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Team:Paris Saclay/Notebook/September/11
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Contents |
Lab Work
By Mélanie
D-Lemon Scent
LS and GS digestion
As I failed to obtain the insert with a kit purification, we try another methode with the plasmid topo LS and GS:
| component | volume |
|---|---|
| H2O | 6μl |
| buffer | 2μl |
| PacI and MscI | 1μl |
| Plasmid | 10μl |
LS and GS ligation
| component | volume |
|---|---|
| insert | 7μl |
| buffer | 1μl |
| ligase | 1μl |
| pPSI | 1μl |
ligation of PS in TOPO vector and transformation
| component | volume |
|---|---|
| H2O | 3μl |
| salt | 1μl |
| PCR product | 1μl |
| TOPO vector | 1μl |
Transformation in bacteria
Preparation of pSCBIC3 plasmid
I use the protocol iGEM/
| component | volume |
|---|---|
| H2O | 9μl |
| buffer | 2μl |
| ecoRI and PSTI and dpnI | 1μl |
| Plasmid | 8μl |
