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Team:Exeter/LabBook
From 2014.igem.org
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<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%281%29_.JPG”>1</a></td> |
<td>30/06/14</td> <!-- date --> | <td>30/06/14</td> <!-- date --> | ||
<td>Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed.</td> <!-- desciption --> | <td>Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed.</td> <!-- desciption --> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%282%29_.JPG”>2</a></td> |
<td>31/06/14</td> | <td>31/06/14</td> | ||
<td>Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed./td> | <td>Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed./td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%283%29_.JPG”>3</a></td> |
<td>01/07/14</td> | <td>01/07/14</td> | ||
<td>List of promoters transformed, cont. Transformations were plated.</td> | <td>List of promoters transformed, cont. Transformations were plated.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%284%29_.JPG”>4</a></td> |
<td>02/07/14</td> | <td>02/07/14</td> | ||
<td>The previous day transformations were plated. </td> | <td>The previous day transformations were plated. </td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%285%29_.JPG”>5</a></td> |
<td>02/07/14</td> | <td>02/07/14</td> | ||
<td>Transformation of iGEM re-suspended promoters.</td> | <td>Transformation of iGEM re-suspended promoters.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%286%29_.JPG”>6</a></td> |
<td>02/07/14</td> | <td>02/07/14</td> | ||
<td>Preparation of competent E. coli cells</td> | <td>Preparation of competent E. coli cells</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%287%29_.JPG”>7</a></td> |
<td>03/07/14</td> | <td>03/07/14</td> | ||
<td>Promoter transformation results.</td> | <td>Promoter transformation results.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%288%29_.JPG”>8</a></td> |
<td>03/07/14</td> | <td>03/07/14</td> | ||
<td>E. coli growth rate.</td> | <td>E. coli growth rate.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%289%29_.JPG”>9</a></td> |
<td>04/07/14</td> | <td>04/07/14</td> | ||
<td>Transformation of promoters and gene constructs.</td> | <td>Transformation of promoters and gene constructs.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2810%29_.JPG”>10</a></td> |
<td>07/07/14</td> | <td>07/07/14</td> | ||
<td>Putting constructs into the iGEM vector and testing for transformation efficiency.</td> | <td>Putting constructs into the iGEM vector and testing for transformation efficiency.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2811%29_.JPG”>11</a></td> |
<td>08/07/14</td> | <td>08/07/14</td> | ||
<td>Repeat construct addition into iGEM vector and testing transformation efficiency.</td> | <td>Repeat construct addition into iGEM vector and testing transformation efficiency.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2812%29_.JPG”>12</a></td> |
<td>08/07/14</td> | <td>08/07/14</td> | ||
<td>Transforming constructs into Top10 and DH5.</td> | <td>Transforming constructs into Top10 and DH5.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2813%29_.JPG”>13</a></td> |
<td>09/07/14</td> | <td>09/07/14</td> | ||
<td>Results of Construction of iGEM vector.</td> | <td>Results of Construction of iGEM vector.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2814%29_.JPG”>14</a></td> |
<td>10/07/14</td> | <td>10/07/14</td> | ||
<td>A range of mini-preps</td> | <td>A range of mini-preps</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2815%29_.JPG”>15</a></td> |
<td>11/07/14</td> | <td>11/07/14</td> | ||
<td>Measuring the length of DNA.</td> | <td>Measuring the length of DNA.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2816%29_.JPG”>16</a></td> |
<td>11/07/14</td> | <td>11/07/14</td> | ||
<td>Measuring the length of DNA, cont.</td> | <td>Measuring the length of DNA, cont.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2817%29_.JPG”>17</a></td> |
<td>11/07/14</td> | <td>11/07/14</td> | ||
