Team:Exeter/LabBook

From 2014.igem.org

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<h1>Lab Book</h1>
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<p>These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.</p>
<p>These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.</p>
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Revision as of 01:03, 18 October 2014

Exeter | ERASE

Lab Book

On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.


Page No. Date: Description: Carried out by:
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%281%29_.JPG | 1]] 30/06/14 Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed. Martyn Bennet, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%282%29_.JPG | 2]] 31/06/14 Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed./td> Beth Hickton, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%283%29_.JPG | 3]] 01/07/14 List of promoters transformed, cont. Transformations were plated. Beth Hickton, Martyn Bennet
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%284%29_.JPG | 4]] 02/07/14 The previous day transformations were plated. Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%285%29_.JPG | 5]] 02/07/14 Transformation of iGEM re-suspended promoters. Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%286%29_.JPG | 6]] 02/07/14 Preparation of competent E. coli cells Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%287%29_.JPG | 7]] 03/07/14 Promoter transformation results. Jessica Rollit, Martyn Bennet
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%288%29_.JPG | 8]] 03/07/14 E. coli growth rate. Edward Muir, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%289%29_.JPG | 9]] 04/07/14 Transformation of promoters and gene constructs. Edward Muir, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2810%29_.JPG | 10]] 07/07/14 Putting constructs into the iGEM vector and testing for transformation efficiency. Martyn Bennet, Edward Muir, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2811%29_.JPG | 11]] 08/07/14 Repeat construct addition into iGEM vector and testing transformation efficiency. Edward Muir, Beth Hickton, Martyn Bennet
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2812%29_.JPG | 12]] 08/07/14 Transforming constructs into Top10 and DH5. Beth Hickton, Martyn Bennet, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2813%29_.JPG | 13]] 09/07/14 Results of Construction of iGEM vector. Jessica Rollit, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2814%29_.JPG | 14]] 10/07/14 A range of mini-preps Jessica Rollit, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2815%29_.JPG | 15]] 11/07/14 Measuring the length of DNA. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2816%29_.JPG | 16]] 11/07/14 Measuring the length of DNA, cont. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2817%29_.JPG | 17]] 11/07/14 Mini-prepping picked cultures containing promoters. Callum Bailey, Elize Hernandez
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2818%29_.JPG | 18]] 11/07/14 Parts sent for sequencing Callum Bailey, Elize Hernandez
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2819%29_.JPG | 19]] 11/07/14 Linearizing DNA. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2820%29_.JPG | 20]] 11/07/14 Creating glycerol stocks. Beth Hickton, Max Smart
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2821%29_.JPG | 21]] 14/07/14 GFP and RFP resuspension and transformation. Edward Muir, Martyn Bennet, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2822%29_.JPG | 22]] 14/07/14 GFP and RFP resuspension and transformation, cont. Beth Hickton, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2823%29_.JPG | 23]] 16/07/14 Mini-prepping GFP and RFP. Jessica Rollit, Edward Muir, Fran Penrose
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2824%29_.JPG | 24]] 16/07/14 GFP and RFP digestion/ligation to PSB1C3. Beth Hickton, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2825%29_.JPG | 25]] 16/07/14 Plasmid digestions. Edward Muir, Callum Bailey, Benjamin Miller
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2826%29_.JPG | 26]] 16/07/14 RFP/GFP ligations. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2827%29_.JPG | 27]] 16/07/14 RFP/GFP ligations, cont. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2828%29_.JPG | 28]] 17/07/14 Transformation of GFP/RFP with terminator. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2829%29_.JPG | 29]] 18/07/14 Making LB agar for promoters experiment. Katie Pearce
