX1K3-LiLaLy(H6) - O188 | tgccacctgacgtctaagaa | |||||||||||||||||
310 | BI1940 | 09-12 | O188 | pSBX1K3-LiLaLy(H6) | 40 | 1.67 | 18.33 | 20 | VR | luciferase?? | pSBX1K3-LiLaLy(H6) - O188 | attaccgcctttgagtgagc | ||||||||||||||||
311 | BI1941 | 09-12 | O188-3 | pSBX1K3-LiLaLy(H6) | 40 | 1.73 | 18.27 | 20 | VF2 | pSBX1K3-LiLaLy(H6) - O188-3 | tgccacctgacgtctaagaa | |||||||||||||||||
312 | BI1942 | 09-12 | O188-3 | pSBX1K3-LiLaLy(H6) | 40 | 1.73 | 18.27 | 20 | VR | luciferase?? | pSBX1K3-LiLaLy(H6) - O188-3 | attaccgcctttgagtgagc | ||||||||||||||||
313 | BI1943 | 09-12 | O189 | pSBX1K3-LiLaLy(H6) | 40 | 3.34 | 16.66 | 20 | VF2 | pSBX1K3-LiLaLy(H6) - O189 | tgccacctgacgtctaagaa | |||||||||||||||||
314 | BI1944 | 09-12 | O189 | pSBX1K3-LiLaLy(H6) | 40 | 3.34 | 16.66 | 20 | VR | is pSBX1K3-LiLaLy(H6) | pSBX1K3-LiLaLy(H6) - O189 | attaccgcctttgagtgagc | ||||||||||||||||
315 | BI1945 | 09-12 | O189-2 | pSBX1K3-LiLaLy(H6) | 40 | 3.89 | 16.11 | 20 | VF2 | pSBX1K3-LiLaLy(H6) - O189-2 | tgccacctgacgtctaagaa | |||||||||||||||||
316 | BI1946 | 09-12 | O189-2 | pSBX1K3-LiLaLy(H6) | 40 | 3.89 | 16.11 | 20 | VR | is pSBX1K3-LiLaLy(H6) | pSBX1K3-LiLaLy(H6) - O189-2 | attaccgcctttgagtgagc | ||||||||||||||||
317 | BI1947 | 09-12 | O181 | pSBX1K3-Xylanase | 40 | 20.94 | -0.94 | 20 | VR | ok | pSBX1K3-Xylanase - O181 | attaccgcctttgagtgagc | td> | |||||||||||||||
318 | 921065 | BI1948 | 09-12 | O182 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 40 | 1.17 | 18.83 | 20 | GATC-VR-980199 | VR | ok | Jan | pSBX4C5-mRFPn1-154_mRFPc155-255_coex - O182 | 1503 | 84.1 | attaccgcctttgagtgagc | |||||||||||
319 | 921065 | BI1949 | 09-12 | O182 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 40 | 1.17 | 18.83 | 20 | GATC-VF2-980198 | VF2 | ok | Jan | pSBX4C5-mRFPn1-154_mRFPc155-255_coex - O182 | tgccacctgacgtctaagaa | |||||||||||||
320 | 921065 | BI1950 | 09-12 | O182-2 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 40 | 1.37 | 18.63 | 20 | GATC-VR-980199 | VR | ok | Jan | pSBX4C5-mRFPn1-154_mRFPc155-255_coex - O182-2 | 1584 | 88.5 | attaccgcctttgagtgagc | |||||||||||
321 | 921065 | BI1951 | 09-12 | O182-2 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 40 | 1.37 | 18.63 | 20 | GATC-VF2-980198 | VF2 | ok | Jan | pSBX4C5-mRFPn1-154_mRFPc155-255_coex - O182-2 | tgccacctgacgtctaagaa | |||||||||||||
322 | BI1952 | 09-12 | O161 | pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI | 40 | 13.79 | 6.21 | 20 | GATC-VR-980199 | VR | hä? | pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI - O161 | attaccgcctttgagtgagc | |||||||||||||||
323 | BI1953 | 09-15 | O207-2 | pSB1C3-EX-BsaI-A-NN-IasB-S | 40 | 4.34 | 15.66 | 20 | GATC-VF2-980198 | VF2 | is O208-2......oo, 3.G afterprefix to AC | pSB1C3-EX-BsaI-A-NN-IasB-S - O207-2 | 1487 | 84 | tgccacctgacgtctaagaa | |||||||||||||
324 | BI1954 | 09-15 | O207-2 | pSB1C3-EX-BsaI-A-NN-IasB-S | 40 | 4.34 | 15.66 | 20 | GATC-VR-980199 | VR | is O208-2......oo, 3.G afterprefix to AC | pSB1C3-EX-BsaI-A-NN-IasB-S - O207-2 | 1563 | 84.2 | attaccgcctttgagtgagc | |||||||||||||
325 | BI1955 | 09-15 | O208-2 | pSB1C3-EX-BsaI-CC-O-IasB-S | 40 | 5.73 | 14.27 | 20 | GATC-VF2-980198 | VF2 | is O207-2....:(, 3.G afterprefix to AC | pSB1C3-EX-BsaI-CC-O-IasB-S - O208-2 | tgccacctgacgtctaagaa | |||||||||||||||
326 | BI1956 | 09-15 | O208-2 | pSB1C3-EX-BsaI-CC-O-IasB-S | 40 | 5.73 | 14.27 | 20 | GATC-VR-980199 | VR | is O207-2....:(, 3.G afterprefix to AC | pSB1C3-EX-BsaI-CC-O-IasB-S - O208-2 | 1615 | 88.3 | attaccgcctttgagtgagc | |||||||||||||
327 | 921651 | BI1959 | 09-15 | O206 | pSB4C5-GFP_contr | 40 | 1.56 | 18.44 | 20 | GATC-VF2-980198 | VF2 | ok | pSB4C5-GFP_contr - O206 | tgccacctgacgtctaagaa | ||||||||||||||
328 | 921651 | BI1960 | 09-15 | O206 | pSB4C5-GFP_contr | 40 | 1.56 | 18.44 | 20 | GATC-VR-980199 | VR | ok | pSB4C5-GFP_contr - O206 | attaccgcctttgagtgagc | ||||||||||||||
329 | 921651 | BI1961 | 09-15 | O205 | pSB4C5-mCherry_contr | 40 | 1.48 | 18.52 | 20 | GATC-VF2-980198 | VF2 | ok | pSB4C5-mCherry_contr - O205 | tgccacctgacgtctaagaa | ||||||||||||||
330 | 921651 | BI1962 | 09-15 | O205 | pSB4C5-mCherry_contr | 40 | 1.48 | 18.52 | 20 | GATC-VR-980199 | VR | ok | pSB4C5-mCherry_contr - O205 | attaccgcctttgagtgagc | ||||||||||||||
331 | 921651 | BI1957 | 09-15 | O179 | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6 | 40 | 1.77 | 18.23 | 20 | GATC-VF2-980198 | VF2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6 - O179 | tgccacctgacgtctaagaa | |||||||||||||||
332 | 921651 | AF9231 | 09-15 | O179 | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6 | 40 | 1.77 | 18.23 | 20 | GATC-VR-980199 | VR | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6 - O179 | attaccgcctttgagtgagc | |||||||||||||||
333 | 921651 | BI1958 | 09-15 | O179-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6 | 40 | 2.82 | 17.18 | 20 | GATC-VF2-980198 | VF2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6 - O179-2 | tgccacctgacgtctaagaa | |||||||||||||||
334 | 921651 | AF9232 | 09-15 | O179-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6 | 40 | 2.82 | 17.18 | 20 | GATC-VR-980199 | VR | pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6 - O179-2 | attaccgcctttgagtgagc | |||||||||||||||
335 | 921651 | BI1964 | 09-15 | O183 | pSBX4C5-mRFPn1-168-mRFPc169-255_coex | 40 | 2.19 | 17.81 | 20 | GATC-VF2-980198 | VF2 | ok | Jan | pSBX4C5-mRFPn1-168-mRFPc169-255_coex - O183 | tgccacctgacgtctaagaa | |||||||||||||
336 | 921651 | BI1965 | 09-15 | O183 | pSBX4C5-mRFPn1-168-mRFPc169-255_coex | 40 | 2.19 | 17.81 | 20 | GATC-VR-980199 | VR | ok | Jan | pSBX4C5-mRFPn1-168-mRFPc169-255_coex - O183 | attaccgcctttgagtgagc | td> | ||||||||||||
337 | 921651 | BI1966 | 09-15 | O186 | pSBX4C5mCherryN_mCherryC_coex | 40 | 1.72 | 18.28 | 20 | GATC-VF2-980198 | VF2 | ok | Jan | pSBX4C5mCherryN_mCherryC_coex - O186 | tgccacctgacgtctaagaa | |||||||||||||
338 | 921651 | BI1967 | 09-15 | O186 | pSBX4C5mCherryN_mCherryC_coex | 40 | 1.72 | 18.28 | 20 | GATC-VR-980199 | VR | ok | Jan | pSBX4C5mCherryN_mCherryC_coex - O186 | attaccgcctttgagtgagc | |||||||||||||
339 | 921651 | BI1968 | 09-15 | O187 | pSBX4C5-GFPn1-157_GFPc158-255_coex | 40 | 1.75 | 18.25 | 20 | GATC-VF2-980198 | VF2 | ok | Jan | pSBX4C5-GFPn1-157_GFPc158-255_coex - O187 | tgccacctgacgtctaagaa | |||||||||||||
340 | 921651 | BI1969 | 09-15 | O187 | pSBX4C5-GFPn1-157_GFPc158-255_coex | 40 | 1.75 | 18.25 | 20 | GATC-VR-980199 | VR | ok | Jan | pSBX4C5-GFPn1-157_GFPc158-255_coex - O187 | attaccgcctttgagtgagc | |||||||||||||
341 | 921651 | BI1970 | 09-15 | O180-2 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 4.00 | 16.00 | 20 | GATC-VF2-980198 | VF2 | deletion in 1st Stop codon | Jan | estimated concentration | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-2 | tgccacctgacgtctaagaa | ||||||||||||
342 | 921651 | BI1971 | 09-15 | O180-2 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 4.00 | 16.00 | 20 | GATC-VR-980199 | VR | deletion in 1st Stop codon | Jan | estimated concentration | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-2 | attaccgcctttgagtgagc | ||||||||||||
343 | 921651 | BI1972 | 09-15 | O182-3 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 40 | 1.37 | 18.63 | 20 | GATC-VF2-980198 | VF2 | ok | Jan | pSBX4C5-mRFPn1-154_mRFPc155-255_coex - O182-3 | tgccacctgacgtctaagaa | |||||||||||||
344 | 921651 | BI1973 | 09-15 | O182-3 | pSBX4C5-mRFPn1-154_mRFPc155-255_coex | 40 | 1.37 | 18.63 | 20 | GATC-VR-980199 | VR | ok | Jan | pSBX4C5-mRFPn1-154_mRFPc155-255_coex - O182-3 | 1460 | 84.5 | attaccgcctttgagtgagc | |||||||||||
345 | BI1963 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | #N/A | |||||||||||||||||||
346 | BI1974 | O189-4 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 40 | 3.90 | 16.10 | 20 | VF2 | ok | pSBX4C5-2Zv2Npu:LaLys+flexlinker - O189-4 | tgccacctgacgtctaagaa | |||||||||||||||||
347 | BI1975 | O189-4 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 40 | 3.90 | 16.10 | 20 | VR | bad seq | pSBX4C5-2Zv2Npu:LaLys+flexlinker - O189-4 | attaccgcctttgagtgagc | |||||||||||||||||
348 | BI1976 | O189-5 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 40 | 3.58 | 16.42 | 20 | VF2 | ok | pSBX4C5-2Zv2Npu:LaLys+flexlinker - O189-5 | tgccacctgacgtctaagaa | |||||||||||||||||
349 | BI1977 | O189-5 | pSBX4C5-2Zv2Npu:LaLys+flexlinker | 40 | 3.58 | 16.42 | 20 | VR | ok | pSBX4C5-2Zv2Npu:LaLys+flexlinker - O189-5 | attaccgcctttgagtgagc | |||||||||||||||||
350 | BI1978 | O216-2 | pSBX1K3-XylaH6BB | 40 | 5.54 | 14.46 | 20 | VR | ok | pSBX1K3-XylaH6BB - O216-2 | attaccgcctttgagtgagc | |||||||||||||||||
351 | BI1979 | O217 | pSBX1K3-XylaH6extBB | 40 | 4.67 | 15.33 | 20 | VR | bad seq | pSBX1K3-XylaH6extBB - O217 | attaccgcctttgagtgagc | |||||||||||||||||
352 | 922764 | BI1980 | 09-17 | O192 | pSBX4C5-1GateNpuDnaE(N)*-H6 | 40 | 1.97 | 18.03 | 20 | VR | looks like pSBX4C5-1GateNpuDnaEC_SN->AG_H6 | pSBX4C5-1GateNpuDnaE(N)*-H6 - O192 | attaccgcctttgagtgagc | |||||||||||||||
