Team:Paris Saclay/Notebook/September/18
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+ | {{Team:Paris_Saclay/notebook_header}} | ||
+ | =Thursday 18th September= | ||
+ | |||
==Lab Work== | ==Lab Work== | ||
''By Mélanie'' | ''By Mélanie'' | ||
+ | |||
+ | ===Lemon scent=== | ||
+ | |||
+ | ====PCR with colony==== | ||
+ | |||
+ | clones obtained after the ligation in PPSI have been tested by PCR to see if we have an insert: | ||
+ | We have tested pps2-pps3 pps4. | ||
+ | |||
+ | |||
+ | Only pps4 is positive for one clone and pps3 for one. | ||
+ | |||
+ | [[File:1809 1-10 pps2 10-16 pps3.jpg|400px|center]] | ||
+ | |||
+ | well 2-11 : pPS2 | ||
+ | |||
+ | well 12-16 : pPS3 | ||
+ | |||
+ | [[File:1809 pps3 17-20 21 pps4.jpg|400px|center]] | ||
+ | |||
+ | well 2-4 pPS3 | ||
+ | well 5 pPS4 | ||
+ | |||
+ | ====liquide culture==== | ||
+ | |||
+ | liquide culture of the positiv clones | ||
+ | |||
+ | ==Photo of the Day== | ||
+ | [[File:Paris Saclay 18_september.jpg|300px|center]] | ||
+ | |||
+ | {{Team:Paris_Saclay/notebook_footer}} |
Latest revision as of 13:16, 14 October 2014
Contents |
Thursday 18th September
Lab Work
By Mélanie
Lemon scent
PCR with colony
clones obtained after the ligation in PPSI have been tested by PCR to see if we have an insert: We have tested pps2-pps3 pps4.
Only pps4 is positive for one clone and pps3 for one.
well 2-11 : pPS2
well 12-16 : pPS3
well 2-4 pPS3 well 5 pPS4
liquide culture
liquide culture of the positiv clones