User contributions

From 2014.igem.org

(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)

• 20:48, 17 October 2014 (diff | hist) Team:Harvard BioDesign/Notebook ‎
• 20:48, 17 October 2014 (diff | hist) Team:Harvard BioDesign/Notebook ‎
• 20:43, 17 October 2014 (diff | hist) N Harvard BioDesign/16 October 2014 ‎ (Created page with "Sheared curli off of CsgA Constructs Ran Congo Red spin down on constructs (-ctrl, +ctrl, engineered constructs)-->plate reader") (top)
• 20:42, 17 October 2014 (diff | hist) N Harvard BioDesign/15 October 2014 ‎ (Created page with "Plated CsgA constructs and also spotted WT-CsgA and Spytag-CsgA Grew up flask of pHBD46 for lysing") (top)
• 20:42, 17 October 2014 (diff | hist) N Harvard BioDesign/14 October 2014 ‎ (Created page with "Grew more CsgA constructs in YESCA and made more YESCA IPTG Carb plates") (top)
• 20:42, 17 October 2014 (diff | hist) N Harvard BioDesign/7 October 2014 ‎ (Created page with "Transfer starting culture of pHBD46 to 100mL (LB+Carb)") (top)
• 20:41, 17 October 2014 (diff | hist) N Harvard BioDesign/6 October 2014 ‎ (Created page with "Miniprep biobrick part Grew more CsgA constructs for congo red spin down and incubation with pHBD46 Submitted sequencing of biobrick part") (top)
• 20:41, 17 October 2014 (diff | hist) N Harvard BioDesign/5 October 2014 ‎ (Created page with "Picked colonies from biobrick transformation") (top)
• 20:41, 17 October 2014 (diff | hist) N Harvard BioDesign/4 October 2014 ‎ (Created page with "Transformed and plated biobrick part") (top)
• 20:39, 17 October 2014 (diff | hist) Team:Harvard BioDesign/Notebook ‎
• 20:39, 17 October 2014 (diff | hist) N Harvard BioDesign/16 September 2014 ‎ (Created page with "PCR’ed parts for gibson of chromoprotein constructs with spycatcher and linker (see PCR planning page). Gibsoned and sequenced these parts. Submitted for sequencing.") (top)
• 20:38, 17 October 2014 (diff | hist) N Harvard BioDesign/14 September 2014 ‎ (Created page with "Ordered biobricks for 24 amino acid linker and primers to put part from pHBD46 into the iGEM backbone.") (top)
• 20:37, 17 October 2014 (diff | hist) N Harvard BioDesign/30 September 2014 ‎ (Created page with "Received Sequencing results") (top)
• 20:37, 17 October 2014 (diff | hist) N Harvard BioDesign/29 September 2014 ‎ (Created page with "-Clean up plates -Grow up 100mL cultures of pHBD46, pHBD48 -Run PCR products on gel/ gel extract/ PCR purify (potentially gibson & transform if you have time)") (top)
• 20:37, 17 October 2014 (diff | hist) N Harvard BioDesign/28 September 2014 ‎ (Created page with "Plated more CsgA--This is growing up on the bench and will be ready for congo red/ incubation on Tuesday (used pHBD48 as neg ctrl because our large yesca plates are only carb--i ...") (top)
• 20:37, 17 October 2014 (diff | hist) N Harvard BioDesign/13 September 2014 ‎ (Created page with "Inoculated 50ml cultures of 46 & 48 Inoculated 5ml cultures of PHL -, 53 (Csga), 54 (Csga-spy)") (top)
• 20:36, 17 October 2014 (diff | hist) N Harvard BioDesign/11 September 2014 ‎ (Created page with "Purified pHBD48 and pHBD46 Inoculated more pHBD46 and pHBD48") (top)
• 20:36, 17 October 2014 (diff | hist) N Harvard BioDesign/10 September 2014 ‎ (Created page with "Made YESCA-IPTG-CRB plates") (top)
• 20:35, 17 October 2014 (diff | hist) Team:Harvard BioDesign/Notebook ‎
