Team:Paris Saclay/Notebook/September/16

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=Tuesday 16th September=
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==Lab Work==
==Lab Work==
''By Mélanie''
''By Mélanie''

Revision as of 06:48, 14 October 2014

Contents

Tuesday 16th September

Lab Work

By Mélanie

D- Lemon scent

Plasmids extraction

Extraction of the plasmid from the culture made yesterday(PS)

Digestion of PS topo plasmids

by PacI and PSTI

1609 digestion ps et gs par pacI.jpg

(well 2 and 3 : PacI digestion)

Ligation

After digestion, ligation overnight in PPS1


PCR Colony

Test of the transformation made yesterday to know if we had obtained a plasmid]

1609 PCR sur clones.jpg

primer to ckeck the direction of the insert PPS2 : iPS72-96

PPS4 : ips72-97

= no insert (just well 1-9)

liquide culture

liquide culture from yesterday's colony to make stock tomorrow

PCR cad to cloning in pSBIC3

PCR and digestion PSTI/ecoRI and ligation

PCR GS

PCR to check if we have the insert in our topo plasmid

see here


B- Construction of the fusion protein (color)

PCR

PCR to check if we have the insert in our topo plasmid

see here

1609 chromo topo 2-9 jaune 10-17 bleu.jpg

well 2-9 = chromo yellow in topo plasmid well 10-17 = chromo blue in topo plasmid

Photo of the Day

Paris Saclay 2 september.jpg

Work of [http://ateliers-artistes-belleville.fr/artiste/therese-bichon/ Thérèse Bichon] , one of those few we have interviewed during this month of september.

To learn more about the ethic aspect of our project, just click here

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