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Team:Heidelberg/AwesomeSheet/ComCells
From 2014.igem.org
Lab book
– Comptent Cells
| A | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Batch ID | Date | Who | Growth | Organism | Final Resuspension | Aliquots | Are empty | Plasmids | |||||||||||||
2 | ID | V | V | Storage Buffer | V | |||||||||||||||||
3 | ml | ID | Species | Strain | Method | ml | µL | Efficiency | Comment | 9/9/2014 | 9/13/2014 | 9/19/2014 | 9/28/2014 | 10/3/2014 | ||||||||
4 | 1 | 6/1/2014 | Florian Schmidt | 3 | E. coli | Turbos | PEG MgCl2 | 80 | yes | 7.00E+05 | ||||||||||||
5 | 2 | 06-05 | Silvan, Jan | 1C1-3 | 1 | E. coli | TOP10 | CaCl2 | 10% Glycerol 90 mM CaCl2 in H20 | 100 | 1.00E+06 | 5.00E+05 | 5.20E+04 | <2*e3 | ||||||||
6 | 3 | 06-21 | Silvan, Jan and Jakob | 11C1-1 | 5 | E. coli | One Shot | CaCl2 | 10% Glycerol 90 mM CaCl2 in H20 | 50 | yes | 6.20E+05 | ||||||||||
7 | 4 | 06-26 | Charlotte Bunne, Anna, Jan | 66C1-2 | 7 | E. coli | Rosetta (DE3) | CaCl2 | 10% Glycerol 90 mM CaCl2 in H20 | 50 | 1.50E+05 | 3.10E+01 | ||||||||||
8 | 5 | 06-26 | Charlotte Bunne, Anna, Jan | 66C1-2 | 7 | E. coli | Rosetta (DE3) | CaCl2 | 10% Glycerol 90 mM CaCl2 in H20 | 50 | yes | 5.00E+04 | 3.10E+01 | douBIe concentration (only 0.5 volumes final resupension volume) | HC | |||||||
9 | 6 | 7/6/2014 | Jan | 134C1-2 | 7 | E. coli | Rosetta (DE3) | CaCl2 | 100 mM CaCl2 in H20 | 300 | yes | 31,30 | in situ competent cells in order to get N and C inteins both into the cells | |||||||||
10 | 7 | 7/6/2014 | Jan | 137C1-2 | 7 | E. coli | Rosetta (DE3) | CaCl2 | 100 mM CaCl2 in H20 | 300 | yes | 31,29 | in situ competent cells in order to get N and C inteins both into the cells | |||||||||
11 | 8 | 7/12/2014 | Silvan | 12C1 | 6 | E. coli | BI21(DE3) | CaCl2 | 10% Glycerol 90 mM CaCl2 in H20 | yes | in situ | |||||||||||
12 | 9 | 7/20/2014 | Silvan | 12C2-1 | 6 | E. coli | BI21(DE3) | CaCl2 | 10% Glycerol 90 mM CaCl2 in H20 | 50 | yes | 1.80E+04 | < 2000 | |||||||||
13 | 10 | 7/28/2014 | Charlotte | 12C3-1 | 200 | 6 | E. coli | BI21(DE3) | CaCl2 | 5 | 10% Glycerol 90 mM CaCl2 in H20 | 100 | yes | 8.00E+05 | RFC99,w/o liquid nitrogen and cold room | |||||||
14 | 11 | 07-31 | Jan | 6C1-2 | 250 | 6 | E. coli | BI21(DE3) | CaCl2 | 1 | 15% Glycerol 100 mM CaCl2 in H20 | 50 | yes | 1.50E+03 | ||||||||
15 | 12 | 07-31 | Jan | 6C1-3 | 250 | 6 | E. coli | BI21(DE3) | CaCl2 | 1 | 15% Glycerol 100 mM CaCl2 in H20 | 50 | yes | 2.00E+03 | ||||||||
16 | 13 | 7/30/2014 | Sabine | 1 | E. coli | TOP10 | 50 | yes | 5.00E+06 | w/o liquid nitrogen and cold room in special eppis | ||||||||||||