• 20:35, 17 October 2014 (diff | hist) N Harvard BioDesign/7 August 2014 ‎ (Created page with "Ran SDS Page gel on incubation products of CsgA Spy Tag and Chromoprotein-Spy Catcher Gibsoned parts of pHBD 68, pHBD 69, pHBD 70, pHBD 72, pHBD 73 and pHBD 74 Transformed Gibson...") (top)
• 20:34, 17 October 2014 (diff | hist) N Harvard BioDesign/4 August 2014 ‎ (Created page with "Congo red spin down on induced LSR10 cells with SpyTag, SynZIP #1 and SynZIP #5 Incubate cells expressing SpyTag on their curli monomers with Chromoprotein-SpyTag lysate Resul...") (top)
• 20:33, 17 October 2014 (diff | hist) N Harvard BioDesign/1 August 2014 ‎ (Created page with "Picked colonies containing pHBD 54, 55, 56, and 57 (CsgA tagged with SpyTag, Synzip 1, Synzip 4, and SynZip 5 respectively) and inoculated them in LSR10s and PHLs. After cells r...") (top)
• 20:32, 17 October 2014 (diff | hist) N Harvard BioDesign/31 July 2014 ‎ (Created page with "Sequencing results came back viable for…. SynZIP1_C SynZIP4_A,B,C SynZIP5 (there is a deletion in flag tag, but the insert is there) SpyTag A Received HBD 108-111 (Primers to ...") (top)
• 20:32, 17 October 2014 (diff | hist) N Harvard BioDesign/30 July 2014 ‎ (Created page with "Ran gel to verify PCR products that we Gibsoned yesterday Backbone (for SynZIPs)- CsgA_48Linker_Flag Backbone (for SpyTag)- CsgA_48Linker Grow up colonies picked from plates wit...") (top)
• 20:32, 17 October 2014 (diff | hist) N Harvard BioDesign/29 July 2014 ‎ (Created page with "Colonies from Gibson of pBbE1a backbone- CsgA- 48 Linkiner- SynZIP1 did not grow Error amplifying the backbone HBD 5 and 7 were used as primers (point inward towards CsgA) Primer...") (top)
• 20:31, 17 October 2014 (diff | hist) N Harvard BioDesign/28 July 2014 ‎ (Created page with "Received Synzips 1 and 5 Gibsoned Synzip #1 and Synzip #2 into the CsgA-48 linker backbone Transformed Synzip #1 into Mach I cells * Synzip #5 ← → Synzip #6 * Synzip #1 ...") (top)
• 20:31, 17 October 2014 (diff | hist) N Harvard BioDesign/25 July 2014 ‎ (Created page with "Sequencing results The stop codon was missing from the ordered oligonucleotide blocks that were used for sequencing Miniprepped 11 Mach I- SpyTag- CsgA colonies growing up from ...") (top)
• 20:31, 17 October 2014 (diff | hist) N Harvard BioDesign/24 July 2014 ‎ (Created page with "Picked colonies from July 23rd transformations to grow up Place in 3 milliliters of LB + carb Picked 11 colonies to grow Add 7 milliliters of LB+ carb to the already growing tr...") (top)
• 20:30, 17 October 2014 (diff | hist) Harvard BioDesign/23 July 2014 ‎ (top)
• 20:29, 17 October 2014 (diff | hist) N Harvard BioDesign/23 July 2014 ‎ (Created page with "Sequencing results were not good The plasmid did not have the insert")
• 20:28, 17 October 2014 (diff | hist) N Harvard BioDesign/22 July 2014 ‎ (Created page with "Waited the whole day for the Mach I cells to grow up, but it seemed unsuccessful") (top)
• 20:28, 17 October 2014 (diff | hist) N Harvard BioDesign/21 July 2014 ‎ (Created page with "Grew up colonies from transformation plates: Mach I cells with SpyTag- CsgA Only one of the plates really had colonies. Not quite sure why the growth was so small? Plated 100 mi...") (top)
• 20:26, 17 October 2014 (diff | hist) Harvard BioDesign/18 July 2014 ‎ (top)
• 20:26, 17 October 2014 (diff | hist) N Harvard BioDesign/18 July 2014 ‎ (Created page with "Picked colonies from pHBD45, pHBD1 and pHBD2 transformations from previous day. Mini-prepped pHBD45 Ran PCR fragments for the gibson of pHBD40 from previous day on a gel, gel e...")
