Team:Paris Saclay/Notebook/August/18

From 2014.igem.org

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Revision as of 14:56, 19 August 2014

Contents

Monday 18th August

Lab work

D - Lemon Scent

PCR of BBa_K517003

By Hoang Vu and Eugène

We realized a PCR to amplify the β-pinene synthase gene.

PCR of BBa_K517003
components volumes
DNA of GS 1 μl
green goTaq buffer 5X 10 μl
GoTaq enzyme 0.5 µl
H2O 29.5 µl
MgCl2 4 µl
dNTP 1µl
IPS68bis 2 µl
IPS69bis 2 µl

PCR of BBa_K762100

by Sean

Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on 8th August).

Primers used: iPS66 and iPS67.

PCR of pCola

"by Laetitia and Melanie"

Five tubes were prepared. Protocol was modified to match the one used for pCola (PCR conducted on 8th August).

Primers used : iPS91bis and iPS82

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