<td>Mini-prepping picked cultures containing promoters.</td> | <td>Mini-prepping picked cultures containing promoters.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2818%29_.JPG”>18</a></td> |
<td>11/07/14</td> | <td>11/07/14</td> | ||
<td>Parts sent for sequencing</td> | <td>Parts sent for sequencing</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2819%29_.JPG”>19</a></td> |
<td>11/07/14</td> | <td>11/07/14</td> | ||
<td>Linearizing DNA.</td> | <td>Linearizing DNA.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2820%29_.JPG”>20</a></td> |
<td>11/07/14</td> | <td>11/07/14</td> | ||
<td>Creating glycerol stocks.</td> | <td>Creating glycerol stocks.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2821%29_.JPG”>21</a></td> |
<td>14/07/14</td> | <td>14/07/14</td> | ||
<td>GFP and RFP resuspension and transformation.</td> | <td>GFP and RFP resuspension and transformation.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2822%29_.JPG”>22</a></td> |
<td>14/07/14</td> | <td>14/07/14</td> | ||
<td>GFP and RFP resuspension and transformation, cont.</td> | <td>GFP and RFP resuspension and transformation, cont.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2823%29_.JPG”>23</a></td> |
<td>16/07/14</td> | <td>16/07/14</td> | ||
<td>Mini-prepping GFP and RFP.</td> | <td>Mini-prepping GFP and RFP.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2824%29_.JPG”>24</a></td> |
<td>16/07/14</td> | <td>16/07/14</td> | ||
<td>GFP and RFP digestion/ligation to PSB1C3.</td> | <td>GFP and RFP digestion/ligation to PSB1C3.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2825%29_.JPG”>25</a></td> |
<td>16/07/14</td> | <td>16/07/14</td> | ||
<td>Plasmid digestions.</td> | <td>Plasmid digestions.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2826%29_.JPG”>26</a></td> |
<td>16/07/14</td> | <td>16/07/14</td> | ||
<td>RFP/GFP ligations.</td> | <td>RFP/GFP ligations.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2827%29_.JPG”>27</a></td> |
<td>16/07/14</td> | <td>16/07/14</td> | ||
<td>RFP/GFP ligations, cont.</td> | <td>RFP/GFP ligations, cont.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2828%29_.JPG”>28</a></td> |
<td>17/07/14</td> | <td>17/07/14</td> | ||
<td>Transformation of GFP/RFP with terminator.</td> | <td>Transformation of GFP/RFP with terminator.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2829%29_.JPG”>29</a></td> |
<td>18/07/14</td> | <td>18/07/14</td> | ||
<td>Making LB agar for promoters experiment.</td> | <td>Making LB agar for promoters experiment.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2830%29_.JPG”>30</a></td> |
<td>18/07/14</td> | <td>18/07/14</td> | ||
<td>Promoter experiment – quantities.</td> | <td>Promoter experiment – quantities.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2831%29_.JPG”>31</a></td> |
<td>18/07/14</td> | <td>18/07/14</td> | ||
<td>Promoter experiment – quantities, cont.</td> | <td>Promoter experiment – quantities, cont.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2832%29_.JPG”>32</a></td> |
<td>18/07/14</td> | <td>18/07/14</td> | ||
<td>Promoter experiment – quantities, cont. 2.</td> | <td>Promoter experiment – quantities, cont. 2.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2833%29_.JPG”>33</a></td> |
<td>21/07/14</td> | <td>21/07/14</td> | ||
<td>Promoter experiment preliminary timetable.</td> | <td>Promoter experiment preliminary timetable.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2834%29_.JPG”>34</a></td> |
<td>22/07/14</td> | <td>22/07/14</td> | ||
<td>Electrophoresis of RFP and GFP (with terminators).</td> | <td>Electrophoresis of RFP and GFP (with terminators).</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2835%29_.JPG”>35</a></td> |
<td>22/07/14</td> | <td>22/07/14</td> | ||
<td>Preparation of competent E. coli cells.</td> | <td>Preparation of competent E. coli cells.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2836%29_.JPG”>36</a></td> |
<td>23/07/14</td> | <td>23/07/14</td> | ||
<td>GFP/RFP + terminator liquid broths mini-prepped.</td> | <td>GFP/RFP + terminator liquid broths mini-prepped.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2837%29_.JPG”>37</a></td> |
