Team:Heidelberg/AwesomeSheet/ComCells

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– Comptent Cells

	A	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V
1	Batch ID	Date	Who	Growth		Organism				Final Resuspension		Aliquots	Are empty		Plasmids
2				ID	V					V	Storage Buffer	V
3					ml	ID	Species	Strain	Method	ml		µL		Efficiency		Comment	9/9/2014	9/13/2014	9/19/2014	9/28/2014	10/3/2014

4	1	6/1/2014	Florian Schmidt			3	E. coli	Turbos	PEG MgCl2			80	yes	7.00E+05
5	2	06-05	Silvan, Jan	1C1-3		1	E. coli	TOP10	CaCl2		10% Glycerol 90 mM CaCl2 in H20	100		1.00E+06			5.00E+05		5.20E+04	<2*e3
6	3	06-21	Silvan, Jan and Jakob	11C1-1		5	E. coli	One Shot	CaCl2		10% Glycerol 90 mM CaCl2 in H20	50	yes	6.20E+05
7	4	06-26	Charlotte Bunne, Anna, Jan	66C1-2		7	E. coli	Rosetta (DE3)	CaCl2		10% Glycerol 90 mM CaCl2 in H20	50		1.50E+05	3.10E+01
8	5	06-26	Charlotte Bunne, Anna, Jan	66C1-2		7	E. coli	Rosetta (DE3)	CaCl2		10% Glycerol 90 mM CaCl2 in H20	50	yes	5.00E+04	3.10E+01	douBIe concentration (only 0.5 volumes final resupension volume)	HC
9	6	7/6/2014	Jan	134C1-2		7	E. coli	Rosetta (DE3)	CaCl2		100 mM CaCl2 in H20	300	yes		31,30	in situ competent cells in order to get N and C inteins both into the cells
10	7	7/6/2014	Jan	137C1-2		7	E. coli	Rosetta (DE3)	CaCl2		100 mM CaCl2 in H20	300	yes		31,29	in situ competent cells in order to get N and C inteins both into the cells
11	8	7/12/2014	Silvan	12C1		6	E. coli	BI21(DE3)	CaCl2		10% Glycerol 90 mM CaCl2 in H20		yes			in situ
12	9	7/20/2014	Silvan	12C2-1		6	E. coli	BI21(DE3)	CaCl2		10% Glycerol 90 mM CaCl2 in H20	50	yes	1.80E+04				< 2000
13	10	7/28/2014	Charlotte	12C3-1	200	6	E. coli	BI21(DE3)	CaCl2	5	10% Glycerol 90 mM CaCl2 in H20	100	yes	8.00E+05		RFC99,w/o liquid nitrogen and cold room
14	11	07-31	Jan	6C1-2	250	6	E. coli	BI21(DE3)	CaCl2	1	15% Glycerol 100 mM CaCl2 in H20	50	yes	1.50E+03
15	12	07-31	Jan	6C1-3	250	6	E. coli	BI21(DE3)	CaCl2	1	15% Glycerol 100 mM CaCl2 in H20	50	yes	2.00E+03
16	13	7/30/2014	Sabine			1	E. coli	TOP10				50	yes	5.00E+06		w/o liquid nitrogen and cold room in special eppis
17	14	08-02	Charlotte	3C1-2	100	3	E. coli	Turbos	CaCl2		15% Glycerol 100 mM CaCl2 in H20	100	yes
18	15	08-12	Charlotte	3C1-3	50	3	E. coli	Turbos	CaCl2		15% Glycerol 100 mM CaCl2 in H20	50	yes	1.38E+07
19	16	8/30/2014	Charlotte	3C1-5	200	3	E. coli	Turbos	CaCl2		15% Glycerol 100 mM CaCl2 in H20	80	yes	3.40E+06
20	17	9/8/2014	Charlotte, Silvan	6C1-11	200	6	E. coli	BI21(DE3)	CaCl2	5	15% Glycerol 100 mM CaCl2 in H20	80		1.65E+04		RFC99,w/o liquid nitrogen and cold room		1.00E+04
21	18	9/8/2014	Charlotte	3C1-6	200	3	E. coli	Turbos	CaCl2	5	15% Glycerol 100 mM CaCl2 in H20	80	yes	3.50E+06		RFC99,w/o liquid nitrogen and cold room		8.00E+05
22	19	9/9/2014	Charlotte, Jan	6C1-13	200	6	E. coli	BI21(DE3)	CaCl2	5	10% Glycerol 90 mM CaCl2 in H20	100		6.00E+04		RFC99,w/o liquid nitrogen and cold room		6.00E+04
23	20	9/15/2014	Charlotte	3C1-7	200	3	E. coli	Turbos	CaCl2	5	10% Glycerol 90 mM CaCl2 in H20	80	yes	8.00E+05					8.00E+05
24	21	9/25/2014	Florian Schmidt			1	E. coli	TOP10	Inoue				yes
25	22	9/27/2014	Jan	3C1-9	100	3	E. coli	Turbos	CaCl2	3	10% Glycerol 90 mM CaCl2 in H20	100		1.40E+06		RFC99,w/o liquid nitrogen and cold room				1.40E+06	6.05E+04
26	23	9/27/2014	Jan	3C1-10	200	3	E. coli	Turbos	CaCl2	5	10% Glycerol 90 mM CaCl2 in H20	100	yes	1.60E+06		RFC99,w/o liquid nitrogen and cold room				1.60E+06
27	24	9/28/2014	Charlotte	6C1-18	200	6	E. coli	BI21(DE3)	CaCl2		10% Glycerol 90 mM CaCl2 in H21	80
28	25	10/1/2014	Jan	3C1-13	200	3	E. coli	Turbos	Inoue like	20	1%(v/v) DMSO in TB (Inoue II)	100				cells were grown at 37°C not 18°C, some aliquots are 50 and some 80					2.36E+05
29	26	10/1/2014	Jan	3C1-13	200	3	E. coli	Turbos	CaCl2	5	10% Glycerol 90 mM CaCl2 in H21	100				first cetrifugation was just 10 min, both centrifugations were at just 2500g					1.39E+05
30	27					#N/A		#N/A
31	28					#N/A		#N/A
32	29					#N/A		#N/A
33	30					#N/A		#N/A
34	31					#N/A		#N/A
35	32					#N/A		#N/A
36	33					#N/A		#N/A
37	34					#N/A		#N/A
38	35					#N/A		#N/A
39	36					#N/A		#N/A
40	37					#N/A		#N/A

Team:Heidelberg/AwesomeSheet/ComCells

From 2014.igem.org

– Comptent Cells

Awesome Sheet PA Gels Dnmt1 Methylation Assay Blots Waste SURPREMErun Strains Stocks Samples Purifications Plate Reader Marker LIGHTRun Enzymes Competent cells Induction Growth 3AL AGels AssmProd Assembly GoldenGate Parts Submission Parts PCR products PCRs Plasmids Plates Primer Res SL Trafos