353 | 922764 | BI1982 | 09-17 | O193 | pSBX4C5-1GateSspDnaX-S11(C)-H6 | 40 | 2.26 | 17.74 | 20 | VF | ok | pSBX4C5-1GateSspDnaX-S11(C)-H6 - O193 | FALSE | td> | ||||||||||||||
354 | 922764 | BI1984 | 09-17 | O194 | pSBX4C5-1GateSspDnaX-S11C HN->AG_H6 | 40 | 1.90 | 18.10 | 20 | VF | ok | pSBX4C5-1GateSspDnaX-S11C HN->AG_H6 - O194 | attaccgcctttgagtgagc | |||||||||||||||
355 | 922764 | BI1986 | 09-17 | O195 | pSBX4C5-1GateSspDnaX-S11N_H6 | 40 | 2.16 | 17.84 | 20 | VR | ok | Jan | pSBX4C5-1GateSspDnaX-S11N_H6 - O195 | #N/A | ||||||||||||||
356 | 922764 | BI1988 | 09-17 | O196 | pSBX4C5-1GateSspDnaX-S11N C->G_H6 | 40 | 2.05 | 17.95 | 20 | VR | ok | pSBX4C5-1GateSspDnaX-S11N C->G_H6 - O196 | #N/A | |||||||||||||||
357 | 922764 | BI1990 | 09-17 | O197 | pSBX4C5-1GateSspGyrB-S11C_H6 | 40 | 1.96 | 18.04 | 20 | VF | ok | pSBX4C5-1GateSspGyrB-S11C_H6 - O197 | #N/A | |||||||||||||||
358 | 922764 | BI1992 | 09-17 | O198 | pSBX4C5-1GateSspGyrB-S11C SN->AG_H6 | 40 | 2.20 | 17.80 | 20 | VF | ok | Jan | pSBX4C5-1GateSspGyrB-S11C SN->AG_H6 - O198 td> | #N/A | ||||||||||||||
359 | 922764 | BI1994 | 09-17 | O199 | pSBX4C5-1GateSspGyrB-S11N_H6 | 40 | 2.42 | 17.58 | 20 | VR | ok | pSBX4C5-1GateSspGyrB-S11N_H6 - O199 | #N/A | |||||||||||||||
360 | 922764 | BI1996 | 09-17 | O200 | pSBX4C5-1GateSspGyrB-S11N C->G_H6 | 40 | 2.56 | 17.44 | 20 | VR | ok | pSBX4C5-1GateSspGyrB-S11N C->G_H6 - O200 | #N/A | |||||||||||||||
361 | 922764 | BI1998 | 09-17 | O201 | pSBX4C5-1GateTerDnaE3-S11C_H6 | 40 | 1.85 | 18.15 | 20 | VF | ok | pSBX4C5-1GateTerDnaE3-S11C_H6 - O201 | #N/A | |||||||||||||||
362 | 922764 | BI2000 | 09-17 | O202 | pSBX4C5-1GateTerDnaE3-S11C SN->AG_H6 | 40 | 2.11 | 17.89 | 20 | VF | ok | Jan | pSBX4C5-1GateTerDnaE3-S11C SN->AG_H6 - O202 | #N/A | ||||||||||||||
363 | 922764 | BI2002 | 09-17 | O203 | pSBX4C5-1GateTerDnaE3-S11(N)-H6 | 40 | 1.74 | 18.26 | 20 | VR | ok | pSBX4C5-1GateTerDnaE3-S11(N)-H6 - O203 | tgccacctgacgtctaagaa | |||||||||||||||
364 | 922764 | BI2004 | 09-17 | O204 | pSBX4C5-1GateTerDnaE3-S11(N)*-H6 | 40 | 2.00 | 18.00 | 20 | VR | ok | pSBX4C5-1GateTerDnaE3-S11(N)*-H6 - O204 | tgccacctgacgtctaagaa | |||||||||||||||
365 | 922764 | BI2006 | 09-17 | O191 | pSBX4C5-1GateNpuDnaE(C)*-H6 | 40 | 2.55 | 17.45 | 20 | VF | looks like pSBX4C5-1GateNpuDnaEN_C->G_H6 | Jan | pSBX4C5-1GateNpuDnaE(C)*-H6 - O191 | tgccacctgacgtctaagaa | ||||||||||||||
366 | 922764 | BI2008 | 09-17 | O191-2 | pSBX4C5-1GateNpuDnaE(C)*-H6 | 40 | 2.60 | 17.40 | 20 | VF | looks like pSBX4C5-1GateNpuDnaEN_C->G_H6 | Jan | pSBX4C5-1GateNpuDnaE(C)*-H6 - O191-2 | tgccacctgacgtctaagaa | ||||||||||||||
367 | Bl2010 | 9/18/2014 | O180-3 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 17.35 | 5.00 | 15.00 | 20 | GATC-VR-980199 | VR | mutation in first stop codon as well, mutation in chromophore region, no C but S | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-3 | FALSE | |||||||||||||||
368 | Bl2011 | 9/18/2014 | O180-4 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 42.6375 | 5.00 | 15.00 | 20 | pSBX74C5-mCherry_contr | #N/A | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-4 | FALSE | ||||||||||||||||
369 | Bl1963 | O210-2 | pSB1C3-BsaI-CC-O-IasB-Ssra | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | pSB1C3-BsaI-CC-O-IasB-Ssra - O210-2 | attaccgcctttgagtgagc | ||||||||||||||||||
370 | AG3770 | O211-2 | pSB1C3-BsaI-CC-O-IasB-MTS | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | pSB1C3-BsaI-CC-O-IasB-MTS - O211-2 | attaccgcctttgagtgagc | ||||||||||||||||||
371 | AG3771 | O212-2 | pSB1C3-EX-BsaI-A-NN-IasB-myristoilation | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | pSB1C3-EX-BsaI-A-NN-IasB-myristoilation - O212-2 | FALSE | ||||||||||||||||||
372 | AG3772 | O213-2 | pSB1C3-EX-BsaI-A-NN-IasB-NLS | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | pSB1C3-EX-BsaI-A-NN-IasB-NLS - O213-2 | FALSE | td> | |||||||||||||||||
373 | AG3773 | O214-2 | pSB1C3-EX-BsaI-A-NN-IasB-pelB | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | pSB1C3-EX-BsaI-A-NN-IasB-pelB - O214-2 | attaccgcctttgagtgagc | ||||||||||||||||||
374 | O215-2 | pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES - O215-2 | attaccgcctttgagtgagc | |||||||||||||||||||
375 | O218-2 | pSB1A2-BBa_K103006 | 40 | 8.93 | 11.07 | 20 | #N/A | pSB1A2-BBa_K103006 - O218-2 | FALSE | |||||||||||||||||||
376 | BI1985 | 09-19 | O175 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 4.13 | 15.87 | 20 | VF2 | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175 | FALSE | ||||||||||||||||
377 | BI1987 | 09-19 | O175-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 5.69 | 14.31 | 20 | VF2 td> | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-2 | attaccgcctttgagtgagc | ||||||||||||||||
378 | BI1989 | 09-19 | O175-3 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 3.84 | 16.16 | 20 | VF2 | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-3 | attaccgcctttgagtgagc | ||||||||||||||||
379 | BI1991 | 09-19 | O175-4 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 7.88 | 12.12 | 20 | VF2 | really is BsmBI version; ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-4 | FALSE | ||||||||||||||||
380 | BI1993 | 09-19 | O175-5 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 3.24 | 16.76 | 20 | VF2 | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-5 | FALSE | ||||||||||||||||
381 | BI1995 | 09-19 | O175-6 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 2.34 | 17.66 | 20 | VF2 | really is BsmBI version; ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-6 | tgccacctgacgtctaagaa | ||||||||||||||||
382 | BI1997 | 09-19 | O175-7 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 9.05 | 10.95 | 20 | VF2 | really is BsmBI version; ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-7 | tgccacctgacgtctaagaa | ||||||||||||||||
383 | BI1999 | 09-19 | O190 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6 | 40 | 2.89 | 17.11 | 20 | VF2 | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6 - O190 | tgccacctgacgtctaagaa | ||||||||||||||||
384 | BI2001 | 09-19 | O190-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6 | 40 | 10.71 | 9.29 | 20 | VF2 | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6 - O190-2 | td> | tgccacctgacgtctaagaa | |||||||||||||||
385 | BI2003 | 09-19 | O175 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 4.13 | 15.87 | 20 | VR | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175 | attaccgcctttgagtgagc | ||||||||||||||||
386 | BI2005 | 09-19 | O175-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 5.69 | 14.31 | 20 | VR | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-2 | attaccgcctttgagtgagc | ||||||||||||||||
387 | BI2007 | 09-19 | O175-3 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 3.84 | 16.16 | 20 | VR | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-3 | attaccgcctttgagtgagc | ||||||||||||||||
388 | BI2009 | 09-19 | O175-4 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 7.88 | 12.12 | 20 | VR | really is BsmBI version; ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-4 | attaccgcctttgagtgagc | ||||||||||||||||
389 | AF9233 | 09-19 | O175-5 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 3.24 | 16.76 | 20 | VR | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-5 | attaccgcctttgagtgagc | ||||||||||||||||
390 | AF9234 | 09-19 | O175-6 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 2.34 | 17.66 | 20 | VR | really is BsmBI version; ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-6 | attaccgcctttgagtgagc | ||||||||||||||||
391 | AF9235 | 09-19 | O175-7 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 40 | 9.05 | 10.95 | 20 | VR | really is BsmBI version; ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O175-7 | attaccgcctttgagtgagc | ||||||||||||||||
392 | AF9236 | 09-19 | O190 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6 | 40 | 2.89 | 17.11 | 20 | VR | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6 - O190 | attaccgcctttgagtgagc | ||||||||||||||||
393 | II2292 | 09-19 | O190-2 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6 | 40 | 10.71 | 9.29 | 20 | VR | ok | pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-CGCA=BsmBI-mRFPexp-BsmBI=CTTC)-H6 - O190-2 | attaccgcctttgagtgagc | ||||||||||||||||
394 | BF8258 | 09-22 | O180-5 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 6.72 | 13.28 | 20 | VR | no mutation has been introduced and frame shift in stop codon | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-5 | attaccgcctttgagtgagc | ||||||||||||||||
395 | BF8259 | 09-22 | O180-6 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 5.71 | 14.29 | 20 | VR | ok good | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-6 td> | attaccgcctttgagtgagc | ||||||||||||||||
396 | BF8260 | 09-22 | O180-7 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 5.44 | 14.56 | 20 | VR | no mutation has been introduced and frame shift in stop codon | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-7 | attaccgcctttgagtgagc | ||||||||||||||||