<td>23/07/14</td> | <td>23/07/14</td> | ||
<td>Potassium Chromate preparation.</td> | <td>Potassium Chromate preparation.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2838%29_.JPG”>38</a></td> |
<td>23/07/14</td> | <td>23/07/14</td> | ||
<td>Transformation of other promoters.</td> | <td>Transformation of other promoters.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2839%29_.JPG”>39</a></td> |
<td>24/07/14</td> | <td>24/07/14</td> | ||
<td>Moving GFP+T and RFP+T onto AMP backbones.</td> | <td>Moving GFP+T and RFP+T onto AMP backbones.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2840%29_.JPG”>40</a></td> |
<td>24/07/14</td> | <td>24/07/14</td> | ||
<td>Ligation of GFP+T and RFP+T onto AMP backbones.</td> | <td>Ligation of GFP+T and RFP+T onto AMP backbones.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2841%29_.JPG”>41</a></td> |
<td>24/07/14</td> | <td>24/07/14</td> | ||
<td>Potassium Dichromate colorimetry and spectrophotometry.</td> | <td>Potassium Dichromate colorimetry and spectrophotometry.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2842%29_.JPG”>42</a></td> |
<td>28/07/14</td> | <td>28/07/14</td> | ||
<td>Plating Anderson RBSs.</td> | <td>Plating Anderson RBSs.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2843%29_.JPG”>43</a></td> |
<td>28/07/14</td> | <td>28/07/14</td> | ||
<td>Mini-prep of reporters, promoters and RBSs.</td> | <td>Mini-prep of reporters, promoters and RBSs.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2844%29_.JPG”>44</a></td> |
<td>29/07/14</td> | <td>29/07/14</td> | ||
<td>COSHH Form created for Nitroglycerine experiments.</td> | <td>COSHH Form created for Nitroglycerine experiments.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2845%29_.JPG”>45</a></td> |
<td>30/07/14</td> | <td>30/07/14</td> | ||
<td>Cr(VI) / Ligand experimentation.</td> | <td>Cr(VI) / Ligand experimentation.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2846%29_.JPG”>46</a></td> |
<td>31/07/14</td> | <td>31/07/14</td> | ||
<td>Mini-prep of constructs.</td> | <td>Mini-prep of constructs.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2847%29_.JPG”>47</a></td> |
<td>31/07/14</td> | <td>31/07/14</td> | ||
<td>Digestion and ligation of promoter/RBS combos.</td> | <td>Digestion and ligation of promoter/RBS combos.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2848%29_.JPG”>48</a></td> |
<td>31/07/14</td> | <td>31/07/14</td> | ||
<td>Oligo preparation.</td> | <td>Oligo preparation.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2849%29_.JPG”>49</a></td> |
<td>31/07/14</td> | <td>31/07/14</td> | ||
<td>Ligation of promoter and RBs digestions.</td> | <td>Ligation of promoter and RBs digestions.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2850%29_.JPG”>50</a></td> |
<td>01/08/14</td> | <td>01/08/14</td> | ||
<td>Digestion and Ligation of constructs 004 and 007.</td> | <td>Digestion and Ligation of constructs 004 and 007.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2851%29_.JPG”>51</a></td> |
<td>01/08/14</td> | <td>01/08/14</td> | ||
<td>Digestion and Ligation of constructs 004 and 007, cont.</td> | <td>Digestion and Ligation of constructs 004 and 007, cont.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2852%29_.JPG”>52</a></td> |
<td>05/08/14</td> | <td>05/08/14</td> | ||
<td>Colonies picked and mini-prepped.</td> | <td>Colonies picked and mini-prepped.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2853%29_.JPG”>53</a></td> |
<td>05/08/14</td> | <td>05/08/14</td> | ||
<td>Colonies picked and mini-prepped, cont.</td> | <td>Colonies picked and mini-prepped, cont.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2854%29_.JPG”>54</a></td> |
<td>06/08/14</td> | <td>06/08/14</td> | ||
<td>Testing promoter/RBS combination efficiency.</td> | <td>Testing promoter/RBS combination efficiency.</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2855%29_.JPG”>55</a></td> |
<td>06/08/14</td> | <td>06/08/14</td> | ||
