Team:Paris Saclay/Notebook

From 2014.igem.org

(Difference between revisions)
(→D - Lemon scent)
(→F - The lemon shaping)
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*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 7th August]
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7 7th August]
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 8th August]
*Agar mold test - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8 8th August]
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*Incubation of E. Coli with plasmid FNR RBS AmylCP [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11 11th August]
+
*Incubation of E. Coli with plasmid FNR RBS AmylCP in LB [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11 11th August]
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 12th August]
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/12 12th August]
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13 13th August]
*Coloration of agar [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/13 13th August]
*Preparation of M63 medium [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/14 14th August]
*Preparation of M63 medium [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/14 14th August]
 +
* Incubation of E. Coli with plasmid FNR RBS AmylCP in M63 [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/18#Monday_18th_August 18th August]

Revision as of 15:13, 25 August 2014

Contents

Notebook

We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page

Planning

A - The chassis coli Odor free

  • Culture of the supercompetent cells MG1655 and MG1655Z1 - 30th June
  • Phages preparation for the transduction - 2nd July
  • Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 - 3rd July
  • Cultures of MG1655 and MG1655Z1 - 4th July
  • Transformations test of competent cells MG1655 and MG1655Z1 - 10th July
  • Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - 11th July
  • Preparation of competent cells E. coli MG1655 and MG1655Z1 - 17th July
  • Transformation of supercompetent cells MG1655 and MG1655Z1 - 18th July
  • Results of the transformation - 21st July
  • Preparation and transformation of competent MG1655 and MG1655Z1 - 22nd July
  • PCR verification of the strains grown - 25th July
  • Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - 28th July
  • PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - 29th July
  • Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 - 30th July
  • Stria of MG1655 and MG1655Z1 on LB dishes - 1st August
  • Stria of MG1655 and MG1655Z1 on LB and kan dishes - 4th August
  • PCR verification of the final strains MG1655 and MG1655Z1 - 5th August
  • Final stock of MG1655 - 6th August

B - Construction of the fusion protein (color)

C - Salicylate Inducible Suppressing System

D - Lemon scent

  • Rehydration of BioBricks BBa_J45014, BBa_K517003 - 30th June
  • Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - 18th July
  • Extraction of p cola plasmid DNA - 22nd July
  • PCR targeting with DY330 and pJBEI-6409 - 22nd July
  • Extraction and electrophoresis of BBa_K762100 with pSB1C3 - 24th July
  • Gel electrophoresis of p cola - 24th July
  • Transformation of DY330 with pJBEI-6409 - 25th July
  • Liquid culture of DY330 - 28th July
  • Transformation of DY330 with pJBEI-6409 - 29th July
  • PCR of BBa_K762100 - 29th July
  • PCR of BBa_K762100 - 30th July
  • Culture of DY330 + pJBEI-6409 - 31st July
  • Transformation of DY330 with pJBEI-6409 - 1st August
  • Electroporation of DY330+pJBEI-6409 - 4th August
  • PCR of BBa_517003 and BBa_K762100 - 4th August
  • Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
  • PCR cleanup of BBa_K517003 and BBa_K762100 - 6th August
  • PCR of BBa_517003 and BBa_K762100 - 7th August
  • PCR Clean-up of BBa_K762100 - 11th August
  • PCR Clean-up of p cola and BBa_K517003 - 12th August
  • Gel electrophoresis of p cola, BBa_K762100 and BBa_K517003 - 12th August
  • Gel electrophoresis of BBa_K762100 - 13th August
  • Cloning BBa_K517003 and p cola in pGEMTeasy - 13th August
  • PCR of BBa_K517003 - 18th August
  • PCR of BBa_K762100 - 18th August
  • Transformation of DH5α with CAD and chromoprotein - 21st August

E - Banana scent

F - The lemon shaping

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