Team:Paris Saclay/Notebook/September/16

Lab Work

By Mélanie

D- Lemon scent

Plasmids extraction

Extraction of the plasmid from the culture made yesterday(PS)

Digestion of PS topo plasmids

by PacI and PSTI

(well 2 and 3 : PacI digestion)

Ligation

After digestion, ligation overnight in PPS1

PCR Colony

Test of the transformation made yesterday to know if we had obtained a plasmid]

primer to ckeck the direction of the insert PPS2 : iPS72-96

PPS4 : ips72-97

= no insert (just well 1-9)

liquide culture

liquide culture from yesterday's colony to make stock tomorrow

PCR cad to cloning in pSBIC3

PCR and digestion PSTI/ecoRI and ligation

PCR GS

PCR to check if we have the insert in our topo plasmid

see here

B- Construction of the fusion protein (color)

PCR

PCR to check if we have the insert in our topo plasmid

see here

well 2-9 = chromo yellow in topo plasmid well 10-17 = chromo blue in topo plasmid