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Team:Paris Saclay/Notebook/September/16
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==Lab Work== | ==Lab Work== | ||
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well 2-9 = chromo yellow in topo plasmid | well 2-9 = chromo yellow in topo plasmid | ||
well 10-17 = chromo blue in topo plasmid | well 10-17 = chromo blue in topo plasmid | ||
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Revision as of 19:47, 29 September 2014
Contents |
Lab Work
By Mélanie
D- Lemon scent
Plasmids extraction
Extraction of the plasmid from the culture made yesterday(PS)
Digestion of PS topo plasmids
by PacI and PSTI
(well 2 and 3 : PacI digestion)
Ligation
After digestion, ligation overnight in PPS1
PCR Colony
Test of the transformation made yesterday to know if we had obtained a plasmid]
primer to ckeck the direction of the insert PPS2 : iPS72-96
PPS4 : ips72-97
= no insert (just well 1-9)
liquide culture
liquide culture from yesterday's colony to make stock tomorrow
PCR cad to cloning in pSBIC3
PCR and digestion PSTI/ecoRI and ligation
PCR GS
PCR to check if we have the insert in our topo plasmid
B- Construction of the fusion protein (color)
PCR
PCR to check if we have the insert in our topo plasmid
well 2-9 = chromo yellow in topo plasmid well 10-17 = chromo blue in topo plasmid
