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Team:Paris Saclay/Notebook
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==Planning== | ==Planning== | ||
===A - The ''E. coli'' odor free chassis=== | ===A - The ''E. coli'' odor free chassis=== | ||
| - | *Culture of | + | *Culture of MG1655 and MG1655Z1 strains- [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June] |
| - | * | + | *P1 phage stock preparation for the transduction of the ''Delta-tnaA::Kan'' - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July] |
| - | * | + | *P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 3rd July] |
| - | *Cultures of MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 4th July] | + | *Cultures of MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 4th July] |
*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 10th July] | *Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 10th July] | ||
*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 11th July] | *Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 11th July] | ||
| - | *Preparation of competent cells E. coli MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July] | + | *Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July] |
| - | *Transformation of supercompetent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July] | + | *Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with pCP20- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July] |
*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July] | *Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July] | ||
| - | *Preparation and transformation of competent MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 22nd July] | + | *Preparation and transformation of competent MG1655 and MG1655Z1 transductants - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 22nd July] |
*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 25th July] | *PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 25th July] | ||
*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 28th July] | *Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 28th July] | ||
Revision as of 17:42, 18 September 2014
Contents |
Notebook
We constituted our team in November 2013 and we had meetings twice a month until April. Then we met every week. The Lab work started in the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page
Planning
A - The E. coli odor free chassis
- Culture of MG1655 and MG1655Z1 strains- 30th June
- P1 phage stock preparation for the transduction of the Delta-tnaA::Kan - 2nd July
- P1 phage transduction using the stock prepared on July 2nd to MG1655 and MG1655Z1 strains - 3rd July
- Cultures of MG1655 and MG1655Z1 transductants - 4th July
- Transformations test of competent cells MG1655 and MG1655Z1 - 10th July
- Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - 11th July
- Preparation of competent cells E. coli MG1655 and MG1655Z1 transductants - 17th July
- Transformation of supercompetent cells MG1655 and MG1655Z1 transductants with pCP20- 18th July
- Results of the transformation - 21st July
- Preparation and transformation of competent MG1655 and MG1655Z1 transductants - 22nd July
- PCR verification of the strains grown - 25th July
- Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - 28th July
- PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - 29th July
- Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 - 30th July
- Stria of MG1655 and MG1655Z1 on LB dishes - 1st August
- Stria of MG1655 and MG1655Z1 on LB and kan dishes - 4th August
- PCR verification of the final strains MG1655 and MG1655Z1 - 5th August
- Final stock of MG1655 - 6th August
B - Construction of the fusion protein (color)
C - Salicylate Inducible Suppressing System
- Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23rd July
- Transformation of competent E.coli cells - 24th July
- Bacterial culture - 25th July
- Plasmid DNA Purification - 28th July
- Digestion to check & Electrophoresis - 28th July
- Main Digestion of BBa_J61051 and BBa_K228001 - 29th July
- Segregate Process - 29th July
- DNA purification gel agarose - 30th July
- Ligation - 30th July
- Transformation of competent E.coli cells - 31st July
- Liquid Culture - 1st August
- Plasmid DNA Purification - 4th August
- Digestion to check & Electrophoresis - 4th August
- Final Stock BBa_K1372000 - 4th August
- Liquid Culture - 5th August
- Plasmid DNA Purification - 6th August
- Digestion of BBa_K1372000 and BBa_B0015- 6th August
- Segregate Process - 6th August
- DNA purification gel agarose - 7th August
- Ligation - 7th August
- Transformation of competent E.coli cells - 8th August
- Liquid Culture - 11th August
- Plasmid DNA Purification - 12th August
- Digestion to check & Electrophoresis - 12th August
- Final Stock BBa_K1372001 - 12th August
D - Lemon scent
- Rehydration of BioBricks BBa_J45014, BBa_K517003 - 30th June
- Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - 18th July
- Extraction of p cola plasmid DNA - 22nd July
- PCR targeting with DY330 and pJBEI-6409 - 22nd July
- Extraction and electrophoresis of BBa_K762100 with pSB1C3 - 24th July
- Gel electrophoresis of p cola - 24th July
- Transformation of DY330 with pJBEI-6409 - 25th July
- Liquid culture of DY330 - 28th July
- Transformation of DY330 with pJBEI-6409 - 29th July
- PCR of BBa_K762100 - 29th July
- PCR of BBa_K762100 - 30th July
- Culture of DY330 + pJBEI-6409 - 31st July
- Transformation of DY330 with pJBEI-6409 - 1st August
- Electroporation of DY330+pJBEI-6409 - 4th August
- PCR of BBa_517003 and BBa_K762100 - 4th August
- Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
- PCR cleanup of BBa_K517003 and BBa_K762100 - 6th August
- PCR of BBa_517003 and BBa_K762100 - 7th August
- PCR Clean-up of BBa_K762100 - 11th August
- PCR Clean-up of p cola and BBa_K517003 - 12th August
- Gel electrophoresis of p cola, BBa_K762100 and BBa_K517003 - 12th August
- Gel electrophoresis of BBa_K762100 - 13th August
- Cloning BBa_K517003 and p cola in pGEMTeasy - 13th August
- PCR of BBa_K517003 - 18th August
- PCR of BBa_K762100 - 18th August
- Transformation of DH5α with CAD and chromoprotein - 21st August
- Extraction of pPS3 and pPS4 - 26th August
E - Banana scent
F - The lemon shaping
- Concentration Agar Test - 30th June
- Concentration Agar Test - 5th August
- Agar mold test - 7th August
- Agar mold test - 8th August
- Incubation of E. Coli with plasmid FNR RBS AmylCP in LB 11th August
- Coloration of agar 12th August
- Coloration of agar 13th August
- Preparation of M63 medium 14th August
- Incubation of E. Coli with plasmid FNR RBS AmylCP in M63 18th August
- Transformation of odor free E. coli with plasmids coding Fluo Protein 27th August
- Results of Fluorescent Protein 29th August