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2830%29_.JPG | 30]] 18/07/14 Promoter experiment – quantities. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2831%29_.JPG | 31]] 18/07/14 Promoter experiment – quantities, cont. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2832%29_.JPG | 32]] 18/07/14 Promoter experiment – quantities, cont. 2. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2833%29_.JPG | 33]] 21/07/14 Promoter experiment preliminary timetable. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2834%29_.JPG | 34]] 22/07/14 Electrophoresis of RFP and GFP (with terminators). Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2835%29_.JPG | 35]] 22/07/14 Preparation of competent E. coli cells. Beth Hickton, Peter Reader
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2836%29_.JPG | 36]] 23/07/14 GFP/RFP + terminator liquid broths mini-prepped. Beth Hickton, Peter Reader, Jessica Rollit
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2837%29_.JPG | 37]] 23/07/14 Potassium Chromate preparation. Peter Reader, Benjamin Miller, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2838%29_.JPG | 38]] 23/07/14 Transformation of other promoters. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2839%29_.JPG | 39]] 24/07/14 Moving GFP+T and RFP+T onto AMP backbones. Edward Muir, Katie Pearce
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2840%29_.JPG | 40]] 24/07/14 Ligation of GFP+T and RFP+T onto AMP backbones. Edward Muir, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2841%29_.JPG | 41]] 24/07/14 Potassium Dichromate colorimetry and spectrophotometry. Beth Hickton, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2842%29_.JPG | 42]] 28/07/14 Plating Anderson RBSs. Beth Hickton, Edward Muir, Elize Hernandez
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2843%29_.JPG | 43]] 28/07/14 Mini-prep of reporters, promoters and RBSs. Beth Hickton, Elize Hernandez, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2844%29_.JPG | 44]] 29/07/14 COSHH Form created for Nitroglycerine experiments. Beth Hickton, Peter Reader, Benjamin Miller
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2845%29_.JPG | 45]] 30/07/14 Cr(VI) / Ligand experimentation. Peter Reader
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2846%29_.JPG | 46]] 31/07/14 Mini-prep of constructs. Edward Muir, Elize Hernandez, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2847%29_.JPG | 47]] 31/07/14 Digestion and ligation of promoter/RBS combos. Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2848%29_.JPG | 48]] 31/07/14 Oligo preparation. Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2849%29_.JPG | 49]] 31/07/14 Ligation of promoter and RBs digestions. Edward Muir, Callum Bailey, Benjamin Miller
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2850%29_.JPG | 50]] 01/08/14 Digestion and Ligation of constructs 004 and 007. Edward Muir, Jessica Rollit, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2851%29_.JPG | 51]] 01/08/14 Digestion and Ligation of constructs 004 and 007, cont. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2852%29_.JPG | 52]] 05/08/14 Colonies picked and mini-prepped. Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2853%29_.JPG | 53]] 05/08/14 Colonies picked and mini-prepped, cont. Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2854%29_.JPG | 54]] 06/08/14 Testing promoter/RBS combination efficiency. Callum Bailey, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2855%29_.JPG | 55]] 06/08/14 Testing promoter/RBS combination efficiency, cont. Callum Bailey, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2856%29_.JPG | 56]] 07/08/14 Picking colonies from promoter/RBS combination transformations. Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2857%29_.JPG | 57]] 07/08/14 T. Cam experiment. Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2858%29_.JPG | 58]] 11/08/14 Promoter/RBS combinations again. Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2859%29_.JPG | 59]] 11/08/14 Promoter/RBS combinations again, cont. Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2860%29_.JPG | 60]] 11/08/14 Inoculation of enzymes for construct 001 and 003. Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2861%29_.JPG | 61]] 12/08/14 Inoculation of enzymes for construct 001 and 003, cont. Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2862%29_.JPG | 62]] 12/08/14 Transformation of a sample promoter/RBS combination. Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2863%29_.JPG | 63]] 12/08/14 T. Cam experiment for solvents and ether. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2864%29_.JPG | 64]] 13/08/14 Combination transformation results and construct testing. Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2865%29_.JPG | 65]] 13/08/14 Putting oligos into GFP and RFP. Edward Muir

These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.

Exeter | ERASE

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