Thursday 10th July

Lab work

Plasmid DNA Extraction

by Romain and Sean

Plasmids used:

• BBa_B0015, dble ter
• FNR_RBS_amilCP-Ter DH5αZ1

Protocol

The chassis coli Odor free

Transformation of competent cells E.coli

by Fabio and Maher

Competent strains used:

• DH5α
• MG1655
• MG1655Z1
• DH5α+ (given by Solenne)

(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu)

BioBricks used:

• BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
• BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)
• BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10^-1)

+ pET28a (Kan R)(Dilutions: 0, 10^-1 and 10^-2) (pET28a is a plasmid used to verify that the 4 strains are competent).

Negative control groups:

• pET28a: without plasmid, nothing grows.
• BBa_J23100, BBa_J23106, BBa_J23114: without the BioBrick, nothing grows.

Results:

• pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
• BBa_J23100, BBa_J23106, BBa_J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.

Protocol

Modeling

by Arnaud, Eugene, Juliette and Pierre

Discussion around differents modeling possibilities in concertation with Solenne.

Photo of the Day

Members present:

• Instructors and advisors: Alice, Solenne and Sylvie.
• Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar