"
Team:Paris Saclay/Notebook/July/10
From 2014.igem.org
(Difference between revisions)
(→2 - Transformation of competent cells E.coli) |
m (→Thursday 10th July) |
||
| (9 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{Team:Paris_Saclay/notebook_header}} | ||
=Thursday 10th July= | =Thursday 10th July= | ||
| - | |||
==Lab work== | ==Lab work== | ||
| - | + | ====Plasmid DNA Extraction==== | |
| - | === | + | |
| - | + | ||
''by Romain and Sean'' | ''by Romain and Sean'' | ||
| Line 13: | Line 11: | ||
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Protocol] | ||
| - | === | + | ===The chassis coli Odor free=== |
| - | + | ====Transformation of competent cells E.coli==== | |
''by Fabio and Maher'' | ''by Fabio and Maher'' | ||
| Line 25: | Line 23: | ||
BioBricks used: | BioBricks used: | ||
| - | * | + | *BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10<sup>-1</sup>) |
| - | * | + | *BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10<sup>-1</sup>) |
| - | * | + | *BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10<sup>-1</sup>) |
+ pET28a (Kan R)(Dilutions: 0, 10<sup>-1</sup> and 10<sup>-2</sup>) | + pET28a (Kan R)(Dilutions: 0, 10<sup>-1</sup> and 10<sup>-2</sup>) | ||
(pET28a is a plasmid used to verify that the 4 strains are competent). | (pET28a is a plasmid used to verify that the 4 strains are competent). | ||
| Line 33: | Line 31: | ||
Negative control groups: | Negative control groups: | ||
*pET28a: without plasmid, nothing grows. | *pET28a: without plasmid, nothing grows. | ||
| - | * | + | *BBa_J23100, BBa_J23106, BBa_J23114: without the BioBrick, nothing grows. |
'''Results:''' | '''Results:''' | ||
*pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well. | *pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well. | ||
| - | * | + | *BBa_J23100, BBa_J23106, BBa_J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated. |
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol] | ||
| - | [ | + | ==Modeling== |
| + | ''by Arnaud, Eugene, Juliette and Pierre'' | ||
| + | |||
| + | Discussion around differents modeling possibilities in concertation with Solenne. | ||
| + | |||
| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 10_july.jpg|600px|center]] | ||
| + | |||
| + | '''Members present:''' | ||
| + | * Instructors and advisors: Alice, Solenne and Sylvie. | ||
| + | * Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean. | ||
| + | |||
| + | |||
| + | {{Team:Paris_Saclay/notebook_footer}} | ||
Latest revision as of 15:38, 14 October 2014
Contents |
Thursday 10th July
Lab work
Plasmid DNA Extraction
by Romain and Sean
Plasmids used:
- BBa_B0015, dble ter
- FNR_RBS_amilCP-Ter DH5αZ1
The chassis coli Odor free
Transformation of competent cells E.coli
by Fabio and Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
(The strains DH5α, MG1655 and MG1655Z1 were prepared by Mathias and Mathieu)
BioBricks used:
- BBa_J23100 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
- BBa_J23106 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
- BBa_J23114 (Amp R)(Dilutions: Concentrated, 0 and 10-1)
+ pET28a (Kan R)(Dilutions: 0, 10-1 and 10-2) (pET28a is a plasmid used to verify that the 4 strains are competent).
Negative control groups:
- pET28a: without plasmid, nothing grows.
- BBa_J23100, BBa_J23106, BBa_J23114: without the BioBrick, nothing grows.
Results:
- pET28a: Nothing grows with the strains DH5α, MG1655, MG1655Z1. But the strain DH5α+ grows well.
- BBa_J23100, BBa_J23106, BBa_J23114: The strain DH5α+ grow well and the 3 others strains grow less. They have to be more concentrated.
Modeling
by Arnaud, Eugene, Juliette and Pierre
Discussion around differents modeling possibilities in concertation with Solenne.
Photo of the Day
Members present:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
