Revision as of 08:55, 7 August 2014

Notebook

We constitute our team in November 2013 and we have meeting twice a month until April. Then we met every weeks. The Lab work started at the beginning of June.

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Planning

A - The chassis coli Odor free

Culture of the supercompetent cells MG1655 and MG1655Z1 - 30th June
Phages preparation for the transduction - 2nd July
Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 - 3rd July
Cultures of MG1655 and MG1655Z1 - 4th July
Transformations test of competent cells MG1655 and MG1655Z1 - 10th July
Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - 11th July
Preparation of competent cells E. coli MG1655 and MG1655Z1 - 17th July
Transformation of supercompetent cells MG1655 and MG1655Z1 - 18th July
Results of the transformation - 21st July
Preparation and transformation of competent MG1655 and MG1655Z1 - 22nd July
PCR verification of the strains grown - 25th July
Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - 28th July
PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - 29th July
Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 - 30th July
Stria of MG1655 and MG1655Z1 on LB dishes - 1st August
Stria of MG1655 and MG1655Z1 on LB and kan dishes - 4th August
PCR verification of the final strains MG1655 and MG1655Z1 - 5th August
Final stock of MG1655 - 6th August

C - Salicylate Inducible Suppressing System

Rehydration of BioBricks BBa_J61051 and BBa_K228001- 23th July
Transformation of competent E.coli cells - 24th July
Bacterial culture - 25th July
Plasmid DNA Purification - 28th July
Digestion to check & Electrophoresis - 28th July
Main Digestion - 29th July
Segregate Process - 29th July
DNA extraction from gel agarose - 30th July
Ligation - 30th July
Transformation of competent E.coli cells - 31st July
Liquid Culture - 1st August
Plasmid DNA Purification - 4th August
Digestion to check & Electrophoresis - 4th August
Final Stock BBa_K1372000 - 4th August
Liquid Culture - 5th August
Plasmid DNA Purification - 6th August
Digestion of BBa_B0015 and BBa_K1372000 - 6th August
Segregate Process - 6th August
DNA extraction from gel agarose - 7th August
Ligation - 7th August
Transformation of competent E.coli cells - 8th August
Liquid Culture - 11th August
Plasmid DNA Purification - 12th August
Digestion to check & Electrophoresis - 12th August
Final Stock - 12th August

D - Lemon Scent

PCR of BBa_517003 and BBa_K762100 - 4th August
Gel electrophoresis of BBa_K517003 and BBa_K762100 - 5th August
PCR Cleanup of BBa_K517003 and BBa_K762100 - 6th August

@@ Line 18: / Line 18: @@
 ==Planning==
 ===A - The chassis coli Odor free===
-*Culture of the supercompetent cells MG1655 and MG1655Z1 [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
+*Culture of the supercompetent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/June/30 30th June]
-*Phages preparation for the transduction [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July]
+*Phages preparation for the transduction - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/2 2nd July]
-*Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 [3rd July]
+*Phage transduction with the P1 phages in the strains MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/3 3rd July]
-*Cultures of MG1655 and MG1655Z1 [4th July]
+*Cultures of MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/4 4th July]
-*Transformations test of competent cells MG1655 and MG1655Z1 [10th July]
+*Transformations test of competent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/10 10th July]
-*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria [11th July]
+*Preparation of competent cells E coli, deletion of the antibiotic cassette in the odorless bacteria - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/11 11th July]
-*Preparation of competent cells E. coli MG1655 and MG1655Z1 [17th July]
+*Preparation of competent cells E. coli MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/17 17th July]
-*Transformation of supercompetent cells MG1655 and MG1655Z1 [18th July]
+*Transformation of supercompetent cells MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/18 18th July]
-*Results of the transformation [21st July]
+*Results of the transformation - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/21 21st July]
-*Preparation and transformation of competent MG1655 and MG1655Z1 [22nd July]
+*Preparation and transformation of competent MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/22 22nd July]
-*PCR verification of the strains grown [25th July]
+*PCR verification of the strains grown - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/25 25th July]
-*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 [28th July]
+*Preparation and Transformation of competent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/28 28th July]
-*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R [29th July]
+*PCR of MG1655 and MG1655Z1 with FTR-Apra-F and FTR-Apra-R - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/29 29th July]
-*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 [30th July]
+*Preparation and transformation of electrocompetent MG1655 and MG1655Z1 with BT340 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 30th July]
-*Stria of MG1655 and MG1655Z1 on LB dishes [1st August]
+*Stria of MG1655 and MG1655Z1 on LB dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/1 1st August]
-*Stria of MG1655 and MG1655Z1 on LB and kan dishes [4th August]
+*Stria of MG1655 and MG1655Z1 on LB and kan dishes - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 4th August]
-*PCR verification of the final strains MG1655 and MG1655Z1 [5th August]
+*PCR verification of the final strains MG1655 and MG1655Z1 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/5 5th August]
-*Final stock of MG1655 [6th August]
+*Final stock of MG1655 - [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/6 6th August]
 ===C - Salicylate Inducible Suppressing System===
 *Rehydration of BioBricks BBa_J61051 and BBa_K228001- [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23#C_-_The_Suppressing_Salicylate_System 23th July]

Team:Paris Saclay/Notebook

From 2014.igem.org