• 20:22, 17 October 2014 (diff | hist) N Harvard BioDesign/17 July 2014 ‎ (Created page with "→ No LB/25mL tubes...need to pick colonies tomorrow PCR’ed gibson fragments for pHBD40 with new primers (HBD71-74)") (top)
• 20:22, 17 October 2014 (diff | hist) N Harvard BioDesign/16 July 2014 ‎ (Created page with "Received pDEST14_His6_Spycatcher plasmid (pHBD45) from Peter→ transformed into MACH1s and plated to make our own stocks Transformed pHBD1 and pHBD2 into LSR10 & plated for cry...") (top)
• 20:22, 17 October 2014 (diff | hist) N Harvard BioDesign/10 July 2014 ‎ (Created page with "Transformed successful Gibsons: Chi C and CBD1_1 into LSR10 cells Grew up Turbo and LSR10 cells for a biocompatibility assay PCRed F12 and F48 backbones to grow stocks for future...") (top)
• 20:21, 17 October 2014 (diff | hist) N Harvard BioDesign/9 July 2014 ‎ (Created page with "PCRed plasmid backbones with the F12 and F48 linkers In order to perform more Gibson reactions Ran a gel on the PCRed backbone Assessed results of biocompatibility assay 1 ---...") (top)
• 20:21, 17 October 2014 (diff | hist) Harvard BioDesign/8 July 2014 ‎ (top)
• 20:20, 17 October 2014 (diff | hist) N Harvard BioDesign/8 July 2014 ‎ (Created page with "Gel extracted parts for HybB + SB3K3 for Gibson. Ran Gibson. Transformed. Picked colonies from PCR of pHBD39-41 to clone out prefix. Only pHBD41 had many colonies. Picked 2 col...")
• 20:19, 17 October 2014 (diff | hist) N Harvard BioDesign/7 July 2014 ‎ (Created page with "Ran PCR to remove prefix from pHBD39-41. Gel extraction only gave good concentration for pHBD41. Transformed all into MACH1 cells + plated @ 10uL and 60uL. PCR’ed HybB promot...") (top)
• 20:18, 17 October 2014 (diff | hist) N Harvard BioDesign/2 July 2014 ‎ (Created page with "We began minipreps of pHBD 1, 2, 43, and 44. We then ran our PCRs of fragments of pHBD 26 and 28 from yesterday on a gel: pHBD 18 and 35 were successfully PCRed, so those were ...") (top)
• 20:18, 17 October 2014 (diff | hist) N Harvard BioDesign/1 July 2014 ‎ (Created page with "Our LacO hypothesis was rejected as none of the IPTG induced cultures showed any coloring. However, we noticed that one of our plates with pHBD44 had blue colonies! Even though ...") (top)
• 20:17, 17 October 2014 (diff | hist) Harvard BioDesign/30 June 2014 ‎ (top)
• 20:16, 17 October 2014 (diff | hist) N Harvard BioDesign/30 June 2014 ‎ (Created page with "Promoter Cloning (HSP & ASP into SB3K3 backbone): We looked at our cultures that we placed in the 25 C and 30 C shakers over the weekend, but there was no color. We weren’t su...")
• 20:14, 17 October 2014 (diff | hist) N Harvard BioDesign/27 June 2014 ‎ (Created page with "We began minipreps on the following: Our retransformations of pHBD 5 and 35 Our gibsons of pHBD 39, 40, 41, 42, and 44. We finished minipreps of all of them. We also innoculated...") (top)
• 20:13, 17 October 2014 (diff | hist) N Harvard BioDesign/26 June 2014 ‎ (Created page with "We miniprepped pHBD 21 and 24. We retransformed pHBD 5 and 35. pHBD 37 arc’ed so we decided to pick from the original biobrick plate tomorrow morning when we inoculate culture...") (top)
• 20:13, 17 October 2014 (diff | hist) N Harvard BioDesign/25 June 2014 ‎ (Created page with "Finished the minipreps of BBa_K274200, BBa_I765001, pHBD4, BBa_K1073023, BBa_K1033931, and BBa_K1033929. Plated Gibson transformations of pHBD24 and pHBD21 had few (1-2) colonie...") (top)
• 20:12, 17 October 2014 (diff | hist) N Harvard BioDesign/24 June 2014 ‎ (Created page with "We picked up more stabs from iGEM HQ. We got three new chromoproteins: BBa_K1073023 (eforRed [pink]), BBa_K1033931 (amilGFP [yellow]), and BBa_K1033929 (aeBlue [indigo]). While w...") (top)
• 20:11, 17 October 2014 (diff | hist) N Harvard BioDesign/23 June 2014 ‎ (Created page with "Made electrocompotent MACH1 and PHL cells We ran the pbBE1a backbone from yesterday on a gel and this time we got bands We gel purified the backbone and hybB insert PCR produc...") (top)

(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)