Team:Paris Saclay/Notebook

From 2014.igem.org

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===D - Lemon Scent===
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* PCR of BBa_K762100 [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#PCR_of_BBa_K762100 30th July]
* Culture of DY330 + pJBEI-6409 [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent 31st July]
* Culture of DY330 + pJBEI-6409 [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#D_-_Lemon_scent 31st July]
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells 1st August]
* Transformation of DY330 with pJBEI-6409 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#New_Transformation_of_electrocompetent_cells 1st August]

Revision as of 19:02, 7 August 2014

Contents

Notebook

We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.

Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page

Planning

A - The chassis coli Odor free

  • Culture of the supercompetent cells MG1655 and MG1655Z1 - 30th June
  • Phages preparation for the transduction - 2nd July
  • Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 - 3rd July
  • Cultures of MG1655 and MG1655Z1 - 4th July
  • Transformations test of competent cells MG1655 and MG1655Z1 - 10th July
  • Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - 11th July
  • Preparation of competent cells E. coli MG1655 and MG1655Z1 - 17th July
  • Transformation of supercompetent cells MG1655 and MG1655Z1 - 18th July
  • Results of the transformation - 21st July
  • Preparation and transformation of competent MG1655 and MG1655Z1 - 22nd July
  • PCR verification of the strains grown - 25th July
  • Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - 28th July
  • PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - 29th July
  • Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 - 30th July
  • Stria of MG1655 and MG1655Z1 on LB dishes - 1st August
  • Stria of MG1655 and MG1655Z1 on LB and kan dishes - 4th August
  • PCR verification of the final strains MG1655 and MG1655Z1 - 5th August
  • Final stock of MG1655 - 6th August

C - Salicylate Inducible Suppressing System

  • Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23rd July
  • Transformation of competent E.coli cells - 24th July
  • Bacterial culture - 25th July
  • Plasmid DNA Purification - 28th July
  • Digestion to check & Electrophoresis - 28th July
  • Main Digestion - 29th July
  • Segregate Process - 29th July
  • DNA extraction from gel agarose - 30th July
  • Ligation - 30th July
  • Transformation of competent E.coli cells - 31st July
  • Liquid Culture - 1st August
  • Plasmid DNA Purification - 4th August
  • Digestion to check & Electrophoresis - 4th August
  • Final Stock BBa_K1372000 - 4th August
  • Liquid Culture - 5th August
  • Plasmid DNA Purification - 6th August
  • Digestion of BBa_B0015 and BBa_K1372000 - 6th August
  • Segregate Process - 6th August
  • DNA extraction from gel agarose - 7th August
  • Ligation - 7th August
  • Transformation of competent E.coli cells - 8th August
  • Liquid Culture - 11th August
  • Plasmid DNA Purification - 12th August
  • Digestion to check & Electrophoresis - 12th August
  • Final Stock - 12th August

D - Lemon Scent

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