17 | 14 | 08-02 | Charlotte | 3C1-2 | 100 | 3 | E. coli | Turbos | CaCl2 | 15% Glycerol 100 mM CaCl2 in H20 | 100 | yes | ||||||||||
18 | 15 | 08-12 | Charlotte | 3C1-3 | 50 | 3 | E. coli | Turbos | CaCl2 | 15% Glycerol 100 mM CaCl2 in H20 | 50 | yes | 1.38E+07 | |||||||||
19 | 16 | 8/30/2014 | Charlotte | 3C1-5 | 200 | 3 | E. coli | Turbos | CaCl2 | 15% Glycerol 100 mM CaCl2 in H20 | 80 | yes | 3.40E+06 | |||||||||
20 | 17 | 9/8/2014 | Charlotte, Silvan | 6C1-11 | 200 | 6 | E. coli | BI21(DE3) | CaCl2 | 5 | 15% Glycerol 100 mM CaCl2 in H20 | 80 | 1.65E+04 | RFC99,w/o liquid nitrogen and cold room | 1.00E+04 | |||||||
21 | 18 | 9/8/2014 | Charlotte | 3C1-6 | 200 | 3 | E. coli | Turbos | CaCl2 | 5 | 15% Glycerol 100 mM CaCl2 in H20 | 80 | yes | 3.50E+06 | RFC99,w/o liquid nitrogen and cold room | 8.00E+05 | ||||||
22 | 19 | 9/9/2014 | Charlotte, Jan | 6C1-13 | 200 | 6 | E. coli | BI21(DE3) | CaCl2 | 5 | 10% Glycerol 90 mM CaCl2 in H20 | 100 | 6.00E+04 | RFC99,w/o liquid nitrogen and cold room | 6.00E+04 | |||||||
23 | 20 | 9/15/2014 | Charlotte | 3C1-7 | 200 | 3 | E. coli | Turbos | CaCl2 | 5 | 10% Glycerol 90 mM CaCl2 in H20 | 80 | yes | 8.00E+05 | 8.00E+05 | |||||||
24 | 21 | 9/25/2014 | Florian Schmidt | 1 | E. coli | TOP10 | Inoue | yes | ||||||||||||||
25 | 22 | 9/27/2014 | Jan | 3C1-9 | 100 | 3 | E. coli | Turbos | CaCl2 | 3 | 10% Glycerol 90 mM CaCl2 in H20 | 100 | 1.40E+06 | RFC99,w/o liquid nitrogen and cold room | 1.40E+06 | 6.05E+04 | ||||||
26 | 23 | 9/27/2014 | Jan | 3C1-10 | 200 | 3 | E. coli | Turbos | CaCl2 | 5 | 10% Glycerol 90 mM CaCl2 in H20 | 100 | yes | 1.60E+06 | RFC99,w/o liquid nitrogen and cold room | 1.60E+06 | ||||||
27 | 24 | 9/28/2014 | Charlotte | 6C1-18 | 200 | 6 | E. coli | BI21(DE3) | CaCl2 | 10% Glycerol 90 mM CaCl2 in H21 | 80 | |||||||||||
28 | 25 | 10/1/2014 | Jan | 3C1-13 | 200 | 3 | E. coli | Turbos | Inoue like | 20 | 1%(v/v) DMSO in TB (Inoue II) | 100 | cells were grown at 37°C not 18°C, some aliquots are 50 and some 80 | 2.36E+05 | ||||||||
29 | 26 | 10/1/2014 | Jan | 3C1-13 | 200 | 3 | E. coli | Turbos | CaCl2 | 5 | 10% Glycerol 90 mM CaCl2 in H21 | 100 | first cetrifugation was just 10 min, both centrifugations were at just 2500g | 1.39E+05 | ||||||||
30 | 27 | #N/A | #N/A | |||||||||||||||||||
31 | 28 | #N/A | #N/A | |||||||||||||||||||
32 | 29 | #N/A | #N/A | |||||||||||||||||||
33 | 30 | #N/A | #N/A | |||||||||||||||||||
34 | 31 | #N/A | #N/A | |||||||||||||||||||
35 | 32 | #N/A | #N/A | |||||||||||||||||||
36 | 33 | #N/A | #N/A | |||||||||||||||||||
37 | 34 | #N/A | #N/A | |||||||||||||||||||
38 | 35 | #N/A | #N/A | |||||||||||||||||||
39 | 36 | #N/A | #N/A | |||||||||||||||||||
40 | 37 | #N/A | #N/A | |||||||||||||||||||
