Team:Heidelberg/AwesomeSheet/ComCells

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– Comptent Cells

ACDEFGHIJKLMNOPQRSTUV
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Batch ID
DateWhoGrowthOrganismFinal ResuspensionAliquotsAre emptyPlasmids
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IDVVStorage BufferV
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mlIDSpeciesStrainMethodmlµLEfficiencyComment9/9/20149/13/20149/19/20149/28/201410/3/2014
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16/1/2014Florian Schmidt3E. coliTurbosPEG MgCl280yes7.00E+05
5
206-05Silvan, Jan1C1-31E. coliTOP10CaCl2
10% Glycerol 90 mM CaCl2 in H20
1001.00E+065.00E+055.20E+04<2*e3
6
306-21Silvan, Jan and Jakob11C1-15E. coliOne ShotCaCl2
10% Glycerol 90 mM CaCl2 in H20
50yes6.20E+05
7
406-26Charlotte Bunne, Anna, Jan66C1-27E. coli
Rosetta (DE3)
CaCl2
10% Glycerol 90 mM CaCl2 in H20
501.50E+053.10E+01
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506-26Charlotte Bunne, Anna, Jan66C1-27E. coli
Rosetta (DE3)
CaCl2
10% Glycerol 90 mM CaCl2 in H20
50yes5.00E+043.10E+01
douBIe concentration (only 0.5 volumes final resupension volume)
HC
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67/6/2014Jan134C1-27E. coli
Rosetta (DE3)
CaCl2
100 mM CaCl2 in H20
300yes31,30
in situ competent cells in order to get N and C inteins both into the cells
10
77/6/2014Jan137C1-27E. coli
Rosetta (DE3)
CaCl2
100 mM CaCl2 in H20
300yes31,29
in situ competent cells in order to get N and C inteins both into the cells
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87/12/2014Silvan12C16E. coliBI21(DE3)CaCl2
10% Glycerol 90 mM CaCl2 in H20
yesin situ
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97/20/2014Silvan12C2-16E. coliBI21(DE3)CaCl2
10% Glycerol 90 mM CaCl2 in H20
50yes1.80E+04< 2000
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107/28/2014Charlotte12C3-12006E. coliBI21(DE3)CaCl25
10% Glycerol 90 mM CaCl2 in H20
100yes8.00E+05RFC99,w/o liquid nitrogen and cold room
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1107-31Jan6C1-22506E. coliBI21(DE3)CaCl21
15% Glycerol 100 mM CaCl2 in H20
50yes1.50E+03
15
1207-31Jan6C1-32506E. coliBI21(DE3)CaCl21
15% Glycerol 100 mM CaCl2 in H20
50yes2.00E+03
16
137/30/2014Sabine1E. coliTOP1050yes5.00E+06w/o liquid nitrogen and cold room in special eppis
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1408-02Charlotte3C1-21003E. coliTurbosCaCl2
15% Glycerol 100 mM CaCl2 in H20
100yes
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1508-12Charlotte3C1-3503E. coliTurbosCaCl2
15% Glycerol 100 mM CaCl2 in H20
50yes1.38E+07
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168/30/2014Charlotte3C1-52003E. coliTurbosCaCl2
15% Glycerol 100 mM CaCl2 in H20
80yes3.40E+06
20
179/8/2014Charlotte, Silvan6C1-112006E. coliBI21(DE3)CaCl25
15% Glycerol 100 mM CaCl2 in H20
801.65E+04RFC99,w/o liquid nitrogen and cold room1.00E+04
21
189/8/2014Charlotte3C1-62003E. coliTurbosCaCl25
15% Glycerol 100 mM CaCl2 in H20
80yes3.50E+06RFC99,w/o liquid nitrogen and cold room8.00E+05
22
199/9/2014Charlotte, Jan6C1-132006E. coliBI21(DE3)CaCl25
10% Glycerol 90 mM CaCl2 in H20
1006.00E+04RFC99,w/o liquid nitrogen and cold room6.00E+04
23
209/15/2014Charlotte3C1-72003E. coliTurbosCaCl25
10% Glycerol 90 mM CaCl2 in H20
80yes8.00E+058.00E+05
24
219/25/2014Florian Schmidt1E. coliTOP10Inoueyes
25
229/27/2014Jan3C1-91003E. coliTurbosCaCl23
10% Glycerol 90 mM CaCl2 in H20
1001.40E+06RFC99,w/o liquid nitrogen and cold room1.40E+066.05E+04
26
239/27/2014Jan3C1-102003E. coliTurbosCaCl25
10% Glycerol 90 mM CaCl2 in H20
100yes1.60E+06RFC99,w/o liquid nitrogen and cold room1.60E+06
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249/28/2014Charlotte6C1-182006E. coliBI21(DE3)CaCl2
10% Glycerol 90 mM CaCl2 in H21
80
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2510/1/2014Jan3C1-132003E. coliTurbosInoue like20
1%(v/v) DMSO in TB (Inoue II)
100
cells were grown at 37°C not 18°C, some aliquots are 50 and some 80
2.36E+05
29
2610/1/2014Jan3C1-132003E. coliTurbosCaCl25
10% Glycerol 90 mM CaCl2 in H21
100
first cetrifugation was just 10 min, both centrifugations were at just 2500g
1.39E+05
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27#N/A#N/A
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28#N/A#N/A
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29#N/A#N/A
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30#N/A#N/A
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31#N/A#N/A
35
32#N/A#N/A
36
33#N/A#N/A
37
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35#N/A#N/A
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36#N/A#N/A
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37#N/A#N/A