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Team:Paris Saclay/Notebook/July/10
From 2014.igem.org
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* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
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Latest revision as of 15:38, 14 October 2014
Contents |
Thursday 10th July
Lab work
Plasmid DNA Extraction
by Romain and Sean
Plasmids used:
- BBa_B0015, dble ter
- FNR_RBS_amilCP-Ter DH5αZ1
The chassis coli Odor free
Transformation of competent cells E.coli
by Fabio and Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu)
BioBricks used:
- BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
- BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
- BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
+ pET28a (Kan R)(Dilutions: 0, 10-1 and 10-2) (pET28a is a plasmid used to verify that the 4 strains are competent).
Negative control groups:
- pET28a: without plasmid, nothing grows.
- BBa_J23100, BBa_J23106, BBa_J23114: without the BioBrick, nothing grows.
Results:
- pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
- BBa_J23100, BBa_J23106, BBa_J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
Modeling
by Arnaud, Eugene, Juliette and Pierre
Discussion around differents modeling possibilities in concertation with Solenne.
Photo of the Day
Members present:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