<td>Testing promoter/RBS combination efficiency, cont.</td> | <td>Testing promoter/RBS combination efficiency, cont.</td> | ||
| Line 351: | Line 351: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2856%29_.JPG”>56</a></td> |
<td>07/08/14</td> | <td>07/08/14</td> | ||
<td>Picking colonies from promoter/RBS combination transformations. </td> | <td>Picking colonies from promoter/RBS combination transformations. </td> | ||
| Line 357: | Line 357: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2857%29_.JPG”>57</a></td> |
<td>07/08/14</td> | <td>07/08/14</td> | ||
<td>T. Cam experiment.</td> | <td>T. Cam experiment.</td> | ||
| Line 363: | Line 363: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2858%29_.JPG”>58</a></td> |
<td>11/08/14</td> | <td>11/08/14</td> | ||
<td>Promoter/RBS combinations again.</td> | <td>Promoter/RBS combinations again.</td> | ||
| Line 369: | Line 369: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2859%29_.JPG”>59</a></td> |
<td>11/08/14</td> | <td>11/08/14</td> | ||
<td>Promoter/RBS combinations again, cont.</td> | <td>Promoter/RBS combinations again, cont.</td> | ||
| Line 375: | Line 375: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2860%29_.JPG”>60</a></td> |
<td>11/08/14</td> | <td>11/08/14</td> | ||
<td>Inoculation of enzymes for construct 001 and 003.</td> | <td>Inoculation of enzymes for construct 001 and 003.</td> | ||
| Line 381: | Line 381: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2861%29_.JPG”>61</a></td> |
<td>12/08/14</td> | <td>12/08/14</td> | ||
<td>Inoculation of enzymes for construct 001 and 003, cont.</td> | <td>Inoculation of enzymes for construct 001 and 003, cont.</td> | ||
| Line 387: | Line 387: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2862%29_.JPG”>62</a></td> |
<td>12/08/14</td> | <td>12/08/14</td> | ||
<td>Transformation of a sample promoter/RBS combination.</td> | <td>Transformation of a sample promoter/RBS combination.</td> | ||
| Line 393: | Line 393: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2863%29_.JPG”>63</a></td> |
<td>12/08/14</td> | <td>12/08/14</td> | ||
<td>T. Cam experiment for solvents and ether.</td> | <td>T. Cam experiment for solvents and ether.</td> | ||
| Line 399: | Line 399: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2864%29_.JPG”>64</a></td> |
<td>13/08/14</td> | <td>13/08/14</td> | ||
<td>Combination transformation results and construct testing.</td> | <td>Combination transformation results and construct testing.</td> | ||
| Line 405: | Line 405: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td><a href=“Exeter_iGEM_2014_Lab_Book_Write-Up_%2865%29_.JPG”>65</a></td> |
<td>13/08/14</td> | <td>13/08/14</td> | ||
<td>Putting oligos into GFP and RFP.</td> | <td>Putting oligos into GFP and RFP.</td> | ||
Revision as of 01:08, 18 October 2014
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Lab Book
On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.
Page No. Date: Description: Carried out by: 1 30/06/14 Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed. Martyn Bennet, Beth Hickton 2 31/06/14 Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed./td> Beth Hickton, Edward Muir 3 01/07/14 List of promoters transformed, cont. Transformations were plated. Beth Hickton, Martyn Bennet 4 02/07/14 The previous day transformations were plated. Beth Hickton 5 02/07/14 Transformation of iGEM re-suspended promoters. Beth Hickton 6 02/07/14 Preparation of competent E. coli cells Beth Hickton 7 03/07/14 Promoter transformation results. Jessica Rollit, Martyn Bennet 8 03/07/14 E. coli growth rate. Edward Muir, Beth Hickton 9 04/07/14 Transformation of promoters and gene constructs. Edward Muir, Beth Hickton 10 07/07/14 Putting constructs into the iGEM vector and testing for transformation efficiency. Martyn Bennet, Edward Muir, Beth Hickton 11 08/07/14 Repeat construct addition into iGEM vector and testing transformation efficiency. Edward Muir, Beth Hickton, Martyn Bennet 12 08/07/14 Transforming constructs into Top10 and DH5. Beth Hickton, Martyn Bennet, Edward Muir 13 09/07/14 Results of