397 | BF8261 | 09-22 | O220 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.77 | 18.23 | 20 | VR | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220 | attaccgcctttgagtgagc | |||||||||||||||||
398 | BF8262 | 09-22 | O220 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.77 | 18.23 | 20 | VF | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220 | FALSE | |||||||||||||||||
399 | BF8263 | 09-22 | O220-2 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 | 18.10 | 20 | VR | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-2 | attaccgcctttgagtgagc | td> | ||||||||||||||||
400 | BF8264 | 09-22 | O220-2 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 | 18.10 | 20 | VF | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-2 | FALSE | |||||||||||||||||
401 | BF8265 | 09-22 | O220-3 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 | 18.10 | 20 | VR | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-3 | attaccgcctttgagtgagc | |||||||||||||||||
402 | BF8266 | 09-22 | O220-3 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 | 18.10 | 20 | VF | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-3 | FALSE | |||||||||||||||||
403 | BF8267 | 09-22 | O223 | pSBX4C5-0GateSspDnaX-S11C_mRFP169-225 | 40 | 1.48 | 18.52 | 20 | VF | ok good | pSBX4C5-0GateSspDnaX-S11C_mRFP169-225 - O223 | FALSE | ||||||||||||||||
404 | BF8268 | 09-22 | O224 | pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 | 40 | 1.69 | 18.31 | 20 | VR | ok good | pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 - O224 | attaccgcctttgagtgagc | ||||||||||||||||
405 | BF8269 | 09-22 | O224-2 | pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 | 40 | 6.50 | 13.50 | 20 | VR | ok good | pSBX4C5-0GateSspDnaX-S11N_mRFP1-168 - O224-2 | attaccgcctttgagtgagc | ||||||||||||||||
406 | BF8270 | 09-22 | O225 | pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 | 40 | 1.40 | 18.60 | 20 | VF | voller mutationen -> falsches FP insert? NEU, use O225-2 | pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 - O225 | FALSE | ||||||||||||||||
407 | BF8271 | 09-22 | O226 | pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 | 40 | 2.63 | 17.37 | 20 | VR | frameshift mutation? NEU,useO226-4 | pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 - O226 | attaccgcctttgagtgagc | ||||||||||||||||
408 | BF8272 | 09-22 | O227 | pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 | 40 | 1.47 | 18.53 | 20 | VF | kein insert? NEU | pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 - O227 | FALSE | ||||||||||||||||
409
th> | BF8273 | 09-22 | O228 | pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 | 40 | 0.91 | 19.09 | 20 | VR | mutation im prefix? but less mutation than O228-2,use O228-4 | pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 - O228 | attaccgcctttgagtgagc | ||||||||||||||||
410 | BF8274 | 09-22 | O228-2 | pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 | 40 | 2.90 | 17.10 | 20 | VR | mutation im prefix? | pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 - O228-2 | attaccgcctttgagtgagc | ||||||||||||||||
411 | BF8275 | 09-22 | O229 | pSBX4C5-0GateNpuDnaE_C_mRFP169-225 | 40 | 1.53 | 18.47 | 20 | VF | OK good | pSBX4C5-0GateNpuDnaE_C_mRFP169-225 - O229 | FALSE | ||||||||||||||||
412 | BF8276 | 09-22 | O230 | pSBX4C5-0GateNpuDnaE_N_mRFP1-168 | 40 | 2.67 | 17.33 | 20 | VR | mutation in mRFP G toD | pSBX4C5-0GateNpuDnaE_N_mRFP1-168 - O230 | attaccgcctttgagtgagc | ||||||||||||||||
413 | BF8277 | 09-22 | O191-3 | pSBX4C5-1GateNpuDnaE(C)*-H6 | 40 | 1.81 | 18.19 | 20 | VF | ok short sequencing | pSBX4C5-1GateNpuDnaE(C)*-H6 - O191-3 | FALSE | td> | |||||||||||||||
414 | BF8278 | 09-22 | O192-2 | pSBX4C5-1GateNpuDnaE(N)*-H6 | 40 | 2.04 | 17.96 | 20 | VR | ok short sequencing | pSBX4C5-1GateNpuDnaE(N)*-H6 - O192-2 | attaccgcctttgagtgagc | ||||||||||||||||
415 | BF8279 | 09-22 | O217 | pSBX1K3-XylaH6extBB | 40 | 4.67 | 15.33 | 20 | VR | bad seq | pSBX1K3-XylaH6extBB - O217 | attaccgcctttgagtgagc | ||||||||||||||||
416 | BF8280 | 09-22 | O217-2 | pSBX1K3-XylaH6extBB | 40 | 6.17 | 13.83 | 20 | VR | ok | pSBX1K3-XylaH6extBB - O217-2 | attaccgcctttgagtgagc | ||||||||||||||||
417 | BF8281 | 09-23 | O220 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.77 | 18.23 | 20 | 20287 | LOV_F_H6 | good, but wrong -> no mutation | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220 | FALSE | |||||||||||||||
418 | BF8282 | 09-23 | O220 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 td> | 18.10 | 20 | 20288 | LOV-12_R_SspDnaXC | good, but wrong -> no mutation | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-2 | FALSE | |||||||||||||||
419 | BF8283 | 09-23 | O220-2 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 | 18.10 | 20 | 20287 | LOV_F_H6 | ok, good! | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-3 | FALSE | |||||||||||||||
420 | BF8284 | 09-23 | O220-2 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 | 18.10 | 20 | 20288 | LOV-12_R_SspDnaXC | ok, good! | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-3 | FALSE | |||||||||||||||
421 | BF8285 | 09-23 | O220-3 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 | 18.10 | 20 | 20287 | LOV_F_H6 | ok, good! | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-3 | FALSE | |||||||||||||||
422 | BF8286 | 09-23 | O220-3 | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T | 40 | 1.90 | 18.10 | 20 | 20288 | LOV-12_R_SspDnaXC | ok, good! | pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T - O220-3 | FALSE | |||||||||||||||
423 | 20287 | 09-23 | LOV_F_H6 | 350 | #N/A | #N/A | #N/A | 20 | #N/A | LOV_F_H6 - 350 | #N/A | |||||||||||||||||
424 | 20288 | 09-23 | LOV-12_R_SspDnaXC | 330 | #N/A | #N/A | #N/A | 20 | #N/A | LOV-12_R_SspDnaXC - 330 | #N/A | |||||||||||||||||
425 | BF8287 | 09-23 | O225-2 | pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 | 40 | #N/A | #N/A | 20 | VF2 | ok,good | pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 - 350 | tgccacctgacgtctaagaa | ||||||||||||||||
426 | BF8288 | 09-23 | O225-3 | pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 | 40 | #N/A | #N/A | 20 | VF2 | no mRFPc insert | pSBX4C5-0GateSspGyrB-S11C_mRFP169-225 - 330 | tgccacctgacgtctaagaa | ||||||||||||||||
427 | BF8289 td> | 09-23 | O226-3 | pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 | 40 | #DIV/0! | #DIV/0! | 20 | VR | frame shift in beginning | pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 - O226-3 | attaccgcctttgagtgagc | ||||||||||||||||
428 | BF8290 | 09-23 | O226-4 | pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 | 40 | 1.82 | 18.18 | 20 | VR | ok, good | pSBX4C5-0GateSspGyrB-S11N_mRFP1-168 - O226-4 | attaccgcctttgagtgagc | ||||||||||||||||
429 | BF8291 | 09-23 | O227-2 | pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | no mRFPc insert | pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 - O227-2 | tgccacctgacgtctaagaa | ||||||||||||||||
430 | BF8292 | 09-23 | O227-3 | pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | no mRFPc insert | pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225 - O227-3 | tgccacctgacgtctaagaa | ||||||||||||||||
431 | BF8293 | 09-23 | O228-4 | pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168 | 40 | 1.21 | 18.79 | 20 | VF2 | ok, but one mutation in LacOperon | pSBX4C5-0GateTerDnaE3-S11N_ mRFP1-168 - O228-4 | tgccacctgacgtctaagaa | ||||||||||||||||
432 | BF8294 | 09-23 | O229-2 | pSBX4C5-0GateNpuDnaE_C_mRFP169-225 | 40 | #DIV/0! | #DIV/0! | 20 | VR | ok, good | pSBX4C5-0GateNpuDnaE_C_mRFP169-225 - O229-2 | attaccgcctttgagtgagc | ||||||||||||||||
433 | DD1384 | 09-24 | O232 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) | 43.386 | 6.00 | 14.00 | 20 | #N/A | ok | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) - O232 | #N/A | ||||||||||||||||
434 | DD1385 | 09-24 | O232-2 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) | 40.2 | 7.50 | 12.50 | 20 | #N/A | ok | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) - O232-2 | #N/A | ||||||||||||||||
435 | DD1386 | 09-24 | O231 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 39.48 | 10.00 | 10.00 | 20 | #N/A | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O231 | #N/A | td> | ||||||||||||||||
436 | DD1387 | 09-24 | O239-3 | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 | 39.816 | 18.00 | 2.00 | 20 | #N/A | no result | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 - O239-3 | #N/A | ||||||||||||||||
437 | DD1388 | 09-24 | O233-3 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 40.05 | 15.00 | 5.00 | 20 | #N/A | no result | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) - O233-3 | #N/A | ||||||||||||||||
438 | DD1389 | 09-24 | O262 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) | 40.29 | 5.00 | 15.00 | 20 | #N/A | no result | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) - O262 | #N/A | ||||||||||||||||
