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Team:Paris Saclay/Notebook/July/10
From 2014.igem.org
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=Thursday 10th July= | =Thursday 10th July= | ||
==Lab work== | ==Lab work== | ||
| - | ===Plasmid DNA Extraction=== | + | ====Plasmid DNA Extraction==== |
''by Romain and Sean'' | ''by Romain and Sean'' | ||
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | ||
| - | ===Transformation of competent cells E.coli=== | + | ===A - The chassis coli Odor free=== |
| + | ====Transformation of competent cells E.coli==== | ||
''by Fabio and Maher'' | ''by Fabio and Maher'' | ||
Revision as of 14:28, 7 August 2014
Contents |
Thursday 10th July
Lab work
Plasmid DNA Extraction
by Romain and Sean
Plasmids used:
- BBa_B0015, dble ter
- FNR_RBS_amilCP-Ter DH5αZ1
A - The chassis coli Odor free
Transformation of competent cells E.coli
by Fabio and Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu)
BioBricks used:
- BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
- BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
- BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
+ pET28a (Kan R)(Dilutions: 0, 10-1 and 10-2) (pET28a is a plasmid used to verify that the 4 strains are competent).
Negative control groups:
- pET28a: without plasmid, nothing grows.
- BBa_J23100, BBa_J23106, BBa_J23114: without the BioBrick, nothing grows.
Results:
- pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
- BBa_J23100, BBa_J23106, BBa_J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
Modeling
by Arnaud, Eugene, Juliette and Pierre
Discussion around differents modeling possibilities in concertation with Solenne.
