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Our Parts

The 2014 Exeter iGEM Team has submitted eight parts to the iGEM Registry this year. Four of them are basic parts while four of them are composite parts. Each of the four basic parts is a simple protein coding or regulatory sequence; they each have a complementary composite part in which they can act as

Basic Parts

BBa_K1398000 : XenB (Xenobiotic Reductase B)

Xenobiotic Reductase B, created for use as a Trinitrotoluene and Nitroglycerine degrading protein.

A monomeric flavin that can reduce certain nitro- groups to nitrate-. Increases the resistance of organisms to the toxic effects of nitrocompounds.

This sequence encodes for a protein (with an attached His (x6) Tag to allow for purification) and nothing else. It therefore requires regulatory sequences (promoter/RBS/terminator) to be added for expression. The protein has been codon-optimized for expression in E. coli.

BBa_K1398002 : NemA (N-ethylmaleimide reductase)

N-ethylmaleimide reductase, created for use as a Trinitrotoluene and Nitroglycerine degrading protein. NemA is a flavoprotein that primarily catalyses the reduction of N-ethylmaleimide (NEM), which is toxic to cell growth.

However, it is also involved in the degradation of other toxic compounds for their reuse in nitrogen metabolism. Some of these compounds include PETN, quinones and chromate. It increases the resistance of organisms to the toxic effects of these compounds.

This sequence only encodes for the protein, an attached His (x6) Tag to allow for purification and a double-STOP codon. It therefore requires regulatory sequences (promoter/RBS/terminator) to be added for expression. The protein has been codon-optimised for expression in E. coli.

BBa_K1398005 : NemR Upstream Intergenic Region

This sequence is found upstream of several NemR regulated genes. It was created for use as a TNT-detection mechanism.

It contains the bases from X+ to the start codon, which include a promoter and RBS. This region should allow regulation of gene products through the detection of TNT. If it is not present NemR will bind to a specified sequence of bases and inhibit transcription. If TNT is not present NemR will not bind and transcription will complete.

BBa_K1398008 : NemR Recognition Promoter

This sequences combines a high level constitutive promoter with the NemR binding box, which allows NemR to bind to DNA when no TNT is present in the cell. It was created for use as a TNT-detection mechanism. It combines BBa_J23100 with the NemR box to create a promoter that will theoretically have high levels of transcription when TNT is not present.

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