439 | DD1390 | 09-24 | O262-4 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) | 41.504 | 4.00 | 16.00 | 20 | #N/A | no result | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) - O262-4 | #N/A | ||||||||||||||||
440 | DD1391 | 09-24 | O232 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) | 20 | #N/A | ok | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) - O232 | #N/A | |||||||||||||||||||
441 | DD1392 | 09-24 | O232-2 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) | 20 | #N/A | frameshift mutation behind DNMT1 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT) - O232-2 | #N/A | |||||||||||||||||||
442 | DD1393 | 09-24 | O231 | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 | 20 | #N/A | pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6 - O231 | #N/A | ||||||||||||||||||||
443 | DD1394 | 09-24 | O239-3 | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 | 20 | #N/A | no result | pSBX1K3-T7RBS-2ZSortA:eGFP-H6 - O239-3 | #N/A | |||||||||||||||||||
444 | DD1395 | 09-24 | O233-3 | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) | 20 | #N/A | no result | pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP) - O233-3 | #N/A | |||||||||||||||||||
445
div> | DD1396 | 09-24 | O262 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) | 20 | #N/A | no result | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) - O262 | #N/A | |||||||||||||||||||
446 | DD1397 | 09-24 | O262-4 | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) | 20 | #N/A | no result | pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT) - O262-4 | #N/A | |||||||||||||||||||
447 | BF8295 | 09-24 | O260 | pSBX1K3-BBa_I13507-GSGIDECHT | 40 | 10.00 | 10.00 | 20 | VR | insertion, caused by mutated template that was not checked correctly by Jan :-P | Jakob | pSBX1K3-BBa_I13507-GSGIDECHT - O260 | attaccgcctttgagtgagc | |||||||||||||||
448 | BF8296 | 09-24 | O260-2 | pSBX1K3-BBa_I13507-GSGIDECHT | 40 | 10.00 | 10.00 | 20 | VR | insertion Jan | Jakob | pSBX1K3-BBa_I13507-GSGIDECHT - O260-2 | attaccgcctttgagtgagc | td> | ||||||||||||||
449 | BF8297 | 09-24 | O261 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 40 | 10.00 | 10.00 | 20 | VR | insertion | Jakob | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha - O261 | attaccgcctttgagtgagc | |||||||||||||||
450 | DD1398 | 09-24 | O261-2 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 40 | 10.00 | 10.00 | 20 | VR | insertion | Jakob | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha - O261-2 | attaccgcctttgagtgagc | |||||||||||||||
451 | DD1399 | 09-24 | O260-3 | pSBX1K3-BBa_I13507-GSGIDECHT | 40 | 10.00 | 10.00 | 20 | VR | insertion | Jakob | pSBX1K3-BBa_I13507-GSGIDECHT - O260-3 | attaccgcctttgagtgagc | |||||||||||||||
452 | DD1400 | 09-24 | O260-4 | pSBX1K3-BBa_I13507-GSGIDECHT | 40 | 10.00 | 10.00 | 20 | VR | insertion | Jakob | pSBX1K3-BBa_I13507-GSGIDECHT - O260-4 | attaccgcctttgagtgagc | |||||||||||||||
453 | DD1401 | 09-24 | O255 | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 | 40 | 10.00 | 10.00 | 20 | #N/A | no | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 - O255 | #N/A | ||||||||||||||||
454 | DD1402 | 09-24 | O257 | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 - O257 | #N/A | ||||||||||||||||
455 | DD1403 | 09-24 | O259 | pSBX4C5-1GateNpuDnaE_mutated_coex | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-1GateNpuDnaE_mutated_coex - O259 | #N/A | ||||||||||||||||
456 | DD1404 | 09-24 | O255-2 | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 - O255-2 | #N/A | ||||||||||||||||
457 | DD1405 | 09-24 | O257-2 | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 - O257-2 | #N/A | ||||||||||||||||
458 | BI2146 | O255 td> | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 - O255 | #N/A | |||||||||||||||||
459 | BI2194 | O257 | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 - O257 | #N/A | |||||||||||||||||
460 | BI2195 | O259 | pSBX4C5-1GateNpuDnaE_mutated_coex | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-1GateNpuDnaE_mutated_coex - O259 | #N/A | |||||||||||||||||
461 | DD1406 | O255-2 | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 - O255-2 | #N/A | |||||||||||||||||
462 | DD1407 | O257-2 | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 | 40 | #DIV/0! | #DIV/0! | 20 | #N/A | no | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 - O257-2 | #N/A | |||||||||||||||||
463 | BI2012 | 9/18/2014 | O209-4 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
div> | 30.575 | 5.00 | 15.00 | 20 | VF2 | ok | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) - O209-4 | tgccacctgacgtctaagaa | ||||||||||||||||
464 | BI2013 | 9/18/2014 | O209-6 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 5.00 | 15.00 | 20 | VF2 | really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector) | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) - O209-6 | tgccacctgacgtctaagaa | |||||||||||||||||
465 | BI2014 | 9/18/2014 | O209-8 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 12.45 | 5.00 | 15.00 | 20 | VF2 | no result (concentration probably was too low) | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) - O209-8 | tgccacctgacgtctaagaa | ||||||||||||||||
466 | AG3764 | 9/18/2014 | O176 | pSB1C3-H6-Smt3-0ZSortA | 17.125 | 5.00 | 15.00 | 20 | VF2 | ok | pSB1C3-H6-Smt3-0ZSortA - O176 | tgccacctgacgtctaagaa | tr> | |||||||||||||||
467 | AG3765 | 9/18/2014 | O176-2 | pSB1C3-H6-Smt3-0ZSortA | 5.00 | 15.00 | 20 | VF2 | H6R mutation | pSB1C3-H6-Smt3-0ZSortA - O176-2 | tgccacctgacgtctaagaa | |||||||||||||||||
468 | AG3766 | 9/18/2014 | O176-3 | pSB1C3-H6-Smt3-0ZSortA | 21.95 | 5.00 | 15.00 | 20 | VF2 | ok | pSB1C3-H6-Smt3-0ZSortA - O176-3 | tgccacctgacgtctaagaa | ||||||||||||||||
469 | IH6892 | 9/18/2014 | O209-4 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 30.575 | 5.00 | 15.00 | 20 | VR | ccdB missing | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) - O209-4 | attaccgcctttgagtgagc | ||||||||||||||||
470 | IH6893 | 9/18/2014 | O209-6 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 5.00 | 15.00 | 20 | VR | really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector) | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) - O209-6 | attaccgcctttgagtgagc | |||||||||||||||||
471 | IH6894 | 9/18/2014 | O209-8 | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) | 12.45 | 5.00 | 15.00 | 20 | VR | no result (concentration probably was too low) | pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK) - O209-8 | attaccgcctttgagtgagc | ||||||||||||||||
472 | IH6895 | 9/18/2014 | O176 | pSB1C3-H6-Smt3-0ZSortA | 17.125 | 5.00 | 15.00 | 20 | VR | ok | pSB1C3-H6-Smt3-0ZSortA - O176 | attaccgcctttgagtgagc | ||||||||||||||||
473 | IH6896 | 9/18/2014 | O176-2 | pSB1C3-H6-Smt3-0ZSortA | 5.00 | 15.00 | 20 | VR | ok | pSB1C3-H6-Smt3-0ZSortA - O176-2 | attaccgcctttgagtgagc | |||||||||||||||||
474 | IH6897 | 9/18/2014 | O176-3 | pSB1C3-H6-Smt3-0ZSortA | 21.95 | 5.00 | 15.00 | 20 | VR | ok | pSB1C3-H6-Smt3-0ZSortA - O176-3 | attaccgcctttgagtgagc | ||||||||||||||||
475 | AG3767 | 9/18/2014 | O178 | pSB1C3-H6-0ZSortA | 64.75 | 5.00 | 15.00 | 20 | VR | ok | pSB1C3-H6-0ZSortA - O178 | attaccgcctttgagtgagc | ||||||||||||||||
476 | AG3768 | 9/18/2014 | O178-2 | pSB1C3-H6-0ZSortA | 69 | 5.00 | 15.00 | 20 | VR | ok | pSB1C3-H6-0ZSortA - O178-2 | attaccgcctttgagtgagc | ||||||||||||||||
477 | AG3769 | 9/18/2014 | O178-3 | pSB1C3-H6-0ZSortA | 71.875 | 5.00 | 15.00 | 20 | VR | ok | pSB1C3-H6-0ZSortA - O178-3 | attaccgcctttgagtgagc | ||||||||||||||||
478 | DD1408 | 9/25/2014 | O180-8 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 2.26 | 17.74 | 20 | VF2 | no mutagenesis | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-8 | tgccacctgacgtctaagaa | ||||||||||||||||
479 | DD1409 | 9/25/2014 | O180-9 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 4.08 | 15.92 | 20 | VF2 | no mutagenesis td> | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-9 | tgccacctgacgtctaagaa | ||||||||||||||||
480 | DD1410 | 09-25 | O180-10 | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 | 40 | 4.42 | 15.58 | 20 | VF2 | OK, mutagenesis worked | pSBX1K3-T7RBS-H6-sfGFP_T65C-H6 - O180-10 | tgccacctgacgtctaagaa | ||||||||||||||||
481 | DD1411 | 09-26 | O263-2 | pSB1C3-EX-BsaI-A-NN-IasB-OmpA | 40 | 3.45 | 16.55 | 20 | VF2 | pSB1C3-EX-BsaI-A-NN-IasB-OmpA - O263-2 | tgccacctgacgtctaagaa | |||||||||||||||||
482 | DD1412 | 09-26 | O263-2 | pSB1C3-EX-BsaI-A-NN-IasB-OmpA | 40 | 3.45 | 16.55 | 20 | VR | pSB1C3-EX-BsaI-A-NN-IasB-OmpA - O263-2 | attaccgcctttgagtgagc | |||||||||||||||||
483 | DD1413 | 09-29 | O278 | pSBX1K3-DnaXN-sfGFPn-H6 | 40 | 2.07 | 17.93 | 20 | VR | ok one additional His | pSBX1K3-DnaXN-sfGFPn-H6 - O278 | attaccgcctttgagtgagc | ||||||||||||||||
484 | DD1414 | 09-29 | O278-2 | pSBX1K3-DnaXN-sfGFPn-H6 | 40 | 1.99 | 18.01 | 20 | VR | ok two additional His | pSBX1K3-DnaXN-sfGFPn-H6 - O278-2 | attaccgcctttgagtgagc | ||||||||||||||||
485 | DD1415 | 09-29 | O277 | pSBX1K3-DnaXN*-sfGFPn-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | ok one additional His+ | pSBX1K3-DnaXN*-sfGFPn-H6 - O277 | attaccgcctttgagtgagc | ||||||||||||||||