Construction of iGEM vector. Jessica Rollit, Beth Hickton 14 10/07/14 A range of mini-preps Jessica Rollit, Beth Hickton 15 11/07/14 Measuring the length of DNA. Edward Muir 16 11/07/14 Measuring the length of DNA, cont. Edward Muir 17 11/07/14 Mini-prepping picked cultures containing promoters. Callum Bailey, Elize Hernandez 18 11/07/14 Parts sent for sequencing Callum Bailey, Elize Hernandez 19 11/07/14 Linearizing DNA. Edward Muir 20 11/07/14 Creating glycerol stocks. Beth Hickton, Max Smart 21 14/07/14 GFP and RFP resuspension and transformation. Edward Muir, Martyn Bennet, Beth Hickton 22 14/07/14 GFP and RFP resuspension and transformation, cont. Beth Hickton, Edward Muir 23 16/07/14 Mini-prepping GFP and RFP. Jessica Rollit, Edward Muir, Fran Penrose 24 16/07/14 GFP and RFP digestion/ligation to PSB1C3. Beth Hickton, Edward Muir 25 16/07/14 Plasmid digestions. Edward Muir, Callum Bailey, Benjamin Miller 26 16/07/14 RFP/GFP ligations. Edward Muir 27 16/07/14 RFP/GFP ligations, cont. Edward Muir 28 17/07/14 Transformation of GFP/RFP with terminator. Edward Muir 29 18/07/14 Making LB agar for promoters experiment. Katie Pearce 30 18/07/14 Promoter experiment – quantities. Edward Muir 31 18/07/14 Promoter experiment – quantities, cont. Edward Muir 32 18/07/14 Promoter experiment – quantities, cont. 2. Edward Muir 33 21/07/14 Promoter experiment preliminary timetable. Edward Muir 34 22/07/14 Electrophoresis of RFP and GFP (with terminators). Edward Muir 35 22/07/14 Preparation of competent E. coli cells. Beth Hickton, Peter Reader 36 23/07/14 GFP/RFP + terminator liquid broths mini-prepped. Beth Hickton, Peter Reader, Jessica Rollit 37 23/07/14 Potassium Chromate preparation. Peter Reader, Benjamin Miller, Beth Hickton 38 23/07/14 Transformation of other promoters. Edward Muir 39 24/07/14 Moving GFP+T and RFP+T onto AMP backbones. Edward Muir, Katie Pearce 40 24/07/14 Ligation of GFP+T and RFP+T onto AMP backbones. Edward Muir, Beth Hickton 41 24/07/14 Potassium Dichromate colorimetry and spectrophotometry. Beth Hickton, Edward Muir 42 28/07/14 Plating Anderson RBSs. Beth Hickton, Edward Muir, Elize Hernandez 43 28/07/14 Mini-prep of reporters, promoters and RBSs. Beth Hickton, Elize Hernandez, Edward Muir 44 29/07/14 COSHH Form created for Nitroglycerine experiments. Beth Hickton, Peter Reader, Benjamin Miller 45 30/07/14 Cr(VI) / Ligand experimentation. Peter Reader 46 31/07/14 Mini-prep of constructs. Edward Muir, Elize Hernandez, Beth Hickton 47 31/07/14 Digestion and ligation of promoter/RBS combos. Edward Muir, Callum Bailey 48 31/07/14 Oligo preparation. Edward Muir, Callum Bailey 49 31/07/14 Ligation of promoter and RBs digestions. Edward Muir, Callum Bailey, Benjamin Miller 50 01/08/14 Digestion and Ligation of constructs 004 and 007. Edward Muir, Jessica Rollit, Beth Hickton 51 01/08/14 Digestion and Ligation of constructs 004 and 007, cont. Edward Muir 52 05/08/14 Colonies picked and mini-prepped. Callum Bailey 53 05/08/14 Colonies picked and mini-prepped, cont. Callum Bailey 54 06/08/14 Testing promoter/RBS combination efficiency. Callum Bailey, Edward Muir 55 06/08/14 Testing promoter/RBS combination efficiency, cont. Callum Bailey, Edward Muir 56 07/08/14 Picking colonies from promoter/RBS combination transformations. Edward Muir, Callum Bailey 57 07/08/14 T. Cam experiment. Edward Muir, Callum Bailey 58 11/08/14 Promoter/RBS combinations again. Edward Muir, Callum Bailey 59 11/08/14 Promoter/RBS combinations again, cont. Edward Muir, Callum Bailey 60 11/08/14 Inoculation of enzymes for construct 001 and 003. Edward Muir, Callum Bailey 61 12/08/14 Inoculation of enzymes for construct 001 and 003, cont. Edward Muir, Callum Bailey 62 12/08/14 Transformation of a sample promoter/RBS combination. Callum Bailey 63 12/08/14 T. Cam experiment for solvents and ether. Edward Muir 64 13/08/14 Combination transformation results and construct testing. Edward Muir 65 13/08/14 Putting oligos into GFP and RFP. Edward Muir These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.
Exeter | ERASE
ERASE-The Game!
