"
Team:Paris Saclay/Notebook
From 2014.igem.org
(Difference between revisions)
(→C - Salicylate Inducible Suppressing System) |
(→C - Salicylate Inducible Suppressing System) |
||
| Line 45: | Line 45: | ||
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July] | *Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July] | ||
*Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July] | *Digestion to check & Electrophoresis - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28#C_-_Salicylate_Inducible_Suppressing_System 28th July] | ||
| - | * | + | *Main Digestion of BBa_J61051 and BBa_K228001 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July] |
| - | + | *Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29#C_-_Salicylate_Inducible_Suppressing_System 29th July] | |
| - | + | *DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | |
| - | + | *Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30#C_-_Salicylate_Inducible_Suppressing_System 30th July] | |
| - | + | ||
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July] | *Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#C_-_Salicylate_Inducible_Suppressing_System 31st July] | ||
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#C_-_Salicylate_Inducible_Suppressing_System 1st August] | *Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1#C_-_Salicylate_Inducible_Suppressing_System 1st August] | ||
| Line 57: | Line 56: | ||
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#C_-_Salicylate_Inducible_Suppressing_System 5th August] | *Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5#C_-_Salicylate_Inducible_Suppressing_System 5th August] | ||
*Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August] | *Plasmid DNA Purification - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August] | ||
| - | * | + | *Digestion of BBa_K1372000 and BBa_B0015- [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August] |
| - | + | *Segregate Process - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6#C_-_Salicylate_Inducible_Suppressing_System 6th August] | |
| - | + | *DNA purification gel agarose - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#C_-_Salicylate_Inducible_Suppressing_System 7th August] | |
| - | + | *Ligation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/7#C_-_Salicylate_Inducible_Suppressing_System 7th August] | |
| - | + | ||
*Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#C_-_Salicylate_Inducible_Suppressing_System 8th August] | *Transformation of competent E.coli cells - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/8#C_-_Salicylate_Inducible_Suppressing_System 8th August] | ||
*Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#C_-_Salicylate_Inducible_Suppressing_System 11th August] | *Liquid Culture - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/11#C_-_Salicylate_Inducible_Suppressing_System 11th August] | ||
Revision as of 16:57, 12 August 2014
Contents |
Notebook
We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.
Use our calendar to have a look on our notebook and our filter to go directly to Lab Work, Reunion, Human Practices or Modeling work. Click here to edit this page
Planning
A - The chassis coli Odor free
- Culture of the supercompetent cells MG1655 and MG1655Z1 - 30th June
- Phages preparation for the transduction - 2nd July
- Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 - 3rd July
- Cultures of MG1655 and MG1655Z1 - 4th July
- Transformations test of competent cells MG1655 and MG1655Z1 - 10th July
- Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - 11th July
- Preparation of competent cells E. coli MG1655 and MG1655Z1 - 17th July
- Transformation of supercompetent cells MG1655 and MG1655Z1 - 18th July
- Results of the transformation - 21st July
- Preparation and transformation of competent MG1655 and MG1655Z1 - 22nd July
- PCR verification of the strains grown - 25th July
- Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - 28th July
- PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - 29th July
- Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 - 30th July
- Stria of MG1655 and MG1655Z1 on LB dishes - 1st August
- Stria of MG1655 and MG1655Z1 on LB and kan dishes - 4th August
- PCR verification of the final strains MG1655 and MG1655Z1 - 5th August
- Final stock of MG1655 - 6th August
B - Construction of the fusion protein (color)
C - Salicylate Inducible Suppressing System
- Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23rd July
- Transformation of competent E.coli cells - 24th July
- Bacterial culture - 25th July
- Plasmid DNA Purification - 28th July
- Digestion to check & Electrophoresis - 28th July
- Main Digestion of BBa_J61051 and BBa_K228001 - 29th July
- Segregate Process - 29th July
- DNA purification gel agarose - 30th July
- Ligation - 30th July
- Transformation of competent E.coli cells - 31st July
- Liquid Culture - 1st August
- Plasmid DNA Purification - 4th August
- Digestion to check & Electrophoresis - 4th August
- Final Stock BBa_K1372000 - 4th August
- Liquid Culture - 5th August
- Plasmid DNA Purification - 6th August
- Digestion of BBa_K1372000 and BBa_B0015- 6th August
- Segregate Process - 6th August
- DNA purification gel agarose - 7th August
- Ligation - 7th August
- Transformation of competent E.coli cells - 8th August
- Liquid Culture - 11th August
- Plasmid DNA Purification - 12th August
- Digestion to check & Electrophoresis - 12th August
- Final Stock BBa_K1372001 - 12th August
D - Lemon scent
- Rehydration of BioBricks BBa_J45014, BBa_K517003 - 30th June
- Preparation of electrocompetent DY330 and transformation via pJBEI-6409 - 18th July
- Extraction of p cola plasmid DNA - 22nd July
- PCR targeting with DY330 and pJBEI-6409 - 22nd July
- Extraction and electrophoresis of BBa_K762100 with pSB1C3 - 24th July
- Gel electrophoresis of p cola - 24th July
- Transformation of DY330 with pJBEI-6409 - 25th July
- Liquid culture of DY330 - 28th July
- Transformation of DY330 with pJBEI-6409 - 29th July
- PCR of BBa_K762100 - 29th July
- PCR of BBa_K762100 - 30th July
- Culture of DY330 + pJBEI-6409 - 31st July
- Transformation of DY330 with pJBEI-6409 - 1st August
- Electroporation of DY330+pJBEI-6409 - 4th August
- PCR of BBa_517003 and BBa_K762100 - 4th August
- Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
- PCR cleanup of BBa_K517003 and BBa_K762100 - 6th August
- PCR of BBa_517003 and BBa_K762100 - 7th August
- PCR Clean-up of BBa_K762100 - 11th August
E - Banana scent
F - The lemon shaping
- Concentration Agar Test - 30th June
- Concentration Agar Test - 5th August
- Agar mold test - 7th August
- Agar mold test - 8th August
