=Thursday 11th september
Lab Work
By Mélanie
Lemon Scent
LS and GS digestion
As I failed to obtain the insert with a kit purification, we try another methode with the plasmid topo LS and GS:
| component
| volume
|
| H2O
| 6μl
|
| buffer
| 2μl
|
| PacI and MscI
| 1μl
|
| Plasmid
| 10μl
|
LS and GS ligation
| component
| volume
|
| insert
| 7μl
|
| buffer
| 1μl
|
| ligase
| 1μl
|
| pPSI
| 1μl
|
ligation of PS in TOPO vector and transformation
| component
| volume
|
| H2O
| 3μl
|
| salt
| 1μl
|
| PCR product
| 1μl
|
| TOPO vector
| 1μl
|
Transformation in bacteria
Preparation of pSCBIC3 plasmid
I use the protocol iGEM/
| component
| volume
|
| H2O
| 9μl
|
| buffer
| 2μl
|
| ecoRI and PSTI and dpnI
| 1μl
|
| Plasmid
| 8μl
|
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