486 | DD1416 | 09-29 | O277-2 | pSBX1K3-DnaXN*-sfGFPn-H6 | 40 | 3.81 | 16.19 | 20 | VR | okay | pSBX1K3-DnaXN*-sfGFPn-H6 - O277-2 | attaccgcctttgagtgagc | ||||||||||||||||
487 | DD1417 | 09-29 | O276 | pSBX1K3-SspDnaXc-sfGFP^C-H6 | 40 | 6.84 | 13.16 | 20 | VR | ok? two additional His | pSBX1K3-SspDnaXc-sfGFP^C-H6 - O276 | attaccgcctttgagtgagc | ||||||||||||||||
488 | DD1418 | 09-29 | O276-2 | pSBX1K3-SspDnaXc-sfGFP^C-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | do not use! bad mutations | pSBX1K3-SspDnaXc-sfGFP^C-H6 - O276-2 | attaccgcctttgagtgagc | td> | |||||||||||||||
489 | DD1419 | 09-29 | O279 | pSBX1K3-LOV-BsaIIasB-sfGFPc | 40 | 1.73 | 18.27 | 20 | VR | could be O281?? checking with next sequencing, changed sequencing or construct, additional His | pSBX1K3-LOV-BsaIIasB-sfGFPc - O279 | attaccgcctttgagtgagc | ||||||||||||||||
490 | DD1420 | 09-29 | O279-2 | pSBX1K3-LOV-BsaIIasB-sfGFPc | 40 | #DIV/0! | #DIV/0! | 20 | VR | could be O281?? checking with next sequencing, changed sequencing or construct, additional His | pSBX1K3-LOV-BsaIIasB-sfGFPc - O279-2 | attaccgcctttgagtgagc | ||||||||||||||||
491 | DD1421 | 09-29 | O280 | pSBX1K3-LOV-12-DnaXC-sfGFPc | 40 | #DIV/0! | #DIV/0! | 20 | VR | no LOV domain | pSBX1K3-LOV-12-DnaXC-sfGFPc - O280 | attaccgcctttgagtgagc | ||||||||||||||||
492 | DD1422 | 09-29 | O280-2 | pSBX1K3-LOV-12-DnaXC-sfGFPc | 40 | #DIV/0! | #DIV/0! | 20 | VR | one additional His, frame shift in the end | pSBX1K3-LOV-12-DnaXC-sfGFPc - O280-2 | attaccgcctttgagtgagc | ||||||||||||||||
493 | DD1423 | 09-29 | O281 | pSBX1K3-LOV-6-DnaXC-sfGFPc | 40 | 1.69 | 18.31 | 20 | VR | could be O279?? checking with next sequencing, changed sequencing or construct, additional His | pSBX1K3-LOV-6-DnaXC-sfGFPc - O281 | attaccgcctttgagtgagc | ||||||||||||||||
494 | DD1424 | 09-29 | O281-2 | pSBX1K3-LOV-6-DnaXC-sfGFPc | 40 | #DIV/0! | #DIV/0! | 20 | VR | could be O279?? checking with next sequencing, changed sequencing or construct, additional His | pSBX1K3-LOV-6-DnaXC-sfGFPc - O281-2 | attaccgcctttgagtgagc | ||||||||||||||||
495 | DD1425 | 09-29 | O267 | pSBX1K3-H6-mRFPn-DnaXN-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | bad read at the beginning, probably frame shift mutations in between | pSBX1K3-H6-mRFPn-DnaXN-H6 - O267 | attaccgcctttgagtgagc | ||||||||||||||||
496 | DD1426 | 09-29 | O267-2 | pSBX1K3-H6-mRFPn-DnaXN-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | turned out to be pSBX1K3-sfGFPn-NpuDnaEN | pSBX1K3-H6-mRFPn-DnaXN-H6 - O267-2 | attaccgcctttgagtgagc | ||||||||||||||||
497 | DD1427 | 09-29 | O268 | pSBX1K3-H6-mRFPn-DnaXN*-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | Intein DnaXN insert not included | pSBX1K3-H6-mRFPn-DnaXN*-H6 - O268 | attaccgcctttgagtgagc | ||||||||||||||||
498 | DD1428 | 09-29 | O268-2 | pSBX1K3-H6-mRFPn-DnaXN*-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | Intein DnaXN insert not included | pSBX1K3-H6-mRFPn-DnaXN*-H6 - O268-2 | attaccgcctttgagtgagc | ||||||||||||||||
499 | DD1429 | 09-29 | O269 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | frame shift between His | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 - O269 | attaccgcctttgagtgagc | ||||||||||||||||
500 | DD1430 | 09-29 | O269-2 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 | 40 | 3.13 | 16.88 | 20 | VR | ok one additional His | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 - O269-2 | attaccgcctttgagtgagc | ||||||||||||||||
501 | DD1431 | 09-29 | O270 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 | 40 | 3.08 | 16.92 | 20 | VR | okay | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 - O270 | attaccgcctttgagtgagc | ||||||||||||||||
502 | DD1432 | 09-29 | O270-2 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | okay, one point mutation in Lac Operator | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 - O270-2 | attaccgcctttgagtgagc | ||||||||||||||||
503 | DD1433 | 09-29 | O271 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | 6 amino acids missing in helixintein construct | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 - O271 | attaccgcctttgagtgagc | ||||||||||||||||
504 | DD1434 | 09-29 | O271-2 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VR | LOV domain missing | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 - O271-2 | attaccgcctttgagtgagc | ||||||||||||||||
505 | DD1435 | 09-29 | O279 | pSBX1K3-LOV-BsaIIasB-sfGFPc | 40 | 1.73 | 18.27 | 20 | VF1 | could be O281?? checking with next sequencing | pSBX1K3-LOV-BsaIIasB-sfGFPc - O279 | FALSE | ||||||||||||||||
506 | DD1436 | 09-29 | O279-2 | pSBX1K3-LOV-BsaIIasB-sfGFPc | 40 td> | #DIV/0! | #DIV/0! | 20 | VF2 | could be O281?? checking with next sequencing, changed sequencing or construct, additional His | pSBX1K3-LOV-BsaIIasB-sfGFPc - O279-2 | tgccacctgacgtctaagaa | ||||||||||||||||
507 | DD1437 | 09-29 | O280 | pSBX1K3-LOV-12-DnaXC-sfGFPc | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | no LOV domain | pSBX1K3-LOV-12-DnaXC-sfGFPc - O280 | tgccacctgacgtctaagaa | ||||||||||||||||
508 | DD1438 | 09-29 | O280-2 | pSBX1K3-LOV-12-DnaXC-sfGFPc | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | one additional His, frame shift in the end | pSBX1K3-LOV-12-DnaXC-sfGFPc - O280-2 | tgccacctgacgtctaagaa | ||||||||||||||||
509 | DD1439 | 09-29 | O281 | pSBX1K3-LOV-6-DnaXC-sfGFPc | 40 | 1.69 | 18.31 | 20 | VF2 | could be O279?? checking with next sequencing, changed sequencing or construct, additional His | pSBX1K3-LOV-6-DnaXC-sfGFPc - O281 | tgccacctgacgtctaagaa | ||||||||||||||||
510 | DD1440 | 09-29 | O281-2 | pSBX1K3-LOV-6-DnaXC-sfGFPc | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | could be O279?? checking with next sequencing, changed sequencing or construct, additional His | pSBX1K3-LOV-6-DnaXC-sfGFPc - O281-2 | tgccacctgacgtctaagaa | ||||||||||||||||
511 | DD1441 | 09-29 | O269 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 - O269 | tgccacctgacgtctaagaa | |||||||||||||||||
512 | DD1442 | 09-29 | O269-2 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 | 40 | 3.13 | 16.88 | 20 | VF2 | pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6 - O269-2 | tgccacctgacgtctaagaa | |||||||||||||||||
513 | DD1443 | 09-29 | O270 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 | 40 | 3.08 | 16.92 | 20 | VF2 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 - O270 | tgccacctgacgtctaagaa | |||||||||||||||||
514 | DD1444 | 09-29 | O270-2 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6 - O270-2 | tgccacctgacgtctaagaa | |||||||||||||||||
515 | DD1445 | 09-29 | O271 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 - O271 | tgccacctgacgtctaagaa | |||||||||||||||||
516 | DD1446 | 09-29 | O271-2 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 40 | #DIV/0! | #DIV/0! | 20 | VF2 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 - O271-2 | tgccacctgacgtctaagaa | |||||||||||||||||
517 | DD1447 | 10-01 | O327 | pSBX4C5-1GateGp41-1(N)-H6 | 40 | 20.00 | 0.00 | 20 | GATC-VR-980199 | VR | ok | pSBX4C5-1GateGp41-1(N)-H6 - O327 | attaccgcctttgagtgagc | |||||||||||||||
518 | DD1448 | 10-01 | O327-2 | pSBX4C5-1GateGp41-1(N)-H6 | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | will be repeated | pSBX4C5-1GateGp41-1(N)-H6 - O327-2 | attaccgcctttgagtgagc | |||||||||||||||
519 | DD1449 | 10-01 | O328 | pSBX4C5-H6-1GateGp41-1(C) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | ok | pSBX4C5-H6-1GateGp41-1(C) - O328 | tgccacctgacgtctaagaa | |||||||||||||||
520 | DD1450 | 10-01 | O328-2 | pSBX4C5-H6-1GateGp41-1(C) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | ok | pSBX4C5-H6-1GateGp41-1(C) - O328-2 | tgccacctgacgtctaagaa | |||||||||||||||
521 | DD1451 | 10-01 | O329 | pSBX4C5-DoubleGateGp41-1(N) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | ok, not completely sequenced | pSBX4C5-DoubleGateGp41-1(N) - O329 | tgccacctgacgtctaagaa | |||||||||||||||
522 | DD1452 | 10-01 | O329-2 | pSBX4C5-DoubleGateGp41-1(N) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | bad sequenzing | pSBX4C5-DoubleGateGp41-1(N) - O329-2 | tgccacctgacgtctaagaa | |||||||||||||||
523 | DD1453 | 10-01 | O330 | pSBX4C5-DoubleGateGp41-1(C) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | ok, not completely sequenced | pSBX4C5-DoubleGateGp41-1(C) - O330 | tgccacctgacgtctaagaa | |||||||||||||||
524 | DD1454 | 10-01 | O330-2 | pSBX4C5-DoubleGateGp41-1(C) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | insertion in the prefix, mutation in mRFP | pSBX4C5-DoubleGateGp41-1(C) - O330-2 | tgccacctgacgtctaagaa | |||||||||||||||
525 | DD1455 | 10-01 | O331 | pSBX4C5-DoubleNpuDnaE(N) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | sequencing not long enough | pSBX4C5-DoubleNpuDnaE(N) - O331 | tgccacctgacgtctaagaa | |||||||||||||||
526 | DD1456 | 10-01 | O331-2 | pSBX4C5-DoubleNpuDnaE(N) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | sequencing not long enough | pSBX4C5-DoubleNpuDnaE(N) - O331-2 | tgccacctgacgtctaagaa | |||||||||||||||
