Team:Paris Saclay/Notebook/July/9

From 2014.igem.org

(Difference between revisions)
(→Wednesday 9th July)
m
 
(5 intermediate revisions not shown)
Line 1: Line 1:
 +
{{Team:Paris_Saclay/notebook_header}}
=Wednesday 9th July=
=Wednesday 9th July=
-
 
==Lab work==
==Lab work==
-
 
+
====Plasmid DNA Extraction====
-
====1 - Plasmid DNA Extraction:====
+
''by Romain and Sean''
-
 
+
-
'''Romain & Sean'''
+
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
Line 11: Line 9:
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
-
====2 - Electrophoresis:====
+
====Electrophoresis====
 +
''by Arnaud, Romain and Sean''
-
'''Arnaud & Romain & Sean'''
+
Plasmids used:
-
 
+
-
Plasmids used:  
+
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
Line 23: Line 20:
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
-
====3 - New culture:====
+
====New culture====
-
 
+
''by Romain''
-
'''Romain'''
+
New liquid cultures of:
New liquid cultures of:
Line 41: Line 37:
* Sponsoring
* Sponsoring
* Wiki's progress
* Wiki's progress
-
* Modelling's progress
+
* Modeling's progress
 +
==Photo of the Day==
 +
[[File:Paris Saclay 9_july.jpg|600px|center]]
-
Members present:
+
'''Members present:'''
* Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
* Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
-
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
+
{{Team:Paris_Saclay/notebook_footer}}

Latest revision as of 13:57, 17 October 2014

Contents

Wednesday 9th July

Lab work

Plasmid DNA Extraction

by Romain and Sean

Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.

Protocol

Electrophoresis

by Arnaud, Romain and Sean

Plasmids used:

  • DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
  • DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
  • XL1-Blue pCola-ges Kan
  • pJBEI-6409 Cm

Protocol

New culture

by Romain

New liquid cultures of:

  • FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
  • BBa B0015 dble ter pSB1C3 Cm

4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.

Reunion

Conference with the team to discuss about the project. The handled topics were:

  • Definition of Biobricks to make
  • Planning of internships
  • Definition of Biobricks to render
  • Understanding the construction of consecutive genes
  • Organisation and planning of experiences to do
  • Sponsoring
  • Wiki's progress
  • Modeling's progress

Photo of the Day

Paris Saclay 9 july.jpg

Members present:

  • Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/9"