Team:Paris Saclay/Notebook/August/5
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=Tuesday 5th August= | =Tuesday 5th August= | ||
==Lab work== | ==Lab work== | ||
+ | ===A - The chassis coli Odor free=== | ||
+ | ====PCR verification==== | ||
+ | ''by Romain'' | ||
+ | |||
+ | Results of the striates on dishes from [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/4 yesterday]: | ||
+ | |||
+ | For the strain MG1655Z1: | ||
+ | *Nothing has grown on the kan dish, but the colony grew on LB dish. That was what we expected, the kan cassette was correctly delete. | ||
+ | |||
+ | For the strain MG1655: | ||
+ | *Few colonies have grown on the kan dish, and all the colonies have grown on LB dish. It's a little failure. | ||
+ | |||
+ | So now, we proceed to the PCR to verify the length of the sequences. | ||
+ | |||
===C - Salicylate Inducible Suppressing System=== | ===C - Salicylate Inducible Suppressing System=== | ||
====Liquid Culture==== | ====Liquid Culture==== |
Revision as of 15:18, 5 August 2014
Contents |
Tuesday 5th August
Lab work
A - The chassis coli Odor free
PCR verification
by Romain
Results of the striates on dishes from yesterday:
For the strain MG1655Z1:
- Nothing has grown on the kan dish, but the colony grew on LB dish. That was what we expected, the kan cassette was correctly delete.
For the strain MG1655:
- Few colonies have grown on the kan dish, and all the colonies have grown on LB dish. It's a little failure.
So now, we proceed to the PCR to verify the length of the sequences.
C - Salicylate Inducible Suppressing System
Liquid Culture
by Fabio
2 liquid cultures of BBa_K1372000 with 20ml of LB and 20µl of amp (at 3pm - 150 rpm - 37 °C), from the stock made the 4st August.
D - Lemon scent
Electrophoresis
by Sean & Pierre The strains used were made the 4th August
- 5 µl of BBa_K762100 non diluted
- 5 µl of BBa_K762100 diluted 10-1
- 5 µl of BBa_K517003
Human Pratices
by Pierre
Ethics
I wrote the begining of a global view of the evolution of the definitions of life from Aristotle to modern times.
Bibliography
- Aristotle, On the Soul
- Epicurus, Letter to Herodotus
- Descartes, Discourse on the method
- Darwin, On the origin of species
- Bichat, Physiological researches on life and death
'By Hoang Vu' Continue my bibliographical research on Citral A and B and Beta-Pinene. I have now a little more than twenty reviews about various uses and properties of citral
Members there:
- Instructors and advisors: Alice and Sylvie.
- Students: Eugene, Fabio, Hoang Vu, Leila, Juliette, Pierre, Romain, Sean and Terry.