527 | DD1457 | 10-01 | O332 | pSBX4C5-DoubleGateNpuDnaE(C) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | sequencing not long enough, intein not sequenced | pSBX4C5-DoubleGateNpuDnaE(C) - O332 | tgccacctgacgtctaagaa | |||||||||||||||
528 | DD1458 | 10-01 | O332-2 | pSBX4C5-DoubleGateNpuDnaE(C) | 40 | #N/A | 0.00 | 20 | GATC-VF2-980198 | VF2 | poor sequenzing, especially in the beginningg, but no indication that it was not ok | pSBX4C5-DoubleGateNpuDnaE(C) - O332-2 | tgccacctgacgtctaagaa | |||||||||||||||
529 | DD1459 | 10-01 | O329 | pSBX4C5-DoubleGateGp41-1(N) | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | ok, not completely sequenced | pSBX4C5-DoubleGateGp41-1(N) - O329 | attaccgcctttgagtgagc | |||||||||||||||
530 | DD1460 | 10-01 | O329-2 | pSBX4C5-DoubleGateGp41-1(N) | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | bad sequenzing | pSBX4C5-DoubleGateGp41-1(N) - O329-2 | attaccgcctttgagtgagc | |||||||||||||||
531 | DD1461 | 10-01 | O330 | pSBX4C5-DoubleGateGp41-1(C) td> | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | ok, not completely sequenced | pSBX4C5-DoubleGateGp41-1(C) - O330 | attaccgcctttgagtgagc | |||||||||||||||
532 | DD1462 | 10-01 | O330-2 | pSBX4C5-DoubleGateGp41-1(C) | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | poor sequenzing, but ok | pSBX4C5-DoubleGateGp41-1(C) - O330-2 | attaccgcctttgagtgagc | |||||||||||||||
533 | DD1463 | 10-01 | O331 | pSBX4C5-DoubleNpuDnaE(N) | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | poor sequenzing could be ok intein not sequenced | pSBX4C5-DoubleNpuDnaE(N) - O331 | attaccgcctttgagtgagc | |||||||||||||||
534 | DD1464 | 10-01 | O331-2 | pSBX4C5-DoubleNpuDnaE(N) | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | ok intein not sequenzed | pSBX4C5-DoubleNpuDnaE(N) - O331-2 | attaccgcctttgagtgagc | |||||||||||||||
535 | DD1465 | 10-01 | O332 | pSBX4C5- DoubleGateNpuDnaE(C) | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | short sequenzin but ok | pSBX4C5-DoubleGateNpuDnaE(C) - O332 | attaccgcctttgagtgagc | |||||||||||||||
536 | DD1466 | 10-01 | O332-2 | pSBX4C5-DoubleGateNpuDnaE(C) | 40 | #N/A | 0.00 | 20 | GATC-VR-980199 | VR | poor sequrnzing could be ok | pSBX4C5-DoubleGateNpuDnaE(C) - O332-2 | attaccgcctttgagtgagc | |||||||||||||||
537 | 20289 | 204 | ccdB-pSBX4C5-mRFP(N)_F | ccdB-pSBX4C5-mRFP(N)_F | ||||||||||||||||||||||||
538 | 20290 | 205 | ccdB-pSBX4C5-mRFP-(N)_R | ccdB-pSBX4C5-mRFP-(N)_R | ||||||||||||||||||||||||
539 | DD1467 | 10/2/2014 | O240 | pSBX4C5-2GateSspDnaB(C) | 40 | #N/A | #N/A | 20 | 20289 | 204 | ok | pSBX4C5-2GateSspDnaB(C) - O240 | FALSE | |||||||||||||||
540 | DD1468 | 10/2/2014 | O240-3 | pSBX4C5-2GateSspDnaB(C) | 40 | #N/A | #N/A | 20 | 20289 | 204 | ok (maybe there is something in the beginning of mRFP) | pSBX4C5-2GateSspDnaB(C) - O240-3 | FALSE | |||||||||||||||
541 | DD1469 | 10/2/2014 | O241-2 | pSBX4C5-2GateSspDnaB(C)* | 40 | #N/A | #N/A | 20 | 20289 | 204 | ok | pSBX4C5-2GateSspDnaB(C)* - O241-2 | FALSE | |||||||||||||||
542 | DD1470 | 10/2/2014 | O241-3 | pSBX4C5-2GateSspDnaB(C)* | 40 | #N/A | #N/A | 20 | 20289 | 204 | ok | pSBX4C5-2GateSspDnaB(C)* - O241-3 | FALSE | |||||||||||||||
543 | DD1471 | 10/2/2014 | O242 | pSBX4C5-2GateSspDnaB(N) | 40 | 6.31 | 13.69 | 20 | GATC-VR-980199 | VR | perfect mRFP read, call GATC | pSBX4C5-2GateSspDnaB(N) - O242 | attaccgcctttgagtgagc | |||||||||||||||
544 | DD1472 | 10/2/2014 | O242-2 | pSBX4C5-2GateSspDnaB(N) | 40 | 8.70 | 11.30 | 20 | GATC-VR-980199 | VR | perfect mRFP read, call GATC | pSBX4C5-2GateSspDnaB(N) - O242-2 | attaccgcctttgagtgagc | |||||||||||||||
545 | DD1473 | 10/2/2014 | O243-2 | pSBX4C5-2GateSspDnaB(N)* | 40 | 11.76 | 8.24 | 20 | GATC-VR-980199 | VR | perfect mRFP read, call GATC | pSBX4C5-2GateSspDnaB(N)* - O243-2 | attaccgcctttgagtgagc | |||||||||||||||
546 | DD1474 | 10/2/2014 | O243-3 | pSBX4C5-2GateSspDnaB(N)* | 40 | 16.00 | 4.00 | 20 | GATC-VR-980199 | VR | perfect mRFP read, call GATC | pSBX4C5-2GateSspDnaB(N)* - O243-3 | attaccgcctttgagtgagc | |||||||||||||||
547 | DD1475 | 10/2/2014 | O325 | pSBX4C5-2GateSspDnaE(N) | 40 | 10.00 | 10.00 | 20 | GATC-VR-980199 | VR | perfect mRFP read, call GATC | pSBX4C5-2GateSspDnaE(N) - O325 | attaccgcctttgagtgagc | |||||||||||||||
548 | DD1476 | 10/2/2014 | O325-2 | pSBX4C5-2GateSspDnaE(N) | 40 | 12.25 | 7.75 | 20 | GATC-VR-980199 | VR | perfect mRFP read, call GATC | pSBX4C5-2GateSspDnaE(N) - O325-2 | attaccgcctttgagtgagc | |||||||||||||||
549 | DD1477 | 10/2/2014 | O326 | pSBX4C5-2GateSspDnaE(C) | 40 | 8.45 | 11.55 | 20 | 20289 | 204 | double insert insertion | pSBX4C5-2GateSspDnaE(C) - O326 | FALSE | |||||||||||||||
550 | DD1478 | 10/2/2014 | O326-3 | pSBX4C5-2GateSspDnaE(C) | 40 | 8.82 | 11.18 | 20 | 20289 | 204 | tripple insert insertion | pSBX4C5-2GateSspDnaE(C) - O326-3 | FALSE | |||||||||||||||
551 | DD1479 | 10/2/2014 | O346 | pSB1C3-EX-BsaI-CBD | 40 | #DIV/0! | #DIV/0! | 20 | GATC-VR-980199 | VR | no.....GG could not work | pSB1C3-EX-BsaI-CBD - O346 | attaccgcctttgagtgagc | |||||||||||||||
552 | DD1480 | 10/2/2014 | O346-2 | pSB1C3-EX-BsaI-CBD | 40 | #DIV/0! | #DIV/0! | 20 | GATC-VR-980199 | VR | no.....GG could not work | pSB1C3-EX-BsaI-CBD - O346-2 | attaccgcctttgagtgagc | |||||||||||||||
553
div> | not ordered | DD1481 | 10/2/2014 | O364 | pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C) | 40 | 4.37 | 15.63 | 20 | #N/A | pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C) - O364 | #N/A | ||||||||||||||||
554 | not ordered | DD1482 | 10/2/2014 | O365 | pSBX4C5-1GateNpuDnaE(N)*-H6-1GateNpuDnaE(C)*-H6 | 40 | 3.84 | 16.16 | 20 | #N/A | pSBX4C5-1GateNpuDnaE(N)*-H6-1GateNpuDnaE(C)*-H6 - O365 | #N/A | ||||||||||||||||
555 | not ordered | DD1483 | 10/2/2014 | O366 | pSBX4C5-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2 | 40 | 3.98 | 16.02 | 20 | #N/A | pSBX4C5-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2 - O366 | #N/A | ||||||||||||||||
556 | O325 | pSBX4C5-2GateSspDnaE(N) | 40 | 10.00 | 10.00 | 20 | BAC1 | #N/A | pSBX4C5-2GateSspDnaE(N) - O325 | GCTCGTATGTTGTGTGGAATTG | ||||||||||||||||||
557 | O325-2 | pSBX4C5-2GateSspDnaE(N) | 40 | 12.25 | 7.75 | 20 | BAC1 | #N/A | pSBX4C5-2GateSspDnaE(N) - O325-2 | GCTCGTATGTTGTGTGGAATTG | ||||||||||||||||||
558 | DG7162 | O272-4 | pSBX1K3-H6-DnaXC-mRFPc-H6 | 40 | 4.76 | 15.24 | 20 | #N/A | pSBX1K3-H6-DnaXC-mRFPc-H6 - O272-4 | #N/A | ||||||||||||||||||
559 | DG7163 | O272-5 | pSBX1K3-H6-DnaXC-mRFPc-H6 | 40 | 4.23 | 15.77 | 20 | #N/A | pSBX1K3-H6-DnaXC-mRFPc-H6 - O272-5 | #N/A | ||||||||||||||||||
560 | DG7164 | O271-3 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 40 | 7.27 | 12.73 | 20 | #N/A | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 - O271-3 | #N/A | ||||||||||||||||||
561 | DG7165 | O271-4 | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 | 40 | 6.96 | 13.04 | 20 | #N/A | pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6 - O271-4 | #N/A | ||||||||||||||||||
562 | DG7166 | O275-4 | pSBX1K3-SspDnaXc*-sfGFP^C-H6 | 40 | 4.65 | 15.35 | 20 | #N/A | pSBX1K3-SspDnaXc*-sfGFP^C-H6 - O275-4 | #N/A | ||||||||||||||||||
563 | DG7167 | O275-5 | pSBX1K3-SspDnaXc*-sfGFP^C-H6 | 40 | 4.65 | 15.35 | 20 | #N/A | pSBX1K3-SspDnaXc*-sfGFP^C-H6 - O275-5 | #N/A | ||||||||||||||||||
564 | DG7168 | O312-4 | pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 | 40 | 3.40 | 16.60 | 20 | #N/A | pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225 - O312-4 | #N/A | ||||||||||||||||||
565 | DG7169 | O313-4 | pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 | 40 | 2.75 | 17.25 | 20 | #N/A | ok, good | pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168 - O313-4 | #N/A | |||||||||||||||||
566 | DG7170 | O314-4 | pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 | 40 | 2.62 | 17.38 | 20 | #N/A | pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 - O314-4 | #N/A | ||||||||||||||||||
567 | DG7171 | O314-5 | pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 | 40 | 3.20 | 16.80 | 20 | #N/A | pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225 - O314-5 | #N/A | ||||||||||||||||||
568 | DG7172 | O315-4 | pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 | 40 | 4.37 | 15.63 | 20 | td> | #N/A | ok, good | pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 - O315-4 | #N/A | ||||||||||||||||
569 | DG7173 | O315-5 | pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 | 40 | 2.99 | 17.01 | 20 | #N/A | ok, but probably mutation in promoter, if possible use O315-4 | pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168 - O315-5 | #N/A | |||||||||||||||||
570 | DG7142 | O414 | pSB1C3-1GateSspDnaB(C*) | 40 | 3.32 | 16.68 | 20 | VF2 | bad | pSB1C3-1GateSspDnaB(C*) - O414 | tgccacctgacgtctaagaa | |||||||||||||||||
571 | DG7143 | O414-2 | pSB1C3-1GateSspDnaB(C*) | 40 | 3.54 | 16.46 | 20 | VF2 | pSB1C3-1GateSspDnaB(C*) - O414-2 | tgccacctgacgtctaagaa | ||||||||||||||||||
572 | DG7144 | O414-3 | pSB1C3-1GateSspDnaB(C*) | 40 | 4.97 | 15.03 | 20 | VF2 | pSB1C3-1GateSspDnaB(C*) - O414-3 | tgccacctgacgtctaagaa | ||||||||||||||||||
573 | DG7145 | O414-4 | pSB1C3-1GateSspDnaB(C*) | 40 | 4.15 | 15.85 | 20 | VF2 | pSB1C3-1GateSspDnaB(C*) - O414-4 | tgccacctgacgtctaagaa | ||||||||||||||||||
574 | DG7146 | O415 | pSB1C3-1GateSspDnaB(N) | 40 | 5.56 | 14.44 | 20 | VR | bad | pSB1C3-1GateSspDnaB(N) - O415 | attaccgcctttgagtgagc | |||||||||||||||||
575 | DG7147 | O415-2 | pSB1C3-1GateSspDnaB(N) | 40 | 7.21 | 12.79 | 20 | VR | bad | pSB1C3-1GateSspDnaB(N) - O415-2 | attaccgcctttgagtgagc | |||||||||||||||||
576 | DG7148 | O415-3 | pSB1C3-1GateSspDnaB(N) | 40 | 2.54 | 17.46 | 20 | VR | bad | pSB1C3-1GateSspDnaB(N) - O415-3 | attaccgcctttgagtgagc | |||||||||||||||||
577 | DG7149 | O415-4 | pSB1C3-1GateSspDnaB(N) | 40 | 3.76 | 16.24 | 20 | VR | bad | pSB1C3-1GateSspDnaB(N) - O415-4 | attaccgcctttgagtgagc | |||||||||||||||||
578 | DG7150 td> | O416 | pSB1C3-1GateSspDnaB(N*) | 40 | 4.32 | 15.68 | 20 | VR | bad | pSB1C3-1GateSspDnaB(N*) - O416 | attaccgcctttgagtgagc | |||||||||||||||||
579 | DG7151 | O416-2 | pSB1C3-1GateSspDnaB(N*) | 40 | 10.96 | 9.04 | 20 | VR | not mixed up, missing? | pSB1C3-1GateSspDnaB(N*) - O416-2 | attaccgcctttgagtgagc | |||||||||||||||||
580 | DG7152 | O411 | pSB1C3-1GateSspDnaE(N) | 40 | 5.56 | 14.44 | 20 | VR | pSB1C3-1GateSspDnaE(N) - O411 | attaccgcctttgagtgagc | ||||||||||||||||||
581 | DG7153 | O411-2 | pSB1C3-1GateSspDnaE(N) | 40 | 4.32 | 15.68 | 20 | VR | pSB1C3-1GateSspDnaE(N) - O411-2 | attaccgcctttgagtgagc | ||||||||||||||||||
582 | DG7154 | O411-3 | pSB1C3-1GateSspDnaE(N) | 40 | 3.54 | 16.46 | 20 | VR | pSB1C3-1GateSspDnaE(N) - O411-3 | attaccgcctttgagtgagc | ||||||||||||||||||
583 | DG7155 | O411-4 | pSB1C3-1GateSspDnaE(N) | 40 | 4.82 | 15.18 | 20 | VR | pSB1C3-1GateSspDnaE(N) - O411-4 | attaccgcctttgagtgagc | ||||||||||||||||||
584 | DG7156 | O412 | pSB1C3-1GateSspDnaE(C) | 40 | 7.08 | 12.92 | 20 | VF2 | pSB1C3-1GateSspDnaE(C) - O412 | |||||||||||||||||||
585 | DG7157 | O412-2 | pSB1C3-1GateSspDnaE(C) | 40 | 2.37 | 17.63 | 20 | VF2 | pSB1C3-1GateSspDnaE(C) - O412-2 | |||||||||||||||||||
586 | DG7158 | O412-3 | pSB1C3-1GateSspDnaE(C) | 40 | 10.00 | 10.00 | 20 | VF2 | pSB1C3-1GateSspDnaE(C) - O412-3 | |||||||||||||||||||
587 | DG7159 | O412-4 | pSB1C3-1GateSspDnaE(C) | 40 | 5.76 | 14.24 | 20 | VF2 | pSB1C3-1GateSspDnaE(C) - O412-4 | |||||||||||||||||||
588 | DG7160 | O413 | pSB1C3-1GateSspDnaB(C) | 40 | 8.89 td> | 11.11 | 20 | VF2 | pSB1C3-1GateSspDnaB(C) - O413 | |||||||||||||||||||
589 | DG7161 | O413-2 | pSB1C3-1GateSspDnaB(C) | 40 | 13.79 | 6.21 | 20 | VF2 | bad | pSB1C3-1GateSspDnaB(C) - O413-2 | ||||||||||||||||||
590 | IC5213 | 10-07 | O415 | pSB1C3-1GateSspDnaB(N) | 40 | 5.56 | 14.44 | 20 | "020291" | VF2 | actually SspDnaEC | pSB1C3-1GateSspDnaB(N) - O415 | ||||||||||||||||
591 | IC5214 | 10-07 | O413-2 | pSB1C3-1GateSspDnaB(C) | 40 | 13.79 | 6.21 | 20 | "020291" | VF2 | actually SspDnaBN* | pSB1C3-1GateSspDnaB(C) - O413-2 | ||||||||||||||||
592 | IC5215 | 10-07 | O416 | pSB1C3-1GateSspDnaB(N*) | 40 | 4.32 | 15.68 | 20 | "020291" | VF2 | actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie | pSB1C3-1GateSspDnaB(N*) - O416 | ||||||||||||||||
593 | IC5216 | 10-07 | O414 | pSB1C3-1GateSspDnaB(C*) | 40 | 3.32 | 16.68 | 20 | "020291" | VF2 | SspDnaEN td> | pSB1C3-1GateSspDnaB(C*) - O414 | ||||||||||||||||
594 | IC5217 | 10-07 | O411-4 | pSB1C3-1GateSspDnaE(N) | 40 | 4.82 | 15.18 | 20 | "020291" | VF2 | actually SspDnaBC* | pSB1C3-1GateSspDnaE(N) - O411-4 | ||||||||||||||||
595 | IC5218 | 10-07 | O415 | pSB1C3-1GateSspDnaB(N) | 40 | 5.56 | 14.44 | 20 | "020292" | VR | actually SspDnaEC | pSB1C3-1GateSspDnaB(N) - O415 | ||||||||||||||||
596 | IC5219 | 10-07 | O413-2 | pSB1C3-1GateSspDnaB(C) | 40 | 13.79 | 6.21 | 20 | "020292" | VR | actually SspDnaBN* | pSB1C3-1GateSspDnaB(C) - O413-2 | ||||||||||||||||
597 | IC5220 | 10-07 | O416 | pSB1C3-1GateSspDnaB(N*) | 40 | 4.32 | 15.68 | 20 | "020292" | VR | actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie | pSB1C3-1GateSspDnaB(N*) - O416 | ||||||||||||||||
598 | IC5221 | 10-07 | O414 | pSB1C3-1GateSspDnaB(C*) | 40 | 3.32 | 16.68 | 20 | "020292" | VR | SspDnaEN | pSB1C3-1GateSspDnaB(C*) - O414 td> | ||||||||||||||||
599 | IC5222 | 10-07 | O411-4 | pSB1C3-1GateSspDnaE(N) | 40 | 4.82 | 15.18 | 20 | "020292" | VR | actually SspDnaBC* | pSB1C3-1GateSspDnaE(N) - O411-4 | ||||||||||||||||
600 | IC5223 | 10-07 | O403-3 | pSB1C3-T7RBS_BBv2 | 40 | 2.32 | 17.68 | 20 | "020292" | VR | pSB1C3-T7RBS_BBv2 - O403-3 | |||||||||||||||||
601 | IC5224 | 10-07 | O403-4 | pSB1C3-T7RBS_BBv2 | 40 | 2.80 | 17.20 | 20 | "020292" | VR | pSB1C3-T7RBS_BBv2 - O403-4 | |||||||||||||||||
602 | IC5185 | 10-07 | O386 | pSBX4C5-0GateTer*-coex_mRFP168/169 | 40 | 2.67 | 17.33 | 20 | "020293" | 1 | good, ok | pSBX4C5-0GateTer*-coex_mRFP168/169 - O386 | ||||||||||||||||
603 | IC5186 | 10-07 | O386 | pSBX4C5-0GateTer*-coex_mRFP168/169 | 40 | 2.67 | 17.33 | 20 | "020295" | 2 | good, ok | pSBX4C5-0GateTer*-coex_mRFP168/169 - O386 | ||||||||||||||||
604 | IC5187 | 10-07 | O387 | pSBX4C5-0GateNpu*-coex_mRFP168/169 | 40 | 2.50 | 17.50 | 20 | "020293" | 1 | good, ok | pSBX4C5-0GateNpu*-coex_mRFP168/169 - O387 | ||||||||||||||||
605 | IC5188 | 10-07 | O387 | pSBX4C5-0GateNpu*-coex_mRFP168/169 | 40 | 2.50 | 17.50 | 20 | "020295" | 2 | good, ok | pSBX4C5-0GateNpu*-coex_mRFP168/169 - O387 | ||||||||||||||||
606 | IC5189 | 10-07 | O384 | pSBX4C5-0GateDnaX*-coex_mRFP168/169 | 40 | 1.45 | 18.55 | 20 | "020293" | 1 | no DnaXN | pSBX4C5-0GateDnaX*-coex_mRFP168/169 - O384 | ||||||||||||||||
607 | IC5190 | 10-07 | O384 | pSBX4C5-0GateDnaX*-coex_mRFP168/169 | 40 | 1.45 | 18.55 | 20 | "020295" | 2 | no DNnaXN | pSBX4C5-0GateDnaX*-coex_mRFP168/169 - O384 | ||||||||||||||||
608 | IC5191 | 10-07 | O397 | pSBX1K3-DnaX-mRFP168/169_coex | 40 | 3.48 | 16.52 | 20 | "020293" | 1 | good, ok | pSBX1K3-DnaX-mRFP168/169_coex - O397 | ||||||||||||||||
609 | IC5192 | 10-07 | O397 | pSBX1K3-DnaX-mRFP168/169_coex | 40 | 3.48 | 16.52 | 20 | "020295" | 2 | good, ok | pSBX1K3-DnaX-mRFP168/169_coex - O397 | ||||||||||||||||
610 | IC5193 | 10-07 | O255-3 | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 | 40 | 2.35 | 17.65 | 20 | "020293" | 1 | good, ok | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 - O255-3 | ||||||||||||||||
611 | IC5194 | 10-07 | O255-3 | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 | 40 | 2.35 | 17.65 | 20 | "020295" | 2 | good, ok | pSBX4C5-0GateSspDnaX-coex_mRFP168/169 - O255-3 | ||||||||||||||||
612 | IC5195 | 10-07 | O256 | pSBX4C5-0GateSspGyrB-coex_mRFP168/169 | 40 | 2.22 | 17.78 | 20 | "020293" | 1 | good, ok | pSBX4C5-0GateSspGyrB-coex_mRFP168/169 - O256 | ||||||||||||||||
613 | IC5196 | 10-07 | O256 | pSBX4C5-0GateSspGyrB-coex_mRFP168/169 | 40 | 2.22 | 17.78 | 20 | "020295" | 2 | good, ok | pSBX4C5-0GateSspGyrB-coex_mRFP168/169 - O256 | ||||||||||||||||
614 | IC5197 | 10-07 | O257-3 | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 | 40 | 1.45 | 18.55 | 20 | "020293" | 1 | mutations after Biobrick, good ok | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 - O257-3 | ||||||||||||||||
615 | IC5198 | 10-07 | O257-3 | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 | 40 | 1.45 | 18.55 | 20 | "020295" | 2 | mutations after Biobrick, good ok | pSBX4C5-0GateTerDnaE3-coex_mRFP168/169 - O257-3 | ||||||||||||||||
616 | IC5199 | 10-07 | O393 | pSBX1K3-H6-LOV-BsaIIasB-mRFP_coex | 40 | 3.08 | 16.92 | 20 | "020293" | 1 | ok, good | pSBX1K3-H6-LOV-BsaIIasB-mRFP_coex - O393 | ||||||||||||||||
617 | IC5200 | 10-07 | O393 | pSBX1K3-H6-LOV-BsaIIasB-mRFP_coex | 40 | 3.08 | 16.92 | 20 | "020295" | 2 | ok, good | pSBX1K3-H6-LOV-BsaIIasB-mRFP_coex - O393 | ||||||||||||||||
618 | IC5201 | 10-07 | O396 | pSBX1K3-DnaX-mRFP*168/169_coex | 40 | 3.20 | 16.80 | 20 | "020294" | 1 | ok, good | pSBX1K3-DnaX-mRFP*168/169_coex - O396 | ||||||||||||||||
619 | IC5202 | 10-07 | O396 | pSBX1K3-DnaX-mRFP*168/169_coex | 40 | 3.20 | 16.80 | 20 | "020296" | 2 | ok, good | pSBX1K3-DnaX-mRFP*168/169_coex - O396 | ||||||||||||||||
620 | IC5203 | 10-07 | O395 | pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex | 40 | 2.67 | 17.33 | 20 | "020294" | 1 | ok, good, probably frameshift after CDS | pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex - O395 | ||||||||||||||||
621 | IC5204 | 10-07 | O395 | pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex | 40 | 2.67 | 17.33 | 20 | "020296" | 2 | ok, good, probably frameshift after CDS | pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex - O395 | ||||||||||||||||
622 | IC5205 | 10-07 | O391 | pSBX1K3-DnaX-sfGFP_coex | 40 | 2.42 | 17.58 | 20 | "020294" | 1 | ok, good | pSBX1K3-DnaX-sfGFP_coex - O391 | ||||||||||||||||
623 | IC5206 | 10-07 | O391 | pSBX1K3-DnaX-sfGFP_coex | 40 | 2.42 | 17.58 | 20 | "020296" | 2 | ok,good | pSBX1K3-DnaX-sfGFP_coex - O391 | ||||||||||||||||
624 | td> | IC5207 | 10-07 | O392 | pSBX1K3-DnaX*-sfGFP_coex | 40 | 3.81 | 16.19 | 20 | "020294" | 1 | ok, good | pSBX1K3-DnaX*-sfGFP_coex - O392 | |||||||||||||||
625 | IC5208 | 10-07 | O392 | pSBX1K3-DnaX*-sfGFP_coex | 40 | 3.81 | 16.19 | 20 | "020296" | 2 | ok,good | pSBX1K3-DnaX*-sfGFP_coex - O392 | ||||||||||||||||
626 | IC5209 | 10-07 | O388 | pSBX1K3-LOV-BsaIIasB-sfGFP_coex | 40 | 4.21 | 15.79 | 20 | "020294" | 1 | ok, good | pSBX1K3-LOV-BsaIIasB-sfGFP_coex - O388 | ||||||||||||||||
627 | IC5210 | 10-07 | O388 | pSBX1K3-LOV-BsaIIasB-sfGFP_coex | 40 | 4.21 | 15.79 | 20 | "020296" | 2 | ok,good | pSBX1K3-LOV-BsaIIasB-sfGFP_coex - O388 | ||||||||||||||||
628 | IC5211 | 10-07 | O389 | pSBX1K3-LOV-12-DnaXC-sfGFP_coex | 40 | 2.96 | 17.04 | 20 | "020294" | 1 | ok, good | pSBX1K3-LOV-12-DnaXC-sfGFP_coex - O389 | ||||||||||||||||
629 | IC5212 | 10- 07 | O389 | pSBX1K3-LOV-12-DnaXC-sfGFP_coex | 40 | 2.96 | 17.04 | 20 | "020296" | 2 | ok,good | pSBX1K3-LOV-12-DnaXC-sfGFP_coex - O389 | ||||||||||||||||
630 | IC5225 | 10-07 | O235 | pSB1C3-Smt3-2ZSortA-H6 (mRFP) | 166.47 | 20.00 | 0.00 | 20 | BAC1 | C->A point mutation in mRFP barcode | pSB1C3-Smt3-2ZSortA-H6 (mRFP) - O235 | |||||||||||||||||
631 | IC5226 | 10-07 | O235-2 | pSB1C3-Smt3-2ZSortA-H6 (mRFP) | 197.63 | 20.00 | 0.00 | 20 | BAC1 | C->A point mutation in mRFP barcode | pSB1C3-Smt3-2ZSortA-H6 (mRFP) - O235-2 | |||||||||||||||||
632 | IC5227 | 10-07 | O177-4 | pSB1C3-2ZSortA-H6 | 286.76 | 20.00 | 0.00 | 20 | BAC1 | ok | pSB1C3-2ZSortA-H6 - O177-4 | |||||||||||||||||
633 | IC5228 | 10-07 | O177-5 | pSB1C3-2ZSortA-H6 | 299.4 | 20.00 | 0.00 | 20 | BAC1 | ok | pSB1C3-2ZSortA-H6 - O177-5 | |||||||||||||||||
634 | IC5229 | 10-07 | O426 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 278.7 | 20.00 | 0.00 | 20 | "020294" | 1 | is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP | pSB1C3-H6-Smt3-mDNMT1(731-1602) - O426 | ||||||||||||||||
635 | IC5230 | 10-07 | O426-2 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 322.85 | 20.00 | 0.00 | 20 | "020291" | #N/A | ok | pSB1C3-H6-Smt3-mDNMT1(731-1602) - O426-2 | ||||||||||||||||
636 | IC5231 | 10-07 | O235 | pSB1C3-Smt3-2ZSortA-H6 (mRFP) | 20 | "020291" | #N/A | mixture of mRFP and ccdB | pSB1C3-Smt3-2ZSortA-H6 (mRFP) - O235 | |||||||||||||||||||
637 | O235-2 | pSB1C3-Smt3-2ZSortA-H6 (mRFP) | 20 | #N/A | pSB1C3-Smt3-2ZSortA-H6 (mRFP) - O235-2 | |||||||||||||||||||||||
638 | IC5232 | 10-07 | O177-4 | pSB1C3-2ZSortA-H6 | 20 | "020294" | 1 | ok | pSB1C3-2ZSortA-H6 - O177-4 | |||||||||||||||||||
639 | IC5233 | 10-07 | O177-5 | pSB1C3-2ZSortA-H6 | 20 | "020291" | #N/A | ok | td> | pSB1C3-2ZSortA-H6 - O177-5 | ||||||||||||||||||
640 | IC5234 | 10-07 | O426 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 20 | "020296" | 2 | is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP | pSB1C3-H6-Smt3-mDNMT1(731-1602) - O426 | |||||||||||||||||||
641 | IC5235 | 10-07 | O426-2 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 20 | "020296" | 2 | ok | pSB1C3-H6-Smt3-mDNMT1(731-1602) - O426-2 | |||||||||||||||||||
642 | IC5236 | 10-07 | O426 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 20 | "020297" | 26 | no result | pSB1C3-H6-Smt3-mDNMT1(731-1602) - O426 | |||||||||||||||||||
643 | DD1481 | 10-07 | O426-2 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 20 | "020297" | 26 | ok | pSB1C3-H6-Smt3-mDNMT1(731-1602) - O426-2 | |||||||||||||||||||
644 | DD1482 | 10-07 | O426 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 20 | "020298" | 27 | is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP | pSB1C3-H6-Smt3-mDNMT1(731-1602) - O426 | td> | ||||||||||||||||||
645 | DD1483 | 10-07 | O426-2 | pSB1C3-H6-Smt3-mDNMT1(731-1602) | 20 | "020298" | 27 | ok | pSB1C3-H6-Smt3-mDNMT1(731-1602) - O426-2 | |||||||||||||||||||
646 | "020293" | 10-07 | 1 | VF2 | 20.00 | 0.00 | 20 | #N/A | VF2 - 1 | |||||||||||||||||||
647 | "020294" | 10-07 | 1 | VF2 | 20.00 | 0.00 | 20 | #N/A | VF2 - 1 | |||||||||||||||||||
648 | "020295" | 10-07 | 2 | VR | 20.00 | 0.00 | 20 | #N/A | VR - 2 | |||||||||||||||||||
649 | "020296" | 10-07 | 2 | VR | 20.00 | 0.00 | 20 | VR - 2 | ||||||||||||||||||||
650 | "020297" | 10-07 | 26 | DNMT1_P2_R_deg.seq.731-1602 | 20.00 | 0.00 | 20 | #N/A | DNMT1_P2_R_deg.seq.731-1602 - 26 | |||||||||||||||||||
651 | "020298" | 10-07 | 27 | DNMT1_P3_F_both_seq.731-1602 | 20.00 | 0.00 | 20 | #N/A | DNMT1_P3_F_both_seq.731-1602 - 27 | |||||||||||||||||||
652 | IC5260 | 10-08 | O412-2 | pSB1C3-1GateSspDnaE(C) | 337 | 20.00 | 0.00 | 20 | VR | actually sspdnaBN | pSB1C3-1GateSspDnaE(C) - O412-2 | |||||||||||||||||
653 | IC5261 | 10-08 | O412-3 | pSB1C3-1GateSspDnaE(C) | 337 | 20.00 | 0.00 | 20 | VF2 | actually sspdnaBN | pSB1C3-1GateSspDnaE(C) - O412-3 | |||||||||||||||||
654 | DD2689 | 10-08 | O394 | pSBX1K3-H6-LOV-12-DnaXC-mRFP_coex | 40 | #DIV/0! | #DIV/0! | 20 | "937419" | VF2 | ok, good | pSBX1K3-H6-LOV-12-DnaXC-mRFP_coex - O394 | ||||||||||||||||
655 | DD2688 | 10-08 | O394 | pSBX1K3-H6-LOV-12-DnaXC-mRFP_coex | 40 | #DIV/0! | #DIV/0! | 20 | "937420" | VR | ok, good | pSBX1K3-H6-LOV-12-DnaXC-mRFP_coex - O394 | ||||||||||||||||
656 | DD2690 | 10-10 | O260-6 | pSBX1K3-BBa_I13507-GSGIDECHT | 40 | 6.64 | 13.36 | 20 | 937421 | VR | pSBX1K3-BBa_I13507-GSGIDECHT - O260-6 | |||||||||||||||||
657 | DD2691 | 10-10 | O260-6 | pSBX1K3-BBa_I13507-GSGIDECHT | 40 | 6.64 | 13.36 | 20 | 937422 | VF2 | pSBX1K3-BBa_I13507-GSGIDECHT - O260-6 | |||||||||||||||||
658 | DD2692 | 10-10 | O260-7 | pSBX1K3-BBa_I13507-GSGIDECHT | 40 | 5.52 | 14.48 | 20 | 937421 | VR | pSBX1K3-BBa_I13507-GSGIDECHT - O260-7 | |||||||||||||||||
659 | DD2693 | 10-10 | O260-7 | pSBX1K3-BBa_I13507-GSGIDECHT | 40 | 5.52 | 14.48 | 20 | 937422 | VF2 | pSBX1K3-BBa_I13507-GSGIDECHT - O260-7 | |||||||||||||||||
660 | DD2694 | 10-10 | O261-4 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 40 | 5.84 | 14. 16 | 20 | 937421 | VR | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha - O261-4 | |||||||||||||||||
661 | DD2695 | 10-10 | O261-4 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 40 | 5.84 | 14.16 | 20 | 937422 | VF2 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha - O261-4 | |||||||||||||||||
662 | DD2696 | 10-10 | O261-5 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 40 | 7.48 | 12.52 | 20 | 937421 | VR | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha - O261-5 | |||||||||||||||||
663 | DD2697 | 10-10 | O261-5 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 40 | 7.48 | 12.52 | 20 | 937422 | VF2 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha - O261-5 | |||||||||||||||||
664 | DD2698 | 10-10 | O261-6 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 40 | 7.48 | 12.52 | 20 | 937421 | VR | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha - O261-6 | |||||||||||||||||
665 | DD2699 | 10-10 | O261-6 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha | 40 | 7.48 | 12.52 | 20 | 937422 | VF2 | pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha - O261-6 | td> | ||||||||||||||||
666 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
667 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
668 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
669 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
670 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
671 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
672 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
673 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
674 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
675 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
676 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
677 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
678 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
679 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
680 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
681 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
682 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
683 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
684 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
685 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
686 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
687 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
688 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
689 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
690 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
691 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
692 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
693 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
694 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
695 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
696 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
697 | td> | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | ||||||||||||||||||||
698 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A | |||||||||||||||||||||
699 | #N/A | #N/A | #N/A | #N/A | 20 | #N